Extract Of Whole Plant Research Articles

Antioxidant and antidiabetic profiles of two African medicinal plants: Picralima nitida (Apocynaceae) and Sonchus oleraceus (Asteraceae).

BackgroundDiabetes mellitus (DM) is a metabolic disease characterized by chronic hyperglycaemia generally associated with oxidative stress. The present study aims at evaluating the antioxidant and antidiabetic potential of methanol and hydroethanol extracts of the stem bark and leaves of Pricralima nitida and the Sonchus oleraceus whole plant respectively.MethodsThe in vitro antioxidant activity was assessed using 1,1-Diphenyl-2-picrilhydrazyl (DPPH) for free radical-scavenging properties of the extracts, and the Folin-Ciocalteu method in determining their phenol contents. The antidiabetic activity was tested in mice following streptozotocin diabetes induction, and selected oxidative stress markers (Malondialdehyde, Hydrogen peroxides and Catalase) were measured in order to evaluate the level of oxidative stress in treated animals.ResultsThe in vitro antioxidant activity using DPPH showed IC50 ranging from 0.19 ± 0.08 to 1.00 ± 0.06 mg/mL. The highest activity was obtained with the hydroethanol extracts of S. oleraceus (0.19 mg/mL and P. nitida (0.24 mg/mL). Polyphenol contents ranged from 182.25 ± 16.76 to 684.62 ± 46.66 μg Eq Cat/g. The methanol extract of P. nitida showed the highest activity, followed by the hydroethanol extract of S. oleraceus (616.89 ± 19.20 μEq Cat/g). The hydroethanol extract of whole plants (150 mg/Kg) and methanol leave extract of P. nitida (300 mg/Kg) exhibited significant antidiabetic activities with 39.40% and 38.48% glycaemia reduction, respectively. The measurement of stress markers in plasma, liver and kidney after administration of both extracts showed significant reduction in MDA and hydrogen peroxide levels, coupled with a substantial increase in catalase activity.ConclusionsThese findings suggest that S. oleraceus whole plant and P. nitida leaves possess both antidiabetic and antioxidant properties, and therefore could be used as starting point for the development of herbal medicines and/or source of new drug molecules against diabetes.

Chlojaponilactones B – E, Four New Lindenane Sesquiterpenoid Lactones from Chloranthus japonicus

AbstractReinvestigation of the AcOEt‐soluble part of the EtOH extract of whole plants of Chloranthus japonicus afforded four new lindenane‐type sesquiterpenoid lactones, chlojaponilactones B–E (1–4, resp.), together with nine known sesquiterpenoids. Their structures and relative configurations were established on the basis of extensive spectroscopic data and by comparison with the relevant literature.

Hepatoprotective activity of Marrubium vulgare against paracetamol induced toxicity

The present work was carried out to evaluate the hepatoprotective activity of Marrubium vulgare against paracetamol toxicity in Wistar rats. Hepatoprotective properties of the methanol extract of whole plant were evaluated on paracetamol induced hepatotoxicity. Hepatotoxicity was induced in albino Wistar rats by the administration of paracetamol (2 g/kg), p.o. for 7 days. Methanol extract of Marrubium vulgare was administered at the doses 100 and 200 mg/kg/day, p.o. for 7 days. Serum analysis was performed to estimate the levels of ALT, AST, ALP, albumin, total bilirubin, and triglycerides. The liver was solated and homogenized for the estimation of glutathione and malondialdehyde. Histopathology studies were also performed on the catalase liver samples. The toxic effects of paracetamol were significantly controlled in the extract treated groups which was manifested by the restoration of serum biochemical parameters to near normal levels. From the study it was concluded that M. vulgare possess significant hepatoprotective properties.

Three new phenolic glycosides from the Tibetan medicinal plant Aconitum tanguticum

Three new phenolic glycosides, such as (Z)-sinapic acid-4-O-β-d-allopyranoside (1), 3,4-dihydroxyphenethoxy-8-O-β-d-[6-O-(4-O-β-d-glucopyranosyl)-feruloyl]-glucopyranoside (2), and 4-dihydroxyphenethoxy-8-O-β-d-[6-O-(4-O-β-d-glucopyranosyl)-feruloyl]-glucopyranoside (3) were isolated from the EtOH extract of whole plant of Aconitum tanguticum (Maxim.) Stapf. The structures of the new compounds were elucidated by spectroscopic methods, and the total 1H NMR and 13C NMR chemical shifts were assigned.

