Soybean Extract Research Articles

Development of a Sensitive, Easy and High-Throughput Compliant Protocol for Maize and Soybean DNA Extraction and Quantitation Using a Plant-Specific Universal Taqman Minor Groove Binder Probe.

We report the optimization of a high-throughput, compliant DNA extraction method that uses standard format 96-well plates and a commercial automated DNA purification system (ABI PRISM® 6100 Nucleic Acid PrepStation). The procedure was set up for maize and soybean, the most common GMO crops and the main ingredients of several foodstuffs, and compared with an EU-validated CTAB-based method. Optimization of the DNA extraction was achieved by applying self-prepared buffers (for DNA extraction, binding, and washing) on the PrepStation loaded with proprietary glass-fiber-coated purification plates. Quantification of extracted DNA was performed by real-time PCR using previously reported endogenous soybean lectin and maize starch synthase genes and a novel plant-specific universal TaqMan MGB probe that targets the 18S rRNA multiple copy gene. Using serial dilutions of both maize and soybean genomic DNAs, we show low PCR sensitivity and efficiency for the official TransPrep DNA extraction protocol compared to the CTAB-based one. On the other hand, using serial dilutions of a standard reference plasmid containing a 137 bp sequence cloned from the 18S rRNA plant-specific ribosomal gene, we demonstrate the high PCR sensitivity and efficiency of the optimized DNA extraction protocol setup with self-prepared buffers. The limits of detection and quantification of the 18S rDNA reiteration were consistent with the calculated values, supporting the suitability of the DNA extraction procedure for high-throughput analyses of large populations and small amounts of tissue.

Preliminary assessment of adjuvant activities of Glycine Max (L.) Merr saponin extract in BALB/c mice immunized with hepatitis B virus vaccine

Background: Vaccine adjuvants are used to increase the immunogenicity of weak antigens. Some saponins have adjuvant effects that are exerted via their immune-stimulatory effects and production of cytokines. Soybean (Glycine max (L.) Merr.) contains saponins that could provide affordable vaccine adjuvants. This study aimed to assess the effect of the saponin extracts of soybean on the immune system in BALB/c mice immunized with the hepatitis B virus (HBV) vaccine and hepatitis B surface antigen (HBsAg). Methods: Saponins were extracted from soybean meal and their presence confirmed by foam generation and Fourier-transform infrared methods. A total of 51 mice were immunized in triplicate with 50 µL of various regimens of concentrations of the extracts and either HBV vaccine or HBsAg. The plasma anti-HBsAg antibody titre was determined using an enzyme immunosorbent assay (ELISA) 14 days post-immunization. Gene expression levels of interleukin-6 (IL-6) and tumour necrosis factor alpha (TNF-α), and haematological profile of the blood samples were determined. Results: When the two immunogens were co-administered with the soybean extract, immune response was slightly higher (0.799 + 0.013 for HBsAg and 0.758 + 0.012 for vaccine) than with the Revac B vaccine used alone. However, this difference was not statistically significant (p-value > 0.467, and 0.416 respectively). Unexpectedly, mice immunized with the vaccine expressed less IL-6 levels than the untreated mice (0.603- and 1.469-fold change in transcription (FCT), respectively). Mice treated with the vaccine expressed higher TNF-α levels than the untreated group (28.84 –and 1.00 FCT respectively) while adding the extract significantly reduced the expression of TNF-α (p < 0.063). Administration of immunogens and extract reduced neutrophil (P-value = 0.027) and platelet (p-value > 0.592) counts. Conclusions: The study demonstrated that soybean extract lacked marked adjuvant activity for HBV vaccine, that HBV vaccine suppressed expression of IL-6 and promoted expression of TNF-α.

Functional Properties of Peptides in Mixed Whey and Soybean Extracts after Fermentation by Lactic Acid Bacteria

Functional Properties of Peptides in Mixed Whey and Soybean Extracts after Fermentation by Lactic Acid Bacteria

Addition of various prebiotic sources on intestinal bacteria and growth performance of broiler given microparticle protein composed-diet

