Number Of Filaments Research Articles

Physical mechanism reveals bacterial slowdown above a critical number of flagella.

Numerous studies have explored the link between bacterial swimming and the number of flagella, a distinguishing feature of motile multi-flagellated bacteria. We revisit this open question using augmented slender-body theory simulations, in which we resolve the full hydrodynamic interactions within a bundle of helical filaments rotating and translating in synchrony. Unlike previous studies, our model considers the full torque-speed relationship of the bacterial flagellar motor, revealing its significant impact on multi-flagellated swimming. Because the viscous load per motor decreases with the flagellar number, the bacterial flagellar motor transitions from the high-load to the low-load regime at a critical number of filaments, leading to bacterial slowdown as further flagella are added to the bundle. We explain the physical mechanism behind the observed slowdown as an interplay between the load-dependent generation of torque by the motor, and the load-reducing cooperativity between flagella, which consists of both hydrodynamic and non-hydrodynamic components. The theoretically predicted critical number of flagella is remarkably close to the values reported for the model organism Escherichia coli. Our model further predicts that the critical number of flagella increases with viscosity, suggesting that bacteria can enhance their swimming capacity by growing more flagella in more viscous environments, consistent with empirical observations.

Experimental study on the effect of discharge parameters of high-frequency nSDBD on the multi-point ignition characteristics of NH3/air mixture

Multi-point ignition by high-frequency nanosecond surface dielectric barrier discharge (nSDBD) offers the potential to enhance the combustion speed of NH3/air mixtures. This paper investigates the ignition process of high-frequency nSDBD in NH3/air mixture. Firstly, the morphology of the discharge channels is studied, and it is found that before the formation of a clear flame kernel, with the increase of the pulse number, the discharge filament length increases first and then stabilized, while the discharge filament number decreases from about 20 to around 12. Secondly, the influence of the mixed gas state on discharge characteristics is studied, and it is observed that as the gas pressure increases, the single pulse energy decreases and the discharge filament length decreases, but the deposition energy per unit length did not change significantly. The increase in NH3 concentration leads to a slight decrease in discharge energy and length. Finally, the effect of the flame kernel on discharge morphology is studied, and it is found that when the flame kernel is small, the discharge filament trees change from dispersed to coincident, and the discharge energy is concentrated. When the flame kernel is large, the discharge filament trees mostly transform into overlapping types, and the number decreases.

Sample Thickness and Edge Proximity Influence Spatial Behavior of Filaments and Treatment Uniformity of RF Cold Atmospheric Pressure Plasma Jet

AbstractThe ability of atmospheric pressure plasma jets to treat complex non-planar surfaces is often cited as their advantage over other atmospheric plasmas. However, the effect of complex surfaces on plasma parameters and treatment efficiency has seldom been studied. Herein, we investigate the interaction of the atmospheric pressure plasma slit jet (PSJ) with block polypropylene samples of different thicknesses (5 and 30 mm) moving at two different speeds. Even though the distance between the slit outlet and the sample surface was kept constant, the treatment efficiency of PSJ ignited in the Ar and $$\hbox {Ar/O}_2$$ Ar/O 2 gas feeds varied with the sample thickness due to the plasma parameters such as filament count and speed being affected by the different distances of the ground (the closer the ground is, the higher the discharge electric field). On the other hand, the $$\hbox {Ar/N}_2$$ Ar/N 2 PSJ diffuse plasma plumes were less affected by the changes in the electric field, and the treatment efficiency was the same for both sample thicknesses. Additionally, we observed a difference in the efficiency and uniformity of the PSJ treatment of the edges and the central areas in some working conditions. The treatment efficiency near the edges depended on the duration of the filament contact, i. e., how long the local electric field trapped the filaments. Conversely, the treatment uniformity near the edges and in the central areas was different if the number of filaments changed rapidly as the discharge moved on and off the sample (the 5 mm samples treated by easily sustained Ar PSJ).

