Choosy Females Research Articles

Mitochondrial function and sexual selection: can physiology resolve the 'lek paradox'?

Across many taxa, males use elaborate ornaments or complex displays to attract potential mates. Such sexually selected traits are thought to signal important aspects of male 'quality'. Female mating preferences based on sexual traits are thought to have evolved because choosy females gain direct benefits that enhance their lifetime reproductive success (e.g. greater access to food) and/or indirect benefits because high-quality males contribute genes that increase offspring fitness. However, it is difficult to explain the persistence of female preferences when males only provide genetic benefits, because female preferences should erode the heritable genetic variation in fitness that sexually selected traits signal. This 'paradox of the lek' has puzzled evolutionary biologists for decades, and inspired many hypotheses to explain how heritable variation in sexually selected traits is maintained. Here, we discuss how factors that affect mitochondrial function can maintain variation in sexually selected traits despite strong female preferences. We discuss how mitochondrial function can influence the expression of sexually selected traits, and we describe empirical studies that link the expression of sexually selected traits to mitochondrial function. We explain how mothers can affect mitochondrial function in their offspring by (a) influencing their developmental environment through maternal effects and (b) choosing a mate to increase the compatibility of mitochondrial and nuclear genes (i.e. the 'mitonuclear compatibility model of sexual selection'). Finally, we discuss how incorporating mitochondrial function into models of sexual selection might help to resolve the paradox of the lek, and we suggest avenues for future research.

Alcohol reduces choosiness and relaxes mate preferences in female Drosophila simulans

Abstract Alcohol can alter sexual behaviour. However, our understanding of alcohol’s effects on sexual interactions is limited because males are usually the focus of nonhuman studies. Alcohol could in theory alter female mate-choice and, in turn, affect sexual selection, a major mechanism of evolution. Here we tested whether alcohol exposure affected female mate-preference, choosiness, and copulation duration in the fly Drosophila simulans, while simultaneously testing for genetic variation in these effects. We found that alcohol exposure did not affect copulation duration, but did weaken mate-preference, as females copulated with a broader range of males after exposure, and it tended to reduce female choosiness as females mated more quickly. Although female genotype significantly affected copulation duration and choosiness, the effects of alcohol appeared to be reasonably consistent across genotypes, but this depended on the analysis employed. Nonetheless, alcohol reduced female choosiness and weakened mate-preference, and may therefore reduce the strength of sexual selection. Testing whether these changes impact female fitness is now required.

From Father to Son: Passing Down the Secrets of Paternal Success.

Males and females typically share the same genome. Intra-locus sexual conflict is when the needs of each sex exert unique selection pressures on the shared genome. We are interested in investigating the effects of intra-locus sexual conflict by removing one of the two sexes from the conflict. Male limited (ML) selection allows males to directly pass genes to their sons without female selection playing a role. This is facilitated through clone generator females with translocated and marked autosomes and conjoint X chromosomes that induce reversed inheritance of sex chromosomes. ML males should be able to accumulate changes beneficial to male reproductive fitness much more quickly than matched control (MC) males bound by bilateral gene expression. We have conducted 75 generations of this male-limited protocol on Drosophila melanogaster flies and have demonstrated that ML flies show higher fitness within ML conditions.
 In nature, male flies compete with each other to sire offspring, as a result of genetic differences, some males become more successful. We assayed the post copulatory success of our target males by introducing target males to control females after the females were mated with recessively marked Control males. This design allows for adaptations resulting from selection to be investigated, adaptations can either result from the choosiness of females or the physical traits of males like the shape, number, and chemicals contained in sperm.
 My assays found that experimental males tended to be more successful in sperm offence when compared to control males within the ML protocol. Males with double dose ML chromosomes performed significantly worse than Controls, suggesting that adaptation to overcome genetic artifacts from the ML protocol (mutant backgrounds and haploid selection) was predominant to resolving intra-locus sexual conflict. There is also the possibly that a lack of genetic variation prevented adaptations from arising.

