Plant Extracts Research Articles

Histological Investigations on Potato Tuber Moths, Phthorimaea operculella Treated with a Botanical Extract of Basil (Ocimum basilicum L), with Particular Attention to the Alterations in the Ultrastructure of the Larval Digestive Tract

Background: Phthorimaea operculella (Zeller), the potato tuber moth (PTM) is one of the most important pests that attacks potato, Solanum tuberosum L. crops worldwide. Adult moths attack tubers in fields as well as in storage. Infested tubers become unsuitable for trade or consumption, the tuber damage increases gradually after several generations of the pest during storage time. Botanicals have been found to be an efficient control method especially when they act as antifeedant or repellent, or even stomach poisons. The estimation of the mortal impact of some extracts against PTM was examined by emphasizing the Ultrastructure changes in the larval digestive tract by using scanning and transmission electron microscope. Results: Structural changes throughout the entire digestive tract (Fore-, Mid- and Hind-gut), induced by Basil extract were detected through both microscope, these changes leading lastly to the death of the treated larvae. Many different ruptures and malformations were detected throughout all the digestive tract assessment. Conclusions: Studies of the occurred changes revealed some histopathological changes through out all the digestive tract (Fore-, Mid- and Hind-gut), the attached muscular layer, tracheal system, and visceral fat bodies of the larvae when fed with tubers treated with Basil extract. From this findings, the use of plant extracts can be recommended for protection of potato tuber against the infestation by the tubers moths.

Phytochemical Profiling and In vitro Antioxidant Activity of Methanolic Extracts from Fadogia cienkowskii Leaves

This study assessed the phytochemicals in the methanolic extract of Fadogia cienkowskii leaves and ascertained their antioxidant qualities In vitro, taking into account the plant's extensive usage in traditional medicine. Standard procedures were followed in the qualitative and quantitative phytochemical examination as well as the In vitro antioxidant activities of the extracts. The methanolic extracts of F. cienkowskii leaves produced a yield of 4%. The main phytochemical components found in the plant were phenolics (tannins and flavonoids), saponins, protein, and carbohydrates. The amount of total phenols in the dried plant extract was 2.085± 0.35 μg garlic acid equivalent (GAE)/mg, tannins in the extract was 0.755±0.17 μg GAE/mg, flavonoid contents in the extract was 3.646± 0.45 μg GAE/mg, and flavonols in the extract was 0.868± 0.20μg quercetin equivalent/mg. At a dosage of 10,000μg/ml, the extract demonstrated a noteworthy dose-dependent ability to scavenge DPPH radicals, effectively blocking 96.56 ± 0.46 % of DPPH, in contrast to ascorbic acid, which inhibited 89.10 ± 3.73 % at the same concentration. At a concentration of 2500μg/ml of extract, the extract demonstrated the highest level of superoxide anion (O2) inhibitory action, at 71.91±0.66% of the radical, in comparison to quercetin, which exhibited 68.23 ± 0.41% at 250μg/ml. The extract's ability to scavenge Nitric Oxide (NO) was concentration-dependent, with 250µg/ml of it being more effective than α-tocopherol. These results consequently imply that the leaf extract of F. cienkowskii likely mediates its medicinal potentials through the efficient scavenging of free radicals. Therefore, the plant can be used to create safe and natural antioxidant compounds because it has the potential to scavenge free radicals and contains sufficient concentrations of phytochemicals.

