Green Bean Extract Research Articles

Administration of arabica coffee green bean extract (Coffea arabica L.) reduces tumor necrosis factor-α levels and increases superoxide dismutase levels in excessive physical exercise male Wistar rats (Rattus norvegicus)

Background: Green Arabica coffee bean water extract contains phenolic acid, polyphenols, caffeine, and chlorogenic acid, which are responsible for health benefits including antioxidant and anti-inflammatory properties. Excessive exercise induces oxidative stress. Tumor necrosis factor-α (TNF-α) and the superoxide dismutase (SOD) may be modified by routine administration of green Arabica coffee bean water extract at the appropriate concentration. Thus, the study aimed to determine the effect of green Arabica coffee bean water extract on TNF-α and SOD levels after excessive physical exercise treatment in a male Wistar rat.Methods: This research used a posttest-only control group design method. Subjects were Wistar rats (Rattus norvegicus), male, 2-3 months old, weighing 150-200 grams. The P0 group was not given treatment. Groups P1, P2, P3, and P4 were given excessive physical exercise. Group P1 was given distilled water. Groups P2, P3, and P4 were given Arabica coffee bean extract at 200, 400, and 800 mg/kg, respectively. Phytochemical was determined by thin layer chromatography (TLC) spectrophotometry method for caffeine, but phenolic acid and polyphenol were standardized to gallic acid (GA). Antioxidant activity was tested using the DPPH method. TNF-α and SOD levels were determined by ELISA methods. Differences among groups were tested with one-way ANOVA and least significant difference (LSD) post hoc test at p < 0.001.Results: One-way ANOVA test showed differences in TNF-α levels (F = 6,388.964; p < 0.001). The LSD post hoc test showed that TNF-α levels in the P1 (159.640 ± 0.167) were higher compared to the P0 (151.720 ± 0.303) (p < 0.001). The levels of TNF-α in P2 (139.680 ± 0.303) were lower than in P1 (p < 0.001). However, TNF-α levels in P3 (161.680 ± 0.084) and P4 (163.750 ± 0.412) were higher than in P1. One-way ANOVA test showed that there were differences in SOD levels (F = 5,564.526; p = 0.003). The LSD post hoc test showed that SOD levels in the P1 (0.742 ± 0.009) were lower than those in the P0 (1.448 ± 0.022) (p < 0.001). The levels of SOD in P2 (1.668 ± 0.026) were higher than in P1 (p < 0.001). However, SOD levels in P3 (0.680 ± 0.003) and P4 (0.591 ± 0.010) were lower than in P1.Conclusion: Administration of Arabica coffee green bean extract prevents an increase in TNF-α levels and decreases SOD levels in excessive physical exercise male Wistar rats.

Influence Green Bean Extract in Increasing Hemoglobin (Hb) Levels in Trimester II Pregnant Motherat Pratama Clinic of bunda Tessa Dusun 1A Sidourip Sub-District Beringin Deli Serdang Regency in 2023

Introduction: Pregnant women with anemia are still many in Indonesia, including in North Sumatra which reached 39%. Pregnancy is very susceptible to anemia that can cause bleeding in pregnant women. Purpose: The purpose of this study was find out the influence Green Bean extract in increasing Hemoglobin Levels in Trimester II Pregnant Women in Pratama Clinic of Bunda Tessa Dusun IA Sidourip Subdistrict Beringin District Deli Serdang in 2023. Method: Types of Quasy Experiment with the design of One Group Pretest Posttest. The population was 20 people. Sampling technique with purposive sampling was a selection of samples based on certain characteristics as many as 10 people, the statistic test used wilcoxon test. Result: Based on the result showed that the data obtained was pre-test minimum value of 8.4 maximum 11.9 while post-test minimum 10.8 maximum13.7. From the results of statistical tests thatwas with wilcoxon test known value P-value= .11 <. 05. Conclusion: The ability in this study is the effect of green bean extract on increasinghemoglobin levels in trimester II pregnant women in Pratama Clinic of Bunda Tessa Dusun IA Sidourip Subdistrict Beringin District Deli Serdang in 2023. It is suggested to be an evaluation and reference material for health workers to be applied and can inform pregnant women who have anemia in a natural way that is by consuming green bean juice as much as 200 ml.