The research objective was to determine how the feeding diet made of microparticle protein combined with prebiotic sources affected broilers’ intestinal bacteria and growth performance. Micronized fish meals and soybean meals were produced. Inulin, glucomannan, and soybean oligosaccharide were obtained from extracts of the dahlia tuber (DTE), porang tuber (PTE), and soybean meal (SME), respectively. A completely randomized design with four treatments (D1: 21% intact protein (control), D2: 18% microparticle protein + 1.2% DTE, D3: 18% microparticle protein + 0.3% PTE, and D4: 18% microparticle protein + 0.2% SME) and six replication (8 birds each) was assigned. Protein digestibility, H/L ratio, growth performance, lactic acid bacteria (LAB), E. coli, and other variables were exposed to ANOVA and the Duncan test with a 5% probability. Except for FCR, all parameters were substantially (p0.05) higher when prebiotic sources were included in the diet compared to the control group (D1). However, D3‘s LAB population and H/L ratio were the same compared to other treatments, and feed consumption was the same across all treatments. Supplementing a diet with microparticle protein with inulin from dahlia tubers and soy-oligosaccharide of soybean meal extracts improves growth performance, which is supported by an increase in LAB population and more easily digestible protein with a lower H/L ratio

Effect of Compound Supplement on the Bone Health of Rats with Osteoporosis Induced by Ovariectomy

Background: The World Health Organization (WHO) has recognized osteoporosis as the second most serious epidemic in the world. There are many factors in the occurrence of osteoporosis, among which amenorrhea and aging are major causes, therefore the risk of osteoporosis increases with age. Moreover, osteoporosis influences personal health and mobility, and in turn, greatly increases national healthcare expenses. It is known that chicken sternal cartilage (CSC) hydrolysate, salmon nasal cartilage extract, turmeric (Curcuma longa L.) extract, mangosteen (Garcinia mangostana) extract, soybean extract, calcium citrate, vitamin C, and vitamin D have the potential to reduce bone loss and protect bones. However, this novel combination for improving bone loss has not yet been studied or discussed. Objective: This study discussed the effect of a CSC formula containing chicken sternal cartilage hydrolysate, salmon nasal cartilage extract, turmeric extract, mangosteen extract, soybean extract, calcium citrate, vitamin C, and vitamin D on improving the osteoporosis of rats which had received ovariectomy. Their body weight, body length, serum biochemical value, bone tissue, and biomechanics were analyzed. Design: The 10-week-old female Sprague-Dawley (SD) rats used in this study were randomly divided into six groups for the experiment, including the sham group (10 rats) and five ovariectomy (OVX) groups (53 rats). Two weeks after the ovariectomy, the following doses were administered to the corresponding groups respectively every day: carboxymethyl cellulose (CMC) solvent group (represented by OVX+CMC), and CSC 339 mg/kg (represented by OVX+CSC-L), CSC 678 mg/kg (represented by OVX+CSC-M), CSC 1356 mg/kg (represented by OVX+CSC-H). The positive control (alendronate, 2.5 mg/kg represented by OVX+AD) was administered three times weekly. The rats were sacrificed 14-weeks after administration of the experimental materials. Results: According to the experimental results, in comparison to the OVX+CMC group, the OVX+CSC-L group, OVX+CSCM group, and OVX+CSC-H group could significantly reduce the N-terminal telopeptides of type I collagen, the N-telopeptide (NTX) concentration, and the osteocalcin concentration in the serum. According to the micro-computed tomography analysis, in comparison to the OVX+CMC group, the femoral bone mineral density, trabecular area percentage, and trabecular thickness of the OVX+CSC-L, OVX+CSC-M, and OVX+CSC-H groups increased significantly, and the trabecular separation decreased significantly. According to the biomechanical analysis, in comparison to the OVX+CMC group, the OVX+CSC-L, OVX+CSC-M, and OVX+CSC-H groups could increase the maximum load, hardness, absorbed energy, and Young’s modulus of the femur significantly. Conclusion: CSC can increase the bone mineral density, trabecular area percentage, and trabecular thickness significantly; it can reduce the trabecular separation, NTX and osteocalcin (OCN) concentrations in serum significantly, and it can retard the bone loss induced by ovariectomy effectively, as well as protect bones. It can be used as a mild and long-term dietary supplement; however, the result of long-term administration to the human body should be observed in the future.

