Soybean Extract Research Articles

Total compounds of Secondary Metabolites Soy-Yamghurt Formula and Nephropathy Effect in Male White Rats

Complications of diabetes mellitus cause diabetic nephropathy. Soy-yamghurt is made from a combination of Banggai sweet potato juice and fermented soybean juice to be a functional food for diabetic nephropathy sufferers. This study aimed to analyze quantitatively the secondary metabolites contained in soy-yamghurt and to determine the effectiveness of soy-yamghurt in reducing urea and creatinine levels. This study used an experimental method with a total of 25 rats divided into 5 groups, namely normal control, negative control, soyyamghurt treatment group with a comparison of yam and soybean extract, namely F1(1:1), F2(1:2), and F3(2:1). The parameters observed were urea and creatinine levels on days 0, 7, 14, 21, and 28. The results obtained were the total levels of secondary metabolites of Alkaloid compounds F1 0.10% v/v, F2 0.01% v/v, and F3 0.01% (v/v), Flavonoid compounds F1 0.14% (v/v), F2 0.12% (v/v), F3 0.13% (v/v), Tanin compounds F1 0.27% (v/v), F2 0.26% (v/v), F3 0.14% (v/v) and saponins F1 1.15% (v/v), F2 1.22% (v/v), F3 1.25% (v/v). Administration of soy-yamghurt F2 was effective in lowering urea and creatinine levels. With an average value of 14.66 mg/dL urea and 0.40 mg/dL creatinine.

Encapsulation of Grape (Vitis vinifera L.) Pomace Polyphenols in Soybean Extract-Based Hydrogel Beads as Carriers of Polyphenols and pH-Monitoring Devices.

In this work, novel bio-based hydrogel beads were fabricated by using soybean extract as raw waste material loaded with Lambrusco extract, an Italian grape cultivar. The phenolic profile and the total amount of anthocyanins from the Lambrusco extract were evaluated before encapsulating it in soybean extract-based hydrogels produced through an ionotropic gelation technique. The physical properties of the produced hydrogel beads were then studied in terms of their morphological and spectroscopic properties. Swelling degree was evaluated in media with different pH levels. The release kinetics of Lambrusco extract were then studied over time as a function of pH of the release medium, corroborating that the acidity/basicity could affect the release rate of encapsulated molecules, as well as their counter-diffusion. The pH-sensitive properties of wine extract were studied through UV-Vis spectroscopy while the colorimetric responses of loaded hydrogel beads were investigated in acidic and basic solutions. Finally, in the framework of circular economy and sustainability, the obtained data open routes to the design and fabrication of active materials as pH-indicator devices from food industry by-products.

Soybean extract firstly used as a green corrosion inhibitor with high efficacy and yield for carbon steel in acidic medium

The soybean extract (SE) was firstly investigated for corrosion inhibition characteristics on carbon steel in sulfuric medium. The weight loss and electrochemical methods were used to research the inhibition performance and adsorption properties of SE in combination with halide ions. Results showed the SE + I - inhibitors possessed outstanding corrosion inhibition efficiency, ~98%. Furthermore, the effective compound of SE was identified to be responsible for the corrosion inhibition effect. The AFM , water contact angle and XPS measurements confirm the formation of a corrosion inhibitor film on carbon steel. Combining differential capacitance curves, corrosion inhibition mechanism was further interpreted. Good inhibition efficiency, green extract solvent, facile extraction procedure, high yield and environment sustainability, endow SE as novel corrosion inhibitor with a promising future for industrial application. ● Soybean extract (SE) was firstly utilized as an eco-friendly, promising corrosion inhibitor. ● SE exhibited excellent inhibition efficiency (~98%) in combination with I - for carbon steel in sulfuric solution. ● Characteristic adsorption of I - would make for protonated SE adsorption on positively charged carbon steel surface.