Phenolic content, antioxidant activity, and tyrosinase inhibitor potential for both skin and physical health: test of extract, skin content and content of Garcinia Cowa fruit

Skin aging leads to reduced structural integrity and reduced skin function. Medicinal plant extracts that have antioxidant properties and are able to inhibit tyrosinase have become a trend in various uses for anti-aging. One of the commonly used plants in traditional medicine that grows in many Asian countries is garcinia cowa. Currently, no one has studied the fruit from two different aspects, namely for the skin and to help in physical improvement. The purpose of this study is to test the phenolic content, antioxidants, and tyrosinase inhibition activity of the extract from the skin and pulp of garcinia cowa as well as its effectiveness on the physical ability and function of the skin. The stages carried out start from the collection of garcina cowa fruits, the determination of total phenolic levels, the determination of antioxidant activity, and the determination of tyrosinase inhibition activity. The total phenolic content was determined by the folin-ciocalteu method. The phenolic content of garcinia cowa fruit peel extract is 17.789 mg of gallic acid equivalent extract (GAE)/g, while the meat extract is 12.078 mg GAE/g extract. Furthermore, the flesh of garcinia cowa showed weak antioxidant activity with an IC50 value of 490.2 μg/mL, but the fruit peel extract showed moderate antioxidant activity at 243.3 μg/mL. The skin of garcinia cowa fruit shows tyrosinase inhibition activity in vitro, which is more significant than the pulp. Garcinia cowa peel and pulp extracts both contain phenolic compounds and show the antioxidant activity, but it was found that the fruit peel extract has a higher content. This means that with its antioxidant content, it can ward off free radicals, prevent cell damage, and reduce the risk of chronic diseases. The combination of phenolic content, antioxidant activity, and tyrosinase inhibitor potential provides significant benefits for skin health and overall physical health.

Gingerol acts as a potent radiosensitizer in head and neck squamous cell carcinoma

Treatment options for advanced head and neck squamous cell carcinoma (HNSCC) are limited and often cause severe toxicity and debilitating long-term impacts. Developing effective and safer treatments is warranted. Several plant extracts have shown their effectiveness, but a comprehensive comparison between plant extracts in HNSCC has not been reported. Our aim was to investigate the effect of different plant extracts on the proliferation and viability of HNSCC cell lines. In addition, we investigated the efficacy of combining cytotoxic plant extracts with radiation. Since RT is a cornerstone in the treatment and management of HNSCC, it is desirable to enhance its efficacy through combination with cytotoxic agents that have minimal side effects. HNSCC cell lines were treated with various plant extracts at different concentrations. MTT assays were performed to identify the most potent anti-tumor plant extract. Colony-formation assays were performed to determine the radiosensitization effect. To investigate the effect on migration, transwell migration assays were performed. Annexin V staining was performed to analyze cell apoptosis. 6-gingerol resulted in the most significant dose-dependent inhibition in all cell lines compared to other plant extracts. Colony-formation assays showed a significant radiosensitizing effect when 6-gingerol was combined with radiation. In addition, the combination of 6-gingerol with radiation resulted in a significant decrease in HNSCC cell migration. Mechanistically, Annexin V staining showed that the combination of 6-gingerol and RT induces a synergistic apoptotic effect in MOC1, MOC2 and SCC9 cells compared to RT alone. In conclusion, 6-gingerol enhances the effect of radiation in HNSCC cell lines and could be a suitable candidate for combination therapy in HNSCC.

Plant-based milk substitutes as probiotic vehicles: Health effect and survival, a review

The use of plant extracts or nondairy milk alternatives as a substitute for Cow's milk in the formulation of foods that function as vehicles for the consumption of probiotics has become popular. This substitution aims to formulate food products without lactose, low in fat, and, therefore, low in calories. Additionally, they can enter vegan or vegetarian markets. The use of these vegetable extracts has been explored for the development of probiotic foods that confer health benefits without cow milk allergy. However, few studies support that replacing Cow's milk in these foods allows probiotics to remain viable during storage, survive the digestive tract sufficiently, and reach their colonization site. Additionally, even fewer clinical studies in human or animal model assays have analyzed the impact of probiotic foods formulated with plant extracts.

