Eastern Farmers Research Articles

Interpreting the demic diffusion of early farming in Europe with a three-population model

In 1971, Ammerman and Cavalli-Sforza demonstrated that reaction-diffusion equations could be usefully applied to the archaeological question of the spread of early farming in Europe. Their basic premise was demic diffusion, i.e., the iterative short-range colonization of virgin land by the descendants of the original Near Eastern farmers. This hypothesis has been vindicated by ancient DNA studies, which show limited acculturation of the autochthonous hunter-gatherers, who when converted to farming were apparently assimilated into preexisting farming communities. In this brief report, I describe a reaction-diffusion model incorporating various interactions among the Near Eastern farmers, converted farmers, and hunter-gatherers. Predictions, derived in terms of the model parameters, are examined vis-à-vis the ancient DNA and archaeological evidence. Of particular interest is the theoretical requirement that the hunter-gatherers behaved more competitively toward the converted farmers than the Near Eastern (specifically Anatolian) famers. Based on “Ammerman AJ, Cavalli-Sforza LL. Measuring the rate of spread of early farming in Europe. Man 1971; 6: 674-688.”

Influence of altitude as a proxy for temperature on key Musa pests and diseases in watershed areas of Burundi and Rwanda

Pests and diseases are key biotic constraints limiting banana production among smallholder farmers in Eastern and Central Africa. Climate changemay favour pest and disease development and further exacerbate the vulnerability of smallholder farming systems to biotic constraints. Information on effects of climate change on pests and pathogens of banana is required by policy makers and researchers in designing control strategies and adaptation plans. Since altitude is inversely related to temperature, this study used the occurrence of key banana pests and diseases along an altitude gradient as a proxy for the potential impact of changes in temperature associated with global warming on pests and diseases. We assessed the occurrence of banana pests and diseases in 93 banana fields across three altitude ranges in Burundi and 99 fields distributed in two altitude ranges in Rwanda watersheds. Incidence and prevalence of Banana Bunchy Top Disease (BBTD) and Fusarium wilt (FW) was significantly associated with temperature and altitude in Burundi, revealing that increasing temperatures may lead to upward movement of banana diseases. No significant associations with temperature and altitude were observed for weevils, nematodes and Xanthomonas wilt of banana (BXW). Data collected in this study provides a baseline to verify and guide modelling work to predict future pest and disease distribution according to climate change scenarios. Such information is useful in informing policy makers and designing appropriate management strategies.

Determinants of access to agricultural credit among smallholder rice and maize farmers in the eastern and western provinces of Rwanda

Agricultural credit is believed to play a catalytic role in enhancing agricultural productivity; however, its access is limited for smallholder farmers in Rwanda. To investigate this phenomenon, this study sought to identify and assess the determinants of access to agricultural credit among rice and maize smallholder farmers in Rwanda. The study was conducted in the eastern and western provinces of Rwanda using a cross-sectional survey design. Sample districts, sectors, and cells were obtained using stratified random sampling techniques. Convenient and purposive samplings were used to sample households and farmers, respectively. Data were collected using structured interviews and questionnaires, and were analyzed using a binary logistic regression model. Model results indicated that both individual and institutional factors determine access to agricultural credit among smallholder maize and rice farmers in eastern and western provinces of Rwanda. The individualfactors included: saving of money in commercial banks (Adjusted Odds Ratio (AOR) = 2.389), owning a size of land that is 0-0.1 ha (AOR = 0.127), and knowledge of the repayment terms of agricultural loans (AOR = 0.203), while the institutional factors included: having privately-owned finance institutions in the area (AOR = 0.287), offer of both long and short-term loans (AOR = 0.290), interest rate between 11-15% (AOR = 0.178), the process for obtaining agricultural credit not being too long (AOR = 2.026). Institutional factors were more important than the individual farmer characteristics in determining access to credit. Policy interventions aimed at bolstering agricultural credit access among the smallholder farmers should address institutional challenges such as information asymmetry and the lack of credit guarantees that hinder agricultural credit access.

