Neem Extract Research Articles

Copaiba oil and Neem extract can be a potential alternative for the behavioral control of Sitophilus zeamais.

Insects' ethology is an important factor when it is desired to carry out pest management. This knowledge makes it possible to manipulate behavioral activities, repel, or attract insects according to needs and interests. The maize weevil Sitophilus zeamais (Mots., 1855) (Coleoptera: Curculionidae), one of the main stored grain pests, has been the target of studies of behavioral changes studies through natural substances due to its resistance to different insecticidal classes. Thus, this study aimed to evaluate the effect of sublethal concentrations of neem extract and copaiba oil on the locomotor behavior of S. zeamais. The behavioral characteristic considered were walking activity, the frequency of contact of insects with the treated grain mass, and the time spent for this behavior. The walking activity of the S. zeamais increased with exposure to Neem extract and Copaiba oil. In general, the Neem extract and Copaiba oil-induced more contact with grain mass than the control, suggesting an attractive effect on the insect, however more significant for the Neem oil. The insect's behavior was altered, presenting a specific path due to Copaiba oil and Neem extract stimuli. These results indicate that Copaiba oil and Neem extract can be a potential alternative for controlling S. zeamais on stored products since changes in this pests' behavior can reduce qualitative and quantitative grain damage. Thus, the development of products based on Copaiba oil and Neem extract may be helpful for storage pest management.

Immunological studies on the effects of toltrazuril and neem extract in broiler chickens suffering from coccidiosis.

The prevalence of avian coccidiosis in the poultry industry has grown, resulting in substantial financial losses from high mortality, stunted growth, reduced productivity, and expensive medical expenses. The purpose of the current study was to assess the immunological effects of neem leaf extract and toltrazuril on broilers that had contracted coccidiosis. In this investigation, 100 one-day-old Cobb broiler chicks without sexes were employed. The chicks were divided into five equal groups, with 20 birds in each. On the 14th day of life, the birds in groups 2, 3, 4, and 5 received an oral inoculation with 1 × 105 sporulated oocysts of Eimeria tenella (E. tenella) (field isolate). The first group (Gp), which consists of 20 healthy broilers, served as a negative control. Gp (2) contains experimentally infected broilers and nontreated (served as a positive control). Gp (3) contains experimentally infected broilers treated with toltrazuril (1 ml/l drinking water) for two consecutive days. Gp (4) contains experimentally infected broilers treated with neem leaf extract 4% (50 ml/l drinking water) for 5 successive days, and Gp (5) contains experimentally infected broilers treated with toltrazuril (1 ml/l drinking water) and a half dose of neem leaves extract 4% (25 ml/l drinking water) for 5 successive days. For the purpose of estimating body weight growth and feed conversion ratio, each broiler was weighed separately at the start of the trial and again on the 1st and 10th day after treatment. In addition to obtaining intestinal samples for immunohistochemistry, blood samples were also obtained for immunological examination. As compared to the negative control group, the experimentally infested broilers with E. tenella showed significant decreases in serum nitric oxide, lysosome, phagocytic percent, and phagocytic index, along with significant increases in white blood cells (WBCs), lymphocyte, heterophilis, eosinophilis, basophilis, monocyte, serum total protein, γ globulin, fibrinogen, and haptoglobin. When compared to the control positive group, experimentally infested broilers treated with either neem or toltrazuril alone or in combination demonstrated significant increases in serum total protein, nitric oxide, lysozyme, phagocytic percent, and phagocytic index, but significant decreases in WBCs, lymphocytes, heterophile, eosinophile, basophile, and monocyte. The intestinal peroxidase stain of broilers infected with E. tenella exhibited a significant positive expression for CD4, but the infected broilers treated with toltrazuril and half a dosage of neem displayed a negative expression for CD4, identical to the negative control. The broiler chickens infested with E. tenella may have a variety of negative impacts on their immune systems and immunohistopathological findings. Nonetheless, toltrazuril and neem extract, either separately or in combination, function as anticoccidial medications that may enhance the broiler chicks' immune state.

