Feeding Soybean Research Articles

Influence of feeding thermally peroxidized soybean oil on growth performance, digestibility, gut integrity, and oxidative stress in nursery pigs.

The objectives of the current experiments were to evaluate the effect of feeding soybean oil (SO) with different levels of peroxidation on lipid, N, and GE digestibility, gut integrity, oxidative stress, and growth performance in nursery pigs. Treatments consisted diets containing 10% fresh SO (22.5 °C) or thermally processed SO (45 °C for 288 h, 90 °C for 72 h, or 180 °C for 6 h), each with an air infusion of 15 L/min, with postprocessing peroxide values of 7.6, 11.5, 19.1, and 13.4 mEq/kg and p-anisidine values of 1.92, 6.29, 149, and 159, for the 22.5 °C, 45 °C, 90 °C and 180 °C processed SO, respectively. In experiment 1, 64 barrows (7.1 ± 0.9 kg initial BW) were randomly allotted into 2 rooms of 32 pens and individually fed their experimental diets for 21 d, with a fresh fecal sample collected on day 20 for determination of GE and lipid digestibility. In experiment 2, 56 barrows (BW 9.16 ± 1.56 kg) were placed into individual metabolism crates for assessment of GE, lipid, and N digestibility and N retention. Urinary lactulose to mannitol ratio was assessed to evaluate in vivo small intestinal integrity, and urine and plasma were collected to analyze for markers of oxidative stress. Pigs were subsequently euthanized to obtain liver weights and analyze the liver for markers of oxidative stress. In experiment 1, pigs fed the SO thermally processed at 90 °C had reduced ADG (P = 0.01) and ADFI (P = 0.04) compared to pigs fed the other SO treatment groups, with no differences noted among pigs fed the 22.5 °C, 45 °C, and 180 °C SO treatments. No effects of feeding thermally processing SO on dietary GE or lipid digestibility (P > 0.10) were noted in either experiment. In experiment 2, there was no dietary effect of feeding peroxidized SO on the DE:ME ratio, N digestibility, or N retained as a percent of N digested, on the urinary ratio of lactulose to mannitol, on serum, urinary, or liver thiobarbituric acid reactive substances, on plasma protein carbonyls, or on urinary or liver 8-OH-2dG (P > 0.10). In experiment 2, pigs fed the SO thermally processed at 90 °C had the greatest isoprostane concentrations in the serum (P ≤ 0.01) and urine (P ≤ 0.05) compared to pigs fed the unprocessed SO. These results indicate that the change in fatty acid composition and/or the presence of lipid peroxidation products in peroxidized SO may reduce ADG and ADFI in nursery pigs, but appears to have no impact on GE, lipid, or N digestibility, or gut permeability. These data suggest that the presence of lipid peroxidation products may affect certain markers of oxidative stress.

Effect of soybean feeding on productivity of sows, growth of piglets and quality of pork

Effect of soybean feeding on productivity of sows, growth of piglets and quality of pork

Effects of dietary lipid sources on growth performance and carcass traits in Pekin ducks

This study was conducted to determine the influence of dietary lipid sources on growth performance, carcass traits and taste scores in Pekin ducks. A total of 1,500 fifteen-day-old ducks (820 ± 22 g) were blocked based on body weight (BW), and randomly allotted to 3 treatments with 10 replicates of 50 birds each (25 males and 25 females). The experiment lasted for 4 wk, and dietary treatments included 3 different lipid sources (soybean oil, duck fat, and palm oil), which were evaluated in corn-soybean meal diets (3250 kcal/kg metabolizable energy and 16.5% crude protein for grower diet and 3350 kcal/kg metabolizable energy and 15.5% crude protein for finisher diet). During days 15 to 28, feeding soybean oil and palm oil diets increased (P < 0.05) body weight gain (BWG), but decreased (P < 0.05) feed intake, feed-to-gain ratio (F/G) and caloric conversion compared with duck fat. During days 29 to 42, birds fed duck fat diet had higher BWG, but lower (P < 0.05) F/G and caloric conversion than those fed soybean oil and palm oil diets. Overall, feeding soybean oil diet increased (P < 0.05) BWG and final BW, but decreased (P < 0.05) F/G compared with palm oil. Birds fed duck fat diet had higher (P < 0.05) skin, subcutaneous fat and abdominal fat yield compared with palm oil. Left breast meat yield in soybean oil group was higher (P < 0.05) than that in duck fat and palm oil groups. Birds in soybean oil group had lower (P < 0.05) roasting loss, but higher (P < 0.05) comprehensive score compared with duck fat and palm oil. In summary, birds fed soybean oil diet had the best growth performance and taste scores for roasting, whereas the duck fat was better in abdominal fat and subcutaneous fat yield than soybean oil and palm oil in Pekin ducks from 15 to 42 d of age under the same nutritional level.