Influence of ultrasonic application on soybean aqueous extract based composite edible film: Characterization and their food application

In this study, a soybean aqueous extract-based composite film was developed by incorporating beeswax, clove essential oil, and span-20 via ultrasonication. The effect of ultrasonication power level (0–600 W) on the rheological properties of the film forming solution (FFS) and important film properties such as water vapor permeability, tensile strength, elongation at break, and opacity was evaluated. It was found that ultrasonication treatment until a power level of 400 W at a constant treatment condition of 15 min and a frequency of 20 kHz improved barrier (water vapor permeability: 2.91 × 10−10 g m/m2·Pa·s) and mechanical properties (tensile strength: 2.53 MPa). Ultrasonication considerably reduced the apparent viscosity of FFS from 1.32 Pa s (control) to 0.188 Pa s (600 W) and improved the flow behavior. The smooth and homogenous surface obtained in SEM analysis validated the enhanced properties obtained for ultrasonication treated films. Model fitting of moisture sorption data of the experiments indicated that the GAB model is best suited to predict the moisture uptake behavior of developed films at all selected temperatures (25, 35, and 45 °C). Sealed samples exhibited a tearing failure during testing with a seal strength value of 59.16 N/m. A qualitative biodegradation test resulted in the degradation of films within 12 days, making them an eco-friendlier packaging option. Application of the developed film as a packaging material for muffins showed that soybean aqueous extract-based film could maintain the moisture content and textural parameters of muffins for up to six days under ambient storage.

Immunomodulatory effects of Bacillus subtilis-fermented soybean extract in mice

ABSTRACT This study investigated the potential immunomodulatory effects of fermented soybean extract (FSE) in RAW264.7 cells and BALB/c mice. The expression of proinflammatory cytokines in RAW264.7 cells was measured after LPS stimulation in the presence or absence of FSE. Mice were orally administered 125, 250, and 500 mg/kg FSE for 21 days, and their immune response was analyzed. In RAW264.7 cells, FSE significantly reduced the expression of proinflammatory cytokines. In vivo experiments revealed significantly elevated splenocyte proliferation; neutrophil migration; phagocytic activities; expression of CD18+, CD4+, and CD8+ T cells; and expression of IL-2, IL-4, and IFN-γ. The major compounds in FSE were identified by GC–MS and by LC–MS/MS. Molecular docking analysis showed that daidzin, genistin and daidzein could potentially bind to the human high-affinity FcγRI–IgG complex to be involved in immunological responses. Taken together, FSE can be a candidate ingredient for health functional food with enhanced immunomodulating ability.

HPLC-UVD method validation for quantitative analysis of camelliaside A in hot-water extract of soybean (Glycine max L.) leaves

HPLC-UVD method validation for quantitative analysis of camelliaside A in hot-water extract of soybean (<i>Glycine max</i> L.) leaves

Identification of soybean based on Sentinel-1/2 SAR and MSI imagery under a complex planting structure

Soybeans, as the major global oil seed crops, are among the large-scale agricultural products imported into China. In this way, the accurate identification of their growing areas is the basis for agricultural decision-making and planting structure adjustment, which are of great significance to the national food security. Currently, there is insufficient research on the identification of soybean, using remote sensing, under complex agricultural planting structures. In this paper, Guoyang County, a typical soybean-producing region in the north of Anhui Province, China, was selected as the study area, and then the multi-temporal Sentinel-1/2 (S-1/2) microwave and optical multispectral data were integrated to obtain the spatial distribution of the planting areas, via a stepwise hierarchical extraction strategy. Considering the Jeffries-Matusita (JM) distance, August 18 (i.e., the early pod setting stage) was correspondingly determined as more appropriate for soybean extraction, compared with other time phases. For this purpose, a set of rules were established to eliminate the non-cropland pixels, and the total distribution of the field vegetation was derived accordingly. In total, 30 candidate features (viz. 10 spectral bands, 11 vegetation indices, 4 texture features, and 5 microwave polarization features) were selected, and their importance was evaluated based on the ReliefF algorithm. With the support of the typical cover type samples, the weight assessment method for the ReliefF feature was further bundled with three machine-learning (ML) algorithms, namely, the random forest (RF), the back-propagation neural network (BPNN), and the support vector machine (SVM), to single out the optimum subset of the features for soybean identification. The results finally revealed that the ReliefF-RF model outperformed the other two. With nine optimum features, Kappa coefficient also reached up to 0.77–0.85. Furthermore, the extraction accuracy produced by the optimum subset of the features was significantly greater than the original 10 spectral bands (Kappa coefficient of 0.75–0.84), albeit slightly lower than the effect of the total 30 features (Kappa coefficient of 0.78–0.85).

Pressurized natural deep eutectic solvents: An alternative approach to agro-soy by-products.