Antibacterial Activities of Silver Nanoparticles Synthesized from plants and Fungi

The present study aimed to synthesize silver nanoparticles (AgNps) having antibacterial properties. Two fungal strains, viz Aspergillus niger and A. terreus were procured from NCIM, Pune. And were used to synthesize silver nanoparticles by reduction and precipitation of AgNO3 . The aqueous plant extract of Neem (Azadiracta indica) leaves was prepared for the synthesis of AgNps. All the AgNps derived from fungi and plant sources were evaluated for antibacterial properties against known laboratory pathogens. The obtained nanoparticles were characterized using a UV-visible spectrophotometer and SEM. Silver nanoparticles were produced significantly from A. niger at 400nm and A. Terreus at 400 nm. The aqueous extract of the neem plant showed maximum absorption at 500 nm. Also, the extract nanoparticles showed the highest antibacterial activity against E. coli, Proteus vulgaris, Pseudomonas spp, Salmonella paratyphi-A and S. aureus.

Incorporating neem leaf extract for enhanced sock functionalities: an eco-friendly approach

Incorporating neem leaf extract for enhanced sock functionalities: an eco-friendly approach

Hematological effects of Azadirachta indica (Neem) extract on New Zealand white male rabbits: A comprehensive study

Hematological effects of Azadirachta indica (Neem) extract on New Zealand white male rabbits: A comprehensive study

An investigation of biopolymers in corrosion resistance under industrial exposure condition

India, a developed country with a bounty of resources, has produced a large number of biopolymers that were employed in historical construction. If these biopolymers are proven through research to have positive aspects, it would eventually result in the development of environmentally friendly, cost-effective, and long-lastingstructures. Although many of the efficient inhibitors, like chromate and arsenate, are exceedingly poisonous, the price of inorganic inhibitors is generally inexpensive. As neem and turmeric extracts are readily available and may be obtained year-round in concrete, they should be further investigated as corrosion inhibitors in various ratios and combination. This experimental investigation can be used to provide a corrosion inhibitor that is affordable, non-toxic, eco-friendly, and user-friendly.The application of biopolymer in concrete has been supported by the results of the feasibility study, which additionally showed promising result for aexcellent corrosion inhibitor from the phytochemical analysis and results for compressive strength.

Antibacterial Efficacy of Plant Aqueous Extracts Against Escherichia coli O157:H7 Bacteria in Grenada, West Indies

Antimicrobial resistance has developed as a major health problem worldwide, endangering the efficacy of antibiotics and imposing a severe burden of resistant infections. The progressive increase in the prevalence of multiple resistant bacterial strains paves the way for the selection of natural plants with antibacterial properties as an alternative substitution for the treatment of bacterial infections. Hence, this study was conducted to determine the antibacterial efficacy of neem (Azadirachta indica), moringa (Moringa oleifera), and curry leaf (Murraya koenigii) against Escherichia coli (E coli) O157:H7 isolated from a fecal sample of a goat from Grenada. This is the first medicinal plant-based research study in Grenada on this important zoonotic pathogen. Aqueous extracts of medicinal plants were evaluated for antibacterial efficacy in vitro, using the agar well diffusion method, and subsequently compared with the antibacterial drug gentamicin. The resulting data demonstrated that aqueous plant extracts of neem leaves, moringa leaves, and curry leaves exhibited in vitro antibacterial efficacy comparable to that of gentamicin against E coli O157:H7 goat isolate. Hence, these plants could be used as an effective substitute for antibacterial agents in prophylactic or therapeutic control of bacterial infections in Grenada.

Renovation of spoiled sorghum by incorporation of neem extract for biodegradable films

Renovation of spoiled sorghum by incorporation of neem extract for biodegradable films

Farmer perception, knowledge and management of the scale insect pest complex infesting crops and trees in Coastal Kenya

Scale insects and mealybugs (Hemiptera: Sternorrhyncha: Coccomorpha) are serious plant sap-sucking pests affecting a wide range of cultivated crops and tree species. The insects are highly diverse and can have devastating effects on yields due to lack of farmer awareness and inappropriate management practices. Recent alien mealybug invasions in Kenya include among others, papaya mealybug (Paracoccus marginatus), a highly invasive pest that is spreading quickly. Farmer experience with diverse crop pests can support development of a successful pest management strategy and contribute to reduced impacts on both crop yields and agro-biodiversity. This socio-economic survey was carried out in three coastal counties of Kenya (Mombasa, Kilifi and Kwale) to establish the impact of scale insect and mealybug pests on farmer livelihoods and to document the perception, knowledge and management practices used by smallholder farmers. Data from oral interviews using ODK were administered to respondents and analysed using Excel and GENSTAT. It emerged that 26% of the respondents were familiar with scale insects and 51% with mealybugs, respectively. Of these, 78.13 % and 94% acknowledged having encountered scale insects and mealybugs, respectively, on their farms. The farmers confirmed that scale insect pests affected a high diversity of crops and trees. About 56% of the respondents used pesticides to control the pests while 25% did not apply any management strategy. The remaining 19% practiced cultural control methods such as field sanitation and intercropping with less susceptible crops. Pesticides were reported to be moderately effective (41-70%) at controlling scale insects and mealybugs. Other management options reported by farmers included farm hygiene, the use of high-pressure water jets, and applications of ash and bio-pesticides such as home-made neem extract. Based on the findings, it is recommended that capacity building for farmers and input providers be undertaken to enhance the knowledge of scale insect and mealybug pest biology, symptoms of crop and tree attack, host ranges and management practices. Additonally, training on the use of low-risk pest control products and innovative control methods should be undertaken to reduce the impact of the pest on crop production and agro-biodiversity. Keywords: Farmer knowledge, management, mealybugs, scale insects and socio-economic survey.