Research on high-velocity ballistic impact of para-aramid sateen fabric with ZnO nanorods oriented to the development of soft body armor

This study introduces an innovative approach of utilizing ZnO nanorod growing technology to the sateen fabric in order to improve its ballistic performance. Through SEM, FTIR, XRD and XPS analyses, ZnO nanorods with majority length of 1000–1500 nm and fineness of 200–600 nm have been deposited on the surface of sateen fabric surface Compared the fabric with ZnO nanorods (S-11-Nrs) with its neat counterpart, the mechanical properties of S-11-Nrs are increased, especially the inter-filament friction and fabric tensile breaking strength. However, in the impact process, the number of filaments involved in the impact is reduced and the filaments are mainly failure by the shear action of the bullets. Due to these positive and negative effects, the energy absorption of S-11-Nrs displays inferior ballistic energy absorption. Nevertheless, at lower areal density, in the non-perforation test, S-11-Nrs presents less BFS and shows better resistance to the consecutive shotting process compared with the neat fabric. The reason is that the increase of inter-filament friction limits the movement of filaments and thus results in less BFS. In addition, the flexibility, air permeability and moisture permeability are still kept. This research offers valuable insights for the design and development of comfortable soft body armor.

Experimental study on the ballistic performance of CFRP/AFB sandwich plate

Lightweight bulletproof plate is highly demanded in the field of security field. A novel plate of carbon fiber reinforced plate (CFRP)/aramid filament bundles (AFB) sandwich plate is manufactured through winding methods in this paper. The ballistic performance is evaluated and failure mechanism is explored on the designed six CFRP/AFB sandwich plates. It is found that the sandwich structure of 3-4 × 16 is the best structure by comprehensively considering the energy absorption, Coefficient of Variation(CV) and shaping difficulty, of which the v50 and the average energy absorption are 225.26 m/s and 150.60 J, respectively. In addition, for the case that the number of filaments in the first entanglement is more and the number of first entanglement in the secondary entanglement is less, the ballistic performance would be better. To the failure mechanism, the upper CFRP is damaged by shear failure and the bottom CFRP shows delamination and separation in the impact process. The filament bundle plate in the core layer is benefit in resistance to projectile impact and it fails mainly by the disintegration and bending. This novel CFRP/AFB sandwich plate is a new direction for producing ballistic proof plate.

The Effects of Polyester Filament Number and Accompanying Hydrophilic Fiber Type on Moisture Management and Drying of Double-Layered Knitted Fabrics

This study aims to investigate the moisture transfer and drying behavior of the double-layered fabrics knitted using polyester yarns with a combination of cotton and viscose yarns. As well as the fiber type on both faces, the effect of the number of filaments of the polyester yarns was also researched. For this purpose, double-layered interlock fabrics were knitted, and the resultant samples were characterized by moisture management, drying, water vapor permeability, and heat conduction tests. It was indicated that the fabrics knitted by polyester yarns on the inner side and cotton fabrics on the outer side resulted in a very good overall moisture-management capacity. The same applied to the drying speed; even faster drying was achieved than the 100% polyester double-layered samples. The increase in the filament number led to a decrease in both moisture management and drying rate. Moreover, it was determined that the use of viscose yarn was not suitable.

