A field experiment was conducted during rabi season of (2020-21) on wheat at Research Farm of Kulbhaskar Ashram Post Graduate College, Prayagraj (UP), to find out the Response of Chemical Fertilizers and INM on Productivity of Wheat (Triticum aestivum L.), and Properties of Soil. The experiment laid out in Randomized Block Design (RBD) with eleven treatments and three replications. The results revealed that the highest plant height was recorded in treatment T11 (75% RDF +5 t ha1 FYM +20 kg ha1 ZnSO4 +Azotobacter and PSB), which was significantly superior over treatment T3, T4 and T5. The maximum yield attributing characters (number of spikes per m2, spike length, number of grains per spike and test weight), and yield (grain and straw), were increased with treatment receiving 75% RDF +5 t ha1 FYM +20 kg ha1 ZnSO4 +Azotobacter and PSB (T11). The maximum enhancement in grain and straw yield of wheat was recorded in treatment T11 (75% RDF +5 t ha1 FYM +20 kg ha1 ZnSO4 +Azotobacter and PSB), which was significantly superior over T2, T3, T4, T5, T8 and T9. The maximum protein content (13.18%), in grain of wheat was found in treatment T11 (75% RDF +5 t ha-1 FYM +20 kg ha1 ZnSO4 +Azotobacter and PSB), The application of FYM with Chemical Fertilizers and Bio-fertilizers (Azotobacter and PSB) improved in soil pH and EC and also increased the Soil Organic Carbon and Available N, P & K in soil after the harvest of crop. The treatment (T11). was found best in respect of soil properties.