Arrangement Of Fibers Research Articles

Effects of freeze-drying bovine pericardium using a combination of polyethylene glycol and trehalose

The freeze-drying is a technology that preserves biological samples in a dry state, which is beneficial for storage, transportation, and cost saving. In this study, the bovine pericardium was treated with a freeze-drying protectant composed of polyethylene glycol (PEG) and trehalose (Tre), and then freeze-dried. The results demonstrated that the mechanical properties of the pericardium treated with PEG + 10% w/v Tre were superior to those of the pericardium fixed with glutaraldehyde (GA). The wet state water content of the rehydrated pericardium, determined using the Karl Fischer method, was (74.81 ± 1.44)%, which was comparable to that of the GA-fixed pericardium. The dry state water content was significantly reduced to (8.64 ± 1.52)%, indicating effective dehydration during the freeze-drying process. Differential scanning calorimetry (DSC) testing revealed that the thermal shrinkage temperature of the pericardium was (84.96 ± 0.49) ℃, higher than that of the GA-fixed pericardium (83.14 ± 0.11) ℃, indicating greater thermal stability. Fourier transform infrared spectroscopy (FTIR) results showed no damage to the protein structure during freeze-drying. Hematoxylin and eosin (HE) staining demonstrated that the freeze-drying process reduced pore formation, prevented ice crystal growth, and resulted in a tighter arrangement of tissue fibers. The frozen-dried bovine pericardium was subjected to tests for cell viability and hemolysis rate. The results revealed a cell proliferation rate of (77.87 ± 0.49)%, corresponding to a toxicity grade of 1. Additionally, the hemolysis rate was (0.17 ± 0.02)%, which is below the standard of 5%. These findings indicated that the frozen-dried bovine pericardium exhibited satisfactory performance in terms of cytotoxicity and hemolysis, thus meeting the relevant standards. In summary, the performance of the bovine pericardium treated with PEG + 10% w/v Tre and subjected to freeze-drying could meet the required standards.

Comparative analysis of traction forces in normal and glaucomatous trabecular meshwork cells within a 3D, active fluid-structure interaction culture environment

This study presents a 3D in vitro cell culture model, meticulously 3D printed to replicate the conventional aqueous outflow pathway anatomical structure, facilitating the study of trabecular meshwork (TM) cellular responses under glaucomatous conditions. Glaucoma affects TM cell functionality, leading to extracellular matrix (ECM) stiffening, enhanced cell-ECM adhesion, and obstructed aqueous humor outflow. Our model, reconstructed from polyacrylamide gel with elastic moduli of 1.5 and 21.7 kPa, is based on serial block-face scanning electron microscopy images of the outflow pathway. It allows for quantifying 3D, depth-dependent, dynamic traction forces exerted by both normal and glaucomatous TM cells within an active fluid-structure interaction (FSI) environment. In our experimental design, we designed two scenarios: a control group with TM cells observed over 20 hours without flow (static setting), focusing on intrinsic cellular contractile forces, and a second scenario incorporating active FSI to evaluate its impact on traction forces (dynamic setting). Our observations revealed that active FSI results in higher traction forces (normal: 1.83-fold and glaucoma: 2.24-fold) and shear strains (normal: 1.81-fold and glaucoma: 2.41-fold), with stiffer substrates amplifying this effect. Glaucomatous cells consistently exhibited larger forces than normal cells. Increasing gel stiffness led to enhanced stress fiber formation in TM cells, particularly in glaucomatous cells. Exposure to active FSI dramatically altered actin organization in both normal and glaucomatous TM cells, particularly affecting cortical actin stress fiber arrangement. This model while preliminary offers a new method in understanding TM cell biomechanics and ECM stiffening in glaucoma, highlighting the importance of FSI in these processes. Statement of significanceThis pioneering project presents an advanced 3D in vitro model, meticulously replicating the human trabecular meshwork's anatomy for glaucoma research. It enables precise quantification of cellular forces in a dynamic fluid-structure interaction, a leap forward from existing 2D models. This advancement promises significant insights into trabecular meshwork cell biomechanics and the stiffening of the extracellular matrix in glaucoma, offering potential pathways for innovative treatments. This research is positioned at the forefront of ocular disease study, with implications that extend to broader biomedical applications.

Macro- or Microfiber Scaffolds with Different Fiber Arrangements: Determining the Best Design for Osteogenesis

Macro- or Microfiber Scaffolds with Different Fiber Arrangements: Determining the Best Design for Osteogenesis

Specialized spatially-arranged non-circular fibers enhance filtration performance of African shrimp (Atya gabonensis).

