Animal Feed Research Articles

Effects of some locally isolated Lactobacillus species on general growth, muscular mass and bone development in mice

Probiotics are dietary supplements which contain live microorganisms, mostly different bacterial species that inhabit gut of host and ultimately benefit that host. The current investigation was done to explore the growth-promoting effects of four locally isolated probiotics strains L. paracasei, L. paracaseii, L. delbrueckii and L. rhamnosus. In this regard, 108 colony-forming units (CFU) were administered to the mice of experimental groups for 30 days. Upon administering probiotics, it was observed that the Lactobacilli species had a positive impact on body weight. The group that received L. rhamnosus exhibited the most significant increase, with a 76.53 % improvement over the control group (P < 0.05). Positive effects on somatic indices of different organs including spleen, thymus, kidneys, heart, liver, and lungs were also observed. In addition to all of these, the increases in the number of extensor digitorum longus (EDL) muscle fibers and their cross-sectional area (CSA) up to 40.70 % and 28.94 % respectively in L. rhamnosus group as compared with the control group. In the L. rhamnosus group, there was a 40.70 % increase in the number of EDL muscle fibers and a 28.94 % increase in their cross-sectional area (CSA) of muscle fiber as compared to the control group. On provision of Lacticaseibacillus rhamnosus, 50.81 % higher femur bone density was noted which endorse the overall health-improving abilities of this probiotic. The application of Lacticaseibacillus rhamnosus in animal feed is recommended.

Intercropping impacts growth in the forage cactus, but complementarity affords greater productivity, competitive ability, biological efficiency and economic return

CONTEXTSoil and climatic conditions limit forage production in semi-arid environments. Planting crops adapted to these elements, combined with management practices such as intercropping and irrigation, can ensure the sustainability of animal feed. OBJECTIVEThe aim of this study was to evaluate growth, forage productivity, soil water balance, water indices and the indices of biological efficiency, competitive ability, and economic return in a dense crop of forage cactus, grasses, and legumes under single and intercropping systems in a semi-arid environment. METHODSThe study was carried out from 2020 to 2022 in Serra Talhada, Pernambuco, Brazil. The plants used were the ‘Orelha de Elefante Mexicana’ (OEM) clone of the forage cactus, pigeon pea (‘crioula’), millet (‘Bulk-01’) and sorghum (‘SF15’). A randomised block design was adopted, with three repetitions, including single crops of the forage cactus, sorghum, millet and pigeon pea, as well as the cactus intercropped with pigeon pea, millet and sorghum. Biometrics and biomass were checked to determine morpho-physiological indices, phenology, cutting time, biological efficiency and competitive ability. The soil moisture was also monitored, and the soil water balance quantified. The water indices and economic return were then calculated. RESULTS AND CONCLUSIONSGrowth rates and phenology were not affected by intercropping, only by the cutting time. During the first season, the single sorghum and cactus-pigeon pea system showed a variation in soil water storage of 26 mm, while during the second season, the single cactus stored 66.74 mm. The actual evapotranspiration ranged from 1109 in the cactus-millet system to 1152 mm in the single cactus and millet (1st season), and from 1510 in the single cactus to 1570 mm in the sorghum (2nd season). The cactus-sorghum system was the most productive, with 460.53 Mg FM ha−1 and 43.55 Mg DM ha−1 in season one, while in season two, the single cactus obtained 237.64 Mg FM ha−1 and 26.71 Mg DM ha−1. Intercropping promotes more productive stability and feed diversity, as well as greater biological efficiency, competitive ability and economic return. SIGNIFICANCEUnderstanding the changes that exist in a intercropping system is important, especially in semi-arid regions, since these have distinct characteristics that impact the development of crops. Thus, this work contributes comprehensively to resilient practices for the semi-arid, seeking to ensure the sustainability of the intercropping systems and understand the interrelationship between species from the management adopted.

Sustainable solutions for water hyacinth invasion: Characteristics, impacts, control, and utilization

Water hyacinth (Eichhornia crassipes) is an invasive aquatic plant that has become a global issue due to its significant ecological and socio-economic impacts. This paper discusses the characteristics and impacts of water hyacinth invasion, emphasizing the urgency of effective management strategies. Various methods for harvesting and controlling water hyacinth, including physical, biological, and chemical approaches, are elucidated. Additionally, the paper highlights the potential utilization of water hyacinth for pollutant absorption and sequestration of heavy metals, organic contaminants, and excess nutrients. Furthermore, the opportunities to convert water hyacinth biomass into bioenergy sources such as biogas and bioethanol have been identified. The paper also presents the technical feasibility of using water hyacinth to produce biochar, high-quality bio-fertilizer, animal feed, and several other valuable products.

