Extra Event Research Articles

P1221 Microscopic colitis and risk of venous thromboembolism: A nationwide matched cohort study

Abstract Background Inflammatory bowel diseases, including Crohn’s disease and ulcerative colitis, have been associated with an increased risk of venous thromboembolism (VTE). However, data on VTE is lacking in large population-based cohorts of microscopic colitis (MC). We aimed to investigate the association between MC and VTE. Methods We included all Swedish adults (≥18 years) with MC without previous VTE (1990-2017, n=12,489, follow-up until 2021). MC and subtypes (collagenous colitis and lymphocytic colitis) were defined from prospectively recorded colorectal histopathology reports from all 28 pathology departments in Sweden. MC patients were matched for age, sex, calendar year and county of residence with up to five reference individuals (n=55,809) without MC or previous VTE from the general population. Sensitivity analyses included full sibling comparisons and stricter definition of VTE requiring a primary diagnosis of VTE. Incidence rates and multivariable-adjusted hazard ratios (aHRs) with 95% confidence interval (CI) were calculated using Cox proportional hazards modelling. Results Over a median of 10.0 years of follow-up, 755 (6.0%; 11.3/1000 person-years [95%CI 10.5-12.1]) VTE events occurred in individuals with MC and 2674 (4.8%; 8.6/1000 person-years [8.3-8.9]) in reference individuals. Individuals with MC had a higher overall relative risk of any VTE event compared with reference individuals (aHR 1.21 [1.11-1.32]), including higher risk of pulmonary embolism (aHR 1.23 [1.08-1.40]), deep vein thrombosis of the legs (aHR 1.16 [1.03-1.32), and other VTE events (aHR 1.31 [1.08-1.58]). Compared to full siblings, individuals with MC showed numerically, but statistically non-significant, higher relative risk of VTE (aHR 1.15 [0.98-1.35]). Restricting the outcome definition to VTE diagnoses recorded as the primary diagnosis, the estimate of any VTE event was similar for MC overall (aHR 1.26 [1.15-1.39]). In the stratified analyses of VTE there were no major differences in sex, age group, follow-up time, year of MC diagnosis, country of birth, and presence/absence of celiac disease diagnosis at the index date. Conclusion In this population-based study, individuals with MC had a 21% higher risk of VTE compared with reference individuals from the general population, equal to one extra VTE event for every 37 MC individuals followed for ten years.

Characterization of a new splicing variant of powdery mildew resistance gene Pm4 in synthetic hexaploid wheat YAV249

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive fungal disease of wheat throughout the world. Utilization of effective powdery mildew resistance genes and cultivars is considered as the most economic, efficient, and environmental-friendly method to control this disease. Synthetic hexaploid wheat (SHW), which was developed through hybridization of diploid Aegilops and tetraploid wheat, is a valuable genetic resource for resistance to powdery mildew. SHW line YAV249 showed high levels of resistance to powdery mildew at both the seedling and adult stages. Genetic analysis indicated that the resistance was controlled by a single dominant gene, temporarily designated PmYAV. Bulked segregant analysis with wheat 660K single nucleotide polymorphism (SNP) array scanning and marker analysis showed that PmYAV was located on chromosome 2AL and flanked by markers Xgdm93 and Xwgrc763, respectively, with genetic distances of 0.8 cM and 1.2 cM corresponding to a physic interval of 1.89 Mb on the Chinese Spring reference genome sequence v1.0. Sequence alignment analysis demonstrated that the sequence of PmYAV was consistent with that of Pm4a but generated an extra splicing event. When inoculated with different Bgt isolates, PmYAV showed a significantly different spectrum from Pm4a, hence it might be a new resistant resource for improvement of powdery mildew resistance. The flanked markers GDM93 and WGRC763, and the co-segregated markers BCD1231 and JS717/JS718 were confirmed to be easily performed in marker-assisted selection (MAS) of PmYAV. Using MAS strategy, PmYAV was transferred into the commercial cultivar Kenong 199 (KN199) and a wheat line YK13 was derived at generation BC3F3 from the population of YAV249/4*KN199 due to its excellent agronomic traits and resistance to powdery mildew. In conclusion, an alternative splicing variant of Pm4 was identified in this study, which informed the regulation of Pm4 gene function.

