Feed Storage Research Articles

Dietary resveratrol alleviated lipopolysaccharide-induced ileitis through Nrf2 and NF-κB signalling pathways in ducks (Anas platyrhynchos).

Gram-negative bacteria contamination of feed can occur at all the stage of feed production, storage, transportation and utilization. Lipopolysaccharide (LPS) is a major toxic metabolite of Gram-negative bacteria. The aim of this study was to explore the effect of dietary resveratrol on the duck ileitis caused by LPS and its optimum addition level in diet. The results showed that LPS-induced duck ileitis with the destruction of intestinal structure, oxidative stress, mitochondrial dysfunction, inflammatory response and permeability alteration. Dietary resveratrol alleviated LPS-induced intestinal dysfunction and the increase of intestinal permeability by linearly increasing mRNA levels of tight junction protein genes (Claudin-1, Occludin-1, ZO-1) (p<0.05) and protein expression of Claudin-1 (p<0.01). In addition, dietary resveratrol improved the antioxidant capacity of duck ileum by reducing the production of MDA and increasing the activity of T-SOD (p<0.01) and CAT. Lipopolysaccharide increased Keap1 at mRNA and protein level (p<0.01) and decreased the protein level of Nrf2 (p<0.05). Dietary resveratrol significantly downregulated expression of Keap1 and upregulated expression of Nrf2 in duck (p<0.05). Dietary resveratrol suppressed the TLR4/NF-κB signalling pathway and the expression of its downstream genes including IKK, TXNIP, NLRP3, Caspase-1, IL-6 and IL-18. Meanwhile, the levels of inflammatory cytokines (IL-6, IL-18 and TNF-α) showed a linearly decrease (p<0.01) with increasing dietary resveratrol level. These results demonstrated that resveratrol alleviated the LPS-induced acute ileitis of duck through Nrf2 and NF-κB signalling pathways, and the dietary resveratrol of 500mg/kg is more efficiently.

Magnitude of Aflatoxigenic Aspergillus Species, Level of Aflatoxin B1, and Associated Factors in Stored Feed at Poultry Farms in Dire Dawa, Ethiopia.

Aflatoxin, the secondary toxic metabolite of Aspergillus species, particularly aflatoxigenic Aspergillus flavus and parasiticus, has a detrimental effect on poultry health and production. There exists some information gap about the magnitudes of aflatoxigenic Aspergillus species and aflatoxin in poultry feeds in the study area. Thus, the study was conducted to estimate the magnitude and assess the related potential factors of aflatoxigenic Aspergillus species with evaluations of the level of aflatoxin B1 in stored poultry feed at selected farms in Dire Dawa, Ethiopia. A cross-sectional study design was carried out on 374 poultry feed samples recruited by using a stratified simple random sampling technique. A pretested structured questionnaire was used to assess the level of knowledge and prevention practices associated with aflatoxin in poultry feed. The isolation of aflatoxigenic Aspergillus species was made by Aspergillus flavus parasiticus media, and aflatoxin B1 was estimated by aflatoxin B1 enzyme-linked immune sorbent assay. Results showed that the magnitude of aflatoxigenic Aspergillus species was 72.5% (95% CI: 67.6–76.9). The odds at which the species isolated were higher (p < 0.05) in feeds stored more than two months (AOR = 2.69), the presence of rodents in the storing room (AOR = 2.67), feeds having high moisture content (AOR = 1.5), and feed ingredient types (AOR = 4.3) compared to their counter parts. Only 34.4 and 32.8 percent of the respondents have better knowledge and apply prevention practice about fungal contamination and aflatoxin production in poultry feed, respectively. The occurrence of aflatoxigenic Aspergillus species in poultry feed was associated with the presence of rodents in the feed storing room with long storing period and high moisture contents of the feed. The knowledge and prevention practices employed by farm managers and workers about fungal contamination and aflatoxin in poultry feed are found low.

