You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research1 Apr 2011751 CORPORAL SMOOTH MUSCLE RELAXATION IS AFFECTED BY ABNORMAL LIPIDS METABOLISM RELATED DOWN-REGULATING OF ATP SYNTHASE Tao Xu and Jing li Tao XuTao Xu Beijing, China, People's Republic of More articles by this author and Jing liJing li Beijing, China, People's Republic of More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.1776AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Primary risk factors of ED include: hypertension, hyperlipidemia, diabetes, coronary and peripheral vascular disease, pelvic organ damage or spinal cord injury or surgery. Our preliminary study found that as society changes in lifestyle and the popularity of metabolism disorders, in addition to the traditional view on ED that the nerve damage and vascular factors caused erectile dysfunction, the metabolic factors play more and more important roles in ED. The purpose of this article is to figure out the dysfunction of corporal smooth muscle itself with aging and metabolic disorders. Whether diastolic dysfunction and fibrosis of corporal smooth muscle cells also caused the erectile dysfunction and the possible target for intervention. METHODS leptin receptor knockout mice (db/dblepr−/−) and high-fat diet for 16 weeks mice model and it°−s control were using in our expetiments, we tested the erectile corpus cavernosum smooth muscle impulse conduction, and the key factor on energy generation were detected on the molecular biological level. RESULTS In vivo the ICP of HFD induced hyperglycemia mouse model are lower. The energy related protein ATP F1 subunit has a significant decrease compared with its control, cell cycle related protein cyclinD1 and akt has also decreased while the fatty acid synthase (FAS) upregulated. In the primary cultured human Corporal smooth muscle cell study, a 24h FFA treated significantly induced fatty acid accumulation in the cell as the oil red O staining showed, the energy and cell survival related protein decrease and the fatty acid oxidation decreased while inflammatory marker protein NFκB increased. CONCLUSIONS Diabetic mice models has erectile dysfunction, the corporal smooth muscle itself, as the impact of hyperglycimia and hyperinsulindimia appeared to decline in ATP synthase protein levels and promote corporal smooth muscle to be in inflammatory state may affect the cavernous smooth muscle structure and downregulate the energy generation which might cause erectile dysfunction. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e302 Peer Review Report Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Tao Xu Beijing, China, People's Republic of More articles by this author Jing li Beijing, China, People's Republic of More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...