Comparative genomics reveals the molecular mechanism of salt adaptation for zoysiagrasses

BackgroundZoysiagrass (Zoysia spp.) is a warm-season turfgrass. It is widely used as turfgrasses throughout the world, offers good turf qualities, including salt tolerance, resistance to drought and heat. However, the underlying genetic mechanism of zoysiagrass responsive to salt stress remains largely unexplored.ResultsIn present study, we performed a whole-genome comparative analysis for ten plant genomes. Evolutionary analysis revealed that Chloridoideae diverged from Panicoideae approximately 33.7 million years ago (Mya), and the phylogenetic relationship among three zoysiagrasses species suggested that Zoysia matrella may represent an interspecific hybrid between Zoysia japonica and Zoysia pacifica. Genomic synteny indicated that Zoysia underwent a genus-specific whole-genome duplication (WGD) event approximately 20.8 Mya. The expression bais of homologous genes between the two subgenomes suggested that the B subgenome of Z. japonica contributes to salt tolerance. In additon, comparative genomic analyses revealed that the salt adaptation of Zoysia is likely attributable to the expanded cytochrome P450 and ABA biosynthetic gene families. Furthermore, we further found that many duplicated genes from the extra WGD event exhibited distinct functional divergence in response to salt stress using transcriptomic analysis, suggesting that this WGD event contributed to strong resistance to salt stress.ConclusionsHere, our results revealed that expanded cytochrome P450 and ABA biosynthetic gene families, and many of those duplicated genes from recent zoysia-specific WGD event contributed to salt adaptation of zoysiagrass, which provided insight into the genetic underpinning of salt adaptation and valuable information for further studies on salt stress-related traits in Zoysia.

Event Forecasting for Thailand’s Car Sales during the COVID-19 Pandemic

The COVID-19 pandemic that started in 2020 has affected Thailand’s automotive industry, among many others. During the several stages of the pandemic period, car sales figures fluctuate, and hence are difficult to fit and forecast. Due to the trend present in the sales data, the Holt’s forecasting method appears a reasonable choice. However, the pandemic, or in a more general term, the “event”, requires a subtle method to handle this extra event component. This research proposes a forecasting method based on Holt’s method to better suit the time-series data affected by large-scale events. In addition, when combined with seasonality adjustment, three modified Holt’s-based methods are proposed and implemented on Thailand’s monthly car sales covering the pandemic period. Different flags are carefully assigned to each of the sales data to represent different stages of the pandemic. The results show that Holt’s method with seasonality and events yields the lowest MAPE of 8.64%, followed by 9.47% of Holt’s method with events. Compared to the typical Holt’s MAPE of 16.27%, the proposed methods are proved strongly effective for time-series data containing the event component.

Chromosome-level pepino genome provides insights into genome evolution and anthocyanin biosynthesis in Solanaceae.

Pepino (Solanum muricatum, 2n = 2x = 24), a member of the Solanaceae family, is an important globally grown fruit. Herein, we report high-quality, chromosome-level pepino genomes. The 91.67% genome sequence is anchored to 12 chromosomes, with a total length of 1.20 Gb and scaffold N50 of 87.03 Mb. More than half the genome comprises repetitive sequences. In addition to the shared ancient whole-genome triplication (WGT) event in eudicots, an additional new WGT event was present in the pepino. Our findings suggest that pepinos experienced chromosome rearrangements, fusions, and gene loss after a WGT event. The large number of gene removals indicated the instability of Solanaceae genomes, providing opportunities for species divergence and natural selection. The paucity of disease-resistance genes (NBS) in pepino and eggplant has been explained by extensive loss and limited generation of genes after WGT events in Solanaceae. The outbreak of NBS genes was not synchronized in Solanaceae species, which occurred before the Solanaceae WGT event in pepino, tomato, and tobacco, whereas it was almost synchronized with WGT events in the other four Solanaceae species. Transcriptome and comparative genomic analyses revealed several key genes involved in anthocyanin biosynthesis. Although an extra WGT event occurred in Solanaceae, CHS genes related to anthocyanin biosynthesis in grapes were still significantly expanded compared with those in Solanaceae species. Proximal and tandem duplications contributed to the expansion of CHS genes. In conclusion, the pepino genome and annotation facilitate further research into important gene functions and comparative genomic analysis in Solanaceae.