African shrimp (Atya gabonensis) inhabit clear freshwaters, where the notably low concentration of food may pose a challenge to the efficacy of filter fibers on the chela for filter-feeding. Here, we investigate how the distinctive cross-sectional characteristics and spatial arrangement of the African shrimp's non-circular fibers contribute to the enhanced filtration performance of these specialized fibers. The unilateral thickening of the wall along the long axis of the elliptical cross-section of African shrimp fibers markedly enhances the filtration performance. The staggered and twisted arrangement of the fibers optimizes the surrounding flow field, achieving a favorable balance between pressure drop and collection efficiency, consequently improving their filtration performance in collecting fine particles (diameter: 2-10μm). Moreover, the arrangement of the fibers substantially increases the effective flow-facing filtering area of the fiber bundles, thus facilitating their efficiency in collecting larger particles (diameter > 10μm). The unique fiber properties of the African shrimp offer novel insights for the design and optimization of new fiber-filtering robots, presenting a wide range of potential applications, such as marine in-situ resource extraction, medical filtration, and industrial filtration.

Repair effect of different doses of human umbilical cord mesenchymal stem cells on white matter injury in neonatal rats

To compare the repair effects of different doses of human umbilical cord mesenchymal stem cells (hUC-MSCs) on white matter injury (WMI) in neonatal rats. Two-day-old Sprague-Dawley neonatal rats were randomly divided into five groups: sham operation group, WMI group, and hUC-MSCs groups (low dose, medium dose, and high dose), with 24 rats in each group. Twenty-four hours after successful establishment of the neonatal rat white matter injury model, the WMI group was injected with sterile PBS via the lateral ventricle, while the hUC-MSCs groups received injections of hUC-MSCs at different doses. At 14 and 21 days post-modeling, hematoxylin and eosin staining was used to observe pathological changes in the tissues around the lateral ventricles. Real-time quantitative polymerase chain reaction was used to detect the quantitative expression of myelin basic protein (MBP) and glial fibrillary acidic protein (GFAP) mRNA in the brain tissue. Immunohistochemistry was employed to observe the expression levels of GFAP and neuron-specific nuclear protein (NeuN) in the tissues around the lateral ventricles. TUNEL staining was used to observe cell apoptosis in the tissues around the lateral ventricles. At 21 days post-modeling, the Morris water maze test was used to observe the spatial learning and memory capabilities of the neonatal rats. At 14 and 21 days post-modeling, numerous cells with nuclear shrinkage and rupture, as well as disordered arrangement of nerve fibers, were observed in the tissues around the lateral ventricles of the WMI group and the low dose group. Compared with the WMI group, the medium and high dose groups showed alleviated pathological changes; the arrangement of nerve fibers in the medium dose group was relatively more orderly compared with the high dose group. Compared with the WMI group, there was no significant difference in the expression levels of MBP and GFAP mRNA in the low dose group (P>0.05), while the expression levels of MBP mRNA increased and GFAP mRNA decreased in the medium and high dose groups. The expression level of MBP mRNA in the medium dose group was higher than that in the high dose group, and the expression level of GFAP mRNA in the medium dose group was lower than that in the high dose group (P<0.05). Compared with the WMI group, there was no significant difference in the protein expression of GFAP and NeuN in the low dose group (P>0.05), while the expression of NeuN protein increased and GFAP protein decreased in the medium and high dose groups. The expression of NeuN protein in the medium dose group was higher than that in the high dose group, and the expression of GFAP protein in the medium dose group was lower than that in the high dose group (P<0.05). Compared with the WMI group, there was no significant difference in the number of apoptotic cells in the low dose group (P>0.05), while the number of apoptotic cells in the medium and high dose groups was less than that in the WMI group, and the number of apoptotic cells in the medium dose group was less than that in the high dose group (P<0.05). Compared with the WMI group, there was no significant difference in the escape latency time in the low dose group (P>0.05); starting from the third day of the latency period, the escape latency time in the medium dose group was less than that in the WMI group (P<0.05). The medium and high dose groups crossed the platform more times than the WMI group (P<0.05). Low dose hUC-MSCs may yield unsatisfactory repair effects on WMI in neonatal rats, while medium and high doses of hUC-MSCs have significant repair effects, with the medium dose demonstrating superior efficacy.

