Feed Digestibility Research Articles

The effect of different energy sources on the performance and blood metabolites of sheep

Energy has an important role in metabolic processes. Dietary energy sources can be in the form of carbohydrates or fat. This study aimed to elaborate the performance and blood metabolites of ewes fed cassava, palm oil or canola oil. Sixteen ewes were divided into four groups of four treatments, namely T1 = 100% grass; T2 = 40% grass + 30% concentrate + 30% cassava; T3 = 40% grass + 50.3% concentrate + 9.7% palm oil; T4 = 40% grass + 50.3% concentrate + 9.7% canola oil. Randomized block design was used and data were analyzed using analysis of variance and any differences were furthered tested by Duncan multiple range test. The results showed that feeding with different energy sources significantly (P<0.05) had a significant effect on feed consumption and digestibility, weight gain, and feed utilization efficiency. The feed consumption of 100% grass ration (T1) was significantly higher than the other treatments. Diet with 30% cassava (T2) had significantly different feed digestibility and performance from the 100% grass and oil treatment. The blood cholesterol content of sheep fed with oil (T3 and T4) was higher than that given 100% grass (T1) and 30% cassava (T2). However, there were no differences among the treatment on blood glucose and protein concentration. It is concluded that the provision of 30% cassava resulted in better sheep performance and lower blood cholesterol levels compared to feeding with an energy source in the form of oil.

ОТБОР ПРОБ ПРИ ОЦЕНКЕ КАЧЕСТВА СМЕШИВАНИЯ

The improvement of the technological process of preparing dry loose feed mixtures is associated with an increase in the structure of feed rations for farm animals in the conditions of farms. It is noted that an increase in the digestibility and palatability of feed, a decrease in the specific energy consumption for the production of feed mixtures depends on the quality of their mixing. The methodological basis of the work was the results of studies of recent years by leading domestic scientists in the field of improving the technological processes for preparing feed mixtures, design and operating parameters of mixers. The analysis of sampling methods is given: periodic for batch mixers; cumulative, allowing to obtain a generalized sample, more representative of the mixing process, also applicable to the study of batch mixers; using special devices - samplers to obtain the most accurate samples. The importance of selecting a control component oriented to the functional features of the mixers, regardless of the sampling method used, is highlighted when monitoring the homogeneity of the mixture. The analysis of existing studies showed a wide range of control components, methods of sampling and separation of components in the study of the mixing process by machines of various designs and principles of operation. The analysis of the works showed that in order to reduce the time spent on isolating the control component and ensure the reliability of the studies, it is necessary to take into account the nature of the mixed cultures. When choosing seeds of sweet clover, clover, millet as control components, a sample mass of 5 grams is sufficient; seeds of beets, buckwheat, wheat and barley, a sample mass of 100 grams is sufficient; seeds of peas, corn, a sample mass of 300 grams is sufficient. A sieve classifier with optimal selection of the mixture with the possibility of separating in sieve or sieve analysis is noted as the most applicable for the isolation of the control component. It is recommended to focus on the method of periodic sampling when examining batch mixers. The cumulative sampling method using samplers is recommended for investigating the performance of continuous mixers and evaluating the overall quality of mixing in batch mixers.

Effect of Alfalfa (Medicago Sativa L.) Hay Supplementation and Urea Molasses Block on Feed Intake, Digestibility, and Body Weight Change of Yearling Local Sheep Fed Grass Hay as Basal Diet