The ethnobotanical heritage of Lotkuh, a high-altitude tribal haven of Chitral, the Eastern Hindu Kush, Pakistan

BackgroundIn northwestern Pakistan, Lotkuh is a high-altitude terrain nestled within the eastern Hindu Kush region. Enclaved by towering peaks and harboring a unique culture, the region mirrors the geographical and cultural diversity of Pakistan. In this geographically isolated region, a treasure trove of ethnobotanical knowledge unfolds through generations of interaction between the inhabitants and indigenous plants, resulting in a profound understanding of the plant uses in nutritional, medicinal, cultural, and ritual contexts. Thus, the study seeks to gather, analyze, and document the indigenous knowledge of plant utilization of the distinct tribal culture.MethodsThrough semi-structured questionnaires, inventory interviews, and participatory workshops, data were collected by engaging a cohort of 120 local respondents. The collected data were then classified into nine distinct use categories, following which quantitative indices were calculated.ResultsThe research identified a total of 150 plant species spanning across 59 different families and categorized them into 9 distinct usage groups. Among these, Astragalus oihorensis, Astragalus owirensis, Cicer nuristanicum, Geranium parmiricum, and Rochelia chitralensis stand out as novel species with distinctive applications. Notably, medicinal use garnered 600 reports, while animal feed, veterinary applications, human consumption, and toxicity recorded 500, 450, 425, and 104 reports, respectively. Informant consensus was high ranging between 0.8 and 0.9 with most agreement on human food and animal feed category. Platanus orientalis and Juglans regia, with RFC 0.91, were the most cited. The Family Importance Value (FIV) of Juglandaceae and Platanaceae, each with an FIV of 0.91, and Capparidaceae with an FIV of 0.83 indicate the intricate role the families play.ConclusionsIn this study, we explore 150 ethnobotanical species, uncovering novel entries within ethnobotanical literature. Among these, several species showcase unique uses previously undocumented in Pakistani literature. Our research sheds light on the intricate interaction between plants and the distinct cultural landscape of the Lotkuh region.

From acute food insecurity to famine: how the 2023/2024 war on Gaza has dramatically set back sustainable development goal 2 to end hunger

The widespread destruction and the devastating humanitarian toll caused by the ongoing war on Gaza have transformed this besieged Strip into a place of death and despair. This review will explore the implications of this war for food security, focusing on Sustainable Development Goal (SDG) 2, which seeks to fight malnutrition and food insecurity and achieve zero hunger by 2030. This work is based on a review of grey literature, such as reports from government and non-governmental agencies, as well as recent scientific journal publications. Our results show that the ongoing war on Gaza has exacerbated the already acute food insecurity that Gazans have been struggling with since the blockade was imposed in 2007. Restless bombardment and ground operations have damaged or even razed agricultural land and all food production infrastructure (such as bakeries, mills, and food processing facilities), destroying Gaza’s food system. Facing catastrophic levels of hunger, some families, especially in northern Gaza have recently been resorting to eating animal feed and weeds to survive. With the starvation of civilians being used as a method of warfare, many experts and human rights organizations argue that Gaza is now the world’s worst hunger crisis and its population is on the verge of famine, if not already there. Moreover, this unprecedented humanitarian crisis in Gaza could have significant consequences on global food security in its six pillars, jeopardizing the implementation of SDG 2. While international organizations are making efforts to mitigate the catastrophic food shortage and famine, more comprehensive and sustainable solutions are needed to address the root causes of food insecurity in Gaza and ensure that all residents have access to an adequate and nutritious diet.

