Fingertip Capillary Blood Research Articles

Comparison of lactate measurements from earlobe and fingertip capillary blood using Biosen S-Line and lactate scout analyzers.

This study aimed to compare variations between the earlobe and fingertip sampling sites in exercises dominated by upper body muscle exertion. It also sought to investigate capillary blood lactate differences between Lactate Scout 4 (LS4) and a bench-top analyzer (Biosen S-Line analyzer, BSL) during Double Poling. Blood samples were collected from the earlobe and fingertip immediately before exercise, at the end of each of five stages, and at 1-, 3-, 5-, and 7-min post-exercise. Forty healthy university students participated as volunteers. During the study, they performed double poling on a ski ergometer with progressively increasing load. Lactate levels were measured using both the BSL and LS4 analyzers. Fingertip Bla values were significantly higher than earlobe values, with a mean bias of -0.66mmol/L, reaching -0.86mmol/L in the 4-8mmol/L range. At the earlobe, the highest CCC between BSL and LS4-a was 0.84 (> 8mmol/L), and for BSL and LS4-b, it was 0.85 (> 8mmol/L). At the fingertip, the highest CCC between BSL and LS4-c was 0.68 (> 8mmol/L), and for BSL and LS4-d, it was 0.52 (> 8mmol/L). Comparing LS4-a and LS4-b at the earlobe, the highest CCC was 0.83 (0-4mmol/L). At the fingertip, comparing LS4-c and LS4-d, the highest CCC was 0.68 (> 8mmol/L). Blood lactate concentrations are higher at the fingertip than the earlobe during SkiErg double poling. The LS4 is less reliable, especially at the fingertip, so using the earlobe with the BSL analyzer is recommended for accurate measurements.

Consistency analysis of two fingertip capillary blood sampling methods for complete blood count

This study was performed to analyze fingertip capillary blood sampling in pediatric patients using microcapillary blood collection tubes and microhematocrit tubes and to compare the blood cell analysis results obtained via these two blood collection methods. Finger capillary blood was collected from 110 outpatients using microcapillary blood collection tubes and microhematocrit tubes and complete blood count analysis was performed with a Sysmex XS-900i hematology analyzer and manual microscopy for blood cell morphology. Paired data was evaluated for agreement and bias using the microhematocrit samples as the reference group and the samples from the microcapillary blood collection tubes as the observation group. The two blood collection methods demonstrated good agreement for measuring red blood cell (RBC) parameters (i.e., RBC, Hb, Hct, MCV, MCH and MCHC), wherein the relative bias was > allowable total error (TEa) in 0.91%, 1.82%, 11.82%, 1.82%, 0.91% and 8.18% of the parameter measures, respectively. According to industry requirements, the proportion of samples meeting the acceptable bias level should be > 80%. Additionally, the estimated biases at each medical decision level were within clinically acceptable levels for RBC, Hb, Hct, and MCV. However, the proportion of WBC and PLT counts with relative bias > TEa was 25.45% and 35.45%, respectively. Furthermore, the relative bias of the WBC count at the medical decision level of 0.5 × 109/L and that of the PLT counts at the medical decision levels of 10 × 109/L and 50 × 109/L were clinically significant. Bland–Altman analysis further showed a mean bias of 0.66 × 109/L (95% LoA, − 0.79 to 2.11) for the WBC count and 39 × 109/L (95% LoA, − 46 to 124) for the PLT count from the blood samples collected in the microcapillary blood collection tubes compared with the counts of those collected in the microhematocrit tubes. Neutrophil, monocyte, lymphocyte, eosinophil, and PLT counts increased significantly in the microcapillary blood collection tubes compared with those in the microhematocrit tubes, along with an elevated number of instrument false alarms (P < 0.05). The two capillary blood collection devices exhibit performance differences. Therefore, clinicians should pay attention to the variation in results caused by different blood collection methods.

