Arrangement Of Fibers Research Articles

Study of the influence of macro-structure and micro-structure on the mechanical properties of stag beetle upper jaw

Macrostructural control of stress distribution and microstructural influence on crack propagation is one of the strategies for obtaining high mechanical properties in stag beetle upper jaws. The maximum bending fracture force of the stag beetle upper jaw is approximately 154, 000 times the weight of the upper jaw. Here, we explore the macro and micro-structural characteristics of two stag beetle upper jaws and reveal the resulting differences in mechanical properties and enhancement mechanisms. At the macroscopic level, the elliptic and triangular cross-sections of the upper jaw of the two species of stag beetles have significant effects on the formation of cracks. The crack generated by the upper jaws with a triangular section grows slowly and deflects easily. At the microscopic level, the upper jaw of the two species is a chitin cross-layered structure, but the difference between the two adjacent fiber layers at 45° and 50° leads to different deflection paths of the cracks on the exoskeleton. The mechanical properties of the upper jaw of the two species of stag beetle were significantly different due to the interaction of macro-structure and micro-structure. In addition, a series of bionic samples with different cross-section geometries and different fiber cross angles were designed, and mechanical tests were carried out according to the macro-structure and micro-structure characteristics of the stag beetle upper jaw. The effects of cross-section geometry and fiber cross angle on the mechanical properties of bionic samples are compared and analyzed. This study provides new ideas for designing and optimizing highly loaded components in engineering. Statement of significanceThe upper jaw of the stag beetle is composed of a complex arrangement of chitin and protein fibers, providing both rigidity and flexibility. This structure is designed to withstand various mechanical stresses, including impacts and bending forces, encountered during its burrowing activities and interactions with its environment. The study of the upper jaw of the stag beetle can provide an efficient structural design for engineering components that are subjected to high loads. Understanding the relationship between structure and mechanical properties in the stag beetle upper jaw holds significant implications for biomimetic design and engineering.

Enhancing non-metallic gasket performance with shape memory fibers

This study aims to enhance the sealing performance of non-metallic gaskets by incorporating shape memory fibers. The analytical exploration focuses on analyzing the thermomechanical behavior of Nitinol fibers in their woven state within the gasket. Specifically, the investigation examines the variation of phase transformation zones across the wire cross-section with changes in temperature. Subsequently, a series of SMA fiber-reinforced gasket samples were fabricated, and experimental investigations were conducted to assess the impact of Nitinol fibers on composite gaskets using a helium gas leak test. The results demonstrate that the integration of shape memory alloy wires into the composition of gaskets can significantly improve their sealing performance. Moreover, this study examines a number of pivotal factors such as fiber volume fraction, temperature application, woven fiber arrangement, leakage rates, gasket deformation, contact stress, and structural bending. These comprehensive investigations may provide valuable insights into the efficacy of Nitinol fibers in enhancing gasket sealing performance.

Simulation-based Flexible Needle Control with Single-core FBG Feedback for Spinal Injections.

We present a general framework of simultaneous needle shape reconstruction and control input generation for robot-assisted spinal injection procedures, without continuous imaging feedback. System input-output mapping is generated with a real-time needle-tissue interaction simulation, and single-core FBG sensor readings are used as local needle shape feedback within the same simulation framework. FBG wavelength shifts due to temperature variation is removed by exploiting redundancy in fiber arrangement. Targeting experiments performed on both plastisol lumbar phantoms as well as an ex vivo porcine lumbar section achieved in-plane tip errors of and , and total tip errors of and for the two testing environments. Our clinically inspired control strategy and workflow is self-contained and not dependent on the modality of imaging guidance. The generalizability of the proposed approach can be applied to other needle-based interventions where medical imaging cannot be reliably utilized as part of a closed-loop control system for needle guidance.

