Fertilizer Treatments Research Articles

Enhancing Roselle Growth and Yield through Planting Density and Fertilization Strategies: A Study on BRIS Soil Area

Optimizing planting density and fertilization practices are crucial factors influencing the micro-environment of agricultural fields, thereby profoundly impacting the growth, development, and yield formation of Roselle plants. This study investigates the combined effects of planting density and Sesbania grandiflora integration on nitrogen dynamics within the BRIS soil area, aiming to enhance Roselle growth and yield. Employing a randomized complete block design (RCBD), three fertilizer treatments—control, green manure, and organic fertilizer—were implemented. Nitrogen content was analyzed using the Kjeldahl method, while phosphorus and potassium contents were determined via UV spectrometry and atomic absorption spectrophotometry, respectively. Within an agroforestry model employing a spacing of 20 cm between plants, results revealed that Roselle plants treated with organic fertilizer exhibited higher nitrogen (N: 3.54 %), phosphorus (P: 0.54 %), and potassium (K: 1.61 %) content in their leaves, and similarly in their roots (N: 1.93%, P: 0.45%, K: 2.11%). However, soil analysis indicated that green manure fertilizer treatment led to higher nitrogen (0.11 %), phosphorus (44.67 %), and potassium (0.17 %) content within the BRIS soil. In conclusion, the green manure fertilizer treatment demonstrated a propensity to enhance nitrogen dynamics within the poorly structured BRIS soil, thereby promoting growth and development. Conversely, the integration of Sesbania grandiflora along with suitable plant spacing appeared instrumental in sustaining Roselle yield productivity. This research sheds light on the interplay between planting density, fertilizer strategies, and soil dynamics, providing valuable insights for optimizing Roselle cultivation practices in similar agroecological contexts.

Pertumbuhan dan Produksi Tanaman Selada (Lactuca sativa L.) yang Diaplikasi Kompos Padat dengan Pupuk Urea

Lettuce is one of the vegetable plants that contain water, energy, protein, and fat; rich in carbohydrates, fiber, sugar, calcium, iron, magnesium, phosphorus, potassium, sodium, zinc, copper, manganese, selenium, vitamin C, and several other vitamins. Solids are the waste from the by-products of fresh fruit bunch (FFB) processing in palm oil mills. Inorganic fertilizers are fertilizers from chemical, physical, and biological engineering processes resulting from an industry or fertilizer manufacturing plant. The provision of solid compost with urea fertilizer is expected to complement each other's nutrient needs. In contrast, lettuce plants need nitrogen nutrients in the vegetative phase and protein formation in their tissues. This research aimed to evaluate the effect of several doses of solid compost treatment with urea fertilizer and get the best combination of solid compost with urea fertilizer on the growth and production of lettuce plants (Lactuca sativa L.). The research was conducted experimentally using a completely randomized design (CRD) with six fertilizer treatments. The treatments included: 30 ton/ha solid compost + 0 kg/ha urea fertilizer, 30 ton/ha solid compost + 100 kg/ha urea fertilizer, 30 ton/ha solid compost + 200 kg/ha urea fertilizer, 20 ton/ha solid compost + 0 kg/ha urea fertilizer, 20 ton/ha solid compost + 100 kg/ha urea fertilizer, 20 ton/ha solid compost + 200 kg/ha Urea fertilizer. The results were analyzed using variance analysis and continued with Duncan's multiple range test at the 5% level. Applying 20 - 30 tons/ha of solid compost with 200 kg/ha of urea fertilizer gave the best results on the growth and yield of lettuce plants.

A Bayesian approach to analyzing long-term agricultural experiments

Effective and flexible statistical analyses are key to getting the most out of long-term experiments (LTEs). Here, we aim to introduce Bayesian analysis to the wider LTE community and show how the modelling process differs from traditional statistical analyses. Bayesian methods have become increasingly popular due to more flexibility in model development with better access to statistical software and sampling algorithms. Using Bayes' Theorem, model coefficients are estimated by incorporating any prior knowledge we may have on model terms. Including prior knowledge in this way requires a different estimating procedure for a fitted model. Bayesian model coefficients are usually sampled from thousands of samples from one or more runs of a Markov Chain. We present the use of Bayesian analyses through three examples. Example 1 illustrates a single regression with and without factors using the Broadbalk Long-Term Experiment, showing how the estimated model changes with more uncertainty in our prior knowledge of model coefficients. Example 2 demonstrates the use of multiple regression, predicting grain yield from factor variables and seasonal weather variables. Example 3 shows an estimation of soil carbon changes under crop rotation and fertilization treatments with a hierarchical time series model using a Swedish soil fertility experiment.