Accuracy of FreeStyle Libre flash glucose monitoring in patients with type 2 diabetes who migrated from highlands to plains

BACKGROUND The FreeStyle Libre flash glucose monitoring (FGM) system entered the Chinese market in 2017 to complement the self-monitoring of blood glucose. Due to its increased usage in clinics, the number of studies investigating its accuracy has increased. However, its accuracy has not been investigated in highland popu-lations in China. AIM To evaluate measurements recorded using the FreeStyle Libre FGM system compared with capillary blood glucose measured using the enzyme electrode method in patients with type 2 diabetes (T2D) who had migrated within 3 mo from highlands to plains. METHODS Overall, 68 patients with T2D, selected from those who had recently migrated from highlands to plains (within 3 mo), were hospitalized at the Department of Endocrinology from August to October 2017 and underwent continuous glucose monitoring (CGM) with the FreeStyle Libre FGM system for 14 d. Throughout the study period, fingertip capillary blood glucose was measured daily using the enzyme electrode method (Super GL, China), and blood glucose levels were read from the scanning probe during fasting and 2 h after all three meals. Moreover, the time interval between reading the data from the scanning probe and collecting fingertip capillary blood was controlled to &lt; 5 min. The accuracy of the FGM system was evaluated according to the CGM guidelines. Subsequently, the factors influencing the mean absolute relative difference (MARD) of this system were analyzed by a multiple linear regression method. RESULTS Pearson’s correlation analysis showed that the fingertip and scanned glucose levels were positively correlated (R = 0.86, P = 0.00). The aggregated MARD of scanned glucose was 14.28 ± 13.40%. Parker's error analysis showed that 99.30% of the data pairs were located in areas A and B. According to the probe wear time of the FreeStyle Libre FGM system, MARD1 d and MARD2–14 d were 16.55% and 14.35%, respectively (t = 1.23, P = 0.22). Multiple stepwise regression analysis showed that MARD did not correlate with blood glucose when the largest amplitude of glycemic excursion (LAGE) was &lt; 5.80 mmol/L but negatively correlated with blood glucose when the LAGE was ≥ 5.80 mmol/L. CONCLUSION The FreeStyle Libre FGM system has good accuracy in patients with T2D who had recently migrated from highlands to plains. This system might be ideal for avoiding the effects of high hematocrit on blood glucose monitoring in populations that recently migrated to plains. MARD is mainly influenced by glucose levels and fluctuations, and the accuracy of the system is higher when the blood glucose fluctuation is small. In case of higher blood glucose level fluctuations, deviation in the scanned glucose levels is the highest at extremely low blood glucose levels.

Oral bacteriotherapy is associated with decreased blood lactate concentration during exercise at high altitude

Exposure to high altitude leads to decreased oxygen saturation (SpO2) and, in some individuals, the development of adverse outcomes such as acute high-altitude sickness. A further decrease in oxygen availability during exercise at altitude leads to greater anaerobic metabolism and increased lactate production. Previous studies show that ingestion of the probiotic SLAB51 (O2BOOSTER; Hecto, Seoul, South Korea) improved SpO2 in individuals exercising in a hypobaric chamber (simulated altitude 16,732 ft (~5100 m) above sea level) compared to individuals who did not ingest the probiotic. However, the use of SLAB51 during exercise at high terrestrial altitude has yet to be studied. We hypothesized that individuals who ingest SLAB51, relative to those given a placebo, will exhibit less of a difference in lactate production during sub-maximal exercise when paired high-altitude and sea-level values pre- and post-exercise are compared. We recruited 17 participants of Latino ancestry, ages 19-63 years, for studies at sea level (University of California, San Diego) and at high altitude (University of California White Mountain Barcroft Research Station, 3801 m). At sea level and on the third or fourth day at high altitude, we measured SpO2 and heart rate (HR) via pulse oximetry (forehead probe) before and during the last 30 seconds of a 10-minute submaximal (1 watt/kg) bout of exercise on a cycle ergometer (n = 5 for each group). Blood lactate concentration was measured before and after exercise from fingertip capillary blood samples. The respective differences between SpO2 and HR pre-exercise and during the final 30 seconds of exercise (the ΔSpO2 and ΔHR) were calculated for all individuals. The Δlactate blood concentration was calculated by the difference between lactate pre- and post-exercise. ΔSpO2, ΔHR, and Δlactate values were computed for sea level and high altitude. The difference between the respective sea-level Δ and the high-altitude Δ values were quantified for each measurement and compared between the groups who received either SLAB51 or the placebo upon arrival, and the same treatment for three days at high altitude. As expected, exercise increased HR and blood lactate concentration at sea level and high altitude and decreased SpO2 at high altitude. The difference of Δlactate between sea level and high altitude was lower (p = 0.023) in the SLAB51 (n = 4, 0.93 ± 0.18 mmol/L) versus placebo (n = 4, 1.63 ± 0.13 mmol/L) group. However, there were no significant differences of ΔSpO2 and ΔHR between groups. Our findings show that taking SLAB51 could lower the production of lactate during submaximal exercise at high altitude. This means that anaerobic glycolysis may play a smaller role in energy production when the probiotic is taken compared to the placebo. To further understand the influence of SLAB51 as a supplement on exercise performance at altitude, more research with a bigger sample size is necessary. Supported by LEA Altitude Performance Fund. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Prevalence of Anemia among Adult Male Camel Milk Consumers and Nonconsumers in Rural Rajasthan: A Community-based Cross-sectional Study