Feed and forage development in mixed crop–livestock systems of the Ethiopian highlands: Africa RISING project research experience

AbstractAfrica Research in Sustainable Intensification for Next Generation (RISING) project has conducted action research on livestock feed and forages starting in 2012 to bridge gaps on feed availability and quality. The objectives of this paper are to share approaches on how Africa RISING project enabled smallholder farmers to intensify feed and forage resources; present research findings of selected cultivated forages, fodder trees, and postharvest feed and forage utilization innovations; and highlight key lessons evolving from the feed and forage action research and scaling interventions. The project established partnership with CGIAR and local partners, conducted participatory need assessment, prioritized researchable issues, identified and validated different forages and utilization innovations, and created development partnership to facilitate wider scaling. Training activities and multistakeholder platforms were used to enhance cross learning of project partners. The cultivated forages grown under farmers’ fields and management conditions provided high biomass yield of good nutritional quality. For instance, oat (Avena sativa L.)–vetch (Vicia villosa Roth) mixture, lablab [Lablab purpureus (L.) Sweet], vetch–desho grass (Pennisetum pedicellatum Trin.) intercropping, sweet lupin (Lupinus albus L.), alfalfa (Medicago sativa L.), and fodder beet (Beta vulgaris L.) yielded on average 15, 3.4, 5.5, 2.9, 15.3, and 20.2 t dry matter ha−1, respectively. Animal response trials showed an increase in milk yield of 30–50% with supplementation of oat–vetch mixture and fodder beet. Feed wastage was considerably reduced (&gt;30%) due to the use of feed troughs and storage sheds, with high economic incentives to invest in such innovations. Integrating and intensifying feed and forage resources and postharvest innovations at farm level remains a priority to improve livestock productivity and food security.

Occurrence and associated factors of aflatoxin M1 in raw cow milk in South Gondar Zone, North West Ethiopia, 2020.

Aflatoxin M1 is the most significant toxin of milk and milk products. It is immunosuppressive, mutagenic, and carcinogenic compounds to humans. Therefore, this study was aimed to evaluate the concentration of aflatoxin M1 and its determinants in raw cow milk sample intended for human consumption in South Gondar Zone, Ethiopia. A cross‐sectional study was conducted on a total of 100 dairy farmers from January to February 2020. Around 50 ml, 100 raw milk samples were collected for aflatoxin M1 analysis. A simple random sampling technique was applied to get the households. Binary and multivariate logistic regressions were used to see the association between predictor and outcome variables. From the 100 dairy farmers who had participated, 38% had heard about aflatoxin in the milk sample. Aflatoxin M1 was detected in the 99(99%) raw milk samples, of these 41 (41%) exceeded the limit of the European Union. The logistic regression analysis result showed that residence, awareness about the level of aflatoxin in the milk sample, management mold‐contaminated animal feed, animal feed storage facility, and grazing systems were significantly associated with the high level of aflatoxin in the milk sample. Almost all milk samples analyzed were positive for aflatoxin M1, and 41% of samples were above the limit set by European Union. Many easily manageable and preventable factors were associated with higher levels of aflatoxin M1 in the milk sample than the European Union limit, which suggests continuous monitoring of milk and milk products is necessary.

Decision support system for integrated management of mycotoxins in feed and food supply chains

Mycotoxins present a global food safety threat of our feed and food. Mycotoxins are toxic metabolites of certain fungi in agricultural products that are harmful to animal and human health. The presence of mycotoxins in these products depends on a variety of management and environmental factors in the field, during storage and/or processing of feed and food commodities. To date, information on mycotoxin management is available, but is not easy to access by supply chain actors. This study aimed to design, build and test a Decision Support System (DSS) that can help decision making on mycotoxin management by various actors along the feed and food supply chains. As part of this, available knowledge and data on mycotoxin prevention and control were collected and synthesised into easy to understand guidelines and tools for various groups of end-users. The DSS consists of four different modules: (a) static information module and (b) scenario analysis module, (c) dynamic module for forecasting mycotoxins, and (d) dynamic module for real-time monitoring of moulds/mycotoxins in grain silos. Intended end-users are all end-user groups for modules (a) and (b); growers and collectors for module (c) and; post-harvest storage managers for module (d). The DSS is user-friendly and accessible through PCs, tablets and smartphones (seehttps://mytoolbox-platform.com/). In various phases of the DSS development, the tool has been demonstrated to groups of end-users, and their suggestions have been taken into account, whenever possible. Also, a near final version has been tested with individual farmers on the easiness to use the system. In this way we aimed to maximise the DSS uptake by actors along the chain. Ultimately, this DSS will improve decision making on mycotoxin management; it will assist in reducing mycotoxin contamination in the key crops of Europe, thereby reducing economic losses and improving animal and human health.

