Elathy Mathirai is a Siddha herbomineral formulation indicated to cure sori(itching), sirangu(Scabies), suram(fever), vaayu, maandham(gastro intestinal disorder) . The aim of the study is to standardize Elathy Mathirai to ensure its quality, purity and safety through its physicochemical, chromatographic, phytochemical, heavy metal, pesticide residue and aflatoxins analysis and the secondary objective is to evaluate its antimicrobial property. This study determines the physicochemical paramaters that are loss on drying, total ash, acid insoluble ash, water soluble extractive, alcohol soluble extractive and pH as 5.667% , 5%, 0.014 %, 14.93 %, 8.3% and 5.6 respectively denotes purity and quality of the drug. From the study results it is ensured that the test drug Elathy mathirai is free from pesticide toxicity and aflatoxins. Qualitative preliminary phytochemical analysis shows the presence of alkaloids, Flavanoids,steroids, triterpenoids, saponins, coumarins, phenol, tannin, sugar and betacyanin. HPTLC finger printing analysis reveals the presence of seven prominent peaks Rf value ranges from 0.04 to 0.39 corresponds to the presence of seven components present within it. It was observed from the results of In vitro anti microbial assay that the formulation EM reveals significant level of activity against bacteria Klebsiella pneumonia and observed moderate activity against Staphylococcal aureus, Pseudomonas aeruginosa and posess consistent anti fungal activity against Candida albicans and there is no significant effect on Aspergillus niger. The obtained results shows that the EM complies with the regulatory standard and also possess significant anti microbial property against the tested microbes.