Extract Of Citrullus Lanatus Research Articles

Phytochemical screening and anti-inflammatory activities of different fractions from Citrullus lanatus leaves: A comprehensive study

Inflammation, a protective immune response, can contribute to maintaining homeostasis but can also cause harm when chronic or excessive. Citrullus lanatus has been suggested to possess antioxidant and anti-inflammatory activities that may aid in preventing and treating oxidative stress and inflammation. The main objective of this study was to evaluate the levels of phytochemicals, as well as the antioxidant and anti-inflammatory activities of Citrullus lanatus leaves extract. The ethyl acetate, hexane, butanol fraction and aqueous methanol extract were subjected to various standard quantitative phytochemical analysis, while the hexane fraction and aqueous methanol extract were subjected to standard in vitro anti-inflammatory assays. Spectrophotometric and gravimetric methods were used in this study. The in vitro anti-denaturation (stabilization) assay was used for detecting a wide range of antioxidant compounds. The ethyl acetate fraction had the highest flavonoid and polyphenol levels, followed by hexane, butanol, and aqueous methanol extract, respectively. Quantitative phytochemical analysis showed the levels of flavonoids, polyphenols, tannins, alkaloids and saponins present in the different fractions of C. lanatus. The human erythrocyte membrane stabilization assay showed that extracts were able to stabilize human red blood cell membrane against hypotonic-induced hemolysis in five different samples. The extract of Citrullus lanatus was found to have the highest antioxidant activity. This study has shown that the leaves of Citrullus lanatus can be used in ethnomedicine for the prevention and treatment of ailments caused by oxidative stress and inflammation-related diseases.

The Antibacterial Effectiveness of Citrullus lanatus-Mediated Stannous Nanoparticles on Streptococcus mutans.

Introduction Dental caries is a prevalent oral health issue caused by the colonization of Streptococcus mutans in the oral cavity. Citrullus lanatus, commonly known as watermelon, is rich in bioactive compounds that possess antibacterial potential. In this study, we aimed to synthesize stannous chloride (SnCl2) nanoparticles (NPs) mediated by Citrullus lanatus extract and investigate their antibacterial effectiveness against Streptococcus mutans. Materials and method Stannous nanoparticles (SnNPs) synthesized by the green method were achieved by using the watermelon extract. Dilute stannous chloride solution was obtained by adding 0.45 gof stannous (Sn) chloride (Cl) powder to 60 mL of water, which was subjected to an orbital shaker with the watermelon extract. The nanoparticles obtained were subjected to characterization using antimicrobial testing, Fourier transform infrared (FTIR) spectroscopy, energy-dispersive X-ray (EDAX) analysis, and scanning electron microscopy (SEM). Agar well diffusion method was used against specific strains of S. aureus, S. mutans, and Escherichiacoli. Results The novel nanoparticles demonstrated promising antibacterial activity against S. mutans providing 10 mm of inhibitory action. Conclusion Due to its abundance of naturally occurring bioactive chemicals and improved efficacy against S. mutans, watermelon extract can be utilized to createstannous nanoparticles as opposed to the use of toxic chemicals. They can also be employed as oral administration systems.

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Food Science & NutritionVolume 9, Issue 9 p. 5332-5332 ERRATUMOpen Access ERRATUM This article corrects the following: Stability enhancement of lycopene in Citrullus lanatus extract via nanostructured lipid carriers Jutiporn Sirikhet, Wisinee Chanmahasathien, Araya Raiwa, Kanokwan Kiattisin, Volume 9Issue 3Food Science & Nutrition pages: 1750-1760 First Published online: January 31, 2021 First published: 15 September 2021 https://doi.org/10.1002/fsn3.2555AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat In the article by Kanokwan Kiattisin et al., (2021) there is a change in the affiliation of the corresponding author. The affiliation should be read as: 2. Innovation Center for Holistic Health, Nutraceuticals, and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand REFERENCE Sirikhet, J., Chanmahasathien, W., Raiwa, A., & Kiattisin, K. (2021). Stability enhancement of lycopene in Citrullus lanatus extract via nanostructured lipid carriers. Food Sciences and Nutrition, 9, 1750– 1760. https://doi.org/10.1002/fsn3.2156Wiley Online LibraryCASPubMedWeb of Science®Google Scholar Volume9, Issue9September 2021Pages 5332-5332 ReferencesRelatedInformation

Random decision forest based sustainable green machining using Citrullus lanatus extract as bio-cutting fluid

