To promote the application of recycled fine aggregates, fracture characteristics of concrete beams containing manufactured sand (MS) and recycled fine aggregate (RFA) were investigated. The fracture properties and acoustic emission (AE) characteristics of manufactured sand recycled fine aggregate concrete (MSRFAC) with 0 %, 25 %, 50 %, 75 % and 100 % RFA replacement rates were evaluated by three-point bending test of single-sided notched beams. The fracture mechanisms of MSRFAC beams were explored using AE and digital image correlation (DIC) techniques, and fracture process zone (FPZ) was qualitatively and quantitatively analyzed. The results indicated that the critical nominal stiffness, initial fracture toughness, fracture energy and characteristic length of MSRFAC decreased significantly with the increase of RFA replacement rate, up to 20.2 %, 37.7 %, 22.8 %, and 26.6 %, respectively, while the unstable fracture toughness was insensitive to the variation of RFA. Moreover, the AE activity, the tensile crack, large-scale crack, high amplitude, and high peak frequency in MSRFAC increased with increasing RFA replacement rate. According to the FPZ characteristics of MSRFAC, its FPZ length increased with the increase of RFA replacement rate, while the FPZ width decreased with the increase of RFA replacement rate. The critical FPZ lengths of MSRFAC with different RFA replacement rates were about 20.2–30.0 mm, and the maximum FPZ widths were about 26.0–36.7 mm. The RFA weakened the friction mechanism and interlocking effect between aggregates of MSRFAC, resulting in a faster FPZ propagation rate and smaller damage width of MSRFAC than normal manufactured sand concrete.