Fluid Filtration Research Articles

Deep plutonic bodies over low-frequency earthquakes revealed from receiver-side Green's functions

Seismological heterogeneity in subduction zones provides insights into slow earthquakes and potential megathrust earthquakes. Studies at the Kii Peninsula in the Nankai subduction zone suggest that there are high-density and high-velocity plutonic bodies in the accretionary prism over the subducting slab, potentially influencing megathrust earthquakes. The lateral variation of heterogeneity and the spatial extent of plutonic bodies remain to be investigated well. Our passive-source imaging of receiver-side Green's functions, from widely distributed campaign seismic observations, reveals a sharp negative S-wave velocity contrast on the top surface of the subducting Philippine Sea plate common to all along-dip profiles and a positive phase tilted upward in the forearc crust. The low permeability of the forearc crust prevents the infiltration of slab-dehydrated fluid further into the upper crust. In the western area, we also found positive phases tilted upward in the forearc crust. The negative phase extends towards the deeper extent of slow-earthquake sources. Meanwhile, the positive phase likely represents the top surface of plutonic rocks of the Kumano and Ohmine plutons that span all the way down to the plate interface. Together with observations of gravity anomaly, intraslab seismicity, and seismic tomography, our interpretation supports the presence of plutonic bodies which extend deep beneath the forearc crust as well as laterally over the subducting PHS slab, rather than a serpentinized mantle wedge. The upper plate is generally low in permeability, but areas with localized high permeability may exist on the updip side of tremor sources. This condition, wherein fluid can infiltrate upwards locally, may maintain the relatively less active slow earthquakes in the western area. The lateral variation of the upper-plate lithology likely influences fluid processes and slow earthquake activities.

An In Vitro Platform for Pharmacokinetic Quantification and Optimization of Cerebrospinal Fluid Filtration and Drug Circulation

Abstract Background: Modification of cerebrospinal fluid (CSF) transport dynamics is an expanding method for treating central nervous system injury and diseases. One application of this route is to modify the distribution of solutes in the CSF, however few tools currently exist for this purpose. The present study describes the use of a subject-specific in-vitro CSF phantom to perform a parametric evaluation of the Neurapheresis? CSF Management System (NP) for both CSF filtration and intrathecal drug circulation. Methods: An in-vitro CSF phantom was constructed which included realistic anatomy for the complete subarachnoid space. This platform was configured to test multiple parametric modifications of a dual-lumen catheter and filtration system. Calibrated mapping of tracer distribution and area under the curve (AUC) measurements were used to compare filtration and intrathecal-circulation schemes using the NP device versus the clinical standards of care. Results: The NP device showed potential advantages over lumbar drain (LD) for clearance of simulated subarachnoid hemorrhage, especially in the spinal canal. Use of the NP device in combination with simulated intracerebroventricular drug infusion (ICV) resulted in an increased extent and uniformity of tracer spread compared to ICV alone. Conclusion: NP improved clearance of simulated subarachnoid hemorrhage compared to LD and increased uniformity of tracer concentration via simulated ICV, providing support for NP use in these scenarios. The in-vitro CSF phantom system presented here quantitatively described the effects of parametric boundary modification on solute distribution in the intrathecal space.

Research and Evaluation of a Nanometer Plugging Agent for Shale Gas Horizontal Wells.

Due to the low permeability of shales, drilling fluid filtrate is very likely to intrude into the formation along the nanomicron pore joints of shale, leading to microfracture expansion and causing a well wall destabilization phenomenon. Based on the characteristics of the formation shale, a new nano plugging agent, styrene (St)/2-acrylamido-2-methylpropanesulfonic acid (AMPS)/ethyl acrylate (EA), was synthesized by emulsion polymerization using St, EA, and AMPS as reaction monomers. The analysis results using infrared spectroscopy and transmission electron microscopy showed that the product met the expected design. The particle size of the nano plugging agent St/AMPS/EA was mainly concentrated in the range of 50-130 nm. Thermogravimetric analysis revealed that the initial thermal decomposition temperature of St/AMPS/EA is 363.8 °C, indicating strong thermal stability. At a 3% concentration, the permeability of the mud cake decreased by 87.35%, and the maximum differential stress of the shale increased from 141.7 to 174.4 MPa. Stability analysis showed that the plugging agent maintained good plugging performance under various pH values and salinity conditions with fluid loss consistently around 3.2 mm. This nano plugging agent significantly improves wellbore stability, making it suitable for drilling in the Longmaxi Formation shale with developed microfractures, and provides valuable insights for similar formations.

