Fecal Nitrogen Output Research Articles

PSII-13 Effects of benzoic acid, bromelain, adipic acid, and humic substance supplementation on nitrogen balance, urine pH, slurry pH, and manure odorous compounds in pigs

Abstract The objective of the present study was to evaluate the effects of benzoic acid, bromelain, adipic acid, and humic substance supplementation on nitrogen balance, urinary pH, slurry pH, and manure odorous compounds in pigs. Fifteen barrows with an initial body weight of 37.9 kg (standard deviation = 4.1) were individually housed in metabolism crates. The animals were allocated to a triplicated 5 × 2 incomplete Latin square design with 15 animals, 5 experimental diets, and 2 periods. The basal diet mainly consisted of corn, soybean meal, and rapeseed meal. Four experimental diets were prepared by supplementing each additive at a concentration of 10 g/kg at the expense of corn starch to the basal diet. Each period consisted of a 4-d adaptation period and a 24-h collection period for slurry, followed by a 4-d collection period for feces and urine. On d 5, feces and urine were collected for 24 h, and the feces and urine were mixed in a ratio of fecal weight to urine weight at the end of the collection to obtain slurry samples. The fecal nitrogen (N) output was greatest (P < 0.05) in pigs fed the diet supplemented with humic substances. The apparent total tract digestibility of N in pigs fed the humic substance diet was less (P < 0.05) than other groups. The urinary pH in pigs fed the adipic acid diet was less (P < 0.05) than that in other groups except the benzoic acid group. The slurry pH tended to differ among the treatment groups (P = 0.074) with the least value in pigs fed the adipic acid diet. The concentrations of indole in slurry (P = 0.084) and isovalerate in feces (P = 0.062) tended to differ among the groups with the least values in the pigs fed the humic substance diet. In conclusion, benzoic acid supplemented has the potential to increase N utilization in pigs, and adipic acid supplemented to pig diets can decrease urinary pH and slurry pH. Although benzoic acid and adipic acid have limited effects in reducing odorous compounds, humic substances have potential to reduce some odorous compounds.

54 Impact of Ileal Indigestible Protein on Nitrogen Excretion and Fecal Microbiota may be Greater Compared with Total Protein Concentration of Diets in Growing Pigs

Abstract The effect of changes in dietary ileal indigestible protein concentration may be more influential in the amount of nitrogen flowing to the large intestine compared with changes in total dietary protein concentration. For this reason, we hypothesized that an increase in dietary ileal indigestible protein concentration induces an increase in hindgut nitrogen utilization and nitrogen excretion and a shift in fecal microbiota in growing pigs, when compared with pigs given a high total protein diet. Three diets were prepared: 1) standard protein diet based on corn and soybean meal, 2) high-indigestible protein diet in which autoclaved soybean meal replaced normal soybean meal in the first diet, and 3) high protein diet where the inclusion rate of soybean meal was greater than that of the other diets. T-cannulated barrows (n = 18; initial BW = 63.4 ± 8.0 kg) were allotted to the 3 diets in a randomized complete block design with BW as a blocking factor. Pigs were individually housed in pens and the experiment lasted for 23 days. On d 7 and 21, fecal samples were collected by rectal massage for microbiota analysis. Grab samples of feces were collected on d 20 and 21, and ileal digesta were collected on d 22 and 23 for the determination of energy and nitrogen utilization. Energy and nitrogen utilization data were analyzed using the MIXED procedure of SAS using a pig as the experimental unit and a model that included diet as a fixed variable and block as a random variable. For microbiome analysis, R was used. Alpha and beta diversity metrics were analyzed and the difference in abundance of the genus was determined using the DESeq2 function. The high-indigestible protein diet achieved decreased apparent ileal digestibility of nitrogen and resulted in greater ileal indigestible nitrogen concentration (P < 0.05). Apparent total tract digestibility of nitrogen was less (P < 0.05), and correspondingly fecal nitrogen concentration and daily fecal nitrogen output were greater (P < 0.05) in the high-indigestible protein diet. Apparent post-ileal digestibility and hindgut disappearance of nitrogen and gross energy were the greatest (P < 0.05) in the high protein diet. Beta diversity metrics of feces in the high-indigestible protein diet on d 21 were different (q < 0.05) from those in the other two diets, which indicates a shift in fecal microbiota. According to the DESeq2 results, the direction of the microbiota shift induced by the high-indigestible protein diet may have reduced hindgut fiber utilization. In conclusion, an increase in dietary ileal indigestible protein increased nitrogen excretion and shifted fecal microbial communities but did not increase hindgut nitrogen utilization.