Legal Case Study of Severe IVF Incidents Worldwide: Causes, Consequences, and High Emotional, Financial, and Reputational Costs to Patients and Providers

Background In light of the changing legislative and regulatory landscape concerning reproductive rights and fertility treatments worldwide and a growing number of patients who use assisted reproductive technology (ART)/in vitro fertilization (IVF) treatments to overcome their reproductive challenges (cancer patients, those needing genetic screening, members of the LGBTQIA+ community, and patients who have trouble conceiving naturally) and achieve their family-building goals, ART/IVF clinics face numerous operational and legal challenges, which come at great emotional, reputational, and financial costs to patients and providers. Objectives Fertility treatment related incidents and resulting legal cases vary in severity, scope, and outcomes. Local legal and regulatory environment for patients and ART/IVF providers increase the complexity. The authors aimed to identify the causes of lawsuits against ART/IVF providers and how legal outcomes varied between cases. Study design The data on U.S. IVF incidents was collected from Lexis Nexis, Westlaw, Bloomberg Law, and CaseLaw databases, newspaper and magazine articles, legal reviews, peer-reviewed journals, and online publications. For international cases, HFEA reports, and industry and online publications were queried. The searches were not time restrained but was carried out from January 2022 to April 2023. RESULTS Two hundred five IVF incidents (84.9% U.S., 2.9% U.K., 12.2% other), excluding large-scale tank and alarm failures and power disruptions, which affected 307 people (79.8%, 3.3%, 16.9%) and/or 258 specimens (84.5%, 2.7%, 12.8%), resulting in 76 lawsuits (65.8%, 7.89%, 26.3%) were identified worldwide, and categorized by error types. Specimen mix-ups were the most prevalent type (95.0%). Meanwhile, ten failed storage and alarm incidents caused most damage, affecting >1800 patients and >8100 specimens, and resulting in 181 initial lawsuits. CONCLUSIONS Overreliance on manual protocols, irregular/skipped audits, and human error were responsible for IVF incidents reviewed. Damaged, destroyed, or lost embryos and embryo transfer to the wrong recipient have lifelong devastating effects on patients, for many of whom IVF was their last chance for parenthood due to cancer treatment, infertility, and/or age. To complicate the cases, embryo mix-ups resulted in custody disputes over the newborn child(ren), as in Manukyan v. CHA Health Systems, and loss of identity in children and parents. U.S. babies born to embryo mix-ups are reunited with their genetic parents, following the legal precedent of Perry-Rogers v. Fasano. Many countries, however, grant the custody of the child(ren) to birth parents. Most lawsuits were dismissed or settled. The combination of changing abortion and personhood laws in several states of the US can further complicate the issue, as they might put patients and providers in legal jeopardy, following routine ART/IVF procedures. This may include preimplantation genetic testing, short- and long-term embryo storage, and embryo disposal. These state laws will limit patient options and restrict ART/IVF clinic operations. Owing to all these factors, in this study, we can only make educated estimates about the true scope of the issue and its financial cost to ART/IVF providers, which might range from thousands to millions of USD. Lawsuits also come at a great reputational cost for patients, their families and providers, so the latter might benefit from embracing digitization of records, automation, robotics, and AI as a standard of care in their practice.

Increasing Hybrid Rice Yield, Water Productivity, and Nitrogen Use Efficiency: Optimization Strategies for Irrigation and Fertilizer Management.

Water and fertilizer are crucial in rice growth, with irrigation and fertilizer management exhibiting synergies. In a two-year field study conducted in Yiyang City, Hunan Province, we examined the impact of three irrigation strategies-wet-shallow irrigation (W1), flooding irrigation (W2), and the "thin, shallow, wet, dry irrigation" method (W3)-in combination with distinct fertilizer treatments (labeled F1, F2, F3, and F4, with nitrogen application rates of 0, 180, 225, and 270 kg ha-1, respectively) on rice yield generation and water-fertilizer utilization patterns. The study employed Hybrid Rice Xin Xiang Liang you 1751 (XXLY1751) and Yue Liang you Mei Xiang Xin Zhan (YLYMXXZ) as representative rice cultivars. Key findings from the research include water, fertilizer, variety, and year treatments, which all significantly influenced the yield components of rice. Compared to W2, W1 in 2022 reduced the amount of irrigation water by 35.2%, resulting in a 42.0~42.8% increase in irrigation water productivity and a 25.7~25.9% increase in total water productivity. In 2023, similar improvements were seen. Specifically, compared with other treatments, the W1F3 treatment increased nitrogen uptake and harvest index by 1.4-7.7% and 5.9-7.7%, respectively. Phosphorus and potassium uptake also improved. The W1 treatment enhanced the uptake, accumulation, and translocation of nitrogen, phosphorus, and potassium nutrients throughout the rice growth cycle, increasing nutrient levels in the grains. When paired with the F3 fertilization approach, W1 treatment boosted yields and improved nutrient use efficiency. Consequently, combining W1 and F3 treatment emerged as this study's optimal water-fertilizer management approach. By harnessing the combined effects of water and fertilizer management, we can ensure efficient resource utilization and maximize the productive potential of rice.

Why no-till system sequesters more carbon and is more resilient and productive with contrasting fertilization regimes in a highly weathered soil?

