Responses of red shiners, Notropis lutrensis, to water conditioned by the presence of conspecifics were evaluated in laboratory preference chambers. Fish from the South Canadian River (Arkansas River drainage) and Lake Texoma (Red River drainage), tested under different conditions at different times of year, all exhibited a strong attraction to water previously occupied by conspecifics. Olfaction as a cue to habitat selection is discussed with respect to the known ecology of the species. Some fishes of the turbid streams of the Great Plains have highly developed olfactory senses (Moore 1950). Notropis lutrensis (red shiner), one of the most abundant Great Plains species, can use physico-chemical and auditory stimuli in selecting habitat or locating mates (Delco 1960, Matthews and Hill 1979), but its response to olfactory stimuli has not been reported previously. Our field studies in the South Canadian River near Norman, Oklahoma, suggested that odor might also provide this species a cue to habitat selection, resulting in their attraction to water preconditioned by the presence of conspecifics. In October 1976, we collected 19,182 N. Iutrensis in a ca. 600 m section of the South Canadian River. Of those, 90.7% were taken in two backwater pools (each ca. 20 x 5 m) located near the main river channel (Matthews and Hill 1979). In addition to these collections, repeated visits to the site indicated use of the backwaters by large numbers of red shiners throughout October. We hypothesized that the shiners concentrated in the backwaters, despite adequate water for greater dispersal, because those areas presented favorable physico-chemical conditions. The backwaters were relatively stable thermally, fluctuating 1-2?C daily, compared to fluctuation of up to 12?C daily at mainstream locations. Additionally, diurnal pH in the backwaters (8.2-8.5) was closest to the pH selected by N. lutrensis in the laboratory (Matthews and Hill 1979), and pH in the mainstream (8.7-9.5) approached values stressful to this species (Matthews and Hill 1977). During the day, sharp temperature and pH gradients, which shiners could have used as habitat cues, existed between the mainstream and backwaters. At night, by contrast, no marked gradients existed, yet the thousands of N. lutrensis remained in the backwaters, not dispersing into nearby standing-water pools of the river mainstream. This behavior suggested that some factor not detected by our measurements, e.g., conditioning of backwaters by the presence of conspecifics, played a role in habitat selection by the species. The crowding of thousands of individuals into small pools undoubtedly resulted in accumulation of odors of mucous, excretory products, or other exudates. The attraction of fishes to water having the odor of conspecifics is well documented (Kleerekoper 1969, Fine et al. 1977).
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