Fat Line Research Articles

Low protein diets for broilers

A review of the literature regarding Low Protein diets (LCP) for broilers reveals that by using crystalline amino acids, dietary CP could be reduced in each phase safely by a factor of 10% (a figure fairly consistent with turkeys but quite low with that found for ducks and swine) from the respective NRC (1994) levels; the calculated minimum dietary CP levels thus appeared to be 20.7, 18.0, and 16.2% respectively for 0–21, 21–42 and 42–56 days of age. It is, however, noted that the magnitude of protein reduction, without affecting the growth performance of broilers, could have been increased depending upon the EAA concentration and balance of LCP vs. control-CP diets as well as the response criteria used (gain vs. composition of gain). Further reduction in dietary protein results in depressed live/carcass yield in almost all cases. Depressed feed intake appears partially to explain the negative effect of LCP diets in some cases. Addition of NEAA-like glycine above the current NRC recommendations has shown partially to alleviate the negative effect of LCP diets indicating imprecision of our knowledge about NEAA (like glycine, serine and proline etc) requirements of broilers under these conditions. The dietary NEAA/EAA ratio seems to be an important, though poorly understood, factor in defining the performance of broilers fed on LCP diets. It seems that regardless of the absolute amounts of dietary amino acids, performance tend to be depressed when NEAA/EAA ratio is decreased from 50/50; thus the NEAA level of about 50% of protein seems “minimum” state of adequacy. More work is needed before a precise role of the ratio of nitrogen coming from NEAA vs. EAA is proposed. A wider NE:ME in LCP diets seems to explain the higher body fat accretion on these diets; more work on how dietary NE:ME ratio modulates the growth performance of broilers, and how it interacts with different genetic stocks (lean vs. fat lines) is warranted.

A partial genome scan to map quantitative trait loci for carcass composition, as assessed by X-ray computer tomography, and meat quality traits in Scottish Blackface Sheep

AbstractQuantitative trait loci (QTL) were identified for traits related to carcass and meat quality in Scottish Blackface sheep. The population studied was a double backcross between lines of sheep divergently selected for carcass lean content (LEAN and FAT lines), comprising nine half-sib families. Carcass composition (600 lambs) was assessed non-destructively using computerized tomography (CT) scanning and meat quality measurements (initial and final pH of m. semimembranosus, colour, shear force value, carcass weight, lamb flavour, juiciness, tenderness and overall liking) were taken on 300 male lambs. Lambs and their sires were genotyped across candidate regions on chromosomes 1, 2, 3, 5, 14, 18, 20 and 21. QTL analyses were performed using regression interval mapping techniques. In total, nine genome-wise significant and 11 chromosome-wise and suggestive QTL were detected in seven out of eight chromosomes. Genome-wise significant QTL were mapped for lamb flavour (OAR 1); for muscle densities (OAR 2 and OAR 3); for colour a*(redness) (OAR 3); for bone density (OAR 1); for slaughter live weight (OAR 1 and OAR 2) and for the weights of cold and hot carcass (OAR 5). The QTL with the strongest statistical evidence affected the lamb flavour of meat and was on OAR 1, in a region homologous with a porcine SSC 13 QTL identified for pork flavour. This QTL segregated in four of the nine families. This study provides new information on QTL affecting meat quality and carcass composition traits in sheep, which may lead to novel opportunities for genetically improving these traits.

Fatness QTL on chicken chromosome 5 and interaction with sex

Quantitative trait loci (QTL) affecting fatness in male chickens were previously identified on chromosome 5 (GGA5) in a three-generation design derived from two experimental chicken lines divergently selected for abdominal fat weight. A new design, established from the same pure lines, produced 407 F2 progenies (males and females) from 4 F1-sire families. Body weight and abdominal fat were measured on the F2 at 9 wk of age. In each sire family, selective genotyping was carried out for 48 extreme individuals for abdominal fat using seven microsatellite markers from GGA5. QTL analyses confirmed the presence of QTL for fatness on GGA5 and identified a QTL by sex interaction. By crossing one F1 sire heterozygous at the QTL with lean line dams, three recombinant backcross 1 (BC1) males were produced and their QTL genotypes were assessed in backcross 2 (BC2) progenies. These results confirmed the QTL by sex interaction identified in the F2 generation and they allow mapping of the female QTL to less than 8 Mb at the distal part of the GGA5. They also indicate that fat QTL alleles were segregating in both fat and lean lines.

