Extraction Of Water-soluble Polysaccharides Research Articles

New freeze-thaw method for improved extraction of water-soluble non-starch polysaccharide from taro (Colocasia esculenta): Optimization and comprehensive characterization of physico-chemical and structural properties

This study investigated the effects of an improved extraction method that utilized freeze-thaw, termed improved conventional extraction (ICE), on the yield and properties of the water-soluble non-starch polysaccharide of taro (Tc-WS-NSP) and compared this method to the conventional extraction (CE) method. The freeze-thaw condition was optimized using response surface methodology (RSM) based on yield. The use of the ICE method resulted in a 227.8% increase in yield of Tc-WS-NSP compared to the CE method. The Tc-WS-NSP-ICE had higher purity, lighter color, larger particle size, and higherζ-potential than Tc-WS-NSP-CE. Both of the samples contain the sugar arabinose, galactose, glucose, and mannose and exhibited comparative FTIR, 1H, and 13C NMR spectra. The Tc-WS-NSP-ICE had a semi-crystalline structure resulting in higher thermal stability and had a higher consistency index than Tc-WS-NSP-CE. Overall, the use of the ICE method provided a simple, efficient, and green alternative to CE for the extraction of Tc-WS-NSP.

Extraction, structural elucidation and immunostimulating properties of water-soluble polysaccharides from wheat bran.

A water-soluble polysaccharide was extracted from wheat bran (WBP) and investigate their structural characteristics and immunostimulatory activities. The chemical composition of WBP and purified fraction (WBP-F) mainly consists of neutral sugars (91.2±1.2 and 98.7±1.2%), proteins (8.6±0.3 and 0.2±0.1%) and uronic acids (0.7±0.1 and 0.6±0.1%). The molecular weight (Mw ) of WBP and WBP-F was calculated as 911.7 and 510.2×103 g/mol, respectively. The WBP-F stimulates the RAW264.7 cells through the production of nitric oxide and various cytokines. The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways. Furthermore, the structural details of WBP-F were studied by GC-MS and NMR spectrum, which confirms that the main backbone consists of 4-α-D-linked glucopyranosyl residues with branching points at C-6. PRACTICAL APPLICATIONS: Wheat bran is a potential source of health-promoting compounds. It has been reported that polysaccharides of wheat bran containing numerous beneficial activities. In this study, the wheat bran polysaccharide was extracted, fractionated and investigated their immunostimulatory activities. The results found in this study revealed that the purified polysaccharide from wheat bran potentially enhanced the RAW264.7 cells activation. Hence, these polysaccharides could be utilized as a potent immunity-enhancing agent in food and pharmaceutical industries.

Ultrasound-assisted extraction and antioxidant activity of polysaccharides from Acanthus ilicifolius

In the present study, ultrasound-assisted extraction of water-soluble polysaccharides from the Acanthus ilicifolius was optimized by using one-factor-at-a-time and Box–Behnken designs. A maximum polysaccharide yield of 2.46% was obtained under the optimized conditions of ultrasonic power of 150 W, temperature of 53 °C, extraction time of 50 min, and liquid to solid ratio of 35 mL/g. Results of UV, FTIR and molecular weights indicated that there were significant differences in structures of polysaccharides extracted with ultrasound assistance (UAE-ACI) and hot-water extraction (HWE-ACI) including monosaccharide compositions and ratios, and molecular weight distributions. Further analysis showed that UAE-ACI had a higher antioxidant activity in vitro than that of HWE-ACI possibly due to lower molecular weight and higher content of phenolics and protein fractions.

Extraction and chemical characterization of novel water-soluble polysaccharides from two palm species and their antioxidant and antitumor activities

