This prospective study examined changes in parent–child relationship quality and well-being during the transition to university. We also investigated whether living situation (i.e., moving out of the parental home) and motivation to leave home were related to these changes. The participants were 240 Turkish university students (65.4% female; Mage = 17.74 (0.53) at Time 1) participating in two measurement waves from 2017 to 2019. Results of Latent Change Score Models revealed that both autonomy support of fathers and perceived stress decreased over time, while other parent–child relationship qualities and life satisfaction remain relatively stable on average. First-year university students who left home showed less decline in stress than those still living at home. We did not find living situation and motivation to leave home to moderate changes in parent–child relationship quality and well-being. Furthermore, we found bidirectional associations mainly between parent–child relationship quality and life satisfaction and between conflict with mother and stress. These findings underline the importance of considering the changes in parent–child relationship quality and well-being in the transition to university in a family-oriented culture.