Fat Production Research Articles

In vitro Insect Fat Cultivation for Cellular Agriculture Applications.

Cell-cultured fat could provide important elements of flavor, nutrition, and texture to enhance the quality and therefore expand consumer adoption of alternative meat products. In contrast to cells from livestock animals, insect cells have been proposed as a relatively low-cost and scalable platform for tissue engineering and muscle cell-derived cultured meat production. Furthermore, insect fat cells have long been cultured and characterized for basic biology and recombinant protein production but not for food production. To develop a food-relevant approach to insect fat cell cultivation and tissue engineering, Manduca sexta cells were cultured and induced to accumulate lipids in 2D and 3D formats within decellularized mycelium scaffolding. The resultant in vitro fat tissues were characterized and compared to in vivo fat tissue data by imaging, lipidomics, and texture analyses. The cells exhibited robust lipid accumulation when treated with a 0.1 mM soybean oil emulsion and had "healthier" fat profiles, as measured by the ratio of unsaturated to saturated fatty acids. Mycelium scaffolding provided a simple, food-grade approach to support the 3D cell cultures and lipid accumulation. This approach provides a low-cost, scalable, and nutritious method for cultured fat production.

The Role and Implications of Epicardial Fat in Coronary Atherosclerotic Disease

The current minireview aims to assess the implications of epicardial fat secretory function in the development of coronary artery disease. The epicardial adipose tissue (EAT) is a visceral fat depot that has been described as a cardiovascular risk factor. In addition to its mechanical protection role and physiological secretory function, it seems that various secretion products of the epicardial fat are responsible for metabolic disturbances at the level of the cardiac muscle when in association with pre-existing pathological conditions, such as metabolic syndrome. There is a pathological reduction in sarcomere shortening, abnormal cytosolic Ca2+ fluxes, reduced expression of sarcoplasmic endoplasmic reticulum ATPase 2a and decreased insulin-mediated Akt-Ser473-phosphorylation in association with abnormal levels of epicardial fat tissue. Activin A, angiopoietin-2, and CD14-positive monocytes selectively accumulate in the diseased myocardium, resulting in reduced cardiomyocyte contractile function. At the same time, it is believed that these alterations in secretory products directly decrease the myocyte function via molecular changes, thus contributing to the development of coronary disease when certain comorbidities are associated.

RNA-Seq and lipidomics reveal different adipogenic processes between bovine perirenal and intramuscular adipocytes

ABSTRACT Adipogenesis involves complex interactions between transcription and metabolic signalling. Exploration of the developmental characteristics of intramuscular adipocyte will provide targets for enhancing beef cattle marbling without increasing obesity. Few reports have compared bovine perirenal and intramuscular adipocyte transcriptomes using the combined analysis of transcriptomes and lipid metabolism to explore differences in adipogenic characteristics. We identified perirenal preadipocytes (PRA) and intramuscular preadipocytes (IMA) in Qinchuan cattle. We found that IMA were highly prolific in the early stages of adipogenesis, while PRA shows a stronger adipogenic ability in the terminal differentiation. Bovine perirenal and intramuscular adipocytes were detected through the combined analysis of the transcriptome and metabolome. More triglyceride was found to be upregulated in perirenal adipocytes; however, more types and amounts of unsaturated fatty acids were detected in intramuscular adipocytes, including eicosapentaenoic acid (20:5 n-3; EPA) and docosahexaenoic acid (22:6 n-3; DHA). Furthermore, differentially expressed genes in perirenal and intramuscular adipocytes were positively correlated with the eicosanoid, phosphatidylcholine (PC), phosphatidyl ethanolamine (PE), and sphingomyelin contents. Associated differential metabolic pathways included the glycerolipid and glycerophospholipid metabolisms. Our research findings provide a basis for the screening of key metabolic pathways or genes and metabolites involved in intramuscular fat production in cattle.