ABSTRACT Two crude heteropolysaccharides were isolated from fruits of Caryota mitis and Chamaerops humilis in percentages of 2.65 and 3.89, respectively. In the case of Caryota mitis (CM) polysaccharide, it consisted of galactose, glucose and mannose as the predominant monosaccharides. While mannose, galactose and arabinose were respectively the predominant monosaccharides in the structure of Chamaerops humilis (CH) crude polysaccharide. Sixteen common amino acids were recognized to be components of the protein-bound polysaccharides of CM and CH. The major amino acid in CM and CH polysaccharides was tyrosine (1.091ng/g) and arginine (0.684 ng/g), respectively. Antioxidant activities of tested polysaccharides were examined by measuring its scavenging ability on 2,2-diphenyl-1-picrylhydrazyl and ferric reducing activity power assay. In a concentration of 1 mg/ml, CM and CH polysaccharides recorded FRAP values of 0.56 and 0.44 µmol Fe++/g, respectively. The scavenging activity of CM sample was higher (IC50, 422.11 µg/ml) than that of CH polysaccharide (IC50, 630.0 µg/ml). Also, the antitumor activity of CM and CH polysaccharides was investigated using HepG2 and MCF-7 human cell lines. The polysaccharide of Caryota mitis had a higher antitumor activity than that of CHPs; it had IC50 values of 13.6 and 46.4 µg/ml in HepG2 and MCF-7 assays, respectively.

Optimization of ultrasonic-assisted extraction, refinement and characterization of water-soluble polysaccharide from Dictyosphaerium sp. and evaluation of antioxidant activity in vitro

Microalgae have been reported in many studies with high biomass, high carbohydrate content, fast growth rate and multiple bio-activities, while Dictyosphaerium sp. was rarely studied. Polysaccharide prove to be remarkable in many biological activities. To optimize the effects of the ultrasonic-assisted extraction parameters on the yield of polysaccharide from Dictyosphaerium sp., a single-factor experiment and response surface methodology (RSM) with a central composite rotatable design were employed. Four independent variables were investigated: X1 (ratio of raw microalgal powder to water), X2 (extraction temperature), X3 (extraction time), X4 (extraction power). The optimal conditions obtained via single-factor experiment were: X1 (1:25), X2 (50 °C), X3 (50 min), X4 (500 W), while according to the analysis of RSM, the optimum conditions were: X1 (1:26), X2 (58 °C), X3 (55 min), X4 (525 W). The refined polysaccharide were further prepared and characterized using SEM, FT-IR, XRD and TGA/DSC. Five kinds of antioxidant assays were carried out and the results indicated that the refined polysaccharide had strong scavenging ability to DPPH· and OH·. This study provides theoretical and experimental basis for the development and utilization of microalgal resources.

Extraction, purification and characterization of water-soluble polysaccharides from green walnut husk with anti-oxidant and anti-proliferative capacities

Abstract This work was designed to study the physicochemical properties, and bioactivity of green walnut husk polysaccharides (GWHP). The crude GWHP was fractionated into three fractions, namely, GWHP-1, GWHP-2 and GWHP-3, using Cellulose DEAE-52 and Sephadex G-150. HPLC analysis showed that the three fractions were heteropolysaccharides consisting primarily of glucose, galactose and arabinose with average molecular weights of 165.06, 179.64 and 255.75 kDa, respectively, and that the monosaccharide composition of GWHP-1 was more complicated than those of GWHP-2 and GWHP-3. The primary structures of the GWHP fractions were similar to each other. GWHP-1, obtained from water elution, showed higher anti-oxidant activities in the tested concentration range of 0.1–1.8 mg/mL than did GWHP-2 and GWHP-3, which were obtained by elution with 0.1 M and 0.2 M NaCl solutions. Additionally, the GWHP-1 fraction showed the most effective anti-proliferative activity among the three fractions, which was associated with the weakened cell proliferation and increased lactate dehydrogenase (LDH) leakage of LoVo cells. However, GWHP-1 displayed a lower growth inhibition effect on colonic NCM640 cells, which was characterized by low LDH leakage. The above results indicated that the polysaccharides, especially GWHP-1, may be a valuable natural anti-oxidant and anti-cancer cell source for applications in the pharmaceutical and food industries.

Extraction, purification and properties of water-soluble polysaccharides from mushroom Lepista nuda.