تأثير مستخلصي الثوم والنيم في مكافحة مسبب مرض نسيج العنكبوت والعفن الاخضر في محصول الفطر الابيض Agaricus bisporus

The study was conducted to control cobweb disease caused by Cladobotryum dendroides and green mold disease caused by Trichoderma aggressivum, which infect the white fungus Agaricus bisporus crop. The laboratory results on the pathogenicity of the two pathogenic fungi showed their ability to cause infection in the white fungus and the emergence of symptoms. The addition of garlic and neem extracts, separately, at three concentrations (0.25, 0.5, and 1%) to the media, potato dextrose agar (PDA), led to a significant decrease in the percentage of inhibition of pathogenic fungi C. dendroides and T. aggressivum. The highest inhibition rates obtained when using garlic extract at a concentration of 1% against the two pathogenic fungi amounted to 62.93 and 74.97%, respectively, while the name extract recorded the highest inhibition rate at the same concentration, reaching 43.47 and 32.37%, respectively. Also, when testing the extracts in the traditional cultivation of white fungus, it was noted that the garlic extract at a concentration of 1% was significantly superior in controlling infection, and no symptoms appeared when using it, the use of plant extracts at different concentrations did not record a significant difference in the period required for the emergence of fungal pins compared to the control treatment without a pathogen, the highest amount of white fungus production was 1000 g/bag when using garlic extract at a concentration of 0.5% in the presence of the pathogen C. dendroides, while the highest amount of production was 1355.0 g/bag when using neem extract at a concentration of 0.25% when infected with the pathogen T. aggressivum.

Botanical Leaf Extracts and Mulching Materials in Organic Eggplant (Solanum Melongena L.) Off Season Production

The extensive use of pesticides and the risk they pose to human and the environment requires safe alternatives in crop production hence the study on the efficacy of botanical leaf extracts and mulching materials in organic eggplant production. This study determined the best level combination of botanical leaf extracts that has repellent and knockdown effects on major eggplant insect pest, best level combination of botanical leaf extracts that produces the highest marketable yield; best mulching materials; best level combination of botanical leaf extracts and mulching; correlation of infestation to yield; profitability of eggplant production using botanical leaf extracts and mulching materials combined; and evaluate the effects of botanical leaf extracts and mulching materials on soil chemical properties. A Bioassay test on the botanical leaf extracts was conducted prior to field experiment. Mass rearing the test insects was done to ensure uniform number of insect in eggplant per treatment. A Split- plot in Factorial Design was used in the study with three blocks. The main plot were the leaf extracts of Neem, Madre de Cacao+ Guava at 1:1:1 ratio were: P0 – No Application, P1 –250 ml leaf extract/16 li water, P2 –500 ml leaf extract /16 li water, P3 –750 ml leaf extract /16 li water, P4 – 40 ml Commercial insecticide/16 li water. The sub-plot was: M1 – Black Plastic mulch, M2 – Carbonize Rice Hulls, and M3 – Saw Dust. Results of the bioassay showed highly significant knockdown effect in the application of 500-750ml leaf extract/16 li water on fruit and shoot borer, lady spotted beetle and white flies comparable to application of commercial insecticide/16 li water. Significant repellent effects on Fruit Shoot Borer and Lady spotted beetles but no repellent effects on white flies for an application of 250ml botanical leaf extracts/16li water. Results under field condition showed that plants applied with 750ml botanical leaf extracts/16 li water is more effective than commercial insecticide/16 li water in reducing damaged leaves during the early vegetative stage of development (2nd WAT) and at reproductive stage of development (10th WAT). Highly significant difference was obtained in terms in weight of non-marketable fruit. No significant interaction was registered between botanical leaf extracts and mulching materials. Regression correlation analysis revealed that there was a direct correlation between the numbers of damaged leaves to yield. Combined application of botanical leaf extracts at 750ml/16 li water + carbonized rice hulls obtained Php 797,635.15 net for organic off-season eggplant production. Soil pH, OM, P, and K were increased with application of sawdust, carbonized rice hulls and plastic mulch.