Percentage of Carcass and Internal Organs of Japanese Quail After Given Organic Feed

Japanese quail (Coturnix coturnix japonica) is one of the livestock sectors that is efficient in providing nutrition, both from eggs and meat. This study aims to examine the effect of provision of standard organic feed and organic feed containing cassava leaves, turmeric powder and ginger on increasing the percentage of carcasses and internal organs Japanese quail (Coturnix coturnix japonica). This study used an experimental method with a completely randomized design pattern. The test animals used were 30 females Japanese quail which were divided into 3 groups, i.e. P0: control (given commercial feed); P1: given standard organic feed (corn, soybeans, bran and fish meal); P2: given organic feed (corn, soybeans, bran, fish meal, cassava leaves, turmeric and ginger powder). Each group was treated with 5 replications. Data obtained were analyzed using analysis of variance (ANOVA) and continued with Duncan test with a confidence of 95% (P&gt;0.05). The results showed that the provision of organic feed could not increase the percentage of carcass, heart and liver, but could increase the percentage of Japanese quail gizzard (Coturnix coturnix japonica).

Future Grain Consumption Trends and Implications on Grain Security in China

Associated with population and income growth, grain consumption in China is expected to increase, and thus has inevitably influenced the food security. Using statistical data of the Food and Agricultural Organization of the United Nations (FAO) from 1978 to 2017, this study adopting the vector auto-regression (VAR) model and implied demand approach, projected the future consumption of major grains (rice, wheat, maize, and soybean) for food, feed, and other uses in China during 2018–2030. On this basis, it further discussed their implications on grain security. The results showed that during 2018–2030, the Chinese dietary structure would continue to shift from food grain to animal foods. As a result, the grain’s food consumption will decrease slightly (1.5%), while the feed consumption will increase significantly (31.4%), contributing 71.4% to the total increase of grain consumption. By 2030, the total grain consumption will increase by 20.2% to 846.2 million tons, of which 50.2% will be consumed for feeding animals. In the total consumption, maize will be the largest consumed grain variety, accounting for 39.2%. The security of rice and wheat would be optimistic in the future, while the security of maize and soybeans is likely to decline, and thus needs to be given high priority. These findings have great policy implications for improving the grain security, suggesting that in addition to promote the expansion of maize and soybean growing area by adjusting the cropping structure of the arable land, great efforts should be paid to improve the yield of both crops. In addition, residents should be guided to adjust the dietary structure, and also, it is important to improve the animal feeding efficiency.

Total replacement of the soybean meal with extruded semi-whole soybean in the piglets feeding during the nursery phase

The objective of this study was to evaluate the total replacement of soybean meal (SM) with extruded semi-whole soybean (ESWS) and its effects on feed digestibility, growth performance, plasma urea concentration (PUC) and economic feasibility. In Experiment I, a digestibility assay was undertaken using 12 crossbred pigs with an initial average body weight (IABW) of 18.85 ± 0.69 kg, distributed in a completely randomized design (CRD), with two treatments (reference diet and replacement of 30% of the reference diet by ESWS), six replicates and one pig per experimental unit. Experiment II involved 64 crossbred piglets, entire males, with an IABW of 7.46 ± 0.55 kg, distributed in a CRD, with two treatments (a control diet containing SM and another in which ESWS replaced SM), eight replicates and four animals per experimental unit. The digestible contents of dry matter, crude protein and gross energy of ESWS were 91.26%, 42.21% and 4,629 kcal kg -1, and the digestibility coefficients were 92.33%, 96.49% and 91.21%, respectively. Experiment II data indicated an effect on the feed conversion (FC) in the total period, in which pigs fed SM presented better FC. The diets affected the PUC in the pre-starter II phase and the total period, with pigs fed SM showing higher PUC. The cost of feed per kilogram of live weight gain increased in the starter phase. ESWS does not change the DWG and ADFI of piglets but worsens both the FC in the total period and economic results.