Detection of Stachybotrys chartarum isolates from faba beans dust during threshing.

Stachybotrys(S.)chartarumhad been related to dangerous health problems in animals and humans that take place when exposure to S.chartarum toxins.S. chartarumhad been isolated from various substrates, ranging from inappropriately stored feed and culinary herbs to damp buildings. To evaluate the pathogenic potential of isolates, it is essential to identify them with different methods. The occurrence and genetic diversity ofS. chartarum isolatesfrom faba beans dust during threshing in Upper Egypt were investigated. Low counts of Stachybotrys were found (six isolates) and identified morphologically by single-spore isolation and molecularly by the amplification of the specific internal transcribed spacer (ITS) region and glyceraldehydes-3-phosphate dehydrogenase (gpd).The genetic diversity of the collected isolates was studied by specific genes random primer polymerase chain reaction (SGRP-PCR). The phylogenetic analysis of S. chartarum showed that the specific primers IT51 and StacR3 used by commercial laboratories for detecting S. chartarum were not able to differentiate species of S. chartarum from S. chlorohalonata and unweighted pair group method of arithmetic averages (UPGMA) cluster analysis of SGRP fragments confirmed this result. The six isolates of S. chartarum were analyzed for the presence of trichodiene synthase (Tri5) gene, which needed in the early stage of the trichothecene synthesis path. All the tested isolates were positive for the Tri5 gene. Further study on the taxonomic status of the epithet S. chartarum is necessary and presence of sub species to S. chartarum might be acceptable depending on the variations of morphological characteristics which were confirmed by molecular techniques.

Application of plastics in livestock production: An overview

Plasticulture refers to use of plastics in agriculture in a scientific manner which not only improves the productivity but also optimizes the input resources. The plastic use in livestock farms is also becoming important, particularly for livestock products, for improving its keeping quality; livestock farm operations like storage of feed, feeding and watering devices; animal identification; milking machines and its tubings; milk cans; semen straws and AI sheaths, etc. Recently, the research work has been initiated for the usefulness of plastics in livestock shelter components like floor, wall partitions, protection panels, etc. under AICRP on PET project with ICAR-CIRG, Makhdoom and ICAR-NRCY, Dirang, Arunachal Pradesh for goats and Yaks, respectively as its animal science components. The main plastic products that are utilized on livestock farms are low density polyethylene (LDPE) and polypropylene (PP). This review paper discusses in brief about the plastic use in various stages of livestock production to marketing of its products.

Kualitas Tepung Ikan di Pesisir Pantai Kabupaten Merauke Sebagai Bahan Pakan

Catch fish that are not used as food can be processed into fish flour. The purpose of this study was to analyze fish meal from several types of fish found on the coast of Merauke RegencyThis test is carried out to see the physical and chemical quality.. From the research results of this study obtained the physical quality of fish meal can be which is categorized, both in mixing feed food and storage namely: the average density of 0.58 gr / cm3, surface area 53.88 cm2, pH 6.25, threshold power 1.15 m / sec and stack angle 27.260C, average ash content of 6.31%, protein 65.46%, fat 5.46%, crude fiber 0 and water content an average of 6.32%, These results indicate the quality of fish meal is included in standard I based on the quality of SNI 2013 as feed material. Eight (8) types of fish meal that have low economic value and potential to be developed as feed ingredients include Kaca fish (Kurtus gulliveri, Pasir fish (Platycephalus endrachtensis), Bete-Bete fish (Rhinoprenes pentanemus), Duri Herkules fish (Arius sp), and Duri putih fish (Arius leptaspis).