Hydroalcoholic Extract of Fumaria Parviflora L. Can Potentially Detoxificate HepG2 Cell Line Following Carbon Tetrachloride Exposure

Background: Fumaria Parviflora L. (FP) is a well-known herb in Iranian traditional medicine, widely used for gastrointestinal diseases. Additionally, the detrimental effects of carbon tetrachloride (CT) on the liver and kidneys have been scientifically established. Objectives: This study was designed to assess the therapeutic effects of FP on HepG2 cells exposed to CT toxicity. Methods: After treatment with hydroalcoholic extract of the FP plant and/or CT, cell viability (using the MTT technique and lactate dehydrogenase assay) and apoptosis (using the diphenylamine assay and Annexin V/PI staining) were investigated. The ratio of BCL2/BAX mRNA expression levels was measured using real-time PCR. Data were analyzed using SPSS software. Results: A significant (P < 0.05) reduction in cell viability was observed at doses of 100 and 200 µg/mL of FP in HepG2 cells. The decreased survival rate following CT exposure was concentration-dependent and significant (P < 0.05). The IC50 of CT was determined to be 1.05 μg/mL for 24 hours. Effective detoxification associated with CT was found at 6.25 µg/mL in both the pre-treatment and simultaneous treatment groups. Cell apoptosis was significantly (P < 0.05) decreased following FP administration in CT-treated cells, with an increase in the ratio of BCL2/BAX mRNA expression levels. Conclusions: The hydroalcoholic extract of FP demonstrated hepatoprotective effects on CT-treated HepG2 cells.

Effects of dynamised high dilutions and vegetal extract based on silicon on the growth and induction of resistance in tomato plants against Rhizoctonia solani

ABSTRACT The objective of this study was to study the response of tomato (Solanum lycopersicum) against Rhizoctonia solani using dynamised high dilutions and plant extract based on silicon. The treatments were Silicea terra (ST), Thuya occidentalis (TO), and Equisetum arvense (EA) at 30CH (Centesimal Hahnemannian dilution order), Equisetum arvense leaf extract (LE), and non-dynamised water as the control. Seeds of tomato cv Marmande were treated overnight. The in vitro bioassays involved assessment of R. solani mycelial growth, seed germination, and seedling development. In the greenhouse experiment, the emergence, root and aerial part development, and disease development were evaluated. Leaves were harvested to analyse concentrations of flavonoids, phenolic compounds, chlorophyll, carotenoids, and chitinase activity. In the in vitro bioassay, LE reduced mycelial growth and TO increased the seedling vigour index, dry weight, and length of seedlings. In the greenhouse experiment, TO and ST increased plant emergence. Tomato plants treated with ST improved the fresh weight and the length of the roots. The disease severity was decreased by ST, LE, and TO. The flavonoid concentration was higher in plants treated with TO and LE. Overall, the treatments of TO, EA, and ST improved the content of phenolic compounds. Moreover, endochitinase and chitobiosidase were activated by LE.

Effect of Bio-Herbicide Application on Durum Wheat Quality: From Grain to Bread Passing through Wholemeal Flour