Timing matters: female receptivity and mate choice in the zebrafish (Danio rerio)

Female choice has been documented in many animal taxa, and how we test it has been refined through years of studies on the topic. However, when designing mate choice experiments some variables, surprisingly, often remain overlooked, including receptivity and reproductive stage. Here, we aimed to assess whether the female reproductive stage influences strength and direction of mate choice in the zebrafish, Danio rerio. Females were offered a choice between two males differing in body size. We found that female choice in our experimental setup was significantly repeatable and that females preferred larger males. Nonetheless, the level of choosiness of females was affected by the time since the last spawning. Females spent more time choosing when tested 7 and 10 days after spawning rather than 4 days, indicating a higher receptivity to males from one week after the last spawning. Moreover, females preferred larger males only when tested 7 and 10 days after spawning. Our results suggest that female mate choice should take female receptivity into account, by standardizing time since the last spawning across females. More broadly, this suggests that 7–10 days since the previous spawning is the ideal time interval for zebrafish female receptivity to peak, with implications for facilities and researchers to increase egg production in natural spawning events and manual egg collection.Significance statementThe role of pre-copulatory female mate preference has long been recognized in sexual selection. Nonetheless, female receptivity often remains overlooked in mate choice experiments especially in external fertilizing species. In the present study, we investigated if the female reproductive stage affects the strength and direction of female mate choice in an external fertilizing fish, the zebrafish, Danio rerio. We found that, when tested 7 and 10 days after spawning rather than 4 days, females spent more time choosing, demonstrating an increased receptivity to males from 1 week following the last spawning. Furthermore, only at 7 and 10 days after spawning females exibith a clear preference for the bigger males. Our study highlights the importance of considering the female receptivity in future studies assessing mate choices in this and other externally fertilizing species, and also for zebrafish facilities to increase egg production in natural spawning events and manual egg collection.

Evolution of choosiness dictates whether search costs of mate choice enhance speciation by sexual selection

The role of sexual selection in speciation is implicated in both empirical case studies and larger comparative works. However, sexual selection faces two major problems in driving speciation. First, because females with novel preferences search for their initially rare males, search costs are expected to curtail initial sexual divergence. Second, if these populations come back into sympatry, sexual divergence may be erased due to hybridization. A major goal is to understand which conditions increase the likelihood of overcoming these problems. Here we generated a diploid population genetic model of how female search costs and evolution of female 'choosiness' (i.e. preference strength) interact to drive speciation in allopatry and secondary contact. We studied the model using numerical simulations in the context of two different male traits, ecologically 'arbitrary' versus 'magic' traits. First, in allopatry, without female search costs only minor and fluctuating sexual isolation evolved. In contrast, with female search costs, sexual isolation was highly curtailed with arbitrary male traits but was greatly facilitated with magic traits. However, because search costs selected for reduced choosiness, sexual isolation with magic traits was eventually eroded, the rate determined by the genetic architecture of choosiness. These factors also played a key role in secondary contact; with evolvable choosiness and female search costs, pure sexual selection models collapsed upon secondary contact. However, when we added selection against hybrids (i.e. reinforcement) to this model, we found that speciation could be maintained under a wide range of conditions with arbitrary male traits, but not with magic male traits. This surprisingly suggests that arbitrary male traits are in some cases more likely to aid speciation than magic male traits. We discuss these findings and relate them to empirical literature on female choosiness within species and in hybrids.