The influence of membrane fiber arrangement on gas exchange in blood oxygenators: A combined numerical and experimental analysis

Oxygenators take over lung function in the event of severe lung injuries by exchanging gas into the blood stream using hollow fiber membrane mats. A central limitation of oxygenators is their large foreign surface area and priming volume, which cause blood damage.Thus, gas exchange efficiency needs to be improved through an understanding of the interaction between fiber arrangement and blood flow directions. This has only been investigated in two dimensional or simplified non-realistic fiber bundles. The aim of this study was to quantify gas exchange in realistic 3D fiber bundles.We performed three dimensional micro scale CFD simulations of different realistic fiber arrangements and flow directions that we validated in corresponding test oxygenators using porcine blood.Fiber-configurations influence gas transfer by factor 2, with highest transfer rates for blood flowing in circumferential direction, and lowest for longitudinal direction in wound oxygenators. The CFD model correlates with the experiments with an R2 = 0.88.For the first time, the configuration of realistic 3D fiber bundles was proven to strongly influence gas transfer in oxygenators, in simulations and experiments. The CFD model serves for investigation of further configurations and to derive transfer coefficients for full scale oxygenators.

Numerical and experimental study on anisotropic heat transfer behaviors of quartz fabric composite preforms: Multiple micro‐scale models method

AbstractThis paper presents a comprehensive study on anisotropic heat transmission behaviors in quartz fiber fabrics. Considering the random distribution characteristic of fibers within the yarn, an innovative two‐scale finite element method (tFEM) is introduced, incorporating multiple micro‐scale fiber models (MMFM) based on scanning electron microscope (SEM) observations. MMFM are divided into 8 distinct models to capture intricate fiber arrangements. The macro‐scale fabric model is constructed based on the geometric structure analysis by Micro‐CT technology. Both micro‐ and macro‐scale models are assembled with the air matrix to form the two‐phase composite model. The Hot‐Disk thermal analysis instrument is applied to measure the anisotropic thermal conductivity (ATC). Numerical results from MMFM shows more excellent agreements with the experimental ones at 3D orthogonal directions of fabrics, i.e., the error rates of the thermal conductivity in the warp, weft and thickness directions between numerical and experimental methods are all less than 3%, which indicates the tFEM including MMFM in this paper is more accurate than the tFEM including OSMFM. In addition, the temperature distribution and heat transmission differences due to fiber arrangements are simulated and illustrated through the MMFM. Afterwards, temperature drop and isothermal characteristics are demonstrated in this study.Highlights Effects of fiber arrangements on steady heat transfer behaviors at micro‐scale are illustrated. Isothermal and temperature‐drop characteristics at micro‐ and macro‐scale are simulated and studied. The transverse isotropy of thermal conductivity at micro‐scale and the anisotropy of that at macro‐scale are revealed.

Non-conformance aspects of moulded composite materials and “corresponding” simulation models with 3D textile reinforcement

Composite materials with 3-dimensional (3D) reinforcement were manufactured and corresponding simulation models were created in parallel. The used simulation approach has earlier been shown to produce close to authentic geometrical representation of the yarn architecture in 3D reinforcement. It is shown that although the as-woven reinforcement pattern can be modelled quite reliably, significant distortion from the nominal fibre arrangement might take place later in manufacturing, primarily related to compression during moulding. Such effects have earlier received significant attention for composites with 2-dimensional reinforcement but not as much for their 3D counterparts. The yarns in the real and the simulated materials are studied and compared, and some of the discrepancies and the mechanisms behind are discussed. The distortions are partly attributed to the relatively sparse weave that allows yarns oriented in the through-thickness direction, in particular, to deviate from their original positions.

Traumatic Rosette cataract: A case report

Rosette cataract is characterized by a star-shaped opacity that forms in the lens of the eye after blunt or penetrating trauma. Traumatic cataracts constitute a significant portion of ocular injuries, with rosette cataract being a distinctive subtype. Understanding its formation and progression is critical for effective management. Blunt trauma to the eye causes a shock wave that disrupts the lens fibres. This disruption leads to the characteristic rosette pattern due to peculiar lens fibres arrangement. Patients may present with blurred vision, glare, and photophobia due to associated traumatic iritis. The rosette pattern may be visible upon slit-lamp examination. In early stages, observation and protective eyewear may be recommended. Significant visual impairment due to rosette cataract will require cataract surgery. Either small incision cataract surgery or Phacoemulsification with intraocular lens (IOL) implantation is the standard treatment for advanced cases. Timing of surgery depends on the degree of visual impairment and patient’s needs.