Synthesis and toxicity assessment of Coffea arabica extract-derived gold nanoparticles loaded with doxorubicin in lung cancer cell cultures.

Cancer is the second leading cause of death worldwide, despite the many treatments available, cancer patients face side effects that reduce their quality of life. Therefore, there is a need to develop novel strategies to increase the efficacy of treatments. In this study, gold nanoparticles obtained by green synthesis with Coffea arabica green bean extract were loaded with Doxorubicin, (a highly effective but non-specific drug) by direct interaction and using commercial organic ligands that allow colloidal dispersion at physiological and tumor pH. Conjugation of these components resulted in stable nanohybrids at physiological pH and a tumor pH release dependent, with a particle size less than 40nm despite having the ligands and Doxorubicin loaded on their surface, which gave them greater specificity and cytotoxicity in H69 tumor cells.

EDUKASI PEMBERIAN SARI KACANG HIJAU (VIGNA RADIATE) SEBAGAI UPAYA PENINGKATAN PRODUKSI ASI PADA IBU NIFAS

Exclusive breastfeeding refers to the practice of providing breast milk solely to infants aged 0-6 months without introducing any additional solid foods or liquids such as formula, fruit juice, honey, or tea. The objective of this study is to assess the impact of administering green bean juice (Vigna Radiate) on enhancing postpartum breast milk production among mothers receiving care at the Yeni Clinic in the Tinggi Raja District, Asahan Regency, in the year 2022. This research follows a quantitative approach and employs a quasi-experimental design known as the "slum experiment," involving both pre-test and One Group post-test designs. Data analysis involved the application of a paired t-test on a sample of 10 postpartum mothers facing challenges in providing exclusive breastfeeding, and the study was conducted in June 2022. The findings of the study indicated that prior to the introduction of green bean extract, the majority of mothers (90%) experienced low breast milk production, with only one mother (10%) achieving sufficient breast milk production. Following the reintroduction of green bean extract, two mothers (20%) observed an increase in breast milk production, while eight other mothers (80%) achieved adequate breast milk production. Data analysis results revealed a p-value of 0.000, which is less than 0.005, indicating a significant influence of administering green bean juice (Vigna Radiate) on breast milk production among postpartum mothers at the Yeni Clinic, Tinggi Raja District, Asahan Regency, in 2022.

The Role of Robusta Green Bean Extract in Decreasing Il-1β Levels on Dental Pulp Stem Cells (Dpsc) Induced by Lps Porphyromonas Gingivalis

Background: Stem cell-based tissue engineering is a potential strategy for periodontal tissue regeneration. Besides the advantages of stem cells as periodontal tissue regenerative therapy, this therapy must also be able to prevent and control the possibility of inflammation that occurs. Robusta green beans contain anti-inflammationsubstances that may decrease excessive inflammation in stem cell therapy. Purpose: To analyze IL-1β levels after giving Robusta green bean extract to DPSC induced by LPS P. gingivalis. Materials and Methods: This study used DPSC from the extraction of premolar teeth of orthodontic patients. The viability test was performed on the LPS P. gingivalis at 0.5; 1; 5; 10µg/ml and robusta green bean extract at 0.0625%; 0.125%; 0.25%; 0.5%to determine non toxic concentration. The treatment group was divided into 4 groups, the control group, the DPSC+LPS group, the DPSC + LPS + 0.125% Robusta green bean extract group, and the DPSC+LPS+0.0625%Robusta green bean extract group.Each group was observed at 24, 48, and 72 hours. ELISA test was performed on the supernatant to measure IL-1β levels. Results: The level of IL-1β in the Robusta green bean extract group was significantly lower (p <0.05) than the group without Robusta green bean extract at all observation times. Conclusion: Robusta green bean extract concentrations of 0.125% and 0.0625% can significantly reduce IL-1β levels in DPSC induced by LPS P. gingivalis, which is the most effective at reducing IL-1β levels was 0.125%.