DATA DRIVEN AND CELL SPECIFIC DETERMINATION OF NUCLEI-ASSOCIATED ACTIN STRUCTURE

In the specialized field of cellular mechanosignaling, the intricate relationship between the cytoskeleton and nucleus is crucial for determining the behavior and fate of mesenchymal stem cells (MSCs). Existing analytical methods for filamentous actin fibers (F-actin) suffer from issues of accuracy and reproducibility. To address this, our research introduces a significant advancement in image analysis by utilizing two deep-learning segmentation Convolutional Neural Networks (CNNs) based on U-Net architecture. This algorithm precisely quantifies F-actin and offers reproducible data from 3D confocal microscopy images. Our CNNs automatically extract the 3D shape of both the nucleus and individual actin fibers, thereby enabling the geometric reconstruction of these critical cellular components. We applied this methodology to MSCs exposed to low-intensity vibration, and hypothesized that mechanical stimuli will induce perinuclear actin remodeling. Confocal Z-stack images of MSCs were captured using a 40x oil lens and a Zeiss LSM 900 microscope. Nucleus and F-actin structures were stained with Hoechst 33342 and Phalloidin, respectively. Nucleus-based images were employed to mask and isolate actin fibers within the nuclear vicinity. A specialized deep-learning segmentation model was engineered within the PyTorch framework. For model training, a selective 2% of nuclei and F-actin images were annotated. Specifically, three layers from each of six representative nuclei, among approximately 500 cross-sectional layers, were meticulously selected; a similar approach was employed for actin. The model underwent 200 epochs of training. Post-training, the deep-learning model was employed to process all collected cross-sectional images of F-actin and nuclei. A subsequent analytical step involved the scrutiny of intersecting segmented fibers between consecutive layers for geometrically accurate actin fiber reconstruction. We analyzed 102 control and 110 vibration-treated mesenchymal stem cells (MSCs) by utilizing our deep-learning-based reconstruction algorithm on confocal Z-stack images. Our data revealed an 8% decrease in nuclear height from 5.73 µm to 5.28 µm (p < 0.001) upon application of low-intensity vibration. In contrast, the two groups observed no significant difference in nuclear volume. Accompanying these changes at the nuclear level, the following changes in the F-actin cytoskeleton were observed upon LIV application. The total number of actin filaments per cell increased from 117 to 150. The average volume of individual actin fibers rose from 0.40 µm³ to 0.43 µm³. Notably, the volume of fibers at the apical nuclear surface increased from 0.35 µm³ to 0.46 µm³. While the average length of actin fibers in the apical nuclear surface remained largely unchanged due to the addition of smaller fibers in LIV groups, the LIV group had a larger number of fibers that were larger than 15µm.

Collective effect of Vigna sp. (mung) tubulin GTP hydrolysis rate divergence on microtubule filament assembly.

Microtubules (MTs) are dynamic cytoskeletal filaments with highly conserved sequences across evolution, polymerizing by the GTP-dependent assembly of tubulin subunits. Despite the sequence conservation, MT polymerization kinetics diverge quantitatively between vertebrate brain, the model plant Arabidopsis and the protozoan Plasmodium. Previously, tubulin purified from seedlings of the plant Vigna sp. (mung) by temperature cycling was found to have a very low critical concentration. However, the lengths of MTs were sub-micron, much shorter than brain tubulin filaments. This was explained in simulations to be the result of the collective effect of high nucleation and GTP hydrolysis rates. Here, we test the effect of GTPase rates of affinity-purified Vigna sp. tubulin on microtubule polymerization and elongation. Affinity-purified mung tubulin is active and has a critical concentration of .37 μM. The GTP-dependent polymerization kinetics are transient, consistent with previous results. Polymerization is stabilized in the presence of either GTP analog GMPPNP (non-hydrolyzable) or GMPCPP (slow-hydrolyzable). Using interference reflection microscopy (IRM) we find polymerization with the non-hydrolysable analog significantly increases filament numbers, while lengths are unaffected for both GTP analogs. However, prolonged incubation with slow-hydrolyzable GMPCPP results in long filaments, pointing to GTP hydrolysis as a key factor determining MT length. We find the average GTPase turnover number of mung tubulin is 22.8 min-1, compared to 2.04 min-1 for goat brain tubulin. Thus modulating GTPase rates affects both nucleation and elongation. This quantitative divergence in kinetics despite high sequence conservation in the GTPase domains of α- and β-tubulin could help better understand the roles of selective pressure and function in the diverse organisms.

Investigation on the Regulation of the Combustion Characteristics of a Propane/Air Mixture by Repetitive nSDBD Pretreatment.

