F1 Cultivars Research Articles

Kvalitativní charakteristiky a antioxidační vlastnosti kultivarů brokolice (Brassica Oleracea L. var. italica Plenck)

Quality traits of three broccoli cultivars, which were grown for autumn crop was determined: ‘Chevalier’ F1, ‘Milady’ F1 (cultivars of Dutch origin) and ‘Cezar’ F1 (of Polish origin). Immediately after harvest sensory quality of broccoli was evaluated, using the quantitative descriptive analysis method (QDA). In ‘brainstorming’ session there were chosen 14 attributes (profiles) concerning smell, texture, taste / flavour. Overall quality impression was also scored and hedonic test of broccoli liking was performed. Other quality traits determined were: antioxidant activity, total carotenoids content, vitamin C content, colour parameters in CIE L*a*b* system. These traits were determined separately for florets and stems of broccoli plants, both of which are usable parts of this vegetable. Results showed that investigated cultivars differed in respect of quality traits concerning sensory attributes, antioxidant activi­ty, carotenoids content, vitamin C content and colour parameters. Results showed also that florets of broccoli performed higher antioxidant activity than stems. Carotenoids content in broccoli cultivars was much higher in florets than in stems, but vitamin C content was on similar level in florets and stems of the plants. Florets and stems of all broccoli cultivars differed much in respect of colour parameters – lightness, greenness and yellowness.

Agronomic performance of onion hybrids in Baraúna, in the semi-arid region of Brazil

In the Brazilian state of Rio Grande do Norte, the significant planting of onions is relatively recent, with producers seeking alternative ways to reduce losses. As a result, it was aimed to evaluate the agronomic performance of onion hybrids in the town of Baraúna in the semi-arid region of Rio Grande do Norte. The experimental design was of randomised blocks with six replications. Treatments consisted of eight hybrids (Cronus F1, Predilata F1, Serena F1, Fortaleza F1, Mata Hari, Luana, Lambada, Don Victor) and a control cultivar (IPA 11). The following were evaluated: average plant height; average number of leaves; diameter of the pseudostem; productivity; average bulb weight; rate of survival; leaf-waxiness; cultivar cycle; commercial classification of the bulbs. The Serena F1 and Mata Hari cultivars are recommended for the northeast semi-arid region due to high productivity, early cycle and better bulb classification. The use of these cultivars may help, in the short term, to change cultivation of the onion in the state of Rio Grande do Norte.

Characterization of a novel male sterile mutant of Tagetes patula induced by heat shock

Tagetes patula L. is an annual plant belonging to the Asteraceae family. Currently, commercial F1 cultivars are produced through the use of artificial emasculation which is a very laborious procedure, particularly in view of the composite capitulum structure. To date, no male sterile line is available in this species, but the isolation of such material would provide a valuable tool for the establishment of an efficient cross-pollination system for the production of F1 hybrids. In this study, we report the characterization of a novel pollen abortion mutant of Tagetes patula which was isolated from 1,796 heat shock induced cutting seedlings. Histological and cytological observation demonstrated that the abnormal development of the microspores during the binucleate pollen stage led to pollen abortion, and this was correlated to premature tapetum degeneration. Cross hybridization experiments indicated that a recessive nuclear gene was responsible for the trait. This novel male sterile mutant of Tagetes patula appears to be an applicable material for improving the breeding efficiency of the species and may be used to significantly improve ornamental traits in F1 generations.

Identification of Plasmodiophora brassicae Wor. isolate suppressing clubroot resistance in ‘Kilaxy’ F1 white cabbage

ABSTRACT The purpose of this study was the evaluation of the pathogenicity of Plasmodiophora brassicae Wor. field isolates gathered from clubroot-infected white cabbage Brassica oleracea var. capitata L. f. alba plants grown in different areas of Poland. The virulence of seven isolates of the pathogen was tested. Breeding materials from white cabbage resistant and susceptible to P. brassicae were used as standard objects for isolate virulence screening. Cabbage seeds were infected by submerging them into a resting-spore suspension. A screening of plant resistance using a nine-degree scale based on root symptoms was carried out at the eight-week-old plantlets stage and at harvest time on afield infected by P. brassicae spores. Isolates from Pobiednik and Grębałów were defined as more virulent. The P isolate from Pobiednik also infested plants of the clubroot resistant ‘Kilaxy’ F1 cultivar, which indicated the suppression of clubroot resistance. Its reaction to the other six isolates confirmed the resistance of this cultivar. The susceptible HTM line was characterised by the highest infestation level. A difference in the susceptibility level was noticed between lines derived from those with resistant genes, i.e. Oregon123 and ‘Badger Shipper’. The Oregon123 line was less susceptible to the applied isolates. The effect of the isolates used for seed inoculation on the reaction of the dormant plants to the pathogen on the clubroot field was observed.