Microscopic colitis and risk of cancer - a population-based cohort study.

The association between microscopic colitis (MC) and cancer risk is unclear. Large, population-based studies are lacking. We conducted a nationwide cohort study of 11,758 patients with incident MC (diagnosed 1990-2016 in Sweden), 50,828 matched reference individuals and 11,614 siblings to MC patients. Data were obtained through Sweden´s pathology departments and from the Swedish Cancer Register. Adjusted hazard ratios (aHRs) were calculated using Cox proportional hazards models. At the end of follow up (mean: 6.7 years), 1,239 (10.5%) of MC patients had received a cancer diagnosis, compared to 4,815 (9.5%) of reference individuals (aHR 1.08 (95%CI=1.02-1.16)). The risk of cancer was highest during the first year of follow up. The absolute excess risks for cancer at 5, 10 and 20 years after MC diagnosis were +1.0% (95%CI=0.4%-1.6%), +1.5% (0.4%-2.6%) and +3.7% (-2.3-9.6%), respectively, equivalent to one extra cancer event in every 55 individuals with MC followed for ten years.MC was associated with an increased risk of lymphoma (aHR 1.43, 1.06-1.92) and lung cancer (aHR 1.32, 1.04-1.68) but with decreased risks of colorectal (aHR 0.52, 0.40-0.66) and gastrointestinal cancers (aHR 0.72, 0.60-0.85). We found no association with breast or bladder cancer. Using siblings as reference group to minimize the impact of shared genetic and early environmental factors, patients with MC were still at an increased risk of cancer (HR=1.20; 95%CI=1.06-1.36). This nationwide cohort study demonstrated an 8% increased risk of cancer in MC patients. The risk was highest during the first year of follow up.

Maternal Inheritance of U's Triangle and Evolutionary Process of Brassica Mitochondrial Genomes.

The sequences and genomic structures of plant mitochondrial (mt) genomes provide unique material for phylogenetic studies. The nature of uniparental inheritance renders an advantage when utilizing mt genomes for determining the parental sources of hybridized taxa. In this study, a concatenated matrix of mt genes was used to infer the phylogenetic relationships of six cultivated Brassica taxa and explore the maternal origins of three allotetraploids. The well-resolved sister relationships between two pairs of diploid and allotetraploid taxa suggest that Brassica carinata (car) possessed a maternal origin from Brassica nigra, while Brassica juncea (jun) was maternally derived from Brassica rapa (cam). Another allotetraploid taxon, Brassica napus (cv. Wester) may have been maternally derived from the common ancestor of B. rapa and Brassica oleracea (ole), and/or have undergone (an) extra hybridization event(s) along its evolutionary history. The characteristics of Brassica mt genomic structures also supported the phylogenetic results. Sinapis arvensis was nested inside the Brassica species, sister to the B. nigra–B. carinata lineage, and possessed an mt genome structure that mostly resembled B. nigra. Collectively, the evidence supported a systematic revision that placed S. arvensis within Brassica. Finally, ancestral mt genomes at each evolutionary node of Brassica were reconstructed, and the detailed and dynamic evolution of Brassica mt genomes was successfully reproduced. The mt genome of B. nigra structurally resembled that of the Brassica ancestor the most, with only one reversion of a block, and the Brassica oleracea underwent the most drastic changes. These findings suggested that repeat-mediated recombinations were largely responsible for the observed structural variations in the evolutionary history of Brassica mt genomes.