This paper investigates the effect of the transient repetitive nanosecond surface dielectric barrier discharge (nSDBD) pretreatment on the combustion characteristics of combustible mixtures. A method of using nSDBD online pretreatment of a combustible mixture to regulate the combustion characteristics of the mixture is proposed. The study is conducted in a constant-volume combustion chamber at atmospheric pressure and temperature using a propane/air mixture as fuel. The discharge characteristics of repetitive nSDBD and the effects of pretreatment discharge energy and interval time between the pretreatment and ignition on combustion rate are discussed. The results show that (1) For 12.5 kHz pulses, the filament length generated with the annular dielectric barrier electrode discharge can reach up to 7 mm, and the number of discharge filaments per pulse can reach 40. (2) Pretreating mixtures using repetitive nSDBD at millisecond time scale can significantly improve the combustion process. (3) Repetitive nSDBD pretreatment can enhance the activity of the mixture near the surface of nSDBD electrode, which leads to obvious wrinkles on the flame surface and significantly improves the combustion rate. (4) The flame rise time decreases with the increase of discharge energy and increases with the increase of the interval time between the pretreatment and ignition. The effect of the repetitive nSDBD pretreatment on flame propagation during the flame development period becomes more pronounced with the increase of the excess air coefficient. The research results of the paper reveal the law of online regulation of combustion characteristics of combustible mixtures by the nSDBD, providing experimental data support for the mechanism research of online regulation of combustion characteristics of combustible mixtures by the nSDBD.

Cardiac Myosin and Thin Filament as Targets for Lead and Cadmium Divalent Cations.

Lead and cadmium are heavy metals widely distributed in the environment and contribute significantly to cardiovascular morbidity and mortality. Using Leadmium Green dye, we have shown that lead and cadmium enter cardiomyocytes, distributing throughout the cell. Using an invitro motility assay, we have shown that sliding velocity of actin and native thin filaments over myosin decreases with increasing concentrations of Pb2+ and Cd2+. Significantly lower concentrations of Pb2+ and Cd2+ (0.6mM) were required to stop sliding of thin filaments over myosin compared to the stopping actin sliding over the same myosin (1.1-1.6mM). Lower concentration of Cd2+ (1.1mM) needed to stop actin sliding over myosin compared to the Pb2++Cd2+ combination (1.3mM) and lead alone (1.6mM). There were no differences found in the effects of lead and cadmium cations on relative force developed by myosin heads or number of actin filaments bound to myosin. Sliding velocity of actin over myosin in the left atrium, right and left ventricles changed equally when exposed to the same dose of the same metal. Thus, we have demonstrated for the first time that Pb2+ and Cd2+ can directly affect myosin and thin filament function, with Cd2+ exerting a more toxic influence on myosin function compared to Pb2+.

Gill morphology adapted to oxygen-limited caves in Astyanax mexicanus.

Sensing and acquiring dissolved oxygen is crucial for nearly all aquatic life. This may become even more vital as dissolved oxygen concentrations continue to decline in many aquatic environments. While certain phenotypes that enable fish to live in low oxygen have been characterized, adaptations that arise following sudden, drastic reductions in dissolved oxygen are relatively unknown. Here, we assessed the blind Mexican cavefish, Astyanax mexicanus, for alterations to gill morphology that may be adaptive for life in hypoxic caves. The Astyanax system provides the unique opportunity to compare gill morphology between stereotypical "surface" adapted morphotypes and obligate cave-dwelling conspecifics. While the surface environment is well-oxygenated, cavefish must cope with significantly reduced oxygen. We began by quantifying traditional morphological gill traits including filament number and length as well as lamellar density and height in surface fish and two distinct cave populations, Pachón and Tinaja. This enabled us to estimate total lamellar height, a proxy for gill surface area. We then used immunohistochemical staining to label 5-HT-positive neuroepithelial cells (NECs), which serve as key oxygen sensors in fish. We discovered an increase in gill surface area for both cavefish populations compared to surface, which may enable a higher capacity of oxygen acquisition. Additionally, we found more NECs in Pachón cavefish compared to both surface fish and Tinaja cavefish, suggesting certain selective pressures may be cave-specific. Collectively, this work provides evidence that cavefish have adapted to low oxygen conditions via alterations to gill morphology and oxygen sensing, and informs evolutionary mechanisms of rapid adaptation to dramatic, chronic hypoxia.

