Favorable Curative Effect Research Articles

Urchin-like ceria nanoparticles for enhanced gene therapy of osteoarthritis.

Osteoarthritis (OA) is the most common degenerative joint disease in the world. Gene therapy based on delivering microRNAs (miRNAs) into cells has potential for the treatment of OA. However, the effects of miRNAs are limited by the poor cellular uptake and stability. Here, we first identify a type of microRNA-224-5p (miR-224-5p) from clinical samples of patients with OA that can protect articular cartilage from degeneration and further synthesize urchin-like ceria nanoparticles (NPs) that can load miR-224-5p for enhanced gene therapy of OA. Compared with traditional sphere ceria NPs, the thorns of urchin-like ceria NPs can efficiently promote the transfection of miR-224-5p. In addition, urchin-like ceria NPs have excellent performance of scavenging reactive oxygen species (ROS), which can regulate the microenvironment of OA to further improve the gene treatment of OA. The combination of urchin-like ceria NPs and miR-224-5p not only exhibits favorable curative effect for OA but also provides a promising paradigm for translational medicine.

Function of tumor infiltrating lymphocytes in solid tumors - a review

Tumor is one of the major diseases threatening human health in the 21st century. Surgical resection, radiotherapy, chemotherapy and targeted therapy are the main clinical treatments for solid tumors. However, these methods are unable to eradicate tumor cells completely, and easily lead to the recurrence and progression of tumor. Tumor immunotherapy is a novel treatment that uses human immune system to control and kill tumor by enhancing or restoring anti-tumor immunity. Tumor immunotherapy has shown to produce long-lasting responses in large numbers of patients, and thereby adoptive immunotherapy and immune checkpoint inhibitors could induce remarkable antigen-specific immune responses. Tumor infiltrating lymphocytes (TILs) are highly heterogeneous lymphocytes existing in tumor tissues and play a crucial role in host antigen-specific tumor immune response. Recent studies show that TILs are closely related to the prognosis of patients during the processes of tumorigenesis and treatment. Adoptive immunotherapy mediated by TILs has displayed favorable curative effect in many solid tumors. This paper reviews the recent progress of TILs in solid tumors.

Clinical significance of circulating tumor cells and their expression of cyclooxygenase-2 in patients with nasopharyngeal carcinoma.

To explore the expression of circulating tumor cells (CTCs) and cyclooxygenase-2 (COX-2) in CTCs and to assess their association with clinical parameters and treatment. Peripheral blood samples from 50 patients with nasopharyngeal carcinoma (NPC) were included. We applied advanced CanPatrolTM CTC enrichment technique and in situ hybridization assay to isolate, identify, and classify CTCs and COX-2 in CTCs. Epstein-Barr virus DNA was detected by Real Time-quantitative PCR (RT-qPCR). No CTCs were identified in ten healthy volunteers (100%). Of the total patients, 48 (96%) had positive CTCs counts and 36 (72%) had positive mesenchymal CTCs counts before treatment. CTCs cells were highly expressed in different NPC stages, and the positive ratio of mesenchymal CTCs in stage IV was higher than that in other stages. The proportion of mesenchymal cells was higher expressed in metastasis patients. The expression of COX-2 was different in different types of CTCs. The positivity of COX-2 in CTCs was higher in stage IV patients than that in stage II and stage III patients. Decreased mesenchymal CTCs and that express COX-2 indicated a favorable curative effect in NPC patients. The positivity of mesenchymal CTCs and COX-2 was higher in EBV DNA positive patients compared with EBV DNA negative patients (p<0.05). Meanwhile, the mean number of CTCs, mesenchymal CTCs, CTCs that express COX-2, hybrid CTCs that express COX-2 and mesenchymal CTCs that express COX-2 was significantly higher in the EBV DNA positive patients than negative patients before treatment (p<0.05). CTCs and their expression of COX-2 were correlated with NPC clinical characteristics, and have a relation with Epstein-Barr virus DNA. Decreased mesenchymal CTCs and that express COX-2 indicated a favorable curative effect in NPC patients.

Co-Delivery of Triptolide and Curcumin for Ovarian Cancer Targeting Therapy via mPEG-DPPE/CaP Nanoparticle.