A study was conducted to evaluate the effect of supplementation of alfalfa (Medicago sativa L.) hay and urea molasses block on feed intake, digestibility, and body weight change of yearling local sheep fed with grass hay as a basal diet. The rationale for supplementation of alfalfa hay with urea molasses block 1) high-producing animals protein need cannot exclusively met from rumen microbial sources; 2) protein is the most expensive nutrient of ruminant animal feed. Twenty-four yearling intact male local sheep with a mean initial body weight of 23.9±1.9 (Mean ± SD) were used in a completely randomized block design. Treatments were 1% of live weight alfalfa hay (1A), 1.5% of live weight alfalfa hay (1.5A), 2.0% of live weight alfalfa hay (2A), and all three treatments offered with 100g/head/day of Urea-molasses-block (UMB). The control treatment was supplemented with concentrate mix at 2.0% of live weight (C). In addition, all treatments were fed grass hay ad libitum. The study period consisted of 84 days of feeding and 7 days of digestibility tests. Total dry matter intake was 1015.65, 925.41, 956.16, and 1078.13 (SE=± 19.4) for C, 1A, 1.5A, and 2A, respectively and 1A was lower than 2A and similar with 1.5A and C. Apparent crude protein digestibility and Crude protein intake of 2A was statistically similar to C. But, higher than 1A and 1.5A. Average daily gain was 109.33, 54.76, 55.36, and 63.1 (SE=± 6.13) g/day for C, 1A, 1.5A, and 2A, respectively and the values were the highest for C. The study showed that concentrate supplementation at 2% body weight resulted in greater body weight gain, crude protein intake and apparent crude protein digestibility. Additionally, the weight gain achieved at all grade levels of alfalfa plus in combination with the same amount of urea molasses block was commendable. But, considering the accessibility and cost of conventional concentrate mixture, 2% of Live Weight Alfalfa hay with 100g/head/day urea molasses block is recommended as an alternative supplement regime for better sheep performance.

Beneficial impacts of biochar as a potential feed additive in animal husbandry

In the last decade, biochar production and use have grown in popularity. Biochar is comparable to charcoal and activated charcoal because it is a pyrogenic carbonaceous matter made by pyrolyzing organic carbon-rich materials. There is a lack of research into the effects of adding biochar to animal feed. Based on the reviewed literature, including its impact on the adsorption of toxins, blood biochemistry, feed conversion rate, digestion, meat quality, and greenhouse gas emissions, adding biochar to the diet of farm animals is a good idea. This study compiles the most important research on biochar's potential as a supplement to the diets of ruminants (including cows and goats), swine, poultry, and aquatic organisms like fish. Biochar supplementation improves animal growth, haematological profiles, meat, milk and egg yield, resistance to illnesses (especially gut pathogenic bacteria), and reduced ruminant methane emission. Biochar's strong sorption capacity also helps efficiently remove contaminants and poisons from the animals' bodies and the farm surroundings where they are raised. Animal farmers are predicted to make greater use of biochar in the future. Biochar could potentially be of value in the healthcare and human health fields; hence research into this area is encouraged. The present review highlights the potential benefits of biochar as an additive to animal feed and demonstrates how, when combined with other environmentally friendly practices, biochar feeding can extend the longevity of animal husbandry.

Modulation of Murrah Buffalo (Bubalus bubalis) Rumen Functions for In Vitro Fatty Acid Bio-Hydrogenation, Methane Production and Fermentation Pattern of Total Mixed Ration Supplemented with Allium sativum (Garlic) Essential Oils

The potential for plant-origin essential oils to modulate rumen functions for reducing bio-hydrogenation of fatty acids and methane production has been a significant area of research in recent times. This study investigated the effects supplementation of garlic (Allium sativum) essential oils have on in vitro bio-hydrogenation of fatty acids, methanogenesis and fermentation characteristics of total mixed ration in buffalo with the aim of enhancing conjugated linoleic acid (CLA) content in animal products as well as reducing environmental pollution. Allium sativum (AS) essential oils were examined at four levels [0 (Control), 33.33 µL (AS-1), 83.33 µL (AS-2) and 166.66 µL (AS-3) per litre of buffered rumen fluid] in a radio-frequency based automatic gas production system (ANKOM-RF). Two bottles per treatment per run over two incubation runs were undertaken to gain representative results. Oats hay and concentrate mixture (1:1) was used as a substrate (500 ± 5 mg) and incubated with 60 mL of buffered rumen fluid in 250 mL ANKOM bottles fitted with automatic an gas recording system at 39 °C for 24 h, following standard in vitro gas production protocols. The results demonstrated a reduction (p &lt; 0.01) in lipid bio-hydrogenation, measured by lowered saturated fatty acids and enhanced unsaturated fatty acids on the supplementation of AS essential oils, irrespective of the dose levels. Moreover, the increased (p &lt; 0.01) production of trans vaccenic (trans C18:1) acid (TVA) following graded dose supplementations of the AS essential oils increased the production of conjugated linoleic acids (CLA) in animal products. Although, reduced methane production (p &lt; 0.01) was evidenced, the decrease in total gas production and feed digestibility (TDDM) demonstrated the strong antimicrobial properties of AS at all dose levels. The study reveals that the Allium sativam (Garlic) essential oils have the potential to be an agent for the reduction of the rumen biohydrogenation of fatty acids and methanogenesis. However, in vivo examination is necessary to validate the findings and confirm its suitability for use as an additive to enhance nutraceutical and organoleptic properties in animal products.

