Field-contaminated Soils Research Articles

Precipitation patterns strongly affect vertical migration and methylation of mercury in legacy contaminated sites

Legacy-contaminated sites act as significant sources of mercury (Hg) to their surrounding surface and underground environments. Intensified extreme precipitation is posing great threats to the environment and human health by changing the fate of pollutants, yet little is known about its effect on the vertical migration and methylation of Hg in contaminated sites. Here, we applied a range of simulated extreme precipitation patterns (frequency and intensity) to column leaching assays with soils collected near a contaminated site. We observed that precipitation with high frequency but low intensity resulted in more vertical migration of Hg through the soil profile than that with low frequency but high intensity. The majority (> 90%) of leached Hg was prone to migrate vertically within the top 10 cm of the soil profile. Furthermore, rainfall stimulated microbial Hg methylation, as demonstrated by enhanced production of methylmercury (MeHg) in both simulated and field-contaminated soils. We identified specific microbial taxa including Geobacteraceae, Desulfuromonadaceae, Syntrophaceae, Oscillospiraceae, and Methanomicrobiaceae as key predictors of MeHg production, which differed from those typically observed in overlying water of croplands. Particularly, the relative abundance of these dominant Hg methylators significantly increased during rainfall-induced leaching compared to that of the control, suggesting the crucial yet previously overlooked impacts of increased precipitation events on the process of microbial Hg methylation in industry-contaminated sites. Given the rising incidences of extreme precipitation events worldwide due to climate change, this study highlights the significance of assessing Hg mobility and microbial transformation in legacy contaminated sites.

The defensin protein NtCAL1 functions as a positive factor in plant cadmium accumulation and resistance in tobacco

Cadmium (Cd) poses a threat to food safety and human health. Plant defensins are small peptides that involved in Cd metabolism. however, the function of tobacco phyto-defensin protein NtCAL1 in Cd metabolism remains poorly understood. Here we identified the effects of NtCAL1 from tobacco on Cd metabolism. We provide evidence that NtCAL1 is located in the cell wall and varied expressed in organs of developmental stages. The overexpression of NtCAL1 has been demonstrated to enhance the content of AsA and antioxidant enzyme (CAT and APX) activities, as well as to positively regulate the expression of NtRAMP3, NtHMAα and NtHMAβ. The NtCAL1 was identified as a positive regulator of cadmium (Cd) translocation from the root to the shoot. Furthermore, the accumulation of Cd was inhibited by Cd foliar inhibitors. In conclusion, we propose that NtCAL1 acts synergistically with other proteins involved in Cd uptake and transport, thereby enhancing Cd tolerance through the upregulation of antioxidant substances and the activation of antioxidant enzymes.The NtCAL1 provides a significant potential tool for the development of low-Cd tobacco, while remediation of Cd contaminated field soils.

Laccase-mediated degradation of petroleum hydrocarbons in historically contaminated soil

Laccases (EC1.10.3.2) have attracted growing attention in bioremediation research due to their high reactivity and substrate versatility. In this study, three genes for potential novel laccases were identified in an enrichment culture from contaminated field soil and recombinantly expressed in E. coli. Two of them, designated as PlL and BaL, were biochemically characterized regarding their optimal pH and temperature, kinetic parameters, and substrate versatility. In addition, lacasse PlL from Parvibaculum lavamentivorans was tested on historically contaminated soil. Treatment with PlL led to a significantly higher reduction of total petroleum hydrocarbons (83% w/w) compared to the microbial control (74% w/w). Hereby, PlL was especially effective in degrading hydrocarbons > C17. Their residual concentration was by 43% w/w lower than in the microbial treatment. In comparison to the laccase from Myceliophthora thermophila (MtL), PlL treatment was not significantly different for the fraction > C17 but resulted in a 30% (w/w) lower residual concentration for hydrocarbons < C18. In general, PlL can promote the degradation of petroleum hydrocarbons. As a consequence, it can be applied to reduce remediation time by duly achieving remediation target concentrations needed for site closure.