The generative propagation and quality of new male-sterile cauliflowers with the Ogu-INRA and Brassica nigra cytoplasm

ABSTRACT In the years of 2012-2013 male-sterile cauliflower genotypes with the Brassica nigra cytoplasm and their maintainers were tested with respect to their ability for generative propagation and the quality of agronomical traits in comparison to male-fertile inbreeds and sterile genotypes with the Ogu-INRA cytoplasm. The ability for the generative propagation for male-sterile genotypes with the B. nigra cytoplasm was diversified and lower than for the maintainer, fertile lines and lines with the Ogu-INRA cytoplasm. Generative stalks of cauliflowers with the B. nigra cytoplasm usually had a higher number of buds and flowers in comparison to the fertile genotypes and to the sterile Ogu-INRA lines. The most desired commercial characteristics, such as circular shape, compactness of curd and intermediate or strong coverage by leaves, were noticed for sterile lines with the Ogu-INRA cytoplasm and their fertile complementary lines. Three experimental F1 hybrids showed good quality in comparison to commercial F1 cultivars.

Mapping of the Gynoecy in Bitter Gourd (Momordica charantia) Using RAD-Seq Analysis

Momordica charantia is a monoecious plant of the Cucurbitaceae family that has both male and female unisexual flowers. Its unique gynoecious line, OHB61-5, is essential as a maternal parent in the production of F1 cultivars. To identify the DNA markers for this gynoecy, a RAD-seq (restriction-associated DNA tag sequencing) analysis was employed to reveal genome-wide DNA polymorphisms and to genotype the F2 progeny from a cross between OHB61-5 and a monoecious line. Based on a RAD-seq analysis of F2 individuals, a linkage map was constructed using 552 co-dominant markers. In addition, after analyzing the pooled genomic DNA from monoecious or gynoecious F2 plants, several SNP loci that are genetically linked to gynoecy were identified. GTFL-1, the closest SNP locus to the putative gynoecious locus, was converted to a conventional DNA marker using invader assay technology, which is applicable to the marker-assisted selection of gynoecy in M. charantia breeding.

Identification and inheritance of a winter squash cultivar (Cucurbita maxima) with low heptachlor epoxide uptake ability

We aimed to find a cultivar with low Heptachlor exo-epoxide (HEPX) uptake ability among popular winter squash (Cucurbita maxima) cultivars and to reveal the heredity of cultivars with low HEPX uptake ability. The cultivar with low HEPX uptake ability ‘Hokkai 1’ was a pure-line, and the inheritance of low HEPX uptake ability could be investigated using this cultivar. Furthermore, we examined the suitability of ‘Hokkai 1’ as a parental line for F1 lines or as breeding material for low HEPX uptake ability. Heptachlor epoxide uptake ability in the shoots of young winter squash plants was a good indicator to evaluate HEPX uptake ability in the fruit. The F1 cultivar ‘TC2A’ had a low HEPX uptake ability among popular Japanese winter squash cultivars. The low HEPX uptake ability of ‘TC2A’ was derived from genes contributed by ‘Hokkai 1’, a parental line of ‘TC2A’. ‘Hokkai 1’ accumulated less HEPX than ‘TC2A’ or the ancestral cultivars. ‘Hokkai 1’ will be suitable for use as breeding material for the low HEPX uptake trait. The low HEPX uptake ability was not inherited dominantly in the F1, and the inheritance was found to be quantitative after examination of the F2 lines.

Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.)

