Active Feedback Research Articles

Cell-Free Gene Expression in Bioprinted Fluidic Networks.

The realization of soft robotic devices with life-like properties requires the engineering of smart, active materials that can respond to environmental cues in similar ways as living cells or organisms. Cell-free expression systems provide an approach for embedding dynamic molecular control into such materials that avoids many of the complexities associated with genuinely living systems. Here, we present a strategy to integrate cell-free protein synthesis within agarose-based hydrogels that can be spatially organized and supplied by a synthetic vasculature. We first utilize an indirect printing approach with a commercial bioprinter and Pluronic F-127 as a fugitive ink to define fluidic channel structures within the hydrogels. We then investigate the impact of the gel matrix on the expression of proteins in E. coli cell-extract, which is found to depend on the gel density and the dilution of the expression system. When supplying the vascularized hydrogels with reactants, larger components such as DNA plasmids are confined to the channels or immobilized in the gels while nanoscale reaction components can diffusively spread within the gel. Using a single supply channel, we demonstrate different spatial protein concentration profiles emerging from different cell-free gene circuits comprising production, gene activation, and negative feedback. Variation of the channel design allows the creation of specific concentration profiles such as a long-term stable gradient or the homogeneous supply of a hydrogel with proteins.

Innovative approach of teaching ray-optics in the classroom

Abstract The present work discusses an innovative approach to teaching ray optics for students of class X. The school students were taught various concepts related to ray optics viz. Reflection, refraction, lateral displacement, mirrors, lenses, their types and terminologies, thin and thick lens, power of a lens, all cases of image formation in lenses, total internal reflection and its applications, human eye, its defects and corrections, making of telescope and microscope through practical demonstrations and hands-on approach using a self-conceived Ray Optics Kit. This activity was done independently in twenty two schools of Delhi, both government and private, with a massive total participation of 1200 students as part of SPIE Optics Outreach Activity. SPIE@ANDC Student Chapter members were trained at the college and they followed ‘Train the Trainer’ approach to create a pool of more than fifteen students who collectively conducted the workshops in teams in each of these schools. The student performance was assessed through a pre and post test as well as using a feedback form. An improvement of 65% in students’ performance was observed as indicated by comparing their pre-test and post-test results. The students as well as teachers gave a highly appreciative feedback of the whole activity. The feedback on their social media pages is a testimony of the success of the activity. Each school was awarded a Ray Optics Kit so as to make teaching and learning of ray optics interesting and engaging for batches in the future academic years.

Feasibility and Safety of a Home-based Electroencephalogram Neurofeedback Intervention to Reduce Chronic Neuropathic Pain: A Cohort Clinical Trial

ObjectiveTo evaluate the feasibility, safety, and potential health benefits of an 8-week home-based neurofeedback intervention. DesignSingle-group preliminary study. SettingCommunity-based. ParticipantsNine community dwelling adults with chronic neuropathic pain, 6 women and 3 men, with an average age of 51.9 years (range, 19-78 years) and with a 7-day average minimum pain score of 4 of 10 on the visual analog pain scale. InterventionsA minimum of 5 neurofeedback sessions per week (40min/session) for 8 consecutive weeks was undertaken with a 12-week follow-up baseline electroencephalography recording period. Main Outcome MeasuresPrimary feasibility outcomes: accessibility, tolerability, safety (adverse events and resolution), and human and information technology (IT) resources required. Secondary outcomes: pain, sensitization, catastrophization, anxiety, depression, sleep, health-related quality of life, electroencephalographic activity, and simple participant feedback. ResultsOf the 23 people screened, 11 were eligible for recruitment. One withdrew and another completed insufficient sessions for analysis, which resulted in 9 datasets analyzed. Three participants withdrew from the follow-up baselines, leaving 6 who completed the entire trial protocol. Thirteen adverse events were recorded and resolved: 1 was treatment-related, 4 were equipment-related, and 8 were administrative-related (eg, courier communication issues). The human and IT resources necessary for trial implementation were identified. There were also significant improvements in pain levels, depression, and anxiety. Six of 9 participants perceived minimal improvement or no change in symptoms after the trial, and 5 of 9 participants were satisfied with the treatment received. ConclusionsIt is feasible and safe to conduct a home-based trial of a neurofeedback intervention for people with chronic neuropathic pain, when the human and IT resources are provided and relevant governance processes are followed. Improvements in secondary outcomes merit investigation with a randomized controlled trial.