Genomes from Verteba cave suggest diversity within the Trypillians in Ukraine

The transition to agriculture occurred relatively late in Eastern Europe, leading researchers to debate whether it was a gradual, interactive process or a colonisation event. In the forest and forest-steppe regions of Ukraine, farming appeared during the fifth millennium BCE, associated with the Cucuteni-Trypillia cultural complex (CTCC, ~ 5000–3000 BCE). Across Europe, the Neolithisation process was highly variable across space and over time. Here, we investigate the population dynamics of early agriculturalists from the eastern forest-steppe region based on the analyses of 20 ancient genomes from the site of Verteba Cave (3935–825 cal BCE). Results reveal that the CTCC individuals’ ancestry is related to both western hunter-gatherers and Near Eastern farmers, has no local ancestry associated with Ukrainian Neolithic hunter-gatherers and has steppe ancestry. An Early Bronze Age individual has an ancestry profile related to the Yamnaya expansions but with 20% of ancestry related to the other Trypillian individuals, which suggests admixture between the Trypillians and the incoming populations carrying steppe-related ancestry. A Late Bronze Age individual dated to 980–825 cal BCE has a genetic profile indicating affinity to Beaker-related populations, detected close to 1000 years after the end of the Bell Beaker phenomenon during the third millennium BCE.

Factors Influencing the Choice of Storage Technologies by Smallholder Potato Farmers in Eastern and Southwestern Uganda

Potato is a key food and cash crop in Uganda, mainly produced by smallholder farmers in the eastern and southwestern highlands of the country. This study assessed different factors influencing the choice of storage technologies by Ugandan potato farmers. Data were collected from 240 potato farmers using structured questionnaires in key potato producing districts in eastern and southwestern Uganda. Data were analysed using descriptive statistics and the multinomial probit regression model. Results indicate that potato farmers have limited access to credit and adequate extension services. Furthermore, most of the potato production is sold immediately after harvest. Although significant quantities of potato are stored as food for the household and seed for the next season, very few farmers store ware potato for later sale at a higher price. The farmer households generally use light storage technologies designed for seed storage, while dark stores required for proper ware potato storage are rarely used. Results for factors influencing the choice of storage technologies were mixed, and the extent and direction of influence varied with technology. The predominant factors that positively influenced the choice of dark storage technologies or a combination of different storage technologies included monthly income from sources other than potato sales, access to storage management advice and access to credit. This study recommends enhancing farmers’ access to adequate extension services and credit to promote good ware potato storage conditions.

Climate shaped how Neolithic farmers and European hunter-gatherers interacted after a major slowdown from 6,100 BCE to 4,500 BCE.

The Neolithic transition in Europe was driven by the rapid dispersal of Near Eastern farmers who, over a period of 3,500 years, brought food production to the furthest corners of the continent. However, this wave of expansion was far from homogeneous, and climatic factors may have driven a marked slowdown observed at higher latitudes. Here, we test this hypothesis by assembling a large database of archaeological dates of first arrival of farming to quantify the expansion dynamics. We identify four axes of expansion and observe a slowdown along three axes when crossing the same climatic threshold. This threshold reflects the quality of the growing season, suggesting that Near Eastern crops might have struggled under more challenging climatic conditions. This same threshold also predicts the mixing of farmers and hunter-gatherers as estimated from ancient DNA, suggesting that unreliable yields in these regions might have favoured the contact between the two groups.