A micromechanical study on shear performance of unidirectional composites with randomly distributed fibers in the transverse cross‐section

AbstractThe properties of unidirectional (UD) composites has been investigated by analyzing their elastic and strength characteristics under shear loading. The UD fiber‐reinforced polymer (FRP) composites usually consist of fibers randomly dispersed throughout the cross‐section in the transverse direction which is generally not accounted for. A micromechanical model based on finite element analysis (FEA) approach is utilized here to examine these properties. The study begins by identifying micro scale unit cells or repetitive unit cells (RUCs) at the micro scale, as well as representative volume elements (RVEs). The distribution of fiber volume fraction (Vf) varies among various micro scale unit cells positioned in the transverse cross‐section. The elastic properties and strength are estimated by considering the properties of the matrix and fibers, and the configuration of micro scale unit cells, which feature a fiber arrangement which is categorized as hexagonal and varying Vf at different locations (from 0.35 to 0.75). However, the overall Vf for the UD composite remains constant at 0.65. Studies are also carried out with the random distribution of fibers at micro scale unit cells. The findings reveal that the random distribution of fibers significantly impacts the shear behavior of the composite. This investigation provides valuable insights into how the mechanical properties of the UD composite are subjective by the distribution of fiber, thereby contributing to the design and development of this light weight high‐performance material.Highlights Elastic and strength analysis of UD composites is presented. Emphasis is on fiber dispersion in transverse plane. Microscale unit cells, RUCs and RVE are identified. Studies are carried out based on micromechanical model. Influence of fiber distribution on shear behavior is investigated.

Load-Carrying Capacity of Thin-Walled Composite Columns with Rectangular Cross-Section under Axial Compression.

The aim of the current study was to determine the load capacity of composite columns subjected to axial compressive load. The subjects of the study were two types of columns with a rectangular cross-section, with different edge lengths. The tested columns had a closed cross-section. Four different fiber arrangements were analyzed for both cross-sections studied. The research was realized using interdisciplinary methods to determine the mechanism of damage to the composite material, with particular emphasis on damage initiation and propagation. Experimental tests were realized on a testing machine, the analysis was carried out with an acoustic emission system, and image analysis using visual assessment system of deflections of the walls of the structure. In addition, a number of numerical analyses were realized based on advanced modeling techniques for fiber-reinforced composites. A comparative analysis of both quantitative and qualitative results is presented for both analyses. The innovation of the presented research lies in the development of a custom method for modeling structures made of composite material with special emphasis on the failure phase. This will allow to accurately reflect the modeling of thin-walled structures with closed cross-section subjected to loading in a complex stress state.

Effect of poly-L-lactic acid and polydioxanone biostimulators on type I and III collagen biosynthesis.

Safe, effective, and biocompatible minimally invasive procedures with the potential to stimulate collagen production have been made to recover dermal thickness and skin quality. The main of this animal model experiment was to observe the effect of poly-L-lactic acid (PLLA) and polydioxanone (PDO) biostimulators in collagen I and III after hypodermal injection. Sixteen adult female rats (Wistar) were randomized into four groups and had dorsal treatment with: G1: hypodermic subcision (HS) only; G2: HS and PLLA hypodermic injection (HI), G3: HS and PDO HI; G4: Control, with no treatment. In histochemical, it was observed hypodermal and dermal tissue in more organized thickness in G3 and in G4 when compared to G1 and G2. There was few difference in G1 compared to G4. The tissue of G2 showed irregularities in the arrangement of collagen fibers, less defined structure and lower distribution of type I collagen compared to the other groups. There is a greater tendency for the proportions of type III collagen among tissues treated with both biostimulators (G2 and G3). PLLA and PDO had relatively similar percentages of collagen when compared to G4. The amount of type I collagen was higher in tissues treated with subcision, while type III collagen was higher in tissues treated with both biostimulators. G3 showed better performance in collagen production, although small, when compared with G2.