FORMATION OF BIOENEGRY BY FOOD WASTE

Food that has been tossed out. Nearly three-fourths of the waste generated by inhabitants of the maximum city in the past year comprised food, according to an environment status report (ESR) released by the BMC for 2019-20. According to BMC &amp; VVMC officials, the average waste generated per person per day is 250-650 grams. Waste management experts say if Mumbai recycles this waste, it could reduce the amount of garbage transported to its overburdened landfill. Food waste is an untapped energy source that mostly ends up rotting in landfills, thereby releasing greenhouse gases into the atmosphere. It’s better if we could somehow turn all the waste into clean energy and some other recyclable product. Food waste can be turned into valuable resources such as natural fertilizer, energy, and animal feed. Proper disposal of food waste has posed an environmental concern. also there is electric energy also can be made and the size of the dumping ground can be reduced. Also, we are going to make the all food waste management process digitalized so that Consumers can track the process of food waste management through the mobile application. The mobile application will help both consumers and as well as the government to maintain a record of their waste management, and it will be easy to take the record of the process, results, or how it can get improved. As we know the future generation will be mostly dependent upon renewable energy, so this type of food waste management process can save more energy for the future and will make a more human and animal-friendly nature/environment. So we can provide a good future for the next generations.

ALTERNATIVE NON-TRADITIONAL SOURCES OF FEED PROTEIN AND AMINO ACIDS

The article presents a literature review on the risks of the global problem of fodder protein and protein amino acids in the feeding of agricultural animals and poultry. Alternative ways of solving it are due to the production and use of non-traditional feed raw materials in feed diets. A number of scientists consider atypical agricultural biotechnological products obtained from insects to be one of the alternative sources of fodder protein and amino acids and see prospects in the development of their production. The most common subjects for study in this regard are crickets, locusts, silkworm larvae, flies, worms, etc. Their protein value, amino acid, fatty acid and mineral composition, the content of vitamins, and other biologically active substances are studied. The development of this direction is considered one of the ways to solve a number of urgent environmental and fodder problems on a biological basis. Рurpose of the research was to study the protein value and complete amino acid composition of flour from insects, namely flour from the larvae of the BSF fly, crickets and snails, and the methods of its determining: crude protein by the Kjeldahl method, digestible protein with the help of 0,2% pepsin, and the digestibility coefficient, and the principles of conducting researches, as well as the characteristics of methods for determining the content of replaceable and essential amino acids, including sulfur-containing, using the Kapel-105M capillary electrophoresis system. As a result of the research, it was found that cricket flour is the least valuable in terms of protein value (digestible protein and digestibility coefficient), and it is also poor in the content of nonessential and essential amino acids, compared to other studied types of insect flour. The best indicators of protein value were noted in the flour from BSF fly larvae in terms of the content of crude and digestible proteins, as well as the digestibility coefficient, which is 75.6%, which indicates its good digestibility by the bodies of animals and birds. The highest content of amino acids is contained in the flour from BSF fly larvae. Taking into account the above results of crude protein content, digestibility, and digestibility in this sample, amino acid digestibility will also be higher compared to other flour samples. In addition, flour from BSF fly larvae is rich in sulfur-containing amino acids. The content of tryptophan and histidine, compared to other types of raw materials, is the highest. The studied samples of insect flour contain a complete set of essential and nonessential amino acids, but BSF fly larvae flour and snail flour are more valuable for essential amino acids.

A novel family of defensin-like peptides from Hermetia illucens with antibacterial properties.

The world faces a major infectious disease challenge. Interest in the discovery, design, or development of antimicrobial peptides (AMPs) as an alternative approach for the treatment of bacterial infections has increased. Insects are a good source of AMPs which are the main effector molecules of their innate immune system. Black Soldier Fly Larvae (BSFL) are being developed for large-scale rearing for food sustainability, waste reduction and as sustainable animal and fish feed. Bioinformatic studies have suggested that BSFL have the largest number of AMPs identified in insects. However, most AMPs identified in BSF have not yet undergone antimicrobial evaluation but are promising leads to treat critical infections. Jg7197.t1, Jg7902.t1 and Jg7904.t1 were expressed into the haemolymph of larvae following infection with Salmonella enterica serovar Typhimurium and were predicted to be AMPs using the computational tool ampir. The genes encoding these proteins were within 2 distinct clusters in chromosome 1 of the BSF genome. Following removal of signal peptides, predicted structures of the mature proteins were superimposed, highlighting a high degree of structural conservation. The 3 AMPs share primary sequences with proteins that contain a Kunitz-binding domain; characterised for inhibitory action against proteases, and antimicrobial activities. An in vitro antimicrobial screen indicated that heterologously expressed SUMO-Jg7197.t1 and SUMO-Jg7902.t1 did not show activity against 12 bacterial strains. While recombinant SUMO-Jg7904.t1 had antimicrobial activity against a range of Gram-negative and Gram-positive bacteria, including the serious pathogen Pseudomonas aeruginosa. We have cloned and purified putative AMPs from BSFL and performed initial in vitro experiments to evaluate their antimicrobial activity. In doing so, we have identified a putative novel defensin-like AMP, Jg7904.t1, encoded in a paralogous gene cluster, with antimicrobial activity against P. aeruginosa.

