Fat Production Research Articles

Influence of the age of the first calving on some indicators of productive qualities of brown swiss cows in the conditions of the industrial complex

Presented the results of the research of the influence of different ages of the first insemination and the first calving of Brown Swiss cows on the realization of productive potential and reproductive qualities in a large industrial complex. Researches during 2019–2021 were conducted on the first-heifers of cows (n=242 cows) on the dairy industrial complex "Ekaterinoslavsky" of Dnipropetrovsk region. According to the age of the first calving, were formed five groups of first-heifers: group I (n=27) first calved at the age of 22.7 months, and fertilized – at the age of 13.4 months; group II (n=80) were first fertilized and calved at the age of 16.3 and 25.5 months, respectively; group III (n=56) – 18.9 and 28.1 months, respectively; IV group (n=38) – respectively 22,1 and 31.3 months; V group n=41 – respectively 26.5 and 35.8 months. The age of the first calving of experimental cows of all five groups differed at the level of P<0.001.
 It was found that the level of average daily milk yield of Swiss first-heifers of different ages at the first calving has a significant intergroup difference. Cows of II and III groups are characterized by high and almost identical indicators, in which they are 30.0 and 30.3 kg, respectively. Slightly lower average daily milk yield is observed in Swiss animals of IV group, in which it does not exceed 29.0 kg, which was inferior to the first-heifers of II group by 3.45 %, and animals of III group – by 4.29 %. Relatively the lowest average daily milk yield is characterized by the first-heifers of I group, in which it does not exceed 28.8 kg, which practically was equal to the milk yield of IV group of Swiss animals and was less than cows of II and III group by 4.17 and 5.21 %, respectively. The highest level of average daily milk yield in first-heifers of I group, in which it did not fall below 32.2 kg. This figure exceeds the animals of II and III groups by 6.83 % (P<0.05) and 5.90 % (P<0.05), respectively. In comparison with the first-heifers of IV and V groups, animals of I group have higher milk yields per day by 9.94 % (P<0.01) and 10.56 % (P<0.01), respectively.
 It was found that in the researches, the mass fraction of milk fat of all groups of first-heifers was close and ranged from normal to 3.93 % to 4.07 %, and protein – an average of 3.31–3.53 %. The ratio of fat and protein averaged 1.16-1.20, which is normal.
 It was found that the highest rate of milk yield during 305 days of lactation have first-heifers of the first group, who calved at the age of 22.7 months and have a milk yield of 9810.4 kg. At the same time, the milk productivity cows of II group, which calved at the age of 25.5 months, averaged 9140.0 kg, which is 7.33 % less than I group cows (P<0.05). In the first-heifers of III group, which calved for the first time at the age of 28.1 months, the level of milk productivity averages 9251.8 kg of milk, which is inferior to the first-heifers of I group by 6.04 % (P<0.05). Swiss first-heifers of IV group, in which the first calving was at the age of 31.3 months, have a milk yield of 8832.2 kg of milk, which is 11.08 % less than the rate cows of I group (P<0.01). Relatively the lowest level of milk productivity during 305 days of lactation is characterized by first-heifers of V group, in which the age of the first calving averaged 35.8 months. Only 8789.8 kg of milk was obtained from these animals, which is 12.32 % less than the first-heifers of I group (P<0.01). The total production of milk fat and protein in the first-heifers of I group is the highest and averages 711.3 kg, which is 7.0 % more than animals of II group (P<0.05).
 It is investigated that Swiss first-heifers of different ages of the first calving are characterized by satisfactory indicators of reproductive function, which are associated with a high level of milk productivity and do not depend on the age of the first calving. Compared to the biological norm in these cows, the service period is 1.76–2.24 times longer, the lactation period is 1.28–1.42 times longer, and the period between calving is 1,19–1,30 times. The adaptation index in all groups of first-heifers has a small negative value, which ranged from ˗5.89 to ˗8.23 units, which indicates satisfactory adaptive qualities in the conditions of intensive technology.

Production of bovine beta-lactoglobulin and hen egg ovalbumin by Trichoderma reesei using precision fermentation technology and testing of their techno-functional properties