Socio-economic determinants and impact of adopting climate-smart Brachiaria grass among dairy farmers in Eastern and Western regions of Kenya

The sustainability of the livestock sector in sub-Saharan Africa is negatively affected by limited access to high-quality fodder in adequate quantities. The effects of climate change further exacerbate feed availability. Therefore, there is a need to develop feasible cost-effective strategies for improving the year-round feed supply. Improved planted forages such as Brachiaria grass have been recommended as one of the strategies of alleviating feed scarcity, especially in drier agro-ecological zones. This study analyses the socio-economic determinants of adoption and the impact of adopting Brachiaria grass for feed sufficiency and increased milk production. Propensity Score Matching (PSM) method was used to assess the determinants and impact of the adoption of Brachiaria grass. Empirical results indicate that the adoption of Brachiaria grass led to a significant increase in milk production by 27.6% and feed sufficiency by 31.6%. The positive impact of Brachiaria grass is consistent with the role of agricultural technologies in improving the productivity, income, and welfare of smallholder farmers. The adoption of Brachiaria grass is influenced by age of farmer, tropical livestock unit (TLU), type of animal breed, perceived benefits of the technology, access to extension, and farmer group membership. The study recommends holistic policy approaches that promote the widespread adoption of Brachiaria grass. There is also a need for an effective information dissemination pathway for Brachiaria grass.

Evaluation of Seed Quality Attributes of Sorghum Germplasm Accessions from Eastern, Coastal and Nyanza Regions, Kenya

Sorghum (Sorghum bicolor L.) is an important cereal crop in Kenya. Despite the crops importance, the yields attained by farmers in Eastern, Coastal and Nyanza regions of Kenya remain low. Access to good quality seeds of sorghum is one of the constraints facing the subsistence farmers. Good quality seed is important for increasing yield to attain food security. The aim of this study was to evaluate quality attributes of the seed used by farmers. A total of 108 germplasm accessions were obtained from 76 farmers. The seeds were tested for time and percentage of germination, seed vigour index, shoot and root dry weight. Data collected was subjected to analysis of variance. Means were separated using Fisher’s Least Significance Difference test at p ≤ 0.05. Seed samples of 26 accessions attained germination percentage below stipulated standards by Seeds and Plant Varieties Act CAP 326. Majority of seeds showed longer mean germination time with only nine accessions germinating in less than ten days. Seed vigour index was relatively high in most of the accessions, while biomass accumulation varied from high to very low among accessions. Though most of the seeds attained a high germination percentage, about 92% of seeds showed longer mean germination time. The environmental conditions in the fields, pre and post harvest handling practices impact on the seed quality hence the wide variability in germination percentage, germination time, seed vigour index and dry matter accumulation. Therefore the need to improve quality of seeds used by subsistence farmers by providing extension services on best pre and post harvest handling practices. Increasing production of sorghum in these regions will contribute significantly towards realizing food security. Further analysis could be carried out on genetic and sanitary quality aspects of the seeds planted by farmers in Eastern, Nyanza and Coastal regions.

Genetic history of Chad

The Sahel is a semi-arid zone stretching from the Atlantic Ocean in the west to the Red Sea in the east and from the Sahara in the north to the Sudanian Savanna in the south. Here, we investigated the genetic history of the spread of Northern African ancestry common among Berbers, the Y DNA haplogroup R1b-V88, and Chadic languages throughout the Sahel, with a focus on Chad. We integrated and analyzed genotype data from 751 individuals from Chad, Burkina Faso, Mali, South Sudan, and Sudan in the context of a global reference panel of 5,966 individuals. We found that genetic diversity in Chad was broadly divided by a north-south axis. The core ancestry of Southern Chadians was Central African, most closely related to Pygmies. Southern Chadians then experienced four waves of gene flow over the last 3,000 years from West-Central Africans, Eastern Africans, West-Central Africans again, and then Arabians. In contrast, Northern Chadians did not share Central African ancestry and were not influenced by the first wave of West-Central Africans but were influenced by Northern African ancestry. We found that Y DNA haplogroup R1b entered the Chadian gene pool during Baggarization. Baggara Arabs spoke Arabic, not Chadic, implying that people carrying R1b-V88 were not responsible for the spread of Chadic languages, which may have spread approximately 3,700 years ago. We found no evidence for migration of Near Eastern farmers or any ancient episodes involving Eurasian backflow.