Mass transfer and kinetic study on BEA zeolite-catalysed oil hydroesterification

In the present study, we have assessed the effect of mass transfer on the both steps of the zeolite catalysed oil hydroesterification to produce biodiesel (hydrolysis of the feed soybean oil producing free fatty acid and free fatty acid esterification). Both reactions were conducted over BEA zeolite in a batch reactor. Experimental results indicate the absence of internal and external mass transfer limitations on the overall rate of either reaction. A reversible, second order reaction was invoked in order to estimate the reaction rate constant, pre-exponential factor and activation energy of the esterification and hydrolysis reactions. Activation energy of 25.9 kJ/mol (soybean oil hydrolysis), 38.5 kJ/mol (ethyl acetate hydrolysis), 24.8 kJ/mol (oleic acid esterification) and 46.7 kJ/mol (acetic acid esterification) were calculated over H-BEA zeolite, illustrating the importance of reactant size and the fact that reactions take place on the pour mouth of the mesopouros zeolite in a case of large molecules reaction. Experimental results suggested that the relative hydrophobicity of the catalyst is a key parameter, which influences whether the reaction is under kinetic or diffusion control.

Replacement of grains with soybean hulls ameliorates SARA-induced impairment of the colonic epithelium barrier function of goats

BackgroundThe effect of soybean hull feeding on the disruption of colonic epithelium barrier function was investigated in goats fed a high-concentrate diet. Twenty-one Boer goats (live weight, 32.57 ± 2.26 kg; age, 1 year) were randomly divided into three groups: low-concentrate diet (LC), high-concentrate diet (HC), and high-concentrate diet with soybean hulls (SH).ResultsWe found that the rumen fluid in the LC and SH group shown a higher pH value compared with the HC group. The mRNA and protein expression levels of extracellular regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (MAPK) in the colonic epithelium were significantly decreased in the SH group than in the HC group. Moreover, in goats fed the HC diet, SH treatment promoted gene expression and protein abundance of claudin-1, claudin-4, occludin, and ZO-1 in the colonic epithelium. Additionally, the injury to the colonic epithelium barrier caused by the HC diet was reversed by SH treatment.ConclusionsOur results indicated that supplemental SH feeding reverses the damage to colonic epithelium tight junctions by inhibiting the MAPK signalling pathway and has a protective effect on the colonic epithelium during SARA.

Changes in Ki-67 and Bcl-2 Expression in Rat Gastric Mucosa Secondary to Soybean Feeding: An Assessment of Proliferative and Apoptotic Activity of Flavonoids.

Background: Gastric cancer is a common malignancy and nutritional factors are believed to play an important role in its development. Isoflavones in soybean products are an important nutritional source, especially in some Asian Countries, having protective role against development of gastric cancer.In our study, we have prepared soybean extracts via different methodologies and assessed their apoptotic role and proliferation index in gastric mucosa of the rodents.Methodology: We have fed 2 months old 64 rats for a period of 1 month with soybean extracts prepared by 3different methodologies and in 6 different concentrations. Subsequently, the rodents were subjected to euthanasia and their gastric mucosal linings were processed accordingly for H &amp; E, Bcl-2, and Ki 67 staining. Thereafter, the specimens were evaluated under light microscopy.Results: In all the experimental groups fed with soybean extracts, weak staining was observed with Bcl-2, moresignificantly in the group of rodents fed with 100 mg/kg and 200 mg/kg ethanol extract as well as in the group fed with high ethyl acetate extract (p&lt;0.05). There was no difference between the groups with respect to Ki 67 staining and inflammation rate (p&gt;0.05).Conclusions: We have discovered that soybean extracts and specifically genistin and daidzein induces apoptosis at high concentrations. This observation makes us believe that soybeans have a protective role in the development of gastric cancer.

Influence of feeding thermally peroxidized soybean oil on growth performance, digestibility, and gut integrity in finishing pigs.