Development of Dual Cooperative Multi‐Urea Catalysts for Henry Reaction

AbstractA dual cooperative multi‐urea catalyst for Henry reaction was developed in which four urea groups were introduced into a rigid and rotatable framework. The tetraurea catalyst 1 showed higher activity for Henry reaction than its analogues, close‐bisurea 2 or distal‐bisurea 3 with two urea units, demonstrating additional cooperativity between the two substrate‐pinching catalytic sites of tetraurea 1. Addition of the reaction product to the reaction system resulted in a small amount of further activation. This work would provide a basis for new cooperative molecular catalysts, leading to a high degree of regulation based on allosteric cooperativity, e.g. with a distinct ON/OFF threshold for reactivity, or feedback activation by using the product as a reaction template.

A resource scheduling model for complex equipment development projects considering random overlapping and random feedback of activities

ABSTRACT Aiming to address the time and resource conflict problem caused by multiple tasks and frequent overlapping at each level in the process of complex equipment development, this article constructs a design structure matrix–graphical evaluation and review technique (DSM-GERT) hybrid model, takes the shortest duration of the project as the optimization objective and solves it using the genetic algorithm. Under the conditions of meeting the constraints of time, cost and quality, the project’s parameters are improved by adjusting the resource inputs. The study found that when project resources are insufficient, intertask messaging should be avoided as much as possible. If messaging is initiated, early messaging can be effective in reducing resource consumption, and decision makers can make trade-offs based on their preferences for resources.

Using expansive learning to design and implement video-annotated peer feedback in an undergraduate general education module

AbstractExisting studies have measured the effect of video-based feedback on student performance or satisfaction. Other issues are underacknowledged or merit further investigation. These include sociocultural aspects which may shape the design and implementation of video-based feedback, the ways students use technology to engage in feedback, and the processes through technology may transform learning. This study investigates the design and implementation of a video-annotated peer feedback activity to develop students’ presentation skills and knowledge of climate science. It explores how their use of a video annotation tool re-mediated established feedback practices and how the systematic analysis of contradictions in emerging practices informed the subsequent redesign and reimplementation of the approach. Employing a formative intervention design, the researchers intervened in the activity system of a first-year undergraduate education module to facilitate two cycles of expansive learning with an instructor and two groups of Hong Kong Chinese students (n = 97, n = 94) across two semesters. Instructor interviews, student surveys, and video annotation and system data were analysed using Activity Theory-derived criteria to highlight contradictions in each system and suggest how these could be overcome. The findings highlight the critical importance of active instructor facilitation; building student motivation by embedding social-affective support and positioning peer feedback as an integrated, formative process; and supporting students’ use of appropriate cognitive scaffolding to encourage their interactive, efficient use of the annotation tool. Conclusions: In a field dominated by experimental and quasi-experimental studies, this study reveals how an Activity Theory-derived research design and framework can be used to systemically analyse cycles of design and implementation of video-annotated peer feedback. It also suggests how the new activity system might be consolidated and generalised.

Single-nanoparticle electrophoretic mobility determination and trapping using active-feedback 3D tracking.

Nanoparticles (NP) are versatile materials with widespread applications across medicine and engineering. Despite rapid incorporation into drug delivery, therapeutics, and many more areas of research and development, there is a lack of robust characterization methods. Light scattering techniques such as dynamic light scattering (DLS) and electrophoretic light scattering (ELS) use an ensemble-averaged approach to the characterization of nanoparticle size and electrophoretic mobility (EM), leading to inaccuracies when applied to polydisperse or heterogeneous populations. To address this lack of single-nanoparticle characterization, this work applies 3D Single-Molecule Active Real-time Tracking (3D-SMART) to simultaneously determine NP size and EM on a per-particle basis. Single-nanoparticle EM is determined by using active feedback to "lock on" to a single particle and apply an oscillating electric field along one axis. A maximum likelihood approach is applied to extract the single-particle EM from the oscillating nanoparticle position along the field-actuated axis, while mean squared displacement is used along the non-actuated axes to determine size. Unfunctionalized and carboxyl-functionalized polystyrene NPs are found to have unique EM based on their individual size and surface characteristics, and it is demonstrated that single-nanoparticle EM is a more precise tool for distinguishing unique NP preparations than diffusion alone, able to determine the charge number of individual NPs to an uncertainty of less than 30. This method also explored individual nanoparticle EM in various ionic strengths (0.25-5 mM) and found decreased EM as a function of increasing ionic strength, in agreement with results determined via bulk characterization methods. Finally, it is demonstrated that the electric field can be manipulated in real time in response to particle position, resulting in one-dimensional electrokinetic trapping. Critically, this new single-nanoparticle EM determination and trapping method does not require microfluidics, opening the possibility for the exploration of single-nanoparticle EM in live tissue and more comprehensive characterization of nanoparticles in biologically relevant environments.