Soybeans are mainly used for food and biodiesel production. It is estimated that soy crops worldwide will leave about 651 million metric tons of branches, leaves, pods, and roots on the ground post-harvesting in 2022/23. These by-products might serve as largely available and cheap source of high added-value metabolites, such as flavonoids, isoflavonoids, and other phenolic compounds. This work aimed to explore green approaches based on the use of pressurized and gas expanded-liquid extraction combined with natural deep eutectic solvents (NADESs) to achieve phenolic-rich extracts from soy by-products. The total phenolic and flavonoid contents of the generated extracts were quantified and compared with conventional solvents and techniques. Pressurized liquid extraction (PLE) with choline chloride/citric acid/water (1:1:11 – molar ratio) at 120°C, 100 bar, and 20 min, resulted in an optimized condition to generate phenolic and flavonoid-rich fractions of soy by-products. The individual parts of soy were extracted under these conditions, with their metabolic profile obtained by UHPLC-ESI-QToF-MS/MS and potential antioxidant properties by ROS scavenging capacity. Extracts of soy roots presented the highest antioxidant capacity (207.48 ± 40.23 mg AA/g), three times higher than soybean extracts (68.96 ± 12.30). Furthermore, Hansen solubility parameters (HSPs) were applied to select natural hydrophobic deep eutectic solvents (NaHDES) as substituents for n-heptane to defat soybeans. Extractions applying NaHDES candidates achieved a similar yield and chromatography profile (GC-QToF-MS) to n-heptane extracts.

Foliarly Applied 24-Epibrassinolide Modulates the Electrical Conductivity of the Saturated Rhizospheric Soil Extracts of Soybean under Salinity Stress.

The accumulation of salts within the rhizosphere is a common phenomenon in arid and semi-arid regions where irrigation water is high in salts. A previous study established the ameliorative effect of foliarly applied 24-epibrassinolide (BR) on soybean under salinity stress. As a follow-up to that study, this work evaluated the effects of BR on the electrical conductivity of saturated soil extracts (ECses) under soybean exposed to salt stress. Three salinity levels (3.24, 6.06 and 8.63 dS/m) in a factorial combination with six frequencies of BR application—control, seedling, flowering, podding, seedling + flowering and seedling + flowering + podding—were the treatments, and the rhizospheric ECse was monitored from 3 to 10 weeks after the commencement of irrigation with saline water (WAST). The principal component analysis revealed that samples in saline BR treatments clustered together based on the BR application frequencies. There was a significant increase in ECse with increases in salinity and WAST. The frequent application of BR significantly reduced ECse to 5.07 and 4.83 dS/m relative to the control with 6.91 dS/m, respectively, at week 10. At 8.63 dS/m, the application of BR (seedling + flowering + podding) reduced ECse by 31.96% compared with the control. The underlining mechanism is a subject for further investigation.

Effects of Isoflavone Profiles of Thermally Treated Soybean Extracts on α-Glucosidase Inhibitory Activities

Effects of Isoflavone Profiles of Thermally Treated Soybean Extracts on α-Glucosidase Inhibitory Activities

Digestive characteristics of oil body extracted from soybean aqueous extract at different pHs

Soybean oil bodies (SOBs), a natural source of pre-emulsified oil, have important potential applications in food industry. In this study, SOBs were extracted from raw soybean milk at pH 5.0, 7.0, 8.0, 9.0 and 11.0. The pH 5.0-, 7.0- and 8.0-SOB contained extrinsic proteins (mainly 7S, 11S and γ-conglycinin) and oleosins (24 kDa, 18 kDa and 16 kDa), and pH 9.0-, 11.0-SOB only had oleosins. Extrinsic protein contents decreased from 83.9% at pH 5.0-SOB to 58.2% at pH 8.0-SOB, zeta potentials increased gradually from –21.7 mV to –14.67 mV and particle sizes decreased from 924 nm to 359 nm with increasing extraction pHs. In stomach digestion, oleosins of pH 5.0-, 7.0-, 8.0-SOB were hydrolyzed more rapidly than extrinsic proteins. All 24 kDa oleosins were hydrolyzed during 5 min, and 18 kDa oleosins were completely hydrolyzed at 30 min for all SOBs. Oleosins were hydrolyzed and produced <15 kDa pepsin-resistant peptides. In addition, some 7S and 11S of pH 5.0-, 7.0-, 8.0-SOB resisted pepsin hydrolysis. For all SOB emulsions, zeta potentials decreased and the droplets sizes increased significantly (P < 0.05) with the extension of gastric digestive time. The oil droplets of all SOB emulsions aggregated, and the coalescence of oil droplets more easily occurred in pH 9.0- and 11.0-SOB emulsions. During the intestinal phase, zeta potentials and diameters of the droplets decreased gradually, and the droplets of SOB emulsions dispersed according to the CLSM. FFA release rates of pH 5.0-, 7.0-, 8.0-SOB emulsions increased rapidly and then increased slowly, however, the release tendency of pH 9.0-, 11.0-SOB emulsions were opposite. Total FFA releases of pH 5.0-, 7.0-, 8.0-, 9.0-, 11.0-SOB emulsions were respectively 52.5%, 70.5%, 59%, 48.8%, 43.3% at 180 min. Therefore, the digestive behaviors of SOB emulsions extracted at different pHs were different. This study will provide guidance for SOB products.