Land management systems that comprise the principles of conservation agriculture (CA) can lead to soil organic carbon (SOC) gains over time. Nonetheless, how fertilization regimes interfere with their performance in highly weathered soils is still uncertain. This study presents results on SOC storage, crop yield, and soil resilience from a long-term experiment in southern Brazil (Ponta Grossa – Paraná State) 26 years after its establishment in 1989 combining a gradient of soil disturbance through diverse soil management strategies with contrasting fertilization regimes. We hypothesized that preserving soil structure rebuilt over time through no-till system plays a significant role in SOC persistence and the fertilization regime can impact land management performance on soil resilience and crop yield. The experimental design was laid out as a split plot through completely randomized blocks. The main plots comprised the treatments related to soil management systems: 1) conventional plow-based tillage – CT; 2) minimum tillage (Chiselling replacing plowing) – MT; 3) no-till with one chisel plowing every three years – NTch; and 4) continuous no-till system – NTS. The sub-plots comprised full crop fertilization (FCF) for all crops and low crop fertilization (LCF) by suppressing K and P fertilization and maintaining N in broadcast application. SOC stocks significantly improved as the soil disturbance diminished, resulting in higher soil resilience indexes for NTS and NTch. Differences in SOC stocks between the contrasting treatments NTS and CT were higher under low fertilization, resulting in C and N sequestration rates of 1.14 and 0.14 Mg ha−1 year−1 under LCF compared to 0.77 and 0.08 Mg ha−1 yr−1 in FCF at the 0–100 cm layer. Such higher differences were induced by overall higher SOC stocks of CT when under FCF and higher SOC stocks in subsoil depths promoted by NTS when under LCF. High fertilization treatments produced cumulative yields 1.5 times higher for soybeans and 2.5 times higher for corn throughout the 26 years of the experiment. Labile C fractions extracted by hot water (HWEOC) and K-permanganate (POXC) were systematically increased as the disturbance diminished. Gains in labile fractions were promoted in deeper layers in lower disturbance treatments (NTch and NTS). We conclude that combining conservation agriculture principles ultimately defined the potential for SOC sequestration. The high soil resilience under the NTS in this research indicates a considerable potential to reverse the soil degradation and decline of the SOC and labile fractions by conversion to intensive NTS (high and diversified annual C input) associated with absence of soil disturbance.

Impact of salinity and fertilization on soil properties, and root development in fenugreek (Trigonella foenum-graecum) cultivation

Salinity is a paramount factor that poses challenges to agricultural productivity and sustainability. At the same time, fenugreek is valued as a forage crop for its medicinal properties in addition to its extensive edible use. The objective of this study is to explore how fertilization and salinity impact soil physical properties and root system development in fenugreek cultivation. A field experiment was established at the Agricultural University of Athens during growing seasons 2019-2020 (1st GS) and 2020-2021 (2nd GS) in a split-splot design with the 2 main salinity treatments (High salinity; HS & Conventional salinity; CS) and 5 fertilization treatments (biocyclic-vegan (BHS), manure (FYM), compost (COMP), inorganic fertilization (11-15-15) and the control (C). Soil porosity was statistically significantly affected by both salinity (p<=0.05) and fertilization (p<=0.001). Also, organic matter was significantly affected by fertilization (p<=0.001). HS (59.78±2.65) resulted in 20.02% fewer nodules on plant roots compared to CS treatments (71.75±2.65). The maximum number of nodules was recorded in the FYM treatment (68.93±0.77). In addition, mean root diameter was affected by fertilization (p<=0.01) COMP (2.92±0.31 mm) and NPK treatments (2.83±0.31 mm) resulted in 19.52% and 23.32% smaller root diameter respectively compared to BHS, while FYM (2.68±0.31 mm) resulted in a 30.22% smaller diameter. A significant increase of seed yield was noticed under organic fertilization where the highest yield of 2.1 t ha−1 was recorded in BHS (2nd GS). Although fenugreek was affected by high salinity, it demonstrated considerable resistance and maintained its yields, rendering it a crop suitable for challenging soils.

Effects of Long-Term Fertilizer Managements on Soil Organic Carbon Mineralization Under the Double-Cropping Rice System in Southern China

ABSTRACT Soil organic carbon (SOC) plays an important role in maintaining or increasing soil fertility and quality in paddy field, but there is limited information about how SOC mineralization responds to different fertilizer managements under the double-cropping rice system in southern China. Therefore, this study was designed to explore changes in SOC content, soil enzyme activities, SOC mineralization at 0–10 cm and 10–20 cm layers, and its relationship with long-term fertilizer managements in a double-cropping rice system of southern China. The experiment included four fertilizer treatments: chemical fertilizer alone (CF), rice straw and chemical fertilizer (RS+F), 30% organic manure and 70% chemical fertilizer (OM+F), and without fertilizer input as a control (Con). These results showed that SOC and labile organic carbon contents at 0–10 cm and 10–20 cm layers in paddy field with RS+F and OM+F treatments were increased, compared to CF and Con treatments. Compared with Con treatment, SOC mineralization rate and accumulation with CF, RS+F, and OM+F treatments were increased. SOC accumulation and potential mineralization at 0–10 cm and 10–20 cm layers with RS+F and OM+F treatments were increased, compared with CF and Con treatments. SOC mineralization rate and accumulation at 0–10 cm layer with CF, RS+F, OM+F, and Con treatments were higher than that of 10–20 cm layer. These results indicated that soil invertase, cellulose and urease activities in paddy field with RS+F and OM+F treatments were significantly higher than that of CF and Con treatments. There was a significant positive relationship between SOC accumulation and SOC content, soil invertase, cellulose, urease activities but were significant negative correlated with soil pH, bulk density. As a result, these were beneficial practices for improving SOC content and SOC mineralization in a double-cropping rice field of southern China by combined application of rice residue or organic matter with chemical fertilizer managements.