Evaluation of antioxidant activity and cytotoxic potential of Cryp-tocoryne ciliata

Cryptocoryne ciliata belonging to the Araceae family has been investigated for isolation of its secondary metabolites and evaluation of biological activities of the crude extractives with special emphasis to the antioxidant activity and brine shrimp lethality bioassay. The whole plant was extracted with methanol and concentrated extract was partitioned using petroleum ether, carbon tetrachloride and ethyl acetate. Aqueous soluble fraction of the methanolic extract showed the highest antioxidant activity. The carbon tetrachloride soluble fraction of the methanolic extract and the ethyl acetate soluble fraction of the methanolic extract showed moderate antioxidant activity as compared to free antioxidant activity of tert-butyl-1-hydroxytolunene. In the brine shrimp lethality bioassay, among all extracts of whole plant of C. ciliata, the carbon tetrachloride soluble fraction of the methanolic extract showed strong cytotoxic activity. Aqueous soluble fraction of the methanolic extract, methanolic crude and ethyl acetate soluble fraction of the methanolic extract showed mild cytotoxity as compared to that of vincristine sulphate. The study confirms the mild to moderate antioxidant and moderate potent cytotoxic activities of C. ciliata plant extract as compared to reference standards and therefore demands the isolation of active principles and thorough bioassay.DOI: http://dx.doi.org/10.3329/icpj.v2i2.13196 International Current Pharmaceutical Journal 2013, 2(2): 38-41

Membrane Stabilizing And Antimicrobial Activities Of Caladium Bicolor And Chenopodium Album

The crude methanol extracts of whole plant of Caladium bicolor (Aiton) Vent. and leaf of Chenopodium album L. as well as their pet-ether, carbon tetrachloride, chloroform and aqueous soluble fractions were evaluated for membrane stabilizing and antimicrobial activities. At concentration 1.0 mg/ml, the carbon tetrachloride soluble fraction of C. bicolor inhibited 43.92±1.63% and 38.08±0.83 % hypotonic solution and heat induced haemolysis of RBCs, respectively. Among the extractives of C. album, the aqueous soluble fraction inhibited 47.11±0.49 % and 36.73±0.76 % hypotonic solution and heat induced haemolysis of RBCs as compared to 72.79 % and 42.12 % by acetyl salicylic acid (0.10 mg/ml), respectively. C. bicolor test samples demonstrated zone of inhibition ranging from 6.0 to 20.0 mm. The chloroform soluble fraction showed the highest zone of inhibition (20.0 mm) against Staphylococcus aureus. The test samples of C. album displayed zone of inhibition ranging from 7.0 to 13.0 mm. The highest zone of inhibition (13.0 mm) was showed by the chloroform soluble fraction against Salmonella paratyphi. Keywords- Caladium bicolor (Aiton) Vent., Chenopodium album L., membrane stabilizing activity, hypotonic solution, disc diffusion method, zone of inhibition

Hepatoprotective and Antioxidant Activity of Ethanol Extract of Cynoglossum zeylanicum (Vahl ex Hornem) Thurnb ex Lehm in CCl4¬ Treated Rats

Article history: Received on: 07/11/2012 Revised on: 29/11/2012 Accepted on: 09/12/2012 Available online: 30/12/2012 This study was designed to evaluate the hepatoprotective and antioxidant effect of ethanol extract of whole plant of Cynoglossum zeylanicum on CCl4 induced hepatotoxicity in rats. Activities of liver marker enzymes, SGOT, SGPT and ALP, total protein, albumin, globulin, total, conjugated and unconjugated bilirubins at an oral dose of ethanol extract of Cynoglossum zeylanicum(50, 100 and150mg/kg) showed a significant hepatoprotective effect. Regarding antioxidant activity, ethanol extract of Cynoglossum zeylanicum exhibited a significant effect showing increasing levels of SOD, CAT, GPx, GSH and GRD by reducing malondialdehyde (MDA) levels.