Nutrient digestibility and nitrogen balance in different pig breeds fed raw, sprouted, or roasted (Vigna unguiculata) diets

The study examined effects of feeding iso-nutrient (150 g CP, 17.3 MJ ME kg−1) raw (RCP), sprouted (SPC), or roasted (RSCP) cowpea diets to Windsnyer (W), Large White (LW) × Landrace (LR), and the 3-way crossbred (W × LW × LR) growing pigs. Diet dry matter (DM) digestibility was estimated using standard, 3-step (gastric, small intestines, colon) in vitro digestion. Dietary in vivo nutrient digestibility and nitrogen balance were evaluated using 3 weaned pigs of each genotype. Pigs were housed in individual metabolic cages. The diets were assigned to pigs in a 3 × 3 change-over factorial experiment within three balanced, 3 × 3 Latin squares. Feeding periods consisted of 7 days adaption + 5 days’ measurement of feed intake, and the total faecal and urine excretions. The SPC diet increased step 3 in vitro DM digestibility compared to RSCP (P < 0.05). Metabolic size-scaled feed consumption was higher on the RCP compared to the RSCP diet (P < 0.05). Cowpea processing reduced apparent DM and ash digestibility (P < 0.05). LW × LR pigs exhibited lower digestibility of ash and acid detergent fiber (ADF) compared to 3-way crossbred pigs (P < 0.05). Significant genotype-diet interactions were observed for nitrogen intake (P < 0.0001), digestible nitrogen (P = 0.043), urinary nitrogen output (P < 0.0001), faecal nitrogen output (P < 0.0001), total nitrogen excretion (P < 0.0001), and nitrogen retention (P < 0.001). The biological value of feed protein was higher for W pigs than 3-way crossbred pigs (P < 0.05). Genotype-diet interactions suggested unique digestive and, or metabolic adaptive traits in the utilization of the differently processed cowpeas, which need further investigation.

Impact of ileal indigestible protein on fecal nitrogen excretion and fecal microbiota may be greater compared with total protein concentration of diets in growing pigs.

In the current study, we hypothesized that an increase in dietary ileal indigestible protein concentration induces an increase in hindgut nitrogen utilization and nitrogen excretion and a shift in fecal microbiota in growing pigs, when compared to pigs given a high total protein diet. Three diets were prepared: 1) standard protein diet based on corn and soybean meal, 2) high-indigestible protein diet in which autoclaved, low-digestible soybean meal replaced soybean meal in the first diet, and 3) high protein diet where the inclusion rate of soybean meal was greater than that of the other diets. The 3 diets were fed to 18 barrows that were fitted with T-cannula at the ileo-cecal junction (initial body weight = 63.4 ± 8.0 kg) in a randomized complete block design with body weight as a blocking factor. Pigs were individually housed in pens and the experiment lasted for 23 d. On days 7 and 21, fecal samples were collected by rectal massage for microbiota analysis. Grab samples of feces were collected on days 20 and 21, and ileal digesta were collected on days 22 and 23 for the determination of energy and nitrogen utilization. Lower apparent ileal digestibility of nitrogen in the high-indigestible protein diet containing autoclaved soybean meal resulted in greater ileal indigestible nitrogen concentration (P < 0.05). Apparent total tract digestibility of nitrogen was lower (P < 0.05), and correspondingly nitrogen concentration and daily fecal nitrogen output were greater (P < 0.05) in the high-indigestible protein diet compared with the other diets. Apparent post-ileal digestibility and hindgut disappearance of nitrogen and gross energy were the greatest (P < 0.05) in the high protein diet, whereas a statistical difference was not observed in those variables between the standard protein diet and the high-indigestible protein diet. Beta diversity metrics of feces in the high-indigestible protein diet on day 21 were different (q < 0.05) from those in the other two diets, which indicates a shift in microbial communities. According to the results of the DESeq2, the direction of microbiota shift induced by the high-indigestible protein diet may have reduced fiber utilization in the hindgut. In conclusion, an increase in dietary ileal indigestible protein concentration increased fecal nitrogen excretion and shifted fecal microbial communities but did not increase nitrogen utilization in the hindgut.

Nitrogen output in the urban environment using a vegetarian canine diet.

Pet owners are increasingly concerned about the links between health status, animal welfare, environmental impacts, climate change and consumption of animal products. Accordingly, many owners are increasingly interested in vegetarian diets for themselves and their companion animals. However, such diets should be investigated nutritionally regards digestibility as well as on fecal quality and nitrogen output. In light of this trend, six Beagle dogs were included in a cross-over experimental design and offered a vegetarian diet containing wheat gluten (8.81%), rice protein (8.81%) and sunflower oil (6.84%) or an meat-based diet containing poultry meal (19.5%) and poultry fat (5.23%). The dogs received extruded complete diets for 12 days (adaptation and collection period, each 6 days). The dogs fed both diets showed a high and identical palatability (scoring of food intake) of the experimental diets. No significant differences occurred regarding digestibility of organic matter, crude protein and crude fat between vegetarian and meat-based diets. However, dogs fed the meat-based diet had higher (p < 0.05) nitrogen-free extract digestibility (89.5%) compared to those fed the vegetarian diet (88.6%). The amount of nitrogen excreted in feces (g)/kg BW0.75 was slightly, but not significantly, higher for dogs fed the vegetarian diet compared to those fed the meat-based diet (0.88 vs 0.79). The fecal consistency scores were considered to be within an acceptable range (well formed and firm). The mass of the feces between both groups were similar (62.9 g wet feces/100 g dry matter food) for vegetarian and meat-based diets. Additionally, the fecal dry matter content was comparable between both groups (29.0% and 29.6% for vegetarian and meat-based diets, respectively). In conclusion, the results of this study appear to indicate that virtually the only significant difference between the two diets was lower nitrogen-free extract digestibility in the vegetarian diet. However, the vegetarian diet did not result in a significant difference in amount of nitrogen excreted in feces.