Triptolide has proven to possess anticancer potential and been widely used for anti-cancer research. However, the liver and kidney toxicity has limited its application in cancer treatment. In this study, a drug delivery system based on mPEG-DPPE/calcium phosphate was developed to co-load triptolide and curcumin (TP and Curc-NPs). The coefficient of drug interaction (CDI) was calculated to determine the optimal concentration of the two drugs. When the concentration of triptolide was 25.22 ng/mL and the concentration of curcumin was 6.62 μg/mL, the two drugs reached the maximum synergistic killing effects on SKOV-3 tumor cells. The TP and Curc-NPs was prepared using ultrasonic emulsification. Flow cytometry results revealed that the TP and Curc-NPs arrested cell-cycle in the S and G2/M phases and exhibited a strong ability to induce apoptosis. Intracellular reactive oxygen species (ROS) results indicated that curcumin could reduce the intracellular ROS level caused by triptolide. The mRNA levels of heat shock protein (HSP) were detected by qTR-PCR and the results showed that the TP and Curc-NPs could lower the HSP70 mRNA level while could not reduce the HSP90 mRNA level. The animal experiments demonstrated the favorable curative effects of the TP and Curc-NPs, and the tumor inhibition rate reached 68.78%. The results of the pathological examinations demonstrated that the nanoparticles had no significant toxic effects on important organs. In conclusion, the TP and Curc-NPs exerted synergistic effects on ovarian cancer in vitro and in vivo, and the toxicity caused by triptolide may be reduced by curcumin through anti-oxidative stress effects. The TP and Curc-NPs could be a promising strategy for ovarian cancer.

A retrospective study of epidural and intravenous steroids after percutaneous endoscopic lumbar discectomy for large lumbar disc herniation

ObjectiveTo assess the early curative effect of epidural or intravenous administration of steroids during a percutaneous endoscopic lumbar discectomy (PELD). Methods28 consecutive patients who underwent PELD due to large lumbar disc herniation between November 2014 and January 2016 were followed up for 6 months. These patients were divided into two groups according to the treatment they received after PELD. 14 patients (Group A) were treated by PELD and epidural steroids, while the other 14 patients (Group B) were treated by PELD and intravenous steroids. We evaluated the effectiveness by the preoperative and postoperative visual analogue scale (VAS) scores for back and leg pain, and the postoperative Oswestry disability index (ODI) at 3 weeks after surgery via the clinical charts and telephone interview. Postoperative hospital stay and time return to work were investigated as well. ResultsThere is a significant decrease in VAS (back, leg), ODI, and time return to work (p < 0.05). For VAS (back), Group A showed a significant decrease compared with Group B at 1 day and 1 week after surgery (p = 0.011, p = 0.017). As for VAS (leg), Group A showed a significant decrease compared with Group B at 1 day, 1 week, 3 weeks, and 3 months follow-up examinations (p = 0.002, p = 0.006, p < 0.001, p < 0.001). For ODI, Group A showed a notable decrease compared with Group B (p < 0.001). The postoperative hospital stay in two groups was not statistically different (p = 0.636). But the time return to work in Group A was significantly shorter than that in Group B (p = 0.023). ConclusionPatients who underwent PELD with epidural steroid administration for large lumbar disc herniation showed favorable curative effect compared with those who underwent PELD with intravenous steroid administration.

JIEYUANSHEN DECOCTION EXERTS ANTIDEPRESSANT EFFECTS ON DEPRESSIVE RAT MODEL VIA REGULATING HPA AXIS AND THE LEVEL OF AMINO ACIDS NEUROTRANSMITTER.