Evaluation of arsenic pollution in field-contaminated soil at the soil's actual pH

The pollution and ecological risks posed by arsenic (As) entering the soil are the major environmental challenges faced by human beings. Soil phosphatase can serve as a useful indicator for assessing As contamination under specific soil pH conditions. However, the study of phosphatase kinetics in long-term field As-contaminated soil remains unclear, presenting a significant obstacle to the monitoring and evaluation of As pollution and toxicity. The purpose of this study was to determine phosphatase activity and explore enzyme kinetics in soils subjected to long-term field As contamination. Results revealed that the soil phosphatase activity varied among the tested soil samples, depending on the concentrations of As. The relationship between total As, As fractions and phosphatase activity was found to be significant through negative exponential function fitting. Kinetic parameters, including maximum reaction velocity (Vmax), Michaelis constant (Km) and catalytic efficiency (Vmax/Km), ranged from 3.14 × 10−2–53.88 × 10−2 mmol/(L·hr), 0.61–7.92 mmol/L, and 0.46 × 10−2–11.20 × 10−2 hr−1, respectively. Vmax and Vmax/Km of phosphatase decreased with increasing As pollution, while Km was less affected. Interestingly, Vmax/Km showed a significant negative correlation with all As fractions and total As. The ecological doses (ED10) for the complete inhibition and partial inhibition models ranged from 0.22–70.33 mg/kg and 0.001–55.27 mg/kg, respectively, indicating that Vmax/Km can be used as an index for assessing As pollution in field-contaminated soil. This study demonstrated that the phosphatase kinetics parameters in the soil's pH system were better indicators than the optimal pH for evaluating the field ecotoxicity of As.

Propylene glycol-mixed steam enhanced extraction for an efficient and sustainable remediation of PAHs-contaminated soil

An innovative thermal desorption method, propylene glycol (PG)-mixed steam enhanced extraction, is proposed for a highly efficient remediation of polycyclic aromatic hydrocarbons (PAHs)-contaminated soil. It is found that injecting PG-mixed steam into soil column could obtain > 99% removal efficiencies of PAHs either for the pyrene-spiked soil, or for the contaminated field soil with high-molecular-weight PAHs. PG is a safe and low-cost dihydric alcohol with a boiling point higher than water. When the PG-mixed steam penetrated the contaminated soil, the PG vapor preferentially condensed to form a hot liquid with concentrated PG (e.g., from 30 wt% PG in gas phase to 90 wt% PG in the liquid phase), which would significantly solubilize the PAHs and enhance their desorption from soils. The results also revealed that the effluents derived from the PG-mixed steam could be purified by removing the desorbed PAHs using a simple coagulation treatment, and the recovered PG solution could be reused. The plant assay using wheat seeds showed that the remediated soil had a good regreening potential. Our results demonstrate that PG-mixed steam injection is a promising thermal desorption method for an efficient and sustainable remediation of PAHs-contaminated soil.

Exogenous plant growth regulator and foliar fertilizers for phytoextraction of cadmium with Boehmeria nivea [L.] Gaudich from contaminated field soil

As a enrichment plant, ramie can be used for the phytoremediation of cadmium (Cd)-contaminated soil. However, it is worth exploring the role of plant growth regulators and foliar fertilizers in the process of plant growth and development and Cd adsorption. By measuring the agronomic traits, Cd content of aboveground and underground ramie, calculating the Cd transfer coefficient (TF) and Cd bioconcentration factors (BCF), and the correlation between various indicators. This study examined the effects of plant growth regulators and foliar fertilizers on ramie’s capacity for Cd accumulation and transportation. Plant growth regulators and foliar fertilizers increased the Cd content of the aboveground ramie, reduced the Cd content of the underground ramie, and increased the TF. Among them, GA-1 increased the Cd content of the aboveground ramie to 3 times more than that of the control and reduced the Cd content of the underground ramie by 54.76%. Salicylic acid (SA) increased the Cd content of the aboveground ramie to three times more than that of the control. The combination of GA and foliar fertilizer reduced the Cd content of the aboveground and underground ramie and the TF and BCF of the underground ramie. After the hormones were sprayed, the TF of ramie had a significant positive correlation with the Cd content of the aboveground ramie; the BCF of the aboveground ramie had a significant positive correlation with the Cd content and TF of the aboveground ramie. The results indicate that Brassinolide (BR), gibberellin (GA), ethephon (ETH), polyamines (PAs), and salicylic acid (SA) have different effects on the enrichment and transport of Cd in ramie. This study provided an effective method to improve the capacity for ramie to adsorb heavy metals during cultivation.