Introduction: Anemia is an important nutritional disorder with major public health importance. In India, anemia prevalence among adult males was 25.0% (NFHS-5, 2020–2021). Being rich in iron and other micronutrients camel milk might decrease the nutritional anemia prevalence. The objective was to determine the prevalence of anemia among adult male camel milk consumers (CMC) versus nonconsumers in rural Rajasthan. Materials and Methods: A community-based study was conducted among 30 CMC adult male. All the participants meeting the eligibility criteria were recruited. The same numbers of non-CMC (NCMC) adult males were recruited with respect to similar age, sex, and residence. After obtaining informed consent a semi-structured interview schedule containing sociodemographic, dietary characteristics were introduced to all participants in June 2013. The participants were assessed for their hemoglobin (Hb) level from fingertip capillary blood using the Cyanmet Hb method as suggested by Dacie and Lewis. The prevalence of anemia in each group was compared. Data were entered into Microsoft Excel version 2013 and analyzed in Stata version 13. Results: The prevalence of anemia was more (26.6%) among nonconsumers than CMCs (16.6%). There was a significant difference between these groups (P &lt; 0.001). The mean (standard deviation) Hb (g/dL) level of the male in the CMC and NCMC categories was found to be 13.0 (0.99) and 12.22 (1.51) in adult males, respectively. This difference was statistically significant (P = 0.021). Conclusion: The prevalence of anemia among CMC adult males was lower than nonconsumers with relatively higher levels of Hb in the former group. Future studies are recommended for further inference.

Factors Influencing Glucose Response to Exercise Following Oral Glucose Load

PURPOSE: Exercise can reduce postprandial hyperglycemia by increasing contraction-mediated glucose uptake in skeletal muscles. However, postprandial exercise does not always have similar effects in all patients. This study aimed to analyze the factors influencing glucose response to exercise following oral glucose load.METHODS: We enrolled 169 adults with impaired glucose tolerance (IGT, n = 64) and type 2 diabetes (T2D, n = 105). We measured fingertip capillary blood glucose level at 30-minute intervals over 2-hour after the 75-g oral glucose ingestion. In addition, a single bout of moderate-intensity aerobic exercise was conducted at 30 minutes after 75-g oral glucose ingestion. We also measured glycated hemoglobin (HbA1c), insulin, insulin resistance (HOMA-IR), β-cell function (HOMA-β), physical activity level questionnaire and anthropometrics.RESULTS: There was a significant difference in glucose concentration between the IGT and T2D groups (&lt;i&gt;p&lt;/i&gt; &lt;.001). Additionally, we found that patients with short diabetes duration (≤1 year) had significantly more decrease in glucose concentration than long diabetes duration (≥10 years) (&lt;i&gt;p&lt;/i&gt; &lt;.001). A regression analysis showed that waist circumference, visceral fat area, fasting glucose, HbA1c, and HOMA-IR affected glucose response to exercise following oral glucose load in patients with T2D.CONCLUSIONS: Higher volumes of exercise following an oral glucose load may be necessary for individuals with Type 2 Diabetes (T2D) who have a longer disease duration, larger waist circumference, increased visceral fat area, higher HbA1c levels, and elevated HOMA-IR values in order to prevent hyperglycemia.