Synergistic atmosphere-ocean-ice influences have driven the 2023 all-time Antarctic sea-ice record low

Antarctic sea ice extent (SIE) reached a new record low in February 2023. Here we examine the evolution of the coupled ocean-atmosphere-sea ice system during the 12 months preceding the record. The impact of preceding conditions is assessed with observations, reanalyses, and output from the regional ocean-sea ice coupled model NEMO3.6-LIM3. We find that the 2022-2023 sea ice annual cycle was characterized by consistently low SIE throughout the year, anomalously rapid sea ice retreat in December 2022, and nearly circumpolar negative SIE anomalies in February 2023. While advection-induced positive air temperature anomalies inhibited the sea ice growth in most regions, strong southerly winds in the Amundsen-Ross Sea caused by an anomalously deep Amundsen Sea Low in spring transported notable volumes of sea ice northward, triggering an unusually active ice-albedo feedback onshore and favoring accelerated melt towards the minimum. This study highlights the impacts of multifactorial processes during the preceding seasons to explain the recent summer sea ice minima.

Renal autonomic dynamics in hypertension: how can we evaluate sympathetic activity for renal denervation?

This review explores the various pathophysiological factors influencing antihypertensive effects, involving the regulation of vascular resistance, plasma volume, cardiac function, and the autonomic nervous system, emphasizing the interconnected processes regulating blood pressure (BP). The kidney's pivotal role in BP control and its potential contribution to hypertension is complicated but important to understand the effective mechanisms of renal denervation (RDN), which may be a promising treatment for resistant hypertension. Excessive stimulation of the sympathetic nervous system or the renin-angiotensin-aldosterone system (RAAS) can elevate BP through various physiological changes, contributing to chronic hypertension. Renal sympathetic efferent nerve activation leads to elevated norepinephrine levels and subsequent cascading effects on vasoconstriction, renin release, and sodium reabsorption. RDN reduces BP in resistant hypertension by potentially disrupting sensory afferent nerves, decreasing feedback activation to the central nervous system, and reducing efferent sympathetic nerve activity in the heart and other structures. RDN may also modulate central sympathetic outflow and inhibit renal renin-angiotensin system overactivation. While evidence for RDN efficacy in hypertension is increasing, accurate patient selection becomes crucial, considering complex interactions that vary among patients. This review also discusses methods to evaluate autonomic nerve activity from the golden standard to new potential examination for finding out optimization in stimulation parameters or rigorous patient selection based on appropriate biomarkers.

How well do faculty do in providing general surgery EPA feedback?

IntroductionEntrustable Professional Activities (EPAs) provide a framework for competency-based assessment in surgery. EPA descriptions include observable behaviors by trainees at progressive levels of autonomy. The American Board of Surgery (ABS) required all General Surgery (GS) residency programs to implement assessment of 18 EPAs at the beginning of academic year 2023–2024. Microassessments provide formative self-reflection by the resident and feedback by faculty upon completion of the EPA. These frequent assessments culminate in a resident performance profile utilized by the trainee for formative growth and the clinical competency committee for summative feedback. Assessor free text comments are an opportunity to provide meaningful, constructive feedback to residents. Our aim was to analyze comments provided by faculty to residents in terms of their alignment with EPA descriptors and provision of actionable feedback. MethodsA total of 540 ​GS EPA assessments for inguinal hernia, gallbladder disease, appendicitis, trauma, and surgical consultation were evaluated from 6/2021-12/2022. We assessed free text EPA comments from faculty compared to EPA behavior descriptions for alignment with the selected EPA level of entrustment. The comments were judged on a binary scale of “Align” vs “Not Align” by two independent evaluators, with a third evaluator to address discordance. Comments were then evaluated for resident behavioral descriptions, suggestions for improvement, and positive or negative feedback. ResultsApproximately 77 ​% of EPA microassessments had alignment between level of autonomy and free text feedback. A common example of feedback discordant with level of autonomy was rating a trainee at an intraoperative level 4 (independent practice) with comments such as “required some guidance with retrocecal case and upsizing port.” Based on behavior descriptions this would be a level 3 (indirect supervision). Approximately 88 ​% of feedback contained positive comments with minimal negative feedback (e.g., “this did not go well.”). Actionable feedback including “work on optimization of retracting hand” or “continue to work clamp/tie technique and square off each knot” was present in 28.3 ​% of feedback. ConclusionsThe majority of faculty provide feedback that is aligned with the behavioral anchors of the EPAs assessed, but frequently did not provide actionable feedback to the resident regarding how to advance to the next level of entrustment. EPA entrustment behaviors provide a framework for the development of practice-ready behaviors, and if assessors anchor their feedback in the behaviors for a given entrustment level and project how a resident could proceed to the next level, they can provide a clear trajectory for skill development. Faculty development should focus on improving the frequency of actionable free text feedback, outlining how residents can advance in the future.