Clay Minerals and Continental‐Scale Remagnetization: A Case Study of South American Neoproterozoic Carbonates

AbstractThe Neoproterozoic carbonate rocks of the Araras Group (Amazon Craton) and the Sete‐Lagoas and Salitre Formations (São Francisco Craton) share a statistically indistinguishable single‐polarity (reversed) characteristic direction. This direction is associated with paleomagnetic poles that do not align with the expected directions for primary detrital remanence. We employ a combination of classical rock magnetic properties and micro imaging/chemical analysis (in thin sections) using synchrotron radiation to examine these remagnetized carbonate rocks. Magnetic data indicate that most samples lack the anomalous hysteresis properties typically associated with carbonate remagnetization (except for distorted loops). Through a combination of Scanning Electron Microscopy with Energy Dispersive X‐ray Spectroscopy (SEM‐EDS), X‐ray Fluorescence (XRF), and X‐ray Absorption Spectroscopy (XAS), we identified subhedral/anhedral magnetite, or spherical grains with a core‐shell structure of magnetite surrounded by maghemite. These grains are within the pseudo‐single domain size range, as do most of the iron sulfides, and are spatially associated with potassium‐bearing aluminosilicates. While fluid percolation and organic matter maturation play a role, smectite‐illitization appears to be crucial for the growth of these phases. X‐ray diffraction analysis, in addition, identifies these silicates as predominantly highly crystalline illite, suggesting exposure to epizone temperatures. These temperatures were likely reached during the final stages of the Gondwana assembly (Cambrian), but remanence was only locked in afterward, in successive cooling events during the Early Middle Ordovician. This is supported by the carbonates' paleomagnetic pole positions compared to Gondwana's apparent polar wander path, and the absence of reversals, contrasting with the high reversal frequency of the Late Ediacaran/Cambrian.

Localization of albumin with correlative super resolution light- and electron microscopy in the kidney

The functioning of vertebrate life relies on renal filtration of surplus fluid and elimination of low-molecular-weight waste products, while keeping serum proteins in the blood. In disease, however, there is leak of serum proteins and tracing them to identify the leaking position within tissue with a nanometer resolution poses a significant challenge. Correlative microscopy integrates the specificity of fluorescent protein labeling into high-resolution electron micrographs. Using chemical tagging of albumin with synthetic fluorophores we achieve protein-specific labeling that preserve their post-embedding fluorescence after high-pressure freezing and freeze-substitution of murine kidney tissue. Using advanced registration techniques for super-resolution correlative light and electron microscopy, we can localize the labeled albumin with a high precision in the x-y plane of electron micrographs and cartograph its distribution. Thereby we can quantify the albumin concentration and measure a linear reduction gradient across the kidney filtration barrier. Our study shows the feasibility of combining different microscopy contrasts for tracing fluorescently labeled protein markers with super resolution in various tissue samples and opens new perspectives for correlative imaging in volume electron microscopy.

Early Precambrian eclogites in the Belomorian Province, eastern Fennoscandian Shield