Using plant extracts to replace traditional chemical herbicides plays an essential role in sustainable agriculture. The present work evaluated the quality of durum wheat cv Valbelice in two years (2014 and 2016) using plant aqueous extracts of sumac (Rhus coriaria L.) and mugwort (Artemisia arborescens L.) as bio-herbicides on the main quality characteristics of durum wheat. The untreated, water-treated, and chemically treated durum wheat products were also analyzed as controls. Following the official methodologies, grain commercial analyses and defects of the kernels were determined. The main chemical and technological features were determined on the wholemeal flour: proteins, dry matter, dry gluten, gluten index, colorimetric parameters, mixograph, falling number, and sedimentation test in SDS. An experimental bread-making test was performed, and the main parameters were detected on the breads: bread volume, weight, moisture, porosity, hardness, and colorimetric parameters on crumb and crust. Within the two years, grain commercial analyses of the total five treatments showed no statistically significant differences concerning test weight (range 75.47–84.33 kg/hL) and thousand kernel weight (range 26.58–35.36 kg/hL). Differently, significant differences were observed in terms of kernel defects, particularly starchy kernels, black pointed kernels, and shrunken kernels, mainly due to the year factor. Analyses on the whole-grain flours showed significant differences. This affected dry gluten content (7.35% to 16.40%) and gluten quality (gluten index from 6.44 to 45.81). Mixograph results for mixing time ranged from 1.90 min to 3.15 min, whilst a peak dough ranged from 6.83 mm to 9.85 mm, showing, in both cases, statistically significant differences between treatments. The falling number showed lower values during the first year (on average 305 s) and then increased in the second year (on average 407 s). The sedimentation test showed no statistically significant differences, ranging from 27.75 mm to 34.00 mm. Regarding the bread produced, statistically significant year-related differences were observed for the parameters loaf volume during the first year (on average 298.75 cm3) and then increased in the second year (on average 417.33 cm3). Weight range 136.85 g to 145.18 g and moisture range 32.50 g/100 g to 39.51 g/100 g. Hardness range 8.65 N to 12.75 N and porosity (range 5.00 to 8.00) were closely related to the type of treatment. Finally, the color of flour and bread appeared to be not statistically significantly affected by treatment type. From a perspective of environmental and economic sustainability, the use of plant extracts with a bio-herbicidal function could replace traditional chemical herbicides.

Rational Design of Facile and Low‐Cost Construction of Carbon‐Based Dye‐Sensitized Solar Cells using Garcinia Mangostana L. as a Photosensitizer

Research on solar cell devices is not only always related to how to obtain high power conversion efficiency (PCE), but also other factors need to be fully considered, such as the cost and their ecofriendliness. In this present research, a low‐cost and ecofriendly dye‐sensitized solar cell (DSSC) is being developed using a graphite‐based nanocomposite and an unusual plant extract (Garcinia mangostana L.) as the dye photosensitizer. A rational design on how to use graphite as a photoanode and counter electrode (CE) in DSSC is carried out by combining the graphite with ZnO and multiwalled carbon nanotube (MWCNT), respectively. It is observed that graphite acts as a matrix for ZnO and MWCNT to make these nanocomposites have very appropriate optoelectronics and catalytic properties compared to the control sample. Therefore, they could serve as an excellent photoanode and CE, respectively. It is also expected that the anthocyanin absorption in Garcinia mangostana L. dye which is found in the visible light range can efficiently absorb photons, thus supporting the enhancement of photovoltaic performance of the DSSC. The generated voltage (V) and current (I) from light irradiation using commercial lamps are higher compared to halogen lamps, indicating that the obtained DSSC has potential as indoor powered devices. It is believed that our study can shed light on the development of DSSC using low‐cost material graphite as the main component for the realization of low‐cost DSSC devices.

Antibacterial and antifungal activities of Platycladus orientalis leaf extract-mediated Fe2O3 and Ce-doped Fe2O3 nanoparticles

Green synthesis of nanomaterials harnesses naturally occurring materials, including plant extracts, to offer environmentally friendly alternatives to conventional biomedicine, agriculture, and other field applications. This study explores the green route to Fe2O3 and cerium-doped Fe2O3 (Ce-doped Fe2O3) nanoparticles synthesized for the first time using the leaf extract of Platycladus orientalis. The synthesized nanoparticles were characterized for their structural, morphological, chemical, and optical properties. The hematite phase of Fe2O3 nanoparticles with spherical morphology was obtained. The introduction of Ce as a dopant into Fe2O3 increased the lattice strain of Ce-doped Fe2O3 nanoparticles (0.51%) compared to pristine Fe2O3 (0.46%) even though the size of both nanomaterials was similar. Compared to pristine Fe2O3 nanoparticles, Ce-doped Fe2O3 nanoparticles also demonstrated enhanced antimicrobial and antifungal activities against Escherichia coli, Enterococcus faecalis, Listeria monocytogenes, Penicillium chrysogenum, Aspergillus niger, and Mucor mucedo. The green-synthesized Ce-doped Fe2O3 nanoparticles possess potential for application in biomedical and environmental fields based on their relevance to human health and food safety, diversity in microbial characteristics, and potential for resistance to conventional treatments.