The effect of red dragon fruit (Hylocereus polyrhizus) peel meal on internal organs, ileal coliform, and growth performances in broiler chickens

The study evaluated the effect of feeding red dragon fruit (Hylocereus polyrhizus) peel meal on internal organs, ileal coliform counts, and growth performances in broiler chicken. A total of 96 birds of unsexed one-day-old broiler (Ross strain) were randomly divided into four dietary treatments and six replications with four birds each. The dietary treatments were basal diet as a control diet (DPM0), basal diet added with 0.5% red dragon fruit peel meal (DPM1), and 1% red dragon fruit peel meal (DPM2), and 1.5% red dragon fruit peel meal (DPM3). The cecum and carcass percentage in the broiler was significantly enhanced (p<0.05). At the same time, the coliform total in the ileum and abdominal fat were significantly decreased (p<0.05) with the increasing level of feeding dragon fruit peel meal. The difference in other internal organs' feed intake and body weight gain during the treatments were insignificant (p>0.05) after five weeks of feeding. In conclusion, the red dragon fruit peel meal effectively improves growth performance and gut health by increasing carcass percentage and decreasing abdominal fat production and ileal coliform count in broiler chickens.

Improvement of Adaptive Saanen Goat Milk Production and Reproduction Fed Diet Supplemented with Indigofera zollingeriana Leaf Meal

The objective of this research was to examine the effect of the addition of Indigofera zollingeriana leaf meal (IZLM) in diets on milk production and fermentation products of adaptive Saanen goat. The research was conducted at Balai Besar Pembibitan Ternak Unggul dan Hijauan Pakan Ternak (BBPTUHPT) or or the Center for Superior Animal Breeding and Forage Animal Feed, Baturraden, Central Java. A total of 18 first lactating adaptive Saanen goats with a body weight of 34.83±7.13 and aged 20-24 months were used in this study. The goats were kept in individual pens and grouped into 6 groups based on body weight and each group were randomized to receive three kinds of concentrate substitution with IZLM of 0%, 10% and 20 % of dry matter (DM) concentrates, for A, B and C treatments, respectively. Therefore, this research was designed according to randomized block design. Dry matter intake (DMI) of each goat was 4.5% of live weight with dry matter (DM) ratio of forage and concentrate were 60:40. The variables measured were DMI, partial volatile fatty acid (VFA), energy conversion efficiency of glucose into VFA (ECEVFA), methane gas, total protozoa, milk production and the first estrus after kidding. Analysis of variance showed that the treatment had a significant effect (P <0.05) on propionate, milk production and fat, but no significant effect (P> 0.05) on consumption of DM, acetate, butyrate, EKVFA, methane gas, lactose and protein milk. Increasing the IZLM substitution level enhanced linearly (P<0.05) on production of milk and milk fat, while the propionate concentrate responded to quadratic (P<0.05). Milk production increased at IZLM level of 20%, while goat in this group resulted only 16.70% estrous after birth compared to goat group received 10% IZLM level resulting 50% of estrus goat. The results of this study concluded that the recommended level of using IZLM as a concentrate substitute was only 10%.

Application of organogel‐like structured system as an alternative for reducing saturated fatty acid and replacing fat in milk ice cream

This work aimed to produce a healthier milk ice cream premium formulation (gelato), preserving the structural and indulgence characteristics replacing the milk fat cream by structured colloidal systems. The highest overrun values were observed in formulations produced with 50% fat reduction followed by a reduction of 100% and the Control with less air incorporation. The 100 % fat replacement by organogel formulation demonstrated the most prominent melt resistance and higher protein content. The rheological tests showed that the formulation with total fat reduction presented the biggest differences with better structuring. The complete substitution of fat by organogel makes the ice cream whiter. Applying organogel systems to premium ice cream (gelato), the total replacement of the milk fat was a successful approach to obtaining nutritious and low saturated fat products with added phytosterols and collagen for people who wish to improve the nutritional standard related to ice cream intake.