Green Extraction of Annatto Seed Oily Extract and Its Use as a Pharmaceutical Material for the Production of Lipid Nanoparticles.

This work developd nanomaterials formulated from annatto seed oily extract (ASE), myristic acid (tetradecanoic acid), and their fatty acid esters. The annatto seed oily extract was obtained using only soybean oil (ASE + SO) and Brazil nut oil (ASE + BNO). The UV/VIS analysis of the oily extracts showed three characteristic peaks of the bixin molecule at 430, 456 and 486 nm. The lipid nanoparticles obtained using myristic acid and ASE + BNO or only BNO showed better results than the oil soybean extract, i.e., the particle size was <200 nm, PDI value was in the range of 0.2–0.3, and had no visual physical instability as they kept stable for 28 days at 4 °C. Lipid nanoemulsions were also produced with esters of myristic acid and ASE + BNO. These fatty acid esters significantly influenced the particle size of nanoemulsions. For instance, methyl tetradecanoate led to the smallest particle size nanoemulsions (124 nm), homogeneous size distribution, and high physical stability under 4 and 32 °C for 28 days. This work demonstrates that the chemical composition of vegetable oils and myristic acid esters, the storage temperature, the chain length of fatty acid esters (FAE), and their use as co-lipids improve the physical stability of lipid nanoemulsions and nanoparticles from annatto seed oily extract.

Further slowing down of hydrolysis of amylose heated with black soybean extract by treating with nitrite under gastric conditions

Black soybean (BSB), which contains cyanidin-3-O-glucoside (C3G) and procyanidins, is cooked with rice in Japan. The color of the cooked rice is purplish red due to the binding of C3G and reddish oxidation products of procyanidins. These components can slowdown pancreatin-induced hydrolysis of amylose more significantly than the hydrolysis of amylopectin, and can react with nitrous acid in the stomach. This manuscript deals with the effects of nitrous acid on pancreatin-induced hydrolysis of amylose heated with BSB extract. The hydrolysis of amylose heated with BSB extract was slow, and the slowdown was due to the binding of C3G/its degradation products and degradation products of procyanidins. The amylose hydrolysis was slowed down further by treating with nitrite under gastric conditions. The further slowdown was discussed to be due to the binding of the products, which were formed by the reaction of procyanidins with nitrous acid, to amylose. In the products, dinitroprocyanidins were included. In this way, the digestibility of amylose heated with BSB extract can be slowed down further by reacting with nitrous acid in the stomach.

INFLUENCE OF SUBCCRITICAL WATER EXTRACTION PARAMETERS ON THE EFFICIENCY OF PROTEIN RECOVERY FROM SOYBEAN MEAL

This work is devoted to the study of the extraction process of protein from soybean meal with subcritical water. The aim of the study is to determine the influence of parameters (temperature: , extraction duration: ( ), hydromodulus: ) of the subcritical water extraction process on the protein content in the dry matter of soybean meal extract and their rational values. To determine the rational parameters of the extraction process, an empirical research method was applied using an orthogonal compositional plan of the 2-nd order for a fractional-factorial experiment . Samples of extracts were obtained on a laboratory setup based on a high pressure reactor. In the obtained samples, the total protein content was determined by the method of catalytic oxidation with chemiluminescent detection. On the basis of the obtained results, a quadratic interpolation model was constructed that relates the total protein content in the dry matter of the extract with the extraction parameters. The analysis of the constructed model made it possible to establish the optimal extraction parameters: temperature , extraction duration . The yield of protein to the dry matter of the extract at optimal values of temperature and duration of the process monotonically increases with an increase in the hydromodulus and reaches a maximum at the hydromodulus . The results obtained can be used to improve the technology for obtaining soy protein concentrate and isolate using the subcritical water extraction method. Due to the environmental friendliness of this extraction technology, the resulting extracts can be directly used to improve the nutritional value of various food products.