Pectin Influences the Absorption and Metabolism of Polyphenols from Blackcurrant and Green Tea in Rats.

Consumption of polyphenols and dietary fiber as part of a normal diet is beneficial to human health. In this study, we examined whether different amounts of dietary soluble fiber (pectin) affect the absorption and metabolism of polyphenols from blackcurrant and green tea in rats. After 28 days, the rats fed blackcurrant and green tea with pectin (4 or 8%) had significantly lower body weight gain and food intake compared to the rats fed a control diet. Rats fed a blackcurrant and green tea diet with 8% pectin had significantly higher fecal nitrogen output and lower protein digestibility. No polyphenols were observed in the urine, feces and plasma of rats fed the control diet. Parent catechins and flavonols were absent in urine obtained from all diet groups. Gallocatechin glucuronide was only observed in the plasma of rats fed the blackcurrant and green tea diet without pectin. Meanwhile, epicatechin and catechin gallate were present in the feces of rats fed a blackcurrant and green tea diet with and without 4% pectin. Pectin (4 or 8%) added to the blackcurrant and green tea diet increased the plasma antioxidant capacity in rats. Inclusion of pectin in the diet altered the host absorption and metabolism of polyphenols from blackcurrant and green tea.

Effects of ractopamine hydrochloride on nutrient digestibility and nitrogen excretion of finishing beef cattle.

The objective was to quantify the effects of the beta-adrenergic agonist (β-AA) ractopamine hydrochloride (Actogain, Zoetis, Parsippany, NJ) on nitrogen excretion and nutrient digestibility in feedlot cattle. In experiment 1, 12 Simmental × Angus steers were blocked by bodyweight (531 ± 16 kg) and used in a randomized complete block design. Dietary treatments included: 1) a control without β-AA (CON) or 2) 400 mg/steer/d ractopamine hydrochloride (RAC) for 35 d before slaughter. Diets contained (DM basis) 55% dry-rolled corn, 20% corn silage, 15% modified wet distillers grains with soluble, and 10% supplement. For each block, total collection of feed, orts, feces, and urine were conducted for two 5 d sampling periods during week 2 and 4 of RAC supplementation. No interaction (P > 0.21) between treatment and collection period was observed for any parameter evaluated. Dietary treatment had no effect (P = 0.51) on DMI, but RAC had decreased fecal DM output (P = 0.04) compared with CON. Thus, RAC had greater apparent total tract DM digestibility (77.2 vs. 73.5%; P < 0.01), N digestibility (72.4 vs. 69.4%; P = 0.01), and NDF digestibility (65.6 vs. 60.2%; P < 0.01) than CON. Although treatment did not affect nitrogen intake (P = 0.52), RAC tended to reduce total nitrogen excretion (113.3 vs. 126.7 g/d; P = 0.10) compared with CON due to a tendency for decreased fecal nitrogen output (53.9 vs. 61.3 g/d; P = 0.10). However, dietary treatment had no effect (P = 0.53) on urinary nitrogen output or percentage of urinary nitrogen excreted as urea (P = 0.28). Experiment 2 was an in vitro experiment conducted to validate the effects of RAC on nutrient digestibility using Simmental × Angus heifers (451 ± 50 kg). Rumen fluid was collected individually by stomach tube from CON- (n = 9) and RAC-fed (n = 10) heifers to inoculate bottles containing a CON or RAC-containing substrate in a split–plot design. No interaction between rumen fluid source and in vitro substrate was observed. Greater IVDMD (P = 0.01) was observed in rumen fluid from RAC-fed heifers compared with rumen fluid from CON-fed heifers. The inclusion of RAC in the in vitro substrate increased IVDMD (P < 0.01). Overall, feeding RAC increased microbial digestion of the dry-rolled corn-based finishing diet to increase total tract dry mater digestion by 5% and reduce nitrogen excretion by 10.6% in the 35 d period prior to slaughter.

Nitrogen balance response to varying levels of dietary protein in slow-growing Windsnyer pigs