Genetic analyses of carcass composition, as assessed by X-ray computer tomography, and meat quality traits in Scottish Blackface sheep

AbstractGenetic parameters for carcass composition and meat quality traits were estimated in Scottish Blackface sheep, previously divergently selected for carcass lean content (LEAN and FAT lines). Computerized X-ray tomography (CT) was used to obtain non-destructivein vivoestimates of the carcass composition of 700 lambs, at ca. 24 weeks of age, with tissue areas and image densities obtained for fat, muscle and bone components of the carcass. Comprehensive measures of meat quality and carcass fatness were made on 350 male lambs, at ca. 8 months of age, which had previously been CT scanned. Meat quality traits included intramuscular fat content, initial and final pH of the meat, colour attributes, shear force, dry matter, moisture and nitrogen proportions, and taste panel assessments of the cooked meat. FAT line animals were significantly (P<0·05) fatter than the LEAN line animals in all measures of fatness (from CT and slaughter data), although the differences were modest and generally proportionately less than 0·1. Correspondingly, the LEAN line animals were superior to the FAT line animals in muscling measurements. Compared with the LEAN line, the FAT line had lower muscle density (as indicated by the relative darkness of the scan image), greater estimated subcutaneous fat (predicted from fat classification score) at slaughter, more intramuscular fat content, a more ‘yellow’ as opposed to ‘red’ muscle colour, and juicer meat (allP<0·05). All CT tissue areas were moderately to highly heritable, with h2values ranging from 0·23 to 0·76. Likewise, meat quality traits were also moderately heritable. Muscle density was the CT trait most consistently related to meat quality traits, and genetic correlations of muscle density with live weight, fat class, subcutaneous fat score, dry matter proportion, juiciness, flavour and overall liking were all moderately to strongly negative, and significantly different from zero. In addition, intramuscular fat content was positively genetically correlated with juiciness and flavour, and negatively genetically correlated with shear force value. The results of this study demonstrate that altering carcass fatness will simultaneously change muscle density (indicative of changes in intramuscular fatness), and aspects of intramuscular fat content, muscle colour and juiciness. The heritabilities for the meat quality traits indicate ample opportunities for altering most meat quality traits. Moreover, it appears that colour, intramuscular fat content, juiciness, overall liking and flavour may be adequately predicted, both genetically and phenotypically, from measures of muscle density. Thus, genetic improvement of carcass composition and meat quality is feasible using in vivo measurements.

Microarray analysis of differential gene expression in the liver of lean and fat chickens

Excessive adiposity has become a major drawback in meat-type chicken production. However, few studies were conducted to analyze the liver expression of genes involved in pathways and mechanisms leading to adiposity. A previous study performed by differential display on RNAs extracted from chicken livers from lean and fat lines allowed us to isolate cDNA products of genes with putative differential expression. In this study, a cDNA microarray resource was developed from these products together with cDNAs from genes involved in or related to lipid metabolism. This resource was used to analyze gene expression in the liver from lean and fat chickens. Some genes were found with a difference in expression between lean and fat animals and/or correlated to adipose tissue weight. Cytochrome P450 2C45, thought to play a role in biotransformation of steroids and poly-unsaturated fatty acids, was more expressed in lean chickens whereas fatty acid synthase, stearoyl-CoA desaturase, sterol response element binding factor 1 and hepatocyte nuclear factor 4, respectively involved in lipogenesis and its regulation, were more expressed in fat chickens. These results indicate that mechanisms involved in the expression and regulation of lipogenic genes could play a key role in fatness ontogenesis in chickens from lean and fat lines.