The food protein ingredient market is dominated by dairy and egg proteins. Both milk whey and egg proteins are challenging proteins to replace, e.g. with plant proteins, due to the unique structural features of the animal proteins that render them highly functional. Thus, to provide a non-animal source of these important proteins the fungal host Trichoderma reesei was utilized for the biotechnical production of recombinant hen ovalbumin (TrOVA) and bovine beta lactoglobulin (TrBLG). These food proteins were investigated using two different promoter systems to test the concept of effectively expressing them in a fungal host. Both proteins were successfully produced in 24 well plate and bioreactor scale. The production level of TrBLG and TrOVA were 1 g/L and 2 g/L, respectively. Both proteins were further purified and characterized, and their functional properties were tested. TrBLG and TrOVA secondary structures determined by circular dichroism corresponded to the proteins of bovine and hen. The T. reesei produced proteins were found to be N-glycosylated, mostly with Man 5. TrBLG had emulsification properties matching to corresponding bovine protein. TrOVA showed excellent foaming characteristics and heat-induced gelation, although the strength of the gel was somewhat lower than with hen ovalbumin, possibly due to the partial degradation of TrOVA or presence of other host proteins. Biotechnical production of whey and egg proteins using precision fermentation technology offers an innovative way to increase the sustainability of the conventional food industry, without further reliance on animal farming.Industrial relevance: The food protein ingredient market is dominated by dairy (largely whey proteins) and egg proteins. Whey proteins are valuable and versatile food ingredients due to their functional and nutritional quality. They are largely used in meat and milk products, low fat products, bakery, confectionary, infant formulas and sports nutrition. Similarly, egg white protein ovalbumin is a highly functional protein ingredient that facilitates structure formation and high nutritional quality in most food products. Together they comprise 40–70% of the revenue in the animal protein ingredients market. Both whey and egg proteins are extremely challenging proteins to replace, e.g., by plant proteins due to their unique structural features that render them with high functionality. Biotechnical production of whey and egg proteins using precision fermentation technology offers an innovative way to increase the sustainability of the conventional food industry, without further reliance on animal farming.

Myoblast-derived exosomes promote the repair and regeneration of injured skeletal muscle in mice.

When skeletal muscle is damaged, satellite cells (SCs) are activated to proliferate rapidly and fuse with the damaged muscle fibers to form new muscle fibers, thereby promoting muscle growth and remodeling and repair of trauma. Exosomes from differentiating human skeletal muscle cells trigger myogenesis of stem cells and provide biochemical cues for skeletal muscle regeneration. Therefore, we hypothesized that, when muscles are injured, myoblast-derived exosomes may regulate muscle repair and regeneration. Here, we investigated the underlying mechanism by applying C2C12-derived exosomes to injured mouse skeletal muscles. The expression levels of skeletal muscle regeneration factors paired box 7 and lipid-promoting factor peroxisome proliferator-activated receptor γ were upregulated, whereas the expression levels of fibrosis factors collagen-1 and α-smooth muscle actin decreased. The expression of proliferating cell nuclear antigen was elevated after applying C2C12-derived exosomes to SCs. Application of C2C12-derived exosomes to fibro-adipogenic progenitors resulted in an increase in peroxisome proliferator-activated receptor γ expression and adipogenesis capacity, whereas α-smooth muscle actin expression and fibrosis capacity decreased. Analysis of the transcriptome and proteome of SCs after treatment with exosomes showed the involvement of multiple biological processes, including proliferation and differentiation of SCs, muscle regeneration, skeletal muscle atrophy, and the inflammatory response after muscle injury. Hence, our data suggest that C2C12-derived exosomes can promote the regeneration of skeletal muscle fibers, accelerate the production of fat from damaged muscles, inhibit the fibrosis of damaged muscles, and accelerate injury repair, which is related to exosome-mediated regulation of the proliferation of SCs, differentiation of fibro-adipogenic progenitors, and modulation of SC mRNA expression and protein formation and decomposition.

Comparison of 3-breed rotational crossbreds of Montbéliarde, Viking Red, and Holstein with Holstein cows fed 2 alternative diets for dry matter intake, production, and residual feed intake.

The objective of this study was to compare 3-breed rotational crossbred (CB) cows of the Montbéliarde, Viking Red, and Holstein (HO) breeds with HO cows fed 2 alternative diets for dry matter intake (DMI), fat plus protein production (CFP), body weight (BW), body condition score (BCS), feed efficiency, and residual feed intake (RFI) from 46 to 150 days in milk (DIM) during first lactation. The CB cows (n = 17) and HO cows (n = 19) calved from September 2019 to March 2020. Cows were fed either a traditional total mixed ration diet (TRAD) or a higher fiber, lower starch total mixed ration diet (HFLS). The HFLS had 21% more corn silage, 47% more alfalfa hay, 44% less corn grain, and 43% less corn gluten feed than the TRAD. The 2 diets were analyzed for dry matter content, crude protein, forage digestibility, starch, and net energy for lactation. The BW and BCS were recorded once weekly. Daily milk, fat, and protein production were estimated from twice monthly milk recording with random regression. Measures of efficiency were CFP per kilogram of DMI and DMI per kilogram of BW. The RFI from 46 to 150 DIM was the residual error from regression of DMI on milk energy, metabolic BW, and the energy required for change in BW. Statistical analysis of all variables included the fixed effects of diet, breed group, and the interaction of diet and breed group. The CB cows fed HFLS had less DMI (-12%) and lower DMI/BW (-14%) compared with the HO cows fed TRAD. For CFP, CB and HO cows were not different when fed TRAD or HFLS. Furthermore, the CB cows fed HFLS had higher BW (+50 kg) compared with HO cows fed HFLS. The CB cows fed TRAD had higher BCS than HO cows fed TRAD and HO cows fed HFLS (+0.46 and +0.62, respectively). The HO cows fed TRAD had more DMI (+14%) and lower CFP per kilogram of DMI (-12%) compared with the HO cows fed HFLS. In addition, mean RFI from 46 to 150 DIM was lower and more desirable for CB cows fed HFLS (-120.0 kg) compared with HO cows fed TRAD (85.3 kg). Dairy producers may feed either TRAD or HFLS to CB cows without loss of CFP.