Protein requirements for slow-growing pigs have not been defined. The study was designed to determine the relationship between the level of dietary protein and nitrogen balance in slow-growing Windsnyer pigs. Thirty Windsnyer pigs with an initial bodyweight of 34.7 (SD = 0.85) kg were randomly assigned to six experimental diets containing 193, 174, 154, 135, 117, and 97 g/kg of crude protein (CP). Pigs were penned individually. There were five pigs in each diet. The faecal nitrogen output (NFO) showed a decreasing quadratic relationship with protein level. A linear relationship between protein level and urinary nitrogen excretion was observed (P =0.0001). The total nitrogen excreted showed a significant quadratic relationship to decreasing levels of CP in the diets (P =0.0001). The apparent digestibility of nitrogen had a linear relationship with dietary CP level (P =0.0005). A quadratic relationship between dietary protein level and N utilization was observed (P =0.0001). The optimum protein level for total nitrogen excreted, nitrogen utilization and biological value of feed protein were attained at 135 g/kg protein level. The efficiency of nitrogen utilization supports the low protein diet. The present results suggest reducing the dietary protein level for Windsnyer pigs to decrease amount of excreted N that is deposited into the environment.Protein requirements for slow-growing pigs have not been defined. The study was designed to determine the relationship between the level of dietary protein and nitrogen balance in slow-growing Windsnyer pigs. Thirty Windsnyer pigs with an initial bodyweight of 34.7 (SD = 0.85) kg were randomly assigned to six experimental diets containing 193, 174, 154, 135, 117, and 97 g/kg of crude protein (CP). Pigs were penned individually. There were five pigs in each diet. The faecal nitrogen output (NFO) showed a decreasing quadratic relationship with protein level. A linear relationship between protein level and urinary nitrogen excretion was observed (P =0.0001). The total nitrogen excreted showed a significant quadratic relationship to decreasing levels of CP in the diets (P =0.0001). The apparent digestibility of nitrogen had a linear relationship with dietary CP level (P =0.0005). A quadratic relationship between dietary protein level and N utilization was served (P =0.0001). The optimum protein level for total nitrogen excreted, nitrogen utilization and biological value of feed protein were attained at 135 g/kg protein level. The efficiency of nitrogen utilization supports the low protein diet. The present results suggest reducing the dietary protein level for Windsnyer pigs to decrease amount of excreted N that is deposited into the ironment.&#x0D; Keywords: apparent nitrogen digestibility, ideal protein level, nitrogen retention, excreted nitrogen, nitrogen utilization

Growth performance and nitrogen utilization by West African dwarf goats fed supplemented with varying ratios of and Panicum maximum Afzelia africana Newbouldia laevis

Afzelia africana and Newbouldia laevis have recognizable potential as important locally available forage resources but yet to be explored and exploited for ruminants in the tropics. This study was therefore investigated to determine the effect of supplementing varying ratios of Afzelia africana and Newbouldia laevis with Panicum maximum on growth performance and nitrogen utilization of West African dwarf goats. Twenty female West African dwarf goats, aged between 5 and 7monhs old with an average weight of 6.62 ± 0.41kg were randomly allotted to five dietary treatments with four goats per treatment in a completely randomized design. The treatment diets included T (100% Panicum maximum), T (25% Afzelia africana and 75% Panicum maximum), T (50% Afzelia africana and 50% Panicum maximum), T (25% Newbouldia laevis and 75% Panicum maximum) and T (50% Newbouldia laevis and 50% Panicum maximum). A metabolism trial was conducted at the end of the feeding trial to assess the effects of the diets on nitrogen utilization after the growth study on the goats. Results obtained showed that goats on diets T were significantly (P&lt;0.05) higher in terms of final live weight (9.95kg), metabolic weights (6.20LW ), total weight gain (3.15kg) and growth rate (35.50g.d ) at (P &lt; 0.01) while nitrogen intake (7.86g/d ), metabolic nitrogen intake (1.76g.kg LW ), nitrogen balance (6.81g.d ) were higher (P &lt; 0.001) than goats on other diets. Urinary nitrogen output (6.81g.d ) was significantly (P&lt;0.01) higher in goats on T compared with goats on other diets. No significant (P&gt; 0.05) effect in treatment diets with regards to initial and average live weights, faecal nitrogen output, and total nitrogen output with nitrogen retention. It was concluded that goats fed 50% Afzelia Africana and 50% Panicum maximum had better nitrogen utilization for growth performance.

Effects of a high-protein corn product compared with soy and canola protein sources on nutrient digestibility and production responses in mid-lactation dairy cows

An experiment was conducted to assess the effects of a novel and proprietary high-protein corn product [56% crude protein (CP)] relative to other common sources of protein on the lactation performance of dairy cows. Twenty-four Holstein cows (620 ± 47.7 kg of body weight, 111 ± 34 d in milk, 2.28 ± 0.46 lactations; mean ± standard deviation) were randomly assigned to treatment sequence in a replicated 4 × 4 Latin square design balanced for carryover effects. Cows were individually fed 1 of 4 diets with a different protein concentrate source during each 28-d period, including soybean meal (SBM), high-protein corn product (HPCP), soybean meal with rumen-bypass soy protein (SBMBP), and canola meal with rumen-bypass soy protein (CANBP). Diets were formulated for equal concentrations of CP and balanced to meet predicted lysine and methionine requirements. The SBM diet was formulated to provide 5.7% rumen-undegradable protein (RUP), whereas SBMBP and CANBP diets were formulated for 6.8% RUP to match HPCP. Data were analyzed using mixed models with the fixed effects of treatment, period, square, the interactions of treatment and period and of treatment and square, and the random effect of cow. The CANBP diet increased dry matter intake (DMI) compared with SBM and HPCP. Treatment affected milk yield, as SBMBP and CANBP increased yield compared with SBM, but HPCP decreased milk yield compared with all treatments. The HPCP diet reduced CP intake as a percent of total DMI and increased the CP content of orts, indicative of selection against HPCP. The HPCP diet also decreased apparent total-tract and CP digestibility, leading to less urine nitrogen excretion and greater fecal nitrogen output. The SBMBP and CANBP diets performed similarly in nearly every variable measured, except that SBMBP increased milk urea nitrogen. In conclusion, the HPCP diet reduced yield of milk and milk components, likely because of reduced apparent total-tract dry matter and CP digestibility.

