FDR Values Research Articles

Dana Pihak Ketiga dan Modal Sendiri terhadap Financing to Deposit Ratio di PT Bank BRI Syariah

This research was conducted because the development of TPF and Equity Capital at PT Bank BRI Syariah for the 2016-2020 period fluctuated. TPF in 2016 in the third quarter and fourth quarter experienced an increase but FDR decreased as well as fluctuations in TPF during 2018 and 2020 which did not match the theory, where the higher the DPK collected, the greater the distribution of financing so that the FDR value would increase and vice versa. This study aims to determine the effect of TPF on FDR, the effect of Equity Capital on FDR, the effect of TPF and Equity Capital simultaneously on FDR at PT Bank BRI Syariah. This study uses associative quantitative research, with the object of this research being Third Party Funds, Own Capital and Financing to Deposit Ratio at PT Bank BRI Syariah. The type of data used is secondary data in the form of financial ratios published by PT. Bank BRI Syariah starting from the period 2016 to 2020. The population in this study is the financial statements of PT Bank BRI Syariah. The sample used is the quarterly published financial statements of BRI Syariah Bank for the period 2016 to 2020. The data analysis used is multiple correlation analysis, multiple regression analysis, coefficient of determination analysis, t-test and F-test. Own capital has a positive and significant effect on FDR. TPF and own capital simultaneously have a positive and significant effect on FDR at PT Bank BRI Syariah.

Dana Pihak Ketiga dan Modal Sendiri terhadap Financing to Deposit Ratio di PT Bank BRI Syariah

This research was conducted because the development of TPF and Equity Capital at PT Bank BRI Syariah for the 2016-2020 period fluctuated. TPF in 2016 in the third quarter and fourth quarter experienced an increase but FDR decreased as well as fluctuations in TPF during 2018 and 2020 which did not match the theory, where the higher the DPK collected, the greater the distribution of financing so that the FDR value would increase and vice versa. This study aims to determine the effect of TPF on FDR, the effect of Equity Capital on FDR, the effect of TPF and Equity Capital simultaneously on FDR at PT Bank BRI Syariah. This study uses associative quantitative research, with the object of this research being Third Party Funds, Own Capital and Financing to Deposit Ratio at PT Bank BRI Syariah. The type of data used is secondary data in the form of financial ratios published by PT. Bank BRI Syariah starting from the period 2016 to 2020. The population in this study is the financial statements of PT Bank BRI Syariah. The sample used is the quarterly published financial statements of BRI Syariah Bank for the period 2016 to 2020. The data analysis used is multiple correlation analysis, multiple regression analysis, coefficient of determination analysis, t-test and F-test. Own capital has a positive and significant effect on FDR. TPF and own capital simultaneously have a positive and significant effect on FDR at PT Bank BRI Syariah.

BCL7B is a potential novel diagnosis and prognosis biomarker for sarcomas using bioinformatics analysis.

BCL7B plays a potential role in the progression of various cancers, while its role in sarcomas is unknown. We aimed to evaluate BCL7B's diagnostic and prognostic value, and potential BCL7B-related mechanisms in sarcomas based on The Cancer Genome Atlas (TCGA) database. We collected patients with sarcoma from TCGA. Wilcoxon rank sum test was used to compare the expression of BCL7B in sarcoma samples with different clinical-pathologic features. Univariate Cox regression analysis and multivariate Cox regression analysis were used to evaluate prognosis factors for sarcoma. Gene set enrichment analysis (GSEA) was conducted to elucidate the significant functions and pathways associated with BCL7B. BCL7B was a potential biomarker for distinguishing normal and tumor tissues with the analysis of ROC curve (AUC = 0.588). Low BCL7B expression was significantly correlated with tumor multifocal (OR = 0.39 for yes vs no), larger residual tumor (OR = 0.40 for R1,R2 vs RO), male gender (OR = 0.48 for male vs female) and White race (OR = 0.29 for White vs Asian, Black or African American). High BCL7B expression was correlated with leiomyosarcoma histological type (OR = 6.08 for leiomyosarcoma vs dedifferentiated liposarcoma, pleomorphic sarcoma). Univariate and multivariate Cox regression analysis showed that low BCL7B expression was independently associated with poor overall survival (P = .008). GSEA showed that GPCR (G protein-coupled receptors) ligand binding, secreted factors, class A1 rhodopsin-like receptors, extracellular matrix organization, core matrisome, Fc epsilon receptor I mediated NF-κB activation, and WNT signaling pathway were differentially enriched in BCL7B low expression phenotype (|NES| > 1, adjusted P value <.05, and FDR value <0.25). BCL7B may play an important role in sarcoma progression and may be a potential biomarker for prognosis and diagnosis in sarcomas.