Uji Efikasi Berbagai Media Agens Antagonis Paenibacillus polymyxa Terhadap Penyakit Bacterial Leaf Blight Pada Tanaman Padi (Oryza sativa)

An experiment was carried out in Margasari Village, Dawuan District, Subang Regency, from July 2020 to November 2020. The purpose of this study was to study the efficacy of various media of the antagonist agent Paenibacillus polymyxa in controlling Bacterial Leaf Blight in rice plants and to find the most effective and efficient media. to control Bacterial Leaf Blight in rice plants. The method used in this study was an experiment through field experiments, with a simple randomized block design consisting of six treatments and four replications. The experimental treatments consisted of: A (Sugar Potato Extract), B (Sweet Potato Extract), C (Sugar Cassava Extract), D (Soybean Extract), E (Sugar Rice Wash Water) and F (Control). The experimental results show that: (1) All the Paenibacillus polymyxa antagonist agent media in this experiment were effective in suppressing the intensity of the attack of Bacterial Leaf Blight (2) The more effective Paenibacillus polymyxa antagonist agent medium was treatment A (Sugar Potato Extract) which was able to suppress the intensity. attack was lower than the other treatments, so it had the most number of productive tillers and produced the highest average productivity of 8.08 tons ha-1.

The Influence of Giving Green Bean Extract and Bean Extract Soybean on Increasing Haemoglobin Levels in Trimester III Pregnant Women

Anemia is a health problem throughout the world, especially in developing countries. Iron deficiency problems often cause anemia in pregnant women because iron is needed to help the body produce new red blood cells rich in oxygen and nutrients; the impact of anemia on pregnant women is not insignificant. Mainly, pregnant women who experience anemia in pregnancy will affect the period of pregnancy, labor, and childbirth, namely miscarriage, bleeding, premature parturition, uterine inertia, prolonged delivery, uterine atony, shock, stillbirth, in the puerperium, and can even have an impact on death. Aim the study to measure the increase in hemoglobin levels in third-trimester pregnant women from the effect of green bean and soybean extracts.
 This research is a quasi-experimental research with a two-group pre-test - post-test approach. The population of this study was the third-trimester pregnant women who had their pregnancies checked at the Putri Ayu Public Health Centre. The sampling technique for this study was simple random sampling, and the sample size was 20 respondents who met the inclusion criteria. The sample used in this study is simple random sampling. Following the research hypothesis described, the statistical analysis technique used is univariate with frequency distribution and bivariate with the Wilcoxon test. Research analysis was carried out with the help of a computerized program. Based on the study's Wilcoxon test results, the green bean group value was ρ = 0.003 (CI 95%), while in the soybean group, it was ρ = 0.011. This means that mung bean extract is more influential in increasing haemoglobin levels in thirdtrimester pregnant women than soybean extract.

Effect of Soybean Extract on sFlt-1 LEVELS in Huvecs Cultures Induced by Preeclampsia Plasma

Preeclampsia is a dangerous pregnancy issue that causes hypertension at ≥20 weeks of gestation. Oxidative stress is known to play a role in the pathophysiology of preeclampsia by raising the activity of soluble fms-like tyrosine kinase 1 (sFlt-1) and causing endothelial dysfunction. As a result, antioxidants are utilized as a therapy in preeclampsia to protect the body from the impacts of free radicals. This study aims to determine the effect of soybean extract on sFlt-1 levels in HUVECs cultures exposed to preeclamptic plasma. The benefit of this study is the consideration for clinicians to provide soybean ethanol extract supplements (Glycine max) to increase the potential of antihypertensive therapy. Human umbilical vein endothelial cell cultures (HUVECs) were used in the study, which was conducted in vitro. HUVECs cultures were exposed to preeclampsia plasma and subsequently treated for 24 hours with various dosages of soybean extract. The enzyme-linked immunosorbent assay (ELISA) measured the sFlt-1 levels in each observation group. The hypothesis was tested using One-Way ANOVA analysis with SPSS version 25 software. This study found a significant difference (p <0,05) between the mean value of the positive control group and the negative control group. A significant difference was also found (p <0,05) in the mean value of sFlt-1 between the positive control group and the treatment group that was given soybean extract at doses of 17,5 ppm. Soybean extract had a significant effect on decreasing sFlt-1 levels in HUVECs cultures exposed to preeclamptic plasma. Thus, soybeans isoflavones have the potential to treat preeclampsia by reducing anti-angiogenic factors.