Parasite-Mediated Mate Preferences in a Cooperatively Breeding Rodent

Females of many species discriminate among males when choosing a mate and this can bear indirect and direct benefits including the avoidance of parasite transmission from infested males. In rodents, this may be mediated by androgen hormones that affect the expression of urinary odors. Female choosiness may also vary with a female’s infestation status, with infested females being less choosy. In the current study we tested the preference of cooperatively breeding highveld mole-rat (Cryptomys hottentotus hottentotus) females for male urinary odors from healthy males and those naturally infested with a cestode (Mathevotaenia sp.). Thirty females (15 healthy, 15 infested) were allowed to explore a Y-maze with urine samples from healthy and infested males and the frequency of entering choice arms and chambers as well as the duration spend with each odor sample was recorded. Infestation status did neither affect male body mass, urinary testosterone, nor cortisol levels or the body condition of females. Although overall female activity was not affected by infestation status, infested females entered choice arms and chambers significantly less frequently than healthy females. Surprisingly, healthy females preferred odors from infested males while the opposite was true for infested females, independent of male hormone levels. As the study species lives in groups that tend to share the same infestation status, we suggest that highveld mole-rat females may exhibit a preference for unfamiliar odors, possibly as an indicator of genetic diversity, rather than discriminate between infestation status of males. Similar mechanisms may also play a role in other social species.

Hybridization despite elaborate courtship behavior and female choice in Neotropical tree frogs.

The mechanisms of hybridization can be elucidated by analyzing genotypes as well as phenotypes that could act as premating barriers, as the reproductive interactions among heterospecifics can alter the evolutionary history of species. In frogs, hybrids typically occur among species that reproduce explosively (in dense aggregations) with few opportunities for mate selection but are rare in species with elaborate courtship behaviors that may prevent erroneous mating. Using 21 microsatellite markers, we examined hybridization in the prolonged-breeding tree frogs Bokermannohyla ibitiguara and B. sazimai sampled within a contact zone in the Brazilian savanna (72 tadpoles; 74 adults). We also compared acoustic and morphological data. We confirmed both parental species genetically; STRUCTURE results confirmed 14 hybrids, 11 of which were second-generation according to NEWHYBRIDS, all with intermediate values of genetic dissimilarities compared to the parentals. Morphological and acoustic analyses revealed that hybrids showed variable but not necessarily intermediate phenotypes. Moreover, 2 hybrids exhibited call types different from parentals. The reproduction of B. ibitiguara involves territorial and aggressive males, elaborate courtships with acoustic and tactile stimuli, choosy females, and opportunistic strategies. Our study uncovers a rare case of viable hybridization among closely related frogs with such a combination of complex courtship behaviors and mate choice. We discuss the likely directionality and mechanisms behind this phenomenon, and highlight the importance of investigating hybridization even in species that show elaborate reproduction and female choice to advance our understanding of animal diversification.

The evolution of age-specific choosiness and reproductive isolation in a model with overlapping generations.

The strength of mate choice (choosiness) often varies with age, but theory to understand this variation is scarce. Additionally, theory has investigated the evolution of choosiness in speciation scenarios but has ignored that most organisms have overlapping generations. We investigate whether speciation can result in variation of choosiness with age, and whether such variation can in turn affect speciation. We develop a population-genetic model of the evolution of choosiness in organisms with overlapping generations in the context of secondary contact between two divergent populations. We assume that females choose males that match their phenotype, such that choosiness evolves by sexual selection. We demonstrate that speciation can result in the evolution of age-specific choosiness when the mating trait is under divergent ecological selection and age is not used as a mating cue. The cause of this result is that allele frequencies differ between choosy females and males. However, we find that the evolution of age-specific choosiness does not affect the overall level of reproductive isolation compared to a case without age-structure, supporting previous speciation theory. Overall, our results connect life history and speciation theory, and the mechanisms that we highlight have implications for the understanding of the role of sex-specific selection in the evolution ofchoosiness.