This study focuses on a boudin of Archean amphibolite that occurs in the Gridino eclogite-bearing mélange (Belomorian Province, eastern Fennoscandian Shield) and contains two varieties of eclogite. Eclogite-1 is banded, retrogressed (omphacite is totally replaced by clinopyroxene-plagioclase symplectite), and deformed by recumbent tight folds identical to those in the TTG-amphibolite host. Zircon groups are dated at 2721 ± 26 Ma (Zrn-I, fir-tree zoning, no or small negative Eu anomalies, flat HREE patterns, 700–730 °C, 14–15 kbar), ca. 2.70 Ga (Zrn-II, rims around Zrn-I, grains, 750–900 °C, 11–14 kbar, high-P granulite facies), and ca. 2.70 Ga (Zrn-III, rims around Zrn-II). Newly formed Paleoproterozoic zircon crystals are lacking. Retrogressed eclogite-1 in which amphibolization and epidotization totally obliterated garnet and plagioclase-clinopyroxene symplectites is intruded by 2646 ± 46 Ma old granodiorite. Eclogite-2 is massive, contains preserved omphacite-garnet-quartz-rutile assemblages, and comprises two patches whose boundaries truncate folds in eclogite-1. A Paleoproterozoic age of eclogite-2 is evidenced by previously published 1.90 Ga ages of zircon rims around Archean zircon and newly formed crystals with omphacite inclusions. The largest patch contains tabular enclaves of three varieties of amphibolites. One variety is identical to some bands of totally retrogressed eclogite-1 changed into monomineral amphibolites in both the Neoarchean and Paleoproterozoic. The orientation and attitude of these tabular enclaves are coherent with those of the banding on limbs of recumbent tight folds in eclogite-1 within the boudin and later drag folds at its margins and form one and the same structural carcass or skeleton. These data along with recent findings of inherited 2.68 Ga old zircon with inclusions of omphacite in eclogite-2 indicate that this eclogite developed after Neoarchean eclogite-1 due to fluid infiltration along reactivated Neoarchean shear zones enveloping the boudin. These results favor the idea that the transition to the modern-style plate tectonics started not later than in the Neoarchean.

Chemistry and Physics of Wet Foam Stability for Porous Ceramics: A Review

The unique structural properties of porous ceramics, such as low thermal conductivity, high surface area, controlled permeability, and low density, make this material valuable for a wide range of applications. Its uses include insulation, catalyst carriers, filters, bio-scaffolds for tissue engineering, and composite manufacturing. However, existing processing methods for porous ceramics, namely replica techniques and sacrificial templates, are complex, release harmful gases, have limited microstructure control, and are expensive. In contrast, the direct foaming method offers a simple and cost-effective approach. By modifying the surface chemistry of ceramic particles in a colloidal suspension, the hydrophilic particles are transformed into hydrophobic ones using surfactants. This method produces porous ceramics with interconnected pores, creating a hierarchical structure that is suitable for applications like nano-filters. This review emphasizes the importance of interconnected porosity in developing advanced ceramic materials with tailored properties for various applications. Interconnected pores play a vital role in facilitating mass transport, improving mechanical properties, and enabling fluid or gas infiltration. This level of porosity control allows for the customization of ceramic materials for specific purposes, including filtration, catalysis, energy storage, and biomaterials.

Fluid-Infiltrated Metalens-Driven Reconfigurable Intelligent Surfaces for Optical Wireless Communications.

A reconfigurable intelligent surface (RIS), a leading-edge technology, represents a new paradigm for adaptive control of electromagnetic waves between a source and a user. While RIS technology has proven effective in manipulating radio frequency waves using passive elements such as diodes and MEMS, its application in the optical domain is challenging. The main difficulty lies in meeting key performance indicators, with the most critical being accurate and self-adjusting positioning. This work presents an alternative RIS design methodology driven by an all-silicon structure and fluid infiltration, to achieve real-time control of focal length toward a designated user, thereby enabling secure data transmission. To validate the concept, both numerical simulations and experimental investigations of the RIS design methodology are conducted to demonstrate the performance of fluid-infiltrated metalens-driven RIS for this application. When combined with different fluids, the resulting ultra-compact RIS exhibits exceptional varifocal abilities, ranging from 0.4 to 0.5 mm, thereby confirming the adaptive tuning capabilities of the design. This may significantly enhance the modulation of optical waves and promote the development of RIS-based applications in wireless communications and secure data-transmission integrated photonic devices.