INVESTIGATION ASPECT OF TECHNICAL AND STRUCTURAL OF BEEF CATTLE BARNS IN ADIYAMAN PROVINCE

In this study, it was aimed to determine the current status and problems in terms of technical and structural conditions of beef cattle barns structures in Adıyaman city center and its districts, in the T.C. İpekyolu Development Agency (İKA) in TRC1 Region. The construction materials used in beef cattles, the dimensions of the structural elements, the levels of automation and mechanization, the product design and the problems faced by the manufacturers were determined. For this purpose, in line with the data obtained from Adıyaman provincial and district Agricultural Directorates, observations and measurements were made by going to all beef cattle enterprises and surveys were applied to each of the business owners to determine the technical and structural problems of the enterprises. According to the results of the survey, measurement, and observation, it was determined that all of the enterprises belong to the owners of the enterprises and all the shelters were planned as Open Beef Cattle Barns. 22.5% of the barns were built with the own money of the business owners, 5% by using loans, and 72.5% by getting both support and grant aid from the state. It was determined that 7,5% of beef cattle shelters had no roughage storage, 5% had no concentrated feed storage and 70% had no silage feed storage. As a result of the statistical analysis on beef cattle enterprises, a significant relationship was found between the construction years of cattle barns and needs to the renewal of barns or not (p&lt;0.01). In addition between the number of animals in shelters and utilization status from government supporting programs such as credit, support and grants of business owners, and between the number of animals in farms and the presence or not of manure storage in the farms, a significant relationship was determined (p&lt;0.01).

Integrated indicators for assessment of best management practices in tilapia cage farming

The aim of this study is to present APOIA-Aquaculture, an indicator system for assessing Best Management Practices (BMPs) in tilapia cage farming. Two specific objectives were set: (1) to detail the construction and operation of APOIA-Aquaculture; and (2) to attest to its analytical feasibility for BMP assessment in six fish farms selected as contrasting ventures at the Furnas and the Ilha Solteira reservoirs, important production sites in Southeastern Brazil. The proposed APOIA-Aquaculture comprises 68 integrated indicators, grouped into a set of four managerial dimensions: Spatial Organization (22 indicators), Management and Nutrition (23), Water Quality (14), and Quality of Sediment (9). The indicators were devised as to express fish farm compliance to predefined environmental standards, selected legislation requirements, and BMP effectiveness. Results of farm assessments indicate common deficiencies in the indicators of the Spatial Organization dimension, such as those related to the lack of equipment for water quality monitoring, fish cage positioning, improper storage of feed and supplements, inadequate control of fish disease symptoms, lack of appropriate records of the use of therapeutic and prophylactic products, and conflicts related to multiple water uses. In the Management and Nutrition dimension, indicators stressed the absence of biometric checks, inadequate procedures for residue disposal, and deficient control and records of fish losses (escapes). Overall, interactions were observed among indicators of the Management and Nutrition dimension and signs of deterioration in Water and Sediment Quality, as a consequence of inadequate feeding practices. Based on our results, we demonstrate the efficacy of APOIA-Aquaculture as an analytical tool for assessment of BMPs in tilapia cage farming. Aggregate information from indicators facilitates the adoption of measures to promote specific local infrastructure and monitoring needs according to proposed BMPs, towards improving the sustainability of the fish farms.

Dual pH responsive via double - layered microencapsulation for controlled release of active ingredients in simulated gastrointestinal tract: A model case of chitosan-alginate microcapsules containing basil oil (Ocimum basilicum Linn.)

Phytogenic feed additives (PFAs) have gained more attention to replace antibiotic growth promoters (AGPs) in poultry industry. However, the efficiency of PEAs might be lost during feed pelleting, storage and gastrointestinal (GI) tract resulting in an insufficient level at the small intestine. The present work demonstrates the use of double-layered polymers with opposite charges leading to a dual pH responsiveness where the encapsulated ingredients are remained active through the acidic gastric stage and ready to be released at the intestine. Anionic sodium alginate (SA) and cationic chitosan (CS) are a good combination to represent double-layered polymer of which an effective incorporation of active ingredient, i.e. basil oil (BO), a model PEA, can be achieved. By simply fabricating SA porous microcapsules followed by the absorption of BO before the surface coating with CS, the double-layered microcapsules, CS-SA, are obtained. The release performances confirm that CS-SA retains the antioxidant and antimicrobial activities of BO under storage in addition to a great tolerance of acids, bile, trypsin, including the thermal conditions. The present work, for the first time, proposes the concept of porous double-layered polymer in microcapsule form which shows the dual pH responsive for controlled release application. The use of ionic polysaccharides is simple, biocompatible and environmentally friendly, whereas the plausible upscaling for wide range of controlled release applications has to be further investigated.