Effectiveness of GRAS substances for managing leaf diseases in brinjal (<em>Solanum melongena</em> L)

Leaf diseases in brinjal (Solanum melongena L.) are common and drastically lower its yield. As synthetic fungicides are hazardous to the environment and human health, non-fungicidal approaches to foliar disease control are being adopted progressively. The current investigation assessed the effectiveness of Generally Recognized as Safe (GRAS) substances in the management of leaf diseases in brinjal. The efficacy of the selected GRAS substances, including 400 μM of salicylic acid, 0.1% of sodium bicarbonate, and botanical extracts such as 200 ppm of garlic clove extract, 200 ppm of neem extract and 200 ppm of eucalyptus extract, were assessed. Six treatments and four replicates were employed in a completely randomized design for this present study. The natural occurrence of leaf diseases was taken into account in the experiment. Disease incidence (%), disease severity (%), number of leaves per plant, plant height (cm) and number of branches per plant were recorded seven weeks after transplanting. Analysis of variance was used to assess the significance of treatment effects. The findings confirmed that the exogenous spraying of salicylic acid and garlic clove extract significantly (P<0.05) decreased the disease severity and incidence, with statistically equivalent efficiency for both substances. Moreover, number of branches per brinjal plant (3.3), plant height (51.4 cm) and number of leaves per brinjal plant (31.5) were significantly (P<0.05) enhanced by salicylic acid compared to control. Hence, spraying garlic clove extract and salicylic acid may therefore be an environmentally potential means of managing brinjal leaf diseases during the vegetative growth stage.

FORMULASI DAN EVALUASI SEDIAAN FACIAL WASH EKSTRAK MIMBA (Azadirachta indica A.Juss)

Neem extract is known to have antibacterial activity against acne-causing bacteria. Neem extract is made into facial wash preparations to develop herbal plants into more modern preparations. This study aims to determine the optimal formulation from the results of the physical evaluation and hedonity test and then find out that there are differences from the results of the physical evaluation of neem extract (Azadirachta indica A.Juss) facial wash preparation. The research method used was a pre-experimental laboratory one-shot case study. Physical evaluation tests included organoleptic tests, homogeneity, pH, viscosity, free alkali, foam height/foam stability, free fatty acids and hedonity (favorability test). The results of the research on the organoleptic test obtained optimal results in formula 3 and the results of the homogeneity test obtained a homogeneous preparation. Optimal pH test results in formula 3. Optimal viscosity test results in formula 3. Free alkali test results from the three formulations did not contain free alkali. Optimal foam height/foam stability test results in formula 3. Free fatty acid test results do not meet SNI facial wash standards. The results of the hedonity test showed that formulation 3 was preferred by respondents. The optimal facial wash preparation is formulation 3.

Investigation of Bio Fungicidal Properties of Crude Extracts of Neem Leaf against Blast Disease of Finger Millet (Eleusine coracana)

The efficacy of neem leaf extract against finger millet blast pathogen Magnaporthe grisea was assessed invitro. The neem leaf extract significantly inhibits the pathogen mycelial growth and melanin biosynthesis. Neem leaf extract shows 84.33 % inhibition of the pathogen over control. Volatile test infer that no volatile compounds were found to be involved in growth inhibition and melanin production inhibition. In field experiments foliar spray with neem leaf extract 5%, 10% and 15% along with chemical Tricyclazole 0.1% and Picoxystrobin 6.78% + Tricyclazole 0.1 % was done. Data recorded on leaf blast PDI at 15, 25 and 40 days after transplanting revealed that lower leaf blast PDI of 17.72, 16.45 and 15.45 respectively was recorded in neem leaf extract (T1,T2,T3) which is comparatively similar with chemical treatment 1 and 2 PDI 13.00 and 10.90. In neem leaf extract treated field a significant yield increase was also observed.