Li enrichment in peridotites and chromitites tracks mantle-crust interaction

The ultramafic massifs of the Serranía de Ronda in southern Spain are the Earth's largest exposures of subcontinental lithospheric mantle (SCLM) peridotites (∼450 km2). These ultramafic massifs experienced asthenosphere melt percolation during their crustal emplacement. Mixing of these mafic melts with anatectic melts and fluids led to the formation of a world's unique Ni-arsenide-rich chromitite ores (hereafter CrNi ores) associated with orthopyroxenite and/or cordieritite (i.e., > 90 % volume of cordierite) hosted within the peridotites. This study uses laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to investigate the Li in rock-forming minerals of peridotite and CrNi ores to evaluate the role of Li as crustal tracer. Clinopyroxene crystallized from asthenospheric melts exhibits high Li contents (up to 8.5 ppm), exceeding the average values of the upper mantle (∼ 0.7 ppm), whereas orthopyroxene, olivine, and Cr-spinel from peridotite are mostly Li-depleted. In contrast, all rock-forming minerals of CrNi ores have abnormally high Li contents, displaying an overall Li enrichment trend toward the external parts of ultramafic massifs, on the way to the crustal rocks. This trend is evident in Cr-spinel from the CrNi ores, which display 6.9–7.9 ppm Li in the deepest portions of the massif (Arroyo de la Cala CrNi ore) up to 1.4–8.5 ppm in the shallowest part (La Gallega CrNi ore), as well as in orthopyroxenes that have 31.3–44.7 ppm Li in Arroyo de la Cala, and 45.1–51.4 ppm Li in La Gallega. Cordierite is present only in the CrNi ores situated in the external part of the ultramafic massifs, exhibiting 113.15–160.82 ppm Li in the Barranco de las Acedías CrNi ore and 36.5–60.5 ppm Li in La Gallega CrNi ore. Similarly to Li, LREE, fluid-mobile elements (K, Rb, Ba), and Sr in orthopyroxenes from the CrNi ores display enrichment from the inner to the outer parts of the ultramafic massif. These geochemical variations suggest that Li enrichment in CrNi ores and host peridotites was a twofold process: (1) asthenospheric melt percolation slightly increased Li abundances in the SCLM peridotites by modal and cryptic metasomatism involving clinopyroxene; (2) additional infiltration of Li-bearing crustally-derived fluids during the intracrustal emplacement of the mantle section boosted the Li contents of minerals in the CrNi ores. Our results highlight that Li may effectively track the interaction of the SCLM with crustal components.

Ca–Zn isotope fractionation during fluid–rock interaction in subduction zones and its implication for deep carbon cycle

Subducting slabs transport Earth's surface carbon into the deep mantle, with a portion of this carbon potentially recycled through volcanic eruptions at mid-ocean ridges or ocean islands. Non-traditional stable isotopes such as calcium (Ca) and zinc (Zn) are increasingly used to trace this deep carbon cycle. However, the potential for Ca and Zn isotope fractionation in the subduction zone, critical for the successful application of these tracers, remains insufficiently explored. In this study, we investigate high-pressure carbonated metamafic rocks and enclosed vein systems from a Paleo-oceanic subduction complex (SW Tianshan) to explore the possible fractionation of Ca and Zn isotope during subduction-related metamorphism and fluid–rock interaction. The δ44/40Ca (0.77 ‰–0.90 ‰) and δ66Zn isotope compositions (0.20 ‰–0.39 ‰) of greenschist-, blueschist- and eclogite-facies metabasites are consistent with their protoliths (OIBs and MORBs). We observe no systematic fractionation of either Ca and Zn isotopes across prograde metamorphism and associated dehydration. However, the precipitation of carbonate in surrounding rock along fluid pathways leads to an increase in the δ44/40Ca of residual fluids (>0.90 ‰). More significantly, we find detectable Zn isotope fractionation (ΔA-H = ẟ66Znaltered - ẟ66Znhost of 0.06–0.10 ‰) during fluid-mediated metasomatism of some blueschists/eclogites. Light Zn isotopes are preferentially released into the infiltrating fluid, resulting in an elevated δ66Zn isotope composition in the immediate carbonated eclogites along the vein. The modified slab fluids during migration are likely charactered by heavy δ44/40Ca and light δ66Zn compositions, which may play a crucial role in determining the low δ66Zn values found in some arc lavas. However, the MORB-like δ44/40Ca compositions observed in global arc magmatic rocks suggest that the slab-released Ca (possibly along with some carbon) is probably sequestered and stored along the migration path of the fluid, thereby hindering the slab–arc carbon recycling. Our study emphasizes that intraslab fluid–rock interaction results in similar δ44/40Ca and δ66Zn signature in carbonate-bearing eclogites compared to sedimentary carbonates. Consequently, deeply subducted carbonate-bearing eclogites –not necessarily sedimentary carbonates– might carry light δ44/40Ca and heavy δ66Zn signatures into the deep mantle, potentially influencing the corresponding Ca-Zn isotope compositions of some mantle-derived basalts.