ANTIMICROBIAL INVESTIGATION OF AMMANIA BACCIFERA LINN. AGAINST SOME URINARY AND GASTRO-INTESTINAL TRACT INFECTION CAUSING PATHOGENS

The plant Ammania baccifera Linn., commonly known as Jangli mendi, belonging to the family Lythraceae was investigated for its antimicrobial activity against some selected urinary tract (UT) and gastrointestinal tract (GIT) infection causing pathogens and human pathogenic fungi. The ethanolic extract of the whole plant as well as its leaves, stems and roots were screened to evaluate the antibiogram pattern followed by High Performance Thin Layer Chromatography (HPTLC) study. The extracts were then screened for their antibacterial activity against some UTI and GIT infection causing pathogens viz. Staphylococcus aureus MTCC*1430, Enterococcus faecalis MTCC 2729, Escherichia coli MTCC 118, Pseudomonas aeruginosa MTCC 1035, Klebsiella pneumoniae MTCC 109, Proteus mirabilis MTCC 743, Salmonella typhi, Vibrio cholerae and antifungal activity against some human pathogenic fungi viz. Aspergillus niger MTCC 1344, Candida albicans MTCC 3017, Candida tropicalis, Candida krusei by disc diffusion assay method. The minimum inhibitory concentration (MIC) was evaluated by two fold serial dilution assay method. The HPTLC study of the ethanolic extract of whole plant revealed the presence of eight number of components in the chromatogram developed at 254 nm. The ethanolic extract of. whole plant of A. baccifera showed highest antibacterial efficiency followed by leaf, stem and root extracts respectively. However, ethanolic extracts of whole plant and leaves exhibited moderate antifungal potency followed by stem and root extracts, respectively. The results of MIC indicated that the whole plant extract inhibited P. mirabilis, K. pneumoniae, S. typhi and A. niger at a concentration of 62.5 g/ml thus exhibited broad spectrum of inhibition.

HEPATOPROTECTIVE AND ANTIOXIDANT ACTIVITY OF CANSCORA PERFOLIATA LAM (GENTIANACEAE) AGAINST CCL4 INDUCED HEPATOTOXICITY IN RATS

The aim of the study was t o investigate the hepatoprotective activity of ethanol extract of whole plant of Canscora perfoliata in CCl 4 induced hepatotoxic rats. Administration of hepatotoxins (CCl 4 ) showed significant elevation of serum SGOT, SGPT, ALP, bilirubin, conjugated, unconjugated bilirubin and lipid peroxidation. Treatment with Canscora perfoliata (150 and 300mg/kg) significantly reduced the above mentioned parameters. The plant extract also enhanced the antioxidant activity. The ethanol extract of Canscora perfoliata have significant effect on the CCl 4 induced hepatotoxicity animal models.

Effect of Cynoglossum zeylanicum (Vehl ex Hornem) Thunb. Ex Lehm on Oral Glucose Tolerance in rats

The effect of ethanol extract of whole plant of Cynoglossum zeylanicum on Oral Glucose Tolerance was determined. Ethanol extract of Cynoglossum zeylanicum were evaluated for Oral Glucose Tolerance Test (OGTT) in normal and alloxan induced diabetic rats. Oral administration of ethanol extract at doses of 100 &150mg/kg body weight shows that the extract promote glucose uptake. These results suggest that the ethanol extract of Cynoglossum zeylanicum whole plant will be useful in the treatment of impaired oral glucose tolerance.

Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L.

BackgroundThe aim of this study was to screen various solvent extracts of whole plant of Torilis leptophylla to display potent antioxidant activity in vitro and in vivo, total phenolic and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations.Material and methodsA detailed study was performed on the antioxidant activity of the methanol extract of whole plant of Torilis leptophylla (TLM) and its derived fractions {n-hexane (TLH), chloroform (TLC) ethyl acetate (TLE) n-butanol (TLB) and residual aqueous fraction (TLA)} by in vitro chemical analyses and carbon tetrachloride (CCl4) induced hepatic injuries (lipid peroxidation and glutathione contents) in male Sprague-Dawley rat. The total yield, total phenolic (TPC) and total flavonoid contents (TFC) of all the fractions were also determined. TLM was also subjected to preliminary phytochemical screening test for various constituents.ResultsThe total phenolic contents (TPC) (121.9±3.1 mg GAE/g extract) of TLM while total flavonoid contents (TFC) of TLE (60.9 ±2.2 mg RTE/g extract) were found significantly higher as compared to other solvent fractions. Phytochemical screening of TLM revealed the presence of alkaloids, anthraquinones, cardiac glycosides, coumarins, flavonoids, saponins, phlobatannins, tannins and terpenoids. The EC50 values based on the DPPH (41.0±1 μg/ml), ABTS (10.0±0.9 μg/ml) and phosphomolybdate (10.7±2 μg/ml) for TLB, hydroxyl radicals (8.0±1 μg/ml) for TLC, superoxide radicals (57.0±0.3 μg/ml) for TLM and hydrogen peroxide radicals (68.0±2 μg/ml) for TLE were generally lower showing potential antioxidant properties. A significant but marginal positive correlation was found between TPC and EC50 values for DPPH, hydroxyl, phosphomolybdate and ABTS, whereas another weak and positive correlation was determined between TFC and EC50 values for superoxide anion and hydroxyl radicals. Results of in vivo experiment revealed that administration of CCl4 caused a significant increase in lipid peroxidation (TBARS) while decrease in GSH contents of liver. In contrast, TLM (200 mg/kg bw) and silymarin (50 mg/kg bw) co-treatment effectively prevented these alterations and maintained the antioxidant status.ConclusionData from present results revealed that Torilis leptophylla act as an antioxidant agent due to its free radical scavenging and cytoprotective activity.