EMPIRICAL STUDIES OF THE EFFECT OF OPERATIONAL COSTS AND OPERATING INCOME, FINANCING TO DEPOSIT RATIO AGAINST RETURN ON ASSET WITH NON-PERFORMING FINANCING AS INTERVENING VARIABLES IN SHARIA BANK INDONESIA 2013-2020

Return On Asset is a profitability ratio that can measure the company's ability to produce labs. In this study, the researchers found data on the quarterly financial reports of Bank Syariah Indonesia that the FDR value increases. However, the ROA value and decreased, found the BOPO and NPF values decreased but were not accompanied by an increase in ROA. This study using a quantitative method with an associative approach. The type of data used is secondary data from the 2013 first-quarter financial statements - In the fourth quarter of 2020, data is taken from the Bank Syariah Indonesia website. Data analysis using classical assumption test, simple linear regression analysis, linear regression analysis multiple, hypothesis testing, and path analysis. The results showed that NPF could mediate between BOPO and ROA. NPF can mediate between FDR and ROA. The cause of the decrease in ROA is caused because the BOPO value is still relatively high due to Sharia Bank Indonesia's performance less efficient and have the FDR value in several quarters exceed the maximum limit. So it indicates that the bank is less efficient in the distribution of financing and has an NPF value that exceeds the limit set by Bank Indonesia in several quarters. The solution is to make BOPO efficient. Indonesian Sharia Banks must pay attention to operational efficiency by paying attention to costs incurred with revenue received. A low BOPO will significantly affect the level of profit Islamic banks to improve the quality of ROA. Furthermore, more cost-effectiveness further increases the income so that the profit obtained is higher. Next, Bank Syariah Indonesia must use the number of funds in the banking sector to channel financing so that the risk of financing is getting higher reduced.

Perbedaan NPF dan FDR Bank Muamalat antara Sebelum dan Selama Pandemi Covid-19

This research was conducted to analyze and describe the NPF and FDR ratio of Bank Muamalat Indonesia between before and during the Covid-19 pandemic. The method used in this research is quantitative with research data in the form of secondary data. The data were collected using the documentation method and analyzed using the descriptive statistic and independent sample t-test. The results of the study state that the NPF and FDR value of Bank Muamalat Indonesia was differ between before and during the Covid-19 pandemic, although not statistically significant. The difference in terms of NPF indicates that the financing risk faced by Bank Muamalat Indonesia during the pandemic period is greater. The difference in terms of FDR indicates the existence of prudence from banks in distributing financing to the public because the business situation is not conducive during the Covid-19 pandemic.

Perbedaan NPF dan FDR Bank Muamalat antara Sebelum dan Selama Pandemi Covid-19

This research was conducted to analyze and describe the NPF and FDR ratio of Bank Muamalat Indonesia between before and during the Covid-19 pandemic. The method used in this research is quantitative with research data in the form of secondary data. The data were collected using the documentation method and analyzed using the descriptive statistic and independent sample t-test. The results of the study state that the NPF and FDR value of Bank Muamalat Indonesia was differ between before and during the Covid-19 pandemic, although not statistically significant. The difference in terms of NPF indicates that the financing risk faced by Bank Muamalat Indonesia during the pandemic period is greater. The difference in terms of FDR indicates the existence of prudence from banks in distributing financing to the public because the business situation is not conducive during the Covid-19 pandemic.