Serum osmolality measured in fingertip capillary blood is comparable to serum osmolality measured in venous blood.

Blood osmolality is considered the gold standard hydration assessment, but has limited application for technical and invasive reasons. Paired antecubital-venous blood and fingertip-capillary blood were collected pre- and 30min post-drinking 600mL water in 55 male/female participants. No bias (0.2 mOsmo/kg, limits of agreement = -2.5 to 2.8 mOsmo/kg) was found between sampling methods, with high linear correlation (Spearman's r=0.95, P<0.001). Capillary blood sampling offers an accurate less-invasive method for determining serum osmolality than venous blood sampling.

Effects of Acute Exergame on Glucose Control After Glucose Ingestion in Individuals with Pre- and Type 2 Diabetes

OBJECTIVES The purpose of present study was to analyze the acute effects of exergame on glucose control after glucose ingestion in individuals with pre- and type 2 diabetes.METHODS We enrolled 60 adults with pre-diabetes (Pre-DM, 58.1±9.2 years, n=20) and type 2 diabetes (T2DM, 61.7±9.9 years, n=40). We measured fingertip capillary blood glucose level at 10 min before, 30 min after, 60 min after and 120 min after the 75-g oral glucose tolerance test (OGTT). For the non-exergame control days, participants pursued normal daily activities but refrained from unusual strenuous physical activity. On the exercise days, participants conducted on exergame at 30 min after 75-g OGTT for 30 min.RESULTS Acute exergame reduced the glucose level 60 min, ∆BG30-60 and ∆BG60-120 after glucose ingestion compared to the control trials (&lt;i&gt;p&lt;/i&gt;&lt;0.01, &lt;i&gt;p&lt;/i&gt;&lt;0.01 and &lt;i&gt;p&lt;/i&gt;&lt;0.001 respectively). The cumulative glucose total area under the curve was lower with exergame than under the control condition (&lt;i&gt;p&lt;/i&gt;&lt;0.05).CONCLUSION Acute exergame after glucose ingestion can improve hyperglycemia and glycemic excursion.

Doping control analysis of trimetazidine in dried blood spot.

Dried blood spot (DBS) analysis has been an inherent part of sports drug testing through the technological advancements of the past decade. Trimetazidine, a non-threshold banned substance, is excreted into urine after a dose of the permitted drug lomerizine. Therefore, a lomerizine-specific metabolite (M6) is analyzed to confirm the origin of trimetazidine in traditional urine analysis. Application studies were conducted to develop an analytical method for trimetazidine applicable to DBS. These studies comprise (1) the effect of different sampling sites on the detection of trimetazidine, (2) the determination of the appropriate trimetazidine level required for DBS analysis, and (3) differentiating between trimetazidine and lomerizine use. A high-resolution mass spectrometric method for detecting trimetazidine in DBS was validated. After oral administration of trimetazidine (n = 7), venous and capillary blood (fingertip and upper arm) were spotted on cellulose paper. Trimetazidine could be identified in DBS in all subjects up to 60 h after administration. The limit of detection was 0.05 ng/ml, and the limit of identification was 0.06 ng/ml, suggesting the minimum required performance level of 0.2 ng/ml. In the fingertip capillary blood, biases of 9.7% (vs. upper arm) and 13.0% (vs. vein) were observed in the trimetazidine intensity; however, there were no concerns in the qualitative analysis. After administering lomerizine (n = 10), the intact lomerizine has a strong peak intensity in blood compared to trimetazidine. Contrary to urine analysis, the M6 was less detectable in blood. Laboratories should confirm intact lomerizine whenever trimetazidine is identified in DBS.

Metabolic and Performance Responses to a Simulated Routine in Elite Artistic Swimmers.