Growth hormone and ghrelin receptor genes are differentially expressed between genetically lean and fat selection lines of sheep

The objective of this study was to determine whether differences in mRNA levels of key pituitary genes that regulate GH production, pituitary development, and growth were present and/or associated with divergent body composition phenotypes observed between sheep from genetically divergent lean and fat selection lines. Real-time PCR transcription profiles for pituitary specific transcription factor 1, prophet of pit1, GH, GH receptor, GH secretagogue receptor, GHRH receptor, leptin receptor, and somatostatin receptors 1 and 2 were determined in pituitary tissue. There was a difference in the amount of both GH (P < 0.001) and GH secretagogue receptor (P < 0.001) mRNA between the selection lines (5 females and 5 males per line; 20 wk of age); the lean line had greater abundance than the fat line, irrespective of which endogenous control gene was used. The results obtained for GHRH receptor were equivocal but suggestive; there were greater GHRH receptor mRNA levels (P < 0.001) in the lean line using beta-2-microglobulin as the endogenous control but not when hypoxanthine phosphoribosyltransferase and glyceraldehyde-3-phosphate dehydrogenase were used. No difference in pituitary specific transcription factor 1, prophet of pit1, GH receptor, leptin receptor, or somatostatin receptors 1 and 2 mRNA concentration was observed between the lines. The greater abundance of GH mRNA in the pituitary somatotropes from genetically lean animals appears to be associated with increased levels of GH secretagogue receptor mRNA and possibly GHRH receptor mRNA. This suggests that the difference in GH secretion between the lines may be due to differences in the afferent signals, such as ghrelin and/or GHRH, arising from the hypothalamus, or as a result of differential pituitary sensitivity to these hormones.

Comparative study on the radiographic and CT findings of peroxyacetic acid corrosive esophagitis

目的 探讨过氧乙酸腐蚀性食管炎的X线和CT特征.方法分析7例过氧乙酸烧伤的消化道X线和CT资料.结果 7例过氧乙酸腐蚀性食管炎主要X线表现为食管中下段管腔严重不规则狭窄,管壁僵硬、蠕动消失,正常黏膜皱襞消失,狭窄以上食管呈不同程度扩张;主要CT表现为中下段狭窄区管壁呈不规则性明显增厚,密度偏低,外缘模糊,周围脂肪线消失,食管上段有不同程度扩张.结论 X线和CT检查可以清楚显示狭窄段部位、范围和程度,食管壁及周围受累情况,以指导临床制定治疗方案。

Regulators Acting in Combinatorial Codes Also Act Independently in Single Differentiating Neurons

In the Drosophila ventral nerve cord, a small number of neurons express the LIM-homeodomain gene apterous (ap). These ap neurons can be subdivided based upon axon pathfinding and their expression of neuropeptidergic markers. ap, the zinc finger gene squeeze, the bHLH gene dimmed, and the BMP pathway are all required for proper specification of these cells. Here, using several ap neuron terminal differentiation markers, we have resolved how each of these factors contributes to ap neuron diversity. We find that these factors interact genetically and biochemically in subtype-specific combinatorial codes to determine certain defining aspects of ap neuron subtype identity. However, we also find that ap, dimmed, and squeeze additionally act independently of one another to specify certain other defining aspects of ap neuron subtype identity. Therefore, within single neurons, we show that single regulators acting in numerous molecular contexts differentially specify multiple subtype-specific traits.

Hypothalamo‐pituitary‐adrenocortical axis in stress‐susceptible and stress‐resistant pigs: endocrine responses to corticotrophin‐releasing factor and vasopressin*