The health, cost and equity impacts of restrictions on the advertisement of high fat, salt and sugar products across the transport for London network: a health economic modelling study

BackgroundPolicies aimed at restricting the marketing of high fat, salt and sugar products have been proposed as one way of improving population diet and reducing obesity. In 2019, Transport for London implemented advertising restrictions on high fat, salt and sugar products. A controlled interrupted time-series analysis comparing London with a north of England control, suggested that the advertising restrictions had resulted in a reduction in household energy purchases. The aim of the study presented here was to estimate the health benefits, cost savings and equity impacts of the Transport for London policy using a health economic modelling approach, from an English National Health Service and personal social services perspective.MethodsA diabetes prevention microsimulation model was modified to incorporate the London population and Transport for London advertising intervention. Conversion of calorie to body mass index reduction was mediated through an approximation of a mathematical model estimating weight loss. Outcomes gathered included incremental obesity, long-term diabetes and cardiovascular disease events, quality-adjusted life years, healthcare costs saved and net monetary benefit. Slope index of inequality was calculated for proportion of people with obesity across socioeconomic groups to assess equity impacts.ResultsThe results show that the Transport for London policy was estimated to have resulted in 94,867 (4.8%) fewer individuals with obesity, and to reduce incidence of diabetes and cardiovascular disease by 2,857 and 1,915 cases respectively within three years post intervention. The policy would produce an estimated 16,394 additional quality-adjusted life-years and save £218 m in NHS and social care costs over the lifetime of the current population. Greater benefits (e.g. a 37% higher gain in quality-adjusted life-years) were expected to accrue to individuals from the most socioeconomically deprived groups compared to the least deprived.ConclusionsThis analysis suggests that there are considerable potential health and economic gains from restricting the advertisement of high fat, salt and sugar products. The population health and economic impacts of the Transport for London advertising restrictions are likely to have reduced health inequalities in London.

Production of butter paste with dihydroquercetin technology

The study proposes a technology for producing butter paste with dihydroquercetin. For the production of butter paste with dihydroquercetin, a method of converting high-fat cream has been chosen. Organoleptic, physico-chemical characteristics of the quality of raw materials have been studied. The technology of butter paste with dihydroquercetin was developed. Butter paste with dihydroquercetin was obtained as follows. The quality of raw milk and dihydroquercetin was assessed. Milk was separated, and the resulting cream was pasteurized. Further, high-fat cream was obtained by secondary separation, and their normalization was carried out. Then, dihydroquercetin was added to the normalized mixture according to the recipe. The normalized mixture was converted into butter paste and directed to thermostatting. Butter paste with dihydroquercetin is a low fat product with functional properties. The product is intended for systematic use by the population of all age groups.

Adipose-Derived Stem Cell-Enriched Lipotransfer Reverses Skin Sclerosis by Suppressing Dermal Inflammation.

Scleroderma is a chronic autoimmune disease with an incidence of 2.7 per 100,000 people. Traditional lipotransfer has been used to treat atrophic sclerotic skin. Enzymatically processed cell-assisted lipotransfer and mechanically processed stromal vascular fraction gel are fat products with abundant adipose-derived stem cells. This study assessed whether adipose-derived stem cell-enriched lipotransfer elicits superior therapeutic effects on scleroderma. Scleroderma was induced in nude mice by injections of bleomycin for 4 weeks. Human-derived Coleman fat, cell-assisted lipotransfer, or stromal vascular fraction gel (0.1 ml) was injected into sclerotic lesions. Histologic examinations, terminal deoxynucleotidyl transferase dUTP nick end labeling, and expression analyses of inflammatory factors in skin lesions and transferred fat were performed at 4 weeks after implantation. Dermal thickness was lower in the groups injected with Coleman fat (339.0 ± 19.66 µm), cell-assisted lipotransfer (271.0 ± 16.15 µm), and stromal vascular fraction gel (197.8 ± 12.99 µm) than in the group injected with phosphate-buffered saline (493.3 ± 28.13 µm) ( p < 0.05). The numbers of terminal deoxynucleotidyl transferase dUTP nick end labeling + and Mac2 + cells in fat tissue were significantly higher in the group injected with Coleman fat than in those injected with stromal vascular fraction gel and cell-assisted lipotransfer. Expression of monocyte chemotactic protein-1 and interleukin-6 was significantly lower in the adipose-derived stem cell-enriched groups than in the Coleman fat group. Histologic analysis showed there were far fewer macrophages and myofibroblasts in skin lesions in the adipose-derived stem cell-enriched groups than in the Coleman fat group. Transplantation of stromal vascular fraction gel and cell-assisted lipotransfer, which contain abundant adipose-derived stem cells, reduces the levels of apoptotic cells and inflammation, significantly reverses skin sclerosis, and elicits superior anti-inflammatory and antifibrotic effects on scleroderma. This study provided an alternative adipose-based therapy, adipose-derived stem cell-enriched fat, for sclerotic lesions and showed its validity for interfering with the inflammation and fibrosis.