Capital Adequacy Ratio (Car) dan Faktor-Faktor Yang Mempengaruhinya di Bank Syariah Mandiri (Periode Maret 2012 - Maret 2019)

CAR variable is the most important indicator of bank health according to BI. CAR is influenced by NPF, ROA, BOPO, FDR. The problem is how partially the influence of NPF, ROA, BOPO, FDR on CAR in BSM from March 2012-March 2019? Do NPF, ROA, BOPO, FDR simultaneously affect CAR? The research method used is quantitative descriptive with SPPS analysis version 22. The results showed that the NPF value of t-count was 0.449 &lt;t table 1.705, so that it received Ho1 so that it could be concluded that NPF had no effect on CAR at PT. BSM for March 2012-March 2019. While ROA, BOPO and FDR affect the CAR of PT. BSM. This can be seen from the value of each variable at the t-value higher than the t-table value. ROA t-count 2,262&gt; 1,705 t-tables. BOPO value of t-itung 1.916&gt; t-table 1.705. and FDR value t-count -2.969&gt; t-table 1.705, and for the simultaneous test F count&gt; F table (3.286&gt; 2.98) with a significant value of 0.028&gt; α = 5% (0.05). This means that there is a significant influence together between all the independent variables on the dependent variable in PT.BSM.

Impact of autism-associated genetic variants in interaction with environmental factors on ADHD comorbidities: an exploratory pilot study.

Attention-deficit/hyperactivity disorder (ADHD) is determined by genetic and environmental factors, and shares genetic risk with ASD. Functional single-nucleotide polymorphisms of the metabotropic glutamatergic signaling pathway are reported to increase the risk for ASD. The aim of this pilot study was to explore the main effects of respective ASD variants as well as their interaction effects with well-replicated ADHD environmental risk factors on the risk for ADHD, ADHD symptom severities, and comorbidities. We included 318 children with ADHD, aged 5-13years, and their parents (N = 164 trios, N = 113 duos, N = 41 singletons). Interaction of ASD risk variants CYFIP1-rs7170637, CYFIP1-rs3693, CAMK4-rs25925, and GRM1-rs6923492 with prenatal biological and lifetime psychosocial risk factors was explored in a subsample with complete environmental risk factors (N = 139 trios, N = 83 duos, two singletons) by transmission disequilibrium test and stepwise regression analyses. We identified nominally significant (alpha < 0.05) GxE interactions of acute life events with CYFIP1-rs3693 on ADHD diagnosis (p = 0.004; fdr = 0.096) but no significant association of any single marker. Further results suggest that the risk for comorbid disruptive disorders was significantly modulated by GxE interactions between familial risk factors and CAMK4-rs25925 (p = 0.001; fdr = 0.018) and prenatal alcohol exposure with CYFIP1-rs3693 (p = 0.003; fdr = 0.027); both findings survived correction for multiple testing (fdr value < 0.05). Nominal significant GxE interactions moderating the risk for anxiety disorders have also been identified, but did not pass multiple testing corrections. This pilot study suggests that common ASD variants of the glutamatergic system interact with prenatal and lifetime psychosocial risk factors influencing the risk for ADHD common comorbidities and thus warrants replication in larger samples.

False Discovery Rate Estimation for Hybrid Mass Spectral Library Search Identifications in Bottom-up Proteomics.

We present a method for FDR estimation of mass spectral library search identifications made by a recently developed method for peptide identification, the hybrid search, based on an extension of the target-decoy approach. In addition to estimating confidence for a given identification, this allows users to compare and integrate identifications from the hybrid mass spectral library search method with other peptide identification methods, such as a sequence database-based method. In addition to a score, each hybrid score is associated with a "DeltaMass" value, which is the difference in mass of the search and library peptide, which can correspond to the mass of a modification. We explored the relation between FDR and DeltaMass using 100 concatenated random decoy libraries and discovered that a small number of DeltaMass values were especially likely to result from decoy searches. Using these values, FDR values could be adjusted for these specific values and a reliable FDR generated for any DeltaMass value. Finally, using this method, we find and examine common, reliable identifications made by the hybrid search for a range of proteomic studies.