Development and Characterization of Polyherbal Anti-acne face gel using Liquorice and Palash extract

Anti-acne herbal formulations are used to treat acne vulgaris and also have the added benefit of not having any side effects, unlike synthetic medications. Acne is a bacterial-induced inflammatory skin disease caused by blockages in the pilosebaceous and inflammation. Antibiotics, both topical and systemic, are always used to treat acne, however antibiotic resistance can impair the success rate of acne treatment. The creation of effective therapeutic medicines relies heavily on medicinal plants. So, natural medicines are more acceptable because they are believed to be safer and have fewer adverse effects than manufactured ones. In the global market, herbal formulations are in high demand. The goal of this study is to produce and characterise herbal anti-acne face gel that contains neem (Azdirachta indica) extract, liquorice root (Glycyrrhiza glabra) extract, orange peel (Citrus aurantium var. sinensis) extract, and palash (Butea monosperma) flower extract. Although there are a variety of topical herbal formulations for acne on the market, we recommend creating a polyherbal formulation using a variety of herbal and synthetic ingredients. The plant has been found to have antimicrobial and anti-inflammatory properties in the literature. The prepared extract was screened to identify the presence or absence of various active principles like phenolic compounds, carbohydrates, flavanoids, glycosides, saponins, alkaloids, protein and amino acid. Polymers in various proportions were used to create several formulations ranging from F1 to F8. Colour, appearance, consistency, washability, pH, and spreadability and stability of prepared formulations (F1 to F8) were all examined.

Optimal growth conditions for avocado (Persea americana Mill.) seedlings using biofertilizer-based approach with biosurfactant diethanolamide palm olein and neem extract

Avocado is a much-loved fruit with high nutritional content. However, its current production has declined due to excessive and continuous use of chemical fertilizers. Biofertilizers can be applied to reduce the reliance on chemical fertilizers, promoting soil health and fertility by providing essential macro and micronutrients for better plant growth. This study aimed to determine the optimal dosage and method for the combined application of biofertilizer, biosurfactant diethanolamide (DEA) palm olein, and neem extract on avocado seedlings. The experimental design used was a factorial randomized complete block design with two factors. The first factor was the biofertilizer dosage (B) and the second factor was the application method (M). Results indicate that a 10 mL/L biofertilizer dosage, 5% DEA, and 35% neem extract show the best effects on plant height, leaf chlorophyll levels, stem diameter, and the number of avocado leaves. The application method of applying to the planting medium yields the best results for plant height, leaf count, and leaf chlorophyll levels. There was an interaction between treatments regarding plant height at 10 weeks after transplanting (WAT) and leaf count at 8 WAT. Biofertilizer application by pouring is recommended as an alternative to inorganic fertilizers for promoting healthy avocado seedling growth.

Exploring the Therapeutic Potential of Azadirachta indica (Neem): Recent Advances and Applications

This review explains the therapeutic prospective of Azadirachta indica (Neem), a traditional medicinal tree in India, Southeast Asia, and Africa. Neem extracts have been found to have various therapeutic properties, including antimicrobial, antifungal, hepatoprotective, antiulcer, antifertility, and antinociceptive properties. Recent research has shown neem’s potential as an antiviral agent, cancer treatment, and a source of antibiotic compounds. Neem oil nano hydrogel shows significant antimicrobial activity against various pathogens. The plant also has the potential to mitigate heavy metal pollution and develop a model for predicting soil remediation using neem leaves. Neem’s inhibitory activity on papain-like protease of SARS-CoV-2 is also explored. Overall, neem’s therapeutic potential is significant and its potential in healthcare and environmental remediation is highlighted.

Evaluating the efficacy of different botanicals on prolonging shelf life and maintaining quality of mandarin (Citrus reticulate Blanco var. Banshkharka Local)