The Structural and Orbital Effects of Active Galactic Nuclei Feedback on SMBH Binaries Embedded in Gaseous Circumbinary Disks

Using subparsec-scale-resolution radiation+hydrodynamical adaptive mesh refinement simulations deployed with the RAMSES code, we study the dynamics of supermassive black hole (SMBH) binaries embedded in gaseous nuclear circumbinary disks, where we investigate the effects of active galactic nucleus feedback on the SMBH binaries' migration behavior and disk structure. The radiative feedback effects are modeled by injecting photons that interact with the gas, through the adoption of a grid of BH emission spectra. We run simulations with initial conditions that lead by pure gravity plus hydrodynamics both to the formation of a low-density tidal cavity and to systems where gas–viscous diffusion is efficient enough to maintain a sizable gas reservoir surrounding the binary. For gap-forming binaries we find that orbital evolution is unchanged with the inclusion of feedback, but ionizing radiation photoevaporates gas that is at the outer edge of the low-density region. For non-gap-forming systems we find that when feedback is included a strong initial disruption of the circumbinary disk is followed by an eventual stabilization of the medium that can usher a return to a fast binary migration regime. All of this is possible as a result of how our simulations capture the ionization states of the nuclear disk region and how this affects the coupling efficiency decrease with respect to the radiative feedback.

Mastering the Art of the Coaching Conversation

Abstract: This article provides a framework for leaders to conduct effective coaching conversations with employees. It outlines universal principles for planning and facilitating impactful coaching discussions. The framework consists of six key stages: setting the right environment and mindset by establishing rapport and confidentiality; jointly defining objectives and process parameters; delivering specific, actionable feedback with examples; allowing space for discussion of perceptions and self-assessment; collaboratively developing an action plan with goals and support; and closing positively with evaluation and appreciation. Empirical research and examples from Fortune 500 companies are referenced to validate strategies for feedback, goal-setting, self-reflection, and motivation. By strategically implementing this coaching model, the author argues leaders can foster continuous learning, improve performance, and help employees achieve their full potential.

Actionable adverse event monitoring and feedback to improve thoracic surgical care

Actionable adverse event monitoring and feedback to improve thoracic surgical care

Signatures of Active Galactic Nucleus Feedback Modes: A Green Bean Galaxy with 150 kpc Jet-induced Radio Emission

Jetted active galactic nuclei (AGN) hosting extended photoionized nebulae provide us with a unique view of the timescales associated with AGN activity. Here, we present a new green bean galaxy at z = 0.304458 ± 0.000007 with large-scale jet-induced radio emission. The spectral energy distributions of the radio components show steep spectral indices (α = −0.85 to −0.92 for the extended regions, and α = −1.02 for the faint radio core), and spectral age modeling of the extended radio emission indicates that the lobes are >6 Myr old. It is unclear whether the jet is active, or is a remnant with an off-time of 2–3 Myr. Several detached clouds lie around the host galaxy up to 37.8 kpc away from the nucleus, and their ionization profile indicates a decline (∼2 dex) in the AGN ionizing photon production over the past ∼0.15 Myr. Furthermore, we measure a blueshift for one of the clouds that is spatially coincident with the path of the radio jet. The cloud is likely illuminated by the photoionizing AGN, and potentially underwent an interaction with the relativistic jet. Our multiwavelength analysis suggests that RGB1 was in a phase of jet production prior to the radiatively efficient accretion phase traced by the detached cloud emission. It is unclear whether RGB1 transitioned into a low-excitation radio galaxy or an inactive galaxy over the past ∼0.15 Myr, or whether the extended radio and optical emission trace distinct accretion phases that occurred in succession.

