Root Extract Research Articles

Synthesis and production of new sanitary and hygienic products modified with nanocomposites

The problem of maintaining high quality of manufactured products is crucial. A key factor in the food quality assurance system is industrial sanitation. In this regard, widely used drugs are detergents, but it is necessary to modify existing developments taking into account the concept of greening food production and safety in relation to humans. In addition, the problem is solved by selecting optimal conditions for cleaning process equipment using detergent compositions. To solve this problem, we propose adding additives to detergents that fix toxins and prevent their penetration. Also, to reduce the harmful effects of toxins, licorice root extract will be introduced into detergents as an antidote. The fixing additive is an innovative product – a nanostructured carbon-silicon composite with high sorption capacity. According to available data, the immobilization of biological products on carbonsilicon containing composites is carried out either by adsorption or chemical bonding. The retention of the adsorbed molecule occurs due to the van der Waals bond.

The photoprotective effectiveness of microemulsion gel made from an ethanol extract of kelakai root (Stenochlaena palustris (Brum. f) bedd.)

Background: Free radicals from sunlight have detrimental effects on the skin, such as black and dull colouration and dry- and easy-to-age skin. Prevention can be done by using antioxidants derived from plants as photoprotective. Kelakai root extract has a potent antioxidant activity of 19.06 ppm. Using typical preparations in the form of microemulsion gels protects the skin from free radicals. Objective: This study aimed to determine the photoprotective effectiveness of the microemulsion gel preparation of kelakai root extract. Method: The extraction method adopted maceration with 70% ethanol solvent. The microemulsion was incorporated into a gel dosage form with a concentration of 5%. The effectiveness of the photoprotective gel was measured using UV-Vis spectrophotometry, which included determining the value of the Sun Protector Factor (SPF), erythema transmission percentage (%Te), and pigmentation transmission percentage (%Tp). Result: The results showed that the microemulsion gel of kelakai root extract with a concentration of 80 ppm had an SPF value of 9.816 with a %Te value of 9.591% and a %Tp value of 16.779%. Conclusion: The microemulsion gel of the kelakai root extract has photoprotective effectiveness as a sunscreen in the sunblock category.

Pharmacological Activities of Green Synthesized Silver Nanoparticles and Leaf, Root Extracts of Muntingia calabura

Pharmacological Activities of Green Synthesized Silver Nanoparticles and Leaf, Root Extracts of Muntingia calabura

A network pharmacology-based approach to explore the potentials of Saluang belum (Luvunga sarmentosa) in the male human reproductive system

Background: Saluang belum's root (Luvunga sarmentosa) is one of the local plants in Central Kalimantan and is used by the Dayaknese to increase sexual activity. In previous research, L. sarmentosa root extract compounds have been characterised, but the bioactivity of each on male fertility has not been studied. Objective: To use bioinformatics to study the effects and mechanisms of molecular compounds of L. sarmentosa extract on the biological system of the male fertility complex. Methods: Network pharmacology methods were used to determine target essentially functional proteins in biological system networks. The molecular mechanism of L. sarmentosa compound activity was analysed using molecular docking and dynamic simulation software such as Autodock 4 and MOE. Result: The network pharmacology analysis showed that the Pregnane X receptor (PXR) protein contributed essentially to male reproductive biological functions. Based on the molecular docking simulations of PXR, 6-phenyl-3,4-dihydro-1H-1,4,5-benzotriazocin-2-one was the best. Pharmacological and toxicity parameters also showed that this compound had good characteristics. In addition, the molecular dynamics simulations showed that the best compounds could maintain complex interactions with PXR based on the resulting RMSD values. Conclusion: The compound 6-phenyl-3,4-dihydro-1H-1,4,5-benzotriazocin-2-one had the most potential as a PXR inductor. This compound also had a better activity than the standard inductor.

In vitro antibacterial and antioxidant activity of flavonoids from the roots of Tephrosia vogelii: a combined experimental and computational study.

