Evaluation of the temperature profile induced by tunnel fire is of great significance for fire detection and structural damage assessment. In this work, a special plume model, namely, the “plume bifurcation” is introduced, and its impact on the temperature distribution is analysed theoretically. Full-scale tunnel fire experiments and numerical simulations are conducted to investigate the plume evolution under various heat release rates and ventilation conditions. Results show that the plume will sperate into two sub-streams under strong ventilation condition, resulting in the multi-dimensional plume movement pattern when rising. The sub-plume impingement point location, quantified by the longitudinal vertical deflection angle θv and horizontal bifurcation angle θh, is correlated with the fire heat release rate and ventilation condition. The plume bifurcation angle is 0 when the dimensionless ventilation velocity V′≤0.33, marking a single-stream plume; and rapidly increase and then slowly decrease with V′ when V′>0.33, representing the sudden occurrence and decay trend of plume bifurcation with the ventilation velocity. Finally, a novel bifurcated plume-based model to estimate the maximum ceiling temperature is proposed. This study reveals the role of multi-dimensional smoke movement in reshaping the temperature field, providing new perspectives for a deeper understanding of the smoke transport behaviour in longitudinally ventilated tunnel fires.