Swiss Landrace pigs selected into genetically well-characterized low and high tissue fat lines (f and F respectively) react differently to exogenous and endogenous stressors. Response of the hypothalamo-pituitary-adrenocortical (HPA) axis to i.v. administered ovine corticotrophin-releasing factor (oCRF) and lysine vasopressin (LVP) in young females, intact and pretreated with dexamethasone or metyrapone, leads to the conclusion that different stress susceptibility of the two lines correlates with the sensitivity of pituitary corticotrophs to oCRF stimulation. Total amount of ACTH released after stimulation with submaximal oCRF doses was roughly equal in both lines, and cortisol level is even lower in the f-line, most likely due to the considerably enhanced metabolic clearance rate of cortisol (lower half-life of plasma cortisol compared with F-line). LVP-stimulated ACTH release is comparable with that of oCRF is stronger in the f-line. Combined effect of oCRF and LVP is rather additive than synergistic but the half-life ratio cortisol/ACTH after this stimulation is about four times higher than for stimulation by LVP and oCRF separately. In cases of externally stimulated HPA axis, cortisol plasma concentration tightly cross-correlates with that of ACTH.

Two-way selection for muscle lipid content in pan-size rainbow trout ( Oncorhynchus mykiss)

A two-way selection for muscle lipid content was performed in rainbow trout. One-year-old pan-size individuals were mass selected using Distell Fish Fatmeter values corrected for absolute weight (fat index, FI), this being a non-destructive measurement of the muscle lipid content in live fish. Direct and correlated responses at pan-size (around 260 g) were estimated after two generations of selection. Absolute Fat values and FI values in the upward line (fat line, FL) and downward line (lean line, LL) differed from the control line and from each other. The mean realized heritability achieved for FI was 0.25. The difference in Fat values between FL and LL resulted in a significant difference in muscle lipid content (29.6% dry matter content in FL, vs. 25.6% in LL, P=0.003). The differences in Fat and FI values between FL and LL were sustained until maturation at 2 years old. At the age of 1 year, the two lines did not differ significantly in terms of weight, length or most body shape traits (height, width or cross-section shape). However, FL fish had a better condition factor than LL (1.44 vs. 1.38), and a greater belly thickness (11% relative increase), the control line displaying intermediate values. The abdominal internal fat coat was thicker in FL, and represented a larger proportion of the entire abdominal thickness than in LL. The relative visceral weight (in % of total body weight), carcass and fillet yield were not modified by selection. The frequency of precocious males was significantly higher in FL (16.5%, vs. 11% and 10.1% in control and LL fish, respectively), but there was no difference between the reproductive traits of 2-year-old LL and FL females. These results indicate that breeding based on Fatmeter values was efficient in selecting the fillet lipid content in rainbow trout, with limited correlated responses at the size where selection was applied. In particular, no adverse effects were recorded of a reduction in muscle lipid content on carcass or fillet yield under the experimental conditions applied during this study.

The mouse as a model for understanding the regulation of body composition

Body composition of animals and man is generally assumed to be a regulated phenomenon. Understanding the factors that are involved in such regulation is important in at least two different contexts. The obesity epidemic sweeping across developed nations has been described by the WHO as the greatest health threat facing Western societies. Understanding the underlying physiological factors that lead sectors of the population to fail in their attempts to regulate body mass and composition are of key importance in the drive to develop pharmaceutical remedies for this serious condition. A knock on effect for the agricultural sector however is the consumer demand for leaner animal products. This places a premium on understanding how body composition is regulated in livestock. Moreover the financial rewards for improving the energetic efficiency of production provide an additional incentive for understanding the details of energy regulation that underlie control of body composition. While direct genetic and physiological studies of livestock are feasible the mouse provides a convenient model animal that can inform our understanding of the conserved physiological mechanisms in both man and other animals. The key advantages of using the mouse in this context are that its small size and short breeding cycle enable experiments to be performed rapidly. Measuring physiological components of energy balance in mice is easily performed. In addition the mouse genome has been sequenced and the tools for performing large scale gene expression studies are already commercially available, opening up the capacity to perform integrative physiological studies form the level of the genome to the whole animal. Several genetic models of mice are known which have monogenic forms of extreme obesity. The best known of these is the ob/ob mouse, which is deficient in production of the adipokine leptin. However, many other single gene mutants are known which generate similar effects. Dissecting the loci of such effects has enabled us to construct a working model of how adiposity is signalled in the brain via the melanocortin system and how feedback loops including this system may contribute to regulation of energy balance. While some individuals have been identified that have similar genetic disruptions these are very rare, and it is clear that variation in human and livestock body composition generally reflects polygenic effects. More useful models may therefore be mice that have been selected for many generations for traits that impact on their body composition. The short generation time of the mouse has been extremely useful in the generation of such lines. My own group has been collaborating with the University of Edinburgh where such long-term selection experiment was initiated in the 1970s. We have in particular been quantifying the energy balance of mice long term selected for fat and lean body composition. We first showed that these polygenic effects do not seem to include polymorphisms within the leptin signalling system. By comparing total energy intake, with measures of resting energy expenditure the contribution of different energy compartments to energy balance can be assessed. These studies indicated that the major difference in energy regulation between the lines is that the lean line diverts substantially more energy into activity than the fat line. Direct measures of activity have been made to confirm this effect with mixed results. Using passive infrared detection devices the converse result is found – that the lean mice are less active, but using running wheels the logged activity matches the measured energy balance. These data indicate that the factors influencing energy balance reflect complex polygenic effects that reach far outside the leptin signalling system. The mouse is an ideal tool to explore these effects with implications for both the livestock industry and the obesity epidemic.