Distribution and biodegradation of nonextractable polycyclic aromatic hydrocarbons in particle-size aggregates of field-contaminated soils

Distribution and biodegradation of nonextractable polycyclic aromatic hydrocarbons in particle-size aggregates of field-contaminated soils

The spatial distribution, health risk, and cytotoxicity of metal(loid)s in contaminated field soils: The role of Cd in human gastric cells damage

The spatial distribution and pollution level of heavy metal(loid)s in soil (0–6 m) from a typical industrial region in Jiangmen City, Southeast China was investigated. Their bioaccessibility, health risk, and human gastric cytotoxicity in topsoil were also evaluated using an in vitro digestion/human cell model. The average concentrations of Cd (87.52 mg/kg), Co (106.9 mg/kg), and Ni (1007 mg/kg) exceeded the risk screening values. The distribution profiles of metal(loid)s showed a downward migration trend to reach a depth of 2 m. The highest contamination was found in topsoil (0–0.5 m), with the concentrations of As, Cd, Co, and Ni being 46.98, 348.28, 317.44, and 2395.60 mg/kg, respectively, while Cd showed the highest bioaccessibility in the gastric phase (72.80 %), followed by Co (21.08 %), Ni (18.27 %), and As (5.26 %) and unacceptable carcinogenic risk. Moreover, the gastric digesta of topsoil suppressed the cell viability and triggered cell apoptosis, evidenced by disruption of mitochondrial transmembrane potential and increase of Cytochrome c (Cyt c) and Caspases 3/9 mRNA expression. Bioaccessible Cd in topsoil was responsible for those adverse effects. Our data suggest the importance to reduce Cd in the soil to decrease its adverse impacts on the human stomach.

Assessing the toxicity of contaminated soils via direct contact using a gas production bioassay of thiosulfate utilizing denitrifying bacteria (TUDB)

A direct contact bioassay of thiosulfate utilizing denitrifying bacteria (TUDB) based on inhibition of gas production was deployed to assess the toxicity of naturally contaminated field soils and soils artificially contaminated with heavy metals. Test procedure producing optimal conditions responsible for maximum gas production was 0.5 mL test culture, 1 g soil sample, 80 RPM, and 48 h reaction time. Similarly, the concentrations which generated a 50% reduction in gas production by TUDB for the tested heavy metals were 3.01 mg/kg Cr6+; 15.30 mg/kg Ni2+;15.50 mg/kg Cu2+;16.60 mg/kg Ag+; 20.60 mg/kg As3+; 32.80 mg/kg Hg2+; 54.70 mg/kg Cd2+; and 74.0 mg/kg Pb2+. Because soil toxicity is usually influenced by various physicochemical characteristics, ten reference soils were used to determine the toxicity threshold for evaluating the toxicity of naturally contaminated field soils. All eight contaminated soils were toxic to the TUDB bioassay because their levels of inhibition ranged between 72% and 100% and exceeded the determined toxicity threshold of 10%. Compared to other direct contact assays, the newly developed assay TUDB proved to be very robust, producing highly sensitive data while the different soil physicochemical properties exerted minimal influence on the gas production activity of TUDB. Additionally, the simplicity of the developed methodology coupled with the elimination of pretreatment procedures such as elutriation, and ability to perform generate sensitive data in turbid and highly colored samples makes it, cost-effective, and easily adaptable for the assessment of heavy metal and field contaminated soils when compared with other conventional assays which require sophisticated instrumentation and prolonged testing procedures and times.

Targeting sorbed PAHs in historically contaminated soil - Can laccase mediator systems or Fenton's reagent remove inaccessible PAHs?

This laboratory study investigates the potential of two innovative laccase-mediator systems for removing PAHs from historically contaminated field soil and focuses on the treatment effect on the accessible and desorption resistant PAH fraction. Laccase degraded accessible PAHs when applied in combination with the mediator TEMPO (up to 24% within 48h). The mediator HBT did not induce degradation but mobilized desorption resistant PAHs from high affinity sorption sites via a competitive sorption mechanism. Enzymatic degradation of inaccessible PAHs was not observed with neither of the two enzyme-mediator systems. To verify a potential radical susceptibility of contaminants inaccessible to microorganisms, PAH contaminated biochar was treated with hydroxyl radicals generated by Fenton's reaction. These radical species reduced the desorption resistant fraction of phenanthrene (13±10%), fluoranthene (33±8%) and benzo(a)pyrene (69±5%). In conclusion, laccase-mediator systems can interact with accessible and inaccessible PAHs, whereas direct degradation of desorption resistant contaminants required highly active hydroxyl radicals. Further studies should develop enzyme-mediator systems establishing a sufficient oxidation potential to attack the desorption resistant contaminant fraction.