Tephrosia vogelii is a traditional medicinal plant used to treat hypertension, diarrhea and urinary disorders. Silica gel chromatographic separation of CH2Cl2/MeOH (1:1) roots extract of T.vogelii afforded seven compounds namely; β-sitosterol (1a), stigmasterol (1b), 6a, 12a-dehydro-deguelin (2), tephrosin (3), maackiain (4), obovatin (5) and 6-oxo, 6a, 12a-dehydro-deguelin (6). GC-MS analysis of essential oils from the root of T.vogelii displayed a total of 17 compounds of which cis-nerolidol (41.7 %) and cadinol (19.7 %) were themajor constituents. CH2Cl2/MeOH (1:1) extract, MeOH extract, maackiain (4) and obovatin (5) showed moderate inhibitory activity against Pseudomonas aeruginosa with MIC value of 0.5, 0.66, 0.83 and 0.83 mg/mL, respectively, compared to ciprofloxacin (MIC of 0.078 μg/mL). 6a, 12a-dihydro-deguelin (2), and 6-oxo, 6a, 12a-dehydro-deguelin (6) displayed significant activity against S.epidermis with MIC values of 0.66 mg/mL. Tephrosin (3) and maackiain (4) also showed moderate antibacterial activity against Staphylococcus aureus and Proteus mirabilis with MIC values of 0.83 and 0.5 mg/mL, respectively, compared to ciprofloxacin (0.312 μg/mL). The radical scavenging activity results indicated that tephrosin (3), obovatin (5) and 6-oxo, 6a, 12a-dehydro-deguelin (6) showed potent DPPH scavenging activity with IC50 values of 10.97, 10.43 and 10.73 μg/mL, respectively, compared to ascorbic acid (IC50 of 5.83 μg/mL). The docking prediction results revealed that 6a, 12a-dehydro-deguelin (2) displayed the best binding energy of-8.1 kcal/mol towards pyruvate kinase of S.aureus (PDB ID: 3T07) and-7.9 kcal/mol towards P.mirabilis urease (PDB ID: 1E9Y) and DNA gyrase B of Escherichia coli (PDB: 4F86) receptors compared to ciprofloxacin (-7.2 to-8.0 kcal/mol). Maackiain (4) and obovatin (5) displayed the minimum binding energy of-7.9 and-8.2 kcal/mol towards the LasR protein of P.aeruginosa (PDB: ID 2UV) and S.epidermidis FtsZ (PDB: ID 4M8I), respectively. The SwissADME drug-likeness and Pro Tox II toxicity prediction results indicated that compounds (2-6) obeyed Lipinski's rule of five with 0 violations and none of them were found to be hepatotoxic, mutagenic, and cytotoxic, respectively. The invitro assessment results supported by the insilico analysis revealed that crude extracts and isolated compounds showed promising antibacterial and antioxidant activity, which proves the therapeutic potential of the roots of T.vogelii.

Capturing the antimicrobial profile of Paeonia officinalis, Jasminum officinale and Rosa damascene against methicillin resistant Staphylococcus aureus with metabolomics analysis and network pharmacology

In the current study, we evaluated the in vitro antibacterial efficacy of the roots’ extracts of Jasminum officinale, Rosa damascene and Paeonia officinalis against MRSA (methicillin-resistant Staphylococcus aureus) by well diffusion technique. The root extract of P. officinalis exerted a potent anti-MRSA with MIC 0.4673 µg/ml, while both J. officinale and R. damascene exhibited very weak activity. Therefore, chemical profiling of the crude extract P. officinalis roots assisted by LC-HR-ESI-MS was performed and led to the dereplication of twenty metabolites of different classes, in which terpenes are the most abundant compounds. On a molecular level, network pharmacology was used to determine the targets of active metabolites to bacterial infections, particularly MRSA. Online databases PubChem, UniProt, STRING, and Swiss Target Prediction were used. In addition to using CYTOSCAPE software to display and analyze the findings, ShinyGO and FunRich tools were used to identify the gene enrichment analysis to the set of recognized genes. The results detected the identified metabolites were annotated by 254 targets. ALB, ACHE, TYMS, PRKCD, PLG, MMP9, MMP2, ERN1, EDNRA, BRD4 were found to be associated with MRSA infection. The top KEGG pathway was the vascular smooth muscle contraction pathway according to enrichment FDR. The present study suggested a possible implication of P. officinalis roots as a potent candidate having a powerful antibacterial activity against MRSA.