Interaction of sodium acetate supplementation and dietary fiber level on feeding behavior, digestibility, milk synthesis, and plasma metabolites.

Acetate supplementation has been shown to increase milk fat yield in diets with low risk of biohydrogenation-induced milk fat depression. The interaction of acetate supplementation with specific dietary factors that modify rumen fermentation and short-chain fatty acid (FA) synthesis has not been investigated. The objective of this experiment was to determine the effect of acetate supplemented as sodium acetate at 2 dietary fiber levels. Our hypothesis was that acetate would increase milk fat production more in animals fed the low-fiber diet. Twelve lactating multiparous Holstein cows were arranged in a 4 × 4 Latin square design balanced for carryover effects with a 2 × 2 factorial arrangement of dietary fiber level and acetate supplementation with 21-d experimental periods. The high-fiber diet had 32% neutral detergent fiber and 21.8% starch, and the low-fiber diet had 29.5% neutral detergent fiber and 28.7% starch created by substitution of forages predominantly for ground corn grain. Acetate was supplemented in the diet at an average 2.8% of dry matter (DM) to provide approximately 10 mol/d of acetate as anhydrous sodium acetate. Acetate supplementation increased DM intake by 6%, with no effect on meal frequency or size. Furthermore, acetate supplementation slightly increased total-tract apparent DM digestibility and tended to increase organic matter digestibility. Acetate supplementation increased milk fat concentration and yield by 8.6 and 10.5%, respectively, but there was no interaction with dietary fiber. The increase in milk fat synthesis was associated with 46 and 85 g/d increases in the yield of de novo (<16C) and mixed source (16C) FA, respectively, with no changes in yield of preformed FA (>16C). There was a 9% increase in the concentration of milk mixed-source FA and a 7% decrease in milk preformed FA with acetate supplementation, regardless of dietary fiber level. Acetate supplementation also increased the concentrations of plasma acetate and β-hydroxybutyrate, major metabolic substrates for mammary lipogenesis. Overall, acetate supplementation increased milk fat yield regardless of dietary fiber level through an increase mostly caused by an increase in longer-chain de novo FA, suggesting stimulation of mammary lipogenesis. The heightened mammary de novo lipogenesis was supported by an increase in the concentration of metabolic substrates in plasma.

Provision Of Beer Dregs and Rejected Bread On Production and Milk Fat In Jabung Agro Trade Cooperatives Malang Regency

Background: In the maintenance of dairy cows, feed must be considered because it can affect the productivity of dairy cows, including milk production and quality. Purpose: to determine the effect of giving beer dregs and rejected bread on milk and milk fat production. Methods: The method used is primary data collection, recording milk and milk fat production for two weeks to determine the difference between giving beer dregs and rejected bread to milk and milk fat production. Results: The results showed that providing beer dregs and rejected bread to dairy cows could increase milk production and fat content. Cows as control treatment had fat content with an average of 3.95%, and cows given beer dregs had fat content with an average of 4.61%. Cows given rejected bread had fat content with an average of 5.01%. For the milk production of dairy cows as a control treatment, an average of 11.8 liters/day, the average production of dairy cows fed with beer dregs is 13.3 liters/day, and the production of dairy cows given rejected bread is an average of 14 liters/day. Conclusions: The conclusion that can be obtained is that the provision of beer dregs and rejected bread as additional feed has a significant effect on increasing milk production and milk fat content of dairy cows.