Anti-inflammatory and Analgesic Activities of Asteracantha longifolia Nees

The present study was designed to evaluate the analgesic and anti-inflammatory activities of the ethanol extract of whole plant of Asteracantha longifolia Nees (family Acanthaceae) in mice. The analgesic activity was determined for its central and peripheral pharmacological actions using hotplate, formalin induced pain and acetic acid-induced writhing test in mice. Anti inflammatory effects were determined by ear swelling induced by croton oil, xyleneinduced ear edema, leukocyte migration induced by carrageenan, cotton pellet-induced granuloma formation. Tramadol (10 mg/kg) and Ibuprofen (100 mg/kg) were used as reference analgesic agents. The crude ethanol extract showed a significant inhibition of ear swelling caused by croton oil and xylene in mice. The crude extract decreased leukocyte migration induced by carrageenan, also moderately inhibited the weight of granuloma induced by a cotton pellet, as well as the formalin-induced pain. The extract given by p.o. route, produced significant inhibition of abdominal constrictions caused by acetic acid. Moreover, the extract also showed moderate analgesic activity on the hot plate pain threshold in mice. These data demonstrated that the plant may contain bioactive compounds possessing anti-inflammatory and analgesic activities. DOI: http://dx.doi.org/10.3329/bpj.v15i2.12586 Bangladesh Pharmaceutical Journal 15(2): 171-176, 2012

Cardio-tonic effect of the aqueous extract of whole plant of Crataegus aronia syn: azarolus (L) on isolated Rabbit’s heart

The aim of this work is to study the effect of aqueous extract of whole plant of Crataegus aronia on the force of contraction and heart rate of isolated rabbit’s heart. Six Isolated rabbit’s hearts were perfused through aorta in a Langendorff mode. Heart rate and contractility were determined for 5 min in the presence of 8 concentrations of C. aronia aqueous extract (1, 2, 5, 10, 20, 40, 100 and 200 mg/ml) or adrenaline (0.05 mM) as control drug. The changes after each treatment were compared with their baseline values. Data were collected with the help of PowerLab data acquisition and analyzed by Labchart Pro 7 software. At all time intervals recorded, there were no significant changes in the force of contraction nor the heart rate (HR) after infusion of low concentrations of the extract (1, 2, 5 and 10 mg/ml). The maximum increase in force of contraction occurred at a dose of 40 mg/ml, while the maximum decrease in HR occurred at a dose of 20 mg/ml (P< 0.01). The highest doses of the extract (100 and 200 mg/ml) caused hardening of the heart, stopping of the perfusion fluid from entering and stopped the beating of the heart. Therefore, the aqueous extract of whole plant of C. aronia syn: azarolus (L) showed a positive inotropic and negative chronotropic effects on isolated rabbit’s heart.

Antipyretic, analgesic and anti-inflammatory activity of Viola betonicifolia whole plant.