Physicochemical and textural properties of natural cosmeceutical fats prepared from interesterified oil blends consisting of palm olein and palm kernel oil

Natural oil is commonly used as the base oil of high-end cosmetics. To meet the requirements of general users, the ratio of traditional vegetable oil-based interesterified fats continues to increase in industrial applications. Common interesterified fats are derived from chemical catalysis, which shows high specification requirements for raw materials and catalysts. Although enzymatic interesterification (EIE) is not commonly used in industry, it is far more economical, healthy and environmentally friendly. This study aims to use palm olein (POL) and lauric acid-based palm kernel oil (PKO) as raw materials to produce EIE fats by using Lipozyme TLIM® as the catalyst. The migration of acyl groups during EIE contributed to the modifications of the triacylglycerol compositions, the production of fats with fine crystals due to the accelerated crystallization rate, and improved fat properties such as plasticity, melting profile, viscosity, and spreadability. The interesterified cosmetic base oil showed reduced hydrophobicity and unaltered refractive index with no toxic heavy metal elements introduced during the process.

Assessing Amino Acid Metabolism in Splanchnic Tissues and Mammary Glands to Short-Term Graded Removal of Lys From an Abomasal-Infused Amino Acid Mixture in Lactating Goats.

To investigate the responses of amino acid metabolism in portal-drained viscera (PDV), liver, and mammary glands (MGs) to a graded gradual decrease of post-ruminal Lys supply, four multi-catheterized lactating goats were used in a 4 × 4 Latin square experiment. Goats were fasted for 12 h and then received a 33-h abomasal infusion of an amino acid mixture and glucose. Treatments consisted of a graded decrease of Lys content in the infusate to 100 (complete), 60, 30, or 0% as in casein. Lys-removed infusions decreased the production of milk, milk protein, fat, and lactose linearly and also decreased arterial Lys concentrations linearly (p < 0.05). Net PDV uptake decreased linearly (p < 0.05) with decreasing PDV loss ratio (p < 0.05). Although liver removal of Lys decreased linearly (p < 0.05), the removal ratio relative to portal absorption changed small, which was about 10% in all four treatments. Reduced Lys supply resulted in a linear decrease in the utilization of Lys in the peripheral tissues (except mammary, p < 0.05) and the release of more Lys in MGs. Although net mammary uptake of Lys declined linearly (p < 0.05), lactating goats can partially offset the negative effect of decreased circulating Lys concentrations by increasing mammary affinity (p < 0.05) and increasing mammary blood flow (p < 0.05). Graded removal of Lys from the infusate linearly decreased mammary uptake-to-output ratios of Lys (p < 0.05) suggesting that mammary catabolism of Lys decreased. Meanwhile, the treatments linearly increased circulating concentrations of glucagon and linearly decreased prolactin (p < 0.05). In conclusion, the results of the present study indicated that there were several mechanisms used to mitigate a Lys deficiency, including reduced catabolism of Lys in PDV and peripheral tissues (including MGs) and linearly increased mammary blood flow and mammary affinity together with increased mammary uptake and U:O of branched-chain amino acids (BCAA). Given these changes, the decline in milk protein production could be attributed to the combined effect of mass action with Lys and hormonal status.

MRCKα is a novel regulator of prolactin-induced lactogenesis in bovine mammary epithelial cells.