New approach combining food value with nutrient budgeting provides insights into the value of alternative farming systems

AbstractSustainable farming systems provide food for humans while balancing nutrient management. Inclusion or exclusion of livestock has nutrient management implications, as livestock produce food from otherwise inedible crops and their manure is a valuable soil conditioner. However, plant‐based diets are becoming more widespread due to perceived environmental benefits. We measure both food production in terms of nourishment to humans (in this study measured by protein, fat, starch and sugar production) and nutrient sustainability in terms of fertiliser use of six rotational farming systems with differences in nutrient management approaches. The arable practices included were the application of synthetic fertilisers, a range of organic amendments, incorporation of crop residues and legume cultivation. Livestock and associated products were included in some systems but excluded in others. The production of protein, fat, starch and sugar was combined with the balance of nitrogen (N), phosphorus (P) and potassium (K) into an overall measure of nutrient use efficiency of human macronutrient production. Across all systems considered, N use efficiency (5–13 kg protein/kg applied N) was lower than P (84–772 kg protein/kg applied P) or K (63–2060 kg protein/kg applied K), and combining synthetic fertiliser use with organic amendment applications raised production significantly while balancing P and K management, regardless of which organic amendment was used. Legume‐supported rotations without livestock produced more protein, starch and sugar per unit area than those with livestock. Nutrient balances and nutrient use efficiencies were more sensitive to management changes than purely food production. Using this approach allowed us to identify areas for improvement in food production based on the specific nutritional value of offtakes as opposed to yield overall.

Transcranial Direct-Current Stimulation Regulates MCT1-PPA-PTEN-LONP1 Signaling to Confer Neuroprotection After Rat Cerebral Ischemia–Reperfusion Injury

Propionic acid (PPA) is a critical metabolite involved in microbial fermentation, which functions to reduce fat production, inhibit inflammation, and reduce serum cholesterol levels. The role of PPA in the context of cerebral ischemia–reperfusion (I/R) injury has yet to be clarified. Increasing evidence indicate that transcranial direct-current stimulation (tDCS) is a safe approach that confers neuroprotection in cerebral ischemia injury. Here, we show that the levels of PPA were reduced in the ischemic brain following a rat cerebral I/R injury and in the cultured rat cortical neurons after oxygen–glucose deprivation (OGD), an in vitro model of ischemic injury. We found that the decreased levels of transporter protein monocarboxylate transporter-1 (MCT1) were responsible for the OGD-induced reduction of PPA. Supplementing PPA reduced ischemia-induced neuronal death after I/R. Moreover, our results revealed that the neuroprotective effect of PPA is mediated through downregulation of phosphatase PTEN and subsequent upregulation of Lon protease 1 (LONP1). We demonstrated that direct-current stimulation (DCS) increased MCT1 expression and PPA level in OGD-insulted neurons, while tDCS decreased the brain infarct volume in the MCAO rats via increasing the levels of MCT1 expression and PPA. This study supports a potential application of tDCS in ischemic stroke.

Effect of arginine supplementation on the production of milk fat in dairy cows.

Arginine, one of the conditionally essential AA, has been reported to affect fat synthesis and metabolism in nonruminant animals by influencing adenosine monophosphate activated protein kinase (AMPK) in some organs. In dairy cows, the effect of Arg on milk fat production is not clear, and any potential mechanism that underlies the effect is unknown. We tested the hypothesis that Arg infusion would improve the production of milk fat, and explored possible mechanism that might underlie any effect. We used 6 healthy lactating cows at 20 ± 2 d in milk, in fourth parity, with a body weight of 508 ± 14 kg, body condition score of 3.0 ± 0, and a milk yield of 30.6 ± 1.8 kg/d (mean ± standard deviation). The cows were blocked by days in milk and milk yield and each cow received 3 treatments in a replicated 3 × 3 Latin square design, with each of the experimental periods lasting 7 d with a 14-d washout between each period. The treatments, delivered in random order, were (1) infusion of saline (control); (2) infusion of 0.216 mol/d of l-Arg in saline (Arg); (3) infusion of 0.868 mol/d of l-Ala in saline (the Arg and Ala treatments were iso-nitrogenous) through a jugular vein. On the last day of each experimental period, blood was sampled to measure insulin, nitric oxide, glucose, and nonesterified fatty acid, and the liver and mammary gland were biopsied to measure the expression of genes. Milk yield was recorded, and milk fat percentage was measured daily during each of the experimental periods. The yield and composition of fatty acid (FA) in milk was measured daily on the last 3 d during each of the experimental periods. The data were analyzed using a mixed model with treatment as a fixed factor, and cow, period, and block as random factors. The daily milk yield and milk fat yield when the cows were infused with Arg were 2.2 kg and 76 g, respectively, higher than that in control, and 1.8 kg and 111 g, respectively, higher than that in Ala. When the cows were infused with Arg they had higher concentration and yield of de novo synthesized FA, than when they received the control or Ala infusions, although milk fat percentage, daily feed intake, and the digestibility of nutrients were not affected by treatment. The serum concentration of nitric oxide and insulin were higher during Arg than during control or Ala, with no difference between control and Ala. In the liver, the expression of the genes coding for AMPK (PRKAA1, PRKAB1, and PRKAG1) and genes related to the oxidation of FA were higher during Arg than during control or Ala, whereas in the mammary gland the expression PRKAB1 was lowest, and the expression of genes involved in the synthesis of milk fat were highest, during Arg infusion. The results suggest the intravenous infusion of Arg enhanced the production of milk fat by promoting the de novo synthesis of FA and increasing milk yield.