Maturational changes in song sparrow song

Age‐related changes in the production of sexually selected assessment signals have been identified across a diverse range of taxa, and in some cases, these changes have been shown to affect receiver response to those signals. One important type of change occurs even after a signaler reaches breeding age, a phenomenon known as delayed maturation. Delayed maturation has been observed in the songs of several bird species, with potential fitness consequences for males as a byproduct of female choosiness or male competition. Here, we analyzed songs recorded across the first three years of life in a cohort of hand‐reared song sparrows Melospiza melodia to detect early‐life age‐related changes in song. We focused on three measures of song complexity, including within song type variation, the average number of notes and the number of unique note types for the most common variant of each song type, and five measures of song production patterns, including singing rate, time interval between songs within a bout of the same song type, time interval between bouts (i.e. when the song type changes), within‐song stereotypy and between‐song consistency. All measures of song complexity and most measures of song production patterns (excluding within‐song stereotypy) changed significantly within individuals as birds aged from one to two years as well as from one to three years (excluding within‐bout time interval), whereas no significant changes occurred from two to three years of age. Based on these features, a linear discriminant model could distinguish between the song of young (age 1) and older (age 2 and 3) adult males, providing support that song could serve as an indicator of age in this species. We discuss potential implications of these results for mate choice and male–male competition in song sparrows.

Fitness costs of female choosiness are low in a socially monogamous songbird.

Female mate choice is thought to be responsible for the evolution of many extravagant male ornaments and displays, but the costs of being too selective may hinder the evolution of choosiness. Selection against choosiness may be particularly strong in socially monogamous mating systems, because females may end up without a partner and forego reproduction, especially when many females prefer the same few partners (frequency-dependent selection). Here, we quantify the fitness costs of having mating preferences that are difficult to satisfy, by manipulating the availability of preferred males. We capitalize on the recent discovery that female zebra finches (Taeniopygia guttata) prefer males of familiar song dialect. We measured female fitness in captive breeding colonies in which one-third of females were given ample opportunity to choose a mate of their preferred dialect (two-thirds of all males; "relaxed competition"), while two-thirds of the females had to compete over a limited pool of mates they preferred (one-third of all males; "high competition"). As expected, social pairings were strongly assortative with regard to song dialect. In the high-competition group, 26% of the females remained unpaired, yet they still obtained relatively high fitness by using brood parasitism as an alternative reproductive tactic. Another 31% of high-competition females paired disassortatively for song dialect. These females showed increased levels of extra-pair paternity, mostly with same-dialect males as sires, suggesting that preferences were not abolished after social pairing. However, females that paired disassortatively for song dialect did not have lower reproductive success. Overall, females in the high-competition group reached equal fitness to those that experienced relaxed competition. Our study suggests that alternative reproductive tactics such as egg dumping can help overcome the frequency-dependent costs of being selective in a monogamous mating system, thereby facilitating the evolution of female choosiness.

Juvenile Experience with Male Cues Triggers Cryptic Choice Mechanisms in Adult Female Redback Spiders.

Simple SummaryFemales of many species vary in their receptivity to male mating attempts. When many males are present in the habitat, the theory predicts that females should be choosy and discriminate among potential mates. When few males are available, females should mate readily with the first male who courts, and thus avoid the risk of remaining unmated. We predicted that cues perceived as juveniles that indicate male availability would affect the mating behaviour of adult females. In our first experiment, juvenile females were exposed to airborne chemicals produced by males at high or low densities. In our second experiment, we mimicked a natural situation where males or other juveniles live on the webs of females shortly before they become sexually mature, and compared this to females developing in isolation. As was consistent with our predictions, we found that the adult females changed their behaviour after exposure to cues of high male availability during development. When the females perceived many males nearby (high density airborne cues or living with males) they more often interrupted copulation, or cannibalized the males before the mating was complete as adults. In comparison, when the cues indicated low male availability, the adult females were more likely to allow the males to complete mating, and cannibalism was less common.Female choice may be linked to population density if the expected encounter rates with potential mates affects choosiness (the energy and risk engaged to express mate preferences). Choosiness should covary with male availability, which could be assessed using the social cues available during development. We tested whether the exposure of juvenile females to cues of male density affected the mechanisms of choosiness of adult Latrodectus hasselti spiders in two experiments simulating natural contexts. The juvenile females were exposed to (1) volatile chemicals from two densities of adult males (airborne cues), and (2) tactile, vibrational and chemical cues from adult males or other females (cohabitation cues). As adults, the females mated readily, regardless of the treatment, but there was strong evidence for post-copulatory mechanisms of choosiness in females exposed to cues of high male availability. These included abbreviated matings (in both experiments), cannibalism of the males before the mating was complete (cohabitation), and, remarkably, a reduction in the successful placement of internal sperm plugs (cohabitation). These shifts decrease the likelihood that the first mate would monopolize paternity if the female chose to mate again. We conclude that female choosiness may impose a strong selection on males despite the high mating rates, and these effects can hinge on the cues of male availability detected by juveniles.