Myotonic dystrophy-related Cdc42-binding kinase alpha (MRCKα) is an integral component of signaling pathways controlling vital cellular processes, including cytoskeletal reorganization, cell proliferation and cell survival. In this study, we investigated the physiological role of MRCKα in milk protein and fat production in dairy cows, which requires a dynamic and strict organization of the cytoskeletal network in bovine mammary epithelial cells (BMEC). Within a selection of 9 Holstein cows, we found that both mRNA and protein expression of MRCKα in the mammary gland were upregulated during lactation and correlated positively (r > 0.89) with the mRNA and protein levels of β-casein. Similar positive correlations (r > 0.79) were found in a primary culture of BMEC stimulated with prolactin for 24 h. In these cells, silencing of MRCKα decreased basal β-casein, sterol-regulatory element binding protein (SREBP)-1 and cyclin D1 protein level, phosphorylation of mTOR, triglyceride secretion, cell number and viability—while overexpression of MRCKα displayed the reversed effect. Notably, silencing of MRCKα completely prevented the stimulatory action of prolactin on the same parameters. These data demonstrate that MRCKα is a critical mediator of prolactin-induced lactogenesis via stimulation of the mTOR/SREBP1/cyclin D1 signaling pathway.

Seasonal and sex-dependent gene expression in emu (Dromaius novaehollandiae) fat tissues.

Emu (Dromaius novaehollandiae) farming has been gaining wide interest for fat production. Oil rendered from this large flightless bird’s fat is valued for its anti-inflammatory and antioxidant properties for uses in therapeutics and cosmetics. We analyzed the seasonal and sex-dependent differentially expressed (DE) genes involved in fat metabolism in emus. Samples were taken from back and abdominal fat tissues of a single set of four male and four female emus in April, June, and November for RNA-sequencing. We found 100 DE genes (47 seasonally in males; 34 seasonally in females; 19 between sexes). Seasonally DE genes with significant difference between the sexes in gene ontology terms suggested integrin beta chain-2 (ITGB2) influences fat changes, in concordance with earlier studies. Six seasonally DE genes functioned in more than two enriched pathways (two female: angiopoietin-like 4 (ANGPTL4) and lipoprotein lipase (LPL); four male: lumican (LUM), osteoglycin (OGN), aldolase B (ALDOB), and solute carrier family 37 member 2 (SLC37A2)). Two sexually DE genes, follicle stimulating hormone receptor (FSHR) and perilipin 2 (PLIN2), had functional investigations supporting their influence on fat gain and loss. The results suggested these nine genes influence fat metabolism and deposition in emus.

Effects of Vitamin A on Yanbian Yellow Cattle and Their Preadipocytes by Activating AKT/mTOR Signaling Pathway and Intestinal Microflora.

Simple SummaryVitamin A is a fat-soluble vitamin that not only plays a role in vision, growth, and development, but also in fat production and metabolism in animals. To improve the production of high-grade beef, it is necessary to explore the molecular mechanism of intramuscular fat deposition in beef cattle through molecular biology techniques. In this study, we selected Yanbian yellow cattle, one of the five major cattle breeds in China, to investigate the effects of vitamin A and its metabolite, all-trans retinoic acid (ATRA), on the proliferation and differentiation of preadipocytes and changes in intestinal microorganisms. It was found that ATRA inhibited adipogenic differentiation of preadipocytes in Yanbian yellow cattle via the AKT/mTOR signaling pathway. This study provides insight into nutritional management and reveals the role of vitamin A in lipid metabolism in Yanbian yellow cattle.In this study, the effects of vitamin A and its metabolite, all-trans retinoic acid (ATRA), on the proliferation and differentiation of preadipocytes and the intestinal microbiome in Yanbian yellow cattle were investigated. Preadipocytes collected from Yanbian yellow cattle treated with different concentrations of ATRA remained in the G1/G0 phase, as determined by flow cytometry. Quantitative reverse-transcription polymerase chain reaction and western blotting analyses showed that the mRNA and protein expression levels of key adipogenic factors, peroxisome proliferator- activated receptor gamma (PPARγ), CCAAT enhancer-binding protein α (C/EBPα), and extracellular signal-regulated kinase 2 (ERK2), decreased. ATRA was found to regulate the mTOR signaling pathway, which is involved in lipid metabolism, by inhibiting the expression of AKT2 and the adipogenic transcription factors SREBP1, ACC, and FAS; the protein and mRNA expression levels showed consistent trends. In addition, 16S rRNA sequencing results showed that a low concentration of vitamin A promoted the growth of intestinal microflora beneficial to lipid metabolism and maintained intestinal health. The results indicated that ATRA inhibited the adipogenic differentiation of preadipocytes from Yanbian yellow cattle through the AKT/mTOR signaling pathway, and that low concentrations of vitamin A may help maintain the intestinal microbes involved in lipid metabolism in cattle.