Traditional Medicinal Ranunculaceae Species from Romania and Their In Vitro Antioxidant, Antiproliferative, and Antiparasitic Potential

Several Ranunculaceae species are used in folk medicine to eliminate pathologies associated with oxidative stress as well as parasitic infections; however, a number of studies confirming their pharmacological properties is limited. In this study, 19 ethanolic extracts obtained from 16 Ranunculaceae species were assayed for in vitro antioxidant, antiproliferative, and antiparasitic potential. The maximum antioxidant potential in both oxygen radical absorbance capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays was observed for Aconitum toxicum extract [half-maximal inhibitory concentration (IC50) 18.7 and 92.6 μg/mL]. Likewise, Anemone transsilvanica extract exerted the most promising antiproliferative activity against Caco-2 (IC50 46.9 μg/mL) and HT29 (IC50 70.2 μg/mL) cell lines in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Additionally, a dual antioxidant and cytotoxicity effect was demonstrated for Aconitum moldavicum and Caltha palustris extracts. Whilst the efficacy of extracts was modest against Trypanosoma brucei (IC50 ranging from 88.8 to 269.3 µg/mL), several extracts exhibited high potency against Leishmania infantum promastigotes (Aconitum vulparia IC50 18.8 µg/mL). We also tested them against the clinically relevant intracellular stage and found extract of A. vulparia to be the most effective (IC50 29.0 ± 1.1 µg/mL). All tested extracts showed no or low toxicity against FHs 74Int normal cell line (IC50 ranging from 152.9 to >512 µg/mL). In conclusion, we suggest the above-mentioned plant extracts as potential candidates for development of novel plant-based antioxidant and/or antiproliferative and/or antileishmanial compounds.

Green synthesis of silver, starch, and zinc oxide mediated nanoparticles with probiotics and plant extracts, their characterization and anti-bacterial activity

Nanotechnology has various applications in all branches of science, including engineering, medicine, pharmacy, and other related fields. Conventional techniques, such as the chemical reduction approach, which produces nanoparticles (NPs) using various hazardous chemicals, offer several health risks due to their toxicity and raise serious environmental concerns. In contrast, other techniques are expensive and need a lot of energy. More than 70 % of pathogenic bacterial strains have developed resistance to at least one class of antibiotics, leading to an increase in life-threatening bacterial infections that pose a significant health risk. However, the creation of NPs by biogenic synthesis is risk-free for the environment and clean enough for biological use. This study was aimed at synthesis of novel Moringa oleifera mediated starch capped silver-zinc NPs and green synthesis of ZnO nanoparticles from Aloe vera, papaya, and Lactobacillus plantarum. Antimicrobial activity of both NPs was tested against Gram-negative antibiotic-resistant bacteria Pseudomonas aeruginosa, Gram-positive bacteria Staphylococcus aureus (ATCC 6538), and two foodborne pathogens Listeria monocytogenes and Campylobacter jejuni. Ultraviolet–visible spectroscopy, Fourier Transform Infrared Spectroscopy, and Scanning Electron Microscopy were used for characterization. Majority of the research studies stress the flexibility, repeatability, and desirable features of the metals, polymers, and plant components employed in the production of biomedical nanoparticles. Such an intuitive approach provides several advantages, particularly a reasonable total expense, compliance with healthcare and pharmaceutical implementations, and the ability to produce massive volumes for industrial use. The novelty of the presented work lies in the unusual combination of silver, starch, and zinc oxide nanoparticles using Moringa oleifera, which is an eco-friendly alternative to chemical-based methods. This research exhibits the formation of well-defined nanoparticles with strong antibacterial activity against a wide range of pathogens, giving us insights into their potential applications in various biomedical fields.

Preparation and Characterization of Poly(lactic acid)-Based Poly(ethylene glycol) and Daphne Essential Oil-Loaded Smart Nanofibers for Thermal Protection.