Microarray gene expression analysis of theFob3bobesity QTL identifies positional candidate geneSqleand perturbed cholesterol and glycolysis pathways

Obesity-related diseases are poised to become the primary cause of death in developed nations. While a number of monogenic causes of obesity have recently been identified, these are responsible for only a small proportion of human cases of obesity. Quantitative trait locus (QTL) studies using animal models have revealed hundreds of potential loci that affect obesity; however, few have been further analyzed beyond the original QTL scan. We previously mapped four QTL in an F(2) between divergently selected Fat (F) and Lean (L) lines. A QTL of large effect on chromosome 15 (Fob3) was subsequently mapped to a higher resolution into two smaller-effect QTL (Fob3a and Fob3b) using crosses between the F-line and a congenic line containing L-line alleles at the Fob3 QTL region. Here we report the gene expression characterization of Fob3b. Microarray expression analysis using the NIA-NIH 15K cDNA array set containing 14,938 mouse ESTs was employed to identify candidate genes and pathways that are differentially expressed between the F-line and a congenic line containing only the Fob3b QTL (Fob3b-line). Our study suggests squalene epoxidase (Sqle), a cholesterol biosynthesis enzyme, as a strong positional candidate gene for Fob3b. Several other cholesterol biosynthesis pathway genes unlinked to Fob3b were found to be differentially expressed, suggesting that a perturbation of this pathway could be in part responsible for the phenotypic difference between the F-line and Fob3b-line mice.

Genetic complexity of an obesity QTL ( Fob3) revealed by detailed genetic mapping.

Obesity is proving to be a serious health concern in the developed world as well as an unwanted component of growth in livestock production. While recent advances in genetics have identified a number of monogenic causes of obesity, these are responsible for only a small proportion of human cases of obesity. By divergent selection for high and low fat content over 60 generations, we have created Fat (F) and Lean (L) lines of mice that represent a model of polygenic obesity similar to the situation in human populations. From previous crosses of these lines, four body fat quantitative trait loci (QTL) were identified. We have created congenic lines (F(chr15L)), by recurrent marker-assisted backcrossing, to introgress the QTL region with the highest LOD score, Fob3 on Chr 15, from the L-Iine into the F-line background. We have further mapped this QTL by progeny testing of recombinants, produced from crosses between the F-line and congenic F(chrl5L) mice, showing that the Fob3 QTL region is a composite of at least two smaller effect QTL-the proximal QTL Fob3a is a late-onset obesity QTL, whereas the distal Fob3b is an early-onset obesity QTL.