The Influence of Giving Green Bean Extract and Bean Extract Soybean on Increasing Haemoglobin Levels in Trimester III Pregnant Women

Anemia is a health problem throughout the world, especially in developing countries. Iron deficiency problems often cause anemia in pregnant women because iron is needed to help the body produce new red blood cells rich in oxygen and nutrients; the impact of anemia on pregnant women is not insignificant. Mainly, pregnant women who experience anemia in pregnancy will affect the period of pregnancy, labor, and childbirth, namely miscarriage, bleeding, premature parturition, uterine inertia, prolonged delivery, uterine atony, shock, stillbirth, in the puerperium, and can even have an impact on death. Aim the study to measure the increase in hemoglobin levels in third-trimester pregnant women from the effect of green bean and soybean extracts.
 This research is a quasi-experimental research with a two-group pre-test - post-test approach. The population of this study was the third-trimester pregnant women who had their pregnancies checked at the Putri Ayu Public Health Centre. The sampling technique for this study was simple random sampling, and the sample size was 20 respondents who met the inclusion criteria. The sample used in this study is simple random sampling. Following the research hypothesis described, the statistical analysis technique used is univariate with frequency distribution and bivariate with the Wilcoxon test. Research analysis was carried out with the help of a computerized program. Based on the study's Wilcoxon test results, the green bean group value was ρ = 0.003 (CI 95%), while in the soybean group, it was ρ = 0.011. This means that mung bean extract is more influential in increasing haemoglobin levels in thirdtrimester pregnant women than soybean extract.

ANTIBACTERIAL ACTIVITY OF JAVA PREANGER GEL FROM CHERY COFFEE EXTRACT AND GREEN BEAN COFFEE (Coffea arabica L.) AS ANTI-ACNE

Acne is a skin condition marked by nodules, papules, and pustules. Antibiotic treatment for acne frequently results in resistance. Meanwhile, the usage of various anti-acne medications produces skin irritation. Coffee arabica (Coffea arabica L.) Java preanger has a chlorogenic acid molecule with antibacterial properties against Propionibacterium acnes. This study aimed to examine the antibacterial activity of coffee cherry seed extract and green bean arabica coffee (Coffea arabica L.) Java preanger to determine which had the superior anti-acne action. The gel composition had 2% carbopol and 3%, 6%, and 9% coffee cherry extract concentrations, as well as 5%, 10%, and 15% coffee green bean extract concentrations. The obtained formulations were tested for physical and antibacterial efficacy against Propionibacterium acne. The physical evaluation of the formulations included organoleptic testing, homogeneity, pH, viscosity, spreadability, and antibacterial activity tests. The formulations that met the quality requirements for cherry coffee and coffee green bean gel were selected based on the findings of the physical evaluation. While formula 3 at a concentration of 9% coffee cherry seed extract has the best antibacterial activity with an inhibition of 15.27 mm, green bean coffee at 15% has an inhibition of 17.30 mm.

Aromatic Yeasts: Interactions and Implications in Coffee Fermentation Aroma Profiles.

Nontraditional yeasts prevalent in tropical agricultural fermentations such as coffee and cocoa are known to contribute to aroma profiles, yet the functional roles and interactions between the associated microbial consortia in a farm fermentation are unclear. Here, boiled green bean extract (GBE) from green coffee beans was developed as a rich screening medium to deconstruct the microbial consortia and their interactions during the fermentation of dried green coffee beans. When cultivated in coculture with S.cerevisiae on GBE, strain-specific groupings with distinct volatile organic profiles were observed for nontraditional yeasts (e.g., Hanseniaspora spp., Pichia kudriavzevii). Further changes are evident when constructed consortia composed of nontraditional yeast, S.cerevisiae, and Lactococcus lactis var. cremoris were cultured in GBE, and a comparison with abiotically acidified GBE suggests that pH plays a major role in the influence of lactic acid bacteria (LAB) on fermentation aromas. This approach represents a tool for the development of starter culture formulations to create different flavor profiles in coffee fermentation.

Perbandingan Konsumsi Buah Naga dan Sari Kacang Hijau terhadap Peningkatan Kadar Hb pada Ibu Hamil Anemia di Wilayah Puskesmas Mranggen II Kecamatan Mranggen Kabupaten Demak