Bayesian Interpretation of the EXCEL Trial and Other Randomized Clinical Trials of Left Main Coronary Artery Revascularization

Patients with left main coronary artery disease have improved outcomes with coronary revascularization compared with medical therapy, but the choice of optimal revascularization technique is unresolved. To use bayesian methods to analyze the risk differences for patients with left main coronary artery disease randomized to treatment with coronary artery bypass surgery (CABG) compared with those randomized to percutaneous coronary intervention (PCI) in recent randomized clinical trials (RCTs). A systematic review using the PubMed database with the query string "(left main disease) and (PCI or CABG) and (5-year follow-up) and (clinical trial)" identified all RCTs from January 1996 to January 2020 comparing CABG to PCI for treatment of patients with left main coronary artery disease and with 5-year follow-up data. With the use of bayesian methods, the largest and most publicized RCT (EXCEL; Evaluation of XIENCE Versus Coronary Artery Bypass Surgery for Effectiveness of Left Main Revascularization; 2019) was reanalyzed (1) as an isolated entity using noninformative priors and (2) in the context of previous knowledge using informative priors derived from similar trials. Published aggregate data were used with assignments from each trial following the original intention-to-treat principle. Combining EXCEL data with varying levels of prior information using Bayes theorem provided final (posterior) probability distributions for primary and secondary outcomes. A composite end point of death, nonfatal myocardial infarction, and stroke was the primary EXCEL outcome and was accordingly the primary outcome for this reanalysis. Secondary analyses were performed for isolated all-cause mortality and for the composite outcome along with repeated revascularization procedures. When EXCEL data were analyzed using the originally stated noninferiority design, the 5-year primary outcome difference reported (2.8%; 95% CI, -0.9% to 6.5%) exceeded the predefined 4.2% noninferiority margin; thus, the null hypothesis of PCI inferiority could not be rejected. By contrast, the present bayesian analysis of the EXCEL primary outcome estimated 95% probability that the 5-year primary outcome difference was increased with PCI compared with CABG and 87% probability that this difference was greater than 1 extra event per 100 patients treated. Bayesian analyses also suggested 99% probability that EXCEL total mortality was increased with PCI and 94% probability that this absolute difference exceeded 1 extra deaths per 100 treated. A systematic review identified 3 other RCTs that studied the same question. The incorporation of this prior knowledge reduced the estimated probability of any excess mortality with PCI to 85%. For the secondary composite end point, which also included repeated revascularizations, there were estimated probabilities of 98% for at least 4 extra events and of 90% for at least 5 extra events per 100 patients treated with PCI. Bayesian analysis assisted in RCT data interpretation and specifically suggested, whether based on EXCEL results alone or on the totality of available evidence, that PCI was associated with inferior long-term results for all events, including mortality, compared with CABG for patients with left main coronary artery disease.

A Fault-Tolerant Control Strategy for T-Type Three-Level Rectifier With Neutral Point Voltage Balance and Loss Reduction

This article presents a fault-tolerant control strategy when an open-circuit fault (OCF) occurs in T-type three-level rectifiers, in which the neutral-point (NP) voltage balance and loss reduction are considered simultaneously. When an OCF occurs, one fundamental period is classified into two time intervals: the normal time interval (NTI), and abnormal time interval (ATI). In the NTI, a time-offset is utilized to achieve the NP voltage balance and loss reduction within a switching period. Aside from this, the extra switching event between the switching periods is also reduced by employing an appropriate carrier. In the ATI, the proposed method is explained by dividing fault into two conditions: the faulty condition of half-bridge switches, and NP switches. When OCF occurs at HB switches, a restriction of the time-offset is introduced during the ATI. While, if an OCF occurs at NP switches, the two-level modulation strategy is applied in the faulty phase during the ATI. Besides, the principle of carrier selection of the faulty phase is changed correspondingly to avoid the extra switching event. The performance of the fault-tolerant control with respect to the NP voltage balance and loss reduction is illustrated and verified by the simulation and experimental results.