Effects of Long-Term Grazing on Feed Intake and Digestibility of Cattle in Meadow Steppe

(1) Estimation of grazing livestock intake is the basis for studying animal–plant relationships and the nutritional status of grazing livestock and has important implications for grassland composition and productivity. (2) We used the saturated alkanes method to determine the feed intake and vegetation nutrient digestibility of livestock at different grazing intensities and in different months. (3) We found that C31 had the highest concentration in both pasture and fecal output, and the average recovery of C31 was 77.99%. The different grazing intensities significantly affected livestock intake. As the grazing intensity increased, there was a decreasing trend of livestock intake and the highest livestock feed intake was 6.11 kg DM/day in light grazing. With the increase in grazing season months, the highest livestock intake was 6.67 kg DM/day in the cold period in September. The month also had a significant effect on the digestibility of livestock for all nutrient variables when compared to the grazing intensity. Livestock weight and medium palatability species are more important for livestock intake. (4) Our study provides a more accurate measurement of grazing livestock intake, which can be used as a reference for the scientific management of grazing livestock and the rational use of grazing pastures.

Peningkatan Protein Black Soldier Fly (BSF) Untuk Pakan Ternak Sebagai Hasil Biokonversi Sampah Makanan

Based on the data obtained, 1.3 billion tons of food waste are generated annually worldwide. This amount constitutes one-third of the food successfully produced for human consumption. And Indonesia has succeeded in becoming the second largest producer of food waste in the world. In this study, observations will be made of the effect of the mixture composition on food waste originating from Jambangan TPS on BSF larvae bioconversion. The research was conducted on a field scale with a running time of 12 days. Data analysis carried out included waste reduction index, efficiency conversion of digestive feed, biomass weight gain, compost yield testing and protein content in larvae. Compost results will be compared with SNI 19-7030-2004 concerning Compost Specifications from Domestic Organic Waste. And the results of the protein content in the larvae will be compared with SNI (8290.1: 2016) concerning laying hen feed (production period) and with conventional chicken feed. The results showed that the highest percentage of reduction ability was from the food waste and amino acid reactor which managed to reach 80%, and the final weight of the larvae touched 0.2-0.3 gr. The laboratory test results stated that the compost and protein content complied with the existing SNI. Meanwhile, the palatability test states that fresh maggot has the best value. But the drawback of fresh maggot is that it can cause damage to the digestive tract, besides that it also has low shelf life..

Effects of Cyperus alternifolius, Echinochloa pyramidalis, Typha angustifolia, and Imperata cylindrica on Growth Performance, Feed Digestibility, Gut Microbiota, Haemato-biochemical and Immunity Parameters in Broiler Chickens

The rhizomes of Cyperus (C.) alternifolius, Echinochloa (E.) pyramidalis, Typha (T.) angustifolia, and Imperata (I.) cylindrica are rich in secondary metabolites and have diverse pharmacological activities. The present study was designed to evaluate the effects of dietary C. alternifolius, E. pyramidalis, T. angustifolia, and I. cylindrical rhizomes on the performance of broiler chickens. A total of 384 day-old chicks were randomly assigned to six treatment groups (each treatment replicated four times). The first group received a basal diet (negative control), and the second group received a basal diet with 1 gr of antibiotic (Doxicycline, positive control). Other groups received a basal diet with 2 gr of each phyto-additives/kg feed. The results revealed that treatments had no significant effects on feed intake and carcass yield in chickens. The C. alternifolius and T. angustifolia significantly increased live weight and weight gain, and decreased feed conversion ratio, compared to negative control. The addition of C. alternifolius, T. angustifolia, and I. cylindrica to broilers’ diet significantly increased the apparent digestibility of dry matter and crude protein, compared to the negative control. Compared to the negative control, the lactic acid bacteria count significantly increased with the incorporation of T. angustifolia and I. cylindrica. The granulocytes count and globulins concentration were not affected by the different treatments. However, the lymphocyte count was significantly decreased with the diet containing E. pyramidalis compared to the negative and positive controls, and the diets containing C. alternifolius and T. angustifolia. The spleen and bursa weights and volumes significantly increased in all groups of chickens fed on phyto-additives, compared to the negative control. Except for haematocrit, which significantly increased with C. alternifolius and T. angustifolia in the treatments compared to the negative control, the feed additives did not significantly affect the hematological parameters. Compared to the negative control, T. angustifolia and I. cylindrica significantly increased HDL-cholesterol concentration in broiler chickens’ serum, while all treatment groups were comparable for all the other biochemical parameters. Incorporating 2 g of C. alternifolius and T. angustifolia in broiler chickens’ feed improves feed digestibility, enhances the population of lactic acid bacteria in the gut, and causes subsequent improvement in growth performance.