BackgroundPyrexia, algesia and inflammation are associated with several pathological conditions. Synthetic drugs available for the treatment of these conditions cause multiple unwanted effects. Several studies are ongoing worldwide to find natural healing agents with better safety profile. The current study was thus aimed at evaluating antipyretic, analgesic and anti-inflammatory activities of the methanolic extract of whole plant of V. betonicifolia (VBME).MethodsVBME was employed to assess antipyretic activity in yeast induced hyperthermia. Analgesic profile was ascertained in acetic acid induced writhing, hot plat and tail immersion test. Nevertheless, the anti-inflammatory activity was tested in carrageenan induced paw edema and histamine induced inflammatory tests. BALB/c mice were used at test doses of 100, 200 and 300mg/kg body weight intra peritoneally (i.p).ResultsIn yeast induced pyrexia, VBME demonstrated dose dependently (78.23%) protection at 300mg/kg, similar to standard drug, paracetamol (90%) at 150mg/kg i.p. VBME showed a dose dependent analgesia in various pain models i.e. acetic acid, hot plat and tail immersion having 78.90%, 69.96% and 68.58% protection respectively at 300mg/kg. However, the analgesic action of VBME was completely antagonized by the injection of naloxone like opiate antagonists. Similarly carrageenan and histamine induces inflammation was significantly antagonized by VBME, 66.30% and 60.80% respectively at 300mg/kg.ConclusionsIt is concluded that VBME has marked antipyretic, analgesic and anti-inflammatory activities in various animal models and this strongly supports the ethnopharmacological uses of Viola betonicifolia as antipyretic, analgesic and anti-inflammatory plant.

Study of Convolvulus pluricaulis for Antioxidant and Anticonvulsant Activity

Convolvulus pluricaulis Choisy is a perennial wild herb commonly found on sandy & rocky areas under xerophytic conditions in northern India. It is a reputed drug of ayurveda and reported to posses antioxidant, brain tonic, nervine tonic, laxative and has been used in anxiety, neurosis, epilepsy, insomnia, burning sensation, oedema and urinary disorders. In the present study, methanolic extract of whole plant of Convolvulus pluricaulis Choisy was evaluated for antioxidant activity by using 1, 1-diphenyl-2-picryl- hydrazyl (DPPH) free radical scavenging model and anticonvulsant activity by using maximal electroshock seizure model. In antioxidant activity, ascorbic acid was used as standard agent while results of anticonvulsant studies were compared with phenytoin. Results of antioxidant activity have demonstrated significant free radical scavenging effect for methanolic extract of Convolvulus pluricaulis Choisy. IC50 value of methanolic extract was observed as 41.00μg/ml as compared to 2.03μg/ml of ascorbic acid. Methanolic extract of C. pluricaulis was evaluated for anticonvulsant activity at 250, 500 and 1000mg/kg. Experimental results have shown that at the dose of 500 and 1000mg/kg, C. pluricaulis didn't abolish the hind limb extension, but reduced the mean recovery time from convulsion.

Chemical and Biological Investigations of &lt;i&gt;Justicia gendarussa&lt;/i&gt; (Burm. f)

In this study, three compounds, stigmasterol (1), lupeol (2), 16-hydroxylupeol (3) were isolated from the petroleum ether soluble fraction of a methanol extract of whole plant of Justica gendarussa (Burm. f). The crude methanol extract as well as its petroleum ether, carbon tetrachloride and chloroform soluble Kupchan fractions were studied for antioxidant, antimicrobial and cytotoxic activities. Among the different fractions tested for antioxidant activity, the chloroform soluble partitionate was the most potent with IC50 value of 18.80 μg/ml as compared to tertbutyl- 1-hydroxytoluene (IC50=17.69 μg/ml). Antimicrobial screening of the different extractives was conducted by the disc diffusion method and all the fractions except chloroform soluble partitionate exhibited mild antimicrobial activity with zone of inhibition ranging from 7-10 mm. In brine shrimp lethality bioassay, the petroleum ether soluble materials demonstrated the highest toxicity with LC50 of 1.27μg /ml. This is the first report of isolation of compounds from J. gendarussa, and its antioxidant, antimicrobial and cytotoxic properties. DOI: http://dx.doi.org/10.3329/dujps.v10i1.10016 DUJPS 2011; 10(1): 53-57

Evaluation of antioxidant activity of Ammania baccifera (L.) Whole plant extract in rats

ObjectiveTo study the effect of antioxidant activity of the ethanol extract of whole plant of Ammania baccifera, Linn (EEAB). MethodsTo investigate in rats against carbon tetrachloride (CCl4) induced erythrocyte damage. Simultaneous intraperitoneal administration of the crude extracts (600 or 800 mg/kg body weight/day) with carbon tetrachloride (1ml/kg of body weight) to rats for alternate days of two weeks protected the loss of functional integrity and membrane lipid alteration in red blood cells induced by oxidative stress. ResultsEEAB inhibited the accumulation of lipid peroxidation products in the plasma as well as maintained the activities of antioxidant enzymes such as superoxide dismutase (SOD) and catalase. The extracts further had the ability to decrease the membrane fluidity induced by carbon tetrachloride. ConclusionIt can therefore be suggested that the whole plant of Ammania baccifera, Linn possess an erythrocyte protective activity against drug induced oxidative stress. These findings also provide a rationale for further studies on isolation of active principles and its pharmacological evaluation.