Background:Jieyuanshen decoction (JYAS-D) - a traditional Chinese medicine was invented by Professor Nie based on classic formulas, chaihu jia longgu muli decoction has been proved as having favorable curative effects on depression in clinical practices. The aim of this study was to investigate the antidepressant effects and its molecular mechanism of JYAS-D.Materials and Methods:The model of depression was established by Chronic Unpredictable Stress. Different doses (8.2 g/kg, 16.3 g/kg, 32.7 g/kg) of JYAS-D was orally administered; Fluoxetine was orally administered with 10mg/kg. All treatments lasted for 28 days. Sucrose preference and open-field tests were adopted to observe the behavior of rats. OPA (ortho-phthalaldehyde) derivatization method was used to detect the contents of amino acid neurotransmitter. RIA (Radiation immunity analysis) method was used to measure the serum concentrations of CORT (Corticosterone), ACTH (Adrenocorticotropic hormone) and CRH (Corticotropin-releasing hormone). ELISA (Enzyme linked immunosorbent assay) method was adopted to examine the contents of Glucocorticoid receptor (GR) and Mineralocorticoid receptor (MR) in hippocampus.Results:Compared with the model group, sucrose preference was increased in all treatment groups. The concentration of serum CORT was reduced in the middle dose of JYAS-D and control groups; the concentration of serum ACTH was reduced in the low and high-dose of JYAS-D; the concentration of serum CRH was reduced in the middle and high-dose of JYAS-D. The content of hippocampus GR was increased in the middle and high-dose of JYAS-D; the content of hippocampus Glu (Glutamic acid) was reduced among the low, middle and high-dose of JYAS-D and fluoxetine group, the ratio of Glu/γ-GABA (y-aminobutyric acid was reduced in the low and high-dose of JYAS-D.Conclusion:JYAS-D had a significant antidepressant-like effect on rat model through regulating serum concentration of CORT, ACTH and CRH, increasing the content of hippocampus GR and regulating the equilibrium of amino acids neurotransmitter.

Distribution and clinical significance of circulating tumor cells in nasopharyngeal carcinoma.

Nasopharyngeal carcinomais one of the leading malignancies with obscure etiology. Circulating tumor cellshave been showed to intimately correlate with characteristics in different kinds of cancer. But links between circulating tumor cells andnasopharyngeal carcinoma were still lacking. Therefore, we exploredcirculating tumor cells' distribution innasopharyngeal carcinoma and their possible associations withnasopharyngeal carcinoma. Firstly, we found that the positive ratio ofcirculating tumor cells is extremely high in four stages ofnasopharyngeal carcinoma. Meanwhile, positive ratios of mesenchymalcirculating tumor cells were higher in early stages ofnasopharyngeal carcinoma. Apart from epithelialcirculating tumor cells, total, hybrid and mesenchymalcirculating tumor cells were correlated withnasopharyngeal carcinoma clinical stage. Our results showed that hybrid and mesenchymalcirculating tumor cells were associated withnasopharyngeal carcinoma metastasis (both distant and lymph node) and smoking. Meanwhile, hybridcirculating tumor cells expressed the highest Epstein-Barr virus proteins and deoxyribonucleic acid in three types ofcirculating tumor cells. Moreover, we found that Epstein-Barr virusproteins viral-caspid antigen-immunoglobulin A (VCA/IgA) and early antigen-immunoglobulin A (EA/IgA), but not Epstein-Barr virus-deoxyribonucleic acid, had a closed association withnasopharyngeal carcinoma metastasis. However,Epstein-Barr virus hallmarks failed to associate with othernasopharyngeal carcinoma characteristics. Furthermore, we confirmed that matrix metalloproteinase 9existed incirculating tumor cells and expressed most in mesenchymalcirculating tumor cells. In addition, matrix metalloproteinase 9-expressed extent in hybridcirculating tumor cells is somewhat different from epithelial and mesenchymalcirculating tumor cells in matrix metalloproteinase 9-positivecirculating tumor cells. Nevertheless, matrix metalloproteinase 9 had no relationship with othernasopharyngeal carcinoma characteristics. Finally, our results showed thatcirculating tumor cells were decreased in patients after therapies. Taken together,circulating tumor cells were tightly correlated withnasopharyngeal carcinoma characteristics. In addition, Epstein-Barr virus was associated withnasopharyngeal carcinoma metastasis. Of note, decreasedcirculating tumor cells indicated a favorable curative effect in nasopharyngeal carcinoma patients.