Abstract 2669: A role for HOXA5 in the transcriptional response of ovarian and fallopian tube surface epithelial cells to norepinephrine

Abstract Chronic stress has been suggested to play a role in tumor initiation, progression and patient survival. Studies have shown that activation of the sympathetic nervous system and increased levels of stress-associated hormones such as norepinephrine can enhance migration, invasion and growth of ovarian cancer cells. However, the extent to which stress contributes to tumor initiation remains unknown. The purpose of this study was to explore the effects of norepinephrine on the transcriptional program of immortalized normal cell lines presumed to be the origin of epithelial ovarian cancers. We treated normal immortalized ovarian (iOSE) and fallopian tube (iFTE) surface epithelial cells with 10uM norepinephrine for 15 min, 1h and 4h and generated global transcriptomic profile of treated vs untreated cells by RNA-Seq. The most significant gene expression changes (P(adj) &amp;lt; 0.05) were observed at the 1h time point; with an overlap of 45 differentially expressed genes in both iOSE and iFTE cells. Genes in immune-related and developmental pathways were found to be highly enriched in the norepinephrine treated samples set. By the 4h time point, the number of differentially expressed genes had increased to 313 genes from 234 at 1h in iFTE cells, while it reduced to 34 genes from 53 in iOSE cells. Additionally, the number of differentially expressed genes common to iOSE and iFTE cells had reduced to 14. Gene ontology analysis of the differentially expressed genes revealed ‘transcription factor’ to be the most significantly overrepresented class (based on FDR value) at both 1h and 4h time-points. Promoter enrichment analysis identified HOXA5, GFI1 and FOXI1 transcription factor binding sites to be highly enriched (based on Z-score and Fisher-Score) at both time points. HOXA5 ranked consistently at the top in both cell lines and at both 1h and 4h time points. These observations suggest that HOXA5 may play a central role in regulating the early response to norepinephrine in normal ovarian and fallopian tube cells. We will further assess the function of HOXA5 through ChIP-Seq and shRNA- mediated silencing. Citation Format: Sweta Dash, Anxhela Gjyshi, Ling Cen, Chia-Ho Cheng, Chaomei Zhang, Sean J. Yoder, Jamie K. Teer, Guillermo N. Armaiz-Pena, Alvaro N. Monteiro. A role for HOXA5 in the transcriptional response of ovarian and fallopian tube surface epithelial cells to norepinephrine [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 2669.

Muscle fiber hypertrophy in response to 6 weeks of high-volume resistance training in trained young men is largely attributed to sarcoplasmic hypertrophy.

Cellular adaptations that occur during skeletal muscle hypertrophy in response to high-volume resistance training are not well-characterized. Therefore, we sought to explore how actin, myosin, sarcoplasmic protein, mitochondrial, and glycogen concentrations were altered in individuals that exhibited mean skeletal muscle fiber cross-sectional area (fCSA) hypertrophy following 6 weeks of high-volume resistance training. Thirty previously resistance-trained, college-aged males (mean ± standard deviation: 21±2 years, 5±3 training years) had vastus lateralis (VL) muscle biopsies obtained prior to training (PRE), at week 3 (W3), and at week 6 (W6). Muscle tissue from 15 subjects exhibiting PRE to W6 VL mean fCSA increases ranging from 320–1600 μm2 was further interrogated using various biochemical and histological assays as well as proteomic analysis. Seven of these individuals donated a VL biopsy after refraining from training 8 days following the last training session (W7) to determine how deloading affected biomarkers. The 15 fCSA hypertrophic responders experienced a +23% increase in mean fCSA from PRE to W6 (p<0.001) and, while muscle glycogen concentrations remained unaltered, citrate synthase activity levels decreased by 24% (p<0.001) suggesting mitochondrial volume decreased. Interestingly, repeated measures ANOVAs indicated that p-values approached statistical significance for both myosin and actin (p = 0.052 and p = 0.055, respectively), and forced post hoc tests indicated concentrations for both proteins decreased ~30% from PRE to W6 (p<0.05 for each target). Phalloidin-actin staining similarly revealed actin concentrations per fiber decreased from PRE to W6. Proteomic analysis of the sarcoplasmic fraction from PRE to W6 indicated 40 proteins were up-regulated (p<0.05), KEGG analysis indicated that the glycolysis/gluconeogenesis pathway was upregulated (FDR sig. <0.001), and DAVID indicated that the following functionally-annotated pathways were upregulated (FDR value <0.05): a) glycolysis (8 proteins), b) acetylation (23 proteins), c) gluconeogenesis (5 proteins) and d) cytoplasm (20 proteins). At W7, sarcoplasmic protein concentrations remained higher than PRE (+66%, p<0.05), and both actin and myosin concentrations remained lower than PRE (~-50%, p<0.05). These data suggest that short-term high-volume resistance training may: a) reduce muscle fiber actin and myosin protein concentrations in spite of increasing fCSA, and b) promote sarcoplasmic expansion coincident with a coordinated up-regulation of sarcoplasmic proteins involved in glycolysis and other metabolic processes related to ATP generation. Interestingly, these effects seem to persist up to 8 days following training.