Evaluation of antioxidant activity of Ammania baccifera (L.) whole plant extract in rats

ObjectiveTo study the effect of antioxidant activity of the ethanol extract of whole plant of Ammania baccifera, Linn (EEAB). MethodsTo investigate antioxidant activity of EEAB against carbon tetrachloride (CCl4) induced erythrocyte damage in rats, intraperitoneal administration of the crude extracts (600 or 800 mg/kg body weight/day) with CCl4 (1 mL/kg of body weight) was injected into rats for alternate days of two weeks. The loss of functional integrity and membrane lipid alteration in red blood cells induced by oxidative stress was observed. ResultsEEAB inhibited the accumulation of lipid peroxidation products in the plasma as well as maintained the activities of antioxidant enzymes such as superoxide dismutase and catalase. The extracts further had the ability to decrease the membrane fluidity induced by CCl4. ConclusionsIt can therefore be concluded that the whole plant of Ammania baccifera Linn possesses erythrocyte protective activity against drug induced oxidative stress. These findings also provide a rationale for further studies on isolation of active principles and its pharmacological evaluation.

Chemical and biological studies of Kalanchoe pinnata (Lam.) growing in Bangladesh

ObjectiveTo isolate compounds from K. pinnata and elucidate their structures and to explore preliminary antioxidant, antimicrobial, cytotoxic and thombolytic activities of extractives of the plant. MethodsThe methanol extract of whole plant of K. pinnata has been subjected to different chromatographic separation and purification processes to isolate the secondary metabolites. The structures of the isolated compounds have been elucidated by extensive NMR studies. The free radical scavenging activity of the crude extract and its different Kupchan fractions were determined on stable radical DPPH. In vitro antimicrobial activity was determined by the disk diffusion method. Cytotoxicity screening has been performed against Artemia salina. Total phenolics content, membrane stabilizing activity and thombolytic activities were assessed by following established protocol. ResultsThe isolated compounds were identified as glut-5(6)-en-3-one, taraxerone, 3β-friedelanol, β-amyrin-3-acetate, 3,5,7,3′,5′-pentahydroxyflavone and β-sitosterol. The chloroform soluble fraction showed potent antioxidant activity of (IC50= 80.0 μg/mL) and significant cytotoxicity, while the crude extract demonstrated noticeable total polyphenol content (149.24 mg of GAE/gm of extractive), moderate membrane stabilizing activity and inhibition of clot lysis of blood. ConclusionsThe obtained results rationalize the folkloric use of the plant and can be further investigated to isolate the active compounds responsible for the biological activities.

In vitro antioxidant and anti-inflammatory activities of Angelica decursiva

Mounting evidences continue to support the involvement of oxidative/nitrosative stress and inflammation in the pathogenesis of many diseases. Plant constituents having antioxidant activities together with anti-inflammatory activities may provide better opportunities to develop anti-inflammatory agents. In view of this, we evaluated the antioxidant and antiinflammatory activities of methanolic extract of whole plants of Angelica decursiva, and its solvent soluble fractions via in vitro activities against lipopolysaccharide-induced nitric oxide (NO) production in RAW 264.7 cells, as well as in vitro scavenging activities against 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid, NO, and peroxynitrite. Among the tested fractions, the ethyl acetate fraction was found as the most active antioxidant fraction together with significant anti-inflammatory effect. From the active ethyl acetate fraction, four coumarin derivatives consisting of nodakenin, nodakenetin, umbelliferone, and umbelliferone-6-carboxylic acid, along with a phenolic compound, vanillic acid, were isolated. Among them, umbelliferone 6-carboxylic acid and vanillic acid were isolated for the first time from this plant. In all antioxidant assays, vanillic acid showed the highest antioxidant potential followed by umbelliferone 6-carboxylic acid among the isolated compounds. In the anti-inflammatory assay, umbelliferone 6-carboxylic acid exhibited the highest inhibitory activity against lipopolysaccharide-induced NO production in RAW 264.7 cells with an IC(50) value of 72.98 μg/mL. Therefore, the present study reveals the potential antioxidant and antiinflammatory activities of whole plants of A. decursiva and its constituents, mainly umbelliferone 6-carboxylic acid, which could be used in the development of therapeutic and preventive agents for oxidative stress-related inflammatory diseases.