Molecular Approaches Reduce Saturates and Eliminate trans Fats in Food Oils.

Vegetable oils composed of triacylglycerols (TAG) are a major source of calories in human diets. However, the fatty acid compositions of these oils are not ideal for human nutrition and the needs of the food industry. Saturated fatty acids contribute to health problems, while polyunsaturated fatty acids (PUFA) can become rancid upon storage or processing. In this review, we first summarize the pathways of fatty acid metabolism and TAG synthesis and detail the problems with the oil compositions of major crops. Then we describe how transgenic expression of desaturases and downregulation of the plastid FatB thioesterase have provided the means to lower oil saturates. The traditional solution to PUFA rancidity uses industrial chemistry to reduce PUFA content by partial hydrogenation, but this results in the production of trans fats that are even more unhealthy than saturated fats. We detail the discoveries in the biochemistry and molecular genetics of oil synthesis that provided the knowledge and tools to lower oil PUFA content by blocking their synthesis during seed development. Finally, we describe the successes in breeding and biotechnology that are giving us new, high-oleic, low PUFA varieties of soybean, canola and other oilseed crops.

Caffeic acid phenethyl ester suppresses intestinal FXR signaling and ameliorates nonalcoholic fatty liver disease by inhibiting bacterial bile salt hydrolase activity.

Propolis is commonly used in traditional Chinese medicine. Studies have demonstrated the therapeutic effects of propolis extracts and its major bioactive compound caffeic acid phenethyl ester (CAPE) on obesity and diabetes. Herein, CAPE was found to have pharmacological activity against nonalcoholic fatty liver disease (NAFLD) in diet-induced obese mice. CAPE, previously reported as an inhibitor of bacterial bile salt hydrolase (BSH), inhibited BSH enzymatic activity in the gut microbiota when administered to mice. Upon BSH inhibition by CAPE, levels of tauro-β-muricholic acid were increased in theintestine and selectively suppressed intestinal farnesoid X receptor (FXR) signaling. This resulted in lowering of the ceramides in theintestine that resulted from increased diet-induced obesity. Elevated intestinal ceramides are transported to the liver where they promoted fat production. Lowering FXR signaling was also accompanied by increased GLP-1 secretion. In support of this pathway, the therapeutic effects of CAPE on NAFLD were absent in intestinal FXR-deficient mice, and supplementation of mice with C16-ceramide significantly exacerbated hepatic steatosis. Treatment of mice with an antibiotic cocktail to deplete BSH-producing bacteria also abrogated the therapeutic activity of CAPE against NAFLD. These findings demonstrate that CAPE ameliorates obesity-related steatosis at least partly through the gut microbiota-bile acid-FXR pathway via inhibiting bacterial BSH activity and suggests that propolis enriched with CAPE might serve as a promising therapeutic agent for the treatment of NAFLD.