Factors Affecting Soybean Crude Urease Extraction and Biocementation via Enzyme-Induced Carbonate Precipitation (EICP) for Soil Improvement

Enzyme-induced carbonate precipitation (EICP) is a new biogeotechnical ground improvement technique that uses calcium carbonate (CaCO3) formed by biochemical processes to increase soil strength and stiffness. In this paper, crude urease extracted from soybeans was employed to catalyze the precipitation of CaCO3 in sand. To optimize the urease extraction efficiency, factors affecting the soybean crude urease extraction, including the powdered soybean particle size, concentration, soaking time, and soaking temperature, were addressed. This paper also provided further insight regarding the impact of the urease activity of soybean crude extract on the chemical conversion efficiency and the biocementation performance in EICP. The findings revealed that the powdered soybean concentration and the particle size were the two most important factors affecting the urease activity of the soybean crude extract. The enzyme activity utilized in the EICP process might further lead to different reactant efficiencies of urea-CaCl2 solution, and consequently, the improvement in the physical and mechanical properties of biocemented sand. Considering the chemical conversion efficiency and the biocementation performance, 60 g/L of powdered soybean was concluded as the preferred quantity for extracting the crude urease, with an enzyme activity of 6.62 mM urea min−1. Under this condition, a chemical conversion efficiency of approximately 95% for 0.5 M urea-0.5 M CaCl2 could be obtained in merely 12 h, and the unconfined compressive strength (UCS) of the EICP-treated sand exceeded 4 MPa with a CaCO3 content of ~8%. As a high-efficient cost-effective alternative to the purified enzyme for carbonate precipitation, the soybean crude urease showed great potential for ground improvement.

Evaluation of the Effectiveness of Fermented Soybean-Lettuce Powder for Improving Menopausal Symptoms.

Menopause syndrome causes a decline in the quality of life of postmenopausal women. Hormone therapy is recommended for the treatment of menopausal syndromes. However, it has several side effects. Soybean has been safely used to relieve the symptoms of menopause. Lettuce has antidiabetic and anti-inflammatory effects and improves sleep quality. Natural nitric oxide metabolites are produced through fermentation, which increases the effectiveness of the functional substances. This study assessed the alleviation of menopausal syndrome symptoms by natural nitric oxide-containing soybean lettuce extract using the Kupperman index. This study included adult women with menopausal syndrome and a Kupperman index of ≥15. After a four-week study with 40 participants, the final analysis included 39 participants in the experimental group (n = 19) and the placebo group (n = 20). Body mass index, waist circumference, and the total cholesterol, low-density and high-density lipoprotein cholesterol, and triglyceride levels were not altered before and after treatment in both groups. There was a significant decrease in the Kupperman index after treatment in the experimental group, but no significant change was observed in the placebo group. Soybean lettuce extract alleviates menopause syndrome without any special side effects.

Anti-inflammaging effects of black soybean and black rice mixture extract by reprogramming of mitochondrial respirations in murine macrophages

• Aging exacerbates the inflammatory responses in both M1 and M2 BMDMs, for which the phenomenon was termed as inflammaging. • Mitochondrial oxygen consumption in BMDMs was increased by black rice and black bean mixture extract (BBME) in aged-mice. • Increment in oxygen consumption by BBME further drove macrophage subsets into M2-like anti-inflammatory phenotypes. Chronic, persistent, low-grade inflammation in the elderly is termed ‘inflammaging’ and is one of the main causes of age-related diseases. Although the detailed molecular mechanisms of inflammaging are poorly understood, its amelioration by modulating macrophage phenotypes is of high interest. As a dietary approach, it was previously reported that black soybean ( Rhynchosia nulubilis ) and black rice ( Oryza sativa L.) contain potent bioactive components that aid in the mitigation of several metabolic diseases. The current study investigated whether dietary intervention of black soybean and black rice mixture extract (BBME) modulates inflammaging through functional and/or phenotypic alteration of mitochondrial respiration in bone marrow-derived macrophages (BMDMs). Administration of BBME not only inhibited the expression of inflammatory mediators, but also enhanced anti-inflammatory cytokines. BBME further regulated mitochondrial respiration in M1/M2 BMDMs. These results suggest BBME as a dietary modulator for prevention or amelioration of diseases related to inflammaging.