The aim of this study was to determine the androgenic capacity of some eggplant (Solanum melongena L.) varieties and breeding lines, and to establish the effectiveness of androgenesis induction in the less responsive genotypes by crossing with more responsive genotypes that have a high ability for haploid embryo formation. Flower buds containing greenish-yellow anthers were collected when the microspores were at the late uninucleate stage, which is equivalent to a bud size where petals are not visible. The anthers were kept in the dark at 35 °C for 8 days, then incubated with a 12 h light period at 25 °C for 4 days on C medium, and then transferred to solid R medium, supplemented with 30 g/L sucrose and 0.1 mg/L kinetin. Topan, Halep Karasi, and Teorem F1 varieties and 2 breeding lines [Vd-1 and Vd-2 (LS 2346)] that have a tolerance to Verticillium dahliae Kleb. were used as the plant materials during the first part of the study. Haploid embryos were obtained from Topan and Halep Karasi at the rate of 4.16% and 2.63%, respectively. The responses of Topan and Halep Karasi eggplant varieties to the anther culture were better than those of the Teorem F1 cultivar and the Vd-1 and Vd-2 lines. Because of their responses to anther culture, the Topan and Halep Karasi varieties were used as donor parents, and they were crossed with the other 3 genotypes (Teorem F1, Vd-1, and Vd-2) reciprocally. Gametic embryogenesis among hybrids was only obtained from the Topan × Teorem F1 and Teorem F1 × Topan combinations (0.87% and 2.57%, respectively). Development of the haploid embryos and plant formation occurred at the rates of 0.69% and 2.57%, respectively. This study revealed that the effectiveness of androgenesis in eggplant strongly depended on genotypes. This crossing technique could be useful for increasing the opportunity for haploid embryogenesis from genotypes that are unlikely to form haploid embryos, and crossing between the fair-responsive and poor/nonresponsive genotypes could be a good system for qualifying androgenic response in eggplant.

Inheritance of Resistance to Phytophthora Root Rot in Chili Pepper Depending on Inoculum Density and Parental Genotypes

Phytophthora capsici Leonian causes root rot and stem blight in pepper (Capsicum spp.) and is a serious threat to pepper production because of its ability to infect every root, stem, and leaf at any developmental stage. Recently, pepper F1 cultivars resistant to Phytophthora root rot have been commercially released in Korea. However, despite many studies, the inheritance of resistance remains controversial due to differences in experimental methods, including pepper materials, pathogen isolates, inoculation conditions, and evaluation methods. Our aim was to determine the inheritance of Phytophthora root rot resistance by using three different F2 populations derived from crosses between ‘CM334’ (a resistant male parent) and three Korean landraces, ‘Subicho’ ‘Daehwacho’ and ‘Chilsungcho’ (susceptible female parents), and inoculating them with three different pathogen densities (1 ¯ 10 4 , 1 ¯ 105 , and 1 ¯ 106 zoospores/ml). The distribution patterns were varied, depending upon female parental susceptibility as well as inoculum densities. For example, as the inoculum density increased, pepper survival rates decreased. In all of the inheritance analyses, one common dominant resistant gene was participated in resistance to Phytophthora root rot. In addition, we found that a complementary gene, together with the major dominant gene, was necessary for resistance at a high (10 6 ) inoculum density, based on a 9:7 (R:S) segregation ratio. This study will be helpful in developing molecular markers linked to genes that are resistant to Phytophthora root rot.

EFFECT OF SUPPLEMENTARY LIGHTING ON THE QUALITY OF TOMATO FRUIT (SOLANUM LYCOPERSICUM L.) IN AUTUMN-WINTER CULTIVATION

One of the most important factors affecting many physiological processes including the intensity of photosynthesis and thus a proper growth, development and quality of plants is light. Chemical investigations carried out for various vegetable species (lettuce, tomato cucumber) revealed that the use of LED lamps positively affected their growth and phytochemical traits. The present work aims at comparing the effect of supplementary lighting type on the chemical composition and sensory quality of tomato fruits cultivated in the autumn-winter season. Two greenhouse tomato cultivars (‘Komeett’ F1 and ‘Starbuck’ F1) were used in this experiment. Fruit quality was evaluated by the content of total sugars, dry matter, nitrate (NO3), phosphorus and potassium. Sensory analysis was carried out using the profile method (QDA). The use of a supplementary light source increased the content of total sugars in ‘Komeett’ F1 cultivar fruits by 39.4% for HPS and 17.9% when LED light was used. The dry matter content in fruits was at similar level. A lower content of nitrates in tomato fruits was observed in the combinations where plants were lighted with HPS as well as LED lamps. However, there was not a significant effect of supplementary lighting on phosphorus content in tomato fruits. Cultivating tomato with supplementary light significantly affected the fruit sensory quality. Plants supplementary lighted with HPS and LED lamps were characterized by fruit with higher flesh juiciness, sweeter taste and higher overall quality.