Barley residue biomass, nutrient content, and relationships with grain yield

AbstractDetermination of barley (Hordeum vulgare L.) nutrient uptake in residue biomass is important for agronomic, economic, and environmental decisions. Improved understanding of grain yield, residue biomass, nutrient uptake, and their relationships are needed. Research determined these factors in 2018 and 2019 from trials of four barley classes (spring animal feed, human food, and malt, as well as winter malt), using three common cultivars of each, at five locations in southern Idaho. Production environment created the largest difference in residue biomass and nutrient uptake. Barley harvest index ranged from 0.46 to 0.52 Mg Mg−1 across feed, food, and malt classes. Compared to previous estimates, nutrient concentrations from the combined dataset were greater than for N, less than for P, and greater than for K. Correlation of grain yields to nutrient uptake (excluding Cu and Fe) had r2 ranging from 0.68 to 0.89. At current prices, economic analysis indicated that fertilizer replacement costs for total residue biomass nutrients were greater than previous estimates and could greatly exceed current sale value. These relationships and value estimates can be used to improve prediction of barley residue biomass production and nutrient uptake to guide best management practices. The decision of how to utilize these metrics (on‐farm, regional, etc.) should be considered based on known variation in measured nutrient and residue data and considered in relation to the proposed task.

Exploring the Genetic Wealth of Sunflower (Helianthus annuus L.): A Comprehensive Review on Its Products and By-Products

Sunflower stands out as a significant oilseed crop, pivotal not only in the production of edible oil and biofuels but also in the formulation of nutritious animal feed. Particularly noteworthy are the diverse opportunities presented by sunflower by-products. From the nutrient-rich sunflower oil cake to the versatile sunflower wax utilized in cosmetics and pharmaceuticals, the potential applications are manifold. Additionally, the burgeoning interest in sunflower-derived biofuels as sustainable alternatives to conventional energy sources highlights the crop's growing importance in modern agriculture. However, to meet the evolving demands sustainably, an in-depth analysis of sunflower cultivation practices and nutrient management strategies for optimal productivity is imperative. This review provides a comprehensive overview of sunflower cultivation techniques, processing methods, and avenues for value addition across various sectors. Renowned for its vibrant flowers and seeds brimming with high-quality oil, sunflower holds a significant economic value. Its oil, rich in beneficial unsaturated fatty acids and essential vitamins, has garnered acclaim for its health benefits, including cholesterol reduction and cardiovascular health promotion. Thriving primarily in warm climates and exhibiting resilience to drought conditions, sunflowers have witnessed a surge in demand for their sprouts, roasted seeds, oil, and even agricultural tourism ventures. With sunflower seeds and oil serving as essential components in integrated production systems, the scope for creating diverse food and non-food products continues to expand. By offering this comprehensive synthesis, this review aims to guide stakeholders towards maximizing the utilization of sunflowers across multiple domains.

In Vitro Evaluation of Gastrointestinal Stability of Pediococcus pentosaceus Isolated from Fermented Maize and Pearl Millet for Possible Novel Chicken Probiotic Development