Pengaruh Imbangan Tepung Ubi Jalar Ungu (Ipomea batatas L) dan Sari Kedelai (Glycine max L) terhadap Karakteristik Es Krim

Ice cream is a frozen dessert with a lot of milk that everyone likes. One factor determining the quality of ice cream is the density of ice cream consisting of fat, protein, carbohydrate, vitamins and minerals. The sweet purple starch and soybean extract can be added into the dough to improve the density and characteristics of the ice cream as a whole. This research has been carried out to determine the balance of sweet purple starch and soybean extract the most appropriate to produce ice cream with the best characteristics. The method used in this research is the experimental method with the Acute Group Project consisting of five treatments of the balance of purple-striped wax and silk sari which are A (35:65:0), B (30:65:5), C (25:65:10), D (20:65:15), dan E (15:65:20). Each treatment was repeated five times. The results showed that treatment B, namely the addition of 5 grams of the sweet purple starch to the dough, produced the best characteristics of ice cream based on organoleptic tests of color, taste, texture and aroma.

An Untargeted Metabolomics Approach to Study the Variation between Wild and Cultivated Soybeans.

The differential metabolite profiles of four wild and ten cultivated soybeans genotypes were explored using an untargeted metabolomics approach. Ground soybean seed samples were extracted with methanol and water, and metabolic features were obtained using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS) in both positive and negative ion modes. The UHPLC-HRMS analysis of the two different extracts resulted in the putative identification of 98 metabolites belonging to several classes of phytochemicals, including isoflavones, organic acids, lipids, sugars, amino acids, saponins, and other compounds. The metabolic profile was significantly impacted by the polarity of the extraction solvent. Multivariate analysis showed a clear difference between wild and cultivated soybean cultivars. Unsupervised and supervised learning algorithms were applied to mine the generated data and to pinpoint metabolites differentiating wild and cultivated soybeans. The key identified metabolites differentiating wild and cultivated soybeans were isoflavonoids, free amino acids, and fatty acids. Catechin analogs, cynaroside, hydroxylated unsaturated fatty acid derivatives, amino acid, and uridine diphosphate-N-acetylglucosamine were upregulated in the methanol extract of wild soybeans. In contrast, isoflavonoids and other minor compounds were downregulated in the same soybean extract. This metabolic information will benefit breeders and biotechnology professionals to develop value-added soybeans with improved quality traits.

Detergent-stable amylase production by Paenibacillus lactis strain OPSA3 isolated from soil; optimization by response surface methodology

This study aimed to isolate thermostable, alkaliphilic, and detergent-tolerant amylase-producing bacteria. Pure isolates from environmental samples were screened on a starch-based medium (pH 11), and selected isolates were identified using cultural and molecular techniques. Product optimization studies were conducted, and secreted amylase was partially purified using 40% (w/v) saturation ammonium sulfate at 4 °C. The wash performance of concentrated amylase was analyzed. A novel isolate, Paenibacillus lactis OPSA3, was selected for further studies. The isolate produced amylase optimally when grown on banana peels and soybean extracts, which are agro-wastes. Optimization by Response surface Methodology resulted in a 2.1-fold increase in alkaliphilic amylase production. A 2.46-fold purification was achieved, with an enzyme activity yield of 79.53% and specific activity of 26.19 Umg−1. Wash performance analysis using the amylase supplemented with boiled commercial detergent (kiln®) showed good cleaning efficiency. The amylase has the potential for application as a component of green detergent.

Deep eutectic solvents as an alternative for extraction of flavonoids from soybean (Glycine max (L) Merrill) and okara: An experimental and computational approach based on COSMO-SAC model

In this study, different Deep Eutectic Solvents based on choline chloride ([Ch]Cl) with carboxylic acids, sugars, and glycerol, were investigated as alternative solvents for the extraction of flavonoids from soybean and okara. Initially, the COSMO-SAC was investigated as a tool in solvent screening for the extraction of flavonoids. Experimental validation was performed using total flavonoid analysis with the solvents that showed greater interaction with the solutes. The extracts obtained from soybean and okara using the DES [Ch]Cl:acetic acid added with 30 % water showed the highest total flavonoid content, 1.05 mg eq. of catechin/g dry soybean and 0.94 mg eq. of catechin /g dry okara, respectively. For phenolic compound extraction, [Ch]Cl: acetic acid DES extracted approximately 1.16 mg GAE/g of soybean and 0.69 mg GAE/g of okara. For antioxidant activity, soybean and okara extracts obtained with [Ch]Cl: acetic acid showed FRAP results of 0.40 mg Trolox/mL of extract and 0.45 mg Trolox/mL of extract, respectively. In addition, the isoflavones daidzein, genistein, glycitein, daidzin, genistin, and glycitin were identified and quantified in the soybean and okara extracts obtained with DES [Ch]Cl: acetic acid with 30% water, totaling 1068.05 and 424.32 µg total isoflavones/g dry sample. Therefore, The COSMO-SAC model was a useful tool in solvent screening, saving time and costs. Also, DES can be an alternative solvent for extracting flavonoids to replace conventional organic solvents, respecting current environmental and human health concerns.