Comparison of qualitative traits, biological value, chemical compounds of sweet pepper fruit

Sweet pepper fruits have a high biological value, a rich content of minerals. At present, large-fruit cultivars, distinguishable by a thick pericarp wall, high processing efficiency, good growth on fields, under covers, dominate in sweet pepper cultivation. The present research has been conducted to compare the qualitative traits, chemical composition of sweet pepper fruits of the Red Knight F1 cultivar grown in a greenhouse, on open field. The evaluation of qualitative traits, biological value, chemical composition demonstrated that fruits from the greenhouse cultivation were characterized by significantly larger fruit weight, weight of edible parts as compared to those harvested from a field, although they did not differ significantly in the pericarp thickness. Fruits produced on a field had a higher ratio of the technological to total fruit weight, which proves that the cultivar is an attractive choice for field cultivation of sweet pepper, suitable for processing. Pepper fruits from a field versus the ones grown in a greenhouse contained significantly less dry matter, reducing sugars but more vitamin C. The greenhouse-grown fruits contained higher levels of nitrogen, phosphorus, potassium, calcium, magnesium than those from a field.

Quantitative trait locus analysis for days-to-heading and morphological traits in an RIL population derived from an extremely late flowering F1 hybrid of sorghum

The hybrid vigor typical of F1 cultivars is used to boost biomass production of sorghum (Sorghum bicolor (L.) Moench). The high dry-matter yielding F1 cultivar Kazetachi uniquely shows extremely late flowering and a long culm, and is greatly different from its parents. We investigated the genetic mechanisms underlying these phenotypes by quantitative trait locus (QTL) analysis of recombinant inbred lines derived from a male-fertile line and a restorer line and grown in 3 years. QTL analysis for six traits (days-to-heading, culm length, culm width, culm number, panicle length, panicle number) revealed that the unique phenotypes of the F1 plants were controlled by the genetic combination of 12 or more QTLs detected in at least 2 years. Two putative QTLs for days-to-heading (qDH1 on SBI-01 and qDH6 on SBI-06) would strongly affect the other phenotypes because of their co-localization with QTLs for other traits, as supported by significant phenotypic correlations. These QTLs would be useful for understanding the association of plant type with biomass production in sorghum.

Soğukta depolamanın şeker mısırın şeker ve kuru madde değişimine etkisi

The research was conducted with the aim to determine effects of different storage methods (Control, + 4°C and deep freeze) on total sugar amount, dry matter and some sensory characteristics (color and smell) in sweet corn at Food Engineering Laboratory of Engineering and Architectural Faculty of Suleyman Demirel University in 2010 year. In the study, Lumina F1 and Composite sweet corn obtained from Sakarya Agricultural Research Institute were used as experimental material. Dry matter content of sweet corn constantly increased in the 2, 4, 6, 8, 10 and 12th days compare to control (first day) at all storage in both cultivars. Reduction in the sugar amount of sweet corn storaged at + 4°C and deep freeze was less than the control. In the study, reduction in the sugar amount of Lumina F1 and Sakarya Composite cultivars were determined as 77.89-80.05 % in the control, 37.02 - 35.90 % in the + 4°C and 37.02 - 35.90 % in the deep freeze, respectively. There has been changed from the second day, the fourth day in control and +4°C respectively, and has not been any modification in deep freeze in grain color of sweet corn.

DETERMINING THE HETEROSIS EFFECT ON ORANGE FLESHED KIRKAGAC MELON GENOTYPES DEVELOPED BY F1 BREEDING

ISHS XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New Developments in Plant Genetics and Breeding DETERMINING THE HETEROSIS EFFECT ON ORANGE FLESHED KIRKAGAC MELON GENOTYPES DEVELOPED BY F1 BREEDING

Use of an Active Canopy Sensor and SPAD Chlorophyll Meter to Quantify Geranium Nitrogen Status