The effects of fumaric acid on in vitro true digestibility of tea wastes produced with different cultivation methods

This study aims to determine the effects of adding different fumaric acid (FA) levels to tea factory wastes (TFW) produced by different cultivation methods on in vitro true digestibility. In vitro true digestibility of feed (IVTDAs feed), dry matter (IVTDDM), organic matter (IVTDOM), and neutral detergent fibre (IVTDNDF) were performed with a Daisy Incubator. Fumaric acid did not add to the control group and added 0.1%, 0.2%, or 0.3% FA to the experimental groups. When the cultivation methods were compared (conventional and organic tea wastes), it was seen that FA made a significant difference (P0.05). There was a significant difference (P

DETERMINATION OF YIELD AND QUALITY CHARACTERISTICS OF COMMON REED (Phragmites australis (Cav.) Trin. Ex Steud) HARVESTED AT DIFFERENT GROWTH STAGES

Common Reed (Phragmites australis (Cav.) Trin. Ex Steud, which is remained inactive and grown in saline&#x0D; drainage channels, has been seen as an important potential to reduce the roughage deficit needed in animal nutrition. For this purpose, some yield, fermantation, chemical and nutritional characteristics of P.australis herbage and silage obtained from the end of the vegetative stage (EVS), the beginning of panicle (BP) and the full panicle stage (FPS) were determined. The study was carried out in 2021-2022 in Igdir, which is located in the North-East of Turkey. The results showed that the forage yield characteristics increased as the development period progressed and also that high lactic acid, low pH, acetic, propionic and butyric acid contents desired in silo feeds were obtained from the cuttings at FPS. In addition, ammonia production was low in silages at BP and FPS, but high the fleig score. It was observed that the dry matter digestibility, metabolic energy and relative feed value were higher while dry matter and crude protein ratio, acid detergent fibre and crude ash content of herbage according to silage were lower. On the other hand, the desired chemical and nutritional values in both herbage and silage were determined during the most suitable BP. As a result, it was determined that the common reed was appropriate to harvest in FPS for a silo feed with high yield and fermentation properties. In addition, it was concluded that the herbage and silage harvested at BP produced a higher quality feed material in terms of nutritional and chemical composition, respectively.

The effect of various additives on the microbiota of ruminant rumen and the course of metabolic processes in the rumen &lt;i&gt;in vitro&lt;/i&gt; studies

Relevance. In an in vitro study, changes in the taxonomic scar and the course of fermentation processes in response to the addition of various lipid additives to the ruminant diet were studied.Methods. Three samples were formed: control — without the addition of oil, the experimental ones were supplemented with sunflower oil (3%) (I) and soy (3%) (II). The object of the study is the scar content obtained from Kazakh white-headed bulls with chronic scar fistula (n = 3) by the Latin square method. Studies of the digestibility of SV were carried out using the incubator «ANKOM DaisyII» according to a specialized technique. Laboratory studies: the level of volatile fatty acids (VFA) in the contents of the scar was determined by gas chromatography on a gas chromatograph «Crystallux-4000M», determination of nitrogen forms according — to GOST 26180-84. Microbial biodiversity of the rumen contents was carried out using MiSeq («Illumina», USA) by the new generation sequencing method (NGS) with a set of reagents MiSeq® Reagent Kit v3 (600 cycle).Results. The addition of oils had a stimulating effect on the digestibility of SV feed in the rumen, increasing this indicator by 2.7%. Compared with the control, the addition of oil reduced the concentration of total and protein nitrogen in the scar content, while the content of non-protein nitrogen increased (by 5.4%) when using sunflower and (by 7.8%) soybean oil. The additional inclusion of oils showed an increase in the level of acetic and butyric acids, respectively: in the group using sunflower oil — by 46.8% and 55.6%, in the group with soybean oil — by 51.5% and 60.5% relative to the control. The additional inclusion of sunflower oil in the diet showed a change in the number of microorganisms in the RYE, but the qualitative composition of the microbiota relative to the control group did not change significantly.

