Sumatran fleabane (Conyza sumatrensis) weed can be found in several different agricultural environments and impacts different crops, such as soybean and maize. This weed may have a substantial impact on soybean yield. The aim was to evaluate the interference of C. sumatrensis on the grain yield of soybean cultivars. Soybean cultivars were used with late or early maturity, under 0, 1, 2, 3, 4, 6, 8, and 10 plants m-2 of C. sumatrensis. The four trials, composed of the two cultivars in each of the growing seasons, were analyzed separately. Because differences were found to be significant using the F-test in the comparison between trials (P≤0.05). The yield was subjected to analysis of variance and F-test. A nonlinear, rectangular hyperbolic regression model was fitted. For the early maturity cultivar, infestation levels of 17.1 and 17 of plants m-2 in the 2016–2018 and 2017–2018 growing seasons, respectively, were required to cause a 50% yield loss. For late-maturity cultivars, the values were 6.3 and 7.0 in the 2016–2017 and 2017–2018 growing seasons, respectively. The yield reduction observed for the late-maturity cultivar was 12.54 and 13.72% per plant of C. sumatrensis, in the 2016–2017 and 2017–2018 growing seasons, respectively. The early maturity cultivar showed a reduction of 9.35 and 10.77% per plant, in the 2016-2017 and 2017–2018 growing seasons, respectively. Conyza sumatrensis that cannot be tolerated in soybean, because a single plant per m2 has great potential for reducing yield, from 9.35 to 13.72%.
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