Navigating water and nitrogen practices for sustainable wheat production by model-based optimization management systems: A case study of China and Pakistan

Water and fertilizer losses have often affected wheat yield per unit area in China and Pakistan. The 2-year field experiments were conducted at six research stations. The experimental sites in China were Tongzhou, Qiliying and Yuanyang. In Pakistan were Faialsaabad, TandoJam and Dokri. Fertilizer treatments were: N275, N207 and N135 kg ha−1 in Tongzhou and N125, N105 and N90 kg ha−1 in Faisalabad. While the irrigation treatments were: W210–230, W160–190 and W110–140 mm ha−1 in Qiyliying, W350, W305 and W250 mm ha−1 in Yuanyang, W435, W400 and W365 mm ha−1 in TandoJam and W380, W340 and W300 mm ha−1 in Dokri. The grain yields, water and N losses were simulated using the WHCNS model. The model showed a good ability to capture the effects of management practices and read the environmental diversity. The study revealed that major contributors to water and N losses were N leaching (r=0.98), evaporation and drainage (r=0.77–0.96) rates. In Tongzhou, the N207 treatment maintained yield, NUE and decreased N loss. In Faisalabad, the N125 increased yield but led to higher N loss and reduced NUE. Furthermore, the decline in surface soil water content was a common issue in Pakistani sites and wheat yields were 27.48–41.60 % lower than in China. In Qiliying, the W160–190 and W110–140 and in Yuanyang, the W300 and W250 treatments decreased the annual average water loss by 33.49–12.38 mm and 66.06–55.01 mm, respectively. In TandoJam, the W400 and W365 and in Dokri, the W380 and W340 treatments decreased water loss by 72.34–45.87 mm and 56.84–37.82 mm compared to the full irrigation amount. Overall, results showed that 17.39–23.81 % of water use could be decreased without yield penalty in Qiliying and 14.29 % in Yuanyang. In Pakistani sites of TandoJam and Dokri, a decreased irrigation amount of 10.5 % is recommended.

Effect of Vermicompost on Soil Fertility and Crop Productivity in the Drylands of Ethiopia

The nutrient type and amount contributed from vermicompost varies depending on the source material, earthworm type used, agro-ecology and farmers’ management. These call for crop, soil and site specific study. This study, therefore, aimed at determining: (i) the optimal vermicompost application rate/s for wheat and maize production; and (ii) the role of vermicompost on soil fertility improvements. Field experiments in three agro-climatic zones (highland, midland and lowlands) were established on wheat and maize crops following a randomized complete block design. The treatments were vermicompost (2.5, 5.0, 7.5 and 10 t ha−1), conventional compost (10 t ha−1), and recommended rates of Nitrogen and Phosphorus (NP = 100 kg DAP and 50 kg Urea) fertilizers. Our results revealed that the studied soil chemical properties, primarily, organic carbon (OC %), N (%) and available P (mg kg−1) increased with increasing vermicompost rate. The 10 t ha−1 vermicompost treated plots had 157-210%, 64-81% and 100-242% higher soil total nitrogen content as compared to the control, 10 t ha−1 conventional compost and NP fertilizer treatments, respectively. Application of 10 t ha−1 vermicompost also resulted in a 1.5-fold and 43-63% grain yield increment of both tested crops compared to the control and NP treated plots, respectively. However, the highest net benefit was obtained from 5 t ha−1 vermicompost for maize (86% increase) and 10 t ha−1 of vermicompost for wheat (152% increase compared to the control). In conclusion, vermicompost at 5 t ha−1 for maize and 10 t ha−1 for wheat can be recommended to sustainably manage farm productivity.

Recent Advances in Resource Utilization of Huangshui from Baijiu Production

Huangshui is a typical organic wastewater in Chinese Baijiu production, with high pollution and valuable ingredients. Conventional wastewater treatment leads to resource-wasting and environmental pollution. It is urgent that the demand for effective Huangshui treatment with the development of the Baijiu-making industry. This review systematically summarizes recent studies, revealing the main characteristics and application of Huangshui, focusing on the application of the rich microbial resources and flavor substances, which provides a practical approach to cascade and full use of Huangshui in medicine, cosmetic, functional food, fertilizer, and wastewater treatment fields. Further research suggested that Huangshui can also be used as an external carbon source for the denitrification system or as an organic liquid water-soluble fertilizer for more fruits and grains. The applications favor improving production efficiency and lowering pollutant emissions and introduce novel concepts for the sustainable development of related industries. Thus, Chinese Baijiu plants can achieve the near-zero emissions of wastewater and cleaner production.