Consumption of peroxidized lipids has been shown to reduce pig performance and energy and lipid digestibility. Objectives of the current study were to evaluate the effect of feeding soybean oil (SO) with different levels of peroxidation on growth performance, lipid, N, and GE digestibility, plasma Trp, and gut integrity in finishing pigs. Fifty-six barrows (46.7 ± 5.1 kg initial BW) were randomly assigned to one of four diets in each of two dietary phases, containing either 10% fresh SO (22.5 °C) or thermally processed SO (45 °C for 288 h, 90 °C for 72 h, or 180 °C for 6 h), each infused with of 15 L/min of air. Peroxide values were 2.0, 17.4, 123.6, and 19.4 mEq/kg; 2,4-decadienal values were 2.07, 1.90, 912.15, and 915.49 mg/kg; and 4-hydroxynonenal concentrations were 0.66, 1.49, 170.48, and 82.80 mg/kg, for the 22.5, 45, 90, and 180 °C processed SO, respectively. Pigs were individually housed and fed ad libitum for 81 d to measure growth performance, including a metabolism period to collect urine and feces for determination of GE, lipid, N digestibility, and N retention. Following the last day of fecal and urine collection when pigs were in the metabolism crates, lactulose and mannitol were fed and subsequently measured in the urine to evaluate gut permeability, while markers of oxidative stress were evaluated in plasma, urine, and liver. There were no differences observed in ADFI (P = 0.91), but average daily gain (ADG) and gain:feed G:F were decreased in pigs fed 90 °C SO diet (P ≤ 0.07) compared to pigs fed the other SO diets. Pigs fed the 90 and 180 °C SO had the lowest (P = 0.05) DE as a % of GE compared to pigs fed the 22.5 °C SO, with pigs fed the 45 °C SO being intermediate. Lipid digestibility was similarly affected (P = 0.01) as energy digestibility, but ME as a % of DE was not affected by dietary treatment (P = 0.16). There were no effects of lipid peroxidation on N digested, N retained, or the urinary lactulose:mannitol ratio (P ≥ 0.25). Pigs fed the SO processed at 90 and 180 °1C had lower concentrations (P < 0.01) of plasma Trp compared to pigs fed the 22.5 and 45 °C SO treatments. Pigs fed 90 °C SO had the greatest (P < 0.01) concentrations of F2-isoprostane in plasma and urine thiobarbituric acid reactive substances compared to the other SO treatments. These results indicate that the change in FA composition and/or the presence of lipid peroxidation products in peroxidized SO may reduce ADG, G:F, and digestibility of GE and ether extract, but has little impact on N digestibility and balance or on gut permeability.

Influence of feeding thermally peroxidized soybean oil to finishing barrows on processing characteristics and shelf life of commercially manufactured bacon.

Objectives were to evaluate effects of feeding soybean oil (SO) with varying levels of peroxidation on fresh belly characteristics, processing yields, and shelf life of commercially manufactured bacon stored under food-service-style conditions. Fifty-six barrows were randomly assigned to 1 of 4 diets containing 10% fresh SO (22.5 °C) or thermally processed SO (45 °C for 288 h, 90 °C for 72 h, or 180 °C for 6 h), each infused with air at a rate of 15 L/min. Individually housed pigs were provided ad libitum access to feed for 81 d. On day 82, pigs were slaughtered, and on day 83, carcasses were fabricated and bellies collected for recording of weight, dimensions, and flop distance. Belly adipose tissue cores were collected for the analysis of iodine value (IV) by near-infrared spectroscopy (NIR-IV). Bacon was manufactured at a commercial processing facility, and sliced bacon was subsequently transferred to food-service-style packaging and subjected to 0-, 30-, 60-, or 90-d storage at -20 °C. Stored bacon was evaluated for thiobarbituric acid reactive substances (TBARS) and trained sensory evaluation of oxidized odor and flavor. Fresh belly and bacon processing traits were analyzed as a 1-way ANOVA with the fixed effect of SO, whereas shelf life traits were analyzed as a 1-way ANOVA repeated in time. There was no effect (P ≥ 0.30) of SO on belly weight, length, width, or thickness, but bellies of pigs fed 90 °C SO had greater (P ≤ 0.04) flop distance (more firm) than all other SO treatments. Belly fat NIR-IV of pigs fed 90 °C SO were 10.22 units less (P < 0.0001) than pigs fed 180 °C SO, which were 2.99 and 3.29 units less than belly adipose tissue of pigs fed 22.5 and 45 °C SO, respectively. There was no effect of SO on brine uptake or cooking yield of commercially manufactured bacon. There was a trend (P = 0.09) for bacon manufactured from bellies of pigs fed 45 and 90 °C SO to have greater slicing yields than those from pigs fed 22.5 and 180 °C SO. There were no SO × storage time interactions (P ≥ 0.27) for any shelf life trait. There was no difference in TBARS, oxidized odor, or oxidized flavor among the 4 SO treatments, although all 3 shelf life metrics increased (P < 0.0001) with storage time. Overall, feeding SO thermally processed at 90 and 180 °C reduced belly adipose tissue IV, but feeding peroxidized SO did not affect processing yields or shelf life characteristics of commercially manufactured bacon.