Digestibility of Feed Supplemented with Common Pleco Meal and Its Impact on The Growth of Nile Tilapia (Oreochromis sp.)

Common pleco is a wild fish whose habitat occupies a lot of reservoir areas and public waters in Indonesia. The amount is abundant and not consumed by humans, is a great potential to be used as a source of animal protein in fish feed. The purpose of this study was to evaluate the digestibility of common pleco fish meal in artificial feed for nile tilapia (Oreochromis niloticus.) The study was carried out at the Aquaculture Laboratory Hatchery, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, while the manufacture of common pleco meal, proximate analysis of feed ingredients and the test feed was carried out by the Laboratory of Chemistry and Animal Feed, Faculty of Animal Husbandry, Universitas Padjadjaran. The design used in this study was a Completely Randomized Design (CRD) consisting of 5 (five) treatments and 3 (three) replications. The treatment given was the addition of common pleco flour in artificial feed at 0%, 5%, 10%, 15% and 20%. The parameters observed in this study consisted of digestibility, growth rate, and water quality. The results of the research on some of these parameters indicate that the addition of common pleco meal can still be used in artificial feed up to 10%. The tilapia studied had the highest relative growth with a value of 0.218%. The digestibility level of the studied feed reached a value of 85.256%, and the water quality studied was still within the safe tolerance limits for fish farming. Based on the results of this study, the negative effect on the survival rate of tilapia did not occur until the addition of common pleco flour to artificial feed reached 10%. Thus, the fish meal has the potential as an alternative to fish meal in fish feed formulations.

Assessment of the Multidimensional Performances of Food Waste Utilization Technologies in China

In recent years, China has made great efforts to carry out source-separation in municipal solid waste management; in particular, the utilization of food waste has received increasing attention. Food waste-utilizing technologies, including anaerobic digestion, aerobic biological treatment, and insect feed conversion, are currently applied in China. However, in the existing cases, there were several disadvantages, such as a low rate of utilization, significant environmental impacts, poor economic benefits, etc., as well as a lack of systematic analysis and comprehensive assessment of the performances of food waste utilization technologies. In this study, a four-dimensional performance assessment method (resource efficiency, environmental impact, economic feasibility, and social effect, with 21 indicators) was established for assessing the life cycle process of food waste utilization technologies. We collected detailed information of 14 typical food waste utilization cases in China, and the results showed that the average scores of anaerobic digestion and insect feed conversion were 58.39 and 59.65, higher than those of the aerobic biological treatment (49.16). The highest scores among all subdivision technologies were centralized black soldier fly conversion and mesophilic wet anaerobic digestion, which were 67.14 and 60.82, respectively. With respect to the technologies of different treatment capacities and distribution modes, i.e., centralized and decentralized technologies, the resource efficiency and economic benefit scores of the former were 13% and 62% higher than those of the latter, whereas the environmental impacts and social effect scores of the latter were 8% and 34% greater than those of the former, respectively. In practice, the utilization technology of food waste should be chosen according to local conditions, such as the physical and chemical properties of food waste, the classification of municipal solid waste, financial affordability, collection and transportation distance, etc.