Experimental and numerical investigation on the elastic properties of luffa–cenosphere‐reinforced epoxy hybrid composite

AbstractEstimating the elastic characteristics of natural fiber‐reinforced polymer composites such as luffa fiber reinforced with epoxy is challenging. The structure of luffa cylindrica is complex, like a three‐dimensional natural fibrous mat, netting‐like structure. The multiscale modeling of such structures is the challenge to be addressed. The prime objective of this work is to determine the specific elastic properties of luffa–cenosphere‐reinforced epoxy (LCE) composite, considering the effect of filler volume fractions. Furthermore, multiscale modeling techniques, such as representative volume elements (RVEs) of finite element techniques with chopped, unidirectional, plain, and twill weaving fiber arrangements, were employed. The longitudinal modulus, transverse modulus, shear modulus, and Poisson's ratio were predicted through these modeling approaches. However, experimental and analytical methodologies, including the rule of mixture and Halpin–Tsai, were considered to validate the finite element analysis results. The elastic characteristics of LCE composite were therefore shown to be enhanced by increasing filler volume fraction. However, the cenosphere's 20% volume fraction has the highest elastic properties as determined by analytical, experimental, and computational models. Analytical and finite element simulation results were compared with the experimental results, and based on the findings, the most suitable (unidirectional, chopped, plain, and twill weaving) RVE was identified for finite element modeling of LCE composite for the evaluation of elastic properties. Results from practical approaches and the RVE twill weaving model showed good agreement, with less than 1% error, compared to the other analytical and finite element methods.Highlights NFCs are gaining ground in polymer composites. Overcoming challenges in modeling of luffa fiber inside epoxy matrix. The study uses multiscale modeling with diverse fiber arrangements. Experimental and analytical methods used to confirm FEA results. Increased cenosphere volume fraction boosts LCE composite properties.

Botulinum toxin type A inhibits M1 macrophage polarization by deactivation of JAK2/STAT1 and IκB/NFκB pathway and contributes to scar alleviation in aseptic skin wound healing

The non-neuronal and non-muscular effects of botulinum toxin type A (BTXA) on scar reduction has been discovered. This study was designed to investigate the effects of BTXA on macrophages polarization during the early stage of skin repair. A skin defect model was established on the dorsal skin of SD rats. BTXA was intracutaneous injected into the edge of wound immediately as the model was established. Histological examinations were performed on scar samples. Raw 264.7 was selected as the cell model of recruited circulating macrophages, and was induced for M1 polarization by LPS. Identify the signaling pathways that primarily regulated M1 polarization and respond to BTXA treatment. Application of BTXA at early stage of injury significantly reduced the scar diameter without delaying wound closure. BTXA treatment improved fiber proliferation and arrangement, and inhibited angiogenesis in scar granular tissue. The number of M1 macrophages and the levels of pro-inflammation were decreased after treated with BTXA in scar tissues. LPS activated JAK2/STAT1 and IκB/NFκB pathways were downregulated by BTXA, as well as LPS induced M1 polarization. At early stage of skin wound healing, injection of BTXA effectively reduced the number of M1 macrophages and the levels of pro-inflammatory mediators which contributes to scar alleviation. BTXA resisted the M1 polarization of macrophages induced by LPS via deactivating the JAK2/STAT1 and IκB/NFκB pathways.

Thermal properties of a raw earth-flax fibers building material

The aim of this paper is to investigate the thermal behavior of composite building materials comprised of silt and flax fibers, considering various initial conditions. The experiments were conducted on flax fibers alone, examining factors such as moisture content variation, fibers arrangement, and density. Subsequently, the thermal properties of the earth-flax fiber composite were studied, taking into account the influence of water content and fibers mass content. The thermal properties of both flax fibers and raw earth-flax fibers composite specimens were determined using the experimental unsteady-state method. The results indicate that the thermal conductivity and heat capacity of flax fibers increase with density, temperature, and moisture content. Regardless of fibers density and arrangement of fibers, linear relationships have been established for both thermal conductivity and heat capacity as functions of temperature. Additionally, it was demonstrated that the heat capacity and the thermal conductivity increase with water content, regardless of the percentage of added fibers. Moreover, mixing randomly flax fibers with compacted raw earth material has improved the thermal properties of the composite. This improvement pertains to its insulating capacity, ability to store heat, and, subsequently, its contribution to the thermal comfort provided by such earth composite material when used as a building material.