Influence of feeding thermally peroxidized soybean oil to finishing pigs on carcass characteristics, loin quality, and shelf life of loin chops.

The objective of this study was to evaluate the effect of feeding soybean oil (SO) with varying levels of peroxidation on carcass traits and shelf life of loins. Fifty-six barrows were randomly assigned to 1 of 4 diets containing 10% fresh SO (22.5 °C) or thermally processed SO (45 °C for 288 h, 90 °C for 72 h, or 180 °C for 6 h), each infused with air at a rate of 15 liter/min. Individually housed pigs were provided ad libitum access to feed for 81 d. At 82 d, pigs were slaughtered and hot carcass weight and liver weights were recorded. Carcass characteristics and fresh loin quality were evaluated 1 d postmortem. Loin chops from each carcass were overwrap-packaged and subjected to a 10-d simulated retail display. Daily measurements of L*, a*, b*, reflectance, and visual discoloration were conducted, evaluation of cooking loss and Warner-Bratzler shear force (WBSF) was conducted on chops stored 0, 5, and 10 d, and thiobarbituric acid reactive substances (TBARS) were evaluated on chops stored 0 and 10 d. Shelf life-related data were analyzed as a completely randomized design with repeated measures in time, with storage location (shelf) as a random effect. Carcasses of 90 °C pigs weighed 6.0, 8.6, and 6.9 kg less (P < 0.03) than 22.5 °C, 45 °C, and 180 °C carcasses, respectively. Livers of 90 °C and 180 °C pigs were 14.3% and 11.7%, respectively, heavier (P ≤ 0.02) than those from pigs fed 22.5 °C SO, with livers of 45 °C being intermediate. Livers of 90 °C pigs represented 0.12 percentage units less (P = 0.02) of ending live weight than livers of 180 °C pigs, and 180 °C livers were 0.12 percentage units less (P < 0.01) of ending live weight than those from pigs fed 22.5 °C SO, with 45 °C being intermediate. There was no difference (P ≥ 0.19) in back fat depth, loin muscle area, or estimated carcass lean percentage among SO treatments, nor was there an effect (P ≥ 0.13) of SO on any early post-mortem loin quality traits or loin composition. There was no effect (P > 0.14) of SO on cooking loss, WBSF, L*, a*, b*, hue angle, reflectance, discoloration, or TBARS; however, there was a tendency (P = 0.09) for chops of 45 °C pigs to have greater (P < 0.04) chroma than either 22.5 °C or 180 °C, with 90 °C being intermediate. Overall, feeding SO cooked at 90 °C for 72 h resulted in reduced carcass weight and dressing percentage; however, there was no evidence that feeding peroxidized SO was detrimental to shelf life of loin chops.

Effects of Soybean Based Supplements on Lamb Growth Performance and Muscle Fatty Acid Composition of Pasture Reared Lambs Finished on a Hgih Forage Diet