Effect of Polycarbosilane (PCS) on the High-Temperature Properties of Phenolic Resin (Phen) Films and Quartz Fiber/Phen-PCS Composites

In this study phenolic resin (Phen)-loaded PCS (Phen-PCS) films were prepared and quartz fiber/Phen-PCS composites based on the resin films were also prepared. The quartz fiber/Phen-PCS composites were prepared by impregnating 2.5 Dimension (2.5D was an arrangement of fibers) quartz fibers using the resin film infusion (RFI) process. The properties of the composites were investigated. The study demonstrated that the density of the composites, after curing gradually increased with the increase in PCS. The flexural strength of the composites exhibited a trend of increasing and then decreasing after curing and pyrolysis at 1100 °C under an N2 atmosphere. The flexural strength of the composites reached 13.73 MPa when the addition of PCS was 30% of the phenolic resin, representing an increase of 74.23% compared to that of the composites without PCS. The porosity was found to be as low as 2.3% after curing and 2.1% after pyrolysis. X-ray diffraction (XRD) analysis and infrared spectroscopy tests demonstrated that ceramicization products, such as SiO2 and SiC, were produced after pyrolysis of the composites containing PCS. This transformation significantly enhanced the ablation resistance of the composites. Butane flame tests were performed, and the lowest line and mass ablation rates were 0.026 mm/s and 0.196 mg/s after curing. The results of the study revealed the potential of PCS as a precursor to improve the mechanical properties and heat resistance of composites, which offers promising material options for high-temperature applications.

Effect of filter microstructure on filtration characteristics in a nonwoven bag filter: A resolved CFD-DEM approach coordinated with X-ray computed tomography image

A resolved CFD-DEM was used to simulate aerosol filtration through the microstructure of nonwoven bag filter media. The filtration behavior was simulated for three filter domains with different fiber orientations obtained from X-ray CT images of a commercial bag filter. Minimal particles were collected in the filter domain with fibers oriented parallel to the main flow. However, in the filter domain with fibers oriented perpendicularly, the particles were collected by the fibers and formed a cake layer after blocking the flow path. To verify this trend, simulations were performed using a model filter in which the fiber arrangement was systematically varied. The simulation results revealed that to collect particles and form a cake layer the inter-surface distance between fibers must be smaller than the particle diameter and the fiber orientation should be perpendicular. The sieving effect is essential for particles to remain within the filter.

Assessment of the effects of the use of preconsumer cotton waste on the quality of rotor yarns

Due to the shortage of raw fiber materials and stricter legislative conditions, it has become necessary to process even the more contaminated fiber wastes that contain only a small amount of useable fibers. Hence, this paper investigates the impact of different cleaning channels on the Rieter R37 rotor spinning machine in controlling spinning strategy and yarn quality using recovered blowroom cotton waste fibers. The study involves spinning 98-tex yarns with different cleaning channels during the spinning process, using recovered blowroom cotton waste in blends with virgin cotton processed into slivers by two methods. Qualitative indicators in the fiber-sliver-yarn line were evaluated and graphically compared. The statistical significance of the influencing factors was determined using the Generalized Anova. Additionally, the quality of the experimental samples was compared to authentic data from global production using the USTER® STATISTIC. The results underline the significant influence of fiber quality, sliver preparation method and implemented cleaning channels on the arrangement of fibers in yarn structures as evidenced by structural and mechanical parameters. In particular, the comparison with USTER® STATISTICS confirms that all yarns meet the required quality standards for selected applications, including those spun exclusively from 100 % cotton waste. Furthermore, the results demonstrate that this innovative technology enables yarn manufacturers to meet customer demands, ensure optimal yarn quality and achieve cost savings by optimizing waste removal without compromising fiber yield.