Lepista nuda has high nutritional and medicinal value, especially has high antioxidant, antitumor and antiviral activities. Based on results of single factor experiment, response surface methodology was used to optimize the extraction conditions of polysaccharides (LNP). Macroporous resin D301 was used to decolorization, and its technological conditions were determined by orthogonal experiment. In addition, two novel polysaccharides (LNP-1, LNP-2) were purified by DEAE Cellulose-52 and Sephadex G-150 chromatography. The molecular weight of LNP-1 is 11,703Da, mainly composed of mannose, glucose, galactose, xylose, arabinose and fucose, while the molecular weight of LNP-2 is 13,369Da, mainly composed of mannose, glucose, galactose, arabinose and fucose. The polysaccharide contents of crude LNP, LNP-1 and LNP-2 were 70.60%, 87.71% and 81.20% respectively, and the protein contents were 1.72%, 0.97% and 0.68% respectively. Their sulfuric contents were 3.39%, 5.02% and 8.64%, and uronic acid contents were 3.66%, 5.56% and 6.80%, respectively. Further studies showed that their ability to chelate iron ions, scavenge DPPH (1,1‑diphenyl‑2‑picrylhydrazyl) free radicals and scavenge superoxide anion radicals were 70.09%, 55.94% and 36.64% respectively. They showed strong ability to scavenge free radicals in a concentration-dependent manner. LNP is expected to be developed as a new efficacy factor in the food industry.

DEVELOPMENT AND VALIDATION OF THE EXPRESS TECHNIQUE OF ALLOCATION AND QUANTITATIVE DEFINITION OF WATER-SOLUBLE POLYSACCHARIDES OF LEAVES OF ARCTIUM LAPPA L.

Research objective was development of an express technique of allocation and quantitative definition of water-soluble polysaccharides in leaves of a Arctium lappa L. For acceleration of process of extraction of biologically active agents of leaves of a Arctium lappa and also augmentations of an exit of water-soluble polysaccharides, it was decided to use an ultrasonic bathtub. Varying process indicators, it was succeeded to pick up extraction conditions under which process of extraction and quantitative definition of water-soluble polysaccharides of leaves of a Arctium lappa comes down to 1.5 h, namely: an izmelchennost of raw materials of 0.5–1.0 mm, temperature – 80 °С, frequency rate of extraction – 3, duration of extractions – 15 min, ultrasound frequency – 35 kHz, a ratio of raw materials and the ekstragent of 1 g for 15 ml. The developed technique of a validirovan on indicators of repeatability, correctness, fastness and linearity therefore it is proved that it is a pretsizionna in the conditions of repeatability, is correct, steady and has rather rigid linear dependence of mass of a deposit on mass of the analyzed raw materials at gravimetric definition of water-soluble polysaccharides in leaves of а Arctium lappa. The technique can be used for the express analysis of quality of leaves of а Arctium lappa and also at industrial receiving an inulin from this type of raw materials.

Structural viscoelasticity of a water-soluble polysaccharide extract

Two structural changing processes are incorporated into a traditional viscoelastic model to characterize and predict the shear thickening rheological behaviors of a polysaccharide mucilage of black tree fern. The shear-dependent structure effect could cause varying linear viscoelasticity, and the relaxation spectrum in the model was modified by an empirical sigmoidal function and a structure parameter. The steady shear viscosity of the mucilage was fitted to obtain the parameters in the empirical function. The step rate experimental data was fitted to obtain the structural formation and disruption information. According to the varying spectrum in steady shear state, the extract shows slight shear strengthening of linear viscoelasticity at low shear rate, then steep strengthening in 3–6 s−1, and last large strengthening at the shear rate higher than 10 s−1. Both the time-dependent shear thickening viscosity and the shear hysteresis behavior of the mucilage can be predicted using the developing structure.

Improved in vitro antioxidant and antimicrobial capacities of polysaccharides isolated from Salicornia arabica

A newly profiling of Salicornia arabica (SAPS) polysaccharides was investigated. The water-soluble polysaccharide extract extracted from the Tunisian halophyte (SA) contained sulfate (9.64 ± 0.003%), carbohydrate (86.33 ± 0.003%), protein (1.24%), and uronic acids (3.33 ± 0.37%). The analysis of the monosaccharide composition revealed the presence of arabinose, Glucopyranose galactose, Glucuronic acid and glucose. The identified polysaccharide was considered as heterogeneous and highly branched via gaz chromatography, Fourier Transform Infrared Spectrometer (FT-IR), Thin layer chromatography (TLC) analysis. It also displayed an important radical scavenging and reducer power as well it exhibited effective antimicrobial properties.

Anti-Cancerous Effect of Inonotus taiwanensis Polysaccharide Extract on Human Acute Monocytic Leukemia Cells through ROS-Independent Intrinsic Mitochondrial Pathway.