Farmers’ use and adaptation of improved climbing bean production practices in the highlands of Uganda

Climbing beans offer potential for sustainable intensification of agriculture, but their cultivation constitutes a relatively complex technology consisting of multiple components or practices. We studied uptake of improved climbing bean production practices (improved variety, input use and management practices) through co-designed demonstrations and farmer-managed adaptation trials with 374 smallholder farmers in eastern and southwestern Uganda. A sub-set of these farmers was monitored one to three seasons after introduction. About 70% of the farmers re-planted climbing beans one season after the adaptation trial, with significant differences between eastern (50%) and southwestern Uganda (80–90%). Only 1% of the farmers used all of the improved practices and 99% adapted the technology. On average, farmers used half of the practices in different combinations, and all farmers used at least one of the practices. Yield variability of the trials was large and on average, trial plots did not yield more than farmers’ own climbing bean plots. Yet, achieved yields did not influence whether farmers continued to cultivate climbing bean in the subsequent season. Uptake of climbing beans varied with household characteristics: poorer farmers cultivated climbing beans more often but used fewer of the best-bet practices; male farmers generally used more practices than female farmers. Planting by poorer farmers resulted in adaptations such as growing climbing beans without fertilizer and with fewer and shorter stakes. Other relationships were often inconsistent and farmers changed practices from season to season. The diversity of farmer responses complicates the development of recommendation domains and warrants the development of a basket of options from which farmers can choose. Our study shows how adoption of technologies consisting of multiple components is a complicated process that is hard to capture through the measurement of an adoption rate at a single point in time.

Gains in Maize Genetic Improvement in Eastern and Southern Africa: II. CIMMYT Open‐Pollinated Variety Breeding Pipeline

Open‐pollinated varieties (OPVs) still represent a significant proportion of the maize (Zea mays L.) seed system in many countries of sub‐Saharan Africa. The International Maize and Wheat Improvement Centre (CIMMYT) has been breeding improved maize varieties for the stress‐prone environments experienced by most smallholder farmers in eastern and southern Africa for over 30 yr. Hybrid breeding is now the major focus of the CIMMYT breeding pipeline. However, OPVs are generated within the hybrid pipeline. This is the first study to document genetic gain for maize grain yield under both optimal and stress (random and managed drought, low nitrogen [N], and maize streak virus [MSV]) conditions within the CIMMYT eastern and southern African OPV breeding pipeline. Genetic gain was estimated using the slope of the regression on the year of OPV release in regional trials over a 12‐yr period (1999–2011). Open‐pollinated varieties were separated into two maturity groups, early (<70 d to anthesis) and intermediate‐late (>70 d to anthesis). Genetic gain in the early maturity group under optimal conditions, random drought, low N, and MSV was 109.9, 29.2, 84.8, and 192.9 kg ha−1 yr−1. In the intermediate‐late maturity group, genetic gain under optimal conditions, random drought, low N, and MSV was 79.1, 42.3, 53.0 and 108.7 kg ha−1 yr−1. No significant yield gains were made under managed drought stress for both maturity groups. Our results show continued improvement in OPVs for both yield potential and stress tolerance.

Chad Genetic Diversity Reveals an African History Marked by Multiple Holocene Eurasian Migrations