Associations between olfactory pathway gene methylation marks, obesity features and dietary intakes

BackgroundOlfaction is an important sense influencing food preferences, appetite, and eating behaviors. This hypothesis-driven study aimed to assess associations between olfactory pathway gene methylation signatures, obesity features, and dietary intakes.MethodsA nutriepigenomic analysis was conducted in 474 adults from the Methyl Epigenome Network Association (MENA) project. Anthropometric measurements, clinical data, and serum metabolic profiles of the study population were obtained from structured databases of the MENA cohorts. Habitual dietary intake was assessed using a validated semiquantitative food frequency questionnaire. DNA methylation was measured in circulating white blood cells by microarray (Infinium Human Methylation 450 K BeadChips). FDR values (p < 0.0001) were used to select those CpGs that showed the best correlation with body mass index (BMI) and waist circumference (WC). Pathway analyses involving the characterization of genes involved in the olfactory transduction system were performed using KEGG and pathDIP reference databases.ResultsOverall, 15 CpG sites at olfactory pathway genes were associated with BMI (p < 0.0001) and WC (p < 0.0001) after adjustments for potential confounding factors. Together, methylation levels at the15 CpG sites accounted for 22% and 20% of the variability in BMI and WC (r2 = 0.219, p < 0.001, and r2 = 0.204, p < 0.001, respectively). These genes encompassed olfactory receptors (OR4D2, OR51A7, OR2T34, and OR2Y1) and several downstream signaling molecules (SLC8A1, ANO2, PDE2A, CALML3, GNG7, CALML6, PRKG1, and CAMK2D), which significantly regulated odor detection and signal transduction processes within the complete olfactory cascade, as revealed by pathway enrichment analyses (p = 1.94 × 10–10). Moreover, OR4D2 and OR2Y1 gene methylation patterns strongly correlated with daily intakes of total energy (p < 0.0001), carbohydrates (p < 0.0001), protein (p < 0.0001), and fat (p < 0.0001).ConclusionsThe results of this study suggest novel relationships between olfactory pathway gene methylation signatures, obesity indices, and dietary intakes.

A Novel Classification Method using Effective Neural Network and Quantitative Magnetization Transfer Imaging of Brain White Matter in Relapsing Remitting Multiple Sclerosis

Quantitative Magnetization Transfer Imaging (QMTI) is often used to quantify the myelin content in multiple sclerosis (MS) lesions and normal appearing brain tissues. Also, automated classifiers such as artificial neural networks (ANNs) can significantly improve the identification and classification processes of MS clinical datasets. We classified patients with relapsing-remitting multiple sclerosis (RRMS) from healthy subjects using QMTI and T1 longitudinal relaxation time data of brain white matter, then the performance of three ANN-based classifiers have been investigated. The input features of ANN algorithms, including multilayer perceptron (MLP), radial basis function (RBF) and ensemble neural networks based on Akaike information criterion (ENN-AIC) were extracted in the form of QMTI and T1 mean values from parametric maps. The ANNs quantitative performance is measured by the standard evaluation of confusion matrix criteria. The results indicate that ENN-AIC-based classification method has achieved 90% accuracy, 92% sensitivity and 86% precision compared to other ANN models. NPV, FPR and FDR values were found to be 0.933, 0.125 and 0.133, respectively, according to the proposed ENN-AIC model. A graphical representation of how to track actual data by the predictive values derived from ANN algorithms, was also presented. It has been demonstrated that ENN-AIC as an effective neural network improves the quality of classification results compared to MLP and RBF.In addition, this research provides a new direction to classify a large amount of quantitative MRI data that can help the physician in a correct MS diagnosis.