Study on moisture absorption characteristics of glass fibre‐reinforced epoxy resin material for composite insulators based on the 3D‐Fick model

AbstractLong‐term exposure to moisture leads to a gradual deterioration of performance and reduced service life of glass fibre‐reinforced epoxy resin (GFRP) material in composite insulators. Therefore, it is necessary to analyse the moisture absorption characteristics of GFRP material and the evolution of damage to their internal interface properties. Moisture absorption tests on GFRP rod material used in composite insulators to obtain their three‐dimensional diffusion coefficients are conducted. Atomic force microscopy was then employed to obtain the composite material system's fibre/matrix interfacial phase parameters. Furthermore, a finite element model incorporating representative volume elements with interfacial phases and a mesoscale transient moisture absorption finite element model for the composite material was established. Finally, the moisture absorption characteristics of GFRP material and the evolution of damage to the interfacial phase under thermal‐humidity cycling conditions were investigated. The results showed that the diffusion coefficient along the fibre direction in GFRP material was higher than that in the perpendicular direction. The moisture diffusion finite element model, incorporating an anisotropic interfacial phase, fitted the anisotropic diffusion coefficients of GFRP material more accurately. As moisture invaded the GFRP material, the mismatch stresses continuously increased during the moisture absorption. Moreover, the non‐uniform arrangement of fibres resulted in uneven distribution of moisture‐induced stresses inside the material, leading to higher mismatch stresses in areas with dense fibre arrangements in the matrix. Prolonged high and low humidity cycles led to the development of micro‐cracks, micro‐porosity, and interface debonding along the fibre direction at the GFRP material interfaces, thereby affecting the anisotropic moisture absorption characteristics of the material. The findings of this study provide valuable insights into the mechanisms underlying the deterioration of GFRP material in composite insulator rods due to moisture degradation.

Tooth acellular extrinsic fibre cementum incremental lines in humans are formed by parallel branched Sharpey’s fibres and not by its mineral phase

In humans, the growth pattern of the acellular extrinsic fibre cementum (AEFC) has been useful to estimate the age-at-death. However, the structural organization behind such a pattern remains poorly understood. In this study tooth cementum from seven individuals from a Mexican modern skeletal series were analyzed with the aim of unveiling the AEFC collagenous and mineral structure using multimodal imaging approaches. The organization of collagen fibres was first determined using: light microscopy, transmission electron microscopy (TEM), electron tomography, and plasma FIB scanning electron microscopy (PFIB-SEM) tomography. The mineral properties were then investigated using: synchrotron small-angle X-ray scattering (SAXS) for T-parameter (correlation length between mineral particles); synchrotron X-ray diffraction (XRD) for L-parameter (mineral crystalline domain size estimation), alignment parameter (crystals preferred orientation) and lattice parameters a and c; as well as synchrotron X-ray fluorescence for spatial distribution of calcium, phosphorus and zinc. Results show that Sharpey’s fibres branched out fibres that cover and uncover other collagen bundles forming aligned arched structures that are joined by these same fibres but in a parallel fashion. The parallel fibres are not set as a continuum on the same plane and when they are superimposed project the AEFC incremental lines due to the collagen birefringence. The orientation of the apatite crystallites is subject to the arrangement of the collagen fibres, and the obtained parameter values along with the elemental distribution maps, revealed this mineral tissue as relatively homogeneous. Therefore, no intrinsic characteristics of the mineral phase could be associated with the alternating AEFC incremental pattern.

(030) Hand Held, Self-Applicable Radio Frequency Device for the Treatment of Erectile Dysfunction. A Pilot Study with 6 Months Follow-Up

Abstract Introduction Collagen is an essential component of the structures involved in the erectile mechanism. Radiofrequency (RF) energy has been shown to renew and restore the spatial structural arrangement of collagen fibers and may improve erectile function of men suffering from erectile dysfunction (ED). Objective To assess the feasibility, safety, and efficacy of low-intensity radiofrequency (RF) therapy in men with ED. Methods Men with moderate &amp; mild to moderate ED, with or without the use of PDE5-inhibitors were included. After instructions, each patient treated himself with the radiofrequency device (Vertica®), applying his selected individual RF intensity. Patients underwent, according to the protocol, 12 sessions of 30-minute treatments over 2 months. Patients were evaluated at baseline, 1, 3 and 6 months after treatment using validated questionnaires; International Index of Erectile Function (Full IIEF), IIEF-EF, and erection Hardness score (EHS), satisfaction (EDITS, BSW), and disease-specific quality and quality of life (QEQ, SQOL). In each session, patients filled safety and side-effect questions (pain, fever, burning, discomfort, urinary disorders). The Wilcoxon signed ranks test and the Marginal Homogeneity test were used for statistical processing. Results Twenty-seven out of 28 men treated, were available for 6 months follow-up, mean age 59.2±9.7. The mean ED duration was 41.2±29 months. Mean baseline IIEF-EF was 16.8±1 and improved significantly to 24.8±1.6, 24.3±1.8, and 24.2±2.08 at 1, 3 and 6 months respectively (p&amp;lt;0.001). The Minimal Clinical Important Difference (MCID) criteria were met by 25/28 (89%) at 1-month follow-up and 20/27(74%) at 6-month follow-up (p&amp;lt;0.001). A statistically and clinically significant improvement was demonstrated in the Full IIEF and EHS scores at baseline and during follow-up. No correlation was found between IIEF improvement and the age of the patients. Borderline significance (p=0.05) was found between the duration of ED and IIEF-EF improvement. No side effects were reported and participants rated the device as very comfortable and easy to operate. Conclusions The novel radiofrequency, home-use device for the treatment of ED shows promising results and the potential to improve erectile function. In this study, it was feasible and safe. Further high-quality randomized controlled trials are needed to determine the long-term efficacy of the treatment. Disclosure Yes, this is sponsored by industry/sponsor: Ohhmed, Israel. Clarification: No industry support in study design or execution. Any of the authors act as a consultant, employee or shareholder of an industry for: Ohhmed, Freya, Besins, Verility.