Research has identified certain bio-based products, such as probiotics, as alternatives to antibiotics for use in animal feed. They are capable of controlling, preventing or minimizing the colonization of the gastrointestinal tract by pathogenic bacteria. To isolate Pediococcus spp. and assess its technological properties for possible probiotic development, maize and pearl millet were used. The cereals were steeped and wet milled after 48 h of fermentation. The milled cereals were kneaded into dough for 24 h, after which a 10% slurry was prepared for tenfold serial dilution to enumerate the LAB by employing pour plate techniques using MRS Agar. Based on the cell morphology of the isolated bacteria, eight isolates (four from maize and four from millet) that were selected for identification using MALDI-TOF MS showed that five were Pediococcus pentosaceus (P. pentosaceus), one was Pediococcus acidilactici, and two did not match any organism. Subsequently, the six isolates were labeled as MZ1, MZ2, MZ3, MZ4 for the maize isolate and MLT5 and MLT7 for the millet isolate. The six Pediococcus spp. were assessed in vitro for acid and bile salt tolerance, gastric juice and intestinal fluid tolerance and antibiotic resistance and antimicrobial susceptibility tests were performed, and the survivability rate of the strains was calculated. With regard to the mean count, there was a reduction in log10 CFU/mL under the lower pH conditions and their duration of exposure with regard to time. Among the isolates, no differences were noted at the various periods of exposure (0 h, 1 h, 2 h and 3 h) at pH 4 (p &gt; 0.05). However, significant differences were noted at pH 3, 2 and 1 among the isolates (p &lt; 0.05). The percentage survival of MZ4 and MLT7 at pH 1 was higher compared to the other isolates at 0 h. Significant differences were observed among the isolated at pH 2, 3 and 4 across the various periods. The mean count of the isolates in gastric juice was similar at 0 and 1 h, but significant differences were noted at 2 and 3 h, where MLT7 was highest (p &lt; 0.05). A similar trend was observed for percentage survival. The mean count and the percentage survival of isolates under different concentrations of bile salt were similar. Significant differences were noted among isolates in both mean count and percentage survival when exposed to intestinal fluid (p &lt; 0.05). All of the isolates were highly tolerant to the antibiotics tested and possessed antibacterial properties against the selected pathogens. The LABs proved to be good probiotic materials, according to the results obtained. However, the Pediococcus strain MLT7 proved to be the LAB of choice; therefore, its molecular identity was verified using the 16S rRNA sequence and was labeled as Pediococcus pentosaceus GT001 after it was discovered to have 100% similarity with some strains of Pediococcus pentosaceus.

High concentrations if fructose and NaCl affect choice behavior in larval drosophila

As a crucial adaptation for survival, the gustatory system serves as a primary mechanism for distinguishing between nutrient-rich compounds that are palatable and those that may pose risks or be unpleasant. This intricate sensory apparatus not only guides behavioral choices but also underpins flavor recognition across various species. Understanding the complexities of the gustatory system in Drosophila larvae has profound implications for unraveling the complexities of human gustatory perception as well as advancing our understanding of sensory processing mechanisms more broadly, thereby providing a broader repertoire of the topic for current days. Behavioral studies employing diverse stimuli have been instrumental in elucidating the underlying processes governing choice, feeding, and learning behaviors in animals. Building upon this foundation, our study sought to explore the specific effects of elevated concentrations of fructose and NaCl on the choice behavior of Drosophila larvae. Through meticulously designed assays using 3M concentrations of fructose and NaCl, we unequivocally demonstrated the pronounced preference of larvae for fructose over NaCl. Furthermore, the robustness of this preference was reaffirmed through a secondary assay employing 3M fructose and water as a control. Our comprehensive findings not only highlight the significant influence of elevated concentrations of fructose and NaCl on larval choice behavior but also shed light on the intricate interplay between sensory perception and genetic mechanisms, particularly involving the Gr5a gene. These insights contribute to our broader understanding of the role of the gustatory system in shaping crucial behaviors vital for animal survival, with potential implications extending to human sensory perception research and beyond.

An up-scaled biotechnological approach for phosphorus-depleted rye bran as animal feed

Side streams from the milling industry offer excellent nutritional properties for animal feed; yet their use is constrained by the elevated phosphorus (P) content, mainly in the form of phytate. Biotechnological P recovery fosters sustainable P management, transforming these streams into P-depleted animal feed through enzymatic hydrolysis. The enzymatic P mobilization not only enables P recovery from milling by-products but also supports the valorization of these streams into P-depleted animal feeds. Our study presents the scalability and applicability of the process and characterizes the resulting P-depleted rye bran as animal feed component. Batch mode investigations were conducted to mobilize P from 100 g to 37.1 kg of rye bran using bioreactors up to 400 L. P reductions of 89% to 92% (reducing from 12.7 gP/kg to 1.41–1.28 gP/kg) were achieved. In addition, High Performance Ion Chromatography (HPIC) analysis showed complete depletion of phytate. The successful recovery of the enzymatically mobilized P from the process wastewater by precipitation as struvite and calcium hydrogen phosphate is presented as well, achieving up to 99% removal efficiency. Our study demonstrates a versatile process that is easily adaptable, allowing for a seamless implementation on a larger scale.Graphical