The role of soybean meal extracts proteins in the inhibition of mild steel acid corrosion

In this research, the mild steel corrosion inhibition effect of three extracts from soybean meal and a soybean protein commercial sample was examined using mass loss tests, electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization (PDP). The anti-corrosive properties of soybean meal extracts were investigated normalizing the peptide/protein amount. The inhibitors were chemically characterized by spectroscopic studies and HPLC. The morphological characterization of the mild steel surface was performed by SEM. Our results demonstrated that all inhibitors showed the same inhibition efficiency if the normalization in peptide/protein amount was performed, indicating proteins/peptides as the class of molecules responsible for inhibiting corrosion. For example, for 100 mg L−1 in protein content, the inhibition efficiency was 84 and 82%, respectively, from electrochemical impedance and polarization curves results, and 89 and 93–94% after 2 and 24 h immersion time, respectively, from mass loss measurements. Polarization curves indicated that all inhibitors behave as a mixed-type inhibitor, more explicit for the cathodic branch. The SEM results suggest that all extracts promoted a protective action against corrosion of the mild steel in H2SO4 solution.

Physiochemical Study of Yoghurt-like Product from Breadfruit, Coconut and Soybean Extracts Stored at Different Temperature

Yoghurt-like products has been produced from a blend of soybean, coconut and breadfruit extract in the ratio B20:C20:S60, B20:C30:S50, B30:C20:S50 and B30:C30:S40 using Lactobacillus acidophilus on the substrates. Two sets of yoghurt-like products from these composites blend was produced and stored at refrigeration (5oC) and then at ambient temperature (29±2oC) for 4 weeks and 5 days, respectively. They were examined for changes in physicochemical properties against commercial (100% cow milk) dairy yoghurt as control. The changes in total solids (mg/L) reveals the following ranges for B20:C20:S60 (6.14 -14.00), B20:C30:S50 (4.10-13.10), B30:C20:S50 (4.02-12.04), B30:C30:S40 (3.20-12.42) and control (8.01- 20.00) and B20:C20:S60 (4.22-14.00), B20:C30:S50 (3.20-13.10), B30:C20:S50 (2.80-12.04), B30:C30:S40 (3.10-12.42) and control (8.10-20.00) for refrigeration and ambient temperature, respectively. The changes in pH ranges are as follows for B20:C20:S60 (3.80-5.40), B20:C30:S50 (3.60-5.00), B30:C20:S50 (3.60-5.00), B30:C30:S40 (3.20-4.80) and control (2.80-4.60) and B20:C20:S60 (3.90-5.60), B20:C30:S50 (3.60-5.20), B30:C20:S50 (3.40-5.00), B30:C30:S40 (3.40-4.80) and control (2.78-4.80) for refrigeration and ambient temperature, respectively. The titratable acidity (g/L) reveals the following ranges for B20:C20:S60 (0.42-1.20), B20:C30:S50 (0.32-1.00), B30:C20:S50 (0.34- 0.80), B30:C30:S40 (0.30-0.9) and control (0.50-1.40), and B20:C20:S60 (0.40-1.20), B20:C30:S50 (0.32-1.00), B30:C20:S50 (0.32-0.80), B30:C30:S40 (0.30-0.70) and control (0.52- 1.80) for refrigeration and ambient temperature, respectively. The viscosity (Ns/m2) ranges are as follows for B20:C20:S60 (300-500), B20:C30:S50 (355-470), B30:C20:S50 (350-480), B30:C30:S40 (340-475) and control (400-600), and B20:C20:S60 (340-500), B20:C30:S50 (330-470), B30:C20:S50 (315-480), B30:C30:S40 (300-480) and control (430-600) for refrigeration and ambient temperature, respectively. The non-dairy yoghurt produced showed characteristics as per with the dairy yoghurt, as they exhibited almost same pattern of changes during the storage period.