Spatially Resolved Analysis of Stellar Populations in NGC 2992: Impact of Active Galactic Nucleus Feedback

In NGC 2992, a galaxy-scale ionized gas outflow driven by active galactic nuclei (AGNs) has long been recognized, yet its impact on the host galaxy has remained elusive. In this paper, we utilize data from the archival Very Large Telescope/MUSE to present a spatially resolved analysis of stellar populations in this galaxy. Two different stellar population templates are employed to fit the stellar continuum, allowing us to determine the light-weighted stellar age, metallicity, the fraction of the young stellar population (age <100 Myr, P Y), and the average age and metallicity of P Y. Our results reveal the presence of a very young stellar population (≤40 Myr) within the dust lane and nearly along the galaxy’s major axis. The light-weighted stellar age and the fraction of P Y show negative trends along the major and minor axes. The average age and metallicity of P Y present positive trends with increasing distance, except along the northern direction of the major axis. Within the circumnuclear region (<1 kpc), the distribution of the young stellar population is spatially anticorrelated with the AGN outflow cone. The highest fraction of P Y is observed at the outskirts of the nuclear radio bubble in the northern region near the nucleus. Considering the coupling efficiency and timescales, we propose that the AGN outflow in this galaxy may exert both negative and positive feedback on its host. Additionally, the star formation and the AGN activities could be attributed to the interaction between NGC 2992 and NGC 2993.

Knowledge and training among nursing students regarding the conspiracy of silence in palliative care: A participatory action research

AimTo develop and implement specific training based on the knowledge and management of conspiracy of silence among nursing students. BackgroundConspiracy of silence refers to the concealment of information from a patient on the family’s request, under the influence of a paternalistic culture that seeks to protect the patient. DesignParticipatory action research. MethodsWas conducted in the following stages: reconnaissance (focus groups); planning, action and observation (theoretical sessions); and reflection (analysis of care plans). The focus group consisted of six fourth year and eight second-year students. The intervention was conducted with 42 s-year students and a total of 93 s-year students participated in the resolution of the clinical case. The study was conducted between October 2022 and June 2023 at the Faculty of Nursing, University of Valencia. For data analysis, the process described by Carrillo et al. (2011) was followed, involving coding and the creation of categories and subcategories. ResultsThe focus group deficiencies were detected in the students' learning of palliative care competence, breaking bad news and the conspiracy of silence (reconnaissance stage). Therefore, an intervention was conducted to reinforce these knowledge areas, specifically addressing the conspiracy of silence (planning, action and observation stages). The resolution of the case showed how students with training approached the situation more comprehensively, including the family and proposed activities that were consistent with managing the situation (reflection stage). ConclusionsAn active feedback process was successfully established, where the students' feedback helped create specific training on oncological palliative care and provided the students with tools to manage the conspiracy of silence. The results underscore the importance of providing students with training in palliative care and managing conspiracy of silence, through therapeutic communication training, active training or enhancing emotional intelligence. This training is essential for cultivating the attitudes and skills required to deliver high-quality palliative care.

Conquering Communication Barriers: The English Quarantine Al Badriyah Program to Enhance Speaking Skills

Amidst the rapidly evolving global landscape, proficiency in English language skills has become indispensable for accessing information, facilitating effective communication, and engaging in global interactions. This necessity is particularly pronounced for students in Lombok who actively participate in diverse sectors such as agriculture, academia, and trade. Understanding the importance of English proficiency in these contexts, this study explores the communication barriers experienced by students during their learning process. It investigates the underlying factors that impede the development of their speaking skills. The qualitative study involved eighteen participants enrolled in the English Quarantine Al Badriyah (EQAB) Program, with eight selected as primary informants for detailed interviews. Data collection methods included closed-ended questions and semi-structured interviews, which were meticulously transcribed and analysed using data reduction, data display, and conclusion-drawing techniques. The research revealed several significant barriers hindering students’ English-speaking skills, including nervousness, lack of vocabulary, and shyness. Conversely, the study identified motivation factors, positive feedback and agreeable activities as pivotal elements that could effectively enhance students’ speaking proficiency. This study underscores the critical role of English language proficiency in empowering students to overcome barriers and actively participate in global discourse. By addressing these challenges and leveraging supportive factors, educators and stakeholders can better facilitate the development of robust English communication skills among students in diverse educational and professional settings.Keywords: Communication Barriers; Enhancing English; Speaking Skills.