ABSTRACT 
 Background: Anemia is still a complex health problem in Indonesian women. The most common cause of anemia in pregnancy is lack of nutrients in the body. Pharmacological treatment has side effects faster than non-pharmacological treatment. Natural products that are traditionally used as an additional intake in the prevention of anemia, for example there are dragon fruit, green beans, and so on. 
 Purpose: The purpose of this study was to determine the effectiveness comparison between consumption of dragon fruit and mung bean juice on increasing Hb levels in anemia pregnant women in the Mranggen II Public Health Center, Demak Regency. 
 This type of research is quantitative research with a research design using Two Group Pretest Posttest Design. The population in this study were pregnant women with anemia. Sampling using the entire population of as many as 40 respondents. Giving dragon fruit in the form of pieces shows 100% of the ingredients used and green beans are made by boiling to get the juice from the green beans. The research instrument was the Easy Touch Hb test as a measuring tool for Hb levels. Data analysis used Paired T-Test / T-dependent test. 
 Result: The results showed that the average hemoglobin level of pregnant women with anemia in the intervention group before being given Dragon Fruit had a Mean value of 10.241 g/dl, while after the intervention the Mean value became 11.125 g/dl. The Hb level in the control group before being given Mung Bean Extract had a Mean value of 10.825 g/dl while after being given Mung Bean Extract the Mean value became 11.266 g/dl. The results of the statistical test using the Independent T-Test between the two intervention groups and the control group showed p-values 0.539 which means there is no difference in the effectiveness of giving Dragon Fruit and Green Bean Extract to increase Hb levels in pregnant women with anemia. 
 Keywords: Dragon fruit, Green Beans, Increased Hb levels, pregnancy

Pemberian Sari Kacang Hijau Untuk Meningkatkan Kadar Hemoglobin Ibu Hamil Dengan Anemia Ringan

Anemia is a condition of reduced red blood cells or commonly referred to as erythrocytes in blood circulation or hemoglobin so that they are unable to fulfill their function as oxygen carriers to all tissues (Astuti & Ertiana, 2018). Anemia can indirectly cause complications such as abortion (1.6%), premature labor (10%), infection (5.5%), causing his disorder (4%), the first stage lasts a long time (1.8%), the second stage lasts a long time (4.9%), postpartum hemorrhage (30.1%), low birth weight (62%), (Kemenkes RI, 2017). In addition to standard care, there is also care that can be given as an additional effort to increase hemoglobin levels in pregnant women, namely by consuming Ambon banana, spinach juice, honey, chicken liver, guava juice and green bean juice. According to the results of research by Dewi, et al (2017), consuming mung bean juice 2x/250ml for 14 days is very effective in increasing hemoglobin levels in pregnant women with anemia. Case Presentation: Mrs. E, 23 years old, G1P0A0Ah0, 35 weeks and 3 days of gestation, came to the health center wanting to check her condition with the main complaint recently feeling dizzy, weak and easily tired when doing daily activities. On general examination, it was found that BP: 100/60 mmHg, N: 82 x/minute, S: 36.5, R: 22 x/minute. Special examination found that the eyes and conjunctiva were slightly pale, the rest were within normal limits. In the supporting examination, the result of Hb was less than the normal limit of 10.2g/dl. Conclusion: After midwifery care was performed by providing complementary care of green bean extract 2x/250ml for 7 days, Mrs E's Hb increased from 10.2g/dl to 11.1 g/dl, eyes and conjunctiva were no longer pale.

Maillard reaction precursors and arabica coffee (Coffea arabica L.) beverage quality

The greatest diversity of aromas of coffee is formed during roasting in complex reactions such as Maillard. In order to find chemical indices of quality for the coffees, related to the Maillard reaction, the content of soluble proteins and reducing sugars (precursors of reaction) of the extracts of green beans and the respective roasted beans of 50 different coffees were evaluated. The results were related to the quality scores. For sugars, the roasted bean increase content in all samples, consequence of the breakdown of sucrose and other polysaccharides generating reducing sugars. To protein, roasted beans of coffee with quality beverage, there were a decrease in soluble proteins when comparing green beans, but in coffee of down quality, there were increase of protein in beverage. Our findings showed that the soluble protein content of the roasted beans and protein degradation during roasting significantly correlated with the quality scores of the coffees.