Yeast biocontrol against green mold in pear orange postharvest

The objective of this study was to evaluate the efficiency of the enzyme β-1, 3-glucanase and the mechanisms of action produced by yeasts in the biological control ofgreen mold in post-harvest oranges of the pear variety. The Fully Casualized Delineation(FCD) was used. The results were submitted to analysis of variance (ANOVA), in triplicate,compared with Tukey's test at a 5% probability level (p < 0.05), using the statisticalsoftware Sisvar. It was found that the killer activity was observed in 85.7% of the yeastsevaluated. The yeast Candida peltata URM4681 showed better production of β-1.3-glucanase, between 1.071 and 5.422 µg mL -1 . A factorial delineation 2 3 was used in amedium composed of yeast extract, soybean extract and meat extract to increaseenzymatic production, reaching an increase of 4.351 times comparing the assay of lowerand higher activity. The in situ assays showed a reduction in the number of infected fruitstreated with the enzymatic extract, being on average 78.5% when compared with thecontrol treatment (distilled water). As for sporulation, the enzymatic extract, which had anaverage of 8.4 spores, did not differ statistically from the IMZ and TBZ fungicides, both in apreventive and curative manner. Regarding the severity of the disease, it was noted thatthe fruits treated with enzymatic extract, had an average damage of 7.57 %, also notdiffering statistically from fungicides, both preventive and curative. Our studies suggestthat C. peltata URM4681, makes this a good candidate for biocontrol against green mold.

Formulation and antibacterial effectiveness of fermented soybean husk extracts from industrial waste of tempe as eco-friendly hand sanitizer gel

This study aims to determine the formulation and antibacterial effectiveness of fermented soybean seed husk extract from tempe industrial waste as an environmentally friendly hand sanitizer gel. This type of research is an experimental study with variations in fermented soybean husk extract concentration, namely 0%, 5%, 15%, 20%, 50%, and 100%. Observational data were tested by analysis of variance ANOVA at a 5% level using Co-Stat software. Suppose there is a significant difference, a further test of Honest Significant Difference (BNJ) (organoleptic test), a further test of Orthogonal Polynomial Method (MOP) (spreadability, pH, viscosity), descriptive analysis test (flavonoid test, homogeneity test, antibacterial activity test is carried out) and test the total number of germs). The results showed that the fermented extract of soybean seed husk was positive for flavonoids, characterized by a bright yellow color change when added with 10% NaOH. The concentration of fermented soybean husk extract significantly affected the hand sanitizer gel's organoleptic properties, dispersion, total pH, and viscosity. Based on the organoleptic test, the panelists preferred the clear gel color in the F1 treatment (0%) and the thick, slightly liquid gel form with a slightly scented soybean husk in the F3 treatment (10%). The concentration treatment of soybean husk fermented extract (50%) was the best treatment based on good homogeneity, total pH 4.35, viscosity 2405 cPs, following SNI No.06-2588, and spreadability of 4.47 cm which was close to hand gel. For commercial sanitizers, the diameter of the inhibition zone against Escherichia coli bacteria ATCC 2922 and Staphylococcus aureus ATCC 2923 is 14 mm and 12 mm.

In silico analysis of angiotensin-converting enzyme inhibitory compounds obtained from soybean [Glycine max (L.) Merr.

Cardiovascular diseases (CVDs) are one of the major reasons for deaths globally. The renin-angiotensin-aldosterone system (RAAS) regulates body hypertension and fluid balance which causes CVD. Angiotensin-converting enzyme I (ACE I) is the central Zn-metallopeptidase component of the RAAS playing a crucial role in maintaining homeostasis of the cardiovascular system. The available drugs to treat CVD have many side effects, and thus, there is a need to explore phytocompounds and peptides to be utilized as alternative therapies. Soybean is a unique legume cum oilseed crop with an enriched source of proteins. Soybean extracts serve as a primary ingredient in many drug formulations against diabetes, obesity, and spinal cord-related disorders. Soy proteins and their products act against ACE I which may provide a new scope for the identification of potential scaffolds that can help in the design of safer and natural cardiovascular therapies. In this study, the molecular basis for selective inhibition of 34 soy phytomolecules (especially of beta-sitosterol, soyasaponin I, soyasaponin II, soyasaponin II methyl ester, dehydrosoyasaponin I, and phytic acid) was evaluated using in silico molecular docking approaches and dynamic simulations. Our results indicate that amongst the compounds, beta-sitosterol exhibited a potential inhibitory action against ACE I.