Comparative energy and nitrogen excretion from growing pigs fed on corn, sorghum and wheat-based diets

Sorghum and wheat grains have been previously identified as potential substitutes for corn in diets for pigs based on the available energy. This experiment was conducted to compare growth performance and faecal energy and nitrogen output per weight gain in growing pigs fed on corn (CSBM), sorghum (SSBM) or wheat soybean meal based diets (WSBM). Sixty crossbred pigs (23.4 ± 1.6 kg of body weight) were allotted to 3 diets with 5 replicate pens per treatment according to sex and weight in a randomized complete block design. The CSBM included 703.4 g/kg of corn during day 1–35 (phase 1) and 736 g/kg during day 36–70 (phase 2). The corn content in CSBM was partially (500 g/kg; phase 1) or completely (phase 2) replaced by sorghum in SSBM or wheat in WSBM. No differences were detected in average daily gain (ADG) or average daily feed intake (ADFI) between CSBM and SSBM treatments. Pigs fed on WSBM had lower ADFI than those fed on CSBM or SSBM (P < 0.05). The ADG of pigs fed on WSBM tended to decrease during phase 1 (P = 0.09) and 2 (P = 0.06) and decreased overall (P < 0.05). Compared with SSBM, WSBM increased feed conversion efficiency and apparent total tract digestibility (ATTD) of dry matter and gross energy (P < 0.05). The ATTD of crude protein in CSBM was greater than that in SSBM and lower than that in WSBM (P < 0.05). Pigs fed on CSBM had lower faecal energy and nitrogen output per weight gain than those fed on SSBM and greater than those fed on WSBM (P < 0.05). Serum urea nitrogen concentration in WSBM was greater than that in CSBM and SSBM (P < 0.05). Collectively, sorghum could be used as an alternative energy source for corn in diets fed to growing pigs without negative effects on growth performance. However, given the feed conversion efficiency and manure emissions, wheat may be a superior energy ingredient in diets for growing pigs with the low nutrient loss and output in manure.

Association between residual feed intake, digestion, ingestive behavior, enteric methane emission and nitrogen metabolism in Nellore beef cattle.

This study aimed to evaluate nutrient intake and digestibility, enteric methane emission and nitrogen utilization efficiency in Nellore cattle ranked by residual feed intake (RFI). Twenty-four Nellore bulls at 466±24days of age and with 352±14.6kg of body weight, classified as low and high RFI, were evaluated. Animals were kept in individual pens for three periods of 28days and variables were measured. Data were analyzed as repeated measures over time, considering as fixed effects RFI class, period and RFI class x period interaction, and linear (co)variate of age. No significant differences in dry matter or nutrient intake were detected between RFI classes, but total digestible nutrients intake tended to be lower in low RFI animals, and apparent nutrient digestibility was higher in high RFI animals. Partial efficiency of growth tended to be lower in high RFI animals. RFI class did not interfere with enteric methane production or microbial protein synthesis, but fecal nitrogen output was higher in low RFI animals. The greater efficiency of low RFI animals is consequence of lower maintenance requirements, since energy from higher nutrients digestibility in high RFI animals was spent on metabolic processes other than body tissue deposition.

Effect of supplementation of allicin on methanogenesis and ruminal microbial flora in Dorper crossbred ewes.

BackgroundGarlic extracts have been reported to be effective in reducing methanogenesis. Related mechanisms are not well illustrated, however, and most studies have been conducted in vitro. This study investigates the effects of supplementary allicin (AL) in sheep diet on in vivo digestibility, rumen fermentation, and shifts of microbial flora.MethodsTwo experiments were conducted using Dorper × thin-tailed Han crossbred ewes. In experiment 1, eighteen ewes (60.0 ± 1.73 kg BW) were randomly assigned for 29 days to either of two dietary treatments: a basal diet or the basal diet supplemented with 2.0 g AL/head·day to investigate supplementary AL on nutrient digestibility and methane emissions. In experiment 2, six ewes (65.2 ± 2.0 kg BW) with ruminal canulas were assigned to the same two dietary treatments as in experiment 1 for 42 days to investigate supplementary AL on ruminal fermentation and microbial flora. The methane emissions were determined using an open-circuit respirometry system and microbial assessment was done by qPCR of 16S rRNA genes.ResultsSupplementary AL increased the apparent digestibility of organic matter (P < 0.001), nitrogen (P = 0.006), neutral detergent fiber (P < 0.001), and acid detergent fiber (P = 0.002). Fecal nitrogen output was reduced (P = 0.001) but urinary nitrogen output was unaffected (P = 0.691), while nitrogen retention (P = 0.077) and nitrogen retention/nitrogen intake (P = 0.077) tended to increase. Supplementary AL decreased methane emissions scaled to metabolic bodyweight by 5.95 % (P = 0.007) and to digestible organic matter intake by 8.36 % (P = 0.009). Ruminal pH was unaffected (P = 0.601) while ammonia decreased (P = 0.024) and total volatile fatty acids increased (P = 0.024) in response to supplementary AL. Supplementary AL decreased the population of methanogens (P = 0.001) and tended to decrease that of protozoans (P = 0.097), but increased the populations of F. succinogenes (P < 0.001), R. flavefaciens (P = 0.001), and B. fibrisolvens (P = 0.001).ConclusionsSupplementation of AL at 2.0 g/head·day effectively enhanced OM, N, NDF, and ADF digestibility and reduced daily methane emissions (L/kg BW0.75) in ewes, probably by decreasing the population of ruminal protozoans and methanogens.