The purpose of this investigation was to examine the interrelationship between time spent underwater (UW), movement frequency and accompanying blood acid base balance response. Elite artistic swimmers (n = 6) participated in the investigation and were all familiar with the testing procedures. All athletes completed the same choreographed artistic swimming routine. The routine was videoed and the number of movements during each 'lap' of the routine counted. Fingertip capillary blood samples were collected prior to the routine 60 sec post routine for pH, partial pressure of carbon dioxide (pCO2), partial pressure of oxygen (pO2), bicarbonate (HCO3-) and potassium (K+). and lactate (La) concentration (mmol/L). On a separate day an incremental exercise test to exhaustion was performed on a cycle ergometer for determination of maximal oxygen uptake (VO2max). Over half the routine was performed underwater (56 ± 4%). Aside from pCO2 (-1.07 ± 12.29%, p = 0.686), there were significant changes in all variables measured from the BG analysis. VO2peak was significantly correlated to total UW of the routine (r = -0.93; p = 0.007). as well as ∆PO2 r = 0.47 and ∆HCO3 r = 0.51. There was also a significant correlation between total UW and post routine pCO2 (r = 0.86; p = 0.030). There was also a significant correlation between total movements during the routine and post pO2 (r = -0.83; p = 0.044). These data show UW in combination with movement rate during a AS routine imfluence the metabolic response to the exercise. In addition, VO2max represents an important performance variable influencing AS performance.

Diabetes Complications and Related Comorbidities Impair the Accuracy of FreeStyle Libre, a Flash Continuous Glucose Monitoring System, in Patients with Type 2 Diabetes.

Although flash continuous glucose monitoring systems (FCGM) accuracy has been extensively studied in diabetes, its accuracy is still not fully evaluated in type 2 diabetes (T2D) patients in real-world settings. In the present study, we aim to assess the effects of diabetes complications and related comorbidities on FCGM accuracy in T2D patients with diabetes complications and related comorbidities in the real world. FCGM data were collected at eight-time points daily (3 AM, 7 AM, 9 AM, 11 AM, 1 PM, 5 PM, 7 PM, and 9 PM) from 742 patients with T2D and compared with simultaneous fingertip capillary blood glucose (reference blood glucose, REF), and the difference was evaluated using Parkes error grid (PEG), surveillance error grid (SEG), and logistic regression analysis. In total, 25,579 FCGM/REF data pairs were included in the study. The FCGM values were lower than the paired REF values in 75% of the pairs. The maximum bias (-23.0%) and maximum mean absolute relative difference (24.5%) were observed at 3 AM among eight-time points. SEG analysis also demonstrated the highest percentage of paired readings in moderate and great risk zone (C and D) at 3 AM than PEG analysis (7.33% vs 0.43%, P<0.001). According to the SEG classification, hypoglycemia, infection, diabetic foot, diabetic ketoacidosis, and hypertension were independent risk factors that impaired FCGM accuracy in patients. FCGM commonly underestimates blood glucose levels. Compared with PEG, SEG analysis seems more conducive to the analysis of FCGM performance. The present data highlights the impairment of diabetes complications and related comorbidities on the FCGM accuracy in T2D patients.

Distribution of Oxygen Partial Pressures in Native Capillary Fingertip Blood Samples From a Large Diabetes Patient Population.

A data set comprising 28 533 oxygen partial pressure (pO2) values gathered from nonarterialized fingertip capillary blood samples of a large diabetes population is reported. Data were gathered routinely as part of the ongoing clinical surveillance activities of a blood glucose monitoring (BGM) system manufacturer. Overall, a mean pO2 level of 75.3 mm Hg was recorded, with a standard deviation (SD) of 10.6 mm Hg and a range of 27.7 to 151.5 mm Hg. This data set, unique in terms of size, gathered more than 14 years at four separate clinical sites, provides a robust and comprehensive representation of an unmanipulated pO2 fingertip capillary sample distribution, which should be of interest for physiological reasons, but also from a medical device development perspective, in which the effect of pO2 on device performance may be an important design consideration.

