(Abridged) We use high S/N spectra of 20 HII regions in the giant spiral galaxy M101 to derive electron temperatures for the HII regions and robust metal abundances over radii R = 0.19-1.25 Ro (6-41 kpc). We compare the consistency of electron temperatures measured from the [O III]4363, [N II]5755, [S III]6312, and [O II]7325 auroral lines. Temperatures from [O III], [S III], and [N II] are correlated with relative offsets that are consistent with expectations from nebular photoionization models. However, the temperatures derived from the [O II]7325 line show a large scatter and are nearly uncorrelated with temperatures derived from other ions. Our derived oxygen abundances O/H are well fitted by an exponential distribution over six disk scale lengths, from approximately 1.3 solar in the center to 1/15 solar in the outermost region studied (for solar 12 + log (O/H)=8.7). We measure significant radial gradients in N/O and He/H abundance ratios, but relatively constant S/O and Ar/O. Our abundances are systematically lower by 0.2-0.5 dex than those derived from the most widely used strong-line empirical abundance indicators. We suspect that most of the disagreement with the strong-line abundances arises from uncertainties in the nebular models that are used to calibrate the empirical scale, and that strong-line abundances derived for HII regions and emission-line galaxies are as much as a factor of two higher than the actual oxygen abundances. However other explanations, such as the effects of temperature fluctuations on the auroral line based abundances cannot be completely ruled out. These results point to the need for direct abundance determinations of a larger sample of extragalactic HII regions, especially for objects more metal-rich than solar.
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