Integrating bio-organic fertilization increases twice-yearly cabbage crop production by modulating soil microbial community and biochemical properties in Northwest Plateau

Decreasing the reliance on chemical fertilizers and integrating bio-organic fertilizers are effective strategies for enhancing soil quality, promoting sustainable agricultural development, and protecting the environment. However, their impact on cabbage (Brassica oleracea L. var. capitata) cultivation in the Northwest Plateau remains unclear. The study investigated the impact of reducing chemical fertilizer by 30 %, combined with different amounts of bio-organic fertilizer (3000 kg·ha−1 for T1, 6000 kg·ha−1 for T2, and 12000 kg·ha−1 for T3), were examined in comparison to a conventional local fertilizer treatment (CK2), which served as the control. Our findings demonstrated significant improvements in soil properties and enzyme activities for T1, T2, and T3 treatments compared to CK2. Specifically, the contents of soil organic matter (SOM), available potassium (AK) and available phosphorus (AP) increased significantly by 10.06 %, 10.63 % and 18.33 %, respectively. Meanwhile, accompanied by a decrease in electrical conductivity (EC) values. Soil sucrase and urease activities can be enhanced by 10.43 % and 19.61 %, respectively. Redundancy analysis confirmed that SOM, AP, AK, and EC were the primary factors driving fluctuations in the microbial community structure. The addition of bio-organic fertilizer led to an increase in beneficial soil microorganisms. The abundance of Proteobacteria and Actinobacteria increased most strongly by 4.24 % and 8.75 %. The microbial community suggested that these beneficial microorganisms played essential roles in the cycling of nutrients, suppression of pest and disease, and the promotion of robust plant growth. Moreover, the addition of bio-organic fertilizer (T3) significantly improved plant growth, root/shoot ratio, and nutrient content, and increased the yield by 6.78 t·ha−1. Therefore, we suggest that a reduction in the use of chemical fertilizers along with the incorporation of bio-organic fertilizer, is an important measure for improving soil fertility, enriching soil microbial communities, and ensuring the safety of crops and the environment in the Northwest Plateau.

Effects of Straw Addition on N2O and CO2 Emissions from Red Soil Subjected to Different Long-Term Fertilization

Straw return, as an important measure for soil fertility improvement in farmland, significantly affects the emissions of greenhouse gases N2O and CO2. Thus, the collected soil samples from five long-term (30-year) fertilization treatments (no fertilization, CK; recommended chemical fertilizer, F; 200 % of recommended chemical fertilizer, 2F; pig manure, M; and chemical fertilizer combined with pig manure, FM) were amended with and without straw and incubated under constant temperature and humidity conditions (25 ℃ and 65 % maximum field water holding capacity) for 20 days so as to investigate the key factors influencing N2O and CO2 emissions in response to straw addition in long-term fertilization treatments. The results showed that fertilization significantly increased N2O emissions. Compared to those under the unfertilized treatment[(22.05 ±2.09) μg·kg-1, calculated as nitrogen, the same as below], cumulative N2O emissions from the chemical fertilizer treatments significantly increased by 119 %-195 %[(48.38 ±20.81) μg·kg-1 and (65.13 ±12.55) μg·kg-1 from the F and 2F treatments, respectively], and those from the manure treatments increased by 275 %-399 %[(82.72 ±12.73) μg·kg-1 and (1 101.99 ±425.71) μg·kg-1 from the M and FM treatments, respectively]. Soil NO3--N, DOC, and DTN were the main factors influencing N2O emissions from fertilized treatments in the absence of straw addition. Straw addition significantly increased cumulative N2O emissions by 345 % and 247 % in the 2F and M treatments, respectively, compared to those in the corresponding fertilized treatments without straw addition, with no significant effect on N2O emissions in the CK, F, and FM treatments. Straw addition increased DOC content and microbial activity and decreased soil NO3--N and DTN contents, thereby increasing N2O emissions. Fertilization also significantly increased CO2 emissions. Compared to those from the unfertilized treatment, cumulative CO2 emissions from the manure treatments significantly increased by 120 %-130 %[(122.11 ±4.3) mg·kg-1 (calculated as carbon, the same as below) and (116.47 ±4.55) mg·kg-1 from the M and FM treatments, respectively], and those in the 2F treatment increased by 28 %[(65.13 ±12.55) mg·kg-1]. In the absence of straw addition, soil MBC, DOC, and DTN were the main factors influencing CO2 emissions. Compared to those in the treatments without straw addition, straw addition significantly increased cumulative CO2 emissions by 660 %-1132 % among fertilization treatments, due to increased DOC and MBC contents and enhanced microbial activity. In conclusion, straw addition significantly increased N2O and CO2 emissions through increased soil DTN consumption and DOC content among fertilization treatments. In soils treated with manure amendment, straw return should be rationally considered for the purpose of balancing the comprehensive trade-offs between fertility improvement and greenhouse gas emissions.