Clinical interpretation of the flash continuous glucose monitoring data out of range in type 2 diabetes: A real-world study

AimsFlash continuous glucose monitoring system (FCGM) reports nocturnal hypoglycemia with low accuracy in low blood glucose. In our study, we aimed to evaluate the accuracy and clinic profile of FCGM data out of range in T2DM. MethodsFCGM data out of range were measured in T2DM patients at eight-time points of a day and compared with fingertip capillary blood glucose levels (REFs) as reference values. ResultsA total of 307 out-of-range cases from 25,886 paired FCGM/REF data from 742 T2DM patients were evaluated. The distribution of “LO” and “HI” cases displayed the significant difference between eight-time points (P < 0.001) with the highest frequency (6.52%) of the “LO” value at 3 AM. The consistency rate between FCGM “LO” readings and REF < 40 mg/dl was far lower than that of FCGM “HI” readings with REF > 500 mg/dl (0.84% vs. 52.2%). In addition, the frequency of some clinical characteristics, including diabetes duration, hypoglycemia, hypertension, HbA1c, and Hb, were higher in patients with FCGM “LO” readings compared to those with “HI” readings. ConclusionsThese findings suggest the results and treatment regarding FCGM “LO” data should be interpreted with strong caution in the light of the emerging possible adverse measurement in patients.

Non‐Invasive Sweat‐Based Tracking of L‐Dopa Pharmacokinetic Profiles Following an Oral Tablet Administration

AbstractLevodopa (L‐Dopa) is the “gold‐standard” medication for symptomatic therapy of Parkinson disease (PD). However, L‐Dopa long‐term use is associated with the development of motor and non‐motor complications, primarily due to its fluctuating plasma levels in combination with the disease progression. Herein, we present the first example of individualized therapeutic drug monitoring for subjects upon intake of standard L‐Dopa oral pill, centered on dynamic tracking of the drug concentration in naturally secreted fingertip sweat. The touch‐based non‐invasive detection method relies on instantaneous collection of fingertip sweat on a highly permeable hydrogel that transports the sweat to a biocatalytic tyrosinase‐modified electrode, where sweat L‐Dopa is measured by reduction of the dopaquinone enzymatic product. Personalized dose‐response relationship is demonstrated within a group of human subjects, along with close pharmacokinetic correlation between the finger touch‐based fingertip sweat and capillary blood samples.

Non-Invasive Sweat-Based Tracking of L-Dopa Pharmacokinetic Profiles Following an Oral Tablet Administration.

Levodopa (L-Dopa) is the "gold-standard" medication for symptomatic therapy of Parkinson disease (PD). However, L-Dopa long-term use is associated with the development of motor and non-motor complications, primarily due to its fluctuating plasma levels in combination with the disease progression. Herein, we present the first example of individualized therapeutic drug monitoring for subjects upon intake of standard L-Dopa oral pill, centered on dynamic tracking of the drug concentration in naturally secreted fingertip sweat. The touch-based non-invasive detection method relies on instantaneous collection of fingertip sweat on a highly permeable hydrogel that transports the sweat to a biocatalytic tyrosinase-modified electrode, where sweat L-Dopa is measured by reduction of the dopaquinone enzymatic product. Personalized dose-response relationship is demonstrated within a group of human subjects, along with close pharmacokinetic correlation between the finger touch-based fingertip sweat and capillary blood samples.

Differences in circulating fatty acid-binding protein 4 concentration in the venous and capillary blood immediately after acute exercise