Phase change material (PCM) stores latent heat energy, and poly(ethylene glycol) (Mw: 4000) (PEG 4000) is also a solid-liquid PCM. PEG and poly(lactic acid) (PLA) polymers are biodegradable. Essential oils are known as plant extracts with antimicrobial properties. In this study, daphne essential oil (DEO) obtained by the distillation method and PLA/PEG/DEO composite nanofibers were prepared by the electrospinning method with PLA, PEG 4000, and daphne (Laurus nobilis L.) essential oil in certain ratios (100/100/20, 100/120/20, and 100/150/20). DEO showed an antibacterial effect against Staphylococcus aureus and Escherichia coli bacteria. Thermal behaviors of the nanofibers were characterized by differential scanning calorimetry and thermogravimetric analysis. Morphological features were observed by scanning electronic microscopy (SEM), crystal behavior by X-ray diffraction analysis, and molecular structures were examined by Fourier transform infrared spectroscopy. Essential oil composition was determined by GC-MS. The thermal decomposition temperatures of the nanofibers were found between 250 and 276 °C, and the latent heat storage energies of nanofibers were 69.06, 86.76, and 96.39 J g-1 at temperatures 59.0 and 54.37 °C. High PCM added fiber was observed as 182 nm diameter with 3.264 μm diameter spheres. The produced nanofiber matrix has the potential to be used in applications such as medicine, textile, and hot food logistics.

Ethanolic duea ching extract in combination with microbial transglutaminase: Strengthening effect on mince gel and cross-linking of myofibrillar proteins from Indian mackerel

Surimi or fish mince gel are popular seafood products worldwide. Lean fish are commonly used as raw material, while dark fleshed fish, especially Indian mackerel, has high fat content and dark color associated with poor gel quality. To improve gel strength, plant extract, particularly from duea ching (Ficus botryocarpa Miq.), rich in polyphenols and calcium could be used as natural protein cross-linker to strengthen gel network. Effect of ethanolic duea ching extract (EDCE) at varying levels in the absence and presence of microbial transglutaminase (MTGase) on gel property of Indian mackerel mince gel prepared from fillets pre-washed for different times (1 and 3 times) was studied. Heat-induced aggregation of myofibrillar protein (MP) from Indian mackerel fillets under different conditions was also investigated. Mince gel prepared from fillets pre-washed for 3 times added with 0.10 % EDCE and 0.5 unit/g of MTGase (3-PWM-MT-0.10) showed the highest breaking force, deformation and textural properties (P < 0.05). Lower autolysis of mince gel was obtained when EDCE, MTGase or in combination were added. However, addition of ECHE decreased whiteness of mince gel, particularly at higher concentrations (P < 0.05). Electrophoretic images revealed the disappearance of myosin heavy chain when MTGase or EDCE in conjunction with MTGase was incorporated. Addition of 0.10 % EDCE, MTGase or both could induce aggregation and enhance setting phenomenon of MP solution as indicated by higher absorbance at 660 nm and storage modulus (G′), respectively. Based on microstructure study, 3-PWM-MT-0.10 had more compact, finer and interconnected structure than other gels. Therefore, the use of EDCE and MTGase at the appropriate levels could be used for improving quality of mince gel from pre-washed Indian mackerel fillets.

Plant-Based Ingredients Utilized as Fat Replacers and Natural Antimicrobial Agents in Beef Burgers.