Comparative Study of the Phytochemical Profile and Biological Activity of Ajuga reptans L. Leaf and Root Extracts

In this work, the phytochemical composition and the biological activity of the ethanolic extracts obtained from Ajuga reptans L. (Lamiaceae) leaves and roots (growing in Lesser Poland Voivodeship, Poland) were compared. The phytochemical composition of the extracts were determined by the high-performance liquid chromatography with diode-array detection (HPLC–DAD) method. The dominant compounds in both extracts were verbacoside, isoverbacoside, 3,4-dihydroxyphenylacetic acid and rosmarinic acid. The antioxidant capacity of the extracts was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and 2,2’-azobis(3-ethylbenzothiazoline-6-sulfonate (ABTS) tests. The Folin–Ciocalteu assay was used to determine the total polyphenolic content. Additionally, enzyme (tyrosinase, collagenase) inhibition tests and metal chelating ability were studied to assess the anti-aging properties of the extracts. Moreover, the A. reptans extracts’ capacity to absorb the whole range of ultraviolet radiation and high-energy visible (HEV) light was evaluated. The skin irritation test (SIT) EpiDerm was applied to evaluate the safety of the bugle extracts. The noteworthy point is that there is a lack of literature on the assessment of A. reptans root extract activity. Our study is the only one that compares the quantitative composition and biological activity of extracts from the root and leaves of A. reptans. The obtained results indicated that both of the extracts exhibit high antioxidant, chelating and photoprotective activity, but the extract from A. reptans roots showed a higher enzyme inhibition effect for mature skin. The A. reptans root extract, similarly to the leaf extract, could be applied as potentially multi-functional, safe and sensitive cosmetic raw materials, especially in anti-aging and anti-pollution cosmetics.

Formulation and Evaluation of Antibacterial Cream from Mimosa Pudica and Tridax Procumbenz)

The study was carried out to identify through scientific methods the active ingredients and the pharmacological activities of the shoot and root extracts of Mimosa pudica Linn. The Mimosa pudica shoot methanolic extract (MSME),Mimosa pudica root methanolic extract (MRME) showed very good wound healing activity when compared to the standard drug Gentamicin. Tridax procumbens L. (Compositae) is a common weed that grows in the rice fields of India. Traditionally the juice from leaves of Tridax procumbens has been used for healing dermal wound. However, in experimental studies, equivocal pro and anti-healing action of T. Procumbens has been demonstrated. It has been studied previously that stage of wound healing involves acute inflammatory phase followed by the synthesis of collagen and other extra cellular macromolecules, which is later removed to form a scar

A PEWARNA ALAMI AKAR MENGKUDU (Morinda citrifolia L) SEBAGAI PENGGANTI SAFRANIN PADA PEWARNAAN GRAM NEGATIF

Noni is a plant that has the potential to be used as a therapeutic element, but many people are still unaware of its benefits. This plant is one of the herbal plants part of the noni plant has body parts that can almost all be utilised. Noni root or known as Morinda citrifolia L. can be processed and used as a natural dye because it contains morindon. Noni contains the molecule morindin, a disaccharide derivative of anthraquinone or anthraquinone, so its roots are used as a dye. Morindon is a natural dye produced when morindin glycoside is hydrolysed which will produce a red colour. Morinda citrifolia Linn fruit has antibacterial properties inherent in phenol and flavonoid molecules. The purpose of this study is to determine whether or not noni root extract can be used as an alternative to safranin gram staining. The variables used were noni root extract and gram staining which were carried out by Experimental Laboratories. This research began in May to June 2023. The results showed that noni root extract in a 60-second soak could colour gram-negative bacteria but in 20-second and 30-second soaks there was no red colour on gram-negative bacteria. Noni root extract can be applied as an alternative gram stain to safranin.

Photocatalytic degradation of organic dyes and biological potentials of biogenic zinc oxide nanoparticles synthesized using the polar extract of Cyperus scariosus R.Br. (Cyperaceae)

Abstract In this study, the polar root extract of Cyperus scariosus R.Br. was used for the biogenic synthesis of ZnO NPs. The results of this study show that ZnO NPs have a spherical structure with an average size of 85.4 nm. The synthesized catalysts were tested for their photocatalytic activity by degrading methyl orange and methylene blue under sunlight. Improved degradation efficiencies of 79.44% and 84.92% were achieved within 120 min. ZnO NPs exhibited strong antibacterial activity against both Gram-positive Listeria monocytogenes (18 mm) and Staphylococcus epidermidis (20 mm) and Gram-negative strains of Escherichia coli (16 mm) and Bordetella bronchiseptica (14 mm), as shown by the inhibition zones, which were comparable to the positive control (ceftriaxone) but larger than the plant root extract. ZnO NPs showed high antioxidant activity, as a ferric-reducing antioxidant power assay value of 66.29 µg (AAE µg·mL−1) and a DPPH value of 57.44 µg (AAE µg·mL−1) were obtained at a concentration of 500 µL, which was higher than those of the C. scariosus root extract. Quantification of the total phenolic and flavonoid content yielded values of 57.63 µg (GAE µg·mL−1) and 70.59 µg (QCE µg·mL−1), respectively. At a concentration of 500 μL (1 mg·mL−1), the tested nanoparticles (NPs) showed a greater anti-inflammatory effect (84.12%) compared to the root extract of C. scariosus (34.39%). Overall, our findings highlight the versatile properties of green synthesized ZnO NPs and demonstrate their potential for environmental remediation and antimicrobial formulations, as well as promising candidates for further investigation in biomedical fields such as drug delivery and therapy.

