Based on theoretical analysis and simulation software, the paper researched the performance of transmission after using tilt-Spectral fiber Bragg grating for SSB modulation in Radio over Fiber system. The study concluded that DSB signal can be converted to SSB signal by using tilt-Spectral fiber Bragg grating, and inhibit the carrier power. There are two ideal conditions for a best grating. First, one side of DSB signal will be completely filtered, while the other side remains the same power. Secondly, carrier power equals to sideband power in SSB signal, which means that carrier-to-sideband ratio is 0dB. As the result, the best tilt-Spectral fiber Bragg grating is designed. Transmitting the SSB signal which is filtered by the best grating in optical fiber not only eliminates the phenomenon that output amplitude change with the transmission distance cyclically, it also enhances the transmission efficiency and receiver sensitivity.