The sensitivity of dendrogeomorphic approaches to assessing landslide movements

An understanding of past landslide behaviour is crucial for the estimation of future landslide activity. In this respect, methods of absolute dating are frequently used for landslide history assessment. Dendrogeomorphic (tree ring-based) approaches are standard methods for the spatio-temporal reconstruction of landslide activity with annual to even sub-annual resolution and timespans of several centuries (depending on the tree age). Nevertheless, the verification of the effectiveness, sensitivity and precision of these dendrogeomorphic approaches has not been addressed in more detail despite their wide spectrum of limitations. This study makes an assessment of tree ring-based chronologies of landslides based on a comparison with the independent data of past landslide activity from instrumental monitoring. The chronology of past landslide events was created from 528 tree ring series from 132 individuals of Norway spruce (Picea abies (L.) Karst.) growing on a monitored landslide in the Outer Western Carpathians (the Ondřejník Mt.) and was expressed as the yearly variation in the standard event-response index. The landslide was monitored at two sites via technical levelling (since 2007) and an automated wire extensometer (since 2014). The obtained results show a high sensitivity of reaction wood formation to movements on the order of several mm (e.g., the abrupt acceleration of movements in February 2017, as recorded by the extensometer). Moreover, a one-year delay in the growth response to movement occurring in the middle part of the growing season of the previous year was detected. This fact fully supports the introduction of the dendrological year to dendrogeomorphic research. The partial lack of correspondence between tree ring records and data from more distant monitoring sites suggests the need for separate dendrogeomorphic analyses of the individual homogenous parts of landslide areas. Finally, the use of four instead of two increment cores per tree did not provide any extra event years but increased the values of the event-response index of the dated events.

Cost-effectiveness of the non-laboratory based Framingham algorithm in primary prevention of cardiovascular disease: A simulated analysis of a cohort of African American adults

The non-lab Framingham algorithm, which substitute body mass index for lipids in the laboratory based (lab-based) Framingham algorithm, has been validated among African Americans (AAs). However, its cost-effectiveness and economic tradeoffs have not been evaluated. This study examines the incremental cost-effectiveness ratio (ICER) of two cardiovascular disease (CVD) prevention programs guided by the non-lab versus lab-based Framingham algorithm. We simulated the World Health Organization CVD prevention guidelines on a cohort of 2690 AA participants in the Atherosclerosis Risk in Communities (ARIC) cohort. Costs were estimated using Medicare fee schedules (diagnostic tests, drugs & visits), Bureau of Labor Statistics (RN wages), and estimates for managing incident CVD events. Outcomes were assumed to be true positive cases detected at a data driven treatment threshold. Both algorithms had the best balance of sensitivity/specificity at the moderate risk threshold (>10% risk). Over 12years, 82% and 77% of 401 incident CVD events were accurately predicted via the non-lab and lab-based Framingham algorithms, respectively. There were 20 fewer false negative cases in the non-lab approach translating into over $900,000 in savings over 12years. The ICER was −$57,153 for every extra CVD event prevented when using the non-lab algorithm. The approach guided by the non-lab Framingham strategy dominated the lab-based approach with respect to both costs and predictive ability. Consequently, the non-lab Framingham algorithm could potentially provide a highly effective screening tool at lower cost to address the high burden of CVD especially among AA and in resource-constrained settings where lab tests are unavailable.