Acute leukemia is one of the commonly diagnosed neoplasms and causes human death. However, the treatment for acute leukemia is not yet satisfactory. Studies have shown that mushroom-derived polysaccharides display low toxicity and have been used clinically for cancer therapy. Therefore, we set out to evaluate the anti-cancerous efficacy of a water-soluble polysaccharide extract from Inonotus taiwanensis (WSPIS) on human acute monocytic leukemia THP-1 and U937 cell lines in vitro. Under our experimental conditions, WSPIS elicited dose-dependent growth retardation and induced apoptotic cell death. Further analysis showed that WSPIS-induced apoptosis was associated with a mitochondrial apoptotic pathway, such as the disruption of mitochondrial membrane potential (MMP), followed by the activation of caspase-9, caspase-3, and PARP (poly(ADP-ribose) polymerase) cleavage. However, a broad caspase inhibitor, Z-VAD.fmk, could not prevent WSPIS-induced apoptosis. These data imply that mechanism(s) other than caspase might be involved. Thus, the involvement of endonuclease G (endoG), a mediator arbitrating caspase-independent oligonucleosomal DNA fragmentation, was examined. Western blotting demonstrated that WSPIS could elicit nuclear translocation of endoG. MMP disruption after WSPIS treatment was accompanied by intracellular reactive oxygen species (ROS) generation. However, pretreatment with N-acetyl-l-cysteine (NAC) could not attenuate WSPIS-induced apoptosis. In addition, our data also show that WSPIS could inhibit autophagy. Activation of autophagy by rapamycin decreased WSPIS-induced apoptosis and cell death. Taken together, our findings suggest that cell cycle arrest, endonuclease G-mediated apoptosis, and autophagy inhibition contribute to the anti-cancerous effect of WSPIS on human acute monocytic leukemia cells.

Extraction optimization, purification and characterization of polysaccharides from the seed coat of black soybean.

In this study, the extraction of water-soluble polysaccharides from the seed coat of black soybean (BSCP) was investigated and optimized. A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction temperature, 100°C; ratio of water to material, 22.3 mL/g; and extraction time, 133.2 min. Under these conditions, the experimental yield of polysaccharides was 10.56%, which was consistent with the predictive yield. A novel galactomannan, BSCP-1, with a molecular weight of 7.55 × 105 Da determined by high-performance gel permeation chromatography, was isolated from the black soybean seed coat. Through gas chromatography-mass spectrometry analysis, BSCP-1 was identified as a galactomannan consisting of galactose, mannose and rhamnose in a molar ratio of 6.01:3.56:1.00. Cytotoxicity against the human gastric carcinoma cancer cell line was also determined.

Effect of water-soluble polysaccharide extract from the green alga Ulva rigida on growth performance, antioxidant enzyme activity, and immune stimulation of grey mullet Mugil cephalus

This study was carried out to investigate and compare the effect of water-soluble polysaccharides extract of the green alga, Ulva rigida (WPU), as a dietary supplement on growth performance, antioxidant enzyme activity, non-specific immune responses, and disease resistance of juvenile grey mullet, Mugil cephalus, subjected to bacterial infection with Photobacterium damselae. Three replicate groups of fish (initial mean weight, 15 ± 0.1 g) were fed with four isonitrogenous and isolipidic diets containing four levels, 0 or control, 5, 10, and 15 mg kg−1 of WPU (WPU0 or control, WPU5, WPU10, and WPU15) for 8 weeks over the growth trial. Thereafter, ten fish from each dietary treatment were infected with bacteria P. damselae to evaluate disease resistance of infected fish. Feeding grey mullet at 10 mg kg−1 WPU level (WPU10 diets) produced higher weight gain (211.36 ± 6%), specific growth rate (1.88 ± 0.03%) and protein efficiency ratio (4.26 ± 0.1%) compare to the control group. The diets supplemented with three levels of WPU-stimulated lysozyme, phagocytic, and respiratory burst activity of fish, but, the stimulatory capacities of the extracts varied greatly, depending on the concentrations used and diet; WPU10 was higher than WPU 5 and WPU15 diets. The best responses in antioxidant enzyme activities of superoxide dismutase (4.8 U mL−1), glutathione (3.6 U mL−1), and malondialdehyde (25.0 U mL−1) were induced by the extracts obtained from WPU10 diet. In addition, using WPU extract in all diets decreased mortality in M. cephalus in a dose-dependent manner after challenge with P. damselae. These results suggest that the use of water-soluble polysaccharides from U. rigida at 10 mg kg−1 dose improve growth, immune responses, and antioxidant activity in M. cephalus.