Influence of probiotics on growth dynamics and blood parameters of Zaanen goats

Relevance. In the prevention and treatment of gastrointestinal diseases in the rearing of young animals, the restoration of intestinal biocenosis with the use of probiotics — bacterial preparations from live microbial cultures is of great importance.The purpose of the research is to study the effect of probiotic feed additives Klyuver Pro and BeneFIT Basic on growth dynamics, feed costs and blood parameters of Saanen goats.Methods. The studies were carried out at LLC «Lukoz Saba» of the Sabinsky district of the Republic of Tatarstan. The object of the study is the kids of the Saanen breed at the age of seven days. Scientific and economic experience was carried out according to the method of analogues. The chemical composition and nutritional value of feed, the dynamics of changes in live weight, the morphological and biochemical parameters of the blood of goats were studied. Statistical data processing was carried out using Microsoft Excel. A difference of p ≥ 0.05 was considered significant.Results. Probiotic feed additives Kluver Pro and BeneFIT Basic activate metabolic processes in the body of kids, increase the digestibility and assimilation of feed, which has a positive effect on the growth and development of animals, and reduce feed costs. The live weight of goats treated with probiotics increased (compared with the control) by 7.7% (p ≥ 0.05) and 3.6%. Feed costs decreased per 1 kg of live weight gain decreased by 13.6% and 10.1%.In the blood of animals treated with probiotics, there was an increase in hematocrit, hemoglobin and erythrocytes. In animals of the second group, these indicators increased by 17.4%, 31.7 g/l (39.3%) (p ≥ 0.001) and 4.45 ˟ 1012/l (77.1%) (p ≥ 0.001), in animals the third group — respectively, by 7.5%, 7.7 g / l (9.5%) (p ≥ 0.001) and 1.63 ˟ 1012/l (28.2%), as well as a significant decrease in ESR — by 0, 37 mm/g and 0.18 mm/g (p ≥ 0.001), calcium content by 20% and 24.2% (p ≥ 0.05).

Review: Diving into the cow hologenome to reduce methane emissions and increase sustainability.

Interest on methane emissions from livestock has increased in later years as it is an anthropogenic greenhouse gas with an important warming potential. The rumen microbiota has a large influence on the production of enteric methane. Animals harbour a second genome consisting of microbes, collectively referred to as the "microbiome". The rumen microbial community plays an important role in feed digestion, feed efficiency, methane emission and health status. This review recaps the current knowledge on the genetic control that the cow exerts on the rumen microbiota composition. Heritability estimates for the rumen microbiota composition range between 0.05 and 0.40 in the literature, depending on the taxonomical group or microbial gene function. Variables depicting microbial diversity or aggregating microbial information are also heritable within the same range. This study includes a genome-wide association analysis on the microbiota composition, considering the relative abundance of some microbial taxa previously associated to enteric methane in dairy cattle (Archaea, Dialister, Entodinium, Eukaryota, Lentisphaerae, Methanobrevibacter, Neocallimastix, Prevotella and Stentor). Host genomic regions associated with the relative abundance of these microbial taxa were identified after Benjamini-Hoschberg correction (Padj<0.05). An in-silico functional analysis using FUMA and DAVID online tools revealed that these gene sets were enriched in tissues like brain cortex, brain amigdala, pituitary, salivary glands and other parts of the digestive system, and are related to appetite, satiety and digestion. These results allow us to have greater knowledge about the composition and function of the rumen microbiome in cattle. The state-of-the art strategies to include methane traits in the selection indices in dairy cattle populations is reviewed. Several strategies to include methane traits in the selection indices have been studied worldwide, using bioeconomical models or economic functions under theoretical frameworks. However, their incorporation in the breeding programmes is still scarce. Some potential strategies to include methane traits in the selection indices of dairy cattle population are presented. Future selection indices will need to increase the weight of traits related to methane emissions and sustainability. This review will serve as a compendium of the current state of the art in genetic strategies to reduce methane emissions in dairy cattle.

Pemanfaatan Tepung Lemna sp. dan Enzim Non-Starch Polysaccharides (NSPs) Pada Pakan Ikan Tambakan (Helostoma temminckii)

Lemna sp. is one of the local raw materials that can be used to substitute fish flour and soybean meal in fish feed formulation because its protein contain. However, protein plant-based in fish feed formulation need additional enzyme (exogenous) such as Non starch polysaccharides (NSP) enzyme which able to increase feed digestibility. The aim of this study is to determine the effect of NSP enzyme to Lemna sp. flour in feed formulation for kissing gouramy’s growth and feed efficiency. This research has been conducted at the Experimental Pond Laboratory, Aquaculture Study Program and Laboratory of Chemical and Microbiology of Agricultural Products, Department of Agricultural Technology, Faculty of Agriculture, Sriwijaya University. Juvenile kissing gourami 4±0,5 cm in length were used and culture in certain condition which was temperature 25-27 °C, pH 6, dissolved oxygen &gt; 2.5 mgL-1 and ammonia &lt;0.1 mgL-1. The Rovabio Excel AP10 T-Flex were used as NSP enzyme. Completely Randomized Design (CRD) was used with 4 treatments (Tn) and 3 replications, namely the addition of Lemna sp. flour 0% without enzyme (T0), addition of Lemna sp. flour 0% with enzyme (T1), addition of Lemna sp. flour 5% without enzyme (T2), addition of Lemna sp. flour 5% with enzyme (T3). NSP enzyme dose is 1 g/kg feed material. The results showed that T3 gave the best results with absolute weight growth 0.91 g, absolute length growth 0.63 cm, specific weight growth rate 1.35% day-1, specific length growth rate 0, 41% day-1, survival 100%, and feed efficiency 28.59%.