This research was conducted to investigate the potentials of normalized difference vegetation index (NDVI), a Soil-Plant Analyses Development (SPAD) chlorophyll meter, and leaf nitrogen (N) concentration [% dry matter (DM)] for rapid determination of N status in potted geraniums (Pelargonium ×hortorum). Two F1 cultivars were chosen to represent a dark-green leaf cultivar, Horizon Deep Red, and a light-green leaf cultivar, Horizon Tangerine, and were grown in a soilless culture system. All standard 6-inch (15.24-cm) pots filled with a medium received an initial top-dress application of 5 g controlled-release fertilizer (15N–9P–12K), then plants were supplemented with additional N in the form of urea at 0, 50, 100, or 200 mg·L−1 N every few days to produce plants ranging from N-deficient to N-sufficient. The NDVI readings of individual plants from a NDVI pocket sensor developed by Oklahoma State University were collected weekly until the flowering stage. Data on flower traits, including number of pedicels (NOP), number of full umbels per pot (NOFU), total number of flowers per pot (TNF), number of flowers per pedicel (NOF), and inflorescences diameter (IFD), were collected 3 months after initial fertilizer treatment. After measuring flower traits, pedicels were removed from each pot, and SPAD value, NDVI, and leaf N concentration (g·kg−1 DM) were measured simultaneously. Cultivar and N rate significantly affected all but two flower and one N status parameters studied. The coefficient of determination R2 showed that NOP, NOFU, and TNF traits were more related to the N rates and the status parameters studied for ‘Horizon Tangerine’ than for ‘Horizon Deep Red’. For the latter cultivar, NOP and TNF traits were highly related to NDVI and SPAD values than N rates and leaf N content parameters. Correlation analysis indicated that the NDVI readings (R2 = 0.848 and 0.917) and SPAD values (R2 = 0.861 and 0.950) were significantly related to leaf N content (g·kg−1 DM) between cultivars. However, sensitivity of the NDVI and chlorophyll values to N application rate in geranium was slightly less than leaf N content. Strong correlations (R2 = 0.974 and 0.979, respectively) between NDVI and SPAD values were found within cultivars. The results demonstrated NDVI and SPAD values can be used to estimate N status in geranium. Because the pocket NDVI sensor will be cheaper than the SPAD unit, it has an advantage in determining N content in potted ornamentals.

Human health sciences—From cultivation to utilization of medicinal plants

The aim of this review is to recognize the ability of plants used as food and medicine for our health care. From this point of view, we have studied economical production techniques of medicinal plants and vegetables that have physiological functions such as disease prevention, health maintenance and improvement of physical function. We revealed the suitable cultivation techniques such as the long-term freezing seed storage, and systematic and efficient seedling production of Swertia japonica in the future domestication in Japan. We have also studied the development of a new and friendly product for preparing decoction of Kampo medicine (Japanese traditional medicine) to utilize medicinal plants effectively. It was possible that the decoction of some Kampo medicines in a micro-wavable container could be carried out in substitution for a common method. This study revealed that the improvement of decoction method of Kampo medicine might contribute to conserve the energic or natural resources, especially medicinal plants of crude drugs in comparison with the conventional way. Moreover, the reevaluation of some vegetables such as Japanese radish and carrot in terms of the suitability as materials for “Yakuzen”, and creating and producing of newly low potassium tomatoes for improving the quality of life (QOL) of dialysis patients and potassium restricted patients were also studied by focusing traditional and local vegetables. Some local cultivars containing stronger flavor and taste with higher amount of functional constituents are suitable for our health care than F1 (first filial generation) cultivars. Our research will give feedback each other by cross cutting way, and human health science from the cultivation to utilization of medicinal plants and vegetables will be important and needed for our healthy and comfortable life in the future.

Extrato da alga Ascophyllum nodosum (L.) Le Jolis, no desenvolvimento vegetativo e na produção do tomateiro

Biofertilizers based on seaweed extract are a source of nutrients that can replace conventional synthetic fertilizers, allowing their use in organic agriculture. The objective of this research was to evaluate the effect of Ascophyllum nodosum (L.) Le Jolis seaweed extract on tomato (Lycopersicon esculentum) yield and vegetative development. The experiment was carried out under protected cultivation and in the field, using the Piccolo F1 cultivar. The experimental design was completely randomized in 5 × 2 × 5 factorial arrangement (five combinations of doses and frequency of seaweed extract application; two environments: greenhouse and field, with five assessment dates) for the variables: stem diameter, average number of leaves and relative chlorophyll content. For the other variables, a 5 × 2 factorial design was used (five combinations of doses and frequency of seaweed extract application; two environments); the following variables were evaluated: stem diameter, leaf relative chlorophyll content, number of leaves, average fruit weight, number of fruits, longitudinal and transverse fruit diameter, rachis length and average yield. It was possible to verify that the 0.3% seaweed extract dose under protected cultivation and in the field, applied every two weeks, increased the tomato yield, without altering the characteristics of fruits and vegetative growth.