THE EFFECT OF GREEN BEAN JUICE AND PUDDING RED GUAVA ON THE ENHANCEMENT OF HEMOGLOBIN LEVELS IN ADOLESCENT GIRLS

Adolescent girls are at risk of developing anemia because they have menstruation every month. Prevention of anemia by consuming foods that are high in iron and foods that help the absorption process can increase the hemoglobin levels of adolescent girls. This purpose of this study was to determine the effect of consuming green bean juice and red guava pudding on the increase in hemoglobin levels of adolescent girls. This type of research used in this study was an experiment with a pre-test and post-test design. The sample in this study was 21 people with random sampling technique which was divided into three groups. The intervention was carried out for 7 days. Data analysis used paired sample t-test. The average increase after the intervention of the combination of red guava pudding and green beans was 3.6 g/dl with p<0.05 . The average increase after the intervention of the green bean extract treatment group was 2.2 g/dl with p< 0.05. While the of jelly plain group (control) only increased by 0.02 g /dl with p > 0,05 . In conclusion, this study showed that there was an effect of giving a combination of red guava pudding and green beans to increase hemoglobin levels. It was known that giving green bean juice to increase hemoglobin levels. However, there was no effect of giving agar (Control group) in increasing hemoglobin levels

Green/Roasted Coffee and Silverskin Extracts Inhibit Sugar Absorption by Human Intestinal Epithelial (Caco-2) Cells by Decreasing GLUT2 Gene Expression.

Moderate coffee ingestion has been associated with a decrease in type 2 diabetes risk, mainly due to its richness in chlorogenic acids (CGA). To explore this, extracts of green beans, roasted beans, and silverskin were prepared by aqueous ultrasound-assisted extraction and characterized by a reversed-phase high-performance liquid chromatography-photodiode array detector (RP-HPLC-DAD). The effects on the uptake of glucose and fructose by human intestinal epithelial (Caco-2) cells and the influence on the expression of sugar transporter genes (by RT-qPCR) were investigated and compared. The uptake of 3H-deoxy-D-glucose and 14C-fructose by Caco-2 cells was significantly reduced by all the extracts, with green coffee (which also contained higher amounts of CGA) achieving the highest efficiency. Although silverskin presented the lowest amounts of CGA and caffeine, it promoted an inhibitory effect similar to the effects of green/roasted beans. In the case of glucose uptake, the effect was even higher than for roasted coffee. This activity is explained by the ability of the extracts to markedly decrease GLUT2, but not GLUT5 gene expression. In addition, a decrease in SGLT1 gene expression was also found for all extracts, although not at a statistically significant rate for silverskin. This study also revealed a synergistic inhibitory effect of caffeine and 5-CQA on the uptake of sugars. Thus, silverskin appears as an interesting alternative to coffee, since the valorization of this by-product also contributes to the sustainability of the coffee chain.

Chlorogenic Acid Fractionation in Robusta Green Bean Extract as a Combination Agent of Dental Pulp Stem Cells in Periodontal Tissue Engineering

Background: Robusta coffee beans contain very high chlorogenic acid. In the last decade, chlorogenic acid was developed as an adjunct in stem cells to enhance the anti-inflammatory and antioxidant properties of stem cells when used as a therapeutic agent. Chlorogenic acid can increase proliferation and migration and inhibit the production of pro-inflammatory cytokines in stem cells thereby increasing the ability of stem cells to regenerate tissue. Purpose: To analyze the levels of chlorogenic acid in robusta coffee bean extract which can be used as a combination agent for Dental Pulp Stem Cells (DPSC) in periodontal tissue engineering therapy. Materials and Methods: Robusta coffee bean extract was obtained from the Coffee and Cocoa Research Center, Jember Regency which was processed and processed using the fractionation method. This study used DPSC with the extraction of premolar teeth of orthodontic patients. The toxicity test was performed on the coffee extract 0.0625%; 0.125%; 0.25%; 0.5% to determine the biocompatible concentration of DPSC. Results: The highest fractionation and measurement of chlorogenic acid content obtained was 30.49%. Robusta coffee bean extract with a chlorogenic acid content of 30.49% at concentrations of 0.125% and 0.0625% did not cause toxicity to DPSC. Conclusion: Chlorogenic acid in robusta coffee bean extract concentrations of 0.125% and 0.0625% are relatively biocompatible as a combination agent for DPSC in periodontal tissue engineering therapy.