Designing and simulating fiber arrangement in the probe of elastic scattering signal to be more efficient

Designing and simulating fiber arrangement in the probe of elastic scattering signal to be more efficient

Oriented cellulose hydrogel: Directed tissue regeneration for reducing corneal leukoplakia and managing fungal corneal ulcers

Fungal corneal ulcer is one of the leading causes of corneal blindness in developing countries. Corneal scars such as leukoplakia are formed due to inflammation, oxidative stress and non-directed repair, which seriously affect the patients' subsequent visual and life quality. In this study, drawing inspiration from the oriented structure of collagen fibers within the corneal stroma, we first proposed the directional arrangement of CuTA-CMHT hydrogel system at micro and macro scales based on the 3D printing extrusion method combined with secondary patterning. It played an antifungal role and induced oriented repair in therapy of fungal corneal ulcer. The results showed that it effectively inhibited Candida albicans, Aspergillus Niger, Fusarium sapropelum, which mainly affects TNF, NF-kappa B, and HIF-1 signaling pathways, achieving effective antifungal functions. More importantly, the fibroblasts interacted with extracellular matrix (ECM) of corneal stroma through formation of focal adhesions, promoted the proliferation and directional migration of cells in vitro, induced the directional alignment of collagen fibers and corneal stromal orthogonally oriented repair in vivo. This process is mainly associated with MYLK, MYL9, and ITGA3 molecules. Furthermore, the downregulation the growth factors TGF-β and PDGF-β inhibits myofibroblast development and reduces scar-type ECM production, thereby reducing corneal leukoplakia. It also activates the PI3K-AKT signaling pathway, promoting corneal healing. In conclusion, the oriented CuTA-CMHT hydrogel system mimics the orthogonal arrangement of collagen fibers, inhibits inflammation, eliminates reactive oxygen species, and reduces corneal leukoplakia, which is of great significance in the treatment of fungal corneal ulcer and is expected to write a new chapter in corneal tissue engineering.

Quantified planar collagen distribution in healthy and degenerative mitral valve: biomechanical and clinical implications

Degenerative mitral valve disease is a common valvular disease with two arguably distinct phenotypes: fibroelastic deficiency and Barlow’s disease. These phenotypes significantly alter the microstructures of the leaflets, particularly the collagen fibers, which are the main mechanical load carriers. The predominant method of investigation is histological sections. However, the sections are cut transmurally and provide a lateral view of the microstructure of the leaflet, while the mechanics and function are determined by the planar arrangement of the collagen fibers. This study, for the first time, quantitatively examined planar collagen distribution quantitatively in health and disease using second harmonic generation microscopy throughout the thickness of the mitral valve leaflets. Twenty diseased samples from eighteen patients and six control samples were included in this study. Healthy tissue had highly aligned collagen fibers. In fibroelastic deficiency they are less aligned and in Barlow’s disease they are completely dispersed. In both diseases, collagen fibers have two preferred orientations, which, in contrast to the almost constant one orientation in healthy tissues, also vary across the thickness. The results indicate altered in vivo mechanical stresses and strains on the mitral valve leaflets as a result of disease-related collagen remodeling, which in turn triggers further remodeling.

Identification of interfacial strength between carbon fiber and epoxy matrix in CFRP laminates using pulse laser spallation method