Protein replacement by digestible fibre in the diet of growing rabbits: 2-Impact on performances, digestive health and nitrogen output

The fattening performances, nitrogen excretion and health status of growing rabbits were studied in response to a linear reduction of the digestible fibre (DgF=water-insoluble pectins+hemicelluloses) to crude protein ratio (DFP) in four diets (from 1.01 to 1.55). As the crude protein level was reduced from 17.7% to 13.9% (in diets DFP1–DFP4), the DgF level was increased from 18.5% to 22.1%, without changes to poorly-digestible fibre levels (ADF=19%). A total of 644 rabbits per diet were used in a multi-site study (n=6). Between weaning (28d–37d according to sites) and 49d of age, reduction of the DFP ratio led to a linear decrease in weight gain (−4g/d between DFP1 and DFP4, P<0.001), associated with a reduced feed intake (−5.7% between DFP1 and DFP4). However, for the whole fattening period, growth and intake did not differ significantly among the diets. Over the whole fattening period (35d–70d old), the mean faecal nitrogen output was of 1g/N per day and per rabbit, and remained similar for the four diets, while the urinary N output was reduced by 55% between DFP1 and DFP4 (P<0.001). Accordingly, the total N output per rabbit was of 82.4g, 75.5g, 63.2g and 57.4g (P<0.001) in animals fed with DFP1–DFP4 diets, respectively. The nitrogen output per kg of live weight of rabbit produced was 52.0g, 47.9g, 40.7g and 36.9g for DFP1–DFP4, respectively.At the same time, an increase of the DFP ratio of over 1.3 (CP<16% and DgF>20%) reduced the mortality by diarrhoea for the two highest DFP ratios when compared to the two lowest ratios (5.2% vs. 7.8%, P<0.05), and led to a 40% reduction of the health risk index between weaning and slaughtering (20.0% vs. 12.6% for DFP1 and DFP4, respectively, P<0.01). A significant reduction of mortality from rabbit epizootic enteropathy (3 sites) was also observed when the DFP ratio was increased to over 1.3. A reduction of the protein level in favour of a higher digestible-fibre level is therefore a useful feeding strategy which improves the resistance of the growing rabbit to enteropathy, without major impairment of performances, and with a reduced nitrogen output.

Performance and nutrients digestibility of piglets fed ditary detoxified balanites augyptiaca friuts

A feeding trial was conducted to assess the effect of dietary detoxified Nigerian Balanites aegyptiaca fruits on performance and nutrients digestibility of weanling pigs. Twenty pigs weighing averagely 4.5 ± 0.85kg were used for the experiment which lasted for one month. The pigs were randomly allocated to the five dietary treatments in a completely randomized design. Five diets made of a corn-soy control diet and four others to which 20% of boiled, roasted, boiled and roasted, boiled and fermented respectively were used. Feed consumption and weight gain of pigs maintained on the Balanites based diets were comparable with the conventional diet (p > 0.05). However, efficiency of feed utilization on the Balanites containing diets was inferior to that on the control diet (p < 0.05). Nutrients digestibility measured by dry organic matter and crude fiber were not significantly influenced (p > 0.05) but protein and fatdigestibility were significantly influenced by dietary Balanites (p < 0.05). Gross energy intake and daily retained energy were significant on Balanites containing diets (p < 0.05) relative to the reference diet while the metabolizable energy was not affected ( p > 0.05). Nitrogen consumption using indices of protein intake, fecal nitrogen output, daily retainednitrogen and nitrogen retention coefficient were not significantly different from values obtained on the control diet (p > 0.05). It was therefore concluded that Balanites aegyptiaca fruits, when detoxified by any of the traditional methods adopted, could be incorporated in diets for livestock even up to 70% inclusion level without deleterious effects on animal consuming the feed.