Distribution patterns, toxic equivalence, and environmental risks of polycyclic aromatic hydrocarbons in different density fractions of long-term field-contaminated soils

Distribution patterns, toxic equivalence, and environmental risks of polycyclic aromatic hydrocarbons in different density fractions of long-term field-contaminated soils

Environmental conditions and plant physiology modulate Cu phytotoxicity in field-contaminated soils

Foliar Cu concentration has been widely used as a biomarker of plant growth in phytotoxicity bioassays. This relation has helped find plant processes altered by Cu in dose-response experiments (a bivariate approach). However, when plants are grown in field conditions, their responses can vary in function of multiple variables, such as the environment, plant physiology, and other elements in plant (plant ionome). These sources of variability are commonly unreported, which could limit bioassays’ utility. Thus, the present study aimed to assess and integrate the mentioned sources of variability on Cu phytotoxicity. Lettuce was used as plant model. Lettuces were grown in growth chambers with contrasting light and air humidity conditions and on two different field-contaminated soils (sandy and loamy soils). Results showed that environmental conditions significantly affected foliar Cu and plant growth, but this effect differed in the two studied soils. Foliar Cu was not a good biomarker of plant growth. In contrast, integrating the potential phytotoxicity effect with the plant's nutritional status allowed a better understanding of plant growth. We remarked on using a structural equation modeling approach (SEM) to integrate plant physiology and plant ionome as moderators of plant growth. Results showed that plant growth was primarily related to plant nutritional status rather than Cu phytotoxicity. Also, the foliar Cu concentration would affect plant nutritional status due to photosynthesis-related plant processes and cation balance. Finally, this research invites to state and include sources of variability when assessing phytotoxicity. This way, it is possible to advance toward understanding complex linked processes occurring in field conditions.

Fly ash-based waste for ex-situ landfill stabilization of per- and polyfluoroalkyl substance (PFAS)-contaminated soil

In response to world-wide soil and groundwater contamination per- and polyfluoroalkyl substances (PFAS), stakeholders require immediate mitigation. Soil deposition in landfill is a common mitigation scheme, but PFAS losses occur via landfill leachate. These leaching losses can be reduced by strategically utilizing other deposited waste materials for ex-situ contaminant stabilization. This screening study tested activated carbon (AC) and eight types of wastes (compost, rubber granulate, bentonite clay, industrial sludge, incineration slag, incineration bottom ash (n=4), incineration fly ash-based air pollution control residue (FA-APC) (n=16)) in amending (adding 4%, 5%, 10% or 25% sorbent) field-contaminated (n=19) and PFAS-fortified (n=11) soils. A subset of FA-based residue types, all originating from grate-fire incineration (G-F-I) plants, achieved extraordinarily high removal of PFAS. The removal was up to 98% (25% addition) of the sum of six dominant PFAS for field-contaminated soil and >99% of the sum of 11 PFAS for fortified soil (10/25% addition) (>99.9% for PFOS). Calculated partitioning coefficient revealed significant trends between sorption strength and perfluorocarbon chain length (0.21-0.47 log units per CF2-moiety), indicating high importance of hydrophobic sorption (R2>0.98). However, with incremental G-F-I FA-APC addition this relationship disappeared, indicating an alternative sorption mechanism. The exceptional PFAS sorption by G-F-I FA-APC was not explained by G-F-I surface area, surface charge, soil mineral- and metal composition, or solution DOC, metal, or ion composition (H+, Ca2+, Mg2+, Al3+ and Ba2+). Although the mechanism remains unknown, this study showed that landfill sites can utilize G-F-I FA-APC for ex-situ stabilization at negative cost, thus preventing PFAS-containing leachate.

A direct contact bioassay using immobilized microalgal balls to evaluate the toxicity of contaminated field soils

The present study used a bioassay of immobilized microalgae (Chlorella vulgaris) via direct contact to assess the toxicity of eleven uncontaminated (reference) and five field contaminated soils with various physicochemical properties and contamination. Photosynthetic oxygen concentration in the headspace of the test kit by Chlorella vulgaris in the reference soils ranged between 12.93% and 14.80% and only 2.54%–7.14% in the contaminated soils, respectively. Inherent test variability (CVi) values ranged between 2.90% and 9.04%; variation due to soil natural properties (CVrs) ranged between 0.33% and 13.0%; and minimal detectable difference (MDD) values ranged from 4.69% to 11.6%. A computed toxicity threshold of 15% was established for microalgae soil toxicity tests based on calculations of the maximal tolerable inhibition (MTI). All contaminated soils were considered toxic to microalgae because their levels of inhibition ranged between 39.5% and 82.9%, exceeding the 15% toxicity threshold. It can be concluded that the elevated concentrations of heavy metals and organic contaminants in the contaminated soils induced the higher inhibitory levels. Overall, direct contact soil toxicity tests using immobilized microalgae provided coherent and repeatable data and can be utilized as a simple and suitable tool for the toxicity testing of contaminated field soils.