Carbon fiber reinforced plastics (CFRP) have high specific strength and specific stiffness and are used in various fields such as aircraft, space structures, automobiles, and other transportation equipment. Since the internal structure of CFRP is very complex, it is very difficult to define failure criteria when external loads are applied. Various methods have been proposed to evaluate the interfacial shear strength between carbon fiber and resin matrix, such as microbond test, rupture test, and pull-out test, but currently no effective method has been proposed to evaluate the tensile strength of the CF/resin interface. In this study, we applied the pulsed laser spallation method, which has been confirmed to be effective for evaluating the adhesion strength of thin films and coating films on substrates, to the evaluation of CFRP. A finite element method based on the Laplace transform was used for the numerical analysis of the unsteady elastodynamic problem. The macroscopic stresses generated by ultrasonic waves excited by pulsed laser irradiation were calculated by inverse analysis using a transfer function with the displacement history of the back surface of the CFRP specimen as supplementary information. Microscopic stresses at the fiber/resin interface were evaluated by the homogenization method, taking into account the effects of fiber arrangement, distance between fibers, and molding residual stress in unidirectionally reinforced CFRP laminates. As a result, this series of investigations revealed that the vertical interfacial stress is dominant with respect to the strength of the fiber/resin interface with the strength of about 54MPa.

How crack twisting in bouligand structures lead to damage delocalization and toughening

Fiber-reinforced composites with Bouligand structure exhibit remarkable mechanical properties due to the intricate arrangement of fibers. In this study, we propose a coarse-graining (CG) model specifically developed to capture the behavior of Bouligand structures. The model incorporates bonded interactions to represent the fibers and employs a double-well potential to describe the non-bonded interactions within the matrix. Using this model, we investigate the fracture mechanics properties of Bouligand structures, with a particular focus on the emergence of helicoidal cracks. Our primary objective is to validate the hypothesis that these twisting cracks, which align with the fiber orientation, contribute to local hardening mechanisms. By hindering the growth of individual cracks, these hardening mechanisms facilitate the nucleation and growth of multiple cracks, thereby promoting a delocalization effect within the material. Through extensive simulations and analysis, we confirm the validity of our hypothesis. The presence of twisting cracks indeed induces local hardening mechanisms, making it more challenging for individual cracks to propagate. This phenomenon effectively spreads the damage, dissipating energy across larger volumes of the material. Consequently, the toughness of these Bouligand structures is enhanced, as this delocalization effect effectively mitigates the concentration of damage. These findings provide valuable insights into the fracture behavior of Bouligand structures and shed light into the underlying mechanisms responsible for their exceptional mechanical performance. Moreover, our CG model offers a practical and efficient approach to studying and understanding the fracture mechanics properties of complex fiber-reinforced composites. The ability to simulate and analyze the behavior of helicoidal cracks within Bouligand structures opens up new avenues for designing and optimizing advanced materials with enhanced toughness and damage resistance.

Effect of Consumption of Caffeinated Energy Drinks on the Myocardium of the Adult Male Albino Rat (A Histological and Morphometric Study)

Abstract Background Energy drinks are non-alcoholic beverages containing mainly caffeine. Chronic consumption of energy drinks may be associated with increased blood pressure, and cardiac work load, as well as, increased heart rate. Energy drinks consumption has been related to myocardial infarction in healthy young adult. Aim of the Work The aim of the present work was to study the harmful effect of energy drinks histologically and morphometrically, on the myocardium of the adult male albino rats. Material and Methods 20 Adult male albino rats aging 4-6 moths were divided randomly into two groups; Group I (Control group) which consisted of 10 male albino rats with free access to food (rat chew) and water and Group II (Experimental group): 10 male albino rats which received 3.75 ml/kg body weight red bull through oral gavage daily for 4 weeks with free access to food (rat chew) and water. At the end of experiment, rats werescarificed, and cardiac muscle was extracted and processed into paraffin blocks for light microscopic examination. Morphometric study and statistical analysis were done. Results The present work demonstrated that Red Bull induced several histopathological changes of the cardiac muscle. The results showed the cardiac muscle of rats with irregularities in the arrangement of fibers and wide spaces in between. Some cardiomyocytes showed areas of pale stained sarcoplasm, partial loss of the cross striations and ill-defined intercalated discs. Localized areas of hyaline degeneration and areas of tissue disruption were also noticed with less frequently localized coalesced cytoplasmic vacuoles. The nuclei of the cardiomyocytes appeared small, darkly stained, and pyknotic. Others were flattened and deeply stained. Congested, and thick walled blood vessels were also observed, with mononuclear cellular infiltrations. In Masson’s Trichrome sections, relative increase in collagen deposition between the cardiac muscle fibers was observed which was associated with focal fibrous degeneration. Examination of the semithin sections showed partial disruption of the cardiomyocytes. The nuclei of the cardiomyocytes were small, darkly stained with perinuclear degeneration. Some nuclei were eccentric, or with an irregular outline, and peripheral chromatin condensation. The characteristic finding was the presence of longitudinal wavy cardiac muscle fibers with relatively wide spaces in between and localized areas of partially lost cross striations and intercalated discs. Dilated, thick walled blood vessels was noticed between the bundles of cardiac muscle. Mononuclear inflammatory cellular infiltrations were also detected. Conclusion The present study demonstrated the degenerative and cardiotoxic effects of Red Bull as an example of energy drinks on the histological structure of the cardiac muscle.

