Fastidious Strains Research Articles

Avian egg-shell membrane as a culture medium for dermatophytes.

The purpose of this paper is to describe a new procedure for growing dermatophytes, to point out a number of advantages of the method, and to suggest applications to which it may be adapted. Although most of the dermatophytes may be satisfactorily grown on Sabouraud's glucose agar medium or on modifications of it, direct inoculation from clinical material frequently results in a heavy growth of contaminants, principally bacteria. For this reason it is common practice to make the initial inoculation on media containing bacteriostatically active additives, such as Littman oxgall agar, and media containing antibiotics. Although these media repress the growth of the contaminants, there is reason to believe that the active materials may not always be without effect on the more fastidious strains of the dermatophytes, which could be hindered in their growth and possibly overlooked. It seems logical to believe that

Inhibition of a Strain of Brucella abortus by Medium Filtered through Cotton

The study of cotton as a possible source of factors inhibitory for the growth of Brucella abortus was suggested by observations made at the Department of Agriculture's Animal Disease Station at Beltsville, Md. It was noted there that a potato-infusion agar medium clarified by centrifugation gave excellent growth of Br. abortus, whereas the same medium filtered through eotton was inferior in supporting growth of the organism. In order to determine the nature of the inhibition resulting from filtration through cotton, the effect of this procedure on the grow-th of a fastidious strain of Br. abortus on a simple and reproducible medium was studied. Materials and Methods