The impact of using different ancestral reference populations in assessing crossbred population admixture and influence on performance.

Crossbreeding is a process in which animals from different breeds are mated together. The animals produced will exhibit a combination of both additive and non-additive genetic improvement from parental breeds that increase heterozygosity and negate inbreeding depression. However, crossbreeding may also break up the unique and often beneficial gene combinations in parental breeds, possibly reducing performance potential as the benefits of heterosis depends on the type of crossbreeding systems used and heritability of the traits. This effect of crossbreeding, especially on the genome architecture, is still poorly understood with respect to 3-breed crossbreeding systems. Thus, this study examined variation in genomic ancestry estimations relative to pedigree-based estimations and correlated breed composition to key production and health traits. Two rotational crossbred populations, referenced as ProCROSS and Grazecross were assessed and totaled 607 crossbred cattle. ProCROSS is a product of rotational crossbreeding of Viking Red (VKR), Holstein (HOL), and Montbeliarde (MON). In contrast, Grazecross consists of Viking Red (VKR), Normande (NOR), and Jersey (JER). Both breeding programs were aimed at capitalizing on the positive effect of heterosis. The VKR is a marketing term for Swedish Red, Danish Red, and Finnish Ayrshire breed which complicated breed determination. Therefore, genomic breed composition estimates were compared using two different representations of VKR, one of which was based on parents used in the crossing system and a second based on genotypes from the ancestral breeds that comprise VKR. Variation of breed composition estimates were assessed between pedigree and genome-based predictions. Lastly, Genomic estimations were correlated with production and health traits by comparing extreme performance groups to identify the relationship between breed ancestry and performance. With the exception of the JER breed composition in Grazecross, all other estimates of the purebred contribution to the ProCROSS and Grazecross showed a significant difference in their genomic breed estimation when using the VKR ancestral versus the VKR parental reference populations for admixture analysis. These observations were expected given the different relationship of each VKR representation to the crossbred cattle. Further analysis showed that regardless of which VKR reference population was used, the degree of MON and HOL breed composition plays a significant role in milk and fat production in ProCROSS, while the degree of VKR and NOR ancestry were related to improved health performance in Grazecross. In all, identifying the most appropriate and informative animals to use as reference animals in admixture analysis is an important factor when interpreting results of relationship and population structure, but some degree of uncertainty exists when assessing the relationship of breed composition to phenotypic performance.

Comparison of a three-step in vitro method and the mobile nylon bag technique for determining the intestinal digestibility of rumen-protected products in dairy cows

The objectives of this study were to develop a three-step in vitro method to assess intestinal digestibility of rumen-protected products in dairy cows, and compare this method with the mobile nylon bag technique. A 3 × 4 × 3 factorial experiment was performed to determine an optimal combination of pancreatin (0.8, 1.0, and 1.2 g/30 mL), sodium cholate (0, 1.0, 2.0, and 3.0 g/30 mL), and incubation length (8, 16, 24 h) for maximizing lipid digestion of a hydrogenated fat product. Following the preliminary study, a 23 factorial experiment was conducted to investigate the effects of ruminal incubation (with or without 16 h rumen in situ incubation), gastric pH (pH = 1.0 or 2.0), and gastric acid incubation time (1 or 2 h) on digestion of the hydrogenated fat and a calcium salts of long chain fatty acids product. Once optimal culture parameters were determined, the developed in vitro method was compared with the mobile nylon bag technique. The pancreatin × sodium cholate and sodium cholate × incubation time interactions were significant in the intestinal digestibility of the hydrogenated fat product (P < 0.001), and the best case scenario was observed when the hydrogenated fat was incubated in 30 mL buffer solution containing 1.0 g pancreatin and 2.0 g sodium cholate for 24 h. A ruminal incubation × pH × incubation time interaction was detected for the intestinal digestibility of the hydrogenated fat (P = 0.002), and a ruminal incubation × pH interaction was observed for the calcium salts of fatty acids (P = 0.04). The digestion of hydrogenated fat was maximized when it was incubated in gastric pH 1.0 solution for 2 h following 16 h ruminal incubation. However, ruminal incubation had no effect on the intestinal digestibility of the calcium soap when incubated in gastric pH 1 solution (P < 0.05). The developed in vitro method had greater DM intestinal digestibility than the mobile nylon method (on average 0.78 versus 0.33), implying the mobile nylon bag method might have underestimated the DM intestinal digestibility of rumen-protected products. However, no significant difference was observed on intestinal digestibility of Met, Lys and choline in the rumen fat-protected products. Overall, the developed method could be used as an alternative approach for screening and evaluating intestinal digestibility of rumen-protected products.