Precipitation-induced filament pattern of injected fluid controlled by a structured cell.

Mixing of two fluids can lead to the formation of a precipitate. If one of the fluids is injected into a confined space filled with the other, then a created precipitate disrupts the flow locally and forms complex spatiotemporal patterns. The relevance of controlling these patterns has been highlighted in the engineering and geological contexts. Here, we show that such injection patterns can be controlled consistently by injection rate and obstacles. Our experimental results revealed filament patterns for high-injection and low-reaction rates, and the injection rate can control the number of active filaments. Furthermore, appropriately spaced obstacles in the cells can straighten the motion of the advancing tip of the filament. A mathematical model based on a moving boundary adopting the effect of precipitation reproduced the phase diagram and the straight motion of filaments in structured cells. Our study clarifies the impact of the nonlinear permeability response on the precipitate density and that of the obstacles in the surrounding medium on the motion of the injected fluid with precipitation.

Towards vortex-based wind turbine design using GPUs and wake accommodation

Vortex methods are often cited as a promising alternative to the classical Blade Element Momentum (BEM) theory for wind turbine design, overcoming some of its limitations such as imposing steady-state, uniform, and aligned inflows. BEM assumptions are being questioned, especially for large rotors with very flexible blades or even floating wind turbines. However, vortex methods are not yet widespread due to their computational costs. The following work demonstrates how free vortex wake methods using filament discretizations can become an affordable and viable alternative to BEM. This is achieved by combining filament number reduction as well as graphical processing units (GPUs) computations. The efficiency and accuracy of the proposed approaches are demonstrated against simple test cases, including an elliptical wing and a rigid horizontal axis wind turbine (HAWT). Finally, the approaches are applied to the aero-hydro-servo-elastic simulation of floating horizontal axis wind turbines.

Multi-scale collaborative prediction of optimal configuration for carbon fiber woven composites based on deep learning neural networks

Since carbon fiber woven composite panels contain complex fiber bundle structures, the aim of this paper is to investigate the factors influencing the macro-equivalent properties and topological flexibility of composite panels resulting from variations in fiber bundle configuration. Firstly, the structural topology optimization design of carbon fiber composite plates was achieved using a multi-scale modeling approach with the variable density method. Nonlinear relationships between carbon fiber bundle configuration parameters and macroscopic equivalent properties, as well as macroscale topological flexibility, were determined using deep learning methods, respectively. The maximum error in the predicted values of the deep learning network model does not exceed 0.5% when compared with the results of the finite element calculations. In addition, utilizing deep learning networks to predict macro-equivalent properties of composites can significantly decrease computational time. The impact of varying the number of filaments in fiber bundles and the spacing of the fiber bundles on the flexibility of the topology was determined using a deep learning network. The optimal topological flexibility, along with its corresponding fiber bundle spacing and number of fiber bundle filaments, are globally searched within the design domain using an adaptive genetic algorithm. The study in this paper can provide a reference for designing fiber bundle configurations of composites for practical applications.