Shear-induced fluid localization, episodic fluid release and porosity wave in deformable low-permeability rock salt

Understanding fluid distribution and migration in deformable low-permeability rock salt is critical for geologic disposal of nuclear waste. Field observations indicate that fluids in a salt formation are likely compartmentalized into relatively isolated patches and fluid release from such a formation is generally episodic. The underlying mechanism for these phenomena remains poorly understood. In this paper, a hydrological-mechanical model is formulated for fluid percolation in a rock salt formation under a deviatoric stress. Using a linear stability analysis, we show that a porosity wave (a train of alternating high and low porosity pockets) can emerge from positive feedbacks among intergranular wetting, grain boundary weakening and shear-induced material dilatancy. Fluid localization or episodic release can be viewed as a stationary or propagating porosity wave respectively. Fluid pockets transported via a porosity wave remain relatively isolated with minimal mixing between neighboring pockets. We further show that the velocity of fluid flow can be significantly enhanced by the emergence of a porosity wave. The concept and the related model presented in this paper provide a unified consistent explanation for the key features observed in fluid flow in rock salt. The similar process is expected to occur in other deformable low-permeability media such as shale and partially molten rocks under a deviatoric stress. Thus, the result presented here has an important implication to hydrocarbon expulsion from shale source rocks, radioactive waste isolation in a tight rock repository, and caprock integrity of a subsurface gas (CO2, H2 or CH4) storage system. It may also help develop a new engineering approach to fluid injection into or extraction from unconventional reservoirs.

Review on the Study of Microscopic Pore Structure and Seepage Characteristics

Pore structure, as the focus of researchers, is also one of the main contents of reservoir geological research, and the characteristics of percolation depend on the micro-pore structure of the reservoir. The study of the characteristics of fluid percolation in the reservoir plays an important role in formulating a reasonable oil and gas exploitation scheme. Understanding the micro pore structure of the reservoir, analyzing the pore throat structure inside the oil and gas reservoir, and systematically studying the micro seepage characteristics of the reservoir have far-reaching influence and great significance for the exploration and development of oil and gas fields.

Heavy halogen compositions of peridotite massifs in the Ivrea-Verbano Zone and implications for strong modification of mantle rocks

The lithospheric mantle affected by metasomatism is an important reservoir for halogens and often preserves significant heterogeneity in halogen abundances. However, the halogen compositions of the non-metasomatized mantle rocks with depleted geochemical features have not been well studied, impeding a comprehensive understanding of the halogen distribution in the mantle. The Balmuccia (BM) and Baldissero (BD) peridotite massifs in the Ivrea-Verbano Zone, Italian Alps, represent fragments of the sub-continental lithospheric mantle (SCLM) that are fresh and devoid of subduction-related mantle metasomatism. Here we examine the heavy halogen contents of well-characterized spinel-facies mantle peridotites (n = 20) and pyroxenites (n = 16) from the BM and BD massifs. The results indicate significant variation in whole rock halogen contents of peridotites and pyroxenites, with chlorine (Cl) ranging from <30 to 244 μg/g, bromine (Br) from <0.10 to 0.94 μg/g, and iodine (I) from <0.015 to 0.12 μg/g. Some peridotites and pyroxenites show rather low halogen contents (below the detection limit), consistent with their depleted features. However, a large number of samples display halogen contents far higher than the depleted MORB mantle (i.e., Cl: 3–10 μg/g) and peridotite xenoliths from the strongly metasomatized mantle, although ∼30 % of the halogens were leached out by ultrapure water or dilute nitric acid. The data suggest variable halogen enrichment in many mantle rocks. The halogen contents of different types of mantle rocks do not vary with major elements and incompatible trace elements (e.g., Nb), indicating that halogen compositions are not controlled by magmatic processes such as melting and melt-peridotite reaction. Instead, secondary fluid inclusions are abundant and mainly distributed as trails within olivine and pyroxene in both peridotites and pyroxenites, which were likely trapped during the exhumation of these mantle rocks. Their elevated halogen/Nb ratios compared with MORB mantle and strong correlations with Ba/Nb support the effect of fluids. Micro-Raman analyses identify hydrous serpentines within fluid inclusions. Moreover, mass balance calculation indicates that halogen enrichment is mainly caused by secondary fluids (> 90 %). Together, our findings highlight that the fluid percolation in the mantle rocks during exhumation can intensely modify the halogens, to a similar degree as mantle metasomatism, suggesting the sensitivity of halogens to secondary modification.

