In this study an approach was made to efficaciously synthesize gold decorated mulberry fibers by a facetious dip coating procedure. Assortments of inorganic compounds are being employed to impart antimicrobial properties to fabrics and textiles. Earlier workers have used gold as coloring agent for cotton and yarn etc. Herein, for the first time an attempt was made to liftoff a progressive textile conjured of gold and mulberry fibers which will be exploited for fabrication of antimicrobial stuffs for diverse medical and non-medical uses. The morphology, physicochemical and antibacterial characteristics of gold decorated mulberry fibers were scanned via X-ray diffraction (XRD), Scanning electron microscope (SEM), Electron probe microanalysis (EPMA), Fourier transform infrared (FTIR) and antibacterial testing. The average size of gold particles was between 1.9 and 3 μm. The even distribution of Au on mulberry fibers was corroborated by SEM, and the results of XRD and FTIR analysis confirmed integration of nanogold in mulberry. The XRD data of Au blended fibers revealed no change in peak location, implying that gold coating has no effect on mulberry fiber structure. Gold decorated mulberry has a little higher intensity and a slight change in peak position toward higher wavenumber, which could be related to functionalization or interaction of gold particles with mulberry fibers. These gold incapacitated mulberry fibers shown out antibacterial activity against illustrative bacterium E. coli. Thus our study provides optimistic indications to pick up mulberry fibers decked with nanogold as prime material for fabrication of forthcoming antimicrobial materials for therapeutic and non-therapeutic tenacities.