Inflammation inhibitory activity of green tea, soybean, and guava extracts during Sars-Cov-2 infection through TNF protein in cytokine storm

Coronavirus disease is caused by the pathogen severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) known as COVID-19. COVID-19 has caused the deaths of 6,541,936 people worldwide as of September 27th, 2022. SARS-CoV-2 severity is determined by a cytokine storm condition, in which the innate immune system creates an unregulated and excessive production of pro-inflammatory such IL-1, IL-6, NF Kappa B, and TNF alpha signaling molecules known as cytokines. The patient died due to respiratory organ failure and an acute complication because of the hyper-inflammation phenomenon. Green tea, soybean, and guava bioactive substances are well-known to act as anti-inflammation, and antioxidants become prospective COVID-19 illness candidates to overcome the cytokine storm. Our research aims to discover the bioactivity, bioavailability, and protein targets of green tea, soybean, and guava bioactive compounds as anti-inflammatory agents via the TNF inhibition pathway. The experiment uses in silico methods and harnesses the accessible datasets. Samples of 3D structure and SMILE identity of bioactive compounds were retrieved from the KNApSAck and Dr Duke databases. The QSAR analysis was done by WAY2DRUG web server, while the ADME prediction was performed using SWISSADME web server, following the Lipinsky rules of drugs. The target protein and protein-protein interaction were analyzed using STRING DB and Cytoscape software. Lastly, molecular docking was performed using Autodock 4.2 and visualization with BioVia Discovery Studio 2019. The identified study showed the potential of green tea, soybean, and guava’s bioactive compounds have played an important role as anti-inflammation agents through TNF inhibitor pathway.

Spermidine content of selected dietary supplements: potential for improvement?

Dietary spermidine is emerging as a substance with promising health-promoting and healthy ageing-promoting properties. Its autophagy-inducing potential has been especially well-documented. This process is suspected to be at the root of the life-prolonging effects of fasting and in-vivo spermidine supplementation. In humans, high dietary spermidine intake is associated with a lifespan increase of 5.7 years. Numerous studies provide indications of its effects on the cardiovascular, nervous, and immune systems as well as on the formation and development of tumors. Nevertheless, no health claims are currently authorized in the EU for spermidine or spermidine-rich functional foods. When ingested through food or dietary supplements, dosage appears to play an important role in replicating the favorable effects. Currently, there are ongoing proceedings regarding the novelty of a certain manufacturing process of spermidine supplements, but many other plant extracts with high spermidine content from different sources are marketed without the need for novel food authorization. This article discusses the varying test results obtained for the spermidine concentration of popular spermidine supplements. Soybean extract seems to be slightly superior in terms of spermidine concentration, but one wheat germ-based product stood out in this analysis due to a 10 times higher spermidine concentration than comparable products.Keywords: spermidine, food supplements, novel food, botanicals, functional foods, autophagy, spermidine-rich wheat germ extract

Structural and Functional Changes in Soybean Protein via Remote Plasma Treatments.

To the best of our knowledge, few studies have utilized cold plasma to improve soybean protein extraction yield and the functional properties of soybean protein. In this study, we aimed to assess the benefits of remote plasma treatments on soybean with respect to the utilization of soybean protein. This study involved two different sample forms (whole and crushed beans), two different plasma chemistry modes (ozone and nitrogen oxides [NOx = NO + NO2]), and a novel pressure-swing reactor. Crushed soybeans were significantly affected by NOx-mode plasma treatment. Crushed soybeans treated with NOx-mode plasma had the best outcomes, wherein the protein extraction yield increased from 31.64% in the control to 37.90% after plasma treatment. The water binding capacity (205.50%) and oil absorption capacity (267.67%) of plasma-treated soybeans increased to 190.88% and 246.23 % of the control, respectively. The emulsifying activity and emulsion stability slightly increased compared to those of the control. The secondary structure and surface hydrophobicity were altered. The remote plasma treatment of crushed soybeans increased soybean protein extraction yield compared to plasma-treated whole beans as well as untreated beans and altered the structural and physicochemical properties of soybean proteins.