Rodent and broiler feeding studies with maize containing genetically modified event DP-915635-4 show no adverse effects on health or performance

Several regulatory agencies continue to require animal feeding studies to approve new genetically modified crops despite such studies providing little value in the safety assessment. Feeding studies with maize grain containing event DP-915635-4 (DP915635), a new corn rootworm management trait, were conducted to fulfill that requirement. Diets fed to Crl:CD®(SD) rats for 90 days contained up to 50% ground maize grain from DP915635, non-transgenic control, or non-transgenic reference hybrids (P1197, 6158, and 6365). Ross 708 broilers received phase diets containing up to 67% maize grain from each source for 42 days. Growth performance was compared between animals fed DP915635 and control diets; rats were further evaluated for clinical and neurobehavioral measures, ophthalmology, clinical pathology, organ weights, and gross and microscopic pathology, whereas carcass parts and select organ yields were determined for broilers. Reference group inclusion assisted in determining natural variation influence on observed significant differences between DP915635 and control groups. DP915635 maize grain diet consumption did not affect any measure evaluated in either feeding study. Results demonstrated DP-915635-4 maize grain safety and nutritional equivalency when fed in nutritionally adequate diets, adding to the existing literature confirming the lack of significant effects of feeding grain from genetically modified plants.

A sustainable bio-circular way for biorefinery of rice straw into bioproducts based on energy-efficient pretreatment

Lignocellulosic biorefinery faces challenges associated with high energy input during pretreatment and high enzyme cost for enzymatic hydrolysis of cellulose, as well as the intricate valorization of lignin and hemicellulose. This study applied KOH-urea pretreatment to obtain maximum lignin removal of 68.93 % from rice straw at 80 °C for 60 min. The pretreatment consumed only 10.49 % of the total energy input. Five-time dilution of neutral black liquor can optimally stimulate the plant growth. Enzymatic conversion of KOH-urea-treated rice straw attained 92.02 % at 20 % solid concentration with 10 FPU cellulase/g substrate. The ethanol concentration achieved 37.02 g/L at a yield of 75.89 %. The solid residue after fermentation contained 42.40 % dietary fiber and 16.70 % protein, which is suitable as animal feed. This study developed a simple bio-circular way to concurrently produce liquid fertilizer, bioethanol, and animal feed from rice straw.

Market-driven land use in the Legal Amazon

The impact on ecosystems resulting from the introduction of productive activities is a consensus worldwide. However, some analyses have veered away from scientific rigor in favor of other interests. This phenomenon becomes evident when considering productive activities within the Amazon Biome. The diversity in soil structure and vegetation justifies this study and underpins its conclusions. We aimed to assess the impact of livestock farming and soybean cultivation in the state of Mato Grosso, which partially resides within the Amazon Biome. This analysis stems from the promotion of soybean production for animal feed due to the prohibition of meat and bone meal, a regulation that emerged following the Bovine Spongiform Encephalopathy (BSE) event. The results indicate that the prohibition of meat and bone meal in animal feed as a preventive measure against BSE transmission did not influence deforestation in the Legal Amazon in the analyzed region. Similarly, it did not affect the growth of the cattle herd, land income costs, variations in soybean prices in the international market, or beef prices in Brazil. Soybean cultivation did impact deforestation in the Legal Amazon. Government policies related to deforestation were effective in reducing encroachments on the Legal Amazon. Importantly, the most significant expansion of anthropogenic land use occurred within the Cerrado Biome of Mato Grosso rather than within the portion of the Amazon Biome.

An overview of alternative protein sources for ruminants in the tropical area

Abstract Investigating alternative protein sources for ruminants in tropical regions is crucial due to distinct climatic conditions and limited resources. Scientists, acknowledging the challenges, are exploring locally accessible alternatives to develop cost-effective and sustainable protein supplements for ruminant nutrition, emphasizing the importance of understanding the nutritional composition for advancing livestock husbandry sustainability. The aim of this research is to elucidate alternative local protein sources for ruminants in tropical areas and assess their effectiveness in promoting sustainable production and enhancing ruminant nutrition. Protein feed sources are essential for providing amino acids to animals and producing animal products such as meat, milk, and wool. Inadequate protein in animal feed may have a detrimental impact on rumen fermentation and animal output. Therefore, alternative protein sources have received increased attention in order to replace the main protein source. The alternative protein sources in this review were divided into five categories: agricultural biomass, fodder shrubs, biological protein and plant weeds, insect protein and agro-industry biomass. Furthermore, plant secondary compounds found in alternative protein sources might be used to modulate rumen fermentation and improve animal performance. By focusing on regional sources of protein, feed costs are reduced while availability is raised. Based on this analysis, it can be concluded that there are a number of alternative protein sources that might be used in place of primary protein sources and that they could support ruminant production in the face of several animal feed crises. However, the potential use of any protein source should be assessed in light of the country’s availability and animal production practices.