The present study aimed to find solutions based on the use of plant-based ingredients that would improve the nutritional quality of meat products as well as ensure sensory and microbiological quality. Two fat replacers, lemon albedo (Citrus lemon) and carob seed gum (Ceratonia siliqua), were investigated by chemical analysis and panel testing to evaluate their effect on the nutritional and sensory quality of beef burgers. The antimicrobial activity of two plant extracts, from nettle (Urtica dioica) leaves and medlar (Eriobotrya japonica) seeds, was studied, evaluating the intensity of inhibitory action and the minimum inhibitory concentration against Pseudomonas spp. and Listeria innocua strains by plate test. In addition, the antioxidant activity of both extracts was evaluated. Based on the results, lemon albedo and medlar seed extracts were validated in a food model (beef burger) by a storage test and a challenge test. The storage test results highlight that medlar seed extract prevents the formation of thiobarbituric acid reactive substances (TBARSs) and ensures microbiological quality, inhibiting Enterobacteriaceae and Pseudomonas spp. Anti-Listeria efficacy was confirmed in situ by challenge test results. In conclusion, although fat replacers ensure nutritional and sensory quality, they do not satisfy microbiological quality. This study clearly demonstrates that the safety of low-fat burgers can only be achieved through the combination of appropriate fat replacers with well-selected natural antimicrobial extracts.

Eco-Friendly Green Synthesis of Chromium Oxide Nanoparticles and Their Characterization and Application

In recent years the nanoparticle design synthesis methods conducted by plants replaced the traditional chemical procedures of nanoparticles preparations. This study demonstrates the environmental kindly preparations of chromium oxide nano particles (Cr2O3-NPs) using leaf extract of Mentha pulegium plant. The obtained Cr2O3-NPs was characterized by Ultra Violet /Visible spectroscopy, Fourier Transform Infra-Red (FTIR), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray spectrophotometer (EDX) and X-ray diffraction studying. The presence of the Sharp peak at 462 nm in the Ultraviolet/Visible spectrum and the color changed by surface plasma resonance explains that Cr2O3-NPs is formed. The presence of oxygen and chromium in Cr2O3-nanoparticles is reported by EDX spectrum, which was found to consist of 32.57% Cr and 44.41% O, confirming the high purity of the Cr2O3-NPs powder. Since Scan Electron Microscope studying reported an irregular round morphology shape. In addition, X-ray diffraction studying suggested their crystalline feature by the characteristic peaks at 2θ= 24.3°, 33.5°, 36.2°, 41.5°, 50.3°, 54.8°, 63.4°, and 65.2°, respectively. The average crystallite size for Cr2O3-NPs was found to be 58 nm. The photo catalytic activity of green synthesized chromium oxide-NPs was also analyzed.

Methanol Extract of Thottea siliquosa (Lam.) Ding Hou Leaves Inhibits Carrageenan- and Formalin-Induced Paw Edema in Mice

Inflammation is a physiological condition that when unattended causes serious health concerns over the long term. Several phytocompounds have emerged as promising sources of anti-inflammatory agents. Thottea siliquosa is a traditional medicine for inflammatory and toxicity insults; however, this has not been scientifically confirmed. The purpose of this study is to evaluate the anti-inflammatory properties of T. siliquosa methanol leaf extract in a mouse model. This study investigates the anti-inflammatory activities of a plant extract obtained from leaves of T. siliquosa (TSE) with a focus on carrageenan- and formalin-induced paw oedema in mice. The extract’s efficacy was assessed using well-established inflammation models, and the results showed a considerable reduction in paw edema in both cases. In the case of carrageenan model TSE at 50 mg/kg showed a 53.0 ± 2.5% reduction in edema, while those treated with TSM at 100 mg/kg exhibited a 60.0 ± 1.8% reduction (p &lt; 0.01). In the case of a formalin model when a higher dose of TSE (100 mg/kg) was given, paw thickness decreased by 47.04 ± 1.9% on the fifth day and by 64.72 ± 2.2% on the tenth day. LC-MS analysis reported the presence of gallic acid, quinic acid, quercetin, clitorin, myricitrin, retronecine, batatasin II, gingerol, and coumaric acid in the extract. Overall, this study confirms that T. siliquosa extract exerts anti-inflammatory effects in animals and is possibly mediated through the combined effects of these phytochemicals.