A Control of Collision and Deadlock Avoidance for Automated Guided Vehicles with a Fault-Tolerance Capability

Based on a novel discrete-event zone-control model, in our previous papers [ 1 , 2 ], we presented a time-efficient traffic control for automated guided vehicle (AGV) systems to exclude inter-vehicle collisions and system deadlocks, together with a case study on container terminals. The traffic control allows each vehicle in an AGV system to freely choose its routes for any finite sequence of zone-to-zone transportation tasks and the routes can be constructed in an online fashion. In this paper, we extended our previous results with two practical goals: (1) to increase the utilization of the workspace area by reducing the minimally allowed area of each zone; (2) to avoid vehicle collisions and deadlocks with the occurrence of vehicle breakdowns. To achieve the first goal, we include one extra vehicle event that allows each vehicle to probe further ahead while it is moving on the guide-path. This leads to an extension of our previous discrete-event model and traffic control rules, which are presented in the first part of the paper. The second part of the paper concerns the second goal, for which an emergency traffic control scheme is designed as supplementary to the normal traffic control rules. As in our previous papers, the improved model and traffic control are applied to a simulation of quayside container transshipment at container terminals; our simulation results are compared with those from two interesting works in the literature.

CHARACTERIZATION OF KEPLER-91B AND THE INVESTIGATION OF A POTENTIAL TROJAN COMPANION USING EXONEST

Presented here is an independent re-analysis of the Kepler light curve of Kepler-91 (KIC 8219268). Using the EXONEST software package, which provides both Bayesian parameter estimation and Bayesian model testing, we were able to re-confirm the planetary nature of Kepler-91b. In addition to the primary and secondary eclipses of Kepler-91b, a third dimming event appears to occur approximately $60^o$ away (in phase) from the secondary eclipse, leading to the hypothesis that a Trojan planet may be located at the L4 or L5 Lagrange points. Here, we present a comprehensive investigation of four possibilities to explain the observed dimming event using all available photometric data from the Kepler Space Telescope, recently obtained radial velocity measurements, and N-body simulations. We find that the photometric model describing Kepler-91b and a Trojan planet is highly favored over the model involving Kepler-91b alone. However, it predicts an unphysically high temperature for the Trojan companion, leading to the conclusion that the extra dimming event is likely a false-postive.

Advances in Down syndrome related myeloid proliferation

Children with Down syndrome (DS) often present with transient myeloproliferative disorder (TMD) and have an increased incidence of acute megakaryocytic leukemia (AMKL). The same type of GATA1 mutation exists in these two diseases. Appearance of trisomy 21 (T21), GATA1 mutation and extra gene event in hematologic malignancies, known as three times hits theory constitute to the pathogenesis of DS related leukemia. The new notion of silent and GATA1 gene mutation monitoring can give further risk classification and better treatment to children with DS, which would enhance the whole cure rate. As a multi-step model of leukemogenesis development in vivo, the process from DS, TMD to AMKL will give us greater knowledge about how the normal hematopoietic stem/ progenitor cells transformed into leukemia cells and how the interaction between specific signaling pathway and oncogenes were. This article reviews literatures on the mechanism and related process involved in Down syndrome-related myeloid proliferation (DS-MP) will offer a theoretical basis for the further study. Key words: Down syndrome; Leukemia; Myeloproliferative disorder; GATA1 transcription factor

The circadian clock of teleost fish: a comparative analysis reveals distinct fates for duplicated genes.

The circadian clock is a central oscillator that coordinates endogenous rhythms. Members of six gene families underlie the metabolic machinery of this system. Although this machinery appears to correspond to a highly conserved genetic system in metazoans, it has been recognized that vertebrates possess a more diverse gene inventory than that of non-vertebrates. This difference could have originated in the two successive rounds of whole-genome duplications that took place in the common ancestor of the group. Teleost fish underwent an extra event of whole-genome duplication, which is thought to have provided an abundance of raw genetic material for the biological innovations that facilitated the radiation of the group. In this study, we assessed the relative contributions of whole-genome duplication and small-scale gene duplication to generate the repertoire of genes associated with the circadian clock of teleost fish. To achieve this goal, we annotated genes from six gene families associated with the circadian clock in eight teleost fish species, and we reconstructed their evolutionary history by inferring phylogenetic relationships. Our comparative analysis indicated that teleost species possess a variable repertoire of genes related to the circadian clock gene families and that the actual diversity of these genes has been shaped by a variety of phenomena, such as the complete deletion of ohnologs, the differential retention of genes, and lineage-specific gene duplications. From a functional perspective, the subfunctionalization of two ohnolog genes (PER1a and PER1b) in zebrafish highlights the power of whole-genome duplications to generate biological diversity.