Effect of cold storage and different ions on the thermal resistance of B. cereus NZAS01 spores- analysis of differential gene expression and ion exchange

Bacillus cereus spores in food are able to survive pasteurization, and if conditions are favourable, subsequently germinate, grow and produce toxins causing food poisoning. The objectives of this study were to firstly determine the impact of cold storage and ion uptake on the thermal resistance of B. cereus spores and secondly to use differential gene expression to help elucidate possible molecular mechanisms for the changes detected in their thermal resistance. B. cereus spores were held at 4 °C in either 0.05 or 0.5 M solutions of cations (Na+, Ca2+ Mg2+,K+, Zn2+) for 6 days and their D88-values were estimated. In the presence of sodium chloride (0.05 and 0.5 M), sodium phosphate buffer, (pH 7, 0.05 and 0.5 M) or zinc acetate (0.05 M), D88 values decreased by 8.8, 10.9, 11.2, 12.9, and 10.2 min respectively, with no evidence of germination (plating methods). Exposure of spores to Na+ in sodium phosphate buffer (pH 7, 0.05 and 0.5 M) or sodium chloride (0.05 and 0.5 M) resulted in the accumulation of Na+ (66.0 ± 2.9, 193.1 ± 4.6, 136.2 ± 9.9 and 70.5 ± 2.7 μg/g) by spores at the significant expense of K+ (10.8 ± 0.5, 7.5 ± 0.2, 8.1 ± 0.4 and 3.6 ± 0.4 μg/g respectively). The mechanism behind the loss of resistance in sodium phosphate buffer (0.05 M) was further investigated by monitoring the differential gene expression using mRNA sequencing. Genes encoding for uracil permease (BC_3890), Mg2+ P-type ATPase-like protein (BC_1581), ABC transporter ATP-binding protein (BC_0815), and 2-keto-3-deoxygluconate permease (BC_4841) were significantly (FDR value ≤0.05) upregulated. This upregulation indicated a possible increase in permeability, which is suggested to account for the increased uptake of sodium ions and the reduction measured in the spore's thermal resistance. This data suggests that during storage at 4 °C in the presence of sodium ions, spores should not be considered to be completely dormant.

Abstract 1436: Beta-caryophyllene regulates lipid biosynthesis in breast cancer cells

Abstract The search for therapeutic anti-cancer drugs has spanned both synthetic and natural products approaches, and notable success has been achieved from unique chemistries produced by plants. Early studies in E.coli showed that cyclic hydrocarbons, including terpenes, interact directly with biological membranes. Accumulation of hydrocarbons results in membrane swelling and increased membrane fluidity, both signs of cell stress. At biological temperatures, membrane fluidity is controlled by the saturation state of the acyl chains of fatty acids (primarily in phospholipids) and cholesterol content. Changes in either of these parameters leads to membrane remodeling which can affect membrane function. Terpenes are, themselves, precursors to complex sterols across all kingdoms of life. Beta-caryophylene (BCP), a bicyclic sesquiterpene, induces cell death across a variety of cancer cell types, although the mechanism(s) by which this occurs is not completely known. Our data show that BCP induces membrane permeability in breast cancer lines representing both ER-positive and triple negative phenotypes (TNBC), as measured by lactate dehydrogenase release from cells. We sought to understand this phenomenon by looking at changes in the transcriptome of treated cells compared to controls. We chose to examine cells exposed to hypoxia, rationalizing that this condition, in vivo, creates an aggressive phenotype, is associated with TNBC and drug-resistant (recurrent) breast cancers, and is an independent prognosticator for poor patient outcome. We isolated high quality RNA (RIN&amp;gt;9) at the University of Florida. The Genomics Core at the University of Louisville prepared libraries and performed the sequencing run (Illumina NextSeq 500). This generated over 144 million 75bp reads that aligned to the human genome (96.3% alignment rate), or approximately 24 million reads per sample. As a first approach in data analysis, we selected differential expression based on the FDR adjusted p-values (q-values) &amp;lt;0.05 (as determined by CuffDiff), and analyzed this gene set for pathway involvement using the reactome data base. Reactome also assesses FDR values for pathway analysis, and we selected pathway FDR &amp;lt;0.05 providing the highest level of specificity. This search modality revealed an orchestrated up-regulation of lipid metabolism, including that of cholesterol biosynthesis. This is consistent with BCP-induced membrane remodeling. We have validated these results, not by PCR, but through protein expression and metabolic assays. Because these events occur at concentrations that are sub-cytotoxic, this potentially reveals a new mechanism underlying the development of drug resistance. Citation Format: Mam Y. Mboge, Adam P. Bullock, Riley O'Dennell, John V. Matthias, Julie A. Davila, Christopher J. Frost, Susan C. Frost. Beta-caryophyllene regulates lipid biosynthesis in breast cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 1436.

