Introduction: India is facing a demographic nightmare in terms of gender imbalance, The worrying fact is the child sex ratio in Nashik district is 890 which is much lower than the national average of 919. This rate of decline is alarming. Though government has implemented PC & PNDT Act to prevent pre-natal sex determination, it seems not to be reflected in practice. Aims & Objectives: To assess effect of awareness programme on the Knowledge, Attitude and Practice of Gender Preference & Pre-natal sex determination in population of Nashik district. Materials and methods: A cross-sectional study was carried out in the antenatal ward of a tertiary care hospital, Nashik district. A total of 100 women were included and a pre-designed and pre-tested questionnaire was used. They were asked regarding the knowledge and attitude towards the PNDT Act. There was Awareness session on PC & PNDT act and Sex Ratio, post which the questionnaire was given again. Data was analyzed by using Statistical Package of Social Sciences (SPSS) v.22.0. Results: In the Present Study, knowledge regarding PC&PNDT act was known by 88% participants. Gender preference of a particular sex in families was 45% which came down to 12% during the post awareness. Their desire to know gender of foetus came down to 4% from 15% after the awareness programme. Conclusion: The existence of son preference is at an alarming high rate in our society and is the most important – the root cause of imbalanced sex ratio. Thus apart from the legislative measures, people also needs to be educated about the ethical issues related to female foeticide
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