Acoustically enhanced porous media enables dramatic improvements in filtration performance

Indoor air pollution affects millions of people worldwide. Conventional air purification techniques struggle to balance between safety, power consumption, efficacy, and cost. Acoustics have previously been leveraged to improve air purification with fiber filters, but little is known about how the arrangement of the fibers influences the acoustic effects, particularly for non-agglomerative purification performance relevant to fine and ultrafine pollutants. Here, we explore performance enhancements to fiber filters by applying external standing acoustic waves. We investigate the effects of the porous media arrangement in ordered (aligned and staggered) and disordered fiber media by building a multiphysics model, incorporating thermos-viscous acoustics, laminar flow, and particle tracing. Particles are modeled as point forces to isolate acoustic radiation forces and streaming. Attractive acoustic radiation forces dominate in the porous domain, leading to increased particle/boundary interactions. We find that the average acoustic radiation forces scale linearly with the average scattered acoustic pressure by the fiber medium. The scattered pressure varies depending on the fiber arrangement, indicating that there is an optimal design to increase the performance of acoustic-induced effects. We show a dramatic increase in performance efficiency, up to two orders of magnitude (123 times) for a staggered domain. This work has important applications to further advance acoustically enhanced filtration as a compelling solution for micro/nanoparticle separation processes.

Structural parameters defining distribution of collagen fiber directions in human carotid arteries

Collagen fiber arrangement is decisive for constitutive description of anisotropic mechanical response of arterial wall. In this study, their orientation in human common carotid artery was investigated using polarized light microscopy and an automated algorithm giving more than 4·106 fiber angles per slice. In total 113 slices acquired from 18 arteries taken from 14 cadavers were used for fiber orientation in the circumferential-axial plane. All histograms were approximated with unimodal von Mises distribution to evaluate dominant direction of fibers and their concentration parameter. 10 specimens were analyzed also in circumferential-radial and axial-radial planes (2-4 slices per specimen in each plane); the portion of radially oriented fibers was found insignificant. In the circumferential-axial plane, most specimens showed a pronounced unimodal distribution with angle to circumferential direction μ = 0.7° ± 9.4° and concentration parameter b = 3.4 ± 1.9. Suitability of the unimodal fit was confirmed by high values of coefficient of determination (mean R2 = 0.97, median R2 = 0.99). Differences between media and adventitia layers were not found statistically significant. The results are directly applicable as structural parameters in the GOH constitutive model of arterial wall if the postulated two fiber families are unified into one with circumferential orientation.

Shear wave propagation in a fiber-laden viscoelastic waveguide under prestress: Inverse modeling challenges

The functional role of skeletal muscle and the hierarchal microstructure and arrangement of fibers within it results in anisotropy and inhomogeneity in both material properties and imposed stresses. Dynamic elastography reconstruction methods for estimating muscle tissue viscoelastic properties that are based on assumptions of homogeneity, isotropy and only bulk wave motion may produce inaccurate estimates. Biases may be introduced in reconstruction by homogenizing muscle with axially aligned fibers and approximating it as transversely isotropic. The significance of these biases, and their interplay with imposed stresses and confounding waveguide effects due to small cross-sectional dimensions, is quantified with a series of numerical finite element and experimental elastography studies on cylindrically-shaped fiber-laden muscle phantoms, with varying fiber dimensions. Specifically, numerical simulations of elastography are conducted on 4-, 60- and 113-fiber models with the aligned fiber cross-sectional area fixed (reduced fiber diameter as fiber count increases) and comprising approximately 40% of the cross-sectional area of the cylindrical phantom that is also undergoing tensile axial pre-loading. Fiber elastic moduli twice that of connective tissue are considered. Experimental studies of the 4-fiber model are used to validate the numerical model. [Funding support: NSF 1852691 and NIH AR071162]

Enhancement of Wound Healing and Angiogenesis Using Mouse Embryo Fibroblasts Loaded in Decellularized Skin Scaffold.