EFFECT OF DIETARY REPLACEMENT OF FISHMEAL PROTEIN BY SOYBEAN MEAL PROTEIN ON GROWTH PERFORMANCE, METABOLISM AND IMMUNITY OF GIBEL CARP (&lt;I&gt;CARASSIUS AURATUS GIBELIO&lt;/I&gt;)

A 16-weeks growth was conducted to evaluate the effect of replacement of dietary fishmeal by soybean meal on growth performance, feed utilization,nitrogen metabolism and immunity in gibel carp. Four isonitrogenous and isocaloric diets were formulated. Each diet was fed to triplicate groups of fish with the initial weight at about 2.32 g. Soybean meal was used to replace 0 (Control, D1), 20% (D2), 80% (D3) and 100% (D4) of dietary fishmeal protein. The fish was reared in a semi-recirculating system. During the experiment, water temperature was 23-30 degrees C, photoperiod was 12D:12L with the light period from 08 : 00 to 20: 00, dissolved oxygen was above 5 mg/L, aninionia-N (NH4+-N plus NH3-N) was less than 0.5 mg/L, pH was about 6.4. Fish were fed to satiation twice daily (9:00 and 15:00). At the beginning of the experiment, healthy fish ( initial body weight about 2.32 g) were batch weighed after 24h feed deprivation and randomly distributed into the 12 tanks (40 fish per tank). The tanks were randomly assigned the four diets. Fifty fish were taken from the remaining fish and frozen for initial fish body chemical analysis. During the experiment, an excess amount of feed was fed to fish and uneaten feed were collected after I It in each feeding, dried at 60 degrees C and reweighed. Leaching rate of uneaten feed in tanks was estimated by placing weighed feeds into a tank without fish for I It and then recovering, drying and reweighing. The average leaching rate was used to calibrate the amount of uneaten feed. Faeces were collected after uneaten feed collection at the start of the experiment and through all the experiment period after 7d. To minimize nutrient leaching in faeces, only fresh and intact faeces were collected. Faeces were dried at 70 degrees C for digestibility determination. At the end of the trial, the fish were starved for Id and batch weighed. Fish were killed by a blow on the head and blood samples were collected (24h after last feeding) from the caudal vein of six fish from each tank at the end of the feeding trial by using heparinized syringes. Blood was centrifuged at 3500 r/min for 15 min, plasma-separated and stored at -80 degrees C. The remaining fish in each tank were taken for final fish body composition analysis. The results showed that feeding rate (FR), specific growth rate (SGR), feed conversion efficiency (FCE), protein retention efficiency (PRE) and energy retention efficiency ( ERE) decreased significantly (p < 0.05). Apparent digestibility coefficient of protein (ADCp) significantly increased (p < 0.05), but ADC of dry matter and energy decreased (p < 0. 05). Total nitrogen intake (TNI), apparent nitrogen intake ( ANI), faecal nitrogen Output (FNO), non-faecal nitrogen excretion (NFE) and nitrogen retention (NE) significantly decreased with the increasing dietary soybean meal (p < 0.05, but nitrogen load (NL) significantly increased (p < 0.05). Levels of plasma glucose, triacylglycerol and superoxide dismutase activities increased significantly with the increasing dietary, soybean meal (p < 0.05), but plasma cholesterol concentration and lysozyme activities decreased (p < 0.05). In conclusion, the results from this study showed adverse effects of inclusion of the soybean meal in diets on growth performance, feed utilization, nitrogen metabolism and immunity in gibel carp. The palatability was not negatively affected when soybean meal was included in the diets.Unbalanced amino acid composition of soybean meal diets seem to be the main reason to influence growth performance and nitroge load of gibel carp.

The effect feeding forage legumes as nitrogen supplement on growth performance of sheep

The effect of feeding forage legumes, Cowpea, Silverleaf desmodium and Oxley fine stem stylo, as protein supplements to natural pasture (veld) hay on intake, growth rate and nitrogen metabolism in growing lambs was evaluated. Thirty growing lambs were stratified according to body weight and randomly assigned, within a stratum, to five diets in a completely randomised design. The diets were veld hay alone (V), veld hay supplemented with either 10 g/kg of urea (VU), veld hay supplemented with 250 g/kg Cowpea (VC), 250 g/kg Silverleaf desmodium (VS) or 250 g/kg Oxley fine stem stylo (VF) forage legume hays. The V and the VU groups were used as control diets. Animals supplemented with either urea or the forage legume had higher (P < 0.01) total dry matter intake compared with the animals on V. The animals supplemented with the forage legumes had higher (P < 0.01) nitrogen intake and faecal nitrogen output than the non-supplemented group. All animals, across the treatments, lost body weight; lambs on V had higher (P < 0.01) body weight losses than those in the other treatments. The forage legume supplemented groups lost less (P < 0.01) body weight than those on the V and VU diets. Although supplementation with forage legumes enhanced feed intake and reduced weight losses it did not maintain body weights of lambs fed a basal diet of poor quality roughages.

Combining unprotected pancreatic enzymes with pH-sensitive enteric-coated microspheres does not improve nutrient digestion in patients with cystic fibrosis

To assess the efficacy of combining unprotected powder enzymes and oral enteric-coated microsphere (ECM) and to ECM alone in treating nutrient maldigestion in patients with cystic fibrosis. Patients were randomly assigned into 2 consecutive, 2-week phases; ECM alone, and ECM plus unprotected powder enzymes. Fecal fat, energy, and nitrogen output were compared with intake at the end of each phase. Two-tailed, paired t tests were performed to compare outcomes. The mean age of the 14 patients (3 girls) was 5.7 +/- 3.2 years (range, 1.9 to 13.4 years). There was no significant difference in percent malabsorption of fat (15.6% vs 18.2%), energy (13.3% vs 13.4%), or nitrogen (11.8% vs 11.3%) between phases. The addition of powder enzymes to ECM did not improve nutrient maldigestion compared with ECM alone.