The Differences of Giving Green Nuts and Vegetables to Hemoglobin (Hb) Levels in Trimester Iii Pregnant Women: A Study from Two Group Posttest Design Approach

Background: One of the complications during pregnancy is pregnant women with iron deficiency anemia. Anemia in pregnancy can be overcome by consuming foods that contain iron such as spinach and nuts. One type of legume that contains high iron is green beans (vigna radiata). Based on the survey results at the Baitussalam Community Health Center, it was shown that the number of pregnant women who had anemia was 217 people (58.96%). Pregnant women who experience anemia say that they don't consume foods that contain iron so that there is no increase in Hb levels during pregnancy.Objective: To find out the difference in giving green bean and spinach juice to hemoglobin (Hb) levels in third trimester pregnant women in the working area of the Baitussalam Community Health Center, Aceh Besar district.Materials and Methods: This type of research is experimental design with a two group posttest design approach. This research was conducted on March 17 - April 5 2020. The population and sample in this study were all pregnant women with anemia, amounting to 217 people, while the sample taken in this study amounted to 30 people consisting of two groups. The research instrument used a questionnaire and observation sheet. The data processing was carried out using statistical test paired sample t test.Results and Discussion: The results of statistical analysis showed that there were differences in the pretest and posttest in group A which were given mung bean juice to third trimester pregnant women with a p-value of 0.000. Then there were differences in the pretest and posttest in group B who were given spinach to pregnant women in the third trimester with the statistical result of p value 0.000.Conclusion: Based on the results of this study, it can be concluded that there were differences in group A who were given green bean juice and group B who were given spinach to pregnant women. trimester III. Group A which was given green bean juice had more of an increase in Hb levels compared to group B who was given spinach. Therefore, pregnant women are expected to consume green bean extract and spinach to increase Hb levels so that anemia does not occur during pregnancy.

PENGARUH PEMBERIAN SARI KACANG HIJAU (VIGNA RADIATE) TERHADAP PENINGKATAN PRODUKSI ASI PADA IBU NIFAS

Abstrack Exclusive breastfeeding is breastfeeding only for infants aged 0-6 months without providing additional food or other liquids such as formula milk, oranges, honey and tea water. In addition, additional food is also not recommended either in solid or liquid form. This study was to determine the effect of giving green bean juice (Vigna Radiate) to the increase in breast milk production in postpartum mothers at the Yeni Clinic, Tinggi Raja District, Asahan Regency in 2022. This type of research is quantitative with the design used in this study is a quasi-experimental design (Quasy Experiment) using pre and post test One Group design. Analysis of the data used is the Paired T-test. What was carried out on 10 postpartum mothers who experienced problems in exclusive breastfeeding at the Yeni Clinic, which was held in June 2022. It can be seen that those who have a score of breast milk production before being given Green Bean Extract who have a score of less than 9 people (90%), a sufficient score of 1 person (10%), and breast milk production after being given Green Bean Extract which has a production score 2 people (20%), enough breast milk production scores 8 people (80%). Based on the results of the study, the p-value is 0.000 <0.005, so it can be assumed that there is an effect of giving Green Bean Juice (vigna radiation) to the increase in breast milk production in postpartum mothers at the Yeni Clinic, Tinggi Raja District, Asahan Regency in 2022.

Chemosensoric approach for microwave- or oven-roasted Coffea arabica L. (cv. Yellow Bourbon) using electronic sensors

In this study, the chemosensory components of microwave- or oven-roasted Coffea arabica L. were compared using high-performance liquid chromatography and electronic sensors. The contents of caffeine and chlorogenic acid were the highest in green bean extract and were decreased with micro- and oven-roasting time. The decreases in the content of the two components were observed more in oven roasting. In electronic tongue analysis, green bean extract had a relatively higher bitterness, sweetness, and sourness than others. After the roasting process, the bitterness decreased at 10 min, however, increased after 15 min. In addition, sourness tended to decrease over time. Microwave-roasted extracts were lower shifted than those of oven-roasted extracts during each roasting process. Accordingly, higher changes of taste patterns were represented during oven-roasting processes. In electronic nose analysis, volatile alcohols and esters were abundant in the green bean extract, and thus, these components were considered as the main volatiles in the green bean. Through the roasting process, the number of volatiles has increased. In particular, pyrazines were generated in those of roasted samples, and the amounts of furans increased. These results provide basic data regarding the chemosensory components of Yellow Bourbon and may be utilized to optimize the roasting conditions.