Third-Trimester Maternal Dietary Patterns Are Associated with Sleep Health among Adolescent Offspring in a Mexico City Cohort

BackgroundMaternal diet during gestation has been linked to infant sleep; whether associations persist through adolescence is unknown. ObjectivesWe explored associations between trimester-specific maternal diet patterns and measures of sleep health among adolescent offspring in a Mexico City birth cohort. MethodsData from 310 mother–adolescent dyads were analyzed. Maternal diet patterns were identified by principal component analysis derived from FFQs collected during each trimester of pregnancy. Sleep duration, midpoint, and fragmentation were obtained from 7-d actigraphy data when adolescents were between 12 and 20 y old. Unstratified and sex-stratified association analyses were conducted using linear regression models, adjusted for potential confounders. ResultsMean ± SD age of offspring was 15.1 ± 1.9 y, and 52.3% of the sample was female. Three diet patterns were identified during each trimester of pregnancy: the Prudent Diet (PD), high in lean proteins and vegetables; the Transitioning Mexican Diet (TMD), high in westernized foods; and the High Meat & Fat Diet (HMFD), high in meats and fat products. Mean ± SD sleep duration was 8.5 ± 1.5 h/night. Most associations were found in the third trimester. Specifically, PD maternal adherence was associated with shorter sleep duration among offspring (−0.57 h; 95% CI: −0.98, −0.16 h, in the highest tertile compared with the lowest) and earlier sleep midpoint among females (−0.77 h; 95% CI: −1.3, −0.26 h). Adherence to the HMFD and TMD was nonlinearly associated with less fragmented sleep, with the latter only evident among females. ConclusionsFindings indicate that maternal dietary patterns, especially during the third trimester of pregnancy, may have long-term impacts on offspring sleep.

Lipid Metabolism and Cancer.

Lipid metabolism is involved in the regulation of numerous cellular processes, such as cell growth, proliferation, differentiation, survival, apoptosis, inflammation, movement, membrane homeostasis, chemotherapy response, and drug resistance. Reprogramming of lipid metabolism is a typical feature of malignant tumors. In a variety of cancers, fat uptake, storage and fat production are up-regulated, which in turn promotes the rapid growth, invasion, and migration of tumors. This paper systematically summarizes the key signal transduction pathways and molecules of lipid metabolism regulating tumors, and the role of lipid metabolism in programmed cell death. In conclusion, understanding the potential molecular mechanism of lipid metabolism and the functions of different lipid molecules may facilitate elucidating the mechanisms underlying the occurrence of cancer in order to discover new potential targets for the development of effective antitumor drugs.

Frankincense-like Flavor Formation Through the Combined Effect of Moderate Enzymatically Hydrolyzed Milk Fat and Glutamic Acid-galactose Amadori Rearrangement Product During Thermal Processing

Frankincense-like Flavor Formation Through the Combined Effect of Moderate Enzymatically Hydrolyzed Milk Fat and Glutamic Acid-galactose Amadori Rearrangement Product During Thermal Processing

The relationship between dry period length and milk production of Holstein dairy cows in tropical climate: a machine learning approach.

The objective of this retrospective longitudinal study was to evaluate the relationship between dry period length and the production of milk, fat, protein, lactose and total milk solids in the subsequent lactation of Holstein dairy cows under tropical climate. After handling and cleaning of the data provided by the Holstein Cattle Breeders Association of Minas Gerais, data from 32 867 complete lactations of 19 535 Holstein animals that calved between 1993 and 2017 in 122 dairy herds located in Minas Gerais state (Brazil) were analysed. In addition to dry period length, calving age, lactation length, milking frequency, parity, calf status at birth, herd, year, and season of calving were included in the analysis as covariables to account for additional sources of variation. The machine learning algorithms gradient boosting machine, extreme gradient boosting machine, random forest and artificial neural network were used to train models using cross validation. The best model was selected based on four error metrics and used to evaluate the variable importance, the interaction strength between dry period length and the other variables, and to generate partial dependency plots. Random forest was the best model for all production outcomes evaluated. Dry period length was the third most important variable in predicting milk production and its components. No strong interactions were observed between the dry period and the other evaluated variables. The highest milk and lactose productions were observed with a 50-d long dry period, while fat, protein, and total milk solids were the highest with dry period lengths of 38, 38, and 44 d, respectively. Overall, dry period length is associated with the production of milk and its components in the subsequent lactation of Holstein cows under tropical climatic conditions, but the optimum length depends on the production outcome.