Understanding human genetic diversity in Africa is important for interpreting the evolution of all humans, yet vast regions in Africa, such as Chad, remain genetically poorly investigated. Here, we use genotype data from 480 samples from Chad, the Near East, and southern Europe, as well as whole-genome sequencing from 19 of them, to show that many populations today derive their genomes from ancient African-Eurasian admixtures. We found evidence of early Eurasian backflow to Africa in people speaking the unclassified isolate Laal language in southern Chad and estimate from linkage-disequilibrium decay that this occurred 4,750–7,200 years ago. It brought to Africa a Y chromosome lineage (R1b-V88) whose closest relatives are widespread in present-day Eurasia; we estimate from sequence data that the Chad R1b-V88 Y chromosomes coalesced 5,700–7,300 years ago. This migration could thus have originated among Near Eastern farmers during the African Humid Period. We also found that the previously documented Eurasian backflow into Africa, which occurred ∼3,000 years ago and was thought to be mostly limited to East Africa, had a more westward impact affecting populations in northern Chad, such as the Toubou, who have 20%–30% Eurasian ancestry today. We observed a decline in heterozygosity in admixed Africans and found that the Eurasian admixture can bias inferences on their coalescent history and confound genetic signals from adaptation and archaic introgression.

Genomic insights into the origin of farming in the ancient Near East.

We report genome-wide ancient DNA from 44 ancient Near Easterners ranging in time between ~12,000-1,400 BCE, from Natufian hunter-gatherers to Bronze Age farmers. We show that the earliest populations of the Near East derived around half their ancestry from a ‘Basal Eurasian’ lineage that had little if any Neanderthal admixture and that separated from other non-African lineages prior to their separation from each other. The first farmers of the southern Levant (Israel and Jordan) and Zagros Mountains (Iran) were strongly genetically differentiated, and each descended from local hunter-gatherers. By the time of the Bronze Age, these two populations and Anatolian-related farmers had mixed with each other and with the hunter-gatherers of Europe to drastically reduce genetic differentiation. The impact of the Near Eastern farmers extended beyond the Near East: farmers related to those of Anatolia spread westward into Europe; farmers related to those of the Levant spread southward into East Africa; farmers related to those from Iran spread northward into the Eurasian steppe; and people related to both the early farmers of Iran and to the pastoralists of the Eurasian steppe spread eastward into South Asia.

Organic agriculture in post-war Uganda: emergence of pioneer-led niches between 1986 and 1993

AbstractUganda is the largest producer of organic commodities in Africa. While most of the literature associate the start of organic agriculture in Uganda with the first certified project, no accounts exist about non-certified organic agriculture before 1993. Both in Europe and in the USA, pioneers drove non-certified organic agriculture as a response to economic, ecological and social crises. Uganda suffered two decades of civil war ending in 1986 causing multiple crises. We explore how post-war conditions influenced the emergence of organic agriculture in Uganda. We conducted individual semi-structured interviews with 12 organic agriculture experts from Central and Southwestern Uganda. Interviews were held in English using interview guides informed by a transition theoretical perspective. Interviews were tape-recorded, transcribed and analyzed using deductive and inductive coding. Our analysis shows that the degraded environment, food insecurity and economic instability after the war created a sense of urgency for the rehabilitation of livelihoods. Pioneers, including civil society activists, farmers, entrepreneurs and researchers, responded by promoting low-cost, resource-conserving technologies and agronomic practices to smallholder farmers. Economic liberalization, decentralization and institutional vacuum eased pioneers’ activities, despite facing opponents from the government and research. Through experimental learning, demonstration farms and cooperation with the Catholic Church, public extension services, researchers and international development-oriented non-governmental organizations, pioneers reached out to farmers in Eastern, Central and Southwestern Uganda. As challenging as post-war crises may be, they offer opportunities for changing development trajectories. Therefore, reconstruction and rehabilitation efforts can accommodate sustainability concerns and allow the introduction of course-changing measures in any sector.