Recent Advances in Nattokinase-Enriched Fermented Soybean Foods: A Review.

With the dramatic increase in mortality of cardiovascular diseases (CVDs) caused by thrombus, this has sparked an interest in seeking more effective thrombolytic drugs or dietary nutriments. The dietary consumption of natto, a traditional Bacillus-fermented food (BFF), can reduce the risk of CVDs. Nattokinase (NK), a natural, safe, efficient and cost-effective thrombolytic enzyme, is the most bioactive ingredient in natto. NK has progressively been considered to have potentially beneficial cardiovascular effects. Microbial synthesis is a cost-effective method of producing NK. Bacillus spp. are the main production strains. While microbial synthesis of NK has been thoroughly explored, NK yield, activity and stability are the critical restrictions. Multiple optimization strategies are an attempt to tackle the current problems to meet commercial demands. We focus on the recent advances in NK, including fermented soybean foods, production strains, optimization strategies, extraction and purification, activity maintenance, biological functions, and safety assessment of NK. In addition, this review systematically discussed the challenges and prospects of NK in actual application. Due to the continuous exploration and rapid progress of NK, NK is expected to be a natural future alternative to CVDs.

Inhibitory activity of soybean (Glycine max L. Merr.) Cell Culture Extract on tyrosinase activity and melanin formation in alpha-melanocyte stimulating Hormone-Induced B16-F10 melanoma cells.

Hyperpigmentation, which causes excessive melanin synthesis and accumulation, is an important issue in the cosmetic industry. Since compounds developed against hyperpigmentation often come with side effects such as skin irritation and contact dermatitis, new studies focus on the use of natural agents that have no side effects. In this study, it was found that the effects of soybean cell culture extract (SCE) on alpha-melanocyte-stimulating hormone (α-MSH) induced melanogenesis in B16-F10 murine melanoma cells. The cells were incubated with SCE for 48h after treatment with α‑MSH for 24h to analysis the melanin content, cellular tyrosinase activity, and gene and protein expression. SCE at 1mg/mL decreased melanin content and tyrosinase activity by 34% and 24%, respectively, compared to the α-MSH-treated group, which did not decrease cell viability. In addition, SCE (1mg/mL) downregulated the expression of tyrosinase (TYR), tyrosinase-related protein (TRP)-1, tyrosinase-related protein (TRP)-2, and microphthalmia-associated transcription factor (MITF) genes 1.5-, 1.5-, 2-, and 2-fold, respectively. Furthermore, SCE inhibited the expression of TYR, TRP1, and TRP2 by decreasing the expression of MITF, as shown in a western blot assay. This study suggests that SCE reveals dose-dependent inhibition of melanin synthesis through the suppression of tyrosinase activity as well as molecular levels of TYR, TRP1, TRP2, and MITF in B16-F10 murine melanoma cells. Therefore, SCE has the potential to be an effective and natural skin-whitening agent for application in the cosmetic industry.

A Combination of Natural Products, BenPros (Green Tea Extract, Soybean Extract and Camellia Japonica Oil), Ameliorates Benign Prostatic Hyperplasia

Benign prostatic hyperplasia (BPH) is one of the most common diseases in elderly men and causes lower urinary tract symptoms due to excessive proliferation of prostate stromal and epithelial cells. The present study investigated the improving effect of BenPros, an edible natural product mixture (green tea extract, soybean extract and camellia japonica oil), against the development of BPH in vitro and in vivo. BenPros treatment showed inhibitory ability on testosterone-induced androgen receptor, prostate-specific antigen (PSA), and 5α-reductase protein expression in LNCap-LN3 cells and anti-inflammatory effects on LPS-induced increases in interleukin-6 and tumor necrosis factor-α in RAW264.7 cells. In a testosterone propionate (TP)-induced BPH rat model, BenPros decreased the up-regulated serum 5α-dihydrotestosterone and PSA levels. Moreover, BenPros also significantly reduced PSA protein expression in prostate tissue. Furthermore, TP-induced increased expression of cyclooxygenase 2 and B-cell lymphoma 2 (Bcl-2) were reduced by BenPros, resulting in an increase in the Bcl-2/BCL2-related X ratio. These regulatory abilities of BenPros on BPH inducing markers also reduced prostate size and epithelial thickness based on histological analysis. These results indicate that BenPros has a protective ability against BPH in vitro and in vivo, and it may be a promising candidate as a functional food in regulating BPH.