Phytochemical Analysis of Curry Leaf Extract (Murraya koenigii L.) as a Potential Animal Feed and Medicinal Ingredient

Phytochemical Analysis of Curry Leaf Extract (Murraya koenigii L.) as a Potential Animal Feed and Medicinal Ingredient

Evaluation of the feeding safety of Moringa (Moringa oleifera L.) in the Sprague Dawley rat

This study aimed to evaluate the safety of Moringa by comparing the effects of different gavage doses of Moringa. The general behavior, body weight, food intake, blood indexes, serum biochemical indexes, and histopathology of rats were used to determine the safety threshold and to provide a reference for the further development and use of Moringa as animal feed. 40 Sprague Dawley rats were selected and given transoral gavage for 28 consecutive days. The T1, T2 and T3 groups were observed for general behavior, body weight, and food intake. Blood and serum biochemical indices were quantified, and histopathology was performed to evaluate the effect and safety of Moringa. The results of the toxicological test showed that (1) Only T1 groups experienced diarrhea. (2) The body weight and food intake of rats in each group were normal compared with the control group. (3) The hematological and serum biochemical indices of rats in the T1 group were significantly different from those of CK but were in the normal range; (4) The results of microscopic examination of the heart, liver, spleen, lung, and kidney of rats in each group were normal, but inflammation occurred in stomach and jejunum of rats in the T1 group, but not in the ileum. The gastrointestinal tract of rats in the T2 and T3 groups were normal. (5) No abnormal death occurred in any of the treatment groups.The results of this study revealed that gavage of Moringa homogenate at a dose of 6 g/kg BW can cause diarrhea in rats. Although there is no pathological effect on weight, food intake, blood and serum biochemical indicators in rats, there are pathological textures in the gastrointestinal tissue caused by diarrhea. Therefore, the safety threshold of Moringa homogenate should be ≤ 3 g/kg BW.

Factors influencing small holder farmers adoption of climate SMART agriculture practices in Welmera Woreda, Central Ethiopia

Adoption of climate-smart agriculture practices are believed to have significantly lessened the devastating impact of climate change on agriculture. However, in countries like Ethiopia, the adoption and use level of climate-smart agriculture practices remains low. The understanding of farmers’ levels of CSA practice adoption and influencing factors is therefore crucial. The goal of the study is to evaluate the degree to which various CSA practices were being used in the study area, as well as adoption determinants. The study was conducted in Welmera district, Oromia, Ethiopia. Three kebeles were chosen from the district, and a random sample of 306 farmers was picked. We used a cross-sectional household survey, a focus group discussion, and interviews with key informants. A multivariate probit model was employed to investigate the factors influencing the adoption of multiple climate-smart agriculture practices. According to the result, conservation agriculture, integrated soil fertility management, and crop diversification are the most often used CSA practices. The results also revealed that male farmers outperformed female farmers in terms of crop diversity and improved animal feed and feeding practice adoption. The age of farmers has a considerable and unfavorable impact on their likelihood of adopting improved soil fertility management and crop diversification practices. However, it has a positive and considerable impact on the adoption of agroforestry practices. With regards to economic factors, having a relatively big farmland area considerably enhances the adoption of conservation agriculture, enhances soil fertility management and crop diversity, and improves livestock feed and feeding methods and post-harvest technology practice. Improved livestock feed and feeding are more likely to be used with higher farm income. Having a significant number of animals strongly promotes the adoption of conservation agriculture, and access to financial services positively impacts agroforestry, diversification of crops, and postharvest technology practice adoption. Furthermore, institutional factors including access to agricultural extension services and training were discovered to be important and beneficial for crop diversification; similarly, access to field day participation was discovered to have a significant and positive impact on the adoption of conservation agriculture and improved soil fertility management practices. It is critical to raise awareness about climate change among farmers and experts, as well as to incorporate location-specific CSA practices into agricultural programs.