Biomass production and forage quality of head-smut disease resistant Napier grass accessions

Napier grass, commonly known as “elephant grass”, is a major feed used for dairy production by smallholder farmers in eastern and central Africa. However, the productivity of the grass in the region is threatened by stunt and head-smut diseases. The objective of this study was to determine biomass yield and forage quality of headsmut resistant/tolerant Napier grass accessions, in the high and lowland environments of Kenya. A field study was undertaken at two contrasting environments. The first site was in the highlands at KALRO-Muguga (altitude 2,052 metres above sea level), while the second site was in the lowlands at KALRO-Katumani (altitude 1,600 metres above sea level). The study was initiated in November 2011 using canes to plant ten Napier grass accessions (ILRI numbers 16790, 16791, 16783, 18448, 16806, 16808, 16809, 16796, 16835, 16837) in separate 4 m x 4 m plots. The grasses were first harvested at 23 week after planting at both sites. Subsequent harvests occurred at intervals of eight weeks after regeneration. There were eight growth cycles from 1st November 2011 to 9th May 2013 at each site. There were differences (P<0.05) between accessions in forage dry matter production that ranged from 28.8-51.2 metric tonnes ha -1 at KALRO-Muguga and 18.1-26.7 metric tonnes ha -1 at KALROKatumani. The accessions (numbers 16783, 16796, 16806 and 16835) resistant to head-smut disease gave dry matter yields comparable to that of accession number 16791 which was the negative check. There were differences (P<0.05) between the accessions in neutral detergent fibre at both sites. There were differences (P<0.05) in nitrogen content (mean 2.6%) at KALRO-Muguga. Accession no. 16806 was confirmed resistant to head-smut disease, while accession nos. 16783, 16796 and 16835 were tolerant to head-smut disease in glasshouse screening/ molecular studies. Key Words: Kenya, Pennisetum purpureum , rain use efficiency

An overview of Conservation Agriculture in the dry Mediterranean environments with a special focus on Syria and Lebanon

Conservation Agriculture (CA), comprising minimum or no mechanical soil disturbance through no-till seeding, organic soil mulch cover, and crop diversification is now practiced on some 157 million ha worldwide, corresponding to about 11% of the global cropped land. CA adoption in the Middle-East is low compared to other regions. Lack of knowledge on CA practices and systems discourages farmers from giving up ploughing. The main reason why farmers in the Middle-East have begun to apply the no-till system has been the cost reduction in fuel, labor and machinery required for land preparation. Soil and water conservation concerns do not appear to be the main drivers in the Middle-Eastern farmers’ decision to adopt or not to adopt CA. The adoption and uptake of CA by Middle Eastern farmers has been slow but it is nonetheless occurring gradually. Collection of information and research parameters related to agricultural practices are needed for designing a suitable soil and water conservation program for sustainable production intensification. Governmental policy encouraging the adoption and spread of CA systems in the Middle-East region is certainly a necessary condition for uptake. The objective of this article is to review the current status of adoption and spread of CA in the Middle-East, focusing mainly on Syria and Lebanon, and the potential beneficial consequences that can be harnessed through CA systems under rainfed conditions in both countries. The benefits include: higher factor productivity, yield and income; improved soil properties; climate change adaptation, including reduced vulnerability to the erratic rainfall distribution; and reduction in machinery, fuel and labor costs.

Selection for High Yield and Stability among Early Maturing Greengram Genotypes

Greengram, Vigna radiata (L.) Wilczek is an important source of proteins and micronutrients to small holder farmers in eastern and northern regions of Uganda. Most of the landraces currently grown by small scale farmers are characterized by low yields and late maturity. In order to identify high yielding and stable varieties, an experiment was conducted to evaluate the yield performance of introduced early maturing genotypes from the World Vegetable Centre (AVDRC) in seven locations of Uganda. Yield performance data for the genotypes was subjected to analysis of variance (ANOVA) to test the significance of genotype × environmental interactions as well as stability analysis using the yield – stability statistic (YSi) as an aid for simultaneous selection for high yield and stability. Analyses of variance showed that genotype × environmental interactions (G×E) were significant and therefore could not be ignored. Following the detection of significant genotype × environmental interactions, yield – stability statistics (YSi) were generated and used for simultaneous selection for high yield and stability among the introduced genotypes. Yield-stability statistics (YSi), indicated that three introduced genotypes (Filsan, Sunshine, and Blackgram) in addition to the local check were stable and high yielding. These genotypes need to be assessed for farmer preferences/tastes and other quality traits in on-farm participatory trials before they can be recommended for release.