Silver loaded functionalized calix [4]arene nanofibers and evaluation in burn wound healing

Burn treatments with gene therapy and healing factors requires highly cost techniques. Instead, topical formulations including plant extract or pharmaceuticals are offered for wound-healing effect. However, these have low water solubility and cannot uptake into cells sufficiently. To overcome disadvantages, new effective treatment approaches should have been accessible. This study aimed to develop nanofiber-based wound coverings using four different Ag(I)-calixarene derivatives. Their nanofibers were produced by the electrospin method, and evaluated in vivo in rats with a second-degree burn model. Biochemical analysis has been maintained to exmine the effects of formulations on such as growth factors and some cytokines that are effective in wound healing process. In addition, histopathological analyzes has been performed to evaluate the presence and density of granulation tissue, inflammation, epithelial thickness and fibrosis. All data were compared with a commercial topical, silver sulfadiazine, whose usage is limited due to various disadvantages in the treatment processes of burn wounds. In this study, four different calixarene derivatives were synthesized their nanofibers were produced by the electrospin method, and their effectiveness was evaluated in vivo in rats with a second-degree burn model. Then, various biochemical and histopathological analyses were done to elucidate the possible wound-healing mechanism. When considering both pathological and biochemical data, the nanofibers of CLX-SAMD-Ag (5,11,17,23‐tetra‐tert‐butyl‐25,27‐bis(Sulfadiazine)‐26,28‐propylcalix (Xie and Unser, 2013) [4]arene - Ag) and CLX-MORF- Ag (5,11,17,23‐tetra‐tert‐butyl‐25,27‐bis(3-Morpholinopropil)‐26,28‐dihydroxycalix (Xie and Unser, 2013) [4]arene-Ag) were found to be more effective than silver sulfadiazine -silver content application, also known as traditional burn treatment for wound healing.

Biosynthesis of antimicrobial nanoparticle from Swertia spp. (Chirayita) against bacterial pathogens of Poultry- A way forward to Green Nanotechnology and Nano-medicines.

The increasing prevalence of multidrug-resistant microorganisms in poultry has led to a rise in bacterial infections, causing significant economic loss. Green nanotechnology, such as silver nanoparticles (AgNPs), has the potential to address this issue by providing potent antifungal, antiviral, and antibacterial properties. This study explored the combined potential of AgNPs and the local herb Swertia chirayita against established poultry pathogens, employing a non-factorial Central Composite Design (CCD) to evaluate the factors affecting the production of nanoparticles induced by silver nitrate from the selected herb. The optimal values for temperature, wavelength, silver nitrate concentration, incubation duration, and pH were found to produce the highest nanoparticles. The functional groups in Swertia chirayita stimulated nanoparticles were confirmed using FTIR spectroscopy, and the stability of ScNPs was elucidated using zeta potential. The crystalline structure of ScNPs was confirmed using diffraction intensity patterns. Silver nanoparticles demonstrated antibacterial activity against Salmonella spp. and Escherichia coli (E.coli), both known as significant poultry pathogens, using the agar well diffusion method, with inhibition zones of 25.0 mm and 35.0 mm, respectively.This study explored the green manufacturing of silver nanoparticles by using plants and microorganisms, focusing on their antibacterial properties. The exact mechanism of synthesis and action in AgNPs is still poorly understood. Researchers should prioritize the use of accessible, easy-to-extract plants or bacteria, especially non-pathogenic and fast-growing microorganisms for safe handling. Analyzing biomolecules in plant extract, microbial biomass, or culture supernatants, including probiotic bacteria, is crucial for creating and stabilizing AgNPs, which could be effective synthetic agents. It is crucial to optimize conditions for rapid, stable, and large-scale synthesis. Based on this research, Sc-NPs may be proposed as nanomedicine for treating infections in poultry caused by E. coli and Salmonella spp.

Use of Metallic Nanoparticles Synthesized from Plant Extracts in Wound Healing—A Review

Use of Metallic Nanoparticles Synthesized from Plant Extracts in Wound Healing—A Review