Effect of Partial Substitution of Chemical Fertilizers with Organic Fertilizers on N2O and NO Emissions from a Peach Orchard

Organic fertilizer substitution has been promoted as a weight loss, efficient, and diversified fertilizer substitution technology in agricultural production. However, there is a lack of comprehensive assessment of the impact of organic fertilizers on N2O and NO emissions from orchards. In this study, N2O and NO emissions from peach orchards were observed annually using static dark box-gas chromatography to compare the effects of chemical fertilizer application alone and partial replacement of chemical fertilizer treatment on NO emissions from peach orchards. The results showed that the partial replacement of chemical fertilizers with organic fertilizers reduced the total N2O and NO emissions from peach orchards by 15.0 % and 9.4 %, respectively. The N2O and NO emission factors were reduced by 21.3 % and 21.1 %. The mineral N content of the soil in the organic fertilizer treatment was lower than that in the chemical fertilizer treatment alone. The organic fertilizer treatment increased the contribution of AOA to nitrification and decreased the contribution of AOB, thus reducing N2O and NO from nitrification. In addition, the results of the dual isotope mixing model[δ18O(N2O/H2O) vs. δ15NSP] indicated that the bacterial denitrification/nitrifying bacterial denitrification (bD/nD) process served as the primary pathway for N2O emissions in peach orchards. Partial substitution with organic fertilizers enhanced soil denitrification, resulting in larger reductions in the amounts of N2O and NO. Therefore, partial substitution of organic fertilizer is a viable measure to mitigate nitrogen oxide emissions from orchards and to achieve green and low-carbon development in agriculture.

Effects of Different Proportions of Ammonium Sulfate Replacing Urea on Soil Nutrients and Rhizosphere Microbial Communities

In order to investigate the effects of ammonium sulfate, an industrial by-product, on soil nutrients and microbial community when applied in different proportions instead of using urea as nitrogen fertilizer, a pot corn experiment was conducted. A completely randomized block experimental design was used, with a total of five treatments:CK (no fertilization), U10S0 (100 % urea), U8S2 (80 % urea + 20 % ammonium sulfate), U6S4(60 % urea + 40 % ammonium sulfate), and U0S10 (100 % ammonium sulfate). The basic physical and chemical properties of soil and the dry weight of maize plants were determined by conventional methods, and microbial sequencing was performed using the Illumina NovaSeq platform. The experiment results showed that:① In each growth stage of maize, the pH of soil treated with fertilization (7.85-8.15) was decreased compared with that of CK (8.1-8.21), and the pH showed a decreasing trend with the increase in ammonium sulfate content. ② The soil available nitrogen content increased gradually with the increase in the ammonium sulfate ratio at each growth stage of maize. Compared with that in the CK and U10S0 treatments, the ratio in the U0S10 treatment increased 30.56 % to 63.68 % and 13.22 % to 38.43 %, respectively. The variation trend of organic carbon content was opposite to that of available nitrogen (U8S2 > U6S4 > U0S10), and the addition of ammonium sulfate was still higher than that of U10S0 at other growth stages except for the seedling stage. ③ The protease activity of all fertilization treatments was higher than that of the control, and the protease activity was gradually enhanced with the continuous growth of corn and the increase in the ammonium sulfate ratio. The protease activity of the U0S10 treatment was higher than that of the U10S0 treatment at each growth stage of corn, which increased by 10.54 %-100 %. Soil sucrase activity ranged from 0.04 to 0.24 mg·(g·24 h)-1, and those in the U0S10 treatments were significantly higher than those in the U10S0 and CK treatments at all growth stages, increasing by 20.32 % to 99.16 % and 24.31 % to 79.33 %, respectively. ④ The species abundance of bacteria and fungi in maize rhizosphere under all fertilization treatments were lower than those under the CK treatment, followed by those under the U10S0 treatment. The species diversity trend of the bacterial community in the three treatments with ammonium sulfate replacing urea were U8S2 > U0S10 > U6S4, and that of fungi were U6S4 > U8S2 > U0S10. ⑤ The maize dry weight of the U10S0 treatment and U0S10 treatment was the highest, which was 39.47 % and 36.16 % higher than that of the CK treatment, respectively, but the difference was not significant. The Pearson model showed that the species abundance and diversity of soil rhizosphere fungi and bacteria were affected by relevant environmental variables, among which pH value and soil available nitrogen content were the most important factors affecting microbial diversity. In conclusion, when corn planting in calcareous brown soil, replacing urea with a certain proportion of ammonium sulfate can improve soil nutrients more than urea alone, which affects the growth and rhizosphere microbial community of corn to a certain extent and has a greater yield.

An Example of a New Approach, New Assessment, New Statistical Inference, and New Conclusions from the Published Research by Nikipelova, O., Pyliak, N., & Hodorchuk, V. (2023). Organic fertilizers in increase of hazelnut yield. Interdepartmental Thematic Scientific Collection of Phytosanitary Safety, (69), 118-128. https://doi.org/10.36495/1606-9773.2023.69.118-128.