Relatedness of hypoxia and hyperthermia tolerances in the Nile tilapia (Oreochromis niloticus) and their relationships with cardiac and gill traits

In fish, thermal and hypoxia tolerances may be functionally related, as suggested by the oxygen- and capacity-limited thermal tolerance (OCLTT) concept, which explains performance failure at high temperatures due to limitations in oxygen delivery. In this study the interrelatedness of hyperthermia and hypoxia tolerances in the Nile tilapia (Oreochromis niloticus), and their links to cardiorespiratory traits were examined. Different groups of O. niloticus (n = 51) were subjected to hypoxia and hyperthermia challenges and the O2 tension for aquatic surface respiration (ASR pO2) and critical thermal maximum (CTmax) were assessed as measurement endpoints. Gill filament length, total filament number, ventricle mass, length and width were also measured. Tolerance to hypoxia, as evidenced by ASR pO2 thresholds of the individual fish, was highly variable and varied between 0.26 and 3.39 kPa. ASR events increased more profoundly as O2 tensions decreased below 2 kPa. The CTmax values recorded for the O. niloticus individuals ranged from 43.1 to 44.8 °C (Mean: 44.2 ± 0.4 °C). Remarkably, there was a highly significant correlation between ASR pO2 and CTmax in O. niloticus (r = −0.76, p < 0.0001) with ASR pO2 increasing linearly with decreasing CTmax. There were, however, no discernible relationships between the measured cardiorespiratory properties and hypoxia or hyperthermia tolerances. The strong relationship between hypoxia and hyperthermia tolerances in this study may be related to the ability of the cardiorespiratory system to provide oxygen to respiring tissues under thermal stress, and thus provides some support for the OCLTT concept in this species, at least at the level of the entire organism.

A pull-out equivalent telescopic layered failure model of carbon fiber bundles in seawater sea-sand cementitious matrix

Based on the excellent mechanical properties and piezoresistive effect of carbon fiber, carbon fiber reinforced cementitious matrix (CFRCM) has been used in the strengthening and self-monitoring research of reinforced concrete (RC) structures. In this study, the assumptions of the telescopic layered model theory of CFRCM were refined. The factors influencing the piezoresistive effect of carbon fibers were subsequently analyzed. Simultaneously, the resistance calculation theory for the pull-out process of CFRCM bundles was established by combining the parallel circuit theory. Based on the above theories, a pull-out equivalent telescopic layered failure model of carbon fiber bundles in the cementitious matrix was developed, considering the trilinear cohesive material law (CML) and the piezoresistive effect. Pull-out tests on carbon fiber bundles in both freshwater standard sand and seawater sea-sand matrices were conducted. This model was successfully used to simulate the piezoresistive effect during the pull-out process of CFRCM bundles with two matrices and different embedded lengths. The number of fiber filaments in each layer, the rupture damage of the fiber filaments during the loading process, and the degree of matrix infiltration into the carbon fibers were calculated. This work lays the foundation for advancing the development of structural strengthening and structural health monitoring.

Multi-criteria optimization of a combined power and freshwater system using modified NSGA-II and AHP-entropy-topsis

This paper proposed an innovative hybrid system to generate electricity and fresh water with higher energy efficiency. A subsystem is employed by integrating DCMD and heat pump to recover waste heat of PEMFC to produce fresh water. The thermodynamic and exergoeconomic models are established to evaluate the system comprehensive performance. Compared with the PEMFC alone, the results demonstrated that the proposed hybrid system achieves a 42.07 % increase in energy efficiency and provides 177.86 kg/h of fresh water. The components with high exergy destruction are PEMFC, Relief value, Inverter, Separator 2 and DCMD with the exergy destruction rate of PEMFC stack accounting for 54.55 %. The parametric analysis results indicate that the variation of the membrane filament number, feed and permeate flow rates all have evident impacts on the thermodynamic and economic performances of the hybrid system. The multi-objective optimization is conducted by adopting NSGA-II and three decision-making methods. The optimal solution obtained by AHP-entropy-TOPSIS approach for the net power out, exergy efficiency, WPC, and total exergy cost rate are 96059W, 0.5293, 0.2995 $/m3, and 14.27 $/h, respectively. In addition, the obtained results shown that the simulation times of NSGA-II are significantly reduced from 89.30 to 11.20 h by introducing BP-ANN to improve the simulation efficiency.