Free Radical Scavenging Effect and Immunomodulatory Activity of Total Saponins Extract of Ginseng Fibrous Roots.

Ginseng (Panax ginseng C.A. Mey) is known for its rich saponin compounds and tonic effects. To better utilize the medicinal value of ginseng, this study investigated the extraction process, components, free radical scavenging ability, and immunomodulatory activity of total saponins of ginseng fibrous roots. The response surface methodology was employed to optimize the extraction process of total saponins, and Q-Orbitrap high-resolution liquid chromatography-mass spectrometry (LC-MS) was used to identify the chemical constituents in the total saponins extract of ginseng fibrous roots (GRS). The results showed that the optimal extraction process was achieved with an ethanol concentration of 68%, a material-solvent ratio of 1:25 mL/g, and an extraction time of 20 min, yielding a total saponin content of 6.34% under these conditions. The extract contained four terpenoid compounds and four polyphenolic compounds. GRS exhibited considerable scavenging activity against DPPH and ABTS radicals, with IC50 values of 0.893 and 0.210 mg/mL, respectively. Moreover, GRS restored immune suppression in mice by increasing white blood cell, red blood cell, and neutrophil counts, and improving the lymphocyte. It also promoted immune system recovery, as evidenced by elevated serum levels of IL-2, IFN-γ, TNF-α, and IL-1β in mice. GRS is a natural compound with promising potential for developing antioxidants and immunomodulatory foods.

Residual enamel removal to improve outcomes of mandibular third molar coronectomy: A single-center retrospective cohort study

This study aimed to analyze post-coronectomy complications, chronological root survival rate (success rate) using Kaplan–Meier analysis, and postoperative radiographic signs for root extraction. A total of 555 mandibular third molar coronectomies were clinically and radiologically evaluated (mean follow-up period, 27.2 months; range, 1 month to 10.5 years). Complications were observed in 22 (4.0%) cases. Temporary inferior alveolar nerve damage was observed in one (0.2%) case, and 21 (3.8%) retained roots required extraction between 1 and 64 months after coronectomy due to dry socket (two cases, 0.4%), primary non-wound closure (10 cases, 1.8%), secondary root exposure (seven cases, 1.3%), and submucosal root eruption (two cases, 0.4%). Residual enamel after coronectomy was more significantly found on 13 (61.9%) of 21 extracted roots than on 30 (5.6%) of 534 surviving roots. Kaplan–Meier analysis revealed an overall survival rate of 93.8% at 5 years and 92.2% at 10 years. The 5-year survival rates differed significantly (p < 0.001) between the enamel-free (97.0%) and residual enamel-attached (58.3%) roots. The Cox proportional hazards model showed a hazard ratio of 20.87 (95% confidence interval, 8.58–50.72). The long-term outcomes of coronectomy were satisfactory, and a higher success rate is expected when the enamel is completely removed during coronectomy.

Rheum khorasanicum Decreases Migration and Induces Apoptosis in the MDA-MB-231 Breast Cancer Cell Line

aims: N/A background: Rheum khorasanicum belongs to the Polygonaceae family, which is well-known for its anticancer activities. objective: Due to the limited studies on the anticancer activity of R. khorasanicum, the present study aimed to evaluate the apoptotic effect of this medicinal plant on MDA-MB-231 cells as a model for an aggressive triple negative breast cancer that has lower treatment option. method: The high-performance thin layer chromatography (HPTLC) assay was used for determining the main ingredients of the extract. Viability and apoptosis of the cells was evaluated by WST-1 and the annexin-V/PI dual staining assay, respectively. The scratch assay was used to assess the cellular migratory potential, and the western blotting test was employed for determining the expression of proteins involved in the apoptotic pathway. result: The HPTLC analysis indicated that emodin, gallic acid, epicatechin gallate, and rhaponticin are pharmacologically active compounds isolated from the hydro-alcoholic extract of R. khorasanicum. The extract showed a significant toxic effect on MDA-MB-231 cells. R. khorasanicum extracts also inhibited cellular migratory potential at concentrations higher than 60 μg/mL. The plant extract significantly diminished the Bcl-2/Bax ratio and increased cleaved caspase- 3, cleaved caspase-7/ procaspase-7 expression at protein levels compared to the nontreated cells. conclusion: Our findings demonstrated that effect root extract efficiently triggered the death of breast cancer cell lines possibly through the stimulation of the apoptotic signaling pathway. other: N/A

