Divergent accumulation of microbial necromass and plant lignin phenol induced by adding maize straw to fertilized soils

Abstract

Plant carbon (C) inputs and their subsequent microbial transformation affect the build-up process of soil organic C (SOC) pool. Nevertheless, there are knowledge gaps on how crop straw addition modifies SOC composition at molecular-level, especially in soils with different fertilization practices. Here, long-term fertilized Mollisols (unfertilized control, NF; inorganic fertilization, IF; inorganic fertilization plus manure, IFM) were incubated with or without maize straw addition in a 900-day field mesocosm experiment. The microbial necromass and plant lignin components contents were synchronously quantified based on amino sugar and lignin phenol biomarkers, respectively. And changes in SOC chemical composition were examined using solid-state 13C nuclear magnetic resonance spectroscopy. Relative to the NF treatment, long-term fertilization increased amino sugar and lignin phenol contents, and manure application enhanced the accumulation of plant lignin components more than that of microbial necromass. Compared with the NF treatment, the IF treatment decreased the relative proportion of alkyl C and SOC content, suggesting that changes in microbial necromass and lignin phenol were not always consistent with changes in SOC. For the NF and IF treatments, maize straw incorporation increased both amino sugar content and its contribution to SOC, indicating that microbial anabolism was important for SOC accumulation after adding maize straw in C-poor soils. For the IFM treatment, maize straw addition decreased lignin phenol content (27 %) and its contribution to SOC but increased the contribution of amino sugar to SOC, reflecting an enlarged contribution of microbial necromass to soil C pool formation under manure application after maize straw addition. The contribution of lignin phenol to SOC was decreased from days 360–900, whereas fungal necromass C content and the contribution of amino sugar to SOC were increased from days 360–900 under the IFM soil with maize straw addition, indicating that plant lignin components might be converted into fungal biomass and its necromass with increasing maize straw decomposition under manure application. Overall, we concluded that maize straw addition favored microbial immobilization in all fertilization treatments.