Consumption of Green Bean Extract as an Effort to Increase Hemoglobin Levels in Pregnant Women

Background: Maternal mortality rate (MMR) is one of the indicators used to measure maternal health status in an area. Data in Indonesia recorded that the maternal mortality rate in 2015 was 305 per 100,000 live births, but the Sustainable Development Goals (SDG's) target is 70% per 100,000 live births in 2030. Bleeding occupies the highest percentage of maternal deaths (28%). Anemia and chronic energy deficiency (KEK) in pregnant women are the main causes of bleeding. Non-pharmacological treatment to increase hemoglobin levels by consuming foods rich in iron is green beans.
 Objectives: This study describes the effect of mung bean juice on hemoglobin levels.
 Research Metodes: This study uses a systematic review method through a literature review of 15 national journals.
 Results: The results of a literature search show that the consumption of green bean juice with the combination of consumption of FE tablets affects the increase in HB levels of pregnant women. However, this consumption must also be supported by good nutritional intake to accelerate the absorption and transport of iron and it is necessary to avoid drinks that affect iron absorption such as milk and caffeine.
 Conclusion: Green bean juice is able to significantly increase the Hb level of pregnant women with the consumption of FE tablets because the content of green beans is high in vitamin C and iron. Therefore, it is important to disseminate information to the community about the benefits and how to use mung bean juice as an effort to prevent anemia in pregnant women

Utilization of Green Bean Extract for Pregnant Women with Anemia at the Limboto Health Center, Limboto District, Gorontalo Regency

Carrying out service with the theme of Utilizing Green Bean Extract for Anemia in Pregnant Women. carried out on pregnant women in the Limboto Health Center Work Area, Limboto district, Gorontalo Regency. For pregnant women, the nutritional content of green beans can be useful in preventing anemia. Iron is one of the minerals that has an important role in the formation of red blood cells. However, nuts usually contain phytic acid which binds minerals so they cannot be absorbed optimally. The process of soaking, cooking, and germination can reduce the content of this phytic acid and increase the digestible iron. The purpose of this activity in general is to improve the degree of public health through increasing knowledge of pregnant women about the use of mung bean juice for anemic pregnant women. Demonstrating the Steps of Making Green Bean Juice

Non-polar metabolites of green beans (Phaseolus vulgaris L.) potentiate the antidiabetic activity of mesenchymal stem cells in streptozotocin-induced diabetes in rats.

Green beans (Phaseolus vulgaris L.) are consumed as pods or mature seeds (common beans). The pods were extracted with 95% ethanol and processed to prepare non-polar and polar fractions. Comparing the antihyperglycemic activity of both fractions, non-polar fraction (NPF, 200mgkg-1 day-1 ) lowered blood glucose in streptozotocin diabetic rats by 65% compared to 57% for the polar fraction at the same dose. When NPF treatment was combined with injection of mesenchymal stem cells (MSC) a 4.4-fold increase in serum insulin and a 73.6% reduction in blood glucose were observed compared to untreated control. Additionally, a significant decrease in malondialdehyde (76.2%), nitric oxide (68.2%), cholesterol (76.1%), and triglycerides (69.5%) and a 1.75-fold increase in HDL concentrations were observed in the group treated with this combination compared to diabetic animals. Interestingly, NPF increased homing of MSC in pancreas potentiating their antidiabetic activity. Finally, 26 compounds were identified in NPF using LC/MS analysis and four were isolated in pure form. The isolated compounds namely calotroproceryl acetate, fridelin, calotroproceryl A, and stigmasterol showed good inhibitory activity against pancreatic lipase with IC50 at 1.93, 1.07, 1.34 and 1.44-1μg/ml, respectively. Additionally, these compounds inhibited α-amylase, albeit at higher concentration, with IC50 at 248, 212, 254, and 155μg/ml for calotroproceryl acetate, fridelin, calotroproceryl A, and stigmasterol, respectively. Our results suggest that green beans extract can potentiate effect of MSC in diabetes directly due to its own antidiabetic effect and indirectly by increasing MSC homing in pancreatic tissues. PRACTICAL APPLICATIONS: It has been suggested in this study that green beans can improve hyperglycemia, oxidative balance in diabetes, so green beans can be promoted as a healthy nutrient for diabetic patients. Green beans also can enhance homing and differentiation of mesnchymal stem cells in the pancreas for future stem cell therapy of type I diabetes.