Genome-wide screening differential long non-coding RNAs expression profiles discloses its roles involved in OHSS development.

To screen differentially expressed lncRNAs involved in OHSS. OHSS is defined as ovarian hyperstimulation syndrome. It is characterized as enlarged ovary and increased vascular permeability. Case-control study. University-affiliated hospital. Patients with OHSS high risk (n = 30) and low risk (n = 30) were included in this study. None. LncRNAs from women with OHSS high risk and low risk were used for high-throughput sequencing profiling. The eight most differentially expressed lncRNAs in granulosa cells were validated by semi-quantitative reverse transcription-polymerase chain reaction analysis. A total of 23,815 lncRNAs were detected and 482 were differentially expressed (fold-change ≥2; p < 0.05, FDR value < 0.001), of which 205 were upregulated and 277 were downregulated. Lnc-SEC16B.1-6, lnc-SNURF-13, lnc-LGR6-6, and lnc-H2AFY2-2 were up-regulated, while lnc-BRD2-2, lnc-HSPA6-2, and lnc-CLIC6-5 were downregulated significantly in granulosa cells. These results were confirmed by qRT-PCR. KEGG pathways and Gene Ontology enrichment analysis revealed that several biological processes were significantly associated. Meanwhile, the lncRNA/miRNA interaction network was established according to ceRNA network model. Comprehensive expression screening identified eight novel lncRNAs associated with risk factors of OHSS process. Although it is unclear how these altered lncRNAs regulate the process of OHSS, our findings suggest these lncRNAs may be novel players in OHSS development.

FUS Mutant Human Motoneurons Display Altered Transcriptome and microRNA Pathways with Implications for ALS Pathogenesis.

SummaryThe FUS gene has been linked to amyotrophic lateral sclerosis (ALS). FUS is a ubiquitous RNA-binding protein, and the mechanisms leading to selective motoneuron loss downstream of ALS-linked mutations are largely unknown. We report the transcriptome analysis of human purified motoneurons, obtained from FUS wild-type or mutant isogenic induced pluripotent stem cells (iPSCs). Gene ontology analysis of differentially expressed genes identified significant enrichment of pathways previously associated to sporadic ALS and other neurological diseases. Several microRNAs (miRNAs) were also deregulated in FUS mutant motoneurons, including miR-375, involved in motoneuron survival. We report that relevant targets of miR-375, including the neural RNA-binding protein ELAVL4 and apoptotic factors, are aberrantly increased in FUS mutant motoneurons. Characterization of transcriptome changes in the cell type primarily affected by the disease contributes to the definition of the pathogenic mechanisms of FUS-linked ALS.

