The Role of Robusta Green Bean Extract in Decreasing Il-1β Levels on Dental Pulp Stem Cells (Dpsc) Induced by Lps Porphyromonas Gingivalis

Abstract

Background: Stem cell-based tissue engineering is a potential strategy for periodontal tissue regeneration. Besides the advantages of stem cells as periodontal tissue regenerative therapy, this therapy must also be able to prevent and control the possibility of inflammation that occurs. Robusta green beans contain anti-inflammationsubstances that may decrease excessive inflammation in stem cell therapy. Purpose: To analyze IL-1β levels after giving Robusta green bean extract to DPSC induced by LPS P. gingivalis. Materials and Methods: This study used DPSC from the extraction of premolar teeth of orthodontic patients. The viability test was performed on the LPS P. gingivalis at 0.5; 1; 5; 10µg/ml and robusta green bean extract at 0.0625%; 0.125%; 0.25%; 0.5%to determine non toxic concentration. The treatment group was divided into 4 groups, the control group, the DPSC+LPS group, the DPSC + LPS + 0.125% Robusta green bean extract group, and the DPSC+LPS+0.0625%Robusta green bean extract group.Each group was observed at 24, 48, and 72 hours. ELISA test was performed on the supernatant to measure IL-1β levels. Results: The level of IL-1β in the Robusta green bean extract group was significantly lower (p <0.05) than the group without Robusta green bean extract at all observation times. Conclusion: Robusta green bean extract concentrations of 0.125% and 0.0625% can significantly reduce IL-1β levels in DPSC induced by LPS P. gingivalis, which is the most effective at reducing IL-1β levels was 0.125%.