Mandarin (Citrus reticulate Blanco) has been cultivated in 56 districts of Nepal. Huge losses in mandarin were reported and use of safer alternatives to synthetic chemicals to solve post-harvest losses is a matter of great concern. An experiment was conducted at the Directorate of Agricultural Research, Lumle for two consecutive years 2019 and 2020. Mandarin having similar maturity indices were harvested and transported to DoAR, Lumle. The fruits were treated with five different botanicals (garlic extract @ 10 %, ginger extract @ 10 %, aloe vera extract @ 10 %, neem extract @ 10 %, and control) allocated in randomized complete block design with four replications. The post-harvest study was conducted for 3 weeks under laboratory conditions (7.9 ± 3° C and RH 74 ± 4%). Different parameters such as physiological loss in weight (PLW), decay loss, total soluble solid (TSS), titrable acidity (TA) and fruit weight to juice ratio were recorded. Fruit treated with ginger extract @ 10 % recorded the minimum physiological loss in weight (6.31% and 5.15%), minimum decay loss (16 % and 17%) in the year 2019 and 2020, respectively. Highest TSS: TA ratio (13.54 and 12.79) and fruit weight to juice ratio (0.33 and 0.35) was also observed in fruit treated with ginger extract @ 10 % in both years. In both years, results obtained in the fruits treated with ginger extract was followed by fruit treated with aloe vera extract and garlic extract. Control treatment gave the poor result as compared to ginger extract @ 10 %. Thus, this study concluded that the use of ginger is suitable for postharvest treatment of mandarin fruit to increase its shelf life and maintain its quality parameters.

Formulation of Herbal Topical Dermatological Dosage Form by Quality by Design Approach

Objective: The study developed an optimized dosage form by quality by design (QbD) approach containing Azadirachta indica (Neem) leaf extract and a combination of selected herbal oils viz. A. indica oil, Eucalyptus citriodora oil and Cymbopogon martini oil and assessed for its antifungal efficacy, stability and dermal safety. Methods: Ointment was prepared using the fusion method from an extract of neem leaves with a combination of herbal oils viz. A. indica, E. citriodora, C. martini, bees wax and soft-paraffin. Utilizing the Box-Behnken Design (BBD), the variables such as the percentage of soft paraffin, beeswax and melting temperature were optimized in relation to the output variables such as viscosity, spreadability, and finally the antifungal efficacy, which was further quantified. Wistar albino rats were used (n = 5/test, positive and negative control) to evaluate the acute dermal toxicity test of the formulated ointment. Stability studies were assessed at 25 ± 2℃/ 60 ± 5% RH and 40 ± 2℃/ 75 ± 5% RH. Results: Melting point and percentage of beeswax significantly affect viscosity and spreadability. Optimal viscosity was obtained at 5.4% beeswax and 89.6% soft-paraffin when melted at 58℃. No dermal toxicity was observed by the ointment when comparable to petroleum jelly, both differed significantly with negative control. The absence of new spots on chromatograms, a prominent zone on agar plates, and negligible changes in spreadability (p = 0.112) all suggested physical stability, chemical stability, and antifungal efficacy, respectively. Conclusion: Neem leaf extract and a blend of selected herbal oils; A. indica, E. citrodora, and C. martini, when combined to formulate ointment, proved reliable and stable dosage form with significant anti-fungal efficacy. The formulated ointment might be helpful tool for dermatophytes management.

Green Synthesis of Sulphur Nanoparticles Using Azadirachta indica (Neem Leaf) and Its Characterization

Nanoscience has found various applications in different biomedical fields. The synthesis of nanoparticles (NPs) has become a vast area of research due to its potential applications. These particles can be prepared by different chemical, physical and biological approaches. In recent years, green synthesis of NPs using plant extracts has gained much interest due to non-toxicity and very low cost of synthesis. The plant extracts act both as reducing or stabilizing agent as well as capping agent. Neem (Azadirachta indica A. Juss) is a well-known medicinal plant and has been studied for the biosynthesis of NPs. A. indica has various phytochemicals identified that can reduce the metal ions. The bio reduction of NPs from neem extract is an eco-friendly, low cost and green synthesis method. These NPs are reported to exhibit good antimicrobial, mainly antibacterial activity. NPs was characterized by Dynamic Light Scattering (DLS) for particle size, particle size distribution, poly- dispersity index, zeta potential, UV visible spectrophotometer and Fourier-Transform Infrared Spectroscopy (FT-IR) for functional characteristics. Dynamic light scattering measurements for particle size (nm) and Polydispersity Index showed that the particle size was in the range of 35-40 nm with Polydispersity Index of 0.280-0.290, it is the mid-range value and indicating that the particles were in disperse form in aqueous suspension. From the analysis, the zeta potential value was found to be (-5.77 mV), revealing the better stability of synthesized sulphur nanoparticles in aqueous suspension. The FT-IR spectrum showed characteristic absorption of wavenumber from 3384.73 cm-1 and 533.56 cm-1. The absorption spectra of UV-visible spectrophotometer in between the range of 200-800 nm.