Mating increases Drosophila melanogaster females' choosiness by reducing olfactory sensitivity to a male pheromone.

Females that are highly selective when choosing a mate run the risk of remaining unmated or delaying commencing reproduction. Therefore, low female choosiness would be beneficial when males are rare but it would be maladaptive if males become more frequent. How can females resolve this issue? Polyandry would allow mating-status-dependent choosiness, with virgin females selecting their first mate with little selectivity and becoming choosier thereafter. This plasticity in choosiness would ensure timely acquisition of sperm and enable females to increase offspring quality during later mating. Here, we show that Drosophila melanogaster females display such mating-status-dependent choosiness by becoming more selective once mated and identify the underlying neurohormonal mechanism. Mating releases juvenile hormone, which desensitizes Or47b olfactory neurons to a pheromone produced by males, resulting in increased preference for pheromone-rich males. Besides providing a mechanism to a long-standing evolutionary prediction, these data suggest that intersexual selection in D. melanogaster, and possibly in all polyandrous, sperm-storing species, is mainly the domain of mated females since virgin females are less selective. Juvenile hormone influences behaviour by changing cue responsiveness across insects; the neurohormonal modulation of olfactory neurons uncovered in D. melanogaster provides an explicit mechanism for how this hormone modulates behavioural plasticity.

Does the choosiness of female crickets change as they age?

ABSTRACTFor crickets, which approach singing males by phonotaxis, the female choosiness hypothesis postulates that young females should be more selective of male calling song patterns than older individuals. However, there is no information about the behavioural preferences of females over their complete adulthood. We analysed phonotaxis in female Gryllus bimaculatus throughout their entire adult lifetime and measured the impact of sound amplitude, carrier frequency and the temporal pattern of test songs on their auditory response. Females of all ages demonstrated their best responses to male calling songs with a pulse period of 34–42 ms, a carrier frequency of 4.5 kHz and a sound pressure level of 75 dB. The response profile to somewhat less optimal song types did vary with age, but not in a manner consistent with a simple loosening of selectiveness in older females. Age, however, had an effect on the overall strength of phonotaxis, as very old females showed an overall diminishing response to all song types. Our data suggest that although there are minor changes in the relative preferences of crickets to individual song elements as they age, the breadth of song patterns to which they will perform phonotaxis remains similar across age groups.

Female mating status affects male mating tactic expression in the wolf spider Rabidosa punctulata.

Males and females have conflicting interests on the frequency and outcomes of mating interactions. Males maximize their fitness by mating with as many females as possible, whereas choosy females often reduce receptivity following copulation. Alternative male mating tactics can be adaptive in their expression to a variety of mating contexts, including interactions with a relatively unreceptive mated female. Male Rabidosa punctulata wolf spiders can adopt distinctive mating tactics when interacting with a female, a complex courtship display, and/or a more coercive direct mount tactic that often involves grappling with females for copulation. In this study, we set up female mating treatments with initial trials and then paired mated and unmated females with males to observe both female remating frequencies and the male mating tactics used during the interactions. Males adopted different mating tactics depending on the mating status of the female they were paired with. Males were more likely to adopt a direct mount tactic with already-mated females and courtship with unmated females. Already-mated females were considerably less receptive to males during experimental trials, although they did remate 34% of the time, the majority of which were with males using a direct mount tactic. Whereas males adjusting to these contextual cues were able to gain more copulations, the observation of multiple mating in female R. punctulata introduces the potential for sperm competition. We discuss this sexual conflict in terms of the fitness consequences of these mating outcomes for both males and females.