Synthetic and natural polymer scaffolds can be used to design wound dressing for repairing the damaged skin tissue. This study investigated acute wound healing process using a decellularized skin scaffold and mouse embryo fibroblast (MEF). Mouse skin fragments were decellularized and evaluated by DNA content, toxicity, H&E staining, Raman confocal microscopy, Masson’s trichrome staining, SEM, and biodegradation assays. The fragments were recellularized by the MEFs, and cell attachment and penetration were studied. De- and decellularized scaffolds were used wound dressings in mouse acute wound models as two experimental groups. Using morphological and immunohistochemical assessments, wound healing was evaluated and compared among the experimental and control groups. DNA content of the decellularized tissue significantly reduced compared to the native control group (7% vs. 100%; p < 0.05). extracellular matrix components, e.g. collagen types I, III, and IV, elastin, and glycosaminoglycan, were well preserved in the decellularized group. The porosity and fiber arrangement in the stroma had a structure similar to normal skin tissue. A significant reduction in healing time was observed in the group treated with a decellularized scaffold. A thicker epidermis layer was observed in the recovered tissue in both experimental groups compared to the control group. Immunostaining showed a positive reaction for CD31 as an endothelial marker in both experimental groups, confirming new vascularization in these groups. Using MEFs with decellularized skin as a wound dressing increases the rate of wound healing and also the formation of new capillaries. This system could be beneficial for clinical applications in the field of tissue engineering.

Can the Sterilization Protocol Be Improved to Enhance the Healing of Allograft Tendons? An In Vivo Study in Rabbit Tendons.