Optimization of the process of compound feed pelleting for fur animals

Currently, fur farming products are becoming more and more in demand. With the increase in the number of fur-bearing animals, it is necessary to produce compound feeds that provide high quality furs with minimal feed costs. Compound feed recipes with a fat content of 20% and higher were developed for fur animals. Feeding fodder in the form of granules produced by the technology, one of the stages of which is the process of granulating compound fodders with a high fat content with reasonable operating parameters of the granulator press, is expedient. Process studies using the method of statistical planning of a multivariate experiment were carried out to determine the optimal granulation parameters. The following factors influencing the process under consideration were chosen: the amount of added fat, the steam consumption, the gap between the roll and the press die. Specific energy consumption and crumbling served as criteria for assessing the influence of the selected parameters, which make it possible to assess adequately the efficiency of the process. The data obtained showed that the process of pelleting compound feed at optimal parameters makes it possible to obtain pellets, the quality of which meets the requirements of the standard. All the data obtained were within the calculated confidence intervals of the optimization parameters. The change in the quality indicators of the obtained granulated compound feed during storage was also investigated in the work. Changes in the quality of granulated compound feed during storage testified that the values of total acidity and acid number of fat increased significantly after two months of storage due to hydrolysis of fat in conditions of free oxygen access. The value of the peroxide number of fat, which reflects the depth of oxidative changes in the compound feed, increased sharply after two months of storage. The content of vitamins A and E during the storage of compound feed for 45 days in summer was 12.3% and 10.5% of the initial values, respectively. The recommended shelf life of granulated feed with a high fat content in winter-spring period is 60 days, in summer - 45 days.

Second wave of COVID-19 pandemic and the surge of mucormycosis: Lessons learnt and future preparedness: Indian Society of Anaesthesiologists (ISA National) Advisory and Position Statement.

Second wave of COVID-19 pandemic and the surge of mucormycosis: Lessons learnt and future preparedness: Indian Society of Anaesthesiologists (ISA National) Advisory and Position Statement.

Conducting a comprehensive mycological study to prevent the occurrence of aspergillosis in chickens

Diseases of poultry aspergillosis are recorded in many poultry farms, the causes of the disease are the violation of the sanitary and hygienic conditions of hatcheries and poultry houses (unfavorable microclimate, affected feed and litter by microscopic fungi). To prevent the development of infection, it is necessary to control air pollution and equipment in hatcheries; determine the safety indicators of feed and water (the level of their mycological contamination) in poultry houses; monitor the status of feed storage facilities and water supply systems. According to the results of our research, in 15.1% of dead chickens at the age of 3 to 5 days and 6.6% at the age of 7-10 days, according to the results of mycological examination of pathological material, the diagnosis of aspergillosis was established. Aspergillus flavus (47%) Aspergillus fumigatus (34.5%), Aspergillus niger (10%) were isolated from the lungs of dead chickens. Macroscopically revealed edema and hyperemia of the lungs in chickens of 3-5 days of age. The formation of granulomas in the lungs and serous membranes was not detected, which may indicate an acute form of the pathological process. Chickens 7-10 days old showed a strong thickening of the air sacs, lungs with multiple spherical granulomas up to 2 mm in diameter. Spores of fungi entering the lungs cause a local inflammatory reaction - acute pneumonia. Histologically, in chickens of 3-5 days of age, a thickening of the parabronchial wall due to hyperplasia of local lymphoid formations (providing local immunity), signs of the development of interstitial pneumonia were revealed. Pathognomonic signs were found in the parenchyma - the formation of giant multinucleated cells, which is a characteristic sign of mycotic infections in poultry. The fusion of local pulmonary macrophages, histiocytes, into multinucleated cells is a prerequisite for the formation of granulomas, being a specific immune response in birds to the penetration of a pathogen. According to the development of the infectious process, caseous granulomas and fungal hyphae can be detected microscopically in the lungs. According to the results of histological examination, the formation of mycotic granulomas was found in the lung parenchyma in 7-10 days old chickens, which was accompanied by the formation of a zone of coagulation necrosis, in which the fungal hyphae were found. On the periphery, giant multinucleated epithelioid cells are located, the detection of which by histological examination is the basis for suspecting bird infection with microscopic fungi. Microscopic fungi Aspergillus flavus and Aspergillus fumigatus have been found in washes from hatchery ventilation cabinets, and Aspergillus flavus was found in the air from the chick sorting room and in washes from the feed mixer. Key words: aspergillosis, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, micromycete, mold fungi, histiocytes, granulomas, phylaids, conidia, hyphae.