Acute and Repeated Ashwagandha Supplementation Improves Markers of Cognitive Function and Mood.

Ashwagandha has been reported to reduce stress and attenuate cognitive decline associated with inflammation and neurodegeneration in clinical populations. However, the effects as a potential nootropic nutrient in younger populations are unclear. This study examined the effects of liposomal ashwagandha supplementation on cognitive function, mood, and markers of health and safety in healthy young men and women. 59 men and women (22.7 ± 7 yrs., 74.9 ± 16 kg, 26.2 ± 5 BMI) fasted for 12 h, donated a fasting blood sample, and were administered the COMPASS cognitive function test battery (Word Recall, Word recognition, Choice Reaction Time Task, Picture Recognition, Digit Vigilance Task, Corsi Block test, Stroop test) and profile of mood states (POMS). In a randomized and double-blind manner, participants were administered 225 mg of a placebo (Gum Arabic) or ashwagandha (Withania somnifera) root and leaf extract coated with a liposomal covering. After 60-min, participants repeated cognitive assessments. Participants continued supplementation (225 mg/d) for 30 days and then returned to the lab to repeat the experiment. Data were analyzed using a general linear model (GLM) univariate analysis with repeated measures and pairwise comparisons of mean changes from baseline with 95% confidence intervals (CI). Ashwagandha supplementation improved acute and/or 30-day measures of Word Recall (correct and recalled attempts), Choice Reaction Time (targets identified), Picture Recognition ("yes" correct responses, correct and overall reaction time), Digit Vigilance (correct reaction time), Stroop Color-Word (congruent words identified, reaction time), and POMS (tension and fatigue) from baseline more consistently with several differences observed between groups. Results support contentions that ashwagandha supplementation (225 mg) may improve some measures of memory, attention, vigilance, attention, and executive function while decreasing perceptions of tension and fatigue in younger healthy individuals. Retrospectively registered clinical trial ISRCTN58680760.

PHYTOCHEMICAL SCREENING, ANTIMICROBIAL, AND ANTIOXIDANT ANALYSIS OF BERBERIS LYCIUM ROYLE CRUDE ROOT EXTRACTS

Objective: This particular study was designed to evaluate the qualitative, quantitative, anti-oxidant, and antimicrobial potential of Berberis lycium root extracts. Methods: The plant root extracts were prepared using a sequential extraction method using different solvents with increasing polarity. Further extracts were evaluated qualitatively for the presence of different phytochemicals and quantitatively for the presence of total phenols and flavonoid, antioxidant potential using DPPH, ferric reducing anti-oxidant power, and H2O2 radical scavenging assay. Biological potential of these extracts was established for antibacterial effect against different Gram-negative and Gram-positive bacteria and antifungal potential against fungal strains. Results: Qualitative analysis of the root extracts showed the presence of phytochemicals such as saponins, tannins, phenols, and flavonoids. Among all the evaluated extracts in quantitative analysis, methanolic extracts showed the highest content of phenolic with 39.75 mg gallic acid equivalence/g and flavanoid with 456 mg QE/g, highest antioxidant potential with half-maximal inhibitory concentration (IC50) value of 10.96 μg/mL in DPPH assay. Similarly, the highest antibacterial effect against Bacillus cereus (20±0.86 mm) and antifungal against Aspergillus niger (IC50 399.64 μg/mL) was observed. Conclusion: The plant root extracts of B. lycium have shown substantial content of phytochemicals with appreciable antioxidant and antimicrobial activities comparable to the currently prescribed modern drugs tested. Accordingly, further studies on clinical efficacy trial, safety, and toxicity analyses have to be instigated promptly, so as to head to the final step to synthesize precursor molecules for new effective antimicrobials.

Survival Rates of Amalgam and Composite Resin Restorations from Big Data Real-Life Databases in the Era of Restricted Dental Mercury Use.