Leaching and Transformation of Perfluoroalkyl Acids and Polyfluoroalkyl Phosphate Diesters in Unsaturated Soil Column Studies.

Per- and polyfluoroalkyl substances (PFAS) are environmentally ubiquitous, anthropogenic substances with adverse effects on organisms, which shows the need to study their environmental fate and leaching behavior. In the present soil columns study, the leaching behavior and fate of nontransformable and transformable (precursors) were investigated. Ten nontransformable PFAS in two different soils, two precursors and two field soils, which were already contaminated with a mixture of PFAS, and two uncontaminated controls, were set up for a time span of 2 years. At the end of the study, the molecular balance could not be closed for nontransformable PFAS. This effect was positively correlated to the fluorinated carbon chain length. The precursors, which were both polyfluoroalkyl phosphate diesters (diPAP), had different transformation products and transformation rates, with a higher rate for 6:2 diPAP than 8:2 diPAP. After 2 years, amounts of diPAP were still found in the soil with no significant vertical movement, showing high adsorption to soils. Transformation products were estimated to be simultaneously formed. They were predominantly found in the percolation water; the amounts left in soil were negligible. Up to half of the initial precursor amounts could not be balanced and were considered missing amounts. The results of contaminated field soil experiments showed the challenge to estimate PFAS leaching without knowing all occurring precursors and complex transformation dynamics. For this purpose, it was shown that a broad examination of contaminated soil with different analytical methods can help with qualitative estimations of leaching risks. For a better quantitative estimation, analytical determination of more PFAS and a quantification of the missing amounts are needed. Environ Toxicol Chem 2022;41:2065-2077. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Leaching potential of per- and polyfluoroalkyl substances from source zones with historic contamination of aqueous film forming foam - a surfactant mixture problem

Per-and poly fluoroalkyl substances (PFAS) from aqueous film forming foam (AFFF) used at firefighting training facilities (FTF) contaminate waterbodies and pose a threat to human health and the environment worldwide . In this study, leaching of PFAS from historically AFFF contaminated field soil has been investigated under saturated conditions using up-flow columns for four soil samples with varying PFAS concentrations to quantify the long-term release potential of the source zone. PFOS was the most abundant PFAS present in the soils (84–98% of ΣPFAS12), varying from 358 to 1097 µg/kg and the total organic carbon (TOC) content varied from 0.73 to 2.0%. The experiment was run until a liquid to solid (L/S) ratio of 10 was reached (equivalent to 80 pore volumes or approximately 100 years of natural infiltration at the sampled field site). Results show a general trend of decreasing concentrations of short chain PFAS with increasing leaching volume. However, PFOS concentrations initially increased by 45–87% in three samples with relatively low TOC (0.7, 1.0 and 1.4%), before being reduced. In the 2.0% TOC soil PFOS concentrations continued to increase throughout the experiment. The residual PFOS content in the four soil samples showed no correlation with TOC content in the soil. Up to 50% of the initial PFOS content was retained in the soil with the lowest TOC content (0.7%), whereas all PFOS was removed in the soil with a TOC content of 1.4%. It is hypothesized that DOC present in the eluates originates from other non-fluorinated components present in AFFF that can facilitate PFAS leaching.

Combining a Standardized Batch Test with the Biotic Ligand Model to Predict Copper and Zinc Ecotoxicity in Soils.