The effect of extended storage on virus survival in feed

Extended feed storage to reduce the risk of virus survival has not been tested experimentally. Five ingredients inoculated with porcine epidemic diarrhea virus, porcine reproductive and respiratory syndrome virus-174, and Senecavirus A were stored indoors at 20°C or outdoors in Minnesota winter conditions. After 30 days, outdoor samples contained infectious virus, while indoor samples did not.

Expert Elicitation To Estimate the Feed Safety Impact of Criteria Included in the Canadian Food Inspection Agency Risk Assessment Model for Feed Mills

The Canadian Food Inspection Agency is developing an Establishment-based Risk Assessment (ERA) model for commercial and on-farm mills involved in the manufacture, storage, packaging, labeling, or distribution of livestock feed (ERA-Feed Mill model). This model will help inform the allocation of inspection resources on the basis of feed safety risk, including animal health and food safety risk. In a previous study, 34 risk factors, grouped into inherent, mitigation, and compliance clusters, along with assessment criteria were selected. The objective of this current study was to estimate the relative risk (RR) of the 203 assessment criteria on the basis of the impact on feed safety to design an ERA-Feed Mill model algorithm. Furthermore, the intent of this study was to assess the maximum increase or decrease of risk obtained when multiple criteria belonging to a same cluster were identified in a specific feed mill. To do so, a two-round face-to-face expert elicitation was conducted with 28 Canadian feed experts. Results showed no significant association between respondent profiles (years of experience and work sector) and estimated RR. Uniformity of answers between experts improved between rounds. Criteria having the highest increase in risk (median RR ≥ 4) included the presence of materials prohibited to be fed to ruminants in a facility that produces ruminant feed, the presence of multiple livestock species on-site, and historical noncompliances related to the inspection of the feed mill's process control and end-product control programs. Risk mitigation criteria having the highest impact on decreasing the risk were the implementation of feed safety certifications, the use of dedicated manufacturing lines (prohibited materials or medications), and having a hazard sampling plan in place for finished feed. The median RR assigned to each criterion and cluster will be used to build an algorithm of the Canadian Food Inspection Agency's ERA-Feed Mill model.

Biosecurity practices in commercial poultry farms located in ElFashir Locality- Sudan

This study was conducted to assess the biosecurity practices in commercial poultry farms in the locality of ElFashir, North Darfur State Sudan. Thirty two farms biosecurity tests were used, biosecurity practices were evaluated in poultry farms using the checklist and organized questionnaires administered to commercial poultry farmers. Simple descriptive statistics were used (frequencies and percentages) to summarize and display the results. The results showed that 62.5% of the farms used the floor brooding system of the birds; 59.4% open barns; The domestic stock in access to the farm site 68.8%; Pets or wildlife birds have access to inside of barns or feed storage 71.9%; yearly water quality is not checked by a reputable laboratory 96.9%; employees have routine contact with other poultry species 84.4%; regular cleaning and disinfection of feeders and drinkers washing is done mainly by soap in addition to water. 43.8%; employees and their families with other people on poultry housing site 100%; poultry production, feed consumption, and mortality records are kept, but not reviewed daily 18.8%; there is untrimmed vegetation and debris within barn(s) 81.2%; vehicle flow designated way traffic onto premises and off premises 100% and perimeter fencing and gate present but not always locked or guarded or fence not completed 56.25%. The study concluded that there is a severe weakness in the application of biosecurity practices in its three branches isolation, cleaning disinfecting and traffic control. The adopted hygiene and traffic control measurement were all within the high and medium biosecurity risk limits whereas mismanagement resembled 60.4% the highest risk of biosecurity practices among the studies in commercial poultry farms of ElFashir Locality. Therefore, it is strongly recommended for local authorities to intervene with qualified teams of poultry professional and experts to train poultry farmers, managers and attendees on importance of implementing sound biosecurity practices.

