The fluid flow velocity stands as a pivotal parameter with a great influence on the mutual interaction between the fluid domain and structure. This paper focuses on structural deformation, structural velocity, von-Mises stress and frequency response of a fixed-free end beam using two-way coupled fluid-structure interaction within ANSYS Workbench. The parameters such as deformed fluid pressure and velocity were analysed across three diverse flow regimes: laminar, transitional and turbulent – each aligned with distinct fluid velocities. The outcomes show that the total deformation, velocity and von-Mises stress distribution of the fixed-free end beam increase as the inlet fluid velocity increases. As a result, the pressure and velocity distribution in the fluid flow which receiving the resultant or leftover structure deflection also increases as the incoming fluid velocity increases. Furthermore, the analysis probes the frequency response in turbulent flow conditions reveals higher values compared to laminar and transitional flows, albeit within the same natural frequency domain. This observation marks significant vibrational characteristics found in the presence of turbulent flow dynamics.