Management of Eastern Farmers Association Community Radio and Ban Huakrok Community Radio, Chon Buri Province

This research aimed to study the management of Eastern Farmers Association Community Radio and Bann Huakrok Community Radio, to investigate the community’s participation in the two community radio stations, and to seek good management methods practiced by both community radio stations. The instruments used to collect data for this qualitative research included in-depth interview,document analysis, and non-participant observation.It was found that both community radio stations employed the same method in personnel management; that is, there was an appropriate proportion of the number of staff and radio programs and type of radio programs. Regarding the budget and supply management, so the management strategies had to be active all the time. Moreover, both radio stations had similar management systems in that they had careful budget plans which could be audited. Regarding audience management, both stations provided their listeners opportunities to request songs, share information, and be interviewed on air. Since both stations’ management styles were informal, the work was flexible. Regarding the community’s participation, both stations opened widely for any participation from the radio committee to the DJs. A recommended management strategy was the dynamic strategy which consisted of both proactive and reactive tactics, focusing on two-way communication.

Ancient human genomes suggest three ancestral populations for present-day Europeans

We sequenced the genomes of a ~7,000 year old farmer from Germany and eight ~8,000 year old hunter-gatherers from Luxembourg and Sweden. We analyzed these and other ancient genomes1–4 with 2,345 contemporary humans to show that most present Europeans derive from at least three highly differentiated populations: West European Hunter-Gatherers (WHG), who contributed ancestry to all Europeans but not to Near Easterners; Ancient North Eurasians (ANE) related to Upper Paleolithic Siberians3, who contributed to both Europeans and Near Easterners; and Early European Farmers (EEF), who were mainly of Near Eastern origin but also harbored WHG-related ancestry. We model these populations’ deep relationships and show that EEF had ~44% ancestry from a “Basal Eurasian” population that split prior to the diversification of other non-African lineages.

Ancient DNA analysis of 8000 B.C. near eastern farmers supports an early neolithic pioneer maritime colonization of Mainland Europe through Cyprus and the Aegean Islands.

The genetic impact associated to the Neolithic spread in Europe has been widely debated over the last 20 years. Within this context, ancient DNA studies have provided a more reliable picture by directly analyzing the protagonist populations at different regions in Europe. However, the lack of available data from the original Near Eastern farmers has limited the achieved conclusions, preventing the formulation of continental models of Neolithic expansion. Here we address this issue by presenting mitochondrial DNA data of the original Near-Eastern Neolithic communities with the aim of providing the adequate background for the interpretation of Neolithic genetic data from European samples. Sixty-three skeletons from the Pre Pottery Neolithic B (PPNB) sites of Tell Halula, Tell Ramad and Dja'de El Mughara dating between 8,700–6,600 cal. B.C. were analyzed, and 15 validated mitochondrial DNA profiles were recovered. In order to estimate the demographic contribution of the first farmers to both Central European and Western Mediterranean Neolithic cultures, haplotype and haplogroup diversities in the PPNB sample were compared using phylogeographic and population genetic analyses to available ancient DNA data from human remains belonging to the Linearbandkeramik-Alföldi Vonaldiszes Kerámia and Cardial/Epicardial cultures. We also searched for possible signatures of the original Neolithic expansion over the modern Near Eastern and South European genetic pools, and tried to infer possible routes of expansion by comparing the obtained results to a database of 60 modern populations from both regions. Comparisons performed among the 3 ancient datasets allowed us to identify K and N-derived mitochondrial DNA haplogroups as potential markers of the Neolithic expansion, whose genetic signature would have reached both the Iberian coasts and the Central European plain. Moreover, the observed genetic affinities between the PPNB samples and the modern populations of Cyprus and Crete seem to suggest that the Neolithic was first introduced into Europe through pioneer seafaring colonization.