Tooth decay, also known as caries, is a significant medical problem that harms teeth. Treatment is based on the removal of the carious material and then filling the cavity left in the tooth, most commonly with amalgam or composite resin. The consequences of filling failure include repeating the filling or performing another treatment such as a root canal or extraction. Dental amalgam contains mercury, and there is a global effort to reduce its use. However, no consensus has been reached regarding whether amalgam or composite resin materials are more durable, and which is the best restorative material, when using randomized clinical trials. To determine which material is superior, we performed a retrospective cohort study using a large database where the members of 58 dental clinics with 440 dental units were treated. The number of failures of the amalgam compared to composite resin restorations between 2014 and 2021 were compared. Our data included information from over 650,000 patients. Between 2014-2021, 260,905 patients were treated. In total, 19,692 out of the first 113,281 amalgam restorations failed (17.49%), whereas significantly fewer composite restorations failed (11.98%) with 65,943 out of 555,671. This study indicates that composite is superior to amalgam and therefore it is reasonable to cease using mercury-containing amalgam.

Cancer therapeutic potential of hovetrichoside C from Jatropha podagrica on apoptosis of MDA-MB-231 human breast cancer cells

Phytochemical analysis of the methanolic extracts of Jatropha podagrica stalks and roots using liquid chromatography-mass spectrometry (LC-MS) led to the isolation of six compounds: corchoionoside C (1), isobiflorin (2), fraxin (3), hovetrichoside C (4), fraxetin (5), and corillagin (6). The isolated compounds (1–6) were tested for their cytotoxicity against MDA-MB-231 human breast cancer cells. Remarkably, compound 4 (hovetrichoside C) exhibited robust cytotoxicity against MDA-MB-231 cells, displaying an IC50 value of 50.26 ± 1.22 μM, along with an apoptotic cell death rate of 24.21 ± 2.08% at 100 μM. Treatment involving compound 4 amplified protein levels of cleaved caspase-8, -9, -3, -7, BH3-interacting domain death agonist (Bid), Bcl-2-associated X protein (Bax), and cleaved poly (ADP-ribose) polymerase (cleaved PARP), while concurrently reducing B-cell lymphoma 2 (Bcl-2) levels. In totality, these findings underscore that hovetrichoside C (4) possesses anti-breast cancer activity that revolves around apoptosis induction via both extrinsic and intrinsic signaling pathways.

Phytochemical Screening, Acute Toxicity and Evaluation of in vitro Antiurolithaitic Activity of Ethanolic and Methanolic Root and Leaf Extracts of Acalypha indica Linn.

Phytochemical Screening, Acute Toxicity and Evaluation of in vitro Antiurolithaitic Activity of Ethanolic and Methanolic Root and Leaf Extracts of Acalypha indica Linn.

Phytomediated synthesis of Fe3O4 nanoparticles using Cannabis sativa root extract: photocatalytic activity and antibacterial efficacy

Phytomediated synthesis of Fe3O4 nanoparticles using Cannabis sativa root extract: photocatalytic activity and antibacterial efficacy

Accelerating healing of infected wounds with G. glabra extract and curcumin Co-loaded electrospun nanofibrous dressing.

This study aimed to construct a nanofibrous wound dressing composed of polyvinyl alcohol (PVA) and chitosan (CS) containing curcumin and Glycyrrhiza glabra root extract to inhibit infection and accelerate wound healing. Loading 10wt% of G. glabra extract-curcumin (50:50) by electrospinng technique resulted in the formation of nanofibers (NFs) with diameter distribution 303 ± 38 and had a uniform and defect-free morphology. FTIR analysis confirmed the loading of the components without adverse interactions. Also, the results showed extremely high porosity, extraordinary liquid absorption capacity, and complete wettability. In addition, G. glabra extract-curcumin showed significant antioxidant activity and their release profile from NFs was continuous and sustained. Also, the prepared NF could inhibit the growth of both Gram-positive Saureus and Gram-negative E. coli strains. Wound healing evaluation in the infected animal model showed that the NFs caused full wound closure and accelerated skin regeneration. The studies on inhibiting the bacteria growth at the wound site also revealed complete inhibitory effects. Moreover, histopathology studies confirmed the complete regeneration of skin layers, formation of collagen fibers, and angiogenesis. Finally, PVA/CS NFs containing G. glabra extract-curcumin as a multifunctional bioactive wound dressing presented a promising approach for promoting the healing of infected wounds.