Impact of plasma irradiation on Tribolium castaneum

Radio frequency plasma (RFP) could provide reliable, compact, cost-effective irradiation applications against insect pests of stored food and feed products. Sensitivity of red flour beetle Tribolium castaneum to RFP has been investigated using an irradiation applicator system with the two types of inert gases, argon (Ar) and helium (He), at 100 W for five exposure time levels of 0, 20, 40, 60 and 90 s, respectively. We demonstrated that He RFP was more efficient against T. castaneum than Ar RFP. In addition, a positive correlation was observed between mortality percentages of treated insect stages and exposure times for both He and Ar RFP. The adult stage showed the highest tolerance to RFP irradiation followed by larvae and pupae; however, it was more susceptible than larvae within 24 h after He RFP treatments. The optimum exposure time was 90 s with He RFP, where a full mortality at all tested stages was accomplished, while mortalities of 71.4, 65.3 and 36.7% were recorded for pupae, larvae and adult stage, respectively, after an Ar RFP treatment of 90 s. In case of treated adults, the reproduction rate was higher than treated larvae and pupae. Our findings indicated that He RFP was an effective method for inhibiting T. castaneum development and impacting the insect life cycle and could be considered a promising tool for pest control of stored food.

TECHNOLOGICAL SOLUTION OF A MEAT FARM WITH EQUAL VEAL SUPPLY DURING A YEAR

The technological solution of a meat farm for 1000 cows of a broodstock with a three-round hotel of cows is presented. The first round is planned in january – march, the second round – in may – july and the third-from september to november. The goal of the development is to ensure regular production of beef during the year, obtained from the slaughter of 6–7-month-old steers and 8–9-month-old chicks, grown on suckling under mother cows to a live weight of 170–216 kg. The average daily growth of bulls – 800, heifers-700 g. the daily supply of meat will reach 62.4 c, an average of 2 c per day. The average level of profitability is 30.6%. New design and planning solutions for industrial buildings are proposed. The parameters of the feed zone of the farm and the need for in-farm equipment were determined. When developing the master plan of the farm, the following main zones were identified: production purposes, storage and preparation of feed, auxiliary production. For the use of loose keeping of animals, the structural scheme of buildings was chosen without internal supports. Keeping cows with calves in winter is loose on a deep non-replaceable litter. Unlike young animals, the delivery of adult animals for meat takes place in September – October. For the mechanization of production processes, there are two mixers, a mini-feed mill, auto-watering machines, ball drinkers with electric heating, a water dispenser, a straw roll shredder, and a machine for treating animals with a disinfectant solution.

Impact of a Novel Nano-Protectant on the Viability of Probiotic Bacterium Lactobacillus casei K17.

Probiotics are considered as desirable alternatives to antibiotics because of their beneficial effects on the safety and economy of farm animals. The protectant can ensure the viability of probiotics, which is the prerequisite of the beneficial effects. The objective of this study was to evaluate the effects of a novel nano-protectant containing trehalose, skim milk powder, phytoglycogen nanoparticles, and nano-phytoglycogen Pickering emulsions on the viability of Lactobacillus casei K17 under different conditions. The results indicated that the optimal concentration of the carbohydrate substrate was determined to be 10% skim milk powder (w/w) instead of trehalose. The combination of 10% skim milk powder (w/w), 1% phytoglycogen nanoparticles (w/w), and 10% Pickering emulsions (w/w) was selected as the optimal component of the protectant. Trilayer protectants with an optimal component had a more significant protective effect on the bacteria than that of the monolayer and bilayer protectants, or the control in feed storage, freeze-drying, and simulated gastrointestinal environment. A scanning electron microscope was used to monitor the morphological characteristics of the protectants for different layers on L. casei. In conclusion, the trilayer protectant exhibited a substantial effect on L. casei during storage and consumption, which could be used in the feed and functional food.