Peracetic acid and irradiation are common sterilization methods for allograft tendons; however, under some conditions, both methods adversely affect the fiber arrangement and ultimate load of the tendon. An in vitro study showed that low-dose peracetic acid combined with irradiation may be less detrimental to allograft tendon structure and properties, possibly because the breakdown of peracetic acid can lead to an enlargement of the interstitial spaces and an increase in porosity. Using a rabbit Achilles tendon model, we asked: What is the effect of peracetic acid-ethanol combined irradiation on (1) the histopathology and fiber diameter of the allograft tendon, (2) tensile creep and load-to-failure biomechanical properties of allograft tendons, and (3) healing of the treated tendon in vivo compared with fresh-frozen allograft and peracetic acid-ethanol sterilization at 4 and 8 weeks? The Achilles tendons used in this study were sourced from euthanized 10-week-old male New Zealand White rabbits previously used for ophthalmic experiments. All allografts were divided into three groups: fresh-frozen group (control group, n = 20), peracetic acid-ethanol sterilization group (n =20), and peracetic acid-ethanol combined irradiation group (n = 20). The sterilization protocols were performed per a predetermined plan. In the peracetic acid-ethanol sterilization group, the tendon tissues were covered with the peracetic acid-ethanol sterilization solution (1% peracetic acid for 30 minutes). In the peracetic acid-ethanol combined irradiation group, the tendon tissues were covered with the peracetic acid-ethanol sterilization solution (0.2% peracetic acid for 30 minutes) and were subjected to 15 kGy gamma irradiation. Thirty 10-week-old male New Zealand White rabbits received bilateral Achilles tendon allografts surgically. Tendon samples from each group were harvested at 4 weeks (n = 30) and 8 weeks (n = 30) postoperatively. For each timepoint, eight tissues were used for histologic staining and electron microscopy, 15 tissues were used for biomechanical testing, and seven tissues were used for hydroxyproline assay and quantitative polymerase chain reaction. Histopathology was determined qualitatively by hematoxylin and eosin and Masson staining, while fiber diameter was measured quantitatively by transmission electron microscopy. Biomechanical properties were measured using cyclic loading tests and load-to-failure tests. The healing outcome was quantitatively judged through healing-related genes and proteins. At 4 weeks and 8 weeks postoperatively, the peracetic acid-ethanol combined irradiation group visually demonstrated the best continuity and minimal peripheral adhesions. Histologic staining showed that tendon fibers in the peracetic acid-ethanol combined irradiation group maintained consistent alignment without notable disruptions or discontinuities, and there was a qualitatively observed increase in the number of infiltrating cells compared with the control group at the 4-week timepoint (444 ± 49 /mm 2 versus 256 ± 43 /mm 2 , mean difference 188 /mm 2 [95% confidence interval 96 to 281]; p < 0.001). At 8 weeks postoperatively, the tendon fiber diameter in the peracetic acid-ethanol combined irradiation groups was similar to that of the control group (0.23 ± 0.04 µm versus 0.21 ± 0.03 µm, mean difference 0.02 µm [95% CI -0.04 to 0.08]; p = 0.56). At 8 weeks postoperatively, the peracetic acid-ethanol combined irradiation group exhibited better properties in terms of both ultimate load (129 ± 15 N versus 89 ± 20 N, mean difference 40 N [95% CI 7 to 73]; p = 0.02) and energy absorption density (17 ± 6 kJ/m 2 versus 8 ± 4 kJ/m 2 , mean difference 8 kJ/m 2 [95% CI 0.7 to 16]; p = 0.004) compared with the control group. Gene expression analysis revealed higher expression levels of COL1A1 (2.1 ± 0.8 versus 1.0 ± 0, mean difference 1.1 [95% CI 0.1 to 2.1]; p = 0.003) and MMP13 (2.0 ± 0.8 versus 1.0 ± 0, mean difference 1.0 [95% CI 0.4 to 1.6]; p = 0.03) in the peracetic acid-ethanol combined irradiation group than in the control group. There was a higher amount of collagen Type I in tendons treated with peracetic acid-ethanol combined irradiation than in the control group (0.36 ± 0.03 versus 0.31 ± 0.04, mean difference 0.05 [95% CI 0.01 to 0.09]; p = 0.02). Treatment with peracetic acid-ethanol combined irradiation did not have any discernible adverse effect on the histology, fiber diameter, enzymatic resistance, collagen content, or biomechanical strength of the allograft tendons compared with the control group. Peracetic acid-ethanol combined irradiation treatment had a positive impact on remodeling of the extracellular matrix and realignment of collagen fibers. This sterilization method could be helpful to expand the scope and frequency with which allogeneic materials are applied. The long-term healing effect and strength of allograft tendons must be tested before clinical use, and it is necessary to conduct comparative studies on autografts and synthetic materials that are currently widely used clinically.

Influence of layering sequence on performance of jute/wool epoxy hybrid composites: a comparative study with automotive plastic

Natural fibres such as Jute and Wool are increasingly being used in fibre reinforced polymer composites, hence progressively supplanting synthetic fibres. Combining these two unique natural fibers would improve the performance of composites used in secondary structural applications. This study is intended to investigate the effects of Jute and Wool fiber arrangement on mechanical properties in an epoxy matrix. In order to achieve this, hand layup was employed to fabricate composite laminates consisting of four layered fabric layers stacked in sequences, as Jute/Wool/Wool/Jute (JW1), Wool/Jute/Jute/Wool (JW2), Jute/Wool/Jute/Wool (JW3), and Jute/Jute/Wool/Wool (JW4). Effect of these stacking sequences on the properties of Natural Fibre Hybrid Composites (NFHCs) was evaluated by conducting a series of standardized tests such as Flexural, Interlaminar Shear Strength (ILSS), Impact and Single End Notch Bend (SENB) tests, as per ASTM standards. On the other hand an automobile interior thermoplastic material was evaluated for different mechanical properties and compared with hybrid Jute-Wool composite. Experimental results showed that the JW1 composite, exhibited superior mechanical properties because it has tailored with a Wool fabric at the core and Jute fabric on its outer surface. Hence, JW1 had a remarkable tensile strength of 40 MPa, a maximum flexural strength of 99 MPa and extremely high interlaminar shear strength (ILSS) of 3.09 MPa. In addition to this, JW1 also had high fracture toughness, measuring around 72 M P a m 1 /2, and impressive impact strength of 85 J/m2. Furthermore, scanning electron microscope (SEM) photo images confirmed that the presence of strong bonding between the fibres and matrix in JW1 composite exhibited and overall improvement of mechanical performance by 82% when compared with other combinations (JW2, JW3, and JW4) including automotive plastics.