Identification of a locking segment is crucial to assess the potential runout risk and the overall stability of a high-locality landslide. However, it is difficult to identify a locking segment through field surveys before a landslide failure due to their concealment. Taking the Tizicao landslide—a high-locality landslide in Southwest China—as a field example, this study aims to identify and analyze a locking segment of the landslide. Based on geomorphology and spatial–temporal deformation of the landslide, the characteristics for identifying the locking segment of the Tizicao landslide are analyzed, and the locations and area of the locking segment are determined based on the analytical results. Furthermore, the identification results are interpreted through fieldwork including the displacement monitoring of ground surface and deep parts, borehole drilling, and oblique photography using unmanned aerial vehicles. The results reveal that the locking segment of the Tizicao landslide lies at the southern slope toe and covers an area of approximately 4.69 × 104 m2, accounting for 15.2% of the total area of the landslide. Significantly different characteristics are observed in respect of surface displacement, deep displacement, surface cracks, and sliding zone soil between locking and non-locking segment.