Seed Formation Promoted by Paclobutrazol, a Gibberellin Biosynthesis Inhibitor, in pat-2 Parthenocarpic Tomatoes

Seed production in pat-2 parthenocarpic tomatoes is sufficiently low for the propagation of parthenocarpic tomato cultivars. To establish the technology to promote seed formation in pat-2 parthenocarpic tomato plants, we examined the effects of paclobutrazol, a gibberellin biosynthesis inhibitor, on seed formation. The parthenocarpic F1 cultivar ‘Renaissance’, that has the homozygote recessive gene pat-2, was treated with paclobutrazol at 0 (control), 0.2, 1, 5, or 25 mg per pot (5.1 L) by irrigation to the culture medium. With increasing application of paclobutrazol, stem diameter increased, while stem length, leaf length, leaf width, and fruit fresh weight decreased. The percentage of fruits with seeds increased with increasing levels of paclobutrazol, and it reached 100% with 1 mg/pot and higher. Seed number per fruit increased from 12 at 0 mg/pot of paclobutrazol to 52 and 74 at 1 and 5 mg/pot, respectively. To confirm the practicality of this technology, the parthenocarpic purebred strain ‘PASK-1’, the seed parent of ‘Renaissance’, was treated with 0 (control), 1, or 5 mg/pot of paclobutrazol. All flowers of ‘PASK-1’ were emasculated before flowering and crossed at flowering with pollen of the parthenocarpic purebred strain ‘PF811K’, the pollen parent of ‘Renaissance’. The percentage of fruits with seeds increased from 59% at 0 mg/pot of paclobutrazol to higher than 95% at 1 and 5 mg/pot. Seed number per fruit increased from 21 at 0 mg/pot of paclobutrazol to 45 and 46 at 1 and 5 mg/pot, respectively. From these results, we concluded that paclobutrazol promotes seed formation in pat-2 parthenocarpic tomato plants.

SEROLOGICAL AND MOLECULAR SCREENING OF PLUM POX VIRUS (PPV) IN THE F1 AND F2 ROMANIAN APRICOT GENOTYPES

Sharka (Plum pox virus - PPV) is considered one of the most devastating diseases of stone fruits in terms of agronomic impact and economic importance. The disease is very detrimental to apricot, peach and plum trees because it reduces quality and causes premature drop of fruits. The dynamics of virus infection in F1 and F2 Romanian apricot cultivars (Prunus armeniaca) were analyzed in natural infection conditions. The F1 and F2 Romanian apricot progenies were serologically tested by DAS-ELISA using PPV-D and PPV-M specific monoclonal antibodies. Molecular tests were done by RT-PCR using P1/P2 primers. PPV detection by conventional serological and molecular methods was performed during the dormant period (wintertime). This period has important drawbacks due to both false negative results (sensitivity) and also false positive results (specificity). The Romanian apricot F1 and F2 progenies evaluated were initially classified into three groups: susceptible to PPV, partially resistant to PPV, and resistant to PPV. After that, the most important genotypes were grafted onto infected peach 'GF 305' in greenhouse conditions. The goals of the work presented in this communication are the introduction and development of marker-assisted selection (MAS) for PPV resistance in F1 and F2 progenies of Romanian apricot cultivars.

The Influence of Growing Medium and Harvest Time on the Biological Value of Cherry Fruit and Standard Tomato Cultivars

The Influence of Growing Medium and Harvest Time on the Biological Value of Cherry Fruit and Standard Tomato Cultivars The objective of this study was to examine some quality traits of tomato fruits, concerning chemical composition, in relation to the type of growing medium and harvest time. Cherry (‘Dasher’ and ‘Organza’) and standard (‘Admiro’ and ‘DRW 7594’) tomato cultivars were grown in a greenhouse with controlled climate and drip fertilizing system, in three different growing media. Environment friendly media - coconut fiber and wood fiber were compared to rockwool, commonly used as a standard horticultural medium. The type of tomato cultivar as well as harvest time significantly influenced fruit quality. The highest fruit quality was obtained from red cherry ‘Dasher’ F1 cultivar in the July harvest. The content of macronutrients in tomato fruit were significantly depend on growing media. The higher content of phosphorus was obtained in tomato fruits from plants cultivated on coconut fiber and nitrates from rockwool. The content of sugars, TSS and ascorbic acid in tomato fruit mostly depended on the growing medium. The most total sugars had fruits when plant cultivated on coconut fiber. Coconut fiber, wood fiber and rockwool can be used as the alternative media for different soilless growing systems, without a significant change of the taste quality of tomatoes.