尼莫地平与羚角钩藤汤在治疗急性缺血性脑卒中的疗效观察

目的：回顾性总结2012年泉州市中医院内一科收治的急性缺血性脑卒中患者30例。方法：回顾性分析2012.01~2012.12全年泉州市中医院内一科收治的急性缺血性脑卒中患者30例，总结治疗方法。结果：本文观察的30例患者经采用尼莫地平配合羚角钩藤汤治疗治疗后基本痊愈20例，显著进步8例，进步1例，自动出院1例。结论：采用尼莫地平配合羚角钩藤汤治疗急性缺血性脑卒中痰热内闭证患者可取得良好疗效。 Objective: This paper’s purpose is to summarize retrospectively the illness cases of 30 patients who got the acute ischemic stroke and treated at the No. 1 Department of Internal Medicine, Quanzhou Hospital of Traditional Chinese Medicine in 2012. Methods: This thesis wants to find out the best treatment through the retrospective analysis towards the 30 cases of acute ischemic stroke treated in the No. 1 Department of Internal Medicine, Quanzhou Hospital of Traditional Chinese Medicine during the whole year of 2012. Results: The result is effective. There were twenty cured cases, eight markedly improved cases, one improved case and one case who left hospital initiatively. Conclusion: The analysis shows the conclusion that to treat the patient who gets Syndrome of Phlegm-heat Blocking Internally by combining Nimodipine with Cornus Antelopis and Uncariae Decoction will get favorable curative effect.

Clinical analysis of 5 eyes with corneal endotheliitis after cataract surgery

Objective To discuss the diagnosis and therapy of corneal endotheliitis after cataract surgery.Methods The clinical manifestation and treatment of 5 patients (5 eyes) of corneal endotheliitis after cataract surgery were analyzed retrospectively.All the patients were treated with antiviral medicine,glucocorticoid and IOP-droping medication.The follow up period was 4 - 9 months.Results 5 patients ( 5 eyes) were cured without menifestation of recurrence.Conclusion Enough glucocorticoid can release inflammation of endothelial cell and trabecular meshwork quickly,control the intraocular pressure and retrieve the physiologic function of corneal endothelium.The antiviral and IOP-droping medication can make patients get favorable curative effect. Key words: Cataract; Phacoemulsification; Corneal endotheliitis

Curative Effects of Oleanolic Acid on Formed Hypertrophic Scars in the Rabbit Ear Model

Hypertrophic scarring is a common proliferative disorder of dermal fibroblasts characterized by collagen overproduction and excessive deposition of extracellular matrix (ECM). There is no consensus about the best therapeutics to produce complete and permanent improvement of scars with few side effects. To investigate the therapeutic effects of oleanolic acid (OA) on hypertrophic scars and explore the possible mechanism of action involved, a rabbit ear model with hypertrophic scars was established. OA (2.5%, 5%, and 10%) was given once daily to the scars for 28 consecutive days. As a result, OA significantly alleviated formed hypertrophic scars on rabbit ears. The levels of TGF-β 1, MMP-1, TIMP-1, and collagens I and III were notably decreased, and the number of apoptosis cells and mRNA expression of MMP-2, caspase-3, and caspase-9 were markedly increased in the scar tissue. The scar elevation index (SEI) was also evidently reduced. Histological findings exhibited significant amelioration of the collagen tissue. These results suggest that OA has the favorable curative effects on formed hypertrophic scars in the rabbit ear model, and the possible mechanism of action is that OA decreases HSFs proliferation and increases HSFs apoptosis by reduction of P311 gene expression and TGF-β 1 production, inhibition of TIMP-1 secretion, enhancement of MMP-2 activity, and subsequently facilitation of degradation of collagen types I and III.

Studies on the application of ferrous Sulphate for controlling chlorosis of Apple tree on calcareous soils

Abstract Our large‐area field experiments show that a favorable curative effect of line induced chlorosis on apple trees was obtained with only 1–8 kilograms of ferrous sulphate applied to a large bearing tree. The most favorable effect on controlling chlorosis can be acquired by applying ferrous sulphate‐organic “mixed fertilizers in 8–10 holes along the crown of a tree. The mixed fertilizers giving good results were 1:10, 1:7 or 1:5 ferrous sulphate and horse manure, or cottonseed cake, wood dust, ammonium humate. The application of fertilizer in numerous positions is advantageous to the all‐sided renewal of diseased trees. Application before new leaf growth in spring was advantageous and helped to decrease toxicity. Organic matter also minimized toxicity.