Toward the apoplast metabolome: Establishing a leaf apoplast collection approach suitable for metabolomics analysis

The leaf apoplast contains several compounds that play important roles in the regulation of different physiological processes in plants. However, this compartment has been neglected in several experimental and modelling studies, which is mostly associated to the difficulty to collect apoplast washing fluid (AWF) in sufficient amount for metabolomics analysis and as free as possible from symplastic contamination. Here, we established an approach based in an infiltration-centrifugation technique that use little leaf material but allows sufficient AWF collection for gas chromatography mass spectrometry (GC-MS)-based metabolomics analysis in both tobacco and Arabidopsis. Up to 54 metabolites were annotated in leaf and apoplast samples from both species using either 20% (v/v) methanol (20% MeOH) or distilled deionized water (ddH2O) as infiltration fluids. The use of 20% MeOH increased the yield of the AWF collected but also the level of symplastic contamination, especially in Arabidopsis. We propose a correction factor and recommend the use of multiple markers such as MDH activity, protein content and conductivity measurements to verify the level of symplastic contamination in MeOH-based protocols. Neither the concentration of sugars nor the level of primary metabolites differed between apoplast samples extracted with ddH2O or 20% MeOH. This indicates that ddH2O can be preferentially used, given that it is a non-toxic and highly accessible infiltration fluid. The infiltration-centrifugation-based approach established here uses little leaf material and ddH2O as infiltration fluid, being suitable for GC-MS-based metabolomics analysis in tobacco and Arabidopsis, with great possibility to be extended for other plant species and tissues.

Coupling reservoir depletion and geomechanics to assess risks during post-blowout well capping: Case study on Macondo

Reservoir depletion can be consequential to wellbore integrity after a blowout, especially offshore. A prolonged post-blowout discharge extends reservoir depletion. “Underground blowouts” (tensile-fracture initiations) occurring after well capping, or shear-driven slow slippage of naturally-occurring pre-existing faults (PEFs) in the near-well vicinity, can compromise post-blowout wellbore integrity. Upward propagation of the initiated tensile fractures may trigger seafloor broaching by reservoir hydrocarbons.This study examines reservoir depletion analytically, evaluating associated geomechanical implications on the in-situ reservoir conditions and assessing the likelihood of tensile-fracture initiation (oriented longitudinally or transversely-to-the-wellbore) during post-blowout-well-capping operations, in addition to shear-driven slow slippage along PEFs in the near-well vicinity. A set of calculational procedures and thinking sequences are presented, necessary for encompassing the primary effects of post-blowout reservoir depletion on the in-situ stress state and the limits of tensile and shear failures that could aid in the appropriate blowout-contingency decision-making.Our novel, physics-based scheme (analytical-coupling approach) is applied to parameters from the MC 252–1 “Macondo Well” blowout from April 20, 2010 and the targeted M56 oil reservoir in deepwater Gulf of Mexico (GoM). The reservoir rock is modeled as a porous-permeable medium, considering fluid infiltration to-and-from the pressurized wellbore. The likelihood of an underground blowout correlates with the shut-in wellbore pressure buildup, after successful well capping.Elevated reservoir depletion via higher post-blowout-discharge flowrates and longer post-blowout-discharge periods (in terms of time duration) are shown to reduce the shut-in wellbore pressure buildup against time following well capping. The “critical discharge flowrate,” an established predictive indicator for underground blowouts following shut-in of an installed subsea-capping stack (SCS) is employed, using data from the post-blowout-discharge period, suggesting underground blowouts to be highly-unlikely for the set of parameters assessed. Finally, the Mohr-Coulomb criterion indicates that shear-driven slow slippage along PEFs in the near-well vicinity is also unlikely, considering the Macondo Well's bottomhole-wellbore-pressure history in the aftermath of the blowout.