PSVI-15 Effects of Different Dietary Sources of Lipids for Lactating Ewes

Abstract The aim of this study was to evaluate the effects of diets with fatty acid profiles on lactation performance and milk composition of ewes. Dorper vs. Santa Inês ewes (n=40; 54.9 ± 8.3 kg BW and 14 days in milk) were penned individually and used in a randomized complete block design. Ewes were fed a basal diet (19.87 ± 0.62% CP) containing 70% concentrate and 30% coastcross hay, and no added oil (CON). The remaining diets were added with 4.0% of soybean oil (SO), calcium salts of palm fatty acids (CSPF), or calcium salts of soybean fatty acids (CSSF) (DM basis). Ewes were fed the diets from the week 2 to 10 of lactation. Milk production was determined every 7 days during the experiment. Ewes were separated from their lambs and mechanically milked for emptying of the mammary gland. After 3 hours, using the same procedure, milk production was recorded and samples collected for milk composition analysis. The addition of SO decreased (P&amp;lt; 0.001) DM intake, with no difference between the other treatments. Milk production, as well as the production of fat, protein, lactose and total solids (g/3 hours) were not affected by the treatments. Regarding milk composition, the percentage of milk fat and total solids increased (P&amp;lt; 0.0001) with CSSF. The percentage of protein was less (P&amp;lt; 0.001) in the milk from ewes that received the diet containing CSPF, with no difference between the other diets. Finally, the percentage of lactose was less (P&amp;lt; 0.001) for ewes fed the diet containing calcium salts of palm fatty acids. In conclusion, soybean oil had the greatest negative effect on dry matter intake. All the oil sources modulated the milk composition, with better results in this regard for the calcium salts of palm fatty acids, considering that it increased the fat and total solids content of the milk.

Overview of the current situation of consumer demand for linseed oil in Kazakhstan

The growth in flaxseed oil consumption is due to the popularity of a healthy lifestyle, the ease of its use in nutrition, and the increased demand of food manufacturers. Flax has gained great popularity as an ingredient for various flour products, jelly, cereals, etc. The purpose of the study is to study the flax oil market in Kazakhstan, the problems and prospects for its development. Methods – industry information from open and closed professional databases, reporting of enterprises, information about their foreign economic activity, publications in scientific literature and the media, consumer and expert surveys, monitoring of wholesale and retail prices, aggregated into a single information array was analyzed. Results – a detailed systematic analysis of market offers of oil of this agricultural crop in the republic in 2017-2021 was carried out, growth rates of its main indicators, trade balance in physical and value terms, production of this type of food in the regions of the Republic of Kazakhstan are presented. External factors affecting consumption and distribution channels are considered. The impact of quarantine restrictions due to the coronovirus pandemic, which disrupted established economic ties along the entire chain from growing oilseeds to their processing, was noted. The main directions for the sale of raw materials and commercial products were identified. Conclusions – the Kazakhstani linen sub-complex in a short period entered the top three world leaders. In the medium term, domestic exporters have the opportunity to strengthen their positions by market structuring and branding their products. It is necessary to carry out indu stry research, including development of the latest formulations of oil and fat products with the involvement of processing facilities; increasing the area under flax cultivation, applying the best farming practices that ensure preservation and increase in the fertility of farmland.

A comparative study of human milk fat substitute from Rhodococcus opacus and plant-oil based commercial products

Human milk fat substitute is an important structured lipid intended for infant formula. This present study compared human milk fat substitute from R. opacus with commercial product and evaluated whether it is qualified for infant formula. It was proven that both human milk fat substitute and commercial product were mainly composed of C16:0 and C18:1 in total fatty acid composition. Human milk fat substitute contained high level of C16:0 at sn-2 position (54.57%) and high level of 1,3-dioleoyl-2-palmitoylglycerol (37.94%), which was in accordance with human milk fat and commercial product. In addition, relative high level of odd chain fatty acids (1.56%) and C16:1 (7.27%) was identified, which made human milk fat substitute unique. Human milk fat substitute was short of medium-chain fatty acids, such as C8:0, C10:0 and polyunsaturated fatty acids, such as, eicosapentaenoic acid and docosahexaenoic acid. In conclusion, human milk fat substitute from R. opacus was qualified as a component for infant formula in term of fatty acid composition and triacylglycerol.