Background: The declining availability of traditional organic fertilizers and the need for sustainable waste management solutions have sparked interest in alternative nutrient sources for agriculture. This study investigates the potential of sewage sludge-based biofertilizers in hazelnut (Corylus avellana L.) cultivation, addressing both agricultural productivity and environmental concerns. Objective: To evaluate and compare the effects of sewage sludge-based biofertilizers with traditional organic fertilizers on soil agrochemical properties and assess their environmental safety in hazelnut plantations in the Southern Steppe of Ukraine. Methods: A two-year field experiment (2021-2022) was conducted, comparing six treatments: control (no fertilizer), cattle manure, chicken manure, two formulations of sewage sludge-based biofertilizers, and pure sewage sludge. Soil samples were analyzed for easily hydrolyzable nitrogen, mobile phosphorus and potassium, organic matter content, and ecotoxicity. Advanced statistical analyses, including ANOVA, Tukey's post-hoc tests, paired t-tests, and correlation analyses, were employed to assess treatment effects and temporal changes. Results: Statistically significant differences were observed between fertilizer treatments for all major nutrients (p < 0.05). Sewage sludge-based Biofertilizer No. 1 demonstrated superior performance in increasing soil phosphorus content compared to traditional organic fertilizers (306.30 mg/kg vs. 174.75 mg/kg for cattle manure, p < 0.05). All organic fertilizers, including biofertilizers, significantly improved soil nitrogen content over the two-year period (p < 0.05). Potassium levels showed a significant increase (13.41% year-over-year, p < 0.05), while changes in nitrogen and phosphorus levels suggested positive trends but did not reach statistical significance. Importantly, ecotoxicity levels remained low (E < 1.5) across all treatments, indicating minimal short-term environmental risk. Conclusions: This study provides compelling evidence for the potential of sewage sludge-based biofertilizers as effective and environmentally safe alternatives to traditional organic fertilizers in hazelnut cultivation. The findings have significant implications for sustainable agriculture, waste management, and circular economy practices. However, long-term studies are recommended to fully assess the cumulative effects on soil health, crop productivity, and potential contaminant accumulation. This research contributes to the development of innovative, sustainable fertilization strategies that can address both agricultural productivity and environmental conservation needs.

Effects of Four Amendments on Cadmium Bioavailability and Enzyme Activity in Purple Soil

In this study, the effects of four types of amendments on effective Cd and Cd content in different parts of prickly ash soil and soil enzyme activity were studied, which provided scientific basis for acidification improvement of purple soil and heavy metal pollution control. A field experiment was conducted. Six treatments were set up:no fertilizer (CK), only chemical fertilizer (F), lime + chemical fertilizer (SF), organic fertilizer + chemical fertilizer (OM), biochar + chemical fertilizer (BF), and vinasse biomass ash + chemical fertilizer (JZ). Soil pH; available Cd (DTPA-Cd); Cd content in branches, leaves, shells, and seeds of Zanthoxylum; as well as the activities of catalase (S-CAT), acid phosphatase (S-ACP), and urease (S-UE) in different treatments were studied, and their relationships were clarified. The results showed following:① The two treatments of vinasse biomass ash + chemical fertilizer and lime + chemical fertilizer significantly increased soil pH (P < 0.05) to 3.39 and 2.25 units higher than that in the control, respectively. Compared with that in the control treatment, the content of available Cd in soil under vinasse biomass ash + chemical fertilizer and lime + chemical fertilizer treatment decreased by 28.91 % and 20.90 %, respectively. ② The contents of Cd in leaves, shells, and seeds of Zanthoxylum were decreased by 31.33 %, 30.24 %, and 34.01 %, respectively. The Cd enrichment ability of different parts of Zanthoxylum was different, with the specific performances being leaves > branches > seeds > shells. Compared with that of the control, the enrichment coefficient of each part of Zanthoxylum treated with vinasse biomass ash + chemical fertilizer decreased significantly(P < 0.05)by 27.54 %-40.0 %. ③ The changes in catalase and urease activities in soil treated with amendments were similar. Compared with those in the control group, the above two enzyme activities were significantly increased by 191.26 % and 199.50 %, respectively, whereas the acid phosphatase activities were decreased by 16.45 %. Correlation analysis showed that soil available Cd content was significantly negatively correlated with soil pH value(P < 0.01), S-CAT and S-UE enzyme activities were significantly positively correlated with soil pH(P < 0.01), and the soil available Cd content was significantly negatively correlated (P < 0.01); the S-ACP enzyme showed the complete opposite trends. The application of lime and vinasse biomass ash to acidic purple soil had the most significant effect on neutralizing soil acidity. It was an effective measure to improve acidic purple soil and prevent heavy metal pollution by reducing the effective Cd content in soil and improving the soil environment while inhibiting the absorption and transfer of Cd in various parts of Zanthoxylum.