Effect of Zanthoxylum bungeanum extract on the quality and cathepsin L activity of Niuganba

Niuganba (NGB) is a traditional fermented beef product. Protease activity typically significantly affects the quality of NGB. Some natural food extracts may markedly influence NGB's protease activity and performance. This study aims to investigate the effect of Zanthoxylum bungeanum extract (ZBE) on the quality and cathepsin L activity of NGB. Following ZBE treatment, the myofibril fragmentation index (MFI), the content of TCA-soluble peptides, surface hydrophobicity, disulfide bond content, and cathepsin L activity of NGB significantly decrease. The content of free thiol groups and β-sheet significantly increases. Scanning electron microscopy (SEM) reveals that the arrangement of muscle fibers in the cross-section of NGB is more compact after ZBE treatment. The research results indicate that ZBE effectively inhibits cathepsin L activity, alleviates the degradation of myofibrillar proteins, improves the physicochemical characteristics of NGB, and enhances its structural stability.

Shapes and terminal velocities of single bubbles rising through fiber bundle in stagnant water

The shapes and terminal velocities of single bubbles in the surface tension force and the inertial force dominant regime were measured in a vertical bundle consisting of fluorinated ethylene propylene fibers of 2 mm diameter. Three kinds of fiber arrangements were tested with the fiber pitches Lp of 18, 12, and 6 mm. The terminal velocities in the bundles were larger than those in the absence of a fiber bundle and increased with decreasing Lp. The bubble shapes were significantly deformed by fibers, which would be the cause of the velocity increase, i.e., a reduction in the form drag. The terminal velocities in the bundles were well predicted by the combination of the drag correlation in the absence of a fiber bundle and the multiplier for bubbles in fiber bundles.

Emergence of high-mass stars in complex fiber networks (EMERGE)