Low Aerobic Capacity Accelerates Lipid Accumulation and Metabolic Abnormalities Caused by High-Fat Diet-Induced Obesity in Postpartum Mice.

Women during pregnancy and postpartum show high rates of obesity and metabolic diseases, especially women with excessive caloric intake. In the past, it was proved that individuals with high intrinsic aerobic exercise capacities showed higher lipid metabolism and lower fat production than those with low intrinsic aerobic exercise capacities. The purpose of this study was to determine whether mice with the low-fitness phenotype (LAEC) were more likely to develop metabolic abnormalities and obesity under dietary induction after delivery, and if mice with a high-fitness phenotype (HAEC) had a protective mechanism. After parturition and weaning, postpartum Institute of Cancer Research (ICR) mice received dietary induction for 12 weeks and were divided into four groups (n = 8 per group): high-exercise capacity postpartum mice with a normal chow diet (HAEC-ND); high-exercise capacity postpartum mice with a high-fat diet (HAEC-HFD); low-exercise capacity postpartum mice with a normal chow diet (LAEC-ND); and low-exercise capacity postpartum mice with a high-fat diet (LAEC-HFD). Obesity caused by a high-fat diet led to decreased exercise performance (p < 0.05). Although there were significant differences in body posture under congenital conditions, the LAEC mice gained more weight and body fat after high-fat-diet intake (p < 0.05). Compared with HAEC-HFD, LAEC-HFD significantly increased blood lipids, such as total cholesterol (TC), triacylglycerol (TG), low-density lipoprotein (LDL) and other parameters (p < 0.05), and the content of TG in the liver, as well as inducing poor glucose tolerance (p < 0.05). In addition, after HFD intake, excessive energy significantly increased glycogen storage (p < 0.05), but the LAEC mice showed significantly lower muscle glycogen storage (p < 0.05). In conclusion, although we observed significant differences in intrinsic exercise capacity, and body posture and metabolic ability were also different, high-fat-diet intake caused weight gain and a risk of metabolic disorders, especially in postpartum low-fitness mice. However, HAEC mice still showed better lipid metabolism and protection mechanisms. Conversely, LAEC mice might accumulate more fat and develop metabolic diseases compared with their normal rodent chow diet (ND) control counterparts.

Identifying new Product Opportunities From Waste: Eliminating Waste in Tomato Production

The challenge of new product development (NPD) has seen a growth in literature on both idea generation and sustainability, but these two areas have not yet fully been integrated. The development of products from agricultural waste has particularly been overlooked, although the need to utilise sustainable materials and reuse waste is increasingly acknowledged. Our paper answers the question: How can different sources of agricultural food waste be utilized to create new product opportunities? We present a new framework for the identification and evaluation of NPD opportunities. This framework offers a new perspective to conceptualise different types of waste and identify their relative sensory, nutritional and functional properties. We present a case study of a UK tomato grower, using data gathered from interviews, attendance of meetings, observations and secondary data. Tomato production creates significant volumes of waste from unripe, imperfect, damaged and overripe fruits. Furthermore, processing tomatoes (for ketchup, passatas, juices, etc.) generates other kinds of waste such as seeds, skins and water. We show how firms can uncover new product opportunities through an analysis of each of these properties. For example, in tomato juice production around 5% of the raw material goes to waste since skins and seeds cannot be used. However, skins and seeds are nutritionally, sensorially and functionally useful and versatile. Nutrition: skins and seeds are rich in essential amino acids, minerals, fatty acids and lycopene, an antioxidant with several reported health benefits. Sensory: skin contains crystalline cellulose which can improve mouthfeel of food in low fat products, while seeds can increase hardness in flat breads. Functional: skins are partially permeable to liquids and gases, giving them potential for packaging and biodegradable tableware, while seed powder can be effective in removing organic dye molecules from coloured textile effluents. On the basis of our analysis we conclude that by examining the nutritional, sensory and functional characteristics of tomato waste, this can lead NPD managers to explore new alternatives in industries different from the original source of waste.