The effects of fat (F), protein (PR), and fat enriched protein (FP) soybean-based supplements on growth performance and muscle fatty acid profile of lambs were investigated. Thirty-three lambs (19.71 ± 1.74 kg) were randomly assigned to receive one of three supplements for the final 29 days of an 81-day stay on mixed pastures and then for an additional 39 days finishing period on drylots. During this latter period, lambs were fed orchard grass and alfalfa hay (1:1) along with the supplement. At the end of the study overall ADG, HCW and the fatty acid profile of lamb muscle were determined for 3 lambs in each treatment group. The quantity and quality of forage available to lambs while on pasture and accessibility to that forage ere similar across treatments. The average quantity of supplement and hay consumed daily was above 80% of what was offered and was similar across treatments. Daily gains of lambs after 29 days supplementation while on pasture differed (F=0.30, PR=0.22, FP=0.27 ± 0.02 kg/d, P=0.05). During the first 2 weeks in the drylot, ADG differed amongst treatments (F=-0.05, PR=0.23, FP=0.11 ± 0.05 kg/d, P 0.05), but dressing percentage tended to be higher in PR than in F and FP wethers (46.62, 44.27 and 43.85 ± 0.76%, P=0.08). The SFA: UFA of lamb muscle was lower in the F and FP than in PR treatments (0.98 vs. 1.38 and 1.22 ± 0.07, P=0.02). Feeding of soybean based non-commercial fat supplements achieved targeted increases in average daily gains in high forage fed lambs and influenced fatty acid profile of lamb muscle.

Blood Lipid Profile of Coturnix coturnix japonica Fed whith Organic Feed and Supplement Curcuma longa

&lt;p class="IsiAbstrakIndo"&gt;&lt;span lang="EN-GB"&gt;People’s concern about quail egg consumption has triggered the efforts to produce quail eggs with low cholesterol content by using organic feed. Organic feed is one type of feed rations made of mixing organic material. In this study, standard organic feed (rice bran, yellow corn, soybean, and fishmeal) and organic feed containing mackerel, cassava leaves, and turmeric powder was administere to female japanese quail. Organic feed containing mackerel, cassava leaves, and turmeric powder can affect the Japanese quail (&lt;/span&gt;&lt;em&gt;&lt;span lang="EN-GB"&gt;Coturnix coturnix japonica L.&lt;/span&gt;&lt;/em&gt;&lt;span lang="EN-GB"&gt;) lipid profile. This research used experimental methods. Twenty-seven female Japanese quails were divided into 3 treatment groups (PO: commercial feed, P1: standard organic feed, P2: feed containing organic turmeric powder, cassava leaves, and mackerel) for 145 days with 9 repeat. Data obtained were in the form of average daily feed intake and blood lipid profiles of each treatment group. Further data analysis was performed by analysis of variance (ANOVA) followed by Duncan test. The results showed that administration of organic feed containing mackerel, cassava leaves, and turmeric powder can reduce the levels of cholesterol, triglycerides, and LDL but increase the HDL levels in Japanese quail. Supplementation of turmeric powder improve the chemical quality of Japanese quail eggs, so it is good for the development of quail embryos as well as for consumption. It can be concluded that organic feed and turmeric powder supplementation stabilize blood lipid profile and improve the reproduction of Japanese quail egg low cholesterol, so good for the development of embryo quail and for consumption.&lt;/span&gt;&lt;/p&gt;

Land-use change emissions from soybean feed embodied in Brazilian pork and poultry meat

Fodder for feeding pork and poultry requires a large amount of cultivated and processed agricultural crops, which are often related to greenhouse gas emissions from land-use change (LUC). Given the rapid growth in meat production in Brazil for domestic consumption and international export, the link between LUC emissions and consumption of pork and poultry meat is central to evaluating the role of Brazilian meat production and the implications for international climate policies. In this paper, we first estimate LUC emissions from soybean production destined to feed pork and poultry in Brazil during the period 2008–2012. Second, we allocate such emissions to domestic and international consumption of pork and poultry meat. Concerning international consumption, Brazilian export of meat to 189 countries is evaluated. Although the majority of the LUC emissions from soybean production are tied to the domestic consumption, 17% and 39% of the emissions embodied in Brazilian pork and poultry, respectively, are exported to other countries. The most prominent destinations of Brazilian pork and poultry in terms of embodied LUC emissions are Eastern Europe, Asia and South America. In the case of pork, the fluxes of emissions from Brazil to Ukraine (1.28 Mt CO2-eq) and Russia (1.18 Mt CO2-eq) are particularly large. In the case of poultry, the largest transfers of embodied emissions are from Brazil to developing countries and especially the Middle East. These results are relevant to environmental policies and international agreements aimed at achieving the sustainability of Brazil's meat sector.