Polysaccharides fromEnteromorpha tubulosa: Optimization of extraction and cytotoxicity

An ultrasound-assisted procedure for the extraction of water-soluble polysaccharides from Enteromorpha tubulosa was studied by using response surface methodology. Three independent variables including extraction time, extraction temperature, and ratio of liquid to raw material were investigated by single-factor experiments. A three-factor-three-level Box–Behnken design was adopted to optimize the extraction process parameters. A highly significant second-order polynomial equation was fitted by using multiple regression analysis. The optimal extraction conditions were obtained as follows: extraction time 36 min; extraction temperature 69 °C; and ratio of liquid to raw material 45 mL/g. By experiments with these conditions, the extraction yield was 16.04%, which was well matched with the predicted value. The obtained polysaccharides were evaluated for cytotoxicity in vitro against human breast cancer cell line MCF-7 and the results showed that the polysaccharides possessed potent cytotoxicity. Practical applications Seaweeds are one of the largest numbers and species of marine plants, and their bioactive compounds, especially algal polysaccharides, have become the focus of research in the field of food and medicine. Moreover, many algal polysaccharides have been gradually applied to food and medicine owing to their varied biological activities, such as antivirus, antitumor, antiradiation, antimutation, antioxidation, and enhancing immunity. However, there is no universal extraction process for polysaccharides from the different seaweeds because of the different structures of the seaweeds and the varying compositions of algal polysaccharides. In the current research, an ultrasound-assisted procedure for the extraction of water-soluble polysaccharides from Enteromorpha tubulosa was studied by using response surface methodology, and the optimal extraction conditions were obtained so as to provide references for the mass productions in the factories.

Amylase-assisted extraction and antioxidant activity of polysaccharides from Gracilaria lemaneiformis.

Gracilaria lemaneiformis (G. lemaneiformis) is rich in polysaccharides that have many functional activities. In this study, the thermostable α-amylase-assisted extraction and antioxidant activity of water-soluble polysaccharides from G. lemaneiformis were investigated. The extraction conditions were optimized as follows: time, 40min; temperature, 95°C; pH, 5 and enzyme amount, 6000U/g, under which the yield of G. lemaneiformis polysaccharides (GPs) reached 49.15% dry base. The GPs were characterized by monosaccharide composition, FTIR spectrum, UV spectrum and antioxidant activities. The results indicate that the GPs had strong hydroxyl radical activity at a concentration of 100μg/mL and could be used as potential antioxidants.

Improved Extraction and Quality Characterization of Water‐Soluble Polysaccharide from Gamma‐Irradiated Lentinus edodes

Gamma irradiation was applied to the improved extraction of water-soluble polysaccharides (WSPs) from dried Lentinus edodes. Irradiation provided a dose-dependent increase in extraction yield (0kGy, 2.01%; 7.5kGy, 4.03%; 15kGy, 7.17%) and purity (0kGy, 78.8%; 7.5kGy, 83.1%; 15kGy, 85.6%) of the WSPs from hot-water extraction. The effect of irradiation was evident in the degraded microstructures and reduced molecular weights of the WSPs. However, nuclear magnetic resonance, Fourier-transform infrared, and X-ray diffraction spectroscopic analyses provided comparable structures of WSPs from nonirradiated and irradiated samples. UV-visible spectra showed a dose-dependent decline in intensity, but an improvement in thermal properties of the WSPs from the irradiated mushroom samples was observed.