Single-Cell RNA Sequencing Reveals Molecular Signatures that Distinguish Allergic from Irritant Contact Dermatitis

Allergic contact dermatitis (ACD) is a pruritic skin disease caused by environmental chemicals that induce cell-mediated skin inflammation within susceptible individuals. Irritant contact dermatitis (ICD) is caused by direct damage to the skin barrier by environmental insults. Diagnosis can be challenging as both types of contact dermatitis can appear similar by visual exam, and histopathological analysis does not reliably distinguish ACD from ICD. To discover specific biomarkers of ACD and ICD, we characterized the transcriptomic and proteomic changes that occur within the skin during each type of contact dermatitis. We induced ACD and ICD in healthy human volunteers and sampled skin using a non-scarring suction blister biopsy method that collects interstitial fluid and cellular infiltrate. Single cell RNA-sequencing analysis revealed that cell-specific transcriptome differences rather than cell type proportions best distinguished ACD from ICD. Allergy-specific genes were associated with upregulation of IFNG, and cell signaling network analysis implicated several other genes such as IL4, despite their low expression levels. We validated transcriptomic differences with proteomic assays on blister fluid and trained a logistic regression model on skin interstitial fluid proteins that could distinguish ACD from ICD and healthy control skin with 93% sensitivity and 93% specificity.

Footwall refrigeration versus footwall hydration: Reassessing steep thermal gradients below detachment faults with in situ SIMS oxygen isotope analysis

The extensional detachment systems of metamorphic core complexes (MCC) have the potential to facilitate significant fluid movement between Earth's surface and the warm, ductile middle crust. One long-standing and influential detachment fault-fluid interaction model called the “footwall refrigeration hypothesis” (Morrison and Anderson, 1998) postulates that cool meteoric fluids circulating downward along a detachment can facilitate large-scale heat loss at depth before rocks are exhumed by faulting. Stable isotopes of oxygen and hydrogen have long been recognized as potentially useful tracers of surface-to-depth fluid flow in core complexes; however, typical sampling methods and the competing effects between temperature and fluid composition have made oxygen isotope signatures of meteoric fluid infiltration difficult to detect and/or interpret in the rock record.Here, we use in situ δ18O measurements by secondary ion mass spectrometry (SIMS) to address the rock record of meteoric fluid interactions and temperature variation within the Whipple Mountains MCC footwall beneath the Whipple detachment fault (WDF). The micro-scale sampling of SIMS (10 µm diameter x 1–2 µm deep pits) allows investigation of isotope variability as a function of mineral geochemistry and microstructure and enables more accurate interpretation of the causes of isotope variability. In the Whipple footwall mylonites, quartz and epidote show grain-to-grain oxygen-isotope variability within samples and as a function of distance from the main detachment. Approaching the WDF, both quartz and epidote show increasing spread toward low δ18O values. The lowest SIMS-measured δ18O epidote values require exchange with a low δ18O, meteoric fluid at high temperature. However, SIMS data also reveal that meteoric fluid-rock interactions were spatially heterogeneous at the scale of individual grains, resulting in local preservation of metamorphic quartz-epidote oxygen isotope equilibrium unaffected by infiltrating meteoric fluid. Within preserved metamorphic domains at all investigated structural levels of the WDF footwall, quartz and epidote δ18O values are tightly clustered and yield similar fractionations (∆18Oqz-ep). For samples from the longest footwall traverse, these ∆18Oqz-ep values are 4.0 ± 0.4‰, consistent with δ18O equilibrium at 521+43/-37 °C. We conclude that there is no evidence of a large paleo-thermal gradient associated with the Whipple Detachment Fault (i.e., no evidence for footwall refrigeration).

Tunable electrical/thermal output performance of the PV/T system with magnetic nanofluid based spectral beam filter

The photovoltaic/thermal systems with magnetic nanofluid based spectral beam filter would achieve thermal decoupling and utilize the full spectrum to minimize energy loss. The prepared Fe3O4 magnetic nanofluid was introduced into this system as selective absorbing fluid filter. An external magnetic field was added to manipulate the distribution behavior of nanoparticles that affect the optical characteristics of magnetic nanofluids could meet the various application requirements for electrical/thermal output of the photovoltaic/thermal system. The optical properties of magnetic nanofluid with various nanoparticle distribution behaviors under the varying magnetic field were tested by UV–visible spectrophotometric and Finite-Difference Time-Domain simulated respectively. Furthermore, the effect of magnetic nanoparticle concentration and magnetic field strength on the electrical/thermal output performance of the photovoltaic/thermal system was studied. The results show the higher nanoparticle concentration enhances the thermal output of the system but reduces the electrical output, accompanied by a significant reduction in the surface temperature of the cell. Both photothermal and photovoltaic conversion efficiencies are increased below 100mT magnetic field due to the transmission in the response spectrum of monocrystalline silicon cell increase under the formation of chain structure of the nanoparticles. It is concluded that the photothermal, photovoltaic, and total solar energy conversion efficiencies are respectively 59.82%, 13.98%, and 73.75% at 0.0025 wt% concentration under 100 mT magnetic fields, and its function of merit value was increased by 109% compared to the system without spectral beam filter.