BackgroundCirculating fatty acid-binding protein 4 (FABP4) is a marker for various diseases. It would be highly useful to have simple and less invasive techniques for the assessment of FABP4 concentrations in the clinical research setting. The purpose of the present study was to assess the concordance of circulating FABP4 concentrations in venous and capillary blood both at rest and immediately after acute exercise in healthy young males.ResultsThirty-eight healthy young male adults aged from 19 to 25 years (mean age, 20.8 ± 1.2 years) were recruited. Paired blood samples were taken from the cubital vein (venous) and fingertip (capillary) blood at rest (resting state) and immediately after incremental exercise (exercising state). Blood samples were analyzed to determine the circulating FABP4 concentration using an enzyme-linked immunosorbent assay. Pearson’s correlation coefficients for circulating FABP4 concentrations between venous and capillary blood samples indicated a strong positive correlation in both the resting and exercising state (resting state: r = 0.982, exercising state: r = 0.989, both p < 0.001). The mean FABP4 concentration was similar between venous and capillary blood in the resting state (p = 0.178), whereas it was significantly higher in capillary blood than in venous blood in the exercising state (p < 0.001). Furthermore, Bland–Altman plots showed a non-significant bias (− 0.07 ± 0.61 ng/mL, p = 0.453) in the resting state, whereas a significant bias (− 0.45 ± 0.61 ng/mL, p < 0.001) was observed in the exercising state.ConclusionsThese results indicate that capillary blood sampling can slightly overestimate circulating FABP4 concentrations under a physiologically dynamic state. However, the association between the venous and capillary blood in terms of FABP4 concentration was very strong, suggesting that capillary blood sampling can detect changes in FABP4 concentration in both physiologically steady and dynamic states.

The Correlation between Time in Range and Diabetic Microvascular Complications Utilizing Information Management Platform.

Background In recent years, the time of blood glucose within the target range is a new research hotspot in blood glucose management. TIR is expected to be a novel indicator for evaluating the efficacy of glycemic control and predicting diabetic complications. However, its relationship with diabetic complications has not been fully elucidated. Objective To explore the relationship between time in range (TIR) and glycosylated hemoglobin (HbA1C) through the information big data management platform. Possible association between TIR and diabetic microvascular complications (retinopathy, nephropathy, and neuropathy) was investigated, attempting to provide theoretical basis for the clinical application of TIR and to explore the TIR control scope suitable for diabetic patients. Methods A total of 5,644 type 2 diabetic patients hospitalized in the Department of Endocrinology, the Second Affiliated Hospital of Nanchang University, were selected from April 2017 to June 2020. Fingertip capillary blood glucose monitoring (FCGM) was monitored for a total of 455,664 times, and patients who are nondiabetic, pregnant, or with diabetic ketosis were excluded. Patients with 7 blood glucose points monitored for at least three consecutive days were selected as subjects in the study. 1,895 males and 1,513 females with diabetes were included, with an average age of (59.74 ± 13.40) years old and an average course of disease of 8.28 ± 7.11 years. The proportion of time in range (TIR) (70∼180 mg/dl) within the target range and the correlation between TIR and HbA1C were analyzed, as well as the relationship between TIR and the risk of diabetic complications. Results (1) The average of TIR and HbA1C was 49.65 ± 23.36% and 8.92 ± 2.49%, respectively, and was linearly correlated. With the decrease of TIR, HbA1C increased significantly, and the difference was statistically significant (P < 0.01, R2 = 0.458). The correlation coefficient of mean TIR with mean HbA1C was −0.626. (2) There were 836 patients diagnosed with diabetic nephropathy (DN). The difference of TIR value between DN and non-DN was significant (T = 2.250, P < 0.05). Risk assessment showed the lower the TIR was, the higher the risk of DN was. TIR less than 40% was a risk factor for DN (OR = 1.249, 95% CI: 0.915–1.375). (3) There were 1,296 patients diagnosed with diabetic peripheral neuropathy (DPN). The difference of TIR value between DPN and non-DPN was significant (T = 3.844, P < 0.01). TIR value less than 70% was a risk factor for DPN (OR = 1.030, 95% CI: 0.769–1.379). (4) There were 2,077 patients diagnosed with diabetic retinopathy (DR). The difference of TIR value between DPN and non-DPN was significant (T = 3.608, P < 0.01). TIR value less than 50% was a risk factor for DR (OR = 1.092, 95% CI: 0.898–1.264). Summary. TIR may serve as a reference index for short-term blood glucose control, strongly reflecting the clinical blood glucose regulation and predicting the risk of diabetic microvascular complications.

Effect of Simulated Matches on Post-Exercise Biochemical Parameters in Women's Indoor and Beach Handball.