Genetic linkage and expression analysis of SREBP and lipogenic genes in fat and lean chicken

To identify the genes directly responsible, through DNA polymorphism, for the difference in fatness observed between a lean and a fat chicken line, we studied five genes ( ACL, ACC, FAS, ME, SCD1) encoding key enzymes involved in liver fatty acid synthesis and secretion. Genetic linkage was tested between polymorphic sites in the genes and the fatness trait segregating in an F2 design obtained by inter-crossing the two fat and lean lines. Despite a confirmation of a higher mRNA level in the fat birds, no genetic linkage of the gene alleles with the phenotype could be found. As a test of the implication of upstream regulatory transcription factors, SREPB genes were also studied. The lack of genetic linkage of SREBP genes with fatness shows that these genes are not directly responsible through polymorphism for fatness variability in our model. Moreover, the similar SREBP mRNA levels observed between the two lines led us to exclude also transcriptional factors regulating the two SREBP genes as being directly responsible for fatness variability. However, the genes involved in post-translational modifications of SREBPs remain candidates to investigate. These results emphasised the interest to perform expression and genetic linkage studies jointly, to progress in identifying the genetic origin of variability of a quantitative trait.

Belief vs. scientific observation: the curious story of the precardiac mesoderm.

Belief vs. scientific observation: the curious story of the precardiac mesoderm.

Genetic Differences in Energy Partitioning in Growing Pigs

Energy partitioning was studied in pigs differing in potential for carcass lean growth and fatty tissue content from 25 to 105 kg body weight, by the means of repeated measures of detailed body composition and individual feed intake. In total, 141 pigs were included from the three genetic groups Norwegian Landrace (lean and efficient), Duroc and Landrace 2 LP (a fat and slow-growing selection line) (LLP). Individual feed consumption was registered, and detailed body composition measured repeatedly by computed tomography. Energy consumption [MJ metabolizable energy (ME) day -1 ] did not differ between the genetic groups. In general, about 40-50% of consumed energy was partitioned to growth. The genetic groups partitioned equal proportions of daily energy consumption to growth (ME GROWTH ) and maintenance (MEm) early and late in the growth period. From 50 to 85 kg body weight Landrace partitioned more to growth and less to maintenance compared with Duroc ( P <0.05). When considering partitioning of ME above maintenance, the genetically fat LLP had the highest net energy retention relative to the heat increment of feeding and was therefore the most efficient, Duroc was in an intermediate position while the lean Landrace had the lowest proportion. The partitioning of ME GROWTH to fatty tissue and carcass lean growth differed between the genetic groups ( P <0.001) according to genetic potential for carcass lean and fatty tissue gain. Increasing proportions were partitioned to fatty tissue growth with increasing body weight. The genetic groups partitioned equal proportions of ME GROWTH to non- fat visceral components (NFVC) growth. MEm varied between 0.65 and 0.68 MJ kg -0.75 day -1 . MEm increased with increasing weight of carcass lean and viscera ( P <0.01), more so in the modern breeds than in the LLP.

Stearoyl-CoA Desaturase 1 Coding Sequences and Antisense RNA Affect Lipid Secretion in Transfected Chicken LMH Hepatoma Cells

Hepatic stearoyl CoA desaturase (SCD) activity in chickens from a fat line is higher than that of chickens from a lean line and correlates with plasma triacylglycerol concentrations. Furthermore, in these lines, the hepatic SCD1 mRNA level is positively correlated with the adipose tissue weight. To analyze the contribution of the SCD1 gene in the regulation of adiposity in the early stages of triacylglycerol secretion, SCD1 coding sequence and antisense RNA expression vectors were transfected in LMH cells. After selection, these cells were analyzed with regard to SCD1 expression and lipid secretion. The amounts of secreted triacylglycerols and phospholipids were shown to be higher in LMH cells transfected with the SCD1 gene, but reduced in those transfected with the SCD1 antisense sequences when compared to cells transfected with the vector alone (without SCD1 sequences). These results provide direct evidence that the expression of the SCD1 gene plays a major role in the triacylglycerol and phospholipid secretion process.