Multiscale discrete fracture network modelling of shallow-water carbonates: East Agri Valley Basin, Southern Italy

We investigate the multiscale geometrical and dimensional properties of fracture and fault networks crosscutting Lower Jurassic to Cretaceous shallow-water carbonate rocks. The carbonates are exposed along the flanks of the Viggiano Mt., on the eastern side of the High Agri Valley Basin of southern Italy. Furthermore, we employ the results of field and digital structural analyses to derive the input parameters for subsequent Discrete Fracture Network modelling (DFN) of geocellular volumes whose dimension and architecture resemble those of the investigated sites. DFN modelling is carried out for geocellular volumes representing the studied bed packages (5m-side volumes), outcrops (50m-side volumes), and reservoir-scale carbonate cliffs (500m-side volumes). Notably, modelling is obtained considering the minimum mechanical aperture value obtained in the field for porosity computations and theoretical values of fracture hydraulic aperture for permeability computations. This is because the mechanical aperture values and roughness profiles collected in the field along single fractures are unreliable due to weathering and localized karst-related dissolution. Our findings reveal that a scale-dependent geometry characterizes the Cretaceous carbonates over three orders of magnitude. These results support previously published data, and contrast with those obtained for the Lower Jurassic carbonates, which suffer of some bias due to the quality of the exposures. After DFN modelling, we show that the amount of computed fracture porosity is mainly due to the SB and NSB fractures, and that equivalent permeability is greater within faulted rock volumes. In terms of horizontal permeability, which is the most reliable result after DFN modelling, we document near isotropic horizontal permeability ellipses at all scales of observations. At individual outcrops, we note that the permeability ellipses are elongated parallel to the dominant fault sets that denotes how localized strain affects the directionality of fluid flow. Finally, we summarize the original data in a conceptual model illustrating the modalities of meteoric fluid infiltration through the vadose zone, and then subsequent horizontal flow in the phreatic zone present within the fracture carbonates at shallow depths.

Improving Shale Stability through the Utilization of Graphene Nanopowder and Modified Polymer-Based Silica Nanocomposite in Water-Based Drilling Fluids

Shale formations present significant challenges to traditional drilling fluids due to fluid infiltration, cuttings dispersion, and shale swelling, which can destabilize the wellbore. While oil-based drilling fluids (OBM) effectively address these concerns about their environmental impact, their cost limits their widespread use. Recently, nanomaterials (NPs) have emerged as a promising approach in drilling fluid technology, offering an innovative solution to improve the efficiency of water-based drilling fluids (WBDFs) in shale operations. This study evaluates the potential of utilizing modified silica nanocomposite and graphene nanopowder to formulate a nanoparticle-enhanced water-based drilling fluid (NP-WBDF). The main objective is to investigate the impact of these nanoparticle additives on the flow characteristics, filtration efficiency, and inhibition properties of the NP-WBDF. In this research, a silica nanocomposite was successfully synthesized using emulsion polymerization and analyzed using FTIR, PSD, and TEM techniques. Results showed that the silica nanocomposite exhibited a unimodal particle size distribution ranging from 38 nm to 164 nm, with an average particle size of approximately 72 nm. Shale samples before and after interaction with the graphene nanopowder WBDF and the silica nanocomposite WBDF were analyzed using scanning electron microscopy (SEM). The NP-WBM underwent evaluation through API filtration tests (LTLP), high-temperature/high-pressure (HTHP) filtration tests, and rheological measurements conducted with a conventional viscometer. Experimental results showed that the silica nanocomposite and the graphene nanopowder effectively bridged and sealed shale pore throats, demonstrating superior inhibition performance compared to conventional WBDF. Post adsorption, the shale surface exhibited increased hydrophobicity, contributing to enhanced stability. Overall, the silica nanocomposite and the graphene nanopowder positively impacted rheological performance and provided favorable filtration control in water-based drilling fluids.