Objective To evaluate the efficacy of CO2 laser-assisted deep sclerectomy surgery for the treatment of open angle glaucoma. Methods The data of 76 eyes of 61 patients with open angle glaucoma from Jan.2016 to Mar.2017 were collected and analyzed retrospectively. All cases were randomly divided into observed group and control group. Observed group, 32 eyes of 27 cases, received CO2 laser-assisted deep sclerectomy surgery. In observed group, there were 23 eyes of 20 males and 9 eyes of 7 females. The average age was (47.22±11.15) years.The control group, 44 eyes of 34 cases, underwent traditional trabeculectomy. There were 32 eyes of 23 males and 12 eyes of 11 females, the average age was (47.60±14.51) years in control group. The efficacy between the two groups were compared. Results The follow-up period was 8-15 months, average (9.10±2.40) months. The preoperative IOP in observed group and control group was (36.78±6.36)mmHg and (37.50±5.30)mmHg (1mmHg=0.133 kPa), respectively. The difference was not significant between the two groups (P=0.609). Six months after surgery, IOP were (15.86±2.85)mmHg and (16.61±3.31)mmHg in observed group and control group, respectively. The difference was not statistically significant between the two groups (P=0.611). IOP after surgery were lower than that before surgery (P<0.05). In observed group, the functional bleb was diffused and swelling 1week after surgery. One month after surgery, the function bleb with the coexistent modality and internal filtering type accounted for 84.38%, the external filtering accounted for 12.50%. At the last follow-up, the modality of filtering bleb was converted from the external filtering to the coexisting form. The functional bleb 6 months after surgery in the observed group was smaller than that in the control group (P=0.040). The success rate was 87.96% and 79.54% in observed group and control group respectively (P=0.395). Early postoperatively, the shallow ciliary detachment occurred in 1 eye (3.13%) in observed group. Delayed formation of anterior chamber, hyphema and choroidal detachment occurred in 20 eyes (45.45%) in control group. The difference in complication was significant between the two groups (χ2 =16.600, P=0.000). Conclusion The surgery CO2 laser-assisted deep sclerectomy is an effective and safe treatment for open angle glaucoma, with fewer complications in early postoperatively, and the internal flow of aqueous humor is dominant. Key words: Glaucoma, open-angle; Sclerectomy, deep; CO2 laser-assisted