Evolution of cosmic filaments in the MTNG simulation

We present a study of the evolution of cosmic filaments across redshift with an emphasis on some important properties: filament lengths, growth rates, and radial profiles of galaxy densities. Following an observation-driven approach, we built cosmic filament catalogues at z = 0, 1, 2, 3, and 4 from the galaxy distributions of the large hydro-dynamical run of the MilleniumTNG project. We employed the extensively used DisPerSE cosmic web finder code, for which we provide a user-friendly guide, including the details of a physics-driven calibration procedure, with the hope of helping future users. We performed the first statistical measurements of the evolution of connectivity in a large-scale simulation, finding that the connectivity of cosmic nodes (defined as the number of filaments attached) globally decreases from early to late times. The study of cosmic filaments in proper coordinates reveals that filaments grow in length and radial extent, as expected from large-scale structures in an expanding Universe. But the most interesting results arise once the Hubble flow is factored out. We find remarkably stable comoving filament length functions and over-density profiles, showing only little evolution of the total population of filaments in the past ∼12.25 Gyr. However, by tracking the spatial evolution of individual structures, we demonstrate that filaments of different lengths actually follow different evolutionary paths. While short filaments preferentially contract, long filaments expand along their longitudinal direction with growth rates that are the highest in the early, matter-dominated Universe. Filament diversity at a fixed redshift is also shown by the different (∼5σ) density values between the shortest and longest filaments. Our results hint that cosmic filaments can be used as additional probes for dark energy, but further theoretical work is still needed.

Decaying turbulence in molecular clouds: how does it affect filament networks and star formation?

ABSTRACT The fragmentation of gas to form stars in molecular clouds is intrinsically linked to the turbulence within them. These internal motions are set at the birth of the cloud and may vary with galactic environment and as the cloud evolves. In this paper, we introduce a new suite of 15 high-resolution 3D molecular cloud simulations using the moving mesh code arepo to investigate the role of different decaying turbulent modes (mixed, compressive, and solenoidal) and virial ratios on the evolution of a $10^4\, \mathrm{M}_{\odot }$ molecular cloud. We find that diffuse regions maintain a strong relic of the initial turbulent mode, whereas the initial gravitational potential dominates dense regions. Solenoidal seeded models thus give rise to a diffuse cloud with filament-like morphology, and an excess of brown dwarf mass fragments. Compressive seeded models have an early onset of star-formation, centrally condensed morphologies and a higher accretion rate, along with overbound clouds. 3D filaments identified using disperse and analysed through a new python toolkit we develop and make publicly available with this work called fiesta, show no clear trend in lengths, masses and densities between initial turbulent modes. Overbound clouds, however, produce more filaments and thus have more mass in filaments. The hubs formed by converging filaments are found to favour star-formation, with surprisingly similar mass distributions independent of the number of filaments connecting the hub.

Computational tools for quantifying actin filament numbers, lengths, and bundling.

The actin cytoskeleton is a dynamic filamentous network that assembles into specialized structures to enable cells to perform essential processes. Direct visualization of fluorescently-labeled cytoskeletal proteins has provided numerous insights into the dynamic processes that govern the assembly of actin-based structures. However, accurate analysis of these experiments is often complicated by the interdependent and kinetic natures of the reactions involved. It is often challenging to disentangle these processes to accurately track their evolution over time. Here, we describe two programs written in the MATLAB programming language that facilitate counting, length measurements, and quantification of bundling of actin filaments visualized in fluorescence micrographs. To demonstrate the usefulness of our programs, we describe their application to the analysis of two representative reactions: (1) a solution of pre-assembled filaments under equilibrium conditions, and (2) a reaction in which actin filaments are crosslinked together over time. We anticipate that these programs can be applied to extract equilibrium and kinetic information from a broad range of actin-based reactions, and that their usefulness can be expanded further to investigate the assembly of other biopolymers.