The effect of restricted feeding on growth hormone (GH) secretory patterns in genetically lean and fat wether lambs

AbstractThe aim of this work was to determine whether developmental changes in growth hormone (GH) secretory patterns and carcass composition were influenced by nutrition and genotype in sheep. Four-month-old wether lambs from lean (low backfat), fat (high backfat) and control selection lines were nutritionally restricted to maintain a 28 kg live weight or given food ad libitum for 24 weeks. Plasma concentrations of GH and insulin-like growth factor 1 (IGF-1) were measured at predetermined times and carcass composition of the animals determined at the end of the trial.From week 3 on, restrictions in dry matter (DM) intake were observed as the ad libitum treatment group had a significantly greater intake than the restricted treatment group (7·70 v. 5·80 kg DM per week, s.e.d. = 0·81). Differences in live weight between the feeding treatments were significant (P &lt; 0·05) at week 9. The restricted feeding regime was associated with significant reductions in plasma levels of IGF-1 but had no effect (P &gt; 0·05) on carcass weight-adjusted carcass fat proportion at the close of the trial. The effect of food restriction on GH secretory patterns was variable. Although there was initially a suppression in mean plasma GH, there was subsequently significantly higher mean plasma GH in the restricted feeding treatment. Periodogram analysis indicated that both the absolute levels of GH and the GH secretory pattern were altered by restricted feeding. In all animals, mean and basal GH concentrations, as well as the frequency and amplitude of pulses, declined from February to March and then increased from May to July (P &lt; 0·001).DM intake and live weight did not differ (P &gt; 0·05) between genotypes, however the fat genotype had greater carcass fatness than lean or control genotypes (P &lt; 0·01). There were no consistent differences between genotypes in plasma IGF-1 concentrations. In the ad libitum treatment, the lean and control genotypes had higher plasma GH levels than the f at genotype but the pattern of GH release did not vary. Under restricted feeding, both the pattern and the level of plasma GH varied between genotypes.It is concluded that the developmental change in GH secretory patterns is affected by nutrition but not in a consistent manner. Although restricted feeding resulted in higher mean plasma GH concentrations later in the trial, this did not result in a change in carcass composition. The biological cues which lead to increased fat deposition in older lambs need further study but plasma GH levels may not he an important mechanism in this process.

Messenger RNA levels and transcription rates of hepatic lipogenesis genes in genetically lean and fat chickens.

Levels of body fat content in commercial meat chickens have prompted research in order to control the development of this trait. Based on experimentally selected divergent lean and fat lines, many studies have shown that liver metabolism has a major role in the fatness variability. In order to identify which genes are involved in this variability, we investigated the expression of several genes implicated in the hepatic lipid metabolism. The studied genes code for enzymes of fatty acid synthesis [ATP citrate-lyase (ACL), acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), malic enzyme (ME), stearoyl-CoA desaturase (SCD1)], for an apolipoprotein [apolipoprotein A1 (APOA1)], and for the CCAAT/enhancer binding protein α (C/EBPα), which is a transcription factor implied in the regulation of several genes of lipid metabolism. The results show that the fat-line chickens display significantly higher hepatic transcription rates and mRNA levels than the lean-line chickens for the ACL, ME and APOA1 genes. This suggests that these genes could be responsible for the phenotypic fatness variability.

NEW METHOD FOR DETERMINING INTERNAL TEMPERATURE OF COOKING MEAT VIA NMR SPECTROSCOPY

ABSTRACTNMR spectroscopy is used to determine fat content and internal temperature of hamburger, pork sausage, and various cuts of chicken during cooking. The temperature is determined by the frequency difference of the water and fat lines within the NMR spectrum. This method is more accurate than the absolute measurement of either peak alone. Furthermore, because the measurement relies on a frequency difference, precise control of the magnetic field is unnecessary. The hamburger and sausage data indicate an accuracy of ± 1C. The temperature accuracy for chicken is ± 5C. The difference in accuracy is due to the lower fat content of chicken. Chicken bone marrow, however, has a high fat content which could be used to measure its temperature. The speed and accuracy of this technique admits the possibility of using NMR spectroscopy on the process line to determine when these cuts of meat are sufficiently cooked and thus safe.