Subchronic oral toxicity studies with α-cyclodextrin in rats

The toxicity of α-cyclodextrin (α-CD), a cyclic polymer of six α-1,4-linked glucopyranosyl units with potential applications as a food ingredient, more specifically a water-soluble dietary fiber, was examined in a 4-week range finding study and a 13-week oral toxicity study in rats. In the 4-week study, the test substance was administered to groups of Bor:WISW(SPF;Cpb) rats at dietary levels of 0, 1, 5, and 15% (5 rats/sex/group). An additional group received a diet with 5% β-CD. In the 13-week study, groups of Crl:(WI)WU Br rats received diets with 0, 1.5, 5, or 20% α-CD. An additional group received a diet with 20% lactose (20 rats/sex/group). Satellite groups of 10 rats/sex were attached to the control, 20% α-CD and 20% lactose group. Following the 13-week treatment period, these satellite groups were kept on a standard, cereal-based rodent diet for a 4-week recovery period. Parameters measured during the two studies included clinical signs, body weights, food and water intake, hematological and clinicochemical parameters, and organ weights as well as gross and histopathological observations at necropsy. In the 13-week study, ophthalmoscopic examinations as well as urine and feces analyses were also conducted. There were no treatment-related mortalities in either study. In the 4-week study, persistent diarrhea was the most prominent, treatment-related effect observed in the animals of the 15% α-CD group especially in the male animals. In association with this effect, food consumption and food conversion efficiency were decreased. In line with observations from studies with other low-digestible, yet fermentable carbohydrates, the weight of the full and empty cecum was increased significantly in the 5% α-CD, 5% β-CD, and 15% α-CD group. The reduced relative liver weights (in males and females) and the significantly increased relative testes weight which were observed in the 15% α-CD group, were attributed to the impaired nutritional condition (due to diarrhea) and the reduced body weight of the animals of this group, respectively. Microscopic examination of the main organs did not reveal pathological alterations that could be attributed to the α-CD treatment. In the 13-week study, soft stool and infrequent mild diarrhea were observed only during the first 2–3 weeks in the 20% α-CD and 20% lactose group (mainly male animals). Accordingly, body weights were reduced in males of the 20% lactose group throughout the study and in the 20% α-CD group during the last week of the study. Food intakes were slightly increased in the 20% α-CD group and the food conversion efficiency, was significantly reduced in males, but not females, of the 20% α-CD and 20% lactose group. There were no treatment-related changes of hematological parameters. In line with similar observations from studies on other low-digestible carbohydrates, the urinary pH was decreased and urinary calcium levels increased in the 20% α-CD and 20% lactose group. Similarly, the fecal dry weight and nitrogen output was increased in these groups. At termination of the treatment, significantly in creased cecum weights (full and empty) were observed in the 5 and 20% α-CD groups and the 20% lactose group. The relative (not absolute) weight of the spleen was significantly increased in males of the 20% α-CD group. In the 20% lactose group, the relative weights of the spleen and liver (females) and the testes, brain, and adrenals (males) were significantly increased. The histopathological examination of these and all other organs and tissues did not reveal any abnormalities that could be attributed to the α-CD or lactose treatment. In conclusion, the ingestion of α-CD for 13-weeks at dietary levels of up to 20% (corresponding to intakes of 12.6 and 13.9 g/kg bodyweight/d in male and female rats, respectively) did not produce any signs of toxicity or adverse effects.

Proteínas do feijão preto sem casca: digestibilidade em animais convencionais e isentos de germes (germ-free)

O feijão (Phaseolus vulgaris, L. ) é uma leguminosa de grande importância para a dieta da população brasileira. No entanto, um de seus maiores problemas é representado pelo baixo valor nutricional de suas proteínas, decorrente, por um lado, da sua baixa digestibilidade e, de outro, do teor e biodisponibilidade reduzidos de aminoácidos sulfurados. Com o objetivo de avaliar a digestibilidade das proteínas albumina e globulina do feijão preto sem casca, foram realizados ensaios biológicos com camundongos isentos de germes e convencionais e com ratos (Wistar), recém-desmamados, com idade de 21 a 25 dias. Avaliou-se ainda o Escore Químico Corrigido pela Digestibilidade da Proteína. A digestibilidade verdadeira no experimento com camundongos isentos de germes foi de 90,21 e 90,00%, no teste com camundongos convencionais foi de 85,53 e 86,73%, e no experimento com ratos foi de 82,62 e 68,53%, para albumina e globulina, respectivamente. O Escore Químico Corrigido pela Digestibilidade da Proteína foi de 61,00% para a albumina e 51,00% para a globulina. A digestibilidade determinada em animais isentos de germes foi superior aos valores encontrados em animais convencionais, sugerindo que a flora intestinal esteja contribuindo para elevar o teor de nitrogênio nas fezes dos animais convencionais, e, portanto, esteja sendo subestimada a digestibilidade verdadeira do feijão.