Silicon and Seaweed Extract Injection into Olive Tree (Olea europaea L.) Trunks Results in the Tree’s Drought Stress Resistance

ABSTRACT Trunk injection applies more nutrients in the correct volume and composition, delivering ideal results with fewer fertilizer treatments under drought-stress conditions. We used a completely randomized block design to examine the effects of trunk injection therapy on the development of drought stress tolerance in olive trees in arid climates. Treatments included seaweed extract (SWE), potassium amino acid chelate (PAC), calcium silicate (CS), and potassium silicate (PS) at five different concentrations: 0.25, 0.5, 1, 2, and 4% for each treatment. Each treatment consisted of four replications – four dry replications for each treatment and four irrigation controls – each receiving weekly irrigation. The findings showed that, in comparison to drought control, trunk injection of PAC at a concentration of 2% enhances the relative water content of leaf and proline and decreases electrolyte leakage (EL) and malondialdehyde (MDA) content. Chlorophyll a, carotenoids, and proline content are all significantly higher in the trunk after CS injection at a 4% concentration than in the control treatment. MDA and EL were also reduced. Injecting PS leads to an increase in proline and photosynthetic pigments. The SWE treatment improved the proline, relative leaf water content, and photosynthetic pigments. To sum up, trunk injection treatments under drought conditions improved plant photosynthetic pigments, increased proline content, prevented relative water content reduction, and prevented an increase in MDA and EL. In conclusion, among the several treatments, the use of 2% PS was the most effective at reducing the adverse effects of drought stress on olive plants.

Transformation and Sequestration of Total Organic Carbon in Black Soil under Different Fertilization Regimes with Straw Carbon Inputs

In the context of the carbon peak and carbon-neutral era, it is crucial to effectively utilize maize straw as a resource for achieving carbon (C) sequestration and emission reduction in rural agriculture. Maize straw carbon undergoes two processes after being added to the soil: mineralization (decomposition) and humification (synthesis) by soil animals and microorganisms. These processes contribute to the reintegration of carbon into the agroecosystem’s carbon cycle. However, understanding of the transformation and stabilization of straw carbon, as well as the differences in C fixation capacity in soils with various fertilization treatments in black soils, remains limited. This study aims to quantify the relationship between straw carbon input and organic carbon sequestration in various fertilization treatments of black soil. Based on a long-term positional fertilization trial (45 years) in black soil, 13C-labeled maize straw (1.5 g in 120 g of dry soil) was applied and combined with an in situ incubation method using carborundum tubes. Throughout the 360-day trial, we observed the influence of fertilization on soil total organic C levels, organic carbon δ13C values, maize straw addition rate, and straw C fixation capacity. The decomposition of straw was most prominent during the initial 60 days of the incubation period, followed by a gradual decrease in the rate of decomposition. Compared with day 0, the SOC δ13C value and straw C residue rate were highest in the no-fertilization treatment (CK) after 360 days of incubation. The amount of organic carbon transformed and fixed in the soil was significantly higher in the organic fertilizer treatment (M) compared to other treatments, highlighting the stronger decomposition, transformation, and carbon fixation capacity of straw carbon in the M treatment. Moreover, the highest carbon storage of 43.23 Mg·ha−1 was observed in the M fertilization treatment after 360 days, which was significantly different from other treatments (p < 0.05). The study demonstrates that soil with low fertility exhibits increased sequestration potential for straw carbon. Additionally, organic fertilizer input would increase soil organic carbon storage and facilitate straw carbon conversion.

Divergent accumulation of microbial necromass and plant lignin phenol induced by adding maize straw to fertilized soils

Plant carbon (C) inputs and their subsequent microbial transformation affect the build-up process of soil organic C (SOC) pool. Nevertheless, there are knowledge gaps on how crop straw addition modifies SOC composition at molecular-level, especially in soils with different fertilization practices. Here, long-term fertilized Mollisols (unfertilized control, NF; inorganic fertilization, IF; inorganic fertilization plus manure, IFM) were incubated with or without maize straw addition in a 900-day field mesocosm experiment. The microbial necromass and plant lignin components contents were synchronously quantified based on amino sugar and lignin phenol biomarkers, respectively. And changes in SOC chemical composition were examined using solid-state 13C nuclear magnetic resonance spectroscopy. Relative to the NF treatment, long-term fertilization increased amino sugar and lignin phenol contents, and manure application enhanced the accumulation of plant lignin components more than that of microbial necromass. Compared with the NF treatment, the IF treatment decreased the relative proportion of alkyl C and SOC content, suggesting that changes in microbial necromass and lignin phenol were not always consistent with changes in SOC. For the NF and IF treatments, maize straw incorporation increased both amino sugar content and its contribution to SOC, indicating that microbial anabolism was important for SOC accumulation after adding maize straw in C-poor soils. For the IFM treatment, maize straw addition decreased lignin phenol content (27 %) and its contribution to SOC but increased the contribution of amino sugar to SOC, reflecting an enlarged contribution of microbial necromass to soil C pool formation under manure application after maize straw addition. The contribution of lignin phenol to SOC was decreased from days 360–900, whereas fungal necromass C content and the contribution of amino sugar to SOC were increased from days 360–900 under the IFM soil with maize straw addition, indicating that plant lignin components might be converted into fungal biomass and its necromass with increasing maize straw decomposition under manure application. Overall, we concluded that maize straw addition favored microbial immobilization in all fertilization treatments.