Effect of feeding soybean meal and differently processed peas on intestinal morphology and functional glucose transport in the small intestine of broilers

Peas are locally grown legumes being rich in protein and starch. However, the broad usage of peas as a feed component in poultry nutrition is limited to anti-nutritional factors, which might impair gut morphology and function. This study investigated the effect of feeding raw or differently processed peas compared with feeding a soybean meal-based control diet (C) on intestinal morphology and nutrient transport in broilers. A total of 360 day-old broiler chicks were fed with one of the following diets: The C diet, and 3 diets containing raw peas (RP), fermented peas (FP) and enzymatically pre-digested peas (EP), each supplying 30% of dietary crude protein. After 35 d, jejunal samples of broilers were taken for analyzing histomorphological parameters, active glucose transport in Ussing chambers and the expression of genes related to glucose absorption, intestinal permeability and cell maturation. Villus length (P = 0.017) and crypt depth (P = 0.009) of EP-fed broilers were shorter compared to birds received C. The villus surface area was larger in broilers fed C compared to those fed with the pea-containing feed (P = 0.005). Glucose transport was higher for broilers fed C in comparison to birds fed with the EP diet (P = 0.044). The sodium-dependent glucose co-transporter 1 (SGLT-1) expression was down-regulated in RP (P = 0.028) and FP (P = 0.015) fed broilers. Correlation analyses show that jejunal villus length negatively correlates with the previously published number of jejunal intraepithelial T cells (P = 0.014) and that jejunal glucose transport was negatively correlated with the occurrence of jejunal intraepithelial leukocytes (P = 0.041). To conclude, the feeding of raw and processed pea containing diets compared to a soybean based diet reduced the jejunal mucosal surface area of broilers, which on average was accompanied by lower glucose transport capacities. These morphological and functional alterations were associated with observed mucosal immune reactions. Further studies are required elucidating the specific components in peas provoking such effects and whether these effects have a beneficial or detrimental impact on gut function and animal health.

Toxicologic evaluation of chronic feeding of glyphosate-resistant transgenic soybean GTS40-3-2 meal to rats

To assess the safety of glyphosate-resistant transgenic soybean, the transgenic soybean GTS40-3-2 meal and its non-transgenic counterpart parent A5403 were fed to first- and second-generation Sprague-Dawley (SD) rats (Rattus norvegicus) for 195 days. The soybean meal made up 20% of the total weight of the feed. Body weight, food consumption, hematology, blood biochemistry, and pathological observations were monitored weekly. The results showed that rats of each group experienced good growth and development, and although results of some organ weight ratios and serum biochemistry between the groups were significantly different (P&lt;0.05), they were of no biological significance because the variations were within the normal range. These results indicate that glyphosate-resistant transgenic soybean GTS40-3-2 meal is as safe as its non-transgenic parent soybean A5403 in a long-term SD rat feeding study. The study provides experimental evidence for the safe usage of transgenic soybean GTS40-3-2.

Influence of immunostimulant polysaccharides, nucleic acids, and Bacillus strains on the innate immune and acute stress response in turbots (Scophthalmus maximus) fed soy bean- and wheat-based diets.

Immunostimulants are widely applied in aquaculture practice and may have beneficial effects on the immune system and physical functions allowing higher tolerance to stress. In the current study, the impact of four (i-iv) dietary active ingredients on the immune and stress response of turbot was examined in two experiments (I and II). A basal low fish meal (FM; 32%) diet was formulated and supplemented with (i) yeast β-glucan and mannan oligosaccharide (GM), (ii) alginic acid (AC), (iii) yeast nucleotides and RNA (NR), or (iv) Bacillus strains (BS). The basal diet (C-LF) and a high FM (59%) control (C-HF) were maintained. All six diets were fed to juvenile turbots for 84days in experiment I and for additional 28days prior to experiment II. Immunological and hematological parameters were determined in experiment I. In experiment II, physical stress response to a typical short-term (<1day) aquaculture handling procedure (combination of capture, netting/transfer, and crowding) was investigated. For this, turbot blood was sampled before and at 0.5, 1, 4, and 24h post stress. Plasma lysozyme activity, neutrophil reactive oxygen species (ROS) production, and total plasma protein levels did not significantly differ between treatment groups; however, plasma cholesterol increased significantly in fish fed GM, AC, NR, and C-HF compared to C-LF (I). A significant increase in plasma glucose and triglyceride was observed in GM and NR treatments, while glucose levels were significantly higher in C-HF compared to C-LF. Moreover, the immunostimulant-supplemented diets exhibited significantly lower cortisol levels compared to controls C-LF (at 0.5h) and C-HF (at 1h) post stress, respectively (II). According to our findings, FM substitution did not modulate the innate immune response but was associated with reduced levels of cholesterol. Dietary immunostimulants were not effective enough to boost the immune response, but we believe they might be helpful to trigger metabolic advantages during stressful handling events on fish farms.