DNA methylation patterns at sweet taste transducing genes are associated with BMI and carbohydrate intake in an adult population

Individual differences in taste perception may influence appetite, dietary intakes, and subsequently, disease risk. Correlations of DNA methylation patterns at taste transducing genes with BMI and dietary intakes were studied. A nutriepigenomic analysis within the Methyl Epigenome Network Association (MENA) project was conducted in 474 adults. DNA methylation in peripheral white blood cells was analyzed by a microarray approach. KEGG pathway analyses were performed concerning the characterization and discrimination of genes involved in the taste transduction pathway. Adjusted FDR values (p<0.0001) were used to select those CpGs that showed best correlation with BMI. A total of 29 CpGs at taste transducing genes met the FDR criteria. However, only 12 CpGs remained statistically significant after linear regression analyses adjusted for age and sex. These included cg15743657 (TAS1R2), cg02743674 (TRPM5), cg01790523 (SCN9A), cg15947487 (CALHM1), cg11658986 (ADCY6), cg04149773 (ADCY6), cg02841941 (P2RY1), cg02315111 (P2RX2), cg08273233 (HTR1E), cg14523238 (GABBR2), cg12315353 (GABBR1) and cg05579652 (CACNA1C). Interestingly, most of them were implicated in the sweet taste signaling pathway, except CACNA1C (sour taste). In addition, TAS1R2 methylation at cg15743657 was strongly correlated with total energy (p<0.0001) and carbohydrate intakes (p<0.0001). This study suggests that methylation in genes related to sweet taste could be an epigenetic mechanism associated with obesity.

Simulated herbivory in chickpea causes rapid changes in defense pathways and hormonal transcription networks of JA/ethylene/GA/auxin within minutes of wounding

Chickpea (C. arietinum L.) is an important pulse crop in Asian and African countries that suffers significant yield losses due to attacks by insects like H. armigera. To obtain insights into early responses of chickpea to insect attack, a transcriptomic analysis of chickpea leaves just 20 minutes after simulated herbivory was performed, using oral secretions of H. armigera coupled with mechanical wounding. Expression profiles revealed differential regulation of 8.4% of the total leaf transcriptome with 1334 genes up-regulated and 501 down-regulated upon wounding at log2-fold change (|FC| ≤ −1 and ≥1) and FDR value ≤ 0.05. In silico analysis showed the activation of defenses through up-regulation of genes of the phenylpropanoid pathway, pathogenesis, oxidases and CYTP450 besides differential regulation of kinases, phosphatases and transcription factors of the WRKY, MYB, ERFs, bZIP families. A substantial change in the regulation of hormonal networks was observed with up-regulation of JA and ethylene pathways and suppression of growth associated hormone pathways like GA and auxin within 20 minutes of wounding. Secondary qPCR comparison of selected genes showed that oral secretions often increased differential expression relative to mechanical damage alone. The studies provide new insights into early wound responses in chickpea.

An investigation of obesity susceptibility genes in Northern Han Chinese by targeted resequencing

Our earlier genome-wide linkage study of body mass index (BMI) showed strong signals from 7q36.3 and 8q21.13. This case-control study set to investigate 2 genomic regions which may harbor variants contributed to development of obesity.We employed targeted resequencing technology to detect single nucleotide polymorphisms (SNPs) in 7q36.3 and 8q21.13 from 16 individuals with obesity. These were compared with 504 East Asians in the 1000 Genomes Project as a reference panel. Linkage disequilibrium (LD) block analysis was performed for the significant SNPs located near the same gene. Genes involved in statistically significant loci were then subject to gene set enrichment analysis (GSEA).The 16 individuals aged between 30 and 60 years with BMI = 33.25 ± 2.22 kg/m. A total of 12,131 genetic variants across all of samples were found. After correcting for multiple testing, 65 SNPs from 25 nearest genes (INSIG1, FABP5, PTPRN2, VIPR2, WDR60, SHH, UBE3C, LMBR1, PAG1, IMPA1, CHMP4, SNX16, BLACE, EN2, CNPY1, LOC100506302, RBM33, LOC389602, LOC285889, LINC01006, NOM1, DNAJB6, LOC101927914, ESYT2, LINC00689) were associated with obesity at significant level q-value ≤ 0.05. LD block analysis showed there were 10 pairs of loci with D' ≥ 0.8 and r ≥ 0.8. GSEA further identified 2 major related gene sets, involving lipid raft and lipid metabolic process, with FDR values <0.12 and <0.4, respectively.Our data are the first documentation of genetic variants in 7q36.3 and 8q21.13 associated with obesity using target capture sequencing and Northern Han Chinese samples. Additional replication and functional studies are merited to validate our findings.