Extraction of soil samples with dilute CaCl2 solution in a routinely performed batch test has potential to be used in site‐specific assessment of ecotoxicological risks at metal‐contaminated sites. Soil extracts could potentially give a measure of the concentration of bioavailable metals in the soil solution, thereby including effects of soil properties and contaminant “aging.” We explored the possibility of using a 0.001 M CaCl2 batch test combined with biotic ligand models (BLMs) for assessment of ecotoxicity in soils. Concentrations of Cu2+ and Zn2+ in soil extracts were linked to responses in ecotoxicity tests (microbial processes, plants, and invertebrates) previously performed on metal‐spiked soils. The batch test data for soils were obtained by spiking archived soil materials using the same protocol as in the original studies. Effective concentration values based on free metal concentrations in soil extracts were related to pH by linear regressions. Finally, field‐contaminated soils were used to validate model performance. Our results indicate a strong pH‐dependent toxicity of the free metal ions in the soil extracts, with R 2 values ranging from 0.54 to 0.93 (median 0.84), among tests and metals. Using pH‐adjusted Cu2+ and Zn2+ concentrations in soil extracts, the toxic responses in spiked soils and field‐contaminated soils were similar, indicating a potential for the calibrated models to assess toxic effects in field‐contaminated soils, accounting for differences in soil properties and effects of contaminant “aging.” Consequently, evaluation of a standardized 0.001 M CaCl2 batch test with a simplified BLM can provide the basis for an easy‐to‐use tool for site‐specific risk assessment of metal toxicity to soil organisms. Environ Toxicol Chem 2022;41:1540–1554. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Remediation of phenol contaminated soil using persulfate activated by ball-milled colloidal activated carbon

The degradation of phenolic compounds through persulfate (PS) activation is a valuable approach for soil/groundwater remediation. Several reports have been made related to PS activation and contaminant degradation using carbo-catalysts; however, there is no detailed study on soil remediation by colloidal activated carbon. This study demonstrates the phenol (PhOH) degradation efficiency in spiked and field-contaminated soils by a novel and low-cost ball-milled colloidal activated carbon (CACBM) catalyst. The CACBM/PS system exhibited outstanding degradation performance for PhOH in both spiked and field-contaminated soils. Optimum condition for degradation of 5.63 mmol PhOH kg soil−1 was achieved at 2.5 mg CACBM g soil−1, 5 mM PS, and a solid-liquid ratio of 1:5 at 25 °C in the wide pH range of 3–11. Radical scavenger experiments and electron spin resonance (ESR) spectroscopy revealed that both radical (•OH and SO4•–) and non-radical (1O2) species were involved in the CACBM/PS system. PhOH degradation in soil phase followed several degradation pathways, resulting in various intermediate byproducts such as acetic acid, maleic acid, p-benzoquinone, fumaric acid, and ferulic acid as analyzed by ultra-high-performance liquid chromatography with mass spectroscopy (UPLC−MS). The CACBM/PS system showed a promising potential in the remediation of organic-contaminated soil.

Toxicokinetics of metals in the soil invertebrate Enchytraeus crypticus exposed to field-contaminated soils from a mining area

Toxicokinetics may help assessing the risk of metal-contaminated soils by quantifying the development of internal metal concentrations in organisms over time. This study assessed the toxicokinetics in Enchytraeus crypticus of non-essential (Pb and Cd) and essential elements (Zn and Cu) in metal-contaminated field soils from a mining area, containing 3.49–24.3 mg Cd/kg dry soil, 433–1416 mg Pb/kg dry soil, 15.7–44.9 mg Cu/kg dry soil and 1718–6050 mg Zn/kg dry soil. Three different uptake-elimination patterns in E. crypticus were found. Both essential elements (Zn and Cu) showed fast increasing internal concentrations reaching equilibrium within 2 d in the uptake phase, without hardly any elimination after transfer to clean soil. The non-essential Cd showed a slow linear accumulation and excretion with body concentrations not reaching steady state within 21 d. Internal Pb concentrations, however, reached equilibrium within 7 d in the uptake phase. Longer exposure times in ecotoxicological tests, therefore, are required for elements like Cd. Porewater pH and dissolved organic carbon (DOC) levels were the dominant factors controlling Cd uptake from the test soils. The 21-d body Cd and Pb concentrations were best explained from 0.01 M CaCl2-extractable soil concentrations. Steady-state Cu and Zn body concentrations were independent of soil exposure concentrations. Bioaccumulation factors (BAF) were low for Pb (<0.1 kgsoil/kgworm), but high for Cd at 1.78–24.3 kgsoil/kgworm, suggesting a potential risk of Cd biomagnification in the terrestrial food chain of the mining area ecosystem.

A Direct Contact Bioassay Using Immobilized Microalgal Balls to Evaluate the Toxicity of Contaminated Field Soils

A Direct Contact Bioassay Using Immobilized Microalgal Balls to Evaluate the Toxicity of Contaminated Field Soils