The presently employed synthetic cutting fluids in machining applications lead to skin irritations, hazardous aerosols, ingestion etc., to the machine tool operators. Accomplishing sustainable green machining through bio-cutting fluids, is the key motivating factor behind this work, even though the phenomenon of heat generation is inevitable in the machining process. In the present investigation, Citrullus lanatus extract is chosen as a potential candidate for bio-cutting fluid, since it offers multiple benefits such as increased tool life, cleaner production atmosphere, efficacious heat dissipation, healthy operating conditions, etc. Hybrid LM0-6SiCp-4Grp composite is selected for study since; it has potential applications in marine, aerospace, defence and automotive sectors. Experimentation is carried out with Citrullus lanatus extract as bio-cutting fluid in turning of hybrid LM0-6SiCp-4Grp composite under different processing conditions. The presence of green solution based on Citrullus lanatus extract curtails the rise of temperature significantly during turning operation. Higher magnitude of temperature decrease is observed when the percentage of the concentration of Citrullus lanatus extract is increased, irrespective of depth of turning. Bio-nature and cooling qualities of this green-cutting fluid can contribute its share for green machining and sustainable turning environment. The investigations are further progressed using machine learning algorithms based on the different categories of logical regressions implemented through the Python program. Random forest regression shows better results with 99.8% prediction accuracy compared to other machine learning approaches and the decision tree is constructed through the respective regression model. Decision tree has the ability to handle process parameters systematically and predicts the implications of Citrullus lanatus extract in decreasing the temperature at machine-tool interface during machining operation. This tree structured analysis provides a better inference that delivers flexibility in turning parameters and opens up wider options for operation. Thus, decision tree approach ensures optimized usage of production resources and increases the production capacity through judicial selection of cooling approaches associated with the turning process.

Stability enhancement of lycopene in Citrullus lanatus extract via nanostructured lipid carriers.

Lycopene is one of naturally occurring carotenoids in plants including watermelon (Citrullus lanatus). Heat, light, and oxygen effect on lycopene isomerization and degradation. Nanostructured lipid carriers (NLCs) are drug delivery system which can enhance the stability of active compound. Therefore, this study aimed to develop watermelon extract loaded in NLCs for lycopene stability improvement. The NLCs were prepared using a hot homogenization technique. Cocoa butter was used as solid lipid. Grape seed oil was used as liquid lipid. Span® 80 and Plantasens® HE20 were used as an emulsifier. The selected unloaded NLCs contained solid lipid to liquid lipid at the ratio of 3:1 and 10% (w/w) of total lipid. The particle size of watermelon extract‐loaded NLCs (WH‐loaded NLCs) was 130.17 ± 0.72 nm with low PDI and high zeta potential. It also presented high entrapment efficiency. For stability study, the WH‐NLC3 could enhance stability and maintain lycopene content after stability test. It exhibited the highest values of lycopene content (83.26 ± 2.30%) when stored at 4°C. It also possessed a prolonged release pattern over 48 hr. Therefore, the NLCs could improve stability and release profile of lycopene from watermelon extract.

Biologically green synthesis of silver nanoparticles from Citrullus lanatus extract with L-cysteine addition and investigation of colorimetric sensing of nickel(II) potential

Abstract This work presents a green synthesis of silver nanoparticles (AgNPs) using Citrullus lanatus juice extract. The biologically synthesized AgNPs and functionalized AgNPs using L-cysteine (Cys-AgNPs) were characterized by the UV-Vis spectroscopy, the FT-IR spectroscopy, and the scanning electron microscopy (SEM) analysis. The surface-plasmon resonance (SPR) bands of the AgNPs and Cys-AgNPs and appears at 420 and 415 nm, respectively. The SEM image revealed that the particles had a spherical shape and size distribution of 20–40 nm. Application of Cys-AgNPs in terms of heavy metal selectivity showed that it exhibited selectivity against nickel(II) which was confirmed by the color change from yellow to orange and the appearance of a new SPR band at 455 nm. The predicted structures of AgNPs and Cys-AgNPs showed that the complexes were formed through the electrostatic interactions and the shift of the SPR band from theoretical calculations are in accordance with experiment. Accordingly, this eco-friendly approach showed the successful application of the biologically green synthesized AgNPs modified by the L-cysteine addition for colorimetric detection of nickel(II). This proposed method could be considered as an efficient probe for the colorimetric sensing of nickel(II) with simplicity, rapidity, low cost and environmental reservation.