Context. Recent molecular surveys have revealed the rich gas organization of sonic-like filaments at small scales (so-called fibers) in all types of environments prior to the formation of low- and high-mass stars. These fibers form at the end of the turbulent cascade and are identified as the fine substructure within the hierarchical nature of the gas in the interstellar medium (ISM). Aims. Isolated fibers provide the subsonic conditions for the formation of low-mass stars. This paper introduces the Emergence of high-mass stars in complex fiber networks (EMERGE) project, which investigates whether complex fiber arrangements (networks) can also explain the origin of high-mass stars and clusters. Methods. We analyzed the EMERGE Early ALMA Survey including seven star-forming regions in Orion (OMC-1,2,3, and 4 South, LDN 1641N, NGC 2023, and the Flame Nebula) that were homogeneously surveyed in three molecular lines (N2H+ J = 1–0, HNC J = 1–0, and HC3N J = 10–9) and in the 3 mm continuum using a combination of interferometric ALMA mosaics and IRAM-30 m single-dish (SD) maps, together with a series of Herschel, Spitzer, and WISE archival data. We also developed a systematic data reduction framework allowing the massive data processing of ALMA observations. Results. We obtained independent continuum maps and spectral cubes for all our targets and molecular lines at different (SD and interferometric) resolutions, and we explored multiple data combination techniques. Based on our low-resolution (SD) observations (30″ or ~12 000 au), we describe the global properties of our sample, which covers a wide range of physical conditions, including low-(OMC-4 South and NGC 2023), intermediate (OMC-2, OMC-3, and LDN 1641N), and high-mass (OMC-1 and Flame Nebula) star-forming regions in different evolutionary stages. The comparison between our single-dish maps and ancillary YSO catalogs denotes N2H+ (1–0) as the best proxy for the dense, star-forming gas in our targets, which show a constant star formation efficiency and a fast time evolution of ≲1 Myr. While apparently clumpy and filamentary in our SD data, all targets show a much more complex fibrous substructure at the enhanced resolution of our combined ALMA+IRAM-30 m maps (4″.5 or ~2000 au). A large number of filamentary features at subparsec scales are clearly recognized in the high-density gas (≳ 105 cm−3) that is traced by N2H+ (1–0) directly connected to the formation of individual protostars. Surprisingly, this complex gas organization appears to extend farther into the more diffuse gas (~103−104 cm−3) traced by HNC (1–0). Conclusions. This paper presents the EMERGE Early ALMA Survey, which includes a first data release of continuum maps and spectral products for this project that are to be analysed in future papers of this series. A first look at these results illustrates the need of advanced data combination techniques between high-resolution interferometric (ALMA) and high-sensitivity, low-resolution single-dish (IRAM-30 m) datasets to investigate the intrinsic multiscale, gas structure of the ISM.

Isorhamnetin inhibits hypertrophic scar formation through TGF-β1/Smad and TGF-β1/CREB3L1 signaling pathways

BackgroundHypertrophic scar (HS) is a common fibrotic skin disease that occurs secondary to burns or injuries. The activation of the TGF-β signaling pathway contributes immensely to HS formation. Isorhamnetin (ISO) is a type of flavonoid compound that exerts an antifibrotic effect via TGF-β signaling suppression. However, whether ISO can inhibit HS formation via TGF-β signaling is yet to be elucidated. This study aimed to examine the influence of ISO on HS pathogenesis and TGF-β signaling, especially the downstream molecules and networks of TGF-β signaling that facilitate HS formation. MethodsHypertrophic scar fibroblasts (HSFBs) were isolated from human HS tissues. The in vitro proliferation, migration, contractile ability, cell cycle, and apoptosis of HSFBs after ISO treatment were determined using cell viability assay, EdU staining, wound healing assay, collagen gel contraction assay, and flow cytometry. The expressions of genes and proteins involved in TGF-β signaling and its downstream molecules in ISO-treated HSFBs were determined using quantitative PCR (qPCR), immunofluorescence, and western blotting. In vivo, a rabbit HS model was established, and the effects of ISO on rabbit HS formation were investigated using histological analysis, immunohistochemical staining, and qPCR. ResultsIn vitro studies indicated that ISO treatment suppressed the proliferation, migration, and contractile ability of HSFBs; attenuated the expressions of COL Ⅰ, COL Ⅲ, and α-SMA; and inhibited TGF-β1 signaling-induced activation of HSFBs by decreasing the levels of phosphorylated Smad2/3 and cleaved CREB3L1 in a dose-dependent manner. Furthermore, ISO augmented apoptosis and G2 phase cell cycle arrest of HSFBs by upregulating the expressions of the proapoptotic proteins Bax and cleaved caspase-3 and downregulating the expression of the antiapoptotic protein Bcl-2. In vivo studies revealed that ISO ameliorated HS formation in the rabbit ear by lowering the scar elevation index, attenuating the collagen density, facilitating the regular arrangement of collagen fibers, and downregulating the expressions of TGF-β1, CREB3L1, COL Ⅰ, COL Ⅲ, and α-SMA. ConclusionsISO suppressed HS pathogenesis by dampening TGF-β1/Smad and TGF-β1/CREB3L1 signaling pathways, which suggests that it may serve as a candidate inhibitor of TGF-β1 signaling and a promising anti-HS drug with a high therapeutic potential.