Extraction, characterization and antioxidant activity of water-soluble polysaccharides from Tuber huidongense

Single-factor experiment and Box–Behnken design were employed to optimize the ultrasonic-assisted extraction (UAE) of water-soluble polysaccharides from Chinese truffle Tuber huidongense (THWP). The optimal extraction conditions with an extraction yield of 7.17±0.22% crude THWP were determined as follows: ultrasonic power 99.65W, extraction time 40.39min, ratio of water to raw material 24.65mL/g, and extraction temperature 70.1°C. Two purified fractions, THWP-1 and THWP-2 with molecular weights of 128kDa and 729kDa, respectively, were obtained from crude THWP by Cellulose DEAE-52 and Sephadex G-100 chromatography. Monosaccharide component analysis by high performance liquid chromatography (HPLC) indicated that THWP-1 was composed of Glc, Man, and Gal with their corresponding mole percentages of 60.56%, 20.12% and 19.32%, respectively and that THWP-2 contained only Glc. Evaluation of the antioxidant activity in vitro suggested that THWP-1 and THWP-2 had effective scavenging activity of 2,2′-azinobis-(3-ethylbenzothiazolin-6-sulfonicacid) (ABST), hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Accordingly, the polysaccharides from Chinese truffle might serve as a natural antioxidant in functional foods.

Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya Maxim

In this study, the extraction of water-soluble polysaccharides (BDPs) from Berberis dasystachya Maxim using dynamic microwave-assisted extraction (DMAE) was discussed. A Box–Behnken design combined with response surface methodology has been employed to optimize extraction parameters of DMAE. The BDPs have been analyzed in order to identify a variety of chemical properties. Antioxidant and anti-tumor activities in vitro have been studied by DPPH, ABTS, reducing power assay, and MTT assay, respectively. The results obtained showed that the optimal extraction conditions were as follows: ratio of water to raw material (X1) 25.84mg/L, extraction power (X2) 433.13W, extraction time (X3) 35.18min, and the maximum yield of extraction was 6.472±0.384%, which was in good agreement with the predicted value. The physicochemical tests demonstrated that the BDPs mainly consist of rhamnose, arabinose, xylose, mannose, glucose and lactose in a molar ratio of 1:17.3:1.33:7:2.33:1.78; the average molecular weight of the BDPs was estimated to be from 2.95×105 and 1.52×103Da, respectively. Furthermore, the BDPs exhibited effective antioxidant and anti-proliferative properties in vitro. Such pharmaceutical activities could prove useful for potential future applications involving the berries of B. dasystachya Maxim.

Studies on the steady shear flow behavior and chemical properties of water-soluble polysaccharide from Ziziphus lotus fruit

The extraction of water-soluble polysaccharide from Ziziphus lotus fruit (WPZL) was performed by ultrasonic-assisted extraction method. A Box–Behnken design (BBD) was applied to evaluate the effects of three independent variables (ultrasonic power (X1: 70–100W), extraction time (X2: 10–30min), extraction temperature (X3: 55–95°C), and water to raw material ratio (X4: 5–25)) on the extraction yield of APH. The effect of temperature and concentration on flow behavior of gum solution was studied. WPZL solutions exhibited shear-thinning non-Newtonian flow behavior for concentrations above 0.5% (w/v). The viscosity of fully hydrated gum solutions decreased as temperature increase. The correlation analysis of the mathematical-regression model indicated that quadratic polynomial model could be employed to optimize the extraction of WPZL. The optimal conditions to obtain the highest extraction of WPZL (13.398±0.019%) were as follows: ultrasonic power, 88.77W; extraction time, 29.96min, extraction temperature, 77.73°C and water to raw material ratio 24.44mL/g.

Extraction of water-soluble polysaccharide and the antioxidant activity from Semen cassiae

Water-soluble polysaccharide was isolated from Semen cassiae using water for extraction and ethanol for deposition. The optimized conditions for polysaccharide isolation by orthogonal experiments were a sample to liquid ratio of 1:30 at 80°C for 3.5 hours; the yield of polysaccharide from Semen cassiae under these conditions was 5.46%. Different polysaccharides (SCPW-1, SCPW-2, SCPW-3, SCPW-4, SCPW-5, SCPS-1, SCPS-2) were obtained from the extract (i.e., crude polysaccharide) by DEAE-cellulose column chromatography. The polysaccharides obtained showed different structures by Fourier transform infrared therein the five elected from the seven kinds separated. The antioxidant activities of the extract were evaluated. The scavenging rates of the present extract on hydroxyl and superoxide were 43.32% and 64.97%, respectively, at a concentration of polysaccharide of 94.03 μg/mL, which was better than vitamin C at the same concentration. The scavenging rate of the present extract on 1,1-diphenyl-2-picrylhydrazyl was 13.33% at a polysaccharide concentration of 94.03 μg/mL, which was less than vitamin C at the same concentration.