Microstructural optimization of LaMg 12 alloy for hydrogen storage

A Rapid Solidification technique was applied to a LaMg 12 alloy in order to achieve refinement of the grain size. Thin ribbons were produced by solidifying the melt on a spinning copper wheel in an argon atmosphere using three different rotations speeds, 3.1, 10.5 and 20.9 m/s. The ribbons were analyzed by synchrotron X-ray diffraction (SR XRD), electron probe microanalysis (EPMA), and TEM, and they were subjected to hydrogen absorption–desorption cycling and to thermal desorption spectroscopy (TDS) characterization. SR XRD and EPMA revealed formation of two phases, LaMg 12− x and Mg. From SR XRD it was found that, depending on the cooling rate, the LaMg 12− x alloy crystallized with three different structural modifications, hexagonal TbCu 7 (highest cooling rate), tetragonal ThMn 12 (medium cooling rate) and orthorhombic LaMg 11 type (lowest cooling rate). A metastable TbCu 7-type structure (sp. gr. P6/mmm; a = 5.9617(3); c = 5.2153(5) Å) was not known from the earlier performed research and is reported for the La–Mg system for the first time. From the scanning electron microscopy (SEM) studies, RS was found to cause a significant grain refinement and an amorphisation for the highest cooling rate. The particle size of the formed hydride phases varied in the range 0.2–3 μm depending on the RS synthesis route used to prepare the original alloy. Hydrogen absorption resulted in a two-step disproportionation process: LaMg 12 + H 2 → LaH 3 + Mg → LaH 3 + MgH 2. A decrease in the grain size improved the hydrogenation kinetics. Hydrogen desorption studied by TDS and in situ SR XRD showed a major peak of hydrogen evolution at ∼370 °C. For the alloys synthesized at 10.5 m/s and 20.9 m/s, it was accompanied by an extra desorption event at 415 °C. This extra peak was associated with Mg-assisted low temperature hydrogen desorption from LaH 2 proceeding below 450 °C and leading to a recombination process to form the initial intermetallic alloy LaMg 12.

Gigantic jets with negative and positive polarity streamers

The ISUAL gigantic jets (GJs) are categorized into three types from their generating sequence and spectral properties. Generating sequence of the type I GJs resembles that reported previously; after the fully developed jet (FDJ) established the discharge channel, the ISUAL photometers registered a peak that was from a return‐stroke‐like process. The associated ULF (ultra‐low‐frequency) sferics of these type I GJs indicates that they are negative cloud‐to‐ionosphere discharges (−CIs). Type II GJs begin as blue jets and then developed into GJs in ∼100 ms. Blue jets also frequently occurred at the same region before and after the type II GJs. No identifiable ULF sferics of the type II GJs were found, though an extra event that has +CI ULF signature is probably a type II GJ. The FDJ streamer brightness of the type I GJs is ∼3.4 times of that of the type II GJs. These evidences suggest that the type II GJs are composed of positive streamers. Type III GJs were preceded by lightning, and a GJ subsequently occurred near this preceding lightning. The spectral data of the type III GJs are dominated by lightning signals and the ULF data have high background noise; thus both cannot be properly analyzed. However, the average brightness of the type III GJs falls between those of the other two types of GJs. We propose that the discharge polarity of the type III GJs can be either negative or positive, depending on the type of the charge imbalance left by the trigger lightning.