Compartment 1 acidosis associated with hemorrhagic diathesis causing mortalities among racing camels in Oman.

Camels are adapted to digestion of dry rough forages for their nutrition, and sudden change to highly digestible feed during the racing season causes digestive disorders. The current study investigated the cause of death among racing dromedary camels within 3-7 days following a sudden onset of fever ≈ 41 °C, colic with tarry feces, and enlarged superficial lymph nodes. Marked leukopenia, low RBC count and thrombocytopenia, deranged liver and renal function tests, and prolonged coagulation profiles were reported. Compartment 1 fluid revealed a pH of 4.3-5.2 with absence or few ciliated protozoa and Gram-positive microbial flora. Widespread petechial to ecchymotic hemorrhages were observed in various organs including the gastrointestinal tract (compartment 3 and colon), lungs, and heart. Fibrin thrombi in arterioles, capillaries, venules, and medium-sized veins were observed especially in the pulmonary interstitium, submucosa of the large intestine (ascending colon), deep dermis, and renal cortex. Furthermore, widespread hemorrhages and necrosis were constant histopathological lesions in parenchymatous organs. Based on clinical signs, hematology, blood biochemistry, and gross and microscopical findings, the cases were diagnosed as compartment 1 acidosis associated with hemorrhagic diathesis and endotoxicosis. Finally, compartment 1 acidosis associated with hemorrhagic diathesis is a serious fatal disease on the Arabian Peninsula in racing dromedaries causing multi-organ dysfunction and coagulopathy and disseminated hemorrhages.

Effect of the Structure of Highly Porous Silica Extracted from Sugarcane Bagasse Fly Ash on Aflatoxin B1 Adsorption.

Sugarcane bagasse fly ash is industrial waste produced by incinerating biomass to generate power and steam. The fly ash contains SiO2 and Al2O3, which can be used to prepare aluminosilicate. This latter material exhibits high potential as an adsorbent in various applications, including the livestock industry where issues related to contamination of aflatoxins in animal feeds need to be addressed; addition of adsorbents can help decrease the concentration of aflatoxins during feed digestion. In this study, the effect of the structure of silica prepared from sugarcane bagasse fly ash on physicochemical properties and aflatoxin B1 (AFB1) adsorption capability compared with that of bentonite was investigated. BPS-5, Xerogel-5, MCM-41, and SBA-15 mesoporous silica supports were synthesized using sodium silicate hydrate (Na2SiO3) from sugarcane bagasse fly ash as a silica source. BPS-5, Xerogel-5, MCM-41, and SBA-15 exhibited amorphous structures, while sodium silicate possessed a crystalline structure. BPS-5 possessed larger pore size, pore volume, and pore size distribution with a bimodal mesoporous structure, while Xerogel-5 exhibited lower pore size and pore size distribution with a unimodal mesoporous structure. BPS-5 with a negatively charged surface exhibited the highest AFB1 adsorption capability compared with other porous silica. However, the AFB1 adsorption capability of bentonite was superior to those of all porous silica. Sufficient pore diameter with high total pore volume as well as high intensity of acid sites and negative charge on the surface of the adsorbent is required to increase AFB1 adsorption in the in vitro gastrointestinal tract of animals.

Effect of substitution of poultry by-product meal by bovine by-product meal on overall performance for Litopenaeus vannamei