Choosy females drive isolation

Speciation Rapid radiations of recently diverged species represent an excellent opportunity for exploring drivers of speciation. The capuchino seedeaters, a group of South American birds, include a number of species that, in the field, are often discernable only through male plumage and song. Turbek et al. used genomes and behavioral experiments to identify potential isolating factors in two members of this group and found that, though entirely sympatric, females mated only with conspecific males and that only a few genes differed between the species (see the Perspective by Jarvis). Thus, a small reshuffling of genes and reinforcement through mate choice has driven divergence in these overlapping and very similar species. Science , this issue p. [eabc0256][1]; see also p. [1312][2] [1]: /lookup/doi/10.1126/science.abc0256 [2]: /lookup/doi/10.1126/science.abg5454

Moderately elevated glucocorticoids increase mate choosiness but do not affect sexual proceptivity or preferences in female gray treefrogs

Glucocorticoids (GCs) are rarely studied in the context of female mate choice, despite the expression of receptors for these products in sexual, sensory and decision-making brain areas. Here we investigated the effects of GC concentrations on three aspects of female sexual behavior in breeding Cope's gray treefrogs (Hyla chrysoscelis): proceptivity—a measure of sexual motivation, intraspecific mate preferences, and mate choosiness. To our knowledge this is the first experimental study on the endocrine basis of mate choosiness. We predicted that mate choosiness—forfeiting an initial mate preference to pursue a suddenly more attractive mate—would be particularly impacted by elevated GCs with moderate GC levels associated with greater choosiness. We found support for this predicted inverted-U relationship. Females in the control group (no injection) showed no change in choosiness across timepoints. In contrast, females in the vehicle, Low (20 ng g−1) and High (180 ng g−1) corticosterone groups exhibited a nominal decline in choosiness after injection, suggesting that the experience of injection has little or perhaps slightly suppressive effects on female choosiness. Females in the moderate dose group (60 ng g−1), however, exhibited a significant increase (>100%) in choosiness. Further, we found no effect of elevated GCs on sexual proceptivity or the species-typical preference for longer calls. These findings may reflect a buffering of primary sensory areas in the brain against elevated GCs. The recruitment of other cognitive processes during active decision-making, however, may facilitate GC modulation of mate choosiness, thereby promoting tactical plasticity at this critical life history juncture.

The size of signal detection and emission organs in a synchronous firefly: sexual dimorphism, allometry and assortative mating.

The size of the organs responsible for emitting and detecting sexual communication signals is a likely target for selection. Communication via bioluminescent signals in synchronous fireflies is a promising model to test hypotheses regarding differences between males and females in the effect of the size of signal emission and detection organs on fitness components. Synchronous firefly species congregate in large numbers during the mating season, displaying bioluminescent signals aimed at potential mates during relatively short nightly periods. Operational sex ratios are male-biased and, thus, the so-called typical sex roles (indiscriminate males and choosy females) are expected to evolve. We studied the synchronous firefly Photinus palaciosi, a species that during the mating season congregates in forests of central Mexico offering a magnificent natural show that attracts numerous tourists. P. palaciosi females have reduced wings (brachyptery) and cannot fly. Our field study tested the hypothesis that the male-biased operational sex ratio and the short daily mating period result in strong male-male competition that selects for males with larger lanterns and larger eyes, and against male mate choice, whereas female-female mate competition is absent and, thus, no selection on lantern or eye size is expected. Even though lantern, eye or body size do not predict the probability of being found in copula for either sex, sexual dimorphism in these features, along with allometric slopes of lantern size and assortative mating in terms of relative lantern size, support not only the hypothesis of intense sexual selection among males, but the possibility of subtle mechanisms of sexual selection among females. Trade-offs between investment in signaling (lanterns) versus detection (eyes) structures, or with pressures different from sexual selection such as those imposed by predators, are also likely to be important in shaping the evolution of sexual signaling in these fireflies.