PORK QUALITY AND GENETIC ASSOCIATION STUDY OF PORCINE LEPTIN AND CATHEPSIN F GENE POLYMORPHISMS

This study aims to assess the quality of raw pork intended for further processing, and also to analyse associations of genotypes, determined by single-nucleotide polymorphisms (SNPs) in leptin (LEP g.2845 A &gt; T) and cathepsin (CTSF g.22 C ≤ G ) genes, with meat and fat quality traits.&#x0D; Meat and fat products as raw materials for further processing, produced from pigs of French origin bred on the pig farm “Artsyz Meat Company Ltd” located in Artsyz district of Odesa region, are considered as the object of this study. The total number of pigs used to perform DNA analysis is 350 heads. DNA tests and physicochemical analyses of meat and fat products were conducted at the research laboratory of the Institute of Pig Breeding and Agricultural Production of the National Academy of Agrarian Sciences of Ukraine in Poltava. Genomic DNA was extracted from pig bristle using the Chelex 100 ion exchange resin. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used for genotyping. Fattening pigs were assessed for leptin (LEP g.2845) and cathepsin F (CTSF g.22) genes. Upon the young stock’s reaching the target live weight of 100 kg, 60 pigs in each group genotyped for the target genes were slaughtered using conventional methods. The results of genetic tests and association studies performed have shown that leptin and cathepsin F gene polymorphisms produce an effect on particular pork and backfat quality attributes.&#x0D; Statistically significant associations of the genetic marker LEP SNP g.2845 A &gt; T with the investigated quality attributes of meat and fat products, in particular water-holding capacity, meat tenderness, intramuscular fat content, backfat moisture content and melting point, have been detected. Meanwhile, the genetic marker CTSF SNP g.22 C ≤ G is found to be associated with such pork quality attributes as water-holding capacity, tenderness, weight loss during thermal processing (cooking loss), intramuscular fat content, calcium and phosphorus levels, and energy value.

Influence of taste components on the safety of butter

Fatty foods can be considered healthy food formulas if they meet the following conditions: have a reduced calorie content; balanced fatty acid composition; vitamins and biologically active compounds; They do not contain cholesterol or trans fats. To give a functional orientation to butter, additional flavoring components can be introduced. The authors conducted research to study the storage capacity of butter with flavoring components. As fillers, a mixture of seeds and herbs was added to it. The resulting oil samples had a pronounced creamy taste with a taste of the added filler, without foreign tastes and odors, a dense, plastic texture, a shiny surface on the cut, dry in appearance, with the inclusion of filler particles. The mass fraction of product fat was 62%. The filler has antioxidant activity, reduces the spoilage of the fat fraction during storage. Determination of the resistance of fat at 102 ºС was carried out to test its ability to oxidative processes. The test sample was stored without signs of spoilage for 10 hours, the control one for 8 hours. Studies of the oil acidity index during storage have been carried out. It was found that at the end of the shelf life, the acidity index of the oil was 0.5 ± 0.1 °K less than that of the control sample. Tested for mold susceptibility. The absence of mold after 14 days of storage indicated the relative resistance of the oil to mold. Thus, the use of non-traditional plant components in the production of oil and fat products contributes to the expansion of their range and quality.

Impact of Feeding Probiotics on Blood Parameters, Tail Fat Metabolites, and Volatile Flavor Components of Sunit Sheep.

Sheep crude tail fat has unique nutritional values and is used as a raw material for high-quality natural oil. The purpose of this study was to investigate the effects of probiotics on the metabolites and flavor of sheep crude tail fat. In this study, 12 Sunit sheep were randomly divided into an experimental group (LTF, basal feed + Lactiplantibacillus plantarum powder) and a control group (CTF, basal feed). The results of sheep crude tail fat analysis showed that blood lipid parameters were significantly lower and the expression of fatty acid synthase and stearoyl-CoA desaturase genes higher in the LTF group than in the CTF group (p < 0.05). Metabolomic analysis via liquid chromatography–mass spectrometry showed that the contents of metabolites such as eicosapentaenoic acid, 16-hydroxypalmitic acid, and L-citrulline were higher in the LTF group (p < 0.01). Gas chromatography–mass spectrometry detection of volatile flavor compounds in the tail fat showed that nonanal, decanal, and 1-hexanol were more abundant in the LTF group (p < 0.05). Therefore, Lactiplantibacillus plantarum feeding affected blood lipid parameters, expression of lipid metabolism-related genes, tail fat metabolites, and volatile flavor compounds in Sunit sheep. In this study, probiotics feeding was demonstrated to support high-value sheep crude tail fat production.

Utilization of whey in bakery products-A review

Whey is a vast repository of valuable nutrients and serves as a key ingredient in the bakery sector. Whey being a significant by product of the dairy industry is necessary to be utilized to reduce the burden of its disposal on the environment, which is attributed to the high BOD of whey. Whey based ingredients find an ocean of application in foods. Whey based ingredients have superior functional properties attributable to whey proteins which results in enhancement of textural and chemical properties of products they are applied in. Whey proteins are used as fat replacers, emulsifiers, flavour and appearance enhancers etc in a wide category of bakery products. Whey based ingredients can negate the use of dough conditioners due to high water binding capacity which aids in developing improved texture and fine even crumb. Whey ingredients can boost levels of protein, calcium, potassium and other minerals in bakery products. With increasing demand for clean label products and low fat products with the same textural attributes as full fat ones the demand for whey ingredients is surging. In this article we review the applications of whey based ingredients in bakery products.