The effect of partial to the total substitution of poultry by-product meal for bovine by-product meal (BBM) was evaluated under fish-free basis diets for the white shrimp Litopenaeus vannamei. For this purpose, four isoproteic (46.5%) and isolipidic (8.5%) diets without any fish meal and fish oil added, different inclusion levels of BBM were included (0%, 13.6%, 27.2%, and 40.7%) equivalent to Control, L-BBM, M-BBM, and H-BBM, were evaluated. In triplicate groups, three hundred and sixty shrimp were randomly distributed (0.47 g ± 0.006) in 12 ponds (30 shrimp per unit). After seven weeks of experimentation, the biological indices, hemolymph chemistry, apparent digestibility (%), and fatty acid profile were evaluated. It was observed that the H-BBM treatment improved biological indices compared to the Control. In addition, significant differences were found, such as higher final weight (2.66 ± 0.13), weight gain (2.2 ± 0.1), and specific growth rate (3.4 ± 0.1). In addition, the lower feed intake observed in the H-BBM resulted in lower feed conversion (1.5 ± 0.12) than the Control group (2.8 ± 0.12). On the contrary, in survival, the H-BBM treatment presented the lowest percentage (88.9 ± 4.6), while the control diet had the highest survival (100%). Regarding the apparent digestibility (%), it was observed that the H-BBM treatment presented the highest percentage (91.5 ± 0.3). At the same time, the Control group had the lowest (85.8 ± 0.6), finding significant differences among treatments with BBM compared to the Control group. In addition, a low protein concentration was observed in the M-BBM treatment for hemolymph chemistry. At the same time, bovine meal showed significant differences among treatments, particularly in the H-BBM treatment, resulting in higher cholesterol and triglycerides levels. Therefore, it is concluded that BBM is a protein source with a high prospect of being included in shrimp diets, resulting in better benefits in its performance with higher feed conversion efficiency and digestibility. However, further research on its effect on lipid metabolism is recommended.

Early life supplementation with a natural blend containing turmeric, thymol, and yeast cell wall components to optimize rumen anatomical and microbiological development and productivity in dairy goats

Ruminants are born with an anatomically, microbiologically, and metabolically immature rumen. Optimizing the rearing of young ruminants represent an important challenge in intensive dairy farms. Therefore, the objective of this study was to evaluate the effects of dietary supplementation of young ruminants with a plant extract blend containing turmeric, thymol, and yeast cell wall components such as mannan oligosaccharides and β-glucans. One hundred newborn female goat kids were randomly allocated to 2 experimental treatments, which were unsupplemented (CTL) or supplemented with the blend containing plant extracts and yeast cell wall components (PEY). All animas were fed with milk replacer, concentrate feed, and oat hay, and were weaned at 8 wk of age. Dietary treatments lasted from wk 1 to 22 and 10 animals from each treatment were randomly selected to monitor feed intake, digestibility, and health-related indicators. These latter animals were euthanized at wk 22 of age to study the rumen anatomical, papillary, and microbiological development, whereas the remaining animals were monitored for reproductive performance and milk yield during the first lactation. Results indicated that PEY supplementation did not lead to feed intake or health issues because PEY animals tended to have a higher concentrate intake and lower diarrheal incidence than CTL animals. No differences between treatments were noted in terms of feed digestibility, rumen microbial protein synthesis, health-related metabolites, or blood cell counts. Supplementation with PEY promoted a higher rumen empty weight, and rumen relative proportion to the total digestive tract weight, than CTL animals. This was accompanied with a higher rumen papillary development in terms of papillae length and surface area in the cranial ventral and caudal ventral sacs, respectively. The PEY animals also had higher expression of the MCT1 gene, which is related to volatile fatty acid absorption by the rumen epithelium, than CTL animals. The antimicrobial effects of the turmeric and thymol could explain the decreased the rumen absolute abundance of protozoa and anaerobic fungi. This antimicrobial modulation led to a change in the bacterial community structure, a decrease in the bacteria richness, and to the disappearance (i.e., Prevotellaceae_UCG-004, Bacteroidetes_BD2-2, Papillibacter, Schwartzia, and Absconditabacteriales_SR1) or decline of certain bacterial taxa (i.e., Prevotellaceae_NK3B31_group, and Clostridia_UCG-014). Supplementation with PEY also decreased the relative abundance of fibrolytic (i.e., Fibrobacter succinogenes and Eubacterium ruminantium) and increased amylolytic bacteria (Selenomonas ruminantium). Although these microbial changes were not accompanied with significant differences in the rumen fermentation, this supplementation led to increased body weight gain during the preweaning period, higher body weight during the postweaning period, and higher fertility rate during the first gestation. On the contrary, no residual effects of this nutritional intervention were noted on the milk yield and milk components during the first lactation. In conclusion, supplementation with this blend of plant extracts and yeast cell wall component in early life could be considered as a sustainable nutritional strategy to increase body weight gain and optimize the rumen anatomical and microbiological development in young ruminants, despite having minor productive implications later in life.