Efficacy of the Integrated Harrington Seed Destructor on Weeds of Soybean and Rice Production Systems in the Southern United States

Herbicide‐resistant weeds affect every major cropping system today. Worldwide, there are 47 and 51 confirmed cases of herbicide‐resistant weed populations in soybean [Glycine max (L.) Merr.] and rice (Oryza sativa L.) production systems, respectively. Alternatives to herbicides are necessary to help combat herbicide‐resistant weeds. The integrated Harrington Seed Destructor (iHSD) has been developed to destroy weed seeds during crop harvest but has yet to be tested on weeds in soybean or rice. Thus, the objective of this research was to determine the effectiveness of the iHSD in soybean and rice and to evaluate any limitations. Three experiments were conducted using a stationary iHSD mill. First, the efficacy of the iHSD was evaluated on weed seeds incorporated into soybean residue or rice chaff. Second, varying soybean chaff feeding rates were tested to determine the amount that could be processed without interfering with weed seed destruction. Third, varying soybean chaff moisture levels were tested to determine any limitations that high moisture content may have on the iHSD. The iHSD demonstrated high weed seed destruction efficacy (&lt;1% survival) for 11 of the 12 weed species of soybean. Common cocklebur (Xanthium strumarium L.) seed had 3% survival. In rice, &lt;1% survival was observed in all weed species. The soybean feeding rate and moisture experiments yielded &lt;1% survival across all treatments. These results show that the use of the iHSD can be highly effective in soybean and rice production for reducing inputs to the soil seedbank. These studies highlight the promising seed destruction potential of the iHSD but also demonstrate opportunities for further research evaluating the iHSD as a combine‐fitted system operating under commercial scale production fields.

Effect of feeding soybean meal and differently processed peas on the gut mucosal immune system of broilers

Peas are traditionally used as a protein source for poultry. However, peas contain antinutritional factors (ANF), which are associated with the initiation of local and systemic immune reactions. The current study examined the effect of feeding raw or differently processed peas in comparison with feeding a soybean meal (SBM) based control diet (C) on the gut mucosal immune system of broilers in a 35 day feeding trial. In six replicates, a total of 360 one-day-old male broilers were randomly allocated to four different groups receiving C, or three treatment diets containing raw, fermented, and enzymatically pre-digested peas, each supplying 30% of required crude protein. After slaughtering, jejunal samples were taken for immunohistochemical, flow cytometric, and gene expression analyses. Investigations were focused on the topological distribution of intraepithelial leukocytes (villus tip, villus mid, and crypt region) as well as on the further characterization of the different intraepithelial lymphocytes (IEL) and concomitant pro- and anti-inflammatory cytokines. Broilers receiving the raw or processed pea diets had higher numbers of intraepithelial CD45+ leukocytes in the tip (P = 0.004) and mid region (P < 0.001) of villi than birds fed C. Higher numbers of intraepithelial CD3+ lymphocytes were found in the villus tip (P = 0.002) and mid region (P = 0.003) of birds fed raw or processed pea containing diets in comparison with those fed C. The flow cytometric phenotyping showed a similar relative distribution of IEL among the feeding groups. The expression of intestinal pro- and anti-inflammatory cytokines was affected by feeding the different diets only to a minor extent. To conclude, feeding of diets formulated with raw and processed peas in comparison with feeding a SBM control diet initiated mucosal immune responses in the jejunum of broilers indicated by a quantitative increase of intraepithelial T cells. Further research is needed in order to ascertain the specific factors which are responsible for observed local immune reactions and how these local reactions might affect the immune status and health of broilers.