The Research of the Disparity Between the Provision of Feedback Opportunities and the Clarity of Feedback Implementation for Business and Management Students at the University of Sussex and the University of Brighton

This research delves into the disparity between the provision of feedback opportunities and the clarity of feedback implementation for business and management students at the University of Sussex and the University of Brighton. Employing a qualitative constructivist approach, the study aims to uncover the barriers to student feedback integration and propose solutions for enhancing university responsiveness. The investigation reveals significant differences in feedback implementation rates and the perceived value of student opinions, with implications for teaching quality and student satisfaction. The study employs questionnaires, interviews, and focus groups to gather insights from students and staff, emphasizing the need for transparent communication and actionable feedback loops. The findings suggest that universities must prioritize student voices to foster a positive learning culture and improve institutional practices.

Targeting SOCS2 alleviates myocardial fibrosis by reducing nuclear translocation of β-catenin

BackgroundMyocardial fibrosis is an important pathological feature of dilated cardiomyopathy (DCM). The roles of SOCS2 in fibrosis of different organs are controversial. Herein, we investigated the function and potential mechanism of SOCS2 in myocardial fibrosis. MethodsBioinformatics, immunohistochemistry (IHC), immunofluorescence (IF), western blot (WB), real-time fluorescence quantitative PCR (qPCR), rat primary myocardial fibroblasts (rCFs) culture, doxorubicin (DOX) induced mouse dilated cardiomyopathy (DCM) model, and in vivo adeno-associated virus (AAV) infection were used to explore the role of SOCS2 in DCM. ResultsBioinformatics analysis showed that SOCS2 was positively correlated with fibrosis related factors. SOCS2 was significantly upregulated in patients and mice with DCM. In vivo experiments showed that targeted inhibition of cardiac SOCS2 could improve mouse cardiac function and alleviate myocardial fibrosis. Further research demonstrated that SOCS2 promoted the transformation of myofibroblasts. Knockdown of SOCS2 reduced the nuclear localization of β-catenin, which inhibited the fibrogenic effect of Wnt/β-catenin pathway. In addition, bioinformatics analysis suggested that lymphoid enhancer binding factor 1 (LEF1) was significantly positively correlated with SOCS2. Finally, dual luciferase assays demonstrated that LEF1 could bind to the promoter region of SOCS2, thereby mediating its transcriptional activation. ConclusionSOCS2 could activate the Wnt/β-catenin by regulating the nuclear translocation of β-catenin, which induces the transcriptional activation of SOCS2. Overall, these results indicated a positive feedback activation phenomenon between SOCS2, β-catenin and LEF1 in DCM. These results suggested that inhibition of SOCS2 could effectively alleviate the progression of myocardial fibrosis and improve cardiac function.

The effect of AGN feedback on the Lyman-α forest signature of galaxy protoclusters at z∼ 2.3

ABSTRACT The intergalactic medium in the vicinity of galaxy protoclusters are interesting testbeds to study complex baryonic effects such as gravitational shocks and feedback. Here, we utilize hydrodynamical simulations from the SIMBA and The Three Hundred suites to study the mechanisms influencing large-scale Lyman-$\alpha$ transmission in $2\lt z\lt 2.5$ protoclusters. We focus on the matter overdensity-Lyman-$\alpha$ transmission relation $(\delta _m-\delta _F)$ on Megaparsec-scales in these protoclusters, which is hypothesized to be sensitive to the feedback implementations. The lower density regions represented by the SIMBA-100 cosmological volume trace the power-law $\delta _m-\delta _F$ relationship often known as the fluctuating Gunn–Peterson approximation. This trend is continued into higher density regions covered by simulations that implement stellar feedback only. Simulations with active galactic nucleus (AGN) thermal and AGN jet feedback, however, exhibit progressively more Lyman-$\alpha$ transmission at fixed matter overdensity. Compared with the seven protoclusters observed in the COSMOS field, only two display the excess absorption expected from protoclusters. The others exhibit deviations: four show some increased transparency suggested by AGN X-ray thermal feedback models while the highly transparent COSTCO-I protocluster appears to reflect intense jet feedback. Discrepancies with the stellar-feedback-only model suggests processes at play beyond gravitational heating and/or stellar feedback as the cause of the protocluster transparencies. Some form of AGN feedback is likely at play in the observed protoclusters, and possibly long-ranged AGN jets in the case of COSTCO-I. While more detailed and resolved simulations are required to move forward, our findings open new avenues for probing AGN feedback at Cosmic Noon.