Males mate indiscriminately in the tropical jumping spider Hasarius adansoni (Audouin, 1826)

AbstractTraditionally, sexual selection has been seen as a process in which choosy females select non‐choosy males. Recent studies, however, have challenged this view by showing that males can also be choosy in many species. We assessed the sexual preferences in males of the tropical jumping spider Hasarius adansoni (Audouin, 1826). We measured mating effort in males and determined how female quality influences offspring quality, quantity, and survival. We also estimated total sperm load and how much sperm was invested in a mating with a particular female. We found no evidence of male mating effort in terms of mating frequency nor sperm investment. Similarly, there was no relationship between female quality (i.e., size and condition) and offspring quality (i.e., survival and feeding performance) or quantity. We found strong evidence that the sperm invested in a particular female is a function of the amount of sperm the male had available for usage at that particular mating. The fact that males probably find females sequentially, along with the lack of relationship between female quality and offspring quality/quantity, likely explains the lack of differences in mating effort by males.

New finding of melanic three-spined sticklebacks Gasterosteus aculeatus in the Scottish Hebrides

Colour traits can be elaborated through sexual selection and have potential to drive reproductive isolation. Male three-spined sticklebacks (Gasterosteus aculeatus) express striking visual signals to attract choosy females during courtship, typically expressed as red carotenoid-based pigmentation on their throat and jaw during the breeding season, along with blue eyes and blue/green flanks. The extent and intensity of red colouration in males have been linked to fitness benefits to females, including body condition, parasite resistance, parental ability and nest defence. In some populations in the Pacific Northwest of North America, male three-spined sticklebacks express melanic nuptial colouration. In these populations, male possess black throats instead of red, and have dark or black bodies. Melanic males are associated with waterbodies that are red-shifted due to the presence of tannins, where the ambient light environment is dominated by long wavelengths. Here we report the first discovery outside North America of melanic populations of three-spined sticklebacks on the island of North Uist in the Scottish Hebrides, on the northwest Atlantic coast of Europe. These populations are associated with a hotspot of stickleback morphological diversity and occur in association with red-shifted waterbodies.

Black widows as plastic wallflowers: female choosiness increases with indicators of high mate availability in a natural population

Female choice is an important driver of sexual selection, but can be costly, particularly when choosy females risk remaining unmated or experience delays to reproduction. Thus, females should reduce choosiness when mate encounter rates are low. We asked whether choosiness is affected by social context, which may provide reliable information about the local availability of mates. This has been demonstrated in the lab, but rarely under natural conditions. We studied western black widow spiders (Latrodectus hesperus) in the field, placing experimental final-instar immature females so they were either ‘isolated’ or ‘clustered’ near naturally occurring conspecifics (≥10 m or ≤1 m, respectively, from a microhabitat occupied by at least one other female). Upon maturity, females in both treatments were visited by similar numbers of males, but clustered females were visited by males earlier and in more rapid succession than isolated females, confirming that proximity to conspecifics reduces the risk of remaining unmated. As predicted, isolated females were less choosy in staged mating trials, neither rejecting males nor engaging in pre-copulatory cannibalism, in contrast to clustered females. These results demonstrate that exposure of females to natural variation in demography in the field can alter choosiness of adults. Thus, female behaviour in response to cues of local population density can affect the intensity of sexual selection on males in the wild.