NE-11 represents a new strain variant class of Potato virus Y

This report describes the characterization by whole-genome sequencing of four PVY isolates with unique combinations of molecular and symptomatic characteristics. Three of these four isolates were of type PVY(N:O) (ID-1, OR-1, PN10A), including one of "type B", which contains an extra recombination event in the 5'UTR/P1 cistron; the other (NE-11) represents a novel PVY molecular genotype, previously misclassified as a PVY(NA-NTN) isolate. The full genome sequence of this latter isolate is unique inasmuch as it is nearly identical to that of PVY(N) isolates for the first 2,000 nucleotides (nts), after which it very strongly resembles PVY(NA-NTN) isolates for the next 600 nts. For the final 7,000 nts of its genome, NE-11 shares intermediate identity with these other two previously reported classes of PVY(N) genomes, except for a portion of the capsid protein region in which it resembles neither. Recombination in each of the four isolates was verified by a suite of recombination detection programs. PN10A represents the first complete sequence of a PVY strain variant of the class reported as PVY(N)-W (or PVY(N:O)) type B. Specific PCR assays for two unique regions of NE-11 are presented that will allow the identification of this strain variant by other researchers.

HABITUATION CONTRIBUTES TO WITHIN‐SESSION CHANGES IN FREE WHEEL RUNNING

Three experiments tested the hypothesis that habituation contributes to the regulation of wheel running. Rats ran in a wheel for 30-min sessions. Experiment 1 demonstrated spontaneous recovery. Rats ran more and the within-session decreases in running were smaller after 2 days of wheel deprivation than after 1 day. Experiment 2 demonstrated dishabituation. Running rate increased immediately after the termination of a brief extra event (application of the brake or flashing of the houselight). Experiment 3 demonstrated stimulus specificity. Rats completed the second half of the session in either the same wheel as the first half, or a different wheel. Second-half running was faster in the latter case. Within-session patterns of running were well described by equations that describe data from the habituation, motivation, and operant literatures. These results suggest that habituation contributes to the regulation of running. In fact, habituation provides a better explanation for the termination of wheel running than fatigue, the variable to which this termination is usually attributed. Overall, the present findings are consistent with the proposition that habituation and sensitization contribute to the regulation of several forms of motivated behavior.

Low-Molecular-Weight and Unfractionated Heparin for Prevention of Venous Thromboembolism in Neurosurgery

Venous thromboembolism is a common, life-threatening complication in neurosurgery, but prophylaxis with anticoagulant agents has not gained wide acceptance because of concern about intracranial bleeding. We performed a meta-analysis of controlled randomized trials on the efficacy and safety of heparin in the prophylaxis of venous thromboembolism in neurosurgery. To review the clinical benefit of prophylaxis of venous thromboembolism with heparin in the controversial setting of neurosurgery. Relevant trials evaluating heparin for prophylaxis of venous thromboembolism in neurosurgery were identified by a MEDLINE search, scan of meeting abstracts, and scrutiny of the references of original articles and reviews. Four controlled randomized studies, 3 of which involved low-molecular-weight heparin, were included in the analysis, and 4 uncontrolled studies are commented on in the article. The outcome measure (observed minus expected number of events) and its variance were calculated for each single trial and then summed. Two-tailed P values and 95% confidence intervals (CIs) were calculated. Efficacy was assessed per protocol and safety by intention-to-treat analysis. The homogeneity of the studies was tested with the chi(2) statistic. The results were also expressed as number needed for 1 extra event. A total of 187 thromboembolic events were recorded in 827 patients (22.6%). Heparin prophylaxis resulted in a 45% relative risk reduction of venous thromboembolic events (odds ratio [OR], 0.48; 95% CI, 0.35-0.66; P<. 001). Nineteen major bleedings were recorded in 1022 patients. None were fatal. Heparin treatment resulted in a 71% relative risk increase of major bleeding (OR, 1.72; 95% CI, 0.69-4.27; P =.24). The number needed to treat was 7.7 for venous thromboembolism and 16 for proximal deep vein thrombosis. The number needed to harm was 102 (115 for low-molecular-weight heparin). Low-molecular-weight and unfractionated heparin have been shown to be effective for prophylaxis of venous thromboembolism in elective neurosurgery without excessive bleeding risk.