Antimicrobial activity of ethyl acetate extract of <i>Citrullus lanatus</i> seeds

Purpose: To determine the antimicrobial activity and chemical constituents of ethyl acetate extract of Citrullus lanatus seeds.Methods: Antimicrobial activity of the ethyl acetate extract of the seeds of C. lanatus was evaluated against Staphylococus aureus ATCC 25923, Escherichia coli ATCC 25922, Bacillus subtilis NCTC8236, Pseudomonas aeruginosa ATCC 10145 and Candida albicans ATCC 24433 using standard microbiological method. Chemical constituents of the extract were determined with Gas chromatography and mass spectrometry (GC MS) systems.Results: An oily, yellowish orange coloured extract with a yield of 18.7 % was obtained. The extract inhibited the growth of all test microorganisms at minimum inhibitory concentration range of 0.313 -2.5 mg/mL and minimum bactericidal concentration of 0.313-5 mg/mL. GC MS profile of the extract showed the presence of oleic acid (31.22 %), saturated fatty acid (23.85 %) and gamma tocopherol (8.79 %). Other organic compounds accounted for 36.16 % of the extractConclusion: Ethyl acetate extract of the seeds of Citrullus lanatus has antimicrobial effect against some Gram positive and Gram negative bacteria as well as C. albicans. Twenty-one compounds including fatty acids, hexamethyl-2-ethylacridine, gamma tocopherol and methylphthalate have been identified in the seeds.Keywords: Citrulluslanatus seeds, ethyl acetate extract, antimicrobial activity

English

The effect of methanolic extract of Citrullus lanatus seed on lipid profile and anti-oxidants in acetaminophen-induced hepatotoxic rats was investigated in the present study. A total of twenty (20) male Wistar rats were divided into five groups (n = 4). Group I served as normal control and were not exposed to paracetamol. Group II was exposed to 750 mg/kg body weight of paracetamol( intoxicated group),. Group III was administered with 0.5 ml distilled water, 5 mg/kg b. w of silymarin and group IV and V were administered with200 mg/kg and 400 mg/kg body weight of C. lanatus methanolic seed extract respectively.. We found that C. lanatus extract is safe at a dosage of up to 5000 mg/kg body weight. The biochemical analysis of current study showed significant (p 0.05) increase in VLDL, HDL and TAG concentration compared with the rats exposed to Paracetamol and 0.5 ml of distilled water (Vehicle). Moreover the animals exposed to 750 mg/kg b.w. of paracetamol and treated with 200 and 400 mg/kg b.w. of the extract showed significant (p< 0.05) increase in the antioxidant parameters compared to the animals exposed to 750 mg/kg b.w of paracetamol and0.5 ml of distilled water. The increase in the lipid profile of the treated animals reveal the ameliorating effect of dyslipidemia as observed in paracetamol intoxicated animals. Taken together methanolic seed extract of C. lanatus can substantiate its use as therapeutic agent against lipid imbalance and free radical generation in traditional system of medicine.

Evaluation of hypoglycemic effect of ethanolic seed extracts of Citrullus lanatus

Background: Diabetes is one of the most serious, chronic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. It is becoming the third “killer” of the health of mankind after cancer and cardiovascular diseases. Diabetes is a metabolic disorder affecting about 220 million people worldwide. A number of plants have been described in Ayurveda for the management of diabetes. The present study was aimed to find out the hypoglycemic and non-toxic effect of Citrullus lanatus seed extracts. Materials and Methods: In acute toxicity study, there was no mortality observed up to the maximum dose level of 2000 mg/kg body weight of the extract after administered orally. After toxicity studies the various extracts of plant were used for hypoglycaemic activity in which the ethanolic extract showed very good reduction in blood glucose level. From that three doses were selected for oral glucose tolerance test and 400 mg/kg of ethanolic seed extract showed better glycaemic control. Hence antidiabetic studies were conducted with 400 mg/kg dose level for 30 days and the tissues antioxidant levels and histopathological studies were carried out by using standard protocols. Results: The ethanolic extract of Citrullus lanatus helps to maintain the antioxidant level in various organs and also helps to protect the organs from oxidative damage. Conclusion: From our study, the ethanolic seed extract of Citrullus lanatus controls the blood glucose level and also helps to prevent the organ from oxidative damage. Hence it can be used in the management of diabetes mellitus.

Effects of Methanolic Extract of Citrullus lanatus Seed on Experimentally Induced Prostatic Hyperplasia

Aims: To investigate the effects of methanolic extract of Citrullus lanatus seed (MECLS) on experimentally induced benign prostate hyperplasia. Study design: Animal model of experimentally induced prostatic hyperplasia.