Introduction Fetal growth and development depend on maternal nutritional status and the placenta's capability to exchange nutrients, such as vitamin D, from mother to fetus. Low vitamin D status is common among healthy pregnant women and as the maternal and infant's vitamin D status is strongly correlated, interests in long-term health consequences of low maternal vitamin D status for the offspring health have increased. Objectives To examine if exposure to extra vitamin D from food fortification during fetal life influenced the risk that female offspring developed preeclampsia (PE) in their later first pregnancies. Methods In June 1985, a Danish mandatory margarine fortification policy, adding 1.25μg vitamin D/100g margarine, was terminated. This study includes women born either from June 1983 to May 1985 to mothers exposed to extra vitamin D from fortification, or from September 1986 to August 1988 to mothers not exposed to extra vitamin D from fortification, who subsequently themselves had a pregnancy. Exposed and unexposed women were followed for pregnancy until age 27.5yrs. The study population comprised 16.679 pregnant women born to exposed mothers, where 711 developed PE and 17.597 pregnant women born to unexposed mothers where 851 developed PE. All pregnancies were from 1999 to 2016.Only the woman's first pregnancy in this period was included in the study. Information on birth outcomes and PE was retrieved from the Danish National Patient Registry, and the Medical Birth Registry. Comparisons between women exposed in fetal life to extra vitamin D and women not exposed were done by a Mann–Whitney Test or X2 test. Logistic regression was used to estimate odds ratio (OR) for development of PE among exposed and un-exposed, and stratified by smoking status.