Interest point detection methods are gaining more attention and are widely applied in computer vision tasks such as image retrieval and 3D reconstruction. However, there still exist two main problems to be solved: (1) from the perspective of mathematical representations, the differences among edges, corners, and blobs have not been convincingly explained and the relationships among the amplitude response, scale factor, and filtering orientation for interest points have not been thoroughly explained; (2) the existing design mechanism for interest point detection does not show how to accurately obtain intensity variation information on corners and blobs. In this paper, the first- and second-order Gaussian directional derivative representations of a step edge, four common genres of corners, an anisotropic-type blob, and an isotropic-type blob are analyzed and derived. Multiple interest point characteristics are discovered. The characteristics for interest points that we obtained help us describe the differences among edges, corners, and blobs, explain why the existing interest point detection methods with multiple scales cannot properly obtain interest points from images, and present novel corner and blob detection methods. Extensive experiments demonstrate the superiority of our proposed methods in terms of detection performance, robustness to affine transformations, noise, image matching, and 3D reconstruction.