This study assesses the status of hydration and the acid-base balance in female handball players in the Polish Second League before and after simulated matches in both indoor (hall) and beach (outdoor) conditions. The values of biochemical indicators useful for describing water-electrolyte management, such as osmolality, hematocrit, aldosterone, sodium, potassium, calcium, chloride and magnesium, were determined in the players’ fingertip capillary blood. Furthermore, the blood parameters of the acid-base balance were analysed, including pH, standard base excess, lactate and bicarbonate ion concentration. Additionally, the pH and specific gravity of the players’ urine were determined. The level of significance was set at p < 0.05. It was found that both indoor and beach simulated matches caused post-exercise changes in the biochemical profiles of the players’ blood and urine in terms of water-electrolyte and acid-base balance. Interestingly, the location of a simulated match (indoors vs. beach) had a statistically significant effect on only two of the parameters measured post-exercise: concentration of calcium ions (lower indoors) and urine pH (lower on the beach). A single simulated game, regardless of its location, directly affected the acid-base balance and, to a smaller extent, the water-electrolyte balance, depending mostly on the time spent physically active during the match.

Evaluation of the Abbot FreeStyle Optium Neo H blood glucose meter in the hyperbaric oxygen environment.

This study investigated the effects of hyperbaric oxygen treatment (HBOT) on the accuracy and reliability of point-of-care fingertip capillary blood glucose values in euglycaemic non-diabetic participants compared against venous serum blood glucose samples processed in an accredited pathology laboratory. Ten non-diabetic hyperbaric staff members (age 35-55 years) underwent a standard 243 kPa HBOT exposure for 95 minutes. Blood glucose levels were measured via (i) finger-prick capillary test using the FreeStyle Optium™ Neo H glucometer and (ii) venous serum test using the Cobas 6000 laboratory analyser. Samples were taken at (T1) 0 minutes (pre-HBOT), (T2) 25 minutes, and (T3) 55 minutes into HBOT. All participants were euglycaemic at T1 (BGL 3.8-5.4 mmol·L⁻¹). The highest venous serum value was 5.90 mmol·L⁻¹ at T3 and the highest capillary value was 6.30 mmol·L⁻¹ at T1. Post hoc tests showed a statistically significant difference between the mean capillary result pre-dive (T1) and readings at T2 (P = 0.001) and T3 (P < 0.001) while differences between T2 and T3 capillary results were not statistically significant, illustrating the effect of HBOT on capillary beds. Differences in venous values across the time points were not significant. Venous serum glucose samples processed in an accredited laboratory may be more consistently accurate, but capillary point-of-care testing avoids delays in sample processing and provides glucose data that are of clinical relevance. The FreeStyle Optium™ Neo H glucometer is safe to use and provides a reliable measurement of blood glucose in the HBOT environment.

Comparison of plasma fatty acid binding protein 4 concentration in venous and capillary blood.

Circulating fatty acid binding protein 4 (FABP4) is associated with various diseases and simple and less invasive techniques for assessment of FABP4 concentration are required in clinical research setting. The purpose of the present study was to assess the correlation of plasma FABP4 concentration between venous and capillary blood in healthy young adults. Twenty-eight healthy young adults aged from 20 to 26 years (mean age, 22.2 ± 1.4 years, 14 males and 14 females) were included. Paired resting blood samples were taken from the cubital vein (venous) and fingertip (capillary) blood. Plasma FABP4 concentration in both blood was analyzed by enzyme-linked Immunosorbent assay. Plasma FABP4 concentration did not differ significantly between venous and capillary blood (−0.11± 0.75 ng/mL, p = 0.447, 95%CI: -0.402–0.182). Pearson’s correlation coefficient for plasma FABP4 concentration between venous and capillary blood samples suggests strong correlation (r = 0.961, p < 0.001). The Bland & Altman plot showed a non-significant bias (−0.11 ± 0.75 ng/mL, p = 0.684) and the 95% limits of agreement ranged from −1.59 to 1.37 ng/mL. FABP4 concentration in both venous and capillary blood was significantly higher in females than in males (venous blood: p = 0.041; capillary blood: p = 0.049